MD03G1013500.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001307
Physical Location & Seq
Forward (+)
1066988 .. 1069795
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013500.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGCGGATTACTCAACCCAGACAACCCCAAAACCCACAAAGCACCACCACTCCCCCACAATCCCAAGACCAGAGGCCTTCTCCAAGCCCTCTCTTTCTTCCCCAATCTTCTTCCCCTCCAGAAGAACCGCCGCCTTTGCCTATGGCTTCTCGTTCGTCTGCATCGCTTGCACTTTCTTCCTGGTTTTCAACCCATCTGGCTACTCTTTCCCTTTCGGCTTCAAGAACATCTTCAAAACCCCTTTTGAACTTTCCCGGGAAACCCCATTTTCCTGGAAAGCTGAATATTTCCCGCGTTTGGATGAGAGTGTAGGTTTGCAGTCTGATATGGCTAGTGCCCAACTGCCGAACCCAATTCTTTTCTTGCCCCAAGAATCCAATCAGACTTTGTCCCAGAACACTACGATTCAAGTAACTGGGAAAATGGGATTTTCCGGGAAACTGGAGGGTTCAAGAAAAAATGAATCTTTTGCTAAAGATTCTGATGGGGTTTTGGGGCAAGCAGTGGCATTGACCTCGAAAGAGAGTGATGATGCTAGTGAGGCATTGTCTGAATCTGAGAAGCAGAGCAATCAAACTCAGCCCGGTTATGAATTTTCGAGGGATGATTGTGACCTGTTTGATGGAATGTGGGTGAGAGATGATTCGTATCCGCTTTATGCTCCGGGTTCTTGTCCTCTTATTGATGAATCCTTCAATTGTTTTCTTAATAAGAGGCCGGATAATCGCTACGAAAAGTATAGATGGCAACCCAAACATTGCAATCTTCCAAGCTTGTTGGATGGTAAGAACATGTTGGGACTATTGAGAGGAAAGCGACTGGTTTTTGTTGGGGATTCTCTTAATAGGAATATGTGGGAGTCTTTGCTTTGTCTTCTTCGAAATTCAGTGGATGATAAAAGTGGAGTTGTTGAAGCGTTGGGTAGGAGCGAATTTCGTTCAGAGGGATCTTACTCTTTCGTATTCAAGGAGTACAATTGCTCAGTGGAATTCTTTCGATCGCCATTTCTAGTTCAAGAATGGGAGATGGCAGATGCAAATGGATCAATAAAGGAAACGCTTCGCCTTGATTTGATTGAGAGATCCTCCGATAAGTACACAAAACTGCAGATGTTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAGAGGAATCTTGCAGGAAGGGTTACTATCAAGAGGGTAGCTACATCTACGATGTATTGAACGTTAAAGAGGCATTTCGGAAAGCTTTAACAACATGGGCAAGATGGGTGGATACCAACATAAATCCTGAGAAAACTATTGTTTTCTTCCGGGGCTTTTCACCTAATCACTTTAGAGGTGGAAGATGGAATTCGGGAGGGCAATGCCATGATCTAATAGAGCCACTTGCAAAGGAGACAAATAGAGGAAAATATGCATCAAAATTGAGAATATTTGACTCAGTAATCACTGGAATGAAGACGCCAATATTCTATCTAAACATTACGAGAATGACTGATTTCAGGAGGGATGCTCATCCATCGATTTACAGAAAGCAGAATTTTACAGAGGAGGAGCGGAAATCGCCTTTGAGACACCAGGATTGCAGTCACTGGTGCCTCCCTGGTGTTCCTGATACATGGAATGAGCTTCTATATGCTCAGCTCGTTCGACATAACCAGAAGCAACAACAACAGCAGCGTCAAGACCCACAGCGACAGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

569

Amino Acids

65.32

Weight (kDa)

8.55

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 203 - 255 3.4e-22 PMR5 N terminal Domain
PC-Esterase PF13839 260 - 546 6.6e-83 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1593
AccBSI CCGCTC 1 cut(s) 1555
AccII CGCG 1 cut(s) 295
AciI CCGC 6 cut(s) 5, 129, 132, 293, 653, 1555
AclI AACGTT 1 cut(s) 1221
AclWI GGATC 3 cut(s) 955, 1051, 1077
AcsI RAATTY 6 cut(s) 593, 883, 932, 989, 1348, 1537
