Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000874

Physical Location & Seq

Genomic Coordinates Forward (+)

18795730 .. 18798054

Gene Structure 2325 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1131300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGAACCACCTATCACTCTCAGCCTCAAAGCACCTCCTTAAACCCCAACCCTCCAAAACCCAATCCTCCGTCGTTCTCCTCCTCCTCAACCACCAATTCTCCACCGATTCCGATCCCAATCCCAACCCCAAACCCAGCAAACAACCCCACGAAGATGACTCGCTAGTAACCCAAGTCGTCCAACTCCTCGAATCCGATGAAAAAGACTGGAACTTGGACCAGCTCCGCCACCTCCTCTTCTCCGACTCCGCCGCCGCTCCTTCTCCTCGCTCTCTCTTCCGCATCACTCGCCGCCTCAACACTTCCTCCAAAGCCCTCAAGTTCTTCGACTACGTTTCCGAGAATGTGGCAACCTCACCGGACTCGGCGGCGGCGGCGGCCTCGCTCTCTTCATCATTCCAGGCCGTTCTCGAGCTCGCTGAGCGAGAACCCTACTCGCAAACTAGGCTTTTCGACATGTATAAGATGGCGAAAGAGCGGAACATTCCGGTTAATATGGGCGCCGCTGTTCTGCTAGTTCGATCGCTGGGCTTTGCTGGTATGGTGGATGAGGCGGTCAACGTGTATAACGGTCTGGACCCCGGTTTGAAGAATACCCATCTTCGCAATTGGGTGATTAGTGTGCTGTTGAAATGGGGACGGGTCGATGATGCACTGAAGGTGCTCGACAAAATGTTTGATCCAAATGCAGAGTTCCGGGTTGACAGTGTTACTGCTGATATTGTGCTTTCCTCTTTGCTGAAGAGAGAGTGGCCCGGGAGGAGCGTCAGTGAGGAGGACATTGTGGGTTTGGTGCAGAAATTTGGGGAGCATGGTGTGTTTCCTGATAGTTTGAAACTTACGAAATTGATCACGTCGTTGTGTAGGAACAGGAACACTAGTCGGGCTTGGGACGTTTTATATTACATTATTAATTCTGGTGGTGCTGTAGAAGCTGCTTGTTGCAATGCGCTTTTGACAGGTTTGGGAAGAGTTAAAGATTTTAAGAGGATGAATGAGCTTATGGTAAAGATGAAAGAAATGGACATCCCTCCCAATGTTGTAACTTACGGTATTGTCATTAACTGTTTGTGCAAGTCTAGGAGGGTGGATGAGGCCTTGGAGTTGTTTGAAAGGATAAGTGGAGGAGGAGAGAAACCTGATGGGATTTCGGCTGAACCCGATGTGATCATTTTTAACACTCTGATTGATGGACTTTGTAAAGTGGGAAGGCAAGAAGAAGGATTACGTCTGATGGAAAAAATGAGATTGCAAGATGGCTGTGCACCTAATACTGTTACCTACAATAGTTTGATTGACGGTTTCAACAAGGTTGGGGACATCGAGAGGGGTCGTGAGCTCTATGATCAAATGAAGGAGGAAGGTATACCGCCAAGTGTAGTTACTCTCAATACTTTGGTTGATGGTCTCTGTAAACATGGGAGGCTCAACAGCGCACTGGAGTTCCTTAATGAAATGCAGAGGGATGGTATAAAAGGCAATGTCACAACGTACACGATGCTAATCACTTCCTTTTGTAATGTAAACAATATTGGCATGGCAATGGAACTGTTTGAGCAAATGTTAAGTTCTGGATGCTCCACAGATGCAAAAGTTTACTACCGCTTGATCTCTGGTTTAAGCCAAGCCAGAAGGATGGATGATGCCAACATTGTTGTATCAAAGTTGAAGGAGGCCGGGTTCTCCCTGGATGTCATTGCCTTCAATGTTTTGATCAACGGGTTCTGCAAGACAAAAAAACTCGAGAAGGTGCATGAGATGATCCAGGAAATGGAGACAGCTGGAGTAAAGCCTGATAGCATCACATACAACACGCTGATTTCATATTTGTGCTCAGCTGGGGAACTAACAACTGCTCATAGAGTCCTGAGCAAGATGATAAATGAGGGCCTTGTTCCAACTGTTTTCACTTTTGGATCATTGATTCATGCATATTGTTTGAAGGGCGATACATACAAAGCCATGAAGATCTTCAGAGACATGGGTTCTAAGTGGAGTGCTCCTCCCAACACAGTAGTATACAACATCTTAATAGATTCTTTATGCAAGAATAATGAAGTGGACGTCGCATTTTCGTTGATGGATAGTATGAAAAATAAGGGAGTGAGACCTAATACCTTGACATTCAATGCCATGTTCAAAGGTCTTAAGGAGAATAATTTGCTGGAGAAAGCATTTAAATTAATGGATCAAATGATTGAACAGGCATGTAATCCGGATTATATAACCATGGAGATCCTGACCGAATGGCTTTCTGCTGTTGGTGAAACCGAAAAGTTGAGAAGGTTTGTGCAAGGATATGAAGTTGCTGCTTCCACTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

