Prupe.6G013300_v2.0.a1

PMR5 N terminal Domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001307
Physical Location & Seq
Forward (+)
937302 .. 940479
3178 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G013300.1

Sequence Viewer

Length: 1833 bp
ATGGTAGATTCTCCAACCCAGACAACCCCACAATCCACAAAGCACCAGCCACCCACAATCACAAAGGCAGAGCCCAAGCCCTTGCTTTCTTCATCAATCTTCTTCAACTCAAGAAGGGCCCCCGCCCTTGCTTATGGATTCTCATTTGTCTGCATTGCCTGCACTCTCTTCTTGGTTTTCAACCCATCTGGCTACTCTTTCCCTTTCGGCTTCAAGAACATCTTCCATGCCTCCTTCTTCTACTCCTCTTCTGCCCAGACCCCAAACACCCCTTTTGAGCTTTCCCGGGAAAACCCATTTTCCTGGAAAAGTGAATCTTTACCACATTTGGATGAGAGTGGAAGTTTGAAGAGTGATCGTCAGATGGGTAATGCTCAACCACCCAACCCCATTTTTTTCATGTCTCAAGACTCCAATCAGACGTCCAAGAACACTACAATCCAAGTTTCTGGGGAACTGGAGGGTTCAAATAGAACAAATGAATCTTCCTCGTCGTCGAATGAAATTGAAGCTTTCAAGGTTAATGCGACGGAAGCTAAGCCTGTGGCTGTGAGTTCTCAATCTTTTTCTAAAGATCCTAATGGAAAAGATGGTTTGGCAAAGAATGGGGTTTTGGGGCAAGCAGAAGCAGGAAGATCAAGCTATGTTGCATTGACGTCGAAAGAGAGTGATGGTGGCAATGAGGCATTGCCTGACTCTGAGAAGCAAAGCAAACAAACTGGGCCTGGTTTGGATTTTTGGACGAATGATTGTGACATATTTGATGGAAGATGGGTGAGAGATGATTTCTACCCACTTTATGCTCCGGGGTCTTGTCCTCACATAGATGAGTCTTTCAATTGTTTTCTTAACAAGAGGCCTGATAATGGTTACGAGAAGTATAGATGGCAACCAAAACATTGCAATCTCCCAAGGTTGAATGGTAGGAACATGTTGGGATTATTGAGAGGAAAGCGTCTAGTTTTTGTAGGTGATTCTCTCAATAGGAATATGTGGGAGTCTTTAGTTTGTCTCCTCAGGAATTCAGTGGATGATAAGAGCAGAGTTGTTGAAGCCTCTGGTAGGAGCGAATTTCGTACAGAGGGCTCTCACTCTTTTATATTCAAGGATTATAATTGTTCAGTGGAGTTCTTCCGATCACCATTTCTAGTTCGAGAATGGGAGATGCCAGATGCAAATGGATCGCTAAAGGAGACACTTCGCCTTGATTTGATCGAGAGGTCCTCTGATAAGTACAAAAGTGCAGATGTTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAGAGGGAAAGGTTACTATCAAGAGGGTAGCCATATCTATGGTGAATTGAATGTTAAAGAGGCATTTCGGAAAGCTCTAATGACATGGGCAAGATGGGTTAACATCAATATAAATCTTGAGAAAACTATTGTTTTCTTCCGGGGCTATTCGCCTAATCACTTTAGAGGTGGAAGATGGAATTCTGGAGGTCAATGTCATGGTGAGACAGAGCCAACTGCAAAAGAAGCAAACAAAGGAAAATATATGTCGAAAATGAGAATCTTGGACTCGGTAATCATAGAAATGAAGATGCCAATTCTCTATCTGAACATTACAAGAATGACTGATTTTAGGAGGGATGCTCATCCATCAATTTATAGAAAGCAGAATTTCACAGAGGAGGAGAGAAAATCTCCTTTGAGATACCAGGATTGCAGTCACTGGTGCCTCCCTGGTGTTCCTGACACATGGAACGAGCTTCTGTATGCTCAGCTCATTAGACATAACCAGAAGCAACAAAAACAGCAGCATCAAGAACAGCAGCAGGGGAGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

611

Amino Acids

69.63

Weight (kDa)

8.67

Isoelectric Point (pI)