AcyI GRCGYC 1 cut(s) 1460
AfaI GTAC 2 cut(s) 974, 1097
AfiI CCNNNNNNNGG 1 cut(s) 298
AflIII ACRYGT 1 cut(s) 792
AjnI CCWGG 4 cut(s) 180, 272, 1575, 1600
AluBI AGCT 6 cut(s) 281, 774, 1200, 1244, 1627, 1642
AluI AGCT 6 cut(s) 281, 774, 1200, 1244, 1627, 1642
Alw26I GTCTC 2 cut(s) 1388, 1564
AlwI GGATC 3 cut(s) 955, 1051, 1077
AlwNI CAGNNNCTG 1 cut(s) 1590
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 2 cut(s) 75, 716
ApeKI GCWGC 1 cut(s) 1675
ApoI RAATTY 6 cut(s) 593, 883, 932, 989, 1348, 1537
Asp700I GAANNNNTTC 3 cut(s) 230, 993, 1059
AsuC2I CCSGG 6 cut(s) 256, 257, 436, 585, 666, 1310
AsuHPI GGTGA 2 cut(s) 646, 1311
AsuII TTCGAA 1 cut(s) 880
AvaI CYCGRG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 1593
BbsI GAAGAC 2 cut(s) 866, 1463
BbvI GCAGC 1 cut(s) 1687
BccI CCATC 9 cut(s) 202, 479, 617, 738, 776, 1021, 1257, 1338, 1525
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 4 cut(s) 182, 274, 1577, 1602
BciVI GTATCC 2 cut(s) 660, 1264
BcnI CCSGG 6 cut(s) 256, 257, 436, 585, 666, 1310
BcoDI GTCTC 2 cut(s) 1388, 1564
BfaI CTAG 4 cut(s) 333, 537, 1010, 1705
BfmI CTRYAG 1 cut(s) 1106
BfuI GTATCC 2 cut(s) 660, 1264
BisI GCNGC 2 cut(s) 132, 1676
BlpI GCTNAGC 1 cut(s) 1638
BlsI GCNGC 2 cut(s) 133, 1677
BmeT110I CYCGRG 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 1595
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 5 cut(s) 171, 523, 1024, 1424, 1498
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 2 cut(s) 866, 1463
BplI GAGNNNNNCTC 6 cut(s) 65, 97, 1070, 1102, 1495, 1527
BpmI CTGGAG 2 cut(s) 103, 464
Bpu1102I GCTNAGC 1 cut(s) 1638
Bpu14I TTCGAA 1 cut(s) 880
BpuMI CCSGG 6 cut(s) 256, 257, 436, 585, 666, 1310
Bsa29I ATCGAT 1 cut(s) 1520
BsaBI GATNNNNATC 2 cut(s) 648, 1512
BsaHI GRCGYC 1 cut(s) 1460
BsaJI CCNNGG 3 cut(s) 255, 1309, 1600
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 6 cut(s) 421, 447, 825, 1141, 1453, 1595
Bse3DI GCAATG 2 cut(s) 757, 1367
Bse8I GATNNNNATC 2 cut(s) 648, 1512
BseBI CCWGG 4 cut(s) 182, 274, 1577, 1602
BseCI ATCGAT 1 cut(s) 1520
BseDI CCNNGG 3 cut(s) 255, 1309, 1600
BseGI GGATG 6 cut(s) 307, 610, 787, 898, 1513, 1513
BseJI GATNNNNATC 2 cut(s) 648, 1512
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 757, 1367
BseMII CTCAG 6 cut(s) 549, 593, 996, 1278, 1452, 1652
BseNI ACTGG 6 cut(s) 421, 447, 825, 1141, 1453, 1595
BseRI GAGGAG 2 cut(s) 1562, 1565
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 1 cut(s) 1687
Bsh1236I CGCG 1 cut(s) 295