775

Amino Acids

86.22

Weight (kDa)

6.01

Isoelectric Point (pI)

31.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	313 - 359	1.1e-10	PPR repeat family
PPR_3	PF13812	314 - 356	2.4e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	342 - 372	5.9e-11	PPR repeat
PPR_2	PF13041	345 - 381	2.6e-07	PPR repeat family
PPR	PF01535	348 - 373	3.6e-06	PPR repeat
PPR_3	PF13812	386 - 433	4.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	386 - 416	2.3e-09	PPR repeat
PPR_2	PF13041	387 - 436	2.7e-14	PPR repeat family
PPR_1	PF12854	419 - 452	8.1e-09	PPR repeat
PPR_2	PF13041	423 - 472	3.1e-16	PPR repeat family
TPR_24	PF23276	424 - 520	1.3e-08	Fungal tetratrico peptide repeats
PPR	PF01535	426 - 456	1.1e-07	PPR repeat
PPR_1	PF12854	455 - 487	7.5e-13	PPR repeat
PPR_2	PF13041	458 - 507	5.6e-17	PPR repeat family
PPR	PF01535	461 - 491	6.5e-08	PPR repeat
TPR_24	PF23276	473 - 586	8.2e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	483 - 527	1.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	490 - 521	5.9e-07	PPR repeat
PPR_2	PF13041	494 - 541	3.4e-09	PPR repeat family
PPR_long	PF17177	510 - 628	4.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	534 - 577	5e-07	PPR repeat family
PPR_3	PF13812	552 - 609	1.3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	564 - 611	4.3e-15	PPR repeat family
PPR	PF01535	567 - 596	7.1e-06	PPR repeat
PPR_long	PF17177	578 - 663	1.2e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	588 - 645	7.1e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	595 - 626	3e-10	PPR repeat
PPR_2	PF13041	598 - 646	1.1e-13	PPR repeat family
PPR_3	PF13812	621 - 680	9.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	630 - 661	9.5e-09	PPR repeat
PPR_long	PF17177	652 - 765	4.2e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	668 - 698	6.5e-11	PPR repeat
PPR_2	PF13041	669 - 716	1.4e-17	PPR repeat family
TPR_24	PF23276	671 - 763	2.1e-06	Fungal tetratrico peptide repeats
PPR	PF01535	672 - 702	5e-07	PPR repeat
