Rh5AG526100

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001307
Physical Location & Seq
Reverse (-)
90254689 .. 90257005
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG526100.1

Sequence Viewer

Length: 1770 bp
ATGGCGGATTGGGGACATTCCTCAACAACAGAACACCTCTTACCAACAACCCTAGGAGAGTCGAAAGCCTCTCCTAGGCCTTCAGTACTTGCACATATCACAACAAGCACAGAATATTCCTCAATGATAAGCCCACATTCAACAAGAGCCGACACCAGCAAGGCCTCAATGTTTTCAATCTTCTTCTCCAAGAGAAATTCCATGGTTTGTACTTATGCATTCACAATTGTCTTCATTACATGTACCATCCTCTTGGTTTTCAACCCTTCTCGCCCTTCGGTTCCTTTTGGCTTCAAAAATCTTCTTTATACCTCTCCTCAGACCCCAAACCCTCATTGGAAACATGATTCTTTACCACAGTTTGATGAGATTGATAGTTTGAAAGAGAATGTGGGGATTGATCAGAAGGGATTTCAGATTGGTCTTAATCAATCTTCTGGGGGAAATGGAGGAACCAAGAAAAATGTAGAGACTGAAAGGGATGATCATGTTGGTGCTCCTCAATCATCATACACTACCAAGAAAAATGTAGAGACTGAAAGGGATGATCATATTGGTGCTCCTCAACCATCAAACACTACCATGGAAAATGTAGAGATTAAAAGGGATGATCATATTGGTACTTCTCAATCATCAAACACTACCATTCTTTCCATGTTCAAAGACTCCAATAACACAGGAACCGAAGTTTCTGAGAAAGTTGAGAGTTCTGAGAGAAGAAATGAGACCAAGAATGCTGTAATCTCTCATTCTTCTTCTAATGGAGAAAATGGTTTGAAGGAGAAAGGGAGTGATAGTAAAAAGGCACCATCTAACAAGAAACAAACTGGGTTGAAGTTGAAGAATGAATGTGACCTATTTGATGGAAGGTGGGTGAGAGATAATTCTTACCCACTTTATGCTCCAGGAACTTGTCCTTATATAGATGAGCCTTTCGATTGTTTCCACAACGGTAGGCCTGATAATGGTTACGAGAAGTACAGATGGCAACCGAAACATTGCAATGTCCCGAGGTTGGATGGTAAGAAGATGTTGAGATTGTTGGCAGGAAAGCGTCTAGTTTTTGTTGGCGATTCTCTGAATAGGAATATGTGGGAGTCTTTACTTTGTGTTCTTAGAAATTCAGTGGAAGATAAGAACAAAGTTTATGAAGTCTCGGGCAGGCAAGAGTTCCGTACGGAGGACTCTTTCTCTTTCATATTCCAGGATTATAATTGTTCAGTAGAGTTCTTTCAATCACAATTTATAGTTCAAGAATGGGAGGGGAAAGAAAAAAATGGATCCAAAAAGGAAACCCTTCGCCTTGATTTAATGGAGAGTTCCGCGGATAAATACAAAGATGCAGATATTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCTAACGGGGGTGGAGCATGGAACTCTGGAGGGCAATGCCACGGCGAGACAAAGCCAACTACAGAAACTGAATACGGAGGAGAATATTATCCATCACAAGTGAAGATCTTGGACTCAGTGATGGAGAAGATGATGACTCCAATCTTCTATCTGAACATCACAGCAATGACTGATTTCCGGAAGGATGCTCATCCATCGGTTTACAGAATGCCCAACTTAACTGCAGAGGAAAGAAAAGAGACTTCAATCCAAGATTGCAGCCACTGGTGCCTACCTGGTGTGCCTGAAACATGGAACGAGCTTGTATATGCTCAACTCCTTAGACGCAACATAAAGCAACAGAAGAAGAAGCAACATCAACAGAAGCGTCAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

589

Amino Acids

66.97

Weight (kDa)