Bsh1285I CGRYCG 1 cut(s) 1001
BshFI GGCC 2 cut(s) 77, 718
BshNI GGYRCC 1 cut(s) 1593
BshVI ATCGAT 1 cut(s) 1520
BsiEI CGRYCG 1 cut(s) 1001
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 6 cut(s) 256, 435, 585, 665, 719, 1309
BslFI GGGAC 2 cut(s) 376, 813
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 2 cut(s) 1388, 1564
BsmFI GGGAC 2 cut(s) 376, 813
BsnI GGCC 2 cut(s) 77, 718
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 880
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 5 cut(s) 947, 998, 1043, 1082, 1369
Bsp1720I GCTNAGC 1 cut(s) 1638
BspACI CCGC 6 cut(s) 5, 129, 132, 293, 653, 1555
BspANI GGCC 2 cut(s) 77, 718
BspCNI CTCAG 6 cut(s) 550, 592, 995, 1279, 1451, 1651
BspDI ATCGAT 1 cut(s) 1520
BspFNI CGCG 1 cut(s) 295
BspHI TCATGA 1 cut(s) 1146
BspLI GGNNCC 1 cut(s) 1595
BspMAI CTGCAG 1 cut(s) 1110
BspPI GGATC 3 cut(s) 955, 1051, 1077
BspT104I TTCGAA 1 cut(s) 880
BspT107I GGYRCC 1 cut(s) 1593
BsrBI CCGCTC 1 cut(s) 1555
BsrDI GCAATG 2 cut(s) 757, 1367
BsrI ACTGG 6 cut(s) 421, 447, 825, 1141, 1453, 1595
BssECI CCNNGG 3 cut(s) 255, 1309, 1600
BssMI GATC 5 cut(s) 947, 998, 1043, 1082, 1369
BssNI GRCGYC 1 cut(s) 1460
Bst2UI CCWGG 4 cut(s) 182, 274, 1577, 1602
BstACI GRCGYC 1 cut(s) 1460
BstAPI GCANNNNNTGC 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 2 cut(s) 169, 501
BstDEI CTNAG 6 cut(s) 558, 579, 982, 1287, 1438, 1638
BstF5I GGATG 6 cut(s) 307, 610, 787, 898, 1513, 1513
BstFNI CGCG 1 cut(s) 295
BstKTI GATC 5 cut(s) 950, 1001, 1046, 1085, 1372
BstMAI GTCTC 2 cut(s) 1388, 1564
BstMBI GATC 5 cut(s) 947, 998, 1043, 1082, 1369
BstMCI CGRYCG 1 cut(s) 1001
BstMWI GCNNNNNNNGC 4 cut(s) 137, 168, 542, 1561
BstNI CCWGG 4 cut(s) 182, 274, 1577, 1602
BstNSI RCATGY 1 cut(s) 796
BstSFI CTRYAG 1 cut(s) 1106
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 1687
BstV2I GAAGAC 2 cut(s) 866, 1463
BstX2I RGATCY 2 cut(s) 947, 1082
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 2 cut(s) 947, 1082
Bsu15I ATCGAT 1 cut(s) 1520
BsuI GTATCC 2 cut(s) 660, 1264
BsuRI GGCC 2 cut(s) 77, 718
BsuTUI ATCGAT 1 cut(s) 1520
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 6 cut(s) 307, 610, 787, 898, 1513, 1513
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 6 cut(s) 510, 894, 990, 1134, 1446, 1588
Cac8I GCNNGC 2 cut(s) 169, 501
CaiI CAGNNNCTG 1 cut(s) 1590
CciI TCATGA 1 cut(s) 1146
Cfr9I CCCGGG 1 cut(s) 255
ClaI ATCGAT 1 cut(s) 1520
CseI GACGC 2 cut(s) 1468, 1667
Csp6I GTAC 2 cut(s) 973, 1096
