Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000874

Physical Location & Seq

Genomic Coordinates Forward (+)

49019528 .. 49021864

Gene Structure 2337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G033300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGAAGAGTCTTTCACTCTCAGCCTCAAGCCACTCAAAATCCCTCCTCCACCTTCTTAAACCCCAAACCCCCCAATCCAAATCCCCCGTCTTCCTCCTCCTGAGCCGCCACCTCTGCGCAAATCCCAATTCCAATCCCAACCAACCACCCCAAGACGACTATTCCCTTGTAACCCAAGTCCTCCAACTCCTCCGACCCAATGAGAAAGACTGGAACTTTGACCAACTCCACACCCTCCTCTTCCCCAACTCAACCTCTCCCTCTCCTCGCTCCTTTTTCCTCATCACTCGCCGCCTCGGCGCTCCCTCCAAAGCCCTCAAATTCTTCGATTACGTCTCCGAAAACGTGGCCGGAACCCCTCCAGGATCCAAGGCTCTGCTCTCCACCGCATTCCAGGCCCTTCTGGAGCTCACATTGCGAGAGCCCACCTCGAAAAACAAGCTCTTTGAGCTTTACAAGATGGCCAAGGACCGGAATGTCCCGCTTAATCTCAAGGCCGCAGGTCTGCTTGTTCGGTCCATGGGCGCGGCTGGGATGGAGGACGAGGCTCTCATAGTGTTTAACGAGCTTCATTTGGGGTTGAAAACTACCCACATTCGCAATGTGATGATTGGGATGTTGCTGAAGATTGGGCGAGTGGATGATGCACTGAAGGTGCTCGACGAAATGCTTCACCCAGAAGCGAAGTTTCGGGTTGATGATTTTACCGGCGATGTTGTGATTGGCTCATTATTGAGGAGAGAGCAGATAGGGAGGAGTGTCAGTGAGGAAGAAATTGTGGAGTTGGTGTCGAGATTTGGTAAGCATGGTGTGTTTCCTAACAGCACAATACTTACGAAATTGGTCACTGTTTTGTGTAGGAGTAGGAAGGTTGGTATTGCTTGGGATGTTTTACATGATGTCATGAAGATGGGTGGTGCTGTAGAAGCTGCTCCGTGCAATGCGCTTTTGACAGCTTTGGGAAGAGGTAATGATTTTAAGAGGATGAGTGAGCTTATGGCGAAGATGGAAGAGATGGGCATTAAGCCCGATGTTATAACTTTTGGTATTCTTATCAATCGGTTATGCAAGTCTAGGAGAATAGATGCTGCCTTGGAGGTGTTTGAAAAGATGAGTGGAGGAGTAAATGGGGTTTCGGCTGAACCAGATGTGATCATCTATAACACTCTGATTGATGGACTTTGCAAATTGGGAAGGCAGGAAGAAGGATTGCGTTTGATGGAAAGGATGAGATCGCAAGGTGGCTGTGCTCCTAATACTGTTACCTACAATTGTTTGATTGATGGCTTCAACAAAGTTGGGGACATTGAGAGGGGCCGTGAGCTCTTTGATAAAATGAAGGAGGAAGGGATACCACTAAATGTGGCCACCCTTAATACTTTGCTTGATGGTCTGTGCAGGCATGGGAGACTCAACACTGCATTTGACTTCTTCAATGAAATGCAGAGGGATGGTCTGAAAGGCAATGCTGTCACTTACACAATCTTAATCACTTCCTTTTGTAATGTGAACAATATTAGCAAGGCAATGGAGTTGTTTAATCAGATGTTGAGCGCTGAATGTCCCACAGATGCAATAGTTTACCACTGCTTGATCTCTGGTTTAAGCCAAGCTGGAAGGATGGAGGATGCCAGCTTTGTTGTTTCGAAGTTGAAGGAGGCTGGTTTCTCTATGGATATTGTTTCCTACAATGTTATGATTAATGGGTTCTCCAGTAAAAATAAGCCTGATAAGATTCATGAGATGATTGAGGAAATGGAGGCGTCTGGAGTGAAGCCTGATAGCGTAACATACAACACCTTGCTTGCCTACTTAAGCAAAGCTGGGGACTTCAAAAGTGCACATAAAGTACTTGACAGGATGATGGATGAGGGTATTGTTCCCACTGTTGTCACTTTTGGTATATTGATTCATGCACATTGCTTGGATGGTGATATCGACAAAGCCATGAGAATCTTCAGAGACATGGGTTCTAAGTCAAAGATACCTCCGAATACTGTAATATACAGTGATTTAATAAATTCTCTCTGCAAGAAGAATGATGTGGAACAAGCTCTTTATTTGATGGAGGATATGAAGGAGAAAGGGGTCAGACCTAATACCCAAACGTTCAATGCCCTGTTCAAAGGCCTTAGAGAGAACAATTTATTAAAGAAGGCATTTCAATTTATGGATCGAATGGTTGAACAGGCCTGTAATCCTGATTATATAACGATGGAGATTCTTACTGAATGGCTTTCTGGTGTTGGTGAAATAGAGAGGTTGAGAAAGTTTGTTCAAGGATATCCGGTTTCAAAGCCTCAGCCCAAACAGAAGGATGGGATAGTCGCATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

778

Amino Acids

86.67

Weight (kDa)

8.06

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	297 - 353	2.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	313 - 357	1.8e-09	PPR repeat family
PPR_1	PF12854	339 - 372	2.6e-11	PPR repeat
PPR_3	PF13812	381 - 428	1.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	381 - 411	1.9e-09	PPR repeat
PPR_2	PF13041	382 - 431	6e-15	PPR repeat family
PPR_1	PF12854	415 - 447	1e-09	PPR repeat
PPR_2	PF13041	418 - 467	7.1e-16	PPR repeat family
TPR_24	PF23276	419 - 515	7.3e-07	Fungal tetratrico peptide repeats
PPR	PF01535	421 - 451	5.6e-08	PPR repeat
PPR_long	PF17177	435 - 553	4.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	450 - 482	2.3e-11	PPR repeat
PPR_2	PF13041	454 - 502	8.7e-18	PPR repeat family
PPR	PF01535	457 - 486	2.3e-07	PPR repeat
TPR_24	PF23276	467 - 572	1.7e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	485 - 516	1.5e-09	PPR repeat
PPR_2	PF13041	489 - 536	5.3e-11	PPR repeat family
PPR	PF01535	491 - 520	5e-06	PPR repeat
PPR_2	PF13041	524 - 570	1.7e-08	PPR repeat family
TPR_24	PF23276	538 - 655	1e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	559 - 602	1.1e-13	PPR repeat family
PPR	PF01535	561 - 591	4.8e-06	PPR repeat
PPR_long	PF17177	563 - 658	1.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	583 - 639	1.4e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	590 - 621	1.8e-07	PPR repeat
PPR_2	PF13041	593 - 641	7.6e-15	PPR repeat family
PPR_1	PF12854	625 - 656	1.4e-10	PPR repeat
PPR_2	PF13041	628 - 666	2.4e-06	PPR repeat family
PPR_long	PF17177	645 - 747	1.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	651 - 709	5.3e-08	Pentatricopeptide repeat domain
TPR_24	PF23276	660 - 734	5.2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	662 - 693	1.5e-07	PPR repeat
PPR_2	PF13041	664 - 711	1.1e-15	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000874)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61520 AT5G28370 AT5G28460
