MD03G1013000.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001307
Physical Location & Seq
Forward (+)
1031299 .. 1032482
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013000.v1.1.491

Sequence Viewer

Length: 882 bp
ATGTTGAGATTGTTGACGGGGAAGCGTCTGGTTTTTGTAGGCGATTCTCTCAACAGGAACATGTGGGAGTCTTTGCTATGTGTTCTTAGAAACTCAGTGGATGATAAGAACAAAGTCTATGAAGTTTCTGGTAGGCAAGAATTTCGCACAGAGGGCTCTTACTCCTTTGTTTTCGAGGAGTACAACTGTTCCGTGGAGTTCTTTCAATCACGGTTTCTAGTTCAAGAATGGGAGATGCTGGAACCAAGTGGATCAAAGAAGGAAACGCTTCGCATTGATTTGATTGAGAGATCCTCTGACAACTACAAAAATGCAGATGTTCTCATCTTCAACACAGGTCACTGGAAGGGTTACTATCAAGAGGGTAGCCATATTTATGGTGAACTGAATGTTGACGATGCATTTCGCAAGGCTTTAACGACATGGGCTCGATGGATAGACACCAATGTAAATCCTAAGAAAACTGCTGTTTTCTTCCGGGGCTATTCACCTTCTCACTTTAGAGGCGGAGCATGGAATTCTGGAGGGCATTGCCATGGCGAAACAAAGCCAACTACCAGCTTGGAATCCACGGAATATGGAGGAGCGTATCCAACAAACATGAAAATCTTTGACTCGGTACTCAAGGGGATGAAGACGCCAGTCAACTATCTGAACATTACAACAATGACTGATTTTCGGAAGGATGCTCATCCATCGATTTACAGAAAGCCGAATTTAACTGAGGAGGAGCGAAAACCGCCTATGATCCACGATTGCAGTCACTGGTGCCTCCCTGGCGTTCCTGATACATGGAACGTGCTAATATATGCTCAACTCCTCAAACATAACCAGAAACAACATAGACAGAAGAAACAGCAAAATCAGAGGACAACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

294

Amino Acids

34.12

Weight (kDa)

