Rh5AG526000

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001307
Physical Location & Seq
Reverse (-)
90241914 .. 90244533
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG526000.1

Sequence Viewer

Length: 1602 bp
ATGGCAGATTCCTCACCCCAAACTACAAAGCACCACTCACCCACTAAAGCAGAGCCCAAGGCTTCTCTTTCTTCCATCTTCTTCACTTCAAGAAAAGCCTCCGCCTTTGCTTATGGAATCGCCTTTGCCCTCATTGCCTTCACTTCCTTCTGGGTTTTCAACCCATCTGGCTACTCTTTCCCTTTTAGCTTCAACACCATCTTCTACTCTTCTCCTTCCAACTCTTCTCAGGAAGTTACTAAATCCCCAAAGATTGAATCTTTACCACATTTGGGCAGCAATGGAAGTTTGAACAGTGAAATGGGTGCTTCTCAGTTTTCCGGGAAGATGGGAAATCAGAGCAGTACTCAAATGGGTACTGATCAATCTTCAAAACCCGTGATTTCTTTGCCCCAAGAATCCAATCAGACCAGAAATGAGTCTTCTTTTTCTGAGGGTTCTAGTGGGGAAAATGGTTTGCCAAACTATGTGGCATTGAAGTCCATAAGCAGTAAGGGATTGTCTGGGTTTGAGTTGATGAATGGTTGTAATTTGTTTGATGGAAGATGGGTGAGAGATGATTCATACCCACTTTATGCTGCTGGGTCTTGTCCTTACATTGATGAATCTTTCAATTGTTTTCTCAATAGTAGGCCTGATAATGGTTATGAAAAGTATAGATGGCAACCCAAAAACTGCAATCTCCCTAGGATGAATGGAAGGAACATGTTGGAATTATTGAGAGGAAAGCGTCTAGTTTTTGTTGGTGATTCTCTCAACAGGAATATGTGGGAGTCTTTGGTTTGTCTTCTTAGGAATTCAGTGGATGATAAGAGCAGAGTTTTTGAAGCTTCTGGCAGGAGCAAATTTCGTACAGACGGCGCCTACTTTTTTGTATTCAAGGATTACAATTGTTCACTGGAGTTTGTTCAATCACCATTTCTAGTTCAAGAATTAGAGGTGCTAACCACAAATGGGTCAAAGAAGGAAACACTGCGCCTGGATATGATTCAGAGATCCTTGGATAAGTACAAAAGTGCAGATGTTCTCGTCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAGAGGGCAGGGTTACTATCAAGAGGGTGACTATATCTATAGTCAATTGAATGTCAAAGAGGCGTATGGAAAAGCTATAACAACATGGGCCAGATGGCTGGATGCTAATATAAATCCTGAAAAAACTGTTAACCTCATGGATGGTTTACAACTATACAACATCTTCATGTTTGCTTTACCATTTCATAGTGGTGGAAGATGGAATACTGGAGGTCAATGTCATGGTCGGACAGAGCCAATTCAATACGAGGCATACAAAGGAAAATATCCTGCAAAGATGAGAATACTGGACTCAGTAATCAGGGAAATGAAGATTCCAATCTTTTATCTGAATGTTACAAAAATGACTGATTTTCGGAGGGATGCTCACCCATCCATTTACAGAAAGCAGAATTTGACAGAGGAGGAGAGACAGTTGTCTTTGAGATCCCAGGATTGCAGTCATTGGTGCCTCCCTGGTGTTCCTGATACATGGAACGAGCTTCTATATGCTCATCTCCTTACATATACCCAGCAGCAGAGGAGACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

533

Amino Acids

60.74

Weight (kDa)