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
CviAII CATG 5 cut(s) 793, 1147, 1254, 1367, 1617
CviQI GTAC 2 cut(s) 973, 1096
DdeI CTNAG 6 cut(s) 558, 579, 982, 1287, 1438, 1638
DpnI GATC 5 cut(s) 949, 1000, 1045, 1084, 1371
DpnII GATC 5 cut(s) 947, 998, 1043, 1082, 1369
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 255
EcoRI GAATTC 2 cut(s) 989, 1348
EcoRII CCWGG 4 cut(s) 180, 272, 1575, 1600
EcoT22I ATGCAT 1 cut(s) 1417
FaeI CATG 5 cut(s) 796, 1150, 1257, 1370, 1620
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 376, 813
FatI CATG 5 cut(s) 792, 1146, 1253, 1366, 1616
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 132, 1676
FokI GGATG 6 cut(s) 314, 617, 794, 905, 1500, 1520
Fsp4HI GCNGC 2 cut(s) 132, 1676
FspBI CTAG 4 cut(s) 333, 537, 1010, 1705
GluI GCNGC 2 cut(s) 132, 1676
GsuI CTGGAG 2 cut(s) 103, 464
HaeIII GGCC 2 cut(s) 77, 718
HapII CCGG 6 cut(s) 256, 435, 585, 665, 719, 1309
HgaI GACGC 2 cut(s) 1468, 1667
Hin1I GRCGYC 1 cut(s) 1460
Hin1II CATG 5 cut(s) 796, 1150, 1257, 1370, 1620
HindIII AAGCTT 2 cut(s) 772, 1242
HpaII CCGG 6 cut(s) 256, 435, 585, 665, 719, 1309
HphI GGTGA 2 cut(s) 646, 1311
Hpy166II GTNNAC 2 cut(s) 1098, 1142
Hpy188I TCNGA 8 cut(s) 325, 384, 484, 553, 559, 943, 1090, 1239
Hpy8I GTNNAC 2 cut(s) 1098, 1142
HpyAV CCTTC 3 cut(s) 88, 703, 1171
HpyCH4IV ACGT 1 cut(s) 1221
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 168, 542, 1561
HpyF3I CTNAG 6 cut(s) 558, 579, 982, 1287, 1438, 1638
HpySE526I ACGT 1 cut(s) 1221
Hsp92I GRCGYC 1 cut(s) 1460
Hsp92II CATG 5 cut(s) 796, 1150, 1257, 1370, 1620
Kzo9I GATC 5 cut(s) 947, 998, 1043, 1082, 1369
LmnI GCTCC 3 cut(s) 667, 927, 1552
Lsp1109I GCAGC 1 cut(s) 1687
LweI GCATC 5 cut(s) 171, 523, 1024, 1424, 1498
MaeI CTAG 4 cut(s) 333, 537, 1010, 1705
MaeII ACGT 1 cut(s) 1221
MaeIII GTNAC 5 cut(s) 412, 611, 1132, 1181, 1586
MalI GATC 5 cut(s) 949, 1000, 1045, 1084, 1371
MbiI CCGCTC 1 cut(s) 1555
MboI GATC 5 cut(s) 947, 998, 1043, 1082, 1369
MfeI CAATTG 2 cut(s) 697, 976
MflI RGATCY 2 cut(s) 947, 1082
MhlI GDGCHC 1 cut(s) 340
MlyI GAGTC 3 cut(s) 869, 1137, 1430
MmeI TCCRAC 1 cut(s) 759
Mph1103I ATGCAT 1 cut(s) 1417
MroXI GAANNNNTTC 3 cut(s) 230, 993, 1059
MseI TTAA 4 cut(s) 708, 843, 1224, 1247
MslI CAYNNNNRTG 1 cut(s) 1451
MspI CCGG 6 cut(s) 256, 435, 585, 665, 719, 1309
MunI CAATTG 2 cut(s) 697, 976
MvaI CCWGG 4 cut(s) 182, 274, 1577, 1602
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 4 cut(s) 137, 168, 542, 1561