58.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1113
AatII GACGTC 2 cut(s) 425, 659
AccB1I GGYRCC 1 cut(s) 1719
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 569, 1189
AcsI RAATTY 4 cut(s) 1021, 1070, 1474, 1663
AcyI GRCGYC 2 cut(s) 422, 656
AfaI GTAC 2 cut(s) 1078, 1235
AfiI CCNNNNNNNGG 2 cut(s) 866, 1062
AflIII ACRYGT 1 cut(s) 930
AjnI CCWGG 4 cut(s) 302, 724, 1701, 1726
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AluBI AGCT 7 cut(s) 280, 512, 536, 642, 1370, 1753, 1768
AluI AGCT 7 cut(s) 280, 512, 536, 642, 1370, 1753, 1768
Alw26I GTCTC 5 cut(s) 408, 1016, 1187, 1493, 1819
AlwI GGATC 2 cut(s) 569, 1189
AlwNI CAGNNNCTG 1 cut(s) 1716
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 3 cut(s) 117, 722, 857
ApaI GGGCCC 1 cut(s) 121
ApeKI GCWGC 2 cut(s) 1801, 1816
ApoI RAATTY 4 cut(s) 1021, 1070, 1474, 1663
Asp700I GAANNNNTTC 1 cut(s) 221
AspS9I GGNCC 4 cut(s) 117, 118, 722, 1221
AsuC2I CCSGG 4 cut(s) 286, 287, 807, 1436
AsuHPI GGTGA 5 cut(s) 787, 983, 1131, 1349, 1508
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 1221
AxyI CCTNAGG 1 cut(s) 1016
BaeGI GKGCMC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 1719
BanII GRGCYC 3 cut(s) 75, 121, 1088
BbvI GCAGC 2 cut(s) 1813, 1828
BcgI CGANNNNNNTGC 4 cut(s) 639, 673, 1164, 1198
BciT130I CCWGG 4 cut(s) 304, 726, 1703, 1728
BcnI CCSGG 4 cut(s) 286, 287, 807, 1436
BcoDI GTCTC 5 cut(s) 408, 1016, 1187, 1493, 1819
BfaI CTAG 3 cut(s) 959, 1148, 1831
BisI GCNGC 2 cut(s) 1802, 1817
BlpI GCTNAGC 2 cut(s) 537, 1764
BlsI GCNGC 2 cut(s) 1803, 1818
Bme1390I CCNGG 8 cut(s) 286, 287, 304, 726, 807, 1436, 1703, 1728
Bme18I GGWCC 1 cut(s) 1221
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 117, 118, 722, 1221
BmiI GGNNCC 3 cut(s) 119, 120, 1721
BmrFI CCNGG 8 cut(s) 286, 287, 304, 726, 807, 1436, 1703, 1728
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 5 cut(s) 1155, 1162, 1575, 1624, 1813
BmuI ACTGGG 1 cut(s) 729
BplI GAGNNNNNCTC 6 cut(s) 1208, 1240, 1621, 1653, 1672, 1704
BpmI CTGGAG 2 cut(s) 479, 1500
Bpu1102I GCTNAGC 2 cut(s) 537, 1764
BpuEI CTTGAG 3 cut(s) 94, 390, 1433
BpuMI CCSGG 4 cut(s) 286, 287, 807, 1436
BsaBI GATNNNNATC 1 cut(s) 1638
BsaHI GRCGYC 2 cut(s) 422, 656
BsaI GGTCTC 1 cut(s) 1819
BsaJI CCNNGG 5 cut(s) 285, 806, 911, 1435, 1726
Bsc4I CCNNNNNNNGG 2 cut(s) 866, 1062
Bse1I ACTGG 4 cut(s) 462, 724, 1277, 1721
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 4 cut(s) 153, 685, 686, 898
Bse8I GATNNNNATC 1 cut(s) 1638
BseBI CCWGG 4 cut(s) 304, 726, 1703, 1728
BseDI CCNNGG 5 cut(s) 285, 806, 911, 1435, 1726
BseGI GGATG 4 cut(s) 337, 1036, 1639, 1639
BseJI GATNNNNATC 1 cut(s) 1638
BseLI CCNNNNNNNGG 2 cut(s) 866, 1062
BseMI GCAATG 4 cut(s) 153, 685, 686, 898