PPR_2	PF13041	709 - 747	3.3e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000874)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61520 AT5G28370 AT5G28460
fragaria_vesca	FvH4_5g03011 FvH4_5g03011 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_7g15360
malus_domestica	MD01G1071400.v1.1 MD07G1131300.v1.1
prunus_persica	Prupe.2G177500_v2.0.a1
pyrus_communis	pycom01g10040 pycom04g02360 pycom07g12830
rosa_chinensis	RchiOBHm_Chr1g0357681 RchiOBHm_Chr3g0468501 RchiOBHm_Chr5g0017031 RchiOBHm_Chr7g0228211 RchiOBHm_Chr7g0228281 RchiOBHm_Chr7g0228291
rosa_laevigata	RLG00000001577 RLG00000005833 RLG00000009015 RLG00000009124 RLG00000012161 RLG00000028007 RLG00000028484
rosa_multiflora	Rmu_sc0001062.1_g000004 Rmu_sc0001626.1_g000014 Rmu_sc0001626.1_g000015 Rmu_sc0002582.1_g000009 Rmu_sc0003490.1_g000025 Rmu_sc0003490.1_g000026 Rmu_sc0004842.1_g000011 Rmu_sc0004842.1_g000012 Rmu_sc0004842.1_g000020 Rmu_ssc0000425.1_g000002
rosa_roxburghii	Rroxscaffold_3G00231300 Rroxscaffold_4G00298600 Rroxscaffold_6G00429570
rosa_rugosa	Rorug01G0257700 Rorug02G0051500 Rorug02G0572600 Rorug07G0246900.1 Rorug07G0247000 Rorug07G0247000
rosa_samantha	Rh1AG271700 Rh1BG238800 Rh1CG255600 Rh1DG267200 Rh3AG143700 Rh3BG166200 Rh3BG166300 Rh5AG043600 Rh7AG400900 Rh7AG401200 Rh7BG382400 Rh7CG420000 Rh7DG396900 Rh7DG397300
rosa_wichuraiana	Rw1G024230 Rw7G033300 Rw7G033330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2067
AccB1I	GGYRCC	1 cut(s)	500
AccB7I	CCANNNNNTGG	1 cut(s)	1771
AccBSI	CCGCTC	2 cut(s)	257, 478
AccI	GTMKAC	2 cut(s)	1366, 2019
AccIII	TCCGGA	1 cut(s)	2215
AclWI	GGATC	6 cut(s)	107, 674, 1756, 1924, 2196, 2230
AcsI	RAATTY	1 cut(s)	800
AcuI	CTGAAG	3 cut(s)	677, 761, 1957
AcyI	GRCGYC	2 cut(s)	501, 2064
AfaI	GTAC	1 cut(s)	1493
AfiI	CCNNNNNNNGG	1 cut(s)	1771
AflII	CTTAAG	1 cut(s)	2147
AflIII	ACRYGT	2 cut(s)	456, 561
AhlI	ACTAGT	1 cut(s)	878
AjiI	CACGTC	1 cut(s)	855
AjnI	CCWGG	3 cut(s)	399, 1686, 1764
AloI	GAACNNNNNNTCC	2 cut(s)	1664, 1696
AluBI	AGCT	7 cut(s)	223, 415, 935, 1000, 1339, 1781, 1838
AluI	AGCT	7 cut(s)	223, 415, 935, 1000, 1339, 1781, 1838
Alw21I	GWGCWC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
Alw26I	GTCTC	4 cut(s)	1412, 1769, 1971, 2101
Alw44I	GTGCAC	1 cut(s)	1263
AlwI	GGATC	6 cut(s)	107, 674, 1756, 1924, 2196, 2230
Ama87I	CYCGRG	3 cut(s)	410, 755, 1742
Aor13HI	TCCGGA	1 cut(s)	2215
AoxI	GGCC	6 cut(s)	378, 402, 752, 1095, 1674, 1888