6.82

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 283 - 335 3.3e-23 PMR5 N terminal Domain
PC-Esterase PF13839 336 - 465 2e-40 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
PC-Esterase PF13839 468 - 568 4.3e-31 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1212
AccB1I GGYRCC 2 cut(s) 805, 1659
AccII CGCG 1 cut(s) 1325
AccIII TCCGGA 1 cut(s) 1569
AciI CCGC 3 cut(s) 5, 1323, 1325
AclWI GGATC 2 cut(s) 1275, 1288
AcsI RAATTY 2 cut(s) 196, 1120
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 6 cut(s) 87, 211, 244, 622, 980, 1177
AfiI CCNNNNNNNGG 4 cut(s) 75, 965, 1015, 1180
AflIII ACRYGT 1 cut(s) 239
AjnI CCWGG 3 cut(s) 904, 1203, 1666
AluBI AGCT 1 cut(s) 1693
AluI AGCT 1 cut(s) 1693
Alw21I GWGCWC 2 cut(s) 499, 562
Alw26I GTCTC 6 cut(s) 464, 527, 719, 1159, 1433, 1625
AlwI GGATC 2 cut(s) 1275, 1288
AlwNI CAGNNNCTG 2 cut(s) 1460, 1656
Ama87I CYCGRG 2 cut(s) 1009, 1156
Aor13HI TCCGGA 1 cut(s) 1569
AoxI GGCC 3 cut(s) 77, 162, 956
ApeKI GCWGC 1 cut(s) 1650
ApoI RAATTY 2 cut(s) 196, 1120
Asp700I GAANNNNTTC 1 cut(s) 1296
AspA2I CCTAGG 2 cut(s) 52, 74
AsuHPI GGTGA 1 cut(s) 886
AvaI CYCGRG 2 cut(s) 1009, 1156
AvrII CCTAGG 2 cut(s) 52, 74
BamHI GGATCC 1 cut(s) 1280
BanI GGYRCC 2 cut(s) 805, 1659
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 2 cut(s) 499, 562
BbvI GCAGC 1 cut(s) 1662
BccI CCATC 9 cut(s) 254, 577, 817, 857, 978, 1013, 1492, 1507, 1594
BceAI ACGGC 1 cut(s) 1450
BciT130I CCWGG 3 cut(s) 906, 1205, 1668
BclI TGATCA 4 cut(s) 400, 484, 547, 610
BcoDI GTCTC 6 cut(s) 464, 527, 719, 1159, 1433, 1625
BfaI CTAG 3 cut(s) 53, 75, 1058
BfmI CTRYAG 2 cut(s) 1452, 1614
BglII AGATCT 1 cut(s) 1497
BisI GCNGC 1 cut(s) 1651
BlnI CCTAGG 2 cut(s) 52, 74
BlsI GCNGC 1 cut(s) 1652
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 3 cut(s) 906, 1205, 1668
BmeT110I CYCGRG 2 cut(s) 1009, 1156
BmiI GGNNCC 6 cut(s) 282, 454, 682, 807, 1282, 1661
BmrFI CCNGG 3 cut(s) 906, 1205, 1668
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 2 cut(s) 1330, 1567
BmuI ACTGGG 1 cut(s) 837
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 2 cut(s) 888, 1440
BsaBI GATNNNNATC 1 cut(s) 1581
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 7 cut(s) 52, 74, 201, 582, 1010, 1323, 1432
BsaWI WCCGGW 1 cut(s) 1569
BsaXI ACNNNNNCTCC 2 cut(s) 781, 811
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 965, 1015, 1180
Bse1I ACTGG 3 cut(s) 832, 1376, 1661
Bse3DI GCAATG 4 cut(s) 997, 1009, 1433, 1563
Bse8I GATNNNNATC 1 cut(s) 1581
BseAI TCCGGA 1 cut(s) 1569
BseBI CCWGG 3 cut(s) 906, 1205, 1668
BseDI CCNNGG 7 cut(s) 52, 74, 201, 582, 1010, 1323, 1432
BseGI GGATG 7 cut(s) 246, 487, 550, 613, 1024, 1582, 1582
BseJI GATNNNNATC 1 cut(s) 1581
BseLI CCNNNNNNNGG 4 cut(s) 75, 965, 1015, 1180
BseMI GCAATG 4 cut(s) 997, 1009, 1433, 1563
BseMII CTCAG 4 cut(s) 332, 684, 702, 1521