8.82

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 175 - 227 2.1e-22 PMR5 N terminal Domain
PC-Esterase PF13839 228 - 521 6.8e-91 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 860, 1518
AciI CCGC 1 cut(s) 102
AclWI GGATC 2 cut(s) 990, 1491
AcsI RAATTY 3 cut(s) 796, 845, 1462
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 4 cut(s) 346, 358, 853, 1010
AfiI CCNNNNNNNGG 3 cut(s) 272, 641, 954
AflIII ACRYGT 1 cut(s) 705
AjnI CCWGG 3 cut(s) 978, 1500, 1525
AluBI AGCT 4 cut(s) 189, 830, 1145, 1552
AluI AGCT 4 cut(s) 189, 830, 1145, 1552
Alw26I GTCTC 2 cut(s) 1474, 1588
AlwI GGATC 2 cut(s) 990, 1491
AoxI GGCC 2 cut(s) 632, 1158
ApeKI GCWGC 3 cut(s) 276, 578, 1585
ApoI RAATTY 3 cut(s) 796, 845, 1462
AspA2I CCTAGG 1 cut(s) 686
AspLEI GCGC 2 cut(s) 863, 978
AspS9I GGNCC 1 cut(s) 1158
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 7 cut(s) 6, 30, 562, 758, 906, 1109, 1430
AvrII CCTAGG 1 cut(s) 686
BanI GGYRCC 2 cut(s) 860, 1518
BanII GRGCYC 1 cut(s) 57
BarI GAAGNNNNNNTAC 2 cut(s) 1258, 1290
BbsI GAAGAC 3 cut(s) 414, 779, 1024
BbvI GCAGC 3 cut(s) 288, 565, 1597
BceAI ACGGC 1 cut(s) 874
BciT130I CCWGG 3 cut(s) 980, 1502, 1527
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 1474, 1588
BfaI CTAG 4 cut(s) 441, 687, 734, 923
BfmI CTRYAG 1 cut(s) 1108
BfoI RGCGCY 1 cut(s) 864
BisI GCNGC 3 cut(s) 277, 579, 1586
BlnI CCTAGG 1 cut(s) 686
BlsI GCNGC 3 cut(s) 278, 580, 1587
BmcAI AGTACT 1 cut(s) 346
Bme1390I CCNGG 4 cut(s) 322, 980, 1502, 1527
BmgT120I GGNCC 1 cut(s) 1158
BmiI GGNNCC 2 cut(s) 862, 1520
BmrFI CCNGG 4 cut(s) 322, 980, 1502, 1527
BmsI GCATC 2 cut(s) 1162, 1423
BoxI GACNNNNGTC 1 cut(s) 1486
BpiI GAAGAC 3 cut(s) 414, 779, 1024
BplI GAGNNNNNCTC 4 cut(s) 331, 363, 1420, 1452
BpmI CTGGAG 2 cut(s) 920, 1299
BpuMI CCSGG 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 1388
BsaHI GRCGYC 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 1588
BsaJI CCNNGG 5 cut(s) 57, 686, 999, 1500, 1525
BsaXI ACNNNNNCTCC 2 cut(s) 1469, 1499
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 641, 954
Bse1I ACTGG 4 cut(s) 903, 1052, 1282, 1362
Bse3DI GCAATG 2 cut(s) 132, 286
Bse8I GATNNNNATC 1 cut(s) 1388
BseBI CCWGG 3 cut(s) 980, 1502, 1527
BseDI CCNNGG 5 cut(s) 57, 686, 999, 1500, 1525
BseGI GGATG 6 cut(s) 696, 811, 1177, 1216, 1438, 1442
BseJI GATNNNNATC 1 cut(s) 1388
BseLI CCNNNNNNNGG 3 cut(s) 272, 641, 954
BseMI GCAATG 2 cut(s) 132, 286
BseMII CTCAG 4 cut(s) 242, 326, 423, 1377