BseMII CTCAG 3 cut(s) 690, 1030, 1778
BseNI ACTGG 4 cut(s) 462, 724, 1277, 1721
BseRI GAGGAG 4 cut(s) 235, 1004, 1688, 1691
BseSI GKGCMC 1 cut(s) 121
BseXI GCAGC 2 cut(s) 1813, 1828
BsgI GTGCAG 2 cut(s) 145, 1263
BshFI GGCC 3 cut(s) 119, 724, 859
BshNI GGYRCC 1 cut(s) 1719
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 3 cut(s) 286, 806, 1435
BslI CCNNNNNNNGG 2 cut(s) 866, 1062
BsmAI GTCTC 5 cut(s) 408, 1016, 1187, 1493, 1819
BsnI GGCC 3 cut(s) 119, 724, 859
Bso31I GGTCTC 1 cut(s) 1819
BsoBI CYCGRG 1 cut(s) 285
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 3 cut(s) 75, 121, 1088
Bsp143I GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
Bsp1720I GCTNAGC 2 cut(s) 537, 1764
BspACI CCGC 1 cut(s) 123
BspANI GGCC 3 cut(s) 119, 724, 859
BspCNI CTCAG 3 cut(s) 691, 1029, 1777
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 3 cut(s) 119, 120, 1721
BspPI GGATC 2 cut(s) 569, 1189
BspT107I GGYRCC 1 cut(s) 1719
BspTNI GGTCTC 1 cut(s) 1819
BsrDI GCAATG 4 cut(s) 153, 685, 686, 898
BsrI ACTGG 4 cut(s) 462, 724, 1277, 1721
BssECI CCNNGG 5 cut(s) 285, 806, 911, 1435, 1726
BssMI GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
BssNI GRCGYC 2 cut(s) 422, 656
BssT1I CCWWGG 1 cut(s) 911
Bst2UI CCWGG 4 cut(s) 304, 726, 1703, 1728
Bst6I CTCTTC 3 cut(s) 173, 253, 344
BstACI GRCGYC 2 cut(s) 422, 656
BstAPI GCANNNNNTGC 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 160, 621
BstDEI CTNAG 4 cut(s) 537, 699, 1016, 1764
BstENI CCTNNNNNAGG 1 cut(s) 1060
BstF5I GGATG 4 cut(s) 337, 1036, 1639, 1639
BstKTI GATC 6 cut(s) 358, 577, 638, 1139, 1184, 1215
BstMAI GTCTC 5 cut(s) 408, 1016, 1187, 1493, 1819
BstMBI GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
BstMWI GCNNNNNNNGC 3 cut(s) 159, 533, 1520
BstNI CCWGG 4 cut(s) 304, 726, 1703, 1728
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 8 cut(s) 284, 285, 302, 724, 805, 1434, 1701, 1726
BstSLI GKGCMC 1 cut(s) 121
BstV1I GCAGC 2 cut(s) 1813, 1828
BstX2I RGATCY 1 cut(s) 574
BstXI CCANNNNNNTGG 3 cut(s) 303, 449, 1334
BstYI RGATCY 1 cut(s) 574
Bsu36I CCTNAGG 1 cut(s) 1016
BsuRI GGCC 3 cut(s) 119, 724, 859
BtsCI GGATG 4 cut(s) 337, 1036, 1639, 1639
BtsIMutI CAGTG 4 cut(s) 1032, 1128, 1270, 1714
Cac8I GCNNGC 2 cut(s) 160, 621
CaiI CAGNNNCTG 1 cut(s) 1716
CciI TCATGA 1 cut(s) 1282
Cfr13I GGNCC 4 cut(s) 117, 118, 722, 1221
Cfr9I CCCGGG 1 cut(s) 285
CseI GACGC 1 cut(s) 944
Csp6I GTAC 2 cut(s) 1077, 1234
CviAII CATG 7 cut(s) 227, 400, 931, 1283, 1380, 1493, 1743
CviQI GTAC 2 cut(s) 1077, 1234
DdeI CTNAG 4 cut(s) 537, 699, 1016, 1764
DpnI GATC 6 cut(s) 357, 576, 637, 1138, 1183, 1214
DpnII GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
Eam1104I CTCTTC 3 cut(s) 173, 253, 344