ApaLI	GTGCAC	1 cut(s)	1263
ApeKI	GCWGC	2 cut(s)	935, 2309
ApoI	RAATTY	1 cut(s)	800
ArsI	GACNNNNNNTTYG	2 cut(s)	659, 691
AseI	ATTAAT	2 cut(s)	912, 2183
Asp700I	GAANNNNTTC	1 cut(s)	1970
AspLEI	GCGC	3 cut(s)	503, 952, 1436
AspS9I	GGNCC	4 cut(s)	217, 577, 753, 1888
AsuC2I	CCSGG	5 cut(s)	582, 698, 756, 757, 1678
AsuHPI	GGTGA	3 cut(s)	348, 625, 2276
AvaI	CYCGRG	3 cut(s)	410, 755, 1742
AvaII	GGWCC	2 cut(s)	217, 577
BaeGI	GKGCMC	1 cut(s)	1267
BanI	GGYRCC	1 cut(s)	500
BanII	GRGCYC	2 cut(s)	417, 1341
BarI	GAAGNNNNNNTAC	2 cut(s)	1209, 1241
Bbv12I	GWGCWC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
BbvI	GCAGC	2 cut(s)	922, 2296
BceAI	ACGGC	1 cut(s)	389
BciT130I	CCWGG	3 cut(s)	401, 1688, 1766
BclI	TGATCA	4 cut(s)	849, 1167, 1345, 1713
BcnI	CCSGG	5 cut(s)	582, 698, 756, 757, 1678
BcoDI	GTCTC	4 cut(s)	1412, 1769, 1971, 2101
BcuI	ACTAGT	1 cut(s)	878
BfaI	CTAG	5 cut(s)	164, 444, 515, 879, 1080
BfmI	CTRYAG	1 cut(s)	927
BfoI	RGCGCY	1 cut(s)	504
BfrI	CTTAAG	1 cut(s)	2147
BglII	AGATCT	1 cut(s)	1968
BlpI	GCTNAGC	2 cut(s)	420, 1834
Bme1390I	CCNGG	8 cut(s)	401, 582, 698, 756, 757, 1678, 1688, 1766
Bme18I	GGWCC	2 cut(s)	217, 577
BmeT110I	CYCGRG	3 cut(s)	410, 755, 1742
BmgBI	CACGTC	1 cut(s)	855
BmgT120I	GGNCC	4 cut(s)	217, 577, 753, 1888
BmiI	GGNNCC	2 cut(s)	502, 579
BmrFI	CCNGG	8 cut(s)	401, 582, 698, 756, 757, 1678, 1688, 1766
BmsI	GCATC	7 cut(s)	291, 640, 1488, 1565, 1576, 1633, 1809
BplI	GAGNNNNNCTC	2 cut(s)	1987, 2019
BpmI	CTGGAG	3 cut(s)	1460, 1803, 2186
Bpu10I	CCTNAGC	1 cut(s)	1868
Bpu1102I	GCTNAGC	2 cut(s)	420, 1834
BpuEI	CTTGAG	1 cut(s)	302
BpuMI	CCSGG	5 cut(s)	582, 698, 756, 757, 1678
BsaBI	GATNNNNATC	3 cut(s)	111, 117, 1502
BsaHI	GRCGYC	2 cut(s)	501, 2064
BsaI	GGTCTC	2 cut(s)	1412, 2101
BsaJI	CCNNGG	5 cut(s)	580, 755, 1098, 1686, 2229
BsaWI	WCCGGW	3 cut(s)	358, 487, 2215
BsaXI	ACNNNNNCTCC	8 cut(s)	83, 113, 290, 320, 754, 784, 1664, 1694
Bsc4I	CCNNNNNNNGG	1 cut(s)	1771
Bse1I	ACTGG	2 cut(s)	212, 1443
Bse3DI	GCAATG	4 cut(s)	952, 1486, 1548, 1695
Bse8I	GATNNNNATC	3 cut(s)	111, 117, 1502
BseAI	TCCGGA	1 cut(s)	2215
BseBI	CCWGG	3 cut(s)	401, 1688, 1766
BseDI	CCNNGG	5 cut(s)	580, 755, 1098, 1686, 2229
BseGI	GGATG	9 cut(s)	553, 996, 1026, 1096, 1471, 1580, 1641, 1645, 1696
BseJI	GATNNNNATC	3 cut(s)	111, 117, 1502
BseLI	CCNNNNNNNGG	1 cut(s)	1771
BseMI	GCAATG	4 cut(s)	952, 1486, 1548, 1695