BseNI ACTGG 4 cut(s) 903, 1052, 1282, 1362
BseRI GAGGAG 2 cut(s) 1487, 1490
BseXI GCAGC 3 cut(s) 288, 565, 1597
BseYI CCCAGC 2 cut(s) 581, 1581
BsgI GTGCAG 1 cut(s) 1038
BshFI GGCC 2 cut(s) 634, 1160
BshNI GGYRCC 2 cut(s) 860, 1518
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 3 cut(s) 272, 641, 954
BsmAI GTCTC 2 cut(s) 1474, 1588
BsnI GGCC 2 cut(s) 634, 1160
Bso31I GGTCTC 1 cut(s) 1588
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 3 cut(s) 361, 995, 1496
BspACI CCGC 1 cut(s) 102
BspANI GGCC 2 cut(s) 634, 1160
BspCNI CTCAG 4 cut(s) 241, 325, 424, 1376
BspHI TCATGA 1 cut(s) 1057
BspLI GGNNCC 2 cut(s) 862, 1520
BspPI GGATC 2 cut(s) 990, 1491
BspT107I GGYRCC 2 cut(s) 860, 1518
BspTNI GGTCTC 1 cut(s) 1588
BsrDI GCAATG 2 cut(s) 132, 286
BsrI ACTGG 4 cut(s) 903, 1052, 1282, 1362
BssECI CCNNGG 5 cut(s) 57, 686, 999, 1500, 1525
BssMI GATC 3 cut(s) 361, 995, 1496
BssNI GRCGYC 1 cut(s) 861
BssT1I CCWWGG 3 cut(s) 57, 686, 999
Bst2UI CCWGG 3 cut(s) 980, 1502, 1527
Bst4CI ACNGT 3 cut(s) 296, 1198, 1485
Bst6I CTCTTC 2 cut(s) 214, 229
BstACI GRCGYC 1 cut(s) 861
BstDEI CTNAG 5 cut(s) 228, 312, 432, 791, 1363
BstF5I GGATG 6 cut(s) 696, 811, 1177, 1216, 1438, 1442
BstH2I RGCGCY 1 cut(s) 864
BstHHI GCGC 2 cut(s) 863, 978
BstKTI GATC 3 cut(s) 364, 998, 1499
BstMAI GTCTC 2 cut(s) 1474, 1588
BstMBI GATC 3 cut(s) 361, 995, 1496
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 3 cut(s) 980, 1502, 1527
BstNSI RCATGY 1 cut(s) 709
BstPAI GACNNNNGTC 1 cut(s) 1486
BstSCI CCNGG 4 cut(s) 320, 978, 1500, 1525
BstSFI CTRYAG 1 cut(s) 1108
BstV1I GCAGC 3 cut(s) 288, 565, 1597
BstV2I GAAGAC 3 cut(s) 414, 779, 1024
BstX2I RGATCY 2 cut(s) 995, 1496
BstXI CCANNNNNNTGG 1 cut(s) 1168
BstYI RGATCY 2 cut(s) 995, 1496
BsuRI GGCC 2 cut(s) 634, 1160
BtsCI GGATG 6 cut(s) 696, 811, 1177, 1216, 1438, 1442
BtsI GCAGTG 1 cut(s) 971
BtsIMutI CAGTG 5 cut(s) 301, 807, 896, 971, 1045
CciI TCATGA 1 cut(s) 1057
CfoI GCGC 2 cut(s) 863, 978
Cfr13I GGNCC 1 cut(s) 1158
CseI GACGC 1 cut(s) 719
Csp6I GTAC 4 cut(s) 345, 357, 852, 1009
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviAII CATG 7 cut(s) 706, 1058, 1155, 1207, 1237, 1292, 1542
CviQI GTAC 4 cut(s) 345, 357, 852, 1009
DdeI CTNAG 5 cut(s) 228, 312, 432, 791, 1363
DinI GGCGCC 1 cut(s) 862
DpnI GATC 3 cut(s) 363, 997, 1498
DpnII GATC 3 cut(s) 361, 995, 1496
Eam1104I CTCTTC 2 cut(s) 214, 229
EarI CTCTTC 2 cut(s) 214, 229
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 3 cut(s) 57, 686, 999