EarI CTCTTC 3 cut(s) 173, 253, 344
Eco130I CCWWGG 1 cut(s) 911
Eco147I AGGCCT 1 cut(s) 859
Eco24I GRGCYC 3 cut(s) 75, 121, 1088
Eco31I GGTCTC 1 cut(s) 1819
Eco47I GGWCC 1 cut(s) 1221
Eco81I CCTNAGG 1 cut(s) 1016
Eco88I CYCGRG 1 cut(s) 285
EcoNI CCTNNNNNAGG 1 cut(s) 1060
EcoO109I RGGNCCY 3 cut(s) 117, 118, 1221
EcoRI GAATTC 2 cut(s) 1021, 1474
EcoRII CCWGG 4 cut(s) 302, 724, 1701, 1726
EcoT14I CCWWGG 1 cut(s) 911
EcoT38I GRGCYC 3 cut(s) 75, 121, 1088
ErhI CCWWGG 1 cut(s) 911
FaeI CATG 7 cut(s) 230, 403, 934, 1286, 1383, 1496, 1746
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 301, 333
FatI CATG 7 cut(s) 226, 399, 930, 1282, 1379, 1492, 1742
FauI CCCGC 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 1802, 1817
FokI GGATG 4 cut(s) 344, 1043, 1626, 1646
FriOI GRGCYC 3 cut(s) 75, 121, 1088
Fsp4HI GCNGC 2 cut(s) 1802, 1817
FspBI CTAG 3 cut(s) 959, 1148, 1831
GluI GCNGC 2 cut(s) 1802, 1817
GsuI CTGGAG 2 cut(s) 479, 1500
HaeIII GGCC 3 cut(s) 119, 724, 859
HapII CCGG 3 cut(s) 286, 806, 1435
HgaI GACGC 1 cut(s) 944
Hin1I GRCGYC 2 cut(s) 422, 656
Hin1II CATG 7 cut(s) 230, 403, 934, 1286, 1383, 1496, 1746
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HindIII AAGCTT 1 cut(s) 510
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 3 cut(s) 286, 806, 1435
HphI GGTGA 5 cut(s) 787, 983, 1131, 1349, 1508
Hpy166II GTNNAC 2 cut(s) 1278, 1396
Hpy188I TCNGA 7 cut(s) 363, 420, 700, 1136, 1228, 1365, 1602
Hpy8I GTNNAC 2 cut(s) 1278, 1396
Hpy99I CGWCG 4 cut(s) 496, 499, 532, 661
HpyAV CCTTC 2 cut(s) 108, 244
HpyCH4IV ACGT 2 cut(s) 422, 656
HpyCH4V TGCA 8 cut(s) 153, 162, 650, 903, 1175, 1244, 1514, 1710
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 533, 1520
HpyF3I CTNAG 4 cut(s) 537, 699, 1016, 1764
HpySE526I ACGT 2 cut(s) 422, 656
Hsp92I GRCGYC 2 cut(s) 422, 656
Hsp92II CATG 7 cut(s) 230, 403, 934, 1286, 1383, 1496, 1746
KspAI GTTAAC 1 cut(s) 1396
Kzo9I GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
LmnI GCTCC 2 cut(s) 808, 1065
Lsp1109I GCAGC 2 cut(s) 1813, 1828
LweI GCATC 5 cut(s) 1155, 1162, 1575, 1624, 1813
MaeI CTAG 3 cut(s) 959, 1148, 1831
MaeII ACGT 2 cut(s) 422, 656
MaeIII GTNAC 5 cut(s) 752, 869, 1268, 1307, 1712
MalI GATC 6 cut(s) 357, 576, 637, 1138, 1183, 1214
MboI GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 3 cut(s) 75, 121, 1088
MlyI GAGTC 6 cut(s) 404, 689, 839, 1007, 1273, 1556
MmeI TCCRAC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 4 cut(s) 522, 849, 1350, 1395
MslI CAYNNNNRTG 4 cut(s) 330, 825, 1332, 1577
MspI CCGG 3 cut(s) 286, 806, 1435
MspR9I CCNGG 8 cut(s) 286, 287, 304, 726, 807, 1436, 1703, 1728
MunI CAATTG 1 cut(s) 838
MvaI CCWGG 4 cut(s) 304, 726, 1703, 1728