fragaria_vesca	FvH4_5g03011 FvH4_5g03011 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_7g15360
malus_domestica	MD01G1071400.v1.1 MD07G1131300.v1.1
prunus_persica	Prupe.2G177500_v2.0.a1
pyrus_communis	pycom01g10040 pycom04g02360 pycom07g12830
rosa_chinensis	RchiOBHm_Chr1g0357681 RchiOBHm_Chr3g0468501 RchiOBHm_Chr5g0017031 RchiOBHm_Chr7g0228211 RchiOBHm_Chr7g0228281 RchiOBHm_Chr7g0228291
rosa_laevigata	RLG00000001577 RLG00000005833 RLG00000009015 RLG00000009124 RLG00000012161 RLG00000028007 RLG00000028484
rosa_multiflora	Rmu_sc0001062.1_g000004 Rmu_sc0001626.1_g000014 Rmu_sc0001626.1_g000015 Rmu_sc0002582.1_g000009 Rmu_sc0003490.1_g000025 Rmu_sc0003490.1_g000026 Rmu_sc0004842.1_g000011 Rmu_sc0004842.1_g000012 Rmu_sc0004842.1_g000020 Rmu_ssc0000425.1_g000002
rosa_roxburghii	Rroxscaffold_3G00231300 Rroxscaffold_4G00298600 Rroxscaffold_6G00429570
rosa_rugosa	Rorug01G0257700 Rorug02G0051500 Rorug02G0572600 Rorug07G0246900.1 Rorug07G0247000 Rorug07G0247000
rosa_samantha	Rh1AG271700 Rh1BG238800 Rh1CG255600 Rh1DG267200 Rh3AG143700 Rh3BG166200 Rh3BG166300 Rh5AG043600 Rh7AG400900 Rh7AG401200 Rh7BG382400 Rh7CG420000 Rh7DG396900 Rh7DG397300
rosa_wichuraiana	Rw1G024230 Rw7G033300 Rw7G033330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1037
AasI	GACNNNNNNGTC	1 cut(s)	476
Acc16I	TGCGCA	1 cut(s)	118
Acc36I	ACCTGC	1 cut(s)	491
AccII	CGCG	1 cut(s)	527
AciI	CCGC	6 cut(s)	106, 292, 387, 482, 498, 527
AclI	AACGTT	1 cut(s)	2108
AclWI	GGATC	3 cut(s)	360, 373, 2181
AcoI	YGGCCR	3 cut(s)	348, 462, 1365
AcsI	RAATTY	2 cut(s)	320, 2020
AcuI	CTGAAG	3 cut(s)	644, 671, 1942
AcyI	GRCGYC	1 cut(s)	1763
AfaI	GTAC	1 cut(s)	1851
AfeI	AGCGCT	1 cut(s)	1555
AfiI	CCNNNNNNNGG	1 cut(s)	471
AflII	CTTAAG	1 cut(s)	1813
AjnI	CCWGG	2 cut(s)	361, 393
Alw21I	GWGCWC	5 cut(s)	411, 660, 1252, 1326, 1843
Alw26I	GTCTC	3 cut(s)	340, 1402, 1956
Alw44I	GTGCAC	1 cut(s)	1839
AlwI	GGATC	3 cut(s)	360, 373, 2181
Aor51HI	AGCGCT	1 cut(s)	1555
AoxI	GGCC	8 cut(s)	348, 396, 462, 495, 1315, 1365, 2128, 2190
ApaLI	GTGCAC	1 cut(s)	1839
ApeKI	GCWGC	2 cut(s)	929, 1088
ApoI	RAATTY	2 cut(s)	320, 2020
ArsI	GACNNNNNNTTYG	4 cut(s)	72, 104, 200, 232
AseI	ATTAAT	1 cut(s)	1701
Asp700I	GAANNNNTTC	1 cut(s)	669