BseMII	CTCAG	4 cut(s)	33, 411, 1848, 1859
BseNI	ACTGG	2 cut(s)	212, 1443
BseSI	GKGCMC	1 cut(s)	1267
BseXI	GCAGC	2 cut(s)	922, 2296
BseYI	CCCAGC	3 cut(s)	134, 526, 1838
BsgI	GTGCAG	1 cut(s)	815
Bsh1285I	CGRYCG	1 cut(s)	524
BshFI	GGCC	6 cut(s)	380, 404, 754, 1097, 1676, 1890
BshNI	GGYRCC	1 cut(s)	500
BsiEI	CGRYCG	1 cut(s)	524
BsiHKAI	GWGCWC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
BsiHKCI	CYCGRG	3 cut(s)	410, 755, 1742
BsiSI	CCGG	7 cut(s)	359, 488, 582, 697, 756, 1677, 2216
BslFI	GGGAC	3 cut(s)	651, 905, 1331
BslI	CCNNNNNNNGG	1 cut(s)	1771
BsmAI	GTCTC	4 cut(s)	1412, 1769, 1971, 2101
BsmFI	GGGAC	3 cut(s)	651, 905, 1331
BsnI	GGCC	6 cut(s)	380, 404, 754, 1097, 1676, 1890
Bso31I	GGTCTC	2 cut(s)	1412, 2101
BsoBI	CYCGRG	3 cut(s)	410, 755, 1742
Bsp1286I	GDGCHC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
Bsp13I	TCCGGA	1 cut(s)	2215
Bsp1720I	GCTNAGC	2 cut(s)	420, 1834
Bsp19I	CCATGG	1 cut(s)	2229
BspANI	GGCC	6 cut(s)	380, 404, 754, 1097, 1676, 1890
BspCNI	CTCAG	4 cut(s)	32, 412, 1847, 1860
BspEI	TCCGGA	1 cut(s)	2215
BspLI	GGNNCC	2 cut(s)	502, 579
BspPI	GGATC	6 cut(s)	107, 674, 1756, 1924, 2196, 2230
BspT107I	GGYRCC	1 cut(s)	500
BspTI	CTTAAG	1 cut(s)	2147
BspTNI	GGTCTC	2 cut(s)	1412, 2101
BsrBI	CCGCTC	2 cut(s)	257, 478
BsrDI	GCAATG	4 cut(s)	952, 1486, 1548, 1695
BsrI	ACTGG	2 cut(s)	212, 1443
BssECI	CCNNGG	5 cut(s)	580, 755, 1098, 1686, 2229
BssNAI	GTATAC	2 cut(s)	1367, 2020
BssNI	GRCGYC	2 cut(s)	501, 2064
BssT1I	CCWWGG	2 cut(s)	1098, 2229
Bst1107I	GTATAC	2 cut(s)	1367, 2020
Bst2UI	CCWGG	3 cut(s)	401, 1688, 1766
Bst4CI	ACNGT	9 cut(s)	572, 707, 1052, 1067, 1276, 1301, 1551, 1903, 2014
Bst6I	CTCTTC	5 cut(s)	242, 281, 394, 737, 964
BstACI	GRCGYC	2 cut(s)	501, 2064
BstAFI	CTTAAG	1 cut(s)	2147
BstC8I	GCNNGC	1 cut(s)	417
BstDEI	CTNAG	5 cut(s)	19, 420, 1834, 1868, 1989
BstDSI	CCRYGG	1 cut(s)	2229
BstF5I	GGATG	9 cut(s)	553, 996, 1026, 1096, 1471, 1580, 1641, 1645, 1696
BstH2I	RGCGCY	1 cut(s)	504
BstHHI	GCGC	3 cut(s)	503, 952, 1436
BstMAI	GTCTC	4 cut(s)	1412, 1769, 1971, 2101
BstMCI	CGRYCG	1 cut(s)	524
BstMWI	GCNNNNNNNGC	7 cut(s)	288, 374, 377, 421, 445, 932, 2318
BstNI	CCWGG	3 cut(s)	401, 1688, 1766
BstNSI	RCATGY	2 cut(s)	460, 2211
BstSCI	CCNGG	8 cut(s)	399, 580, 696, 754, 755, 1676, 1686, 1764
BstSFI	CTRYAG	1 cut(s)	927
BstSLI	GKGCMC	1 cut(s)	1267
BstV1I	GCAGC	2 cut(s)	922, 2296