8.81

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 271 1.1e-88 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 768
AciI CCGC 2 cut(s) 507, 740
AclWI GGATC 3 cut(s) 259, 285, 742
AcsI RAATTY 3 cut(s) 140, 517, 715
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 2 cut(s) 182, 621
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 206, 224, 331
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 1 cut(s) 561
AluI AGCT 1 cut(s) 561
AlwI GGATC 3 cut(s) 259, 285, 742
AlwNI CAGNNNCTG 1 cut(s) 765
ApoI RAATTY 3 cut(s) 140, 517, 715
Asp700I GAANNNNTTC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 392, 480
BanI GGYRCC 1 cut(s) 768
BanII GRGCYC 2 cut(s) 158, 430
BbsI GAAGAC 1 cut(s) 641
BccI CCATC 2 cut(s) 426, 703
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 1 cut(s) 777
BciVI GTATCC 1 cut(s) 600
BcnI CCSGG 1 cut(s) 479
BfaI CTAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 600
BglI GCCNNNNNGGC 1 cut(s) 777
Bme1390I CCNGG 2 cut(s) 479, 777
BmiI GGNNCC 2 cut(s) 243, 770
BmrFI CCNGG 2 cut(s) 479, 777
BmsI GCATC 3 cut(s) 225, 388, 676
BoxI GACNNNNGTC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 641
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 698
BsaBI GATNNNNATC 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 638
BsaJI CCNNGG 5 cut(s) 192, 478, 535, 570, 775
Bse1I ACTGG 3 cut(s) 347, 641, 770
Bse3DI GCAATG 1 cut(s) 529
Bse8I GATNNNNATC 1 cut(s) 690
BseBI CCWGG 1 cut(s) 777
BseCI ATCGAT 1 cut(s) 698
BseDI CCNNGG 5 cut(s) 192, 478, 535, 570, 775
BseGI GGATG 4 cut(s) 106, 636, 691, 691
BseJI GATNNNNATC 1 cut(s) 690
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 108, 714
BseNI ACTGG 3 cut(s) 347, 641, 770
BseRI GAGGAG 5 cut(s) 191, 597, 740, 743, 809
BshNI GGYRCC 1 cut(s) 768
BshVI ATCGAT 1 cut(s) 698
BsiSI CCGG 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 158, 430
Bsp143I GATC 3 cut(s) 251, 290, 747
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 507, 740
BspCNI CTCAG 2 cut(s) 107, 715
BspDI ATCGAT 1 cut(s) 698
BspLI GGNNCC 2 cut(s) 243, 770
BspPI GGATC 3 cut(s) 259, 285, 742
BspT107I GGYRCC 1 cut(s) 768
BsrDI GCAATG 1 cut(s) 529
BsrI ACTGG 3 cut(s) 347, 641, 770
BssECI CCNNGG 5 cut(s) 192, 478, 535, 570, 775
BssMI GATC 3 cut(s) 251, 290, 747
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 188, 213
BstACI GRCGYC 1 cut(s) 638
BstDEI CTNAG 4 cut(s) 86, 94, 456, 723
BstDSI CCRYGG 3 cut(s) 192, 535, 570
BstF5I GGATG 4 cut(s) 106, 636, 691, 691
BstKTI GATC 3 cut(s) 254, 293, 750
BstMBI GATC 3 cut(s) 251, 290, 747
BstMWI GCNNNNNNNGC 3 cut(s) 153, 739, 777
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 64
BstPAI GACNNNNGTC 1 cut(s) 641
BstSCI CCNGG 2 cut(s) 477, 775
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 290
Bsu15I ATCGAT 1 cut(s) 698
BsuI GTATCC 1 cut(s) 600
BsuTUI ATCGAT 1 cut(s) 698
BtgI CCRYGG 3 cut(s) 192, 535, 570
BtsCI GGATG 4 cut(s) 106, 636, 691, 691
BtsIMutI CAGTG 3 cut(s) 102, 340, 763
CaiI CAGNNNCTG 1 cut(s) 765
ClaI ATCGAT 1 cut(s) 698
CseI GACGC 2 cut(s) 14, 646
Csp6I GTAC 2 cut(s) 181, 620
CviAII CATG 6 cut(s) 61, 423, 513, 536, 601, 792
CviJI RGCY 8 cut(s) 156, 369, 413, 428, 483, 550, 561, 712
CviKI_1 RGCY 8 cut(s) 156, 369, 413, 428, 483, 550, 561, 712
CviQI GTAC 2 cut(s) 181, 620
DdeI CTNAG 4 cut(s) 86, 94, 456, 723
DpnI GATC 3 cut(s) 253, 292, 749
DpnII GATC 3 cut(s) 251, 290, 747
EciI GGCGGA 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 158, 430
EcoRI GAATTC 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 535
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 158, 430
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 6 cut(s) 64, 426, 516, 539, 604, 795
FatI CATG 6 cut(s) 60, 422, 512, 535, 600, 791
FokI GGATG 4 cut(s) 113, 643, 678, 698
FriOI GRGCYC 2 cut(s) 158, 430
FspBI CTAG 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 543
HapII CCGG 1 cut(s) 478
HgaI GACGC 2 cut(s) 14, 646
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 6 cut(s) 64, 426, 516, 539, 604, 795
HincII GTYRAC 3 cut(s) 15, 394, 646
HindII GTYRAC 3 cut(s) 15, 394, 646
HinfI GANTC 4 cut(s) 44, 68, 566, 614
HpaII CCGG 1 cut(s) 478
HphI GGTGA 2 cut(s) 392, 480
Hpy166II GTNNAC 4 cut(s) 15, 383, 394, 646
Hpy188I TCNGA 4 cut(s) 298, 654, 681, 867
Hpy188III TCNNGA 4 cut(s) 224, 359, 522, 785
Hpy8I GTNNAC 4 cut(s) 15, 383, 394, 646
HpyAV CCTTC 4 cut(s) 253, 340, 501, 676
HpyCH4III ACNGT 2 cut(s) 188, 213
HpyCH4IV ACGT 1 cut(s) 798
HpyCH4V TGCA 3 cut(s) 314, 401, 759
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 739, 777
HpyF3I CTNAG 4 cut(s) 86, 94, 456, 723
HpySE526I ACGT 1 cut(s) 798
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 6 cut(s) 64, 426, 516, 539, 604, 795
Kzo9I GATC 3 cut(s) 251, 290, 747
LmnI GCTCC 3 cut(s) 509, 584, 730
LweI GCATC 3 cut(s) 225, 388, 676
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 3 cut(s) 338, 350, 761
MalI GATC 3 cut(s) 253, 292, 749
MboI GATC 3 cut(s) 251, 290, 747
MboII GAAGA 4 cut(s) 319, 466, 646, 862
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 2 cut(s) 158, 430
MluCI AATT 3 cut(s) 140, 517, 715
MlyI GAGTC 2 cut(s) 77, 608
MmeI TCCRAC 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 3 cut(s) 416, 719, 880
MslI CAYNNNNRTG 2 cut(s) 375, 534
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 2 cut(s) 479, 777
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 3 cut(s) 153, 739, 777
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 1 cut(s) 535
NdeII GATC 3 cut(s) 251, 290, 747
NlaIII CATG 6 cut(s) 64, 426, 516, 539, 604, 795
NlaIV GGNNCC 2 cut(s) 243, 770
NmuCI GTSAC 2 cut(s) 338, 761
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 64
PciI ACATGT 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 2 cut(s) 44, 566
PleI GAGTC 2 cut(s) 76, 608
PpsI GAGTC 2 cut(s) 76, 608
PscI ACATGT 1 cut(s) 60
PshAI GACNNNNGTC 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 2 cut(s) 243, 770
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 2 cut(s) 182, 621
RsaNI GTAC 2 cut(s) 181, 620
RseI CAYNNNNRTG 2 cut(s) 375, 534
SaqAI TTAA 3 cut(s) 416, 719, 880
Sau3AI GATC 3 cut(s) 251, 290, 747
SchI GAGTC 2 cut(s) 77, 608
ScrFI CCNGG 2 cut(s) 479, 777
SduI GDGCHC 2 cut(s) 158, 430
SetI ASST 4 cut(s) 340, 493, 563, 801
SfaNI GCATC 3 cut(s) 225, 388, 676
SmiMI CAYNNNNRTG 2 cut(s) 375, 534
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 3 cut(s) 140, 517, 715
SsiI CCGC 2 cut(s) 507, 740
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 477, 775
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 2 cut(s) 188, 213
TaiI ACGT 1 cut(s) 801
TaqI TCGA 3 cut(s) 174, 430, 698
TasI AATT 3 cut(s) 140, 517, 715
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 2 cut(s) 44, 566
Tru1I TTAA 3 cut(s) 416, 719, 880
Tru9I TTAA 3 cut(s) 416, 719, 880
TscAI CASTG 3 cut(s) 102, 347, 770
TseFI GTSAC 2 cut(s) 338, 761
Tsp45I GTSAC 2 cut(s) 338, 761
TspDTI ATGAA 3 cut(s) 135, 617, 647
TspGWI ACGGA 2 cut(s) 181, 587
TspRI CASTG 3 cut(s) 102, 347, 770
XapI RAATTY 3 cut(s) 140, 517, 715
XceI RCATGY 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.