AspLEI	GCGC	5 cut(s)	119, 302, 527, 946, 1556
AspS9I	GGNCC	4 cut(s)	397, 469, 516, 1315
AsuHPI	GGTGA	3 cut(s)	665, 1943, 2261
AsuII	TTCGAA	1 cut(s)	1646
AvaII	GGWCC	2 cut(s)	469, 516
BaeGI	GKGCMC	1 cut(s)	1843
BalI	TGGCCA	2 cut(s)	464, 1367
BamHI	GGATCC	1 cut(s)	365
BanII	GRGCYC	3 cut(s)	411, 426, 1326
BbsI	GAAGAC	1 cut(s)	82
Bbv12I	GWGCWC	5 cut(s)	411, 660, 1252, 1326, 1843
BbvCI	CCTCAGC	1 cut(s)	2301
BbvI	GCAGC	2 cut(s)	916, 1075
BceAI	ACGGC	1 cut(s)	1302
BciT130I	CCWGG	2 cut(s)	363, 395
BciVI	GTATCC	1 cut(s)	1344
BclI	TGATCA	1 cut(s)	1152
BcoDI	GTCTC	3 cut(s)	340, 1402, 1956
BfaI	CTAG	1 cut(s)	1074
BfmI	CTRYAG	1 cut(s)	921
BfoI	RGCGCY	2 cut(s)	303, 1557
BfrI	CTTAAG	1 cut(s)	1813
BfuAI	ACCTGC	1 cut(s)	491
BfuI	GTATCC	1 cut(s)	1344
BglI	GCCNNNNNGGC	1 cut(s)	297
BisI	GCNGC	6 cut(s)	106, 292, 498, 528, 930, 1089
BlsI	GCNGC	6 cut(s)	107, 293, 499, 529, 931, 1090
BmcAI	AGTACT	1 cut(s)	1851
Bme1390I	CCNGG	2 cut(s)	363, 395
Bme18I	GGWCC	2 cut(s)	469, 516
BmgT120I	GGNCC	4 cut(s)	397, 469, 516, 1315
BmiI	GGNNCC	3 cut(s)	355, 367, 1316
BmrFI	CCNGG	2 cut(s)	363, 395
BmsI	GCATC	4 cut(s)	634, 1075, 1561, 1618
BpiI	GAAGAC	1 cut(s)	82
BplI	GAGNNNNNCTC	2 cut(s)	413, 445
BpmI	CTGGAG	4 cut(s)	345, 425, 1696, 1788
Bpu10I	CCTNAGC	2 cut(s)	101, 2301
Bpu14I	TTCGAA	1 cut(s)	1646
BpuEI	CTTGAG	2 cut(s)	10, 476
BsaHI	GRCGYC	1 cut(s)	1763
BsaJI	CCNNGG	5 cut(s)	295, 369, 465, 519, 1092
BsaWI	WCCGGW	2 cut(s)	471, 2287
BsaXI	ACNNNNNCTCC	4 cut(s)	748, 773, 778, 803
Bsc4I	CCNNNNNNNGG	1 cut(s)	471
Bse118I	RCCGGY	1 cut(s)	707
Bse1I	ACTGG	2 cut(s)	215, 1713
Bse3DI	GCAATG	6 cut(s)	413, 607, 946, 1471, 1533, 1918
BseBI	CCWGG	2 cut(s)	363, 395
BseDI	CCNNGG	5 cut(s)	295, 369, 465, 519, 1092
BseLI	CCNNNNNNNGG	1 cut(s)	471
BseMI	GCAATG	6 cut(s)	413, 607, 946, 1471, 1533, 1918
BseMII	CTCAG	3 cut(s)	33, 92, 2315
BseNI	ACTGG	2 cut(s)	215, 1713
BseRI	GAGGAG	8 cut(s)	35, 86, 179, 227, 255, 751, 769, 1134
BseSI	GKGCMC	1 cut(s)	1843
BseXI	GCAGC	2 cut(s)	916, 1075
BseYI	CCCAGC	2 cut(s)	530, 1823
BsgI	GTGCAG	1 cut(s)	1417
Bsh1236I	CGCG	1 cut(s)	527
BshFI	GGCC	8 cut(s)	350, 398, 464, 497, 1317, 1367, 2130, 2192
BsiHKAI	GWGCWC	5 cut(s)	411, 660, 1252, 1326, 1843
BsiSI	CCGG	4 cut(s)	351, 472, 708, 2288
BslFI	GGGAC	4 cut(s)	464, 1316, 1548, 1841
BslI	CCNNNNNNNGG	1 cut(s)	471
BsmAI	GTCTC	3 cut(s)	340, 1402, 1956