MwoI GCNNNNNNNGC 3 cut(s) 159, 533, 1520
NciI CCSGG 4 cut(s) 286, 287, 807, 1436
NdeII GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
NlaIII CATG 7 cut(s) 230, 403, 934, 1286, 1383, 1496, 1746
NlaIV GGNNCC 3 cut(s) 119, 120, 1721
NmuCI GTSAC 3 cut(s) 752, 1268, 1712
NspI RCATGY 1 cut(s) 934
PagI TCATGA 1 cut(s) 1282
PceI AGGCCT 1 cut(s) 859
PciI ACATGT 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 6 cut(s) 8, 138, 314, 482, 974, 1554
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 2 cut(s) 1803, 1818
PleI GAGTC 6 cut(s) 404, 689, 838, 1006, 1273, 1556
PpsI GAGTC 6 cut(s) 404, 689, 838, 1006, 1273, 1556
PpuMI RGGWCCY 1 cut(s) 1221
PscI ACATGT 1 cut(s) 930
PsiI TTATAA 1 cut(s) 1113
Psp5II RGGWCCY 1 cut(s) 1221
Psp6I CCWGG 4 cut(s) 302, 724, 1701, 1726
PspGI CCWGG 4 cut(s) 302, 724, 1701, 1726
PspN4I GGNNCC 3 cut(s) 119, 120, 1721
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 4 cut(s) 117, 118, 722, 1221
PspPPI RGGWCCY 1 cut(s) 1221
PstNI CAGNNNCTG 1 cut(s) 1716
PsuI RGATCY 1 cut(s) 574
RsaI GTAC 2 cut(s) 1078, 1235
RsaNI GTAC 2 cut(s) 1077, 1234
RseI CAYNNNNRTG 4 cut(s) 330, 825, 1332, 1577
SaqAI TTAA 4 cut(s) 522, 849, 1350, 1395
SatI GCNGC 2 cut(s) 1802, 1817
Sau3AI GATC 6 cut(s) 355, 574, 635, 1136, 1181, 1212
Sau96I GGNCC 4 cut(s) 117, 118, 722, 1221
SchI GAGTC 6 cut(s) 404, 689, 839, 1007, 1273, 1556
ScrFI CCNGG 8 cut(s) 286, 287, 304, 726, 807, 1436, 1703, 1728
SduI GDGCHC 3 cut(s) 75, 121, 1088
SfaNI GCATC 5 cut(s) 1155, 1162, 1575, 1624, 1813
SinI GGWCC 1 cut(s) 1221
SmaI CCCGGG 1 cut(s) 287
SmiMI CAYNNNNRTG 4 cut(s) 330, 825, 1332, 1577
SmlI CTYRAG 3 cut(s) 109, 405, 1412
SmoI CTYRAG 3 cut(s) 109, 405, 1412
SseBI AGGCCT 1 cut(s) 859
SsiI CCGC 1 cut(s) 123
SspMI CTAG 3 cut(s) 959, 1148, 1831
StuI AGGCCT 1 cut(s) 859
StyD4I CCNGG 8 cut(s) 284, 285, 302, 724, 805, 1434, 1701, 1726
StyI CCWWGG 1 cut(s) 911
TaiI ACGT 2 cut(s) 425, 659
TaqI TCGA 5 cut(s) 497, 659, 1153, 1215, 1544
TatI WGTACW 1 cut(s) 1233
TfiI GAWTC 6 cut(s) 8, 138, 314, 482, 974, 1554
Tru1I TTAA 4 cut(s) 522, 849, 1350, 1395
Tru9I TTAA 4 cut(s) 522, 849, 1350, 1395
TscAI CASTG 4 cut(s) 1032, 1128, 1277, 1721
TseFI GTSAC 3 cut(s) 752, 1268, 1712
TseI GCWGC 2 cut(s) 1801, 1816
Tsp45I GTSAC 3 cut(s) 752, 1268, 1712
TspDTI ATGAA 5 cut(s) 81, 388, 495, 516, 1595
TspGWI ACGGA 1 cut(s) 545
TspMI CCCGGG 1 cut(s) 285
TspRI CASTG 4 cut(s) 1032, 1128, 1277, 1721
VpaK11BI GGWCC 1 cut(s) 1221
XagI CCTNNNNNAGG 1 cut(s) 1060
XapI RAATTY 4 cut(s) 1021, 1070, 1474, 1663
XceI RCATGY 1 cut(s) 934
XmaI CCCGGG 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 3 cut(s) 959, 1148, 1831
ZraI GACGTC 2 cut(s) 423, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.