BstX2I	RGATCY	2 cut(s)	1968, 2235
BstXI	CCANNNNNNTGG	1 cut(s)	1636
BstYI	RGATCY	2 cut(s)	1968, 2235
BstZ17I	GTATAC	2 cut(s)	1367, 2020
BsuRI	GGCC	6 cut(s)	380, 404, 754, 1097, 1676, 1890
BtgI	CCRYGG	1 cut(s)	2229
BtrI	CACGTC	1 cut(s)	855
BtsCI	GGATG	9 cut(s)	553, 996, 1026, 1096, 1471, 1580, 1641, 1645, 1696
BtsI	GCAGTG	1 cut(s)	2316
BtsIMutI	CAGTG	5 cut(s)	653, 712, 775, 1436, 2316
Cac8I	GCNNGC	1 cut(s)	417
CfoI	GCGC	3 cut(s)	503, 952, 1436
Cfr13I	GGNCC	4 cut(s)	217, 577, 753, 1888
Cfr9I	CCCGGG	1 cut(s)	755
CseI	GACGC	1 cut(s)	754
Csp6I	GTAC	1 cut(s)	1492
CviQI	GTAC	1 cut(s)	1492
DdeI	CTNAG	5 cut(s)	19, 420, 1834, 1868, 1989
DinI	GGCGCC	1 cut(s)	502
DraI	TTTAAA	1 cut(s)	2179
Eam1104I	CTCTTC	5 cut(s)	242, 281, 394, 737, 964
EarI	CTCTTC	5 cut(s)	242, 281, 394, 737, 964
EciI	GGCGGA	2 cut(s)	215, 238
Ecl136II	GAGCTC	2 cut(s)	415, 1339
Eco130I	CCWWGG	2 cut(s)	1098, 2229
Eco147I	AGGCCT	1 cut(s)	1097
Eco24I	GRGCYC	2 cut(s)	417, 1341
Eco31I	GGTCTC	2 cut(s)	1412, 2101
Eco47I	GGWCC	2 cut(s)	217, 577
Eco53kI	GAGCTC	2 cut(s)	415, 1339
Eco57I	CTGAAG	3 cut(s)	677, 761, 1957
Eco88I	CYCGRG	3 cut(s)	410, 755, 1742
EcoICRI	GAGCTC	2 cut(s)	415, 1339
EcoO109I	RGGNCCY	1 cut(s)	1888
EcoRII	CCWGG	3 cut(s)	399, 1686, 1764
EcoT14I	CCWWGG	2 cut(s)	1098, 2229
EcoT22I	ATGCAT	1 cut(s)	1933
EcoT38I	GRGCYC	2 cut(s)	417, 1341
EgeI	GGCGCC	1 cut(s)	502
EheI	GGCGCC	1 cut(s)	502
ErhI	CCWWGG	2 cut(s)	1098, 2229
FaqI	GGGAC	3 cut(s)	651, 905, 1331
FbaI	TGATCA	4 cut(s)	849, 1167, 1345, 1713
FblI	GTMKAC	2 cut(s)	1366, 2019
FokI	GGATG	9 cut(s)	560, 1003, 1013, 1103, 1478, 1587, 1648, 1652, 1703
FriOI	GRGCYC	2 cut(s)	417, 1341
FspBI	CTAG	5 cut(s)	164, 444, 515, 879, 1080
GlaI	GCGC	3 cut(s)	502, 951, 1435
GsaI	CCCAGC	3 cut(s)	138, 530, 1842
GsuI	CTGGAG	3 cut(s)	1460, 1803, 2186
HaeII	RGCGCY	1 cut(s)	504
HaeIII	GGCC	6 cut(s)	380, 404, 754, 1097, 1676, 1890
HapII	CCGG	7 cut(s)	359, 488, 582, 697, 756, 1677, 2216
HgaI	GACGC	1 cut(s)	754
HhaI	GCGC	3 cut(s)	503, 952, 1436
Hin1I	GRCGYC	2 cut(s)	501, 2064
Hin6I	GCGC	3 cut(s)	501, 950, 1434
HinP1I	GCGC	3 cut(s)	501, 950, 1434
HincII	GTYRAC	2 cut(s)	559, 703
HindII	GTYRAC	2 cut(s)	559, 703
HinfI	GANTC	8 cut(s)	107, 158, 191, 245, 362, 1863, 1924, 2036
HpaII	CCGG	7 cut(s)	359, 488, 582, 697, 756, 1677, 2216
HphI	GGTGA	3 cut(s)	348, 625, 2276
Hpy188I	TCNGA	7 cut(s)	112, 196, 244, 340, 1185, 1233, 1976