BsmBI	CGTCTC	1 cut(s)	340
BsmFI	GGGAC	4 cut(s)	464, 1316, 1548, 1841
BsmI	GAATGC	1 cut(s)	389
BsnI	GGCC	8 cut(s)	350, 398, 464, 497, 1317, 1367, 2130, 2192
Bsp119I	TTCGAA	1 cut(s)	1646
Bsp1286I	GDGCHC	6 cut(s)	411, 426, 660, 1252, 1326, 1843
Bsp143I	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
Bsp19I	CCATGG	1 cut(s)	519
BspACI	CCGC	6 cut(s)	106, 292, 387, 482, 498, 527
BspANI	GGCC	8 cut(s)	350, 398, 464, 497, 1317, 1367, 2130, 2192
BspCNI	CTCAG	3 cut(s)	32, 93, 2314
BspFNI	CGCG	1 cut(s)	527
BspHI	TCATGA	2 cut(s)	903, 1738
BspLI	GGNNCC	3 cut(s)	355, 367, 1316
BspMI	ACCTGC	1 cut(s)	491
BspPI	GGATC	3 cut(s)	360, 373, 2181
BspT104I	TTCGAA	1 cut(s)	1646
BspTI	CTTAAG	1 cut(s)	1813
BsrDI	GCAATG	6 cut(s)	413, 607, 946, 1471, 1533, 1918
BsrFI	RCCGGY	1 cut(s)	707
BsrI	ACTGG	2 cut(s)	215, 1713
BssAI	RCCGGY	1 cut(s)	707
BssECI	CCNNGG	5 cut(s)	295, 369, 465, 519, 1092
BssMI	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
BssNI	GRCGYC	1 cut(s)	1763
BssT1I	CCWWGG	4 cut(s)	369, 465, 519, 1092
Bst2UI	CCWGG	2 cut(s)	363, 395
Bst4CI	ACNGT	5 cut(s)	850, 1261, 1888, 1999, 2009
Bst6I	CTCTTC	3 cut(s)	245, 958, 1005
BstACI	GRCGYC	1 cut(s)	1763
BstAFI	CTTAAG	1 cut(s)	1813
BstBI	TTCGAA	1 cut(s)	1646
BstC8I	GCNNGC	3 cut(s)	1400, 1633, 1806
BstDEI	CTNAG	5 cut(s)	19, 101, 1974, 2132, 2301
BstDSI	CCRYGG	1 cut(s)	519
BstFNI	CGCG	1 cut(s)	527
BstH2I	RGCGCY	2 cut(s)	303, 1557
BstHHI	GCGC	5 cut(s)	119, 302, 527, 946, 1556
BstKTI	GATC	5 cut(s)	368, 1155, 1235, 1596, 2176
BstMAI	GTCTC	3 cut(s)	340, 1402, 1956
BstMBI	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
BstMWI	GCNNNNNNNGC	6 cut(s)	114, 297, 395, 415, 448, 926
BstNI	CCWGG	2 cut(s)	363, 395
BstSCI	CCNGG	2 cut(s)	361, 393
BstSFI	CTRYAG	1 cut(s)	921
BstSLI	GKGCMC	1 cut(s)	1843
BstUI	CGCG	1 cut(s)	527
BstV1I	GCAGC	2 cut(s)	916, 1075
BstV2I	GAAGAC	1 cut(s)	82
BstX2I	RGATCY	1 cut(s)	365
BstYI	RGATCY	1 cut(s)	365
BsuI	GTATCC	1 cut(s)	1344
BsuRI	GGCC	8 cut(s)	350, 398, 464, 497, 1317, 1367, 2130, 2192
BtgI	CCRYGG	1 cut(s)	519
BtgZI	GCGATG	1 cut(s)	726
BtsI	GCAGTG	2 cut(s)	1416, 1585
BtsIMutI	CAGTG	7 cut(s)	647, 769, 846, 1416, 1585, 1884, 2014
BveI	ACCTGC	1 cut(s)	491
Cac8I	GCNNGC	3 cut(s)	1400, 1633, 1806
CciI	TCATGA	2 cut(s)	903, 1738
CfoI	GCGC	5 cut(s)	119, 302, 527, 946, 1556