Eco147I AGGCCT 1 cut(s) 634
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 1588
EcoRI GAATTC 1 cut(s) 796
EcoRII CCWGG 3 cut(s) 978, 1500, 1525
EcoT14I CCWWGG 3 cut(s) 57, 686, 999
EcoT38I GRGCYC 1 cut(s) 57
EgeI GGCGCC 1 cut(s) 862
EheI GGCGCC 1 cut(s) 862
ErhI CCWWGG 3 cut(s) 57, 686, 999
FaeI CATG 7 cut(s) 709, 1061, 1158, 1210, 1240, 1295, 1545
FatI CATG 7 cut(s) 705, 1057, 1154, 1206, 1236, 1291, 1541
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 3 cut(s) 277, 579, 1586
FokI GGATG 6 cut(s) 703, 818, 1184, 1223, 1429, 1445
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 3 cut(s) 277, 579, 1586
FspBI CTAG 4 cut(s) 441, 687, 734, 923
GlaI GCGC 2 cut(s) 862, 977
GluI GCNGC 3 cut(s) 277, 579, 1586
GsaI CCCAGC 2 cut(s) 585, 1585
GsuI CTGGAG 2 cut(s) 920, 1299
HaeII RGCGCY 1 cut(s) 864
HaeIII GGCC 2 cut(s) 634, 1160
HapII CCGG 1 cut(s) 321
HgaI GACGC 1 cut(s) 719
HhaI GCGC 2 cut(s) 863, 978
Hin1I GRCGYC 1 cut(s) 861
Hin1II CATG 7 cut(s) 709, 1061, 1158, 1210, 1240, 1295, 1545
Hin6I GCGC 2 cut(s) 861, 976
HinP1I GCGC 2 cut(s) 861, 976
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 828
HpaI GTTAAC 1 cut(s) 1201
HpaII CCGG 1 cut(s) 321
HphI GGTGA 7 cut(s) 6, 30, 562, 758, 906, 1109, 1430
Hpy166II GTNNAC 4 cut(s) 896, 1053, 1201, 1217
Hpy188I TCNGA 7 cut(s) 339, 408, 433, 993, 1299, 1401, 1428
Hpy188III TCNNGA 8 cut(s) 90, 230, 929, 1058, 1071, 1091, 1187, 1535
Hpy8I GTNNAC 4 cut(s) 896, 1053, 1201, 1217
HpyAV CCTTC 5 cut(s) 148, 157, 225, 693, 958
HpyCH4III ACNGT 3 cut(s) 296, 1198, 1485
HpyCH4V TGCA 4 cut(s) 678, 1019, 1343, 1509
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 5 cut(s) 228, 312, 432, 791, 1363
Hsp92I GRCGYC 1 cut(s) 861
Hsp92II CATG 7 cut(s) 709, 1061, 1158, 1210, 1240, 1295, 1545
HspAI GCGC 2 cut(s) 861, 976
KasI GGCGCC 1 cut(s) 860
Ksp22I TGATCA 1 cut(s) 361
KspAI GTTAAC 1 cut(s) 1201
Kzo9I GATC 3 cut(s) 361, 995, 1496
LmnI GCTCC 1 cut(s) 840
Lsp1109I GCAGC 3 cut(s) 288, 565, 1597
LweI GCATC 2 cut(s) 1162, 1423
MaeI CTAG 4 cut(s) 441, 687, 734, 923
MaeIII GTNAC 5 cut(s) 235, 1043, 1082, 1097, 1405
MalI GATC 3 cut(s) 363, 997, 1498
MboI GATC 3 cut(s) 361, 995, 1496
MfeI CAATTG 3 cut(s) 613, 889, 1115
MflI RGATCY 2 cut(s) 995, 1496
MhlI GDGCHC 1 cut(s) 57
Mly113I GGCGCC 1 cut(s) 861
MlyI GAGTC 4 cut(s) 428, 782, 1048, 1355
MmeI TCCRAC 3 cut(s) 243, 690, 1277
MseI TTAA 1 cut(s) 1200
MslI CAYNNNNRTG 2 cut(s) 1235, 1260