Hpy99I	CGWCG	3 cut(s)	74, 859, 2069
HpyCH4III	ACNGT	9 cut(s)	572, 707, 1052, 1067, 1276, 1301, 1551, 1903, 2014
HpyCH4IV	ACGT	7 cut(s)	333, 561, 854, 894, 1228, 1490, 2064
HpyF10VI	GCNNNNNNNGC	7 cut(s)	288, 374, 377, 421, 445, 932, 2318
HpyF3I	CTNAG	5 cut(s)	19, 420, 1834, 1868, 1989
HpySE526I	ACGT	7 cut(s)	333, 561, 854, 894, 1228, 1490, 2064
Hsp92I	GRCGYC	2 cut(s)	501, 2064
HspAI	GCGC	3 cut(s)	501, 950, 1434
KasI	GGCGCC	1 cut(s)	500
Kpn2I	TCCGGA	1 cut(s)	2215
Ksp22I	TGATCA	4 cut(s)	849, 1167, 1345, 1713
LmnI	GCTCC	6 cut(s)	228, 262, 762, 808, 1583, 2005
Lsp1109I	GCAGC	2 cut(s)	922, 2296
LweI	GCATC	7 cut(s)	291, 640, 1488, 1565, 1576, 1633, 1809
MaeI	CTAG	5 cut(s)	164, 444, 515, 879, 1080
MaeII	ACGT	7 cut(s)	333, 561, 854, 894, 1228, 1490, 2064
MaeIII	GTNAC	6 cut(s)	166, 709, 1042, 1276, 1381, 1483
MbiI	CCGCTC	2 cut(s)	257, 478
MfeI	CAATTG	1 cut(s)	607
MflI	RGATCY	2 cut(s)	1968, 2235
MhlI	GDGCHC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
MluCI	AATT	7 cut(s)	95, 607, 800, 845, 913, 2158, 2180
Mly113I	GGCGCC	1 cut(s)	501
MlyI	GAGTC	4 cut(s)	152, 239, 356, 1872
MmeI	TCCRAC	3 cut(s)	205, 267, 1922
Mph1103I	ATGCAT	1 cut(s)	1933
MroI	TCCGGA	1 cut(s)	2215
MroXI	GAANNNNTTC	1 cut(s)	1970
MslI	CAYNNNNRTG	3 cut(s)	153, 1541, 1828
MspA1I	CMGCKG	3 cut(s)	506, 1781, 1838
MspCI	CTTAAG	1 cut(s)	2147
MspI	CCGG	7 cut(s)	359, 488, 582, 697, 756, 1677, 2216
MspR9I	CCNGG	8 cut(s)	401, 582, 698, 756, 757, 1678, 1688, 1766
MunI	CAATTG	1 cut(s)	607
MvaI	CCWGG	3 cut(s)	401, 1688, 1766
MwoI	GCNNNNNNNGC	7 cut(s)	288, 374, 377, 421, 445, 932, 2318
NarI	GGCGCC	1 cut(s)	501
NciI	CCSGG	5 cut(s)	582, 698, 756, 757, 1678
NcoI	CCATGG	1 cut(s)	2229
NlaIV	GGNNCC	2 cut(s)	502, 579
NmeAIII	GCCGAG	1 cut(s)	344
NmuCI	GTSAC	1 cut(s)	1483
NsiI	ATGCAT	1 cut(s)	1933
NspI	RCATGY	2 cut(s)	460, 2211
PaeR7I	CTCGAG	2 cut(s)	410, 1742
PceI	AGGCCT	1 cut(s)	1097
PciI	ACATGT	1 cut(s)	456
PdmI	GAANNNNTTC	1 cut(s)	1970
PfeI	GAWTC	4 cut(s)	107, 191, 1924, 2036
PflMI	CCANNNNNTGG	1 cut(s)	1771
PfoI	TCCNGGA	1 cut(s)	1764
Ple19I	CGATCG	1 cut(s)	524
PleI	GAGTC	4 cut(s)	152, 239, 356, 1871
PluTI	GGCGCC	1 cut(s)	504
PpsI	GAGTC	4 cut(s)	152, 239, 356, 1871
PscI	ACATGT	1 cut(s)	456
PshBI	ATTAAT	2 cut(s)	912, 2183
Psp124BI	GAGCTC	2 cut(s)	417, 1341
Psp6I	CCWGG	3 cut(s)	399, 1686, 1764
PspFI	CCCAGC	3 cut(s)	134, 526, 1838
PspGI	CCWGG	3 cut(s)	399, 1686, 1764