Cfr10I	RCCGGY	1 cut(s)	707
Cfr13I	GGNCC	4 cut(s)	397, 469, 516, 1315
CseI	GACGC	1 cut(s)	1752
Csp6I	GTAC	1 cut(s)	1850
CviAII	CATG	9 cut(s)	520, 806, 896, 904, 1403, 1739, 1913, 1948, 1966
CviQI	GTAC	1 cut(s)	1850
DdeI	CTNAG	5 cut(s)	19, 101, 1974, 2132, 2301
DpnI	GATC	5 cut(s)	367, 1154, 1234, 1595, 2175
DpnII	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
DrdI	GACNNNNNNGTC	1 cut(s)	476
DseDI	GACNNNNNNGTC	1 cut(s)	476
EaeI	YGGCCR	3 cut(s)	348, 462, 1365
Eam1104I	CTCTTC	3 cut(s)	245, 958, 1005
EarI	CTCTTC	3 cut(s)	245, 958, 1005
Ecl136II	GAGCTC	2 cut(s)	409, 1324
Eco130I	CCWWGG	4 cut(s)	369, 465, 519, 1092
Eco147I	AGGCCT	2 cut(s)	2130, 2192
Eco24I	GRGCYC	3 cut(s)	411, 426, 1326
Eco32I	GATATC	2 cut(s)	1936, 2285
Eco47I	GGWCC	2 cut(s)	469, 516
Eco47III	AGCGCT	1 cut(s)	1555
Eco53kI	GAGCTC	2 cut(s)	409, 1324
Eco57I	CTGAAG	3 cut(s)	644, 671, 1942
EcoICRI	GAGCTC	2 cut(s)	409, 1324
EcoO109I	RGGNCCY	1 cut(s)	397
EcoRII	CCWGG	2 cut(s)	361, 393
EcoRV	GATATC	2 cut(s)	1936, 2285
EcoT14I	CCWWGG	4 cut(s)	369, 465, 519, 1092
EcoT38I	GRGCYC	3 cut(s)	411, 426, 1326
ErhI	CCWWGG	4 cut(s)	369, 465, 519, 1092
Esp3I	CGTCTC	1 cut(s)	340
FaeI	CATG	9 cut(s)	523, 809, 899, 907, 1406, 1742, 1916, 1951, 1969
FaqI	GGGAC	4 cut(s)	464, 1316, 1548, 1841
FatI	CATG	9 cut(s)	519, 805, 895, 903, 1402, 1738, 1912, 1947, 1965
FauI	CCCGC	1 cut(s)	489
FbaI	TGATCA	1 cut(s)	1152
Fnu4HI	GCNGC	6 cut(s)	106, 292, 498, 528, 930, 1089
FriOI	GRGCYC	3 cut(s)	411, 426, 1326
Fsp4HI	GCNGC	6 cut(s)	106, 292, 498, 528, 930, 1089
FspBI	CTAG	1 cut(s)	1074
FspI	TGCGCA	1 cut(s)	118
GlaI	GCGC	5 cut(s)	118, 301, 526, 945, 1555
GluI	GCNGC	6 cut(s)	106, 292, 498, 528, 930, 1089
GsaI	CCCAGC	2 cut(s)	534, 1827
GsuI	CTGGAG	4 cut(s)	345, 425, 1696, 1788
HaeII	RGCGCY	2 cut(s)	303, 1557
HaeIII	GGCC	8 cut(s)	350, 398, 464, 497, 1317, 1367, 2130, 2192
HapII	CCGG	4 cut(s)	351, 472, 708, 2288
HgaI	GACGC	1 cut(s)	1752
HhaI	GCGC	5 cut(s)	119, 302, 527, 946, 1556
Hin1I	GRCGYC	1 cut(s)	1763
Hin1II	CATG	9 cut(s)	523, 809, 899, 907, 1406, 1742, 1916, 1951, 1969
Hin6I	GCGC	5 cut(s)	117, 300, 525, 944, 1554
HinP1I	GCGC	5 cut(s)	117, 300, 525, 944, 1554
HinfI	GANTC	6 cut(s)	7, 1410, 1735, 1909, 1953, 2221
HpaII	CCGG	4 cut(s)	351, 472, 708, 2288
HphI	GGTGA	3 cut(s)	665, 1943, 2261