BseNI ACTGG 3 cut(s) 832, 1376, 1661
BseRI GAGGAG 4 cut(s) 306, 489, 552, 1485
BseXI GCAGC 1 cut(s) 1662
Bsh1236I CGCG 1 cut(s) 1325
BshFI GGCC 3 cut(s) 79, 164, 958
BshNI GGYRCC 2 cut(s) 805, 1659
BsiHKAI GWGCWC 2 cut(s) 499, 562
BsiHKCI CYCGRG 2 cut(s) 1009, 1156
BsiSI CCGG 1 cut(s) 1570
BsiWI CGTACG 1 cut(s) 1175
BslFI GGGAC 2 cut(s) 27, 992
BslI CCNNNNNNNGG 4 cut(s) 75, 965, 1015, 1180
BsmAI GTCTC 6 cut(s) 464, 527, 719, 1159, 1433, 1625
BsmFI GGGAC 2 cut(s) 27, 992
BsmI GAATGC 3 cut(s) 218, 739, 1605
BsnI GGCC 3 cut(s) 79, 164, 958
Bso31I GGTCTC 1 cut(s) 719
BsoBI CYCGRG 2 cut(s) 1009, 1156
Bsp1286I GDGCHC 2 cut(s) 499, 562
Bsp13I TCCGGA 1 cut(s) 1569
Bsp143I GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
Bsp19I CCATGG 2 cut(s) 201, 582
BspACI CCGC 3 cut(s) 5, 1323, 1325
BspANI GGCC 3 cut(s) 79, 164, 958
BspCNI CTCAG 4 cut(s) 331, 685, 703, 1520
BspEI TCCGGA 1 cut(s) 1569
BspFNI CGCG 1 cut(s) 1325
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 6 cut(s) 282, 454, 682, 807, 1282, 1661
BspMAI CTGCAG 1 cut(s) 1618
BspPI GGATC 2 cut(s) 1275, 1288
BspT107I GGYRCC 2 cut(s) 805, 1659
BspTNI GGTCTC 1 cut(s) 719
BsrDI GCAATG 4 cut(s) 997, 1009, 1433, 1563
BsrI ACTGG 3 cut(s) 832, 1376, 1661
BssECI CCNNGG 7 cut(s) 52, 74, 201, 582, 1010, 1323, 1432
BssMI GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
BssT1I CCWWGG 4 cut(s) 52, 74, 201, 582
Bst2UI CCWGG 3 cut(s) 906, 1205, 1668
Bst4CI ACNGT 2 cut(s) 360, 953
BstC8I GCNNGC 1 cut(s) 1163
BstDEI CTNAG 6 cut(s) 318, 693, 711, 1115, 1507, 1712
BstDSI CCRYGG 4 cut(s) 201, 582, 1323, 1432
BstENI CCTNNNNNAGG 1 cut(s) 73
BstF5I GGATG 7 cut(s) 246, 487, 550, 613, 1024, 1582, 1582
BstFNI CGCG 1 cut(s) 1325
BstKTI GATC 6 cut(s) 403, 487, 550, 613, 1283, 1500
BstMAI GTCTC 6 cut(s) 464, 527, 719, 1159, 1433, 1625
BstMBI GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
BstMWI GCNNNNNNNGC 1 cut(s) 1659
BstNI CCWGG 3 cut(s) 906, 1205, 1668
BstNSI RCATGY 1 cut(s) 243
BstSCI CCNGG 3 cut(s) 904, 1203, 1666
BstSFI CTRYAG 2 cut(s) 1452, 1614
BstUI CGCG 1 cut(s) 1325
BstV1I GCAGC 1 cut(s) 1662
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 1280, 1497
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 2 cut(s) 1280, 1497
BsuRI GGCC 3 cut(s) 79, 164, 958
BtgI CCRYGG 4 cut(s) 201, 582, 1323, 1432
BtsCI GGATG 7 cut(s) 246, 487, 550, 613, 1024, 1582, 1582
BtsIMutI CAGTG 4 cut(s) 1131, 1369, 1515, 1654
Cac8I GCNNGC 1 cut(s) 1163
CaiI CAGNNNCTG 2 cut(s) 1460, 1656
CciI TCATGA 1 cut(s) 1381
Cfr42I CCGCGG 1 cut(s) 1326
CseI GACGC 3 cut(s) 1043, 1725, 1748
CsiI ACCWGGT 1 cut(s) 1666
Csp6I GTAC 6 cut(s) 86, 210, 243, 621, 979, 1176
CviAII CATG 9 cut(s) 202, 240, 344, 488, 583, 655, 1382, 1410, 1683
CviQI GTAC 6 cut(s) 86, 210, 243, 621, 979, 1176
DdeI CTNAG 6 cut(s) 318, 693, 711, 1115, 1507, 1712