PspN4I	GGNNCC	2 cut(s)	502, 579
PspPI	GGNCC	4 cut(s)	217, 577, 753, 1888
PsuI	RGATCY	2 cut(s)	1968, 2235
PvuI	CGATCG	1 cut(s)	524
PvuII	CAGCTG	2 cut(s)	1781, 1838
RsaI	GTAC	1 cut(s)	1493
RsaNI	GTAC	1 cut(s)	1492
RseI	CAYNNNNRTG	3 cut(s)	153, 1541, 1828
SacI	GAGCTC	2 cut(s)	417, 1341
Sau96I	GGNCC	4 cut(s)	217, 577, 753, 1888
SchI	GAGTC	4 cut(s)	152, 239, 356, 1872
ScrFI	CCNGG	8 cut(s)	401, 582, 698, 756, 757, 1678, 1688, 1766
SduI	GDGCHC	6 cut(s)	417, 666, 1267, 1341, 1835, 2002
SfaNI	GCATC	7 cut(s)	291, 640, 1488, 1565, 1576, 1633, 1809
SfcI	CTRYAG	1 cut(s)	927
SfoI	GGCGCC	1 cut(s)	502
Sfr274I	CTCGAG	2 cut(s)	410, 1742
SinI	GGWCC	2 cut(s)	217, 577
SlaI	CTCGAG	2 cut(s)	410, 1742
SmaI	CCCGGG	1 cut(s)	757
SmiI	ATTTAAAT	1 cut(s)	2179
SmiMI	CAYNNNNRTG	3 cut(s)	153, 1541, 1828
SmlI	CTYRAG	4 cut(s)	317, 410, 1742, 2147
SmoI	CTYRAG	4 cut(s)	317, 410, 1742, 2147
SpeI	ACTAGT	1 cut(s)	878
Sse9I	AATT	7 cut(s)	95, 607, 800, 845, 913, 2158, 2180
SseBI	AGGCCT	1 cut(s)	1097
SspDI	GGCGCC	1 cut(s)	500
SspI	AATATT	1 cut(s)	1531
SspMI	CTAG	5 cut(s)	164, 444, 515, 879, 1080
SstI	GAGCTC	2 cut(s)	417, 1341
StuI	AGGCCT	1 cut(s)	1097
StyD4I	CCNGG	8 cut(s)	399, 580, 696, 754, 755, 1676, 1686, 1764
StyI	CCWWGG	2 cut(s)	1098, 2229
SwaI	ATTTAAAT	1 cut(s)	2179
TaaI	ACNGT	9 cut(s)	572, 707, 1052, 1067, 1276, 1301, 1551, 1903, 2014
TaiI	ACGT	7 cut(s)	336, 564, 857, 897, 1231, 1493, 2067
TaqI	TCGA	9 cut(s)	189, 327, 411, 453, 520, 645, 666, 1323, 1743
TaqII	GACCGA	1 cut(s)	2258
TasI	AATT	7 cut(s)	95, 607, 800, 845, 913, 2158, 2180
TauI	GCSGC	8 cut(s)	254, 257, 294, 371, 374, 377, 380, 506
TfiI	GAWTC	4 cut(s)	107, 191, 1924, 2036
TscAI	CASTG	5 cut(s)	660, 712, 775, 1443, 2323
TseFI	GTSAC	1 cut(s)	1483
TseI	GCWGC	2 cut(s)	935, 2309
Tsp45I	GTSAC	1 cut(s)	1483
TspGWI	ACGGA	1 cut(s)	58
TspMI	CCCGGG	1 cut(s)	755
TspRI	CASTG	5 cut(s)	660, 712, 775, 1443, 2323
Van91I	CCANNNNNTGG	1 cut(s)	1771
Vha464I	CTTAAG	1 cut(s)	2147
VneI	GTGCAC	1 cut(s)	1263
VpaK11BI	GGWCC	2 cut(s)	217, 577
VspI	ATTAAT	2 cut(s)	912, 2183
XapI	RAATTY	1 cut(s)	800
XceI	RCATGY	2 cut(s)	460, 2211
XhoI	CTCGAG	2 cut(s)	410, 1742
XmaI	CCCGGG	1 cut(s)	755
XmiI	GTMKAC	2 cut(s)	1366, 2019
XmnI	GAANNNNTTC	1 cut(s)	1970
XspI	CTAG	5 cut(s)	164, 444, 515, 879, 1080
ZraI	GACGTC	1 cut(s)	2065
Zsp2I	ATGCAT	1 cut(s)	1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.