Hpy166II	GTNNAC	3 cut(s)	1510, 1582, 1841
Hpy188I	TCNGA	8 cut(s)	194, 340, 1170, 1458, 1545, 1961, 1992, 2093
Hpy188III	TCNNGA	7 cut(s)	100, 404, 792, 904, 1739, 1767, 2201
Hpy8I	GTNNAC	3 cut(s)	1510, 1582, 1841
Hpy99I	CGWCG	1 cut(s)	665
HpyCH4III	ACNGT	5 cut(s)	850, 1261, 1888, 1999, 2009
HpyCH4IV	ACGT	3 cut(s)	333, 345, 2108
HpyF10VI	GCNNNNNNNGC	6 cut(s)	114, 297, 395, 415, 448, 926
HpyF3I	CTNAG	5 cut(s)	19, 101, 1974, 2132, 2301
HpySE526I	ACGT	3 cut(s)	333, 345, 2108
Hsp92I	GRCGYC	1 cut(s)	1763
Hsp92II	CATG	9 cut(s)	523, 809, 899, 907, 1406, 1742, 1916, 1951, 1969
HspAI	GCGC	5 cut(s)	117, 300, 525, 944, 1554
Ksp22I	TGATCA	1 cut(s)	1152
Kzo9I	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
LmnI	GCTCC	5 cut(s)	275, 307, 406, 937, 1255
Lsp1109I	GCAGC	2 cut(s)	916, 1075
LweI	GCATC	4 cut(s)	634, 1075, 1561, 1618
MaeI	CTAG	1 cut(s)	1074
MaeII	ACGT	3 cut(s)	333, 345, 2108
MaeIII	GTNAC	6 cut(s)	169, 844, 1261, 1471, 1786, 1891
MalI	GATC	5 cut(s)	367, 1154, 1234, 1595, 2175
MboI	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
MfeI	CAATTG	1 cut(s)	1270
MflI	RGATCY	1 cut(s)	365
MhlI	GDGCHC	6 cut(s)	411, 426, 660, 1252, 1326, 1843
MlsI	TGGCCA	2 cut(s)	464, 1367
MluCI	AATT	9 cut(s)	127, 320, 774, 839, 1187, 1270, 2020, 2143, 2165
MluNI	TGGCCA	2 cut(s)	464, 1367
MlyI	GAGTC	2 cut(s)	16, 1404
MmeI	TCCRAC	2 cut(s)	208, 217
Mox20I	TGGCCA	2 cut(s)	464, 1367
MroXI	GAANNNNTTC	1 cut(s)	669
MscI	TGGCCA	2 cut(s)	464, 1367
MslI	CAYNNNNRTG	1 cut(s)	908
Msp20I	TGGCCA	2 cut(s)	464, 1367
MspCI	CTTAAG	1 cut(s)	1813
MspI	CCGG	4 cut(s)	351, 472, 708, 2288
MspR9I	CCNGG	2 cut(s)	363, 395
MunI	CAATTG	1 cut(s)	1270
Mva1269I	GAATGC	1 cut(s)	389
MvaI	CCWGG	2 cut(s)	363, 395
MvnI	CGCG	1 cut(s)	527
MwoI	GCNNNNNNNGC	6 cut(s)	114, 297, 395, 415, 448, 926
NcoI	CCATGG	1 cut(s)	519
NdeII	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
NlaIII	CATG	9 cut(s)	523, 809, 899, 907, 1406, 1742, 1916, 1951, 1969
NlaIV	GGNNCC	3 cut(s)	355, 367, 1316
NmeAIII	GCCGAG	1 cut(s)	276
NmuCI	GTSAC	3 cut(s)	844, 1471, 1891
NsbI	TGCGCA	1 cut(s)	118
NspV	TTCGAA	1 cut(s)	1646
PagI	TCATGA	2 cut(s)	903, 1738
PceI	AGGCCT	2 cut(s)	2130, 2192
PctI	GAATGC	1 cut(s)	389
PdmI	GAANNNNTTC	1 cut(s)	669
PfeI	GAWTC	4 cut(s)	1735, 1909, 1953, 2221
PfoI	TCCNGGA	1 cut(s)	361
PkrI	GCNGC	6 cut(s)	107, 293, 499, 529, 931, 1090