NciI CCSGG 6 cut(s) 256, 257, 436, 585, 666, 1310
NdeII GATC 5 cut(s) 947, 998, 1043, 1082, 1369
NlaIII CATG 5 cut(s) 796, 1150, 1257, 1370, 1620
NlaIV GGNNCC 1 cut(s) 1595
NmuCI GTSAC 3 cut(s) 611, 1132, 1586
NsiI ATGCAT 1 cut(s) 1417
NspI RCATGY 1 cut(s) 796
NspV TTCGAA 1 cut(s) 880
PagI TCATGA 1 cut(s) 1146
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 792
PdmI GAANNNNTTC 3 cut(s) 230, 993, 1059
PfeI GAWTC 9 cut(s) 374, 406, 464, 479, 554, 644, 689, 836, 1166
PflFI GACNNNGTC 1 cut(s) 388
PfoI TCCNGGA 2 cut(s) 272, 434
PkrI GCNGC 2 cut(s) 133, 1677
Ple19I CGATCG 1 cut(s) 1001
PleI GAGTC 3 cut(s) 868, 1137, 1430
PpsI GAGTC 3 cut(s) 868, 1137, 1430
PscI ACATGT 1 cut(s) 792
Psp1406I AACGTT 1 cut(s) 1221
Psp6I CCWGG 4 cut(s) 180, 272, 1575, 1600
PspGI CCWGG 4 cut(s) 180, 272, 1575, 1600
PspN4I GGNNCC 1 cut(s) 1595
PstI CTGCAG 1 cut(s) 1110
PstNI CAGNNNCTG 1 cut(s) 1590
PsuI RGATCY 2 cut(s) 947, 1082
PsyI GACNNNGTC 1 cut(s) 388
PvuI CGATCG 1 cut(s) 1001
RsaI GTAC 2 cut(s) 974, 1097
RsaNI GTAC 2 cut(s) 973, 1096
RseI CAYNNNNRTG 1 cut(s) 1451
SaqAI TTAA 4 cut(s) 708, 843, 1224, 1247
SatI GCNGC 2 cut(s) 132, 1676
Sau3AI GATC 5 cut(s) 947, 998, 1043, 1082, 1369
SchI GAGTC 3 cut(s) 869, 1137, 1430
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 5 cut(s) 171, 523, 1024, 1424, 1498
SfcI CTRYAG 1 cut(s) 1106
SfuI TTCGAA 1 cut(s) 880
SmaI CCCGGG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1451
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 6 cut(s) 5, 129, 132, 293, 653, 1555
SspI AATATT 3 cut(s) 287, 1431, 1467
SspMI CTAG 4 cut(s) 333, 537, 1010, 1705
StuI AGGCCT 1 cut(s) 77
TaiI ACGT 1 cut(s) 1224
TaqI TCGA 6 cut(s) 518, 599, 880, 997, 1520, 1648
TatI WGTACW 2 cut(s) 972, 1095
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 9 cut(s) 374, 406, 464, 479, 554, 644, 689, 836, 1166
Tru1I TTAA 4 cut(s) 708, 843, 1224, 1247
Tru9I TTAA 4 cut(s) 708, 843, 1224, 1247
TscAI CASTG 6 cut(s) 510, 894, 990, 1141, 1453, 1595
TseFI GTSAC 3 cut(s) 611, 1132, 1586
TseI GCWGC 1 cut(s) 1675
Tsp45I GTSAC 3 cut(s) 611, 1132, 1586
TspDTI ATGAA 4 cut(s) 477, 606, 702, 1469
TspMI CCCGGG 1 cut(s) 255
TspRI CASTG 6 cut(s) 510, 894, 990, 1141, 1453, 1595
Tth111I GACNNNGTC 1 cut(s) 388
XapI RAATTY 6 cut(s) 593, 883, 932, 989, 1348, 1537
XceI RCATGY 1 cut(s) 796
XmaI CCCGGG 1 cut(s) 255
XmnI GAANNNNTTC 3 cut(s) 230, 993, 1059
XspI CTAG 4 cut(s) 333, 537, 1010, 1705
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.