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 4 cut(s) 322, 980, 1502, 1527
MunI CAATTG 3 cut(s) 613, 889, 1115
MvaI CCWGG 3 cut(s) 980, 1502, 1527
MwoI GCNNNNNNNGC 1 cut(s) 134
NarI GGCGCC 1 cut(s) 861
NciI CCSGG 1 cut(s) 322
NdeII GATC 3 cut(s) 361, 995, 1496
NlaIII CATG 7 cut(s) 709, 1061, 1158, 1210, 1240, 1295, 1545
NlaIV GGNNCC 2 cut(s) 862, 1520
NmuCI GTSAC 2 cut(s) 1043, 1097
NspI RCATGY 1 cut(s) 709
PagI TCATGA 1 cut(s) 1057
PceI AGGCCT 1 cut(s) 634
PciI ACATGT 1 cut(s) 705
PfeI GAWTC 9 cut(s) 8, 117, 257, 398, 560, 605, 749, 988, 1384
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 3 cut(s) 278, 580, 1587
PleI GAGTC 4 cut(s) 427, 781, 1048, 1355
PluTI GGCGCC 1 cut(s) 864
PpsI GAGTC 4 cut(s) 427, 781, 1048, 1355
PscI ACATGT 1 cut(s) 705
PshAI GACNNNNGTC 1 cut(s) 1486
Psp6I CCWGG 3 cut(s) 978, 1500, 1525
PspFI CCCAGC 2 cut(s) 581, 1581
PspGI CCWGG 3 cut(s) 978, 1500, 1525
PspN4I GGNNCC 2 cut(s) 862, 1520
PspPI GGNCC 1 cut(s) 1158
PsuI RGATCY 2 cut(s) 995, 1496
RsaI GTAC 4 cut(s) 346, 358, 853, 1010
RsaNI GTAC 4 cut(s) 345, 357, 852, 1009
RseI CAYNNNNRTG 2 cut(s) 1235, 1260
SaqAI TTAA 1 cut(s) 1200
SatI GCNGC 3 cut(s) 277, 579, 1586
Sau3AI GATC 3 cut(s) 361, 995, 1496
Sau96I GGNCC 1 cut(s) 1158
ScaI AGTACT 1 cut(s) 346
SchI GAGTC 4 cut(s) 428, 782, 1048, 1355
ScrFI CCNGG 4 cut(s) 322, 980, 1502, 1527
SduI GDGCHC 1 cut(s) 57
SetI ASST 8 cut(s) 191, 832, 942, 1045, 1147, 1206, 1285, 1554
SfaNI GCATC 2 cut(s) 1162, 1423
SfcI CTRYAG 1 cut(s) 1108
SfoI GGCGCC 1 cut(s) 862
SmiMI CAYNNNNRTG 2 cut(s) 1235, 1260
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 1 cut(s) 102
SspDI GGCGCC 1 cut(s) 860
SspMI CTAG 4 cut(s) 441, 687, 734, 923
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 4 cut(s) 320, 978, 1500, 1525
StyI CCWWGG 3 cut(s) 57, 686, 999
TaaI ACNGT 3 cut(s) 296, 1198, 1485
TatI WGTACW 2 cut(s) 344, 1008
TfiI GAWTC 9 cut(s) 8, 117, 257, 398, 560, 605, 749, 988, 1384
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 5 cut(s) 301, 807, 903, 978, 1052
TseFI GTSAC 2 cut(s) 1043, 1097
TseI GCWGC 3 cut(s) 276, 578, 1585
Tsp45I GTSAC 2 cut(s) 1043, 1097
TspDTI ATGAA 8 cut(s) 533, 552, 618, 663, 707, 1225, 1244, 1394
TspRI CASTG 5 cut(s) 301, 807, 903, 978, 1052
XapI RAATTY 3 cut(s) 796, 845, 1462
XceI RCATGY 1 cut(s) 709
XmaJI CCTAGG 1 cut(s) 686
XspI CTAG 4 cut(s) 441, 687, 734, 923
ZrmI AGTACT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.