DpnI GATC 6 cut(s) 402, 486, 549, 612, 1282, 1499
DpnII GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 52, 74, 201, 582
Eco147I AGGCCT 3 cut(s) 79, 164, 958
Eco31I GGTCTC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 2 cut(s) 1009, 1156
EcoNI CCTNNNNNAGG 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 904, 1203, 1666
EcoT14I CCWWGG 4 cut(s) 52, 74, 201, 582
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 4 cut(s) 52, 74, 201, 582
FaeI CATG 9 cut(s) 205, 243, 347, 491, 586, 658, 1385, 1413, 1686
FaqI GGGAC 2 cut(s) 27, 992
FatI CATG 9 cut(s) 201, 239, 343, 487, 582, 654, 1381, 1409, 1682
FbaI TGATCA 4 cut(s) 400, 484, 547, 610
Fnu4HI GCNGC 1 cut(s) 1651
FokI GGATG 7 cut(s) 233, 494, 557, 620, 1031, 1569, 1589
Fsp4HI GCNGC 1 cut(s) 1651
FspBI CTAG 3 cut(s) 53, 75, 1058
GluI GCNGC 1 cut(s) 1651
GsuI CTGGAG 2 cut(s) 888, 1440
HaeIII GGCC 3 cut(s) 79, 164, 958
HapII CCGG 1 cut(s) 1570
HgaI GACGC 3 cut(s) 1043, 1725, 1748
Hin1II CATG 9 cut(s) 205, 243, 347, 491, 586, 658, 1385, 1413, 1686
HincII GTYRAC 1 cut(s) 1763
HindII GTYRAC 1 cut(s) 1763
HinfI GANTC 9 cut(s) 59, 347, 665, 1073, 1097, 1184, 1378, 1505, 1528
HpaII CCGG 1 cut(s) 1570
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 3 cut(s) 1377, 1594, 1763
Hpy188I TCNGA 7 cut(s) 321, 405, 417, 694, 712, 1080, 1545
Hpy188III TCNNGA 5 cut(s) 1009, 1253, 1382, 1419, 1570
Hpy8I GTNNAC 3 cut(s) 1377, 1594, 1763
HpyAV CCTTC 8 cut(s) 90, 276, 285, 400, 772, 861, 1307, 1567
HpyCH4III ACNGT 2 cut(s) 360, 953
HpyCH4V TGCA 6 cut(s) 92, 218, 1002, 1343, 1616, 1650
HpyF10VI GCNNNNNNNGC 1 cut(s) 1659
HpyF3I CTNAG 6 cut(s) 318, 693, 711, 1115, 1507, 1712
Hsp92II CATG 9 cut(s) 205, 243, 347, 491, 586, 658, 1385, 1413, 1686
Kpn2I TCCGGA 1 cut(s) 1569
Ksp22I TGATCA 4 cut(s) 400, 484, 547, 610
KspI CCGCGG 1 cut(s) 1326
Kzo9I GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
LmnI GCTCC 4 cut(s) 502, 565, 907, 1406
Lsp1109I GCAGC 1 cut(s) 1662
LweI GCATC 2 cut(s) 1330, 1567
MabI ACCWGGT 1 cut(s) 1666
MaeI CTAG 3 cut(s) 53, 75, 1058
MaeIII GTNAC 3 cut(s) 851, 968, 1367
MalI GATC 6 cut(s) 402, 486, 549, 612, 1282, 1499
MboI GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 2 cut(s) 1280, 1497
MhlI GDGCHC 2 cut(s) 499, 562
MluCI AATT 6 cut(s) 196, 225, 883, 1120, 1213, 1241
MlyI GAGTC 7 cut(s) 68, 659, 1106, 1178, 1372, 1499, 1522
MmeI TCCRAC 1 cut(s) 996
Mph1103I ATGCAT 1 cut(s) 220
MroI TCCGGA 1 cut(s) 1569
MroXI GAANNNNTTC 1 cut(s) 1296
MseI TTAA 4 cut(s) 426, 600, 1310, 1610
MslI CAYNNNNRTG 5 cut(s) 492, 555, 581, 1002, 1556
MspA1I CMGCKG 1 cut(s) 1325
MspI CCGG 1 cut(s) 1570
MspR9I CCNGG 3 cut(s) 906, 1205, 1668
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 3 cut(s) 218, 739, 1605
MvaI CCWGG 3 cut(s) 906, 1205, 1668
MvnI CGCG 1 cut(s) 1325
MwoI GCNNNNNNNGC 1 cut(s) 1659