PleI	GAGTC	2 cut(s)	15, 1404
PpsI	GAGTC	2 cut(s)	15, 1404
PshBI	ATTAAT	1 cut(s)	1701
PsiI	TTATAA	1 cut(s)	1037
Psp124BI	GAGCTC	2 cut(s)	411, 1326
Psp1406I	AACGTT	1 cut(s)	2108
Psp6I	CCWGG	2 cut(s)	361, 393
PspFI	CCCAGC	2 cut(s)	530, 1823
PspGI	CCWGG	2 cut(s)	361, 393
PspN4I	GGNNCC	3 cut(s)	355, 367, 1316
PspPI	GGNCC	4 cut(s)	397, 469, 516, 1315
PsuI	RGATCY	1 cut(s)	365
RsaI	GTAC	1 cut(s)	1851
RsaNI	GTAC	1 cut(s)	1850
RseI	CAYNNNNRTG	1 cut(s)	908
SacI	GAGCTC	2 cut(s)	411, 1326
SatI	GCNGC	6 cut(s)	106, 292, 498, 528, 930, 1089
Sau3AI	GATC	5 cut(s)	365, 1152, 1232, 1593, 2173
Sau96I	GGNCC	4 cut(s)	397, 469, 516, 1315
ScaI	AGTACT	1 cut(s)	1851
SchI	GAGTC	2 cut(s)	16, 1404
ScrFI	CCNGG	2 cut(s)	363, 395
SduI	GDGCHC	6 cut(s)	411, 426, 660, 1252, 1326, 1843
SfaNI	GCATC	4 cut(s)	634, 1075, 1561, 1618
SfcI	CTRYAG	1 cut(s)	921
SfuI	TTCGAA	1 cut(s)	1646
SinI	GGWCC	2 cut(s)	469, 516
SmiMI	CAYNNNNRTG	1 cut(s)	908
SmlI	CTYRAG	3 cut(s)	25, 491, 1813
SmoI	CTYRAG	3 cut(s)	25, 491, 1813
Sse9I	AATT	9 cut(s)	127, 320, 774, 839, 1187, 1270, 2020, 2143, 2165
SseBI	AGGCCT	2 cut(s)	2130, 2192
SsiI	CCGC	6 cut(s)	106, 292, 387, 482, 498, 527
SspI	AATATT	1 cut(s)	1516
SspMI	CTAG	1 cut(s)	1074
SstI	GAGCTC	2 cut(s)	411, 1326
StuI	AGGCCT	2 cut(s)	2130, 2192
StyD4I	CCNGG	2 cut(s)	361, 393
StyI	CCWWGG	4 cut(s)	369, 465, 519, 1092
TaaI	ACNGT	5 cut(s)	850, 1261, 1888, 1999, 2009
TaiI	ACGT	3 cut(s)	336, 348, 2111
TaqI	TCGA	7 cut(s)	327, 431, 660, 791, 1646, 1938, 2176
TaqII	GACCGA	1 cut(s)	504
TasI	AATT	9 cut(s)	127, 320, 774, 839, 1187, 1270, 2020, 2143, 2165
TatI	WGTACW	1 cut(s)	1849
TauI	GCSGC	4 cut(s)	108, 294, 500, 530
TfiI	GAWTC	4 cut(s)	1735, 1909, 1953, 2221
TscAI	CASTG	7 cut(s)	654, 769, 853, 1423, 1592, 1891, 2014
TseFI	GTSAC	3 cut(s)	844, 1471, 1891
TseI	GCWGC	2 cut(s)	929, 1088
Tsp45I	GTSAC	3 cut(s)	844, 1471, 1891
TspDTI	ATGAA	8 cut(s)	17, 560, 920, 1352, 1452, 1727, 1901, 2090
TspGWI	ACGGA	1 cut(s)	924
TspRI	CASTG	7 cut(s)	654, 769, 853, 1423, 1592, 1891, 2014
Vha464I	CTTAAG	1 cut(s)	1813
VneI	GTGCAC	1 cut(s)	1839
VpaK11BI	GGWCC	2 cut(s)	469, 516
VspI	ATTAAT	1 cut(s)	1701
XapI	RAATTY	2 cut(s)	320, 2020
XmnI	GAANNNNTTC	1 cut(s)	669
XspI	CTAG	1 cut(s)	1074
ZrmI	AGTACT	1 cut(s)	1851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.