NcoI CCATGG 2 cut(s) 201, 582
NdeII GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
NlaIII CATG 9 cut(s) 205, 243, 347, 491, 586, 658, 1385, 1413, 1686
NlaIV GGNNCC 6 cut(s) 282, 454, 682, 807, 1282, 1661
NmuCI GTSAC 2 cut(s) 851, 1367
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 243
PagI TCATGA 1 cut(s) 1381
PceI AGGCCT 3 cut(s) 79, 164, 958
PciI ACATGT 1 cut(s) 239
PctI GAATGC 3 cut(s) 218, 739, 1605
PdmI GAANNNNTTC 1 cut(s) 1296
PfeI GAWTC 2 cut(s) 347, 1073
Pfl23II CGTACG 1 cut(s) 1175
PfoI TCCNGGA 2 cut(s) 904, 1203
PkrI GCNGC 1 cut(s) 1652
PleI GAGTC 7 cut(s) 67, 659, 1105, 1178, 1372, 1499, 1522
PpsI GAGTC 7 cut(s) 67, 659, 1105, 1178, 1372, 1499, 1522
PscI ACATGT 1 cut(s) 239
PsiI TTATAA 1 cut(s) 1212
Psp6I CCWGG 3 cut(s) 904, 1203, 1666
PspGI CCWGG 3 cut(s) 904, 1203, 1666
PspLI CGTACG 1 cut(s) 1175
PspN4I GGNNCC 6 cut(s) 282, 454, 682, 807, 1282, 1661
PsrI GAACNNNNNNTAC 2 cut(s) 24, 56
PstI CTGCAG 1 cut(s) 1618
PstNI CAGNNNCTG 2 cut(s) 1460, 1656
PsuI RGATCY 2 cut(s) 1280, 1497
RsaI GTAC 6 cut(s) 87, 211, 244, 622, 980, 1177
RsaNI GTAC 6 cut(s) 86, 210, 243, 621, 979, 1176
RseI CAYNNNNRTG 5 cut(s) 492, 555, 581, 1002, 1556
SacII CCGCGG 1 cut(s) 1326
SaqAI TTAA 4 cut(s) 426, 600, 1310, 1610
SatI GCNGC 1 cut(s) 1651
Sau3AI GATC 6 cut(s) 400, 484, 547, 610, 1280, 1497
ScaI AGTACT 1 cut(s) 87
SchI GAGTC 7 cut(s) 68, 659, 1106, 1178, 1372, 1499, 1522
ScrFI CCNGG 3 cut(s) 906, 1205, 1668
SduI GDGCHC 2 cut(s) 499, 562
SetI ASST 8 cut(s) 39, 314, 858, 872, 1016, 1369, 1669, 1695
SexAI ACCWGGT 1 cut(s) 1666
SfaNI GCATC 2 cut(s) 1330, 1567
SfcI CTRYAG 2 cut(s) 1452, 1614
Sfr303I CCGCGG 1 cut(s) 1326
SgrBI CCGCGG 1 cut(s) 1326
SmiMI CAYNNNNRTG 5 cut(s) 492, 555, 581, 1002, 1556
Sse9I AATT 6 cut(s) 196, 225, 883, 1120, 1213, 1241
SseBI AGGCCT 3 cut(s) 79, 164, 958
SsiI CCGC 3 cut(s) 5, 1323, 1325
SspI AATATT 2 cut(s) 116, 1478
SspMI CTAG 3 cut(s) 53, 75, 1058
StuI AGGCCT 3 cut(s) 79, 164, 958
StyD4I CCNGG 3 cut(s) 904, 1203, 1666
StyI CCWWGG 4 cut(s) 52, 74, 201, 582
TaaI ACNGT 2 cut(s) 360, 953
TaqI TCGA 2 cut(s) 62, 936
TasI AATT 6 cut(s) 196, 225, 883, 1120, 1213, 1241
TatI WGTACW 3 cut(s) 85, 209, 978
TfiI GAWTC 2 cut(s) 347, 1073
Tru1I TTAA 4 cut(s) 426, 600, 1310, 1610
Tru9I TTAA 4 cut(s) 426, 600, 1310, 1610
TscAI CASTG 4 cut(s) 1131, 1376, 1515, 1661
TseFI GTSAC 2 cut(s) 851, 1367
TseI GCWGC 1 cut(s) 1650
Tsp45I GTSAC 2 cut(s) 851, 1367
TspDTI ATGAA 4 cut(s) 223, 861, 1164, 1186
TspGWI ACGGA 3 cut(s) 1163, 1193, 1482
TspRI CASTG 4 cut(s) 1131, 1376, 1515, 1661
XagI CCTNNNNNAGG 1 cut(s) 73
XapI RAATTY 2 cut(s) 196, 1120
XceI RCATGY 1 cut(s) 243
XcmI CCANNNNNNNNNTGG 1 cut(s) 333
XmaJI CCTAGG 2 cut(s) 52, 74
XmnI GAANNNNTTC 1 cut(s) 1296
XspI CTAG 3 cut(s) 53, 75, 1058
ZrmI AGTACT 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.