Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000874

Physical Location & Seq

Genomic Coordinates Forward (+)

51621695 .. 51623220

Gene Structure 1526 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ20449

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGTGAATAAGAAGTTTGGTATTGCTTGGGATGTTTTGCATGATGTCATGAAGATGGGTGGTGCTGTGGAAGCTGCTCCGTGCAATGCGCTTTTGACAGCTTTGGGGAGAGGTAATGATTTTAAGAGGATGAGTGAGCTTATGGCGAAGATGGAAGAGATGGGCATTAAGCCCGATGTTATAACTTTTGGTATTCTTATTAATCGGTTATGCAAGTCTAGGAGAATAGATGCTGCCTTGGAGGTGTTTGAAACGATGAGTGGAGGGGTAAAGGGGGTTTCCGCTGAACCAGATGTGATCGTCTATAACACTCTGATTGATGGACTTTGCAAAGTGGGAAGGCAGGAAGAAGGATTGCGTTTGATGGAAAGGATGAGATCGCAAGGCGGCTGTGCTCCTAATACTGTTACCTACAATTGTTTGATTGATGGTTTCAACAAAGTTGGGGACATTGAGAGGGGCCGTGAGCTCTTTGATAAAATGAAGGAAGAAGGGATACCACTAAATGTGGAGACCCTTAATACTTTGCTTGATGGTCTGTGCAGGCATCGGAGACTCAACACTGCATTTGACTTCTTCAATGAAATGCAGAGGGATGGTCTGAAAGGCAATGCTGTCACTTACACAATCTTAATCACTTCCCTTTGTAATGTGAACAATATTAGCAAGGCAATGGAGTTGTTTAATCAGATGTTGAGCGCTGAATGTCCCACAGATGCAATAGTTTACCACTGCTTGATCTCTGGTTTAAGCCAAGCTGGAAGGATGGAGGATGCCAGCTTTGTTGTTTCGAAGTTGAAGGAGGCTGGTTTCTCTATGGATATCGTTTCCTACAATGTTATGATTAATGGGTTCTCCAGTAAAAATAAGCCTGATAAGATTCATGAGATGATTGAGGAAATGGAGGCGTCTGGAGTGAAGCCTGATAGCGTAACATACAACACGTTGCTTGCCTACTTAAGCAAAGCTGAGGACTTCAAAAGTGCACATAAAGTACTTGACAGGATGATGGATGAGGGTATTATTCCCACTGCTTTTGGTACATTGATTCATGCACATTGCTTGGATGGTGATATCGACAAAGCCATGAGAATCTTCAGAGACATGGGTTCTAAGTCAAAGATACCTCCGAATACTGTAATATACAGTGATTTAATAAATTCTCTCTGCAAGAAGAATGATGTGGAACAAGCTCTTTATTTGATGGAGGATATGAAGGAGAAAGGGGTGAGACCTAATACCCAAAGGTTCAATGCCCTGTTCAAAGCCCTTAGAGAGAACAATTTATTAAAGAAGGCACTTCAATTTATGGATCGAATGGTTGAACAGGCCTGTAATCCTGATTATATAACGATGGAGATTCTTACTGAATGGCTTTCTGGTGTTGGTGAAATAGAGAGGTTGAGAAAGTTTGTTCAAGGATATCCGGTTTCGAAGCCTCAGCCCCAACAGAAGGATGGGATAGTCGCATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

491

Amino Acids

54.96

Weight (kDa)

5.95

Isoelectric Point (pI)

31.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	12 - 68	1.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	28 - 72	1e-09	PPR repeat family
PPR_1	PF12854	54 - 86	7e-11	PPR repeat
PPR_3	PF13812	96 - 144	1.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	96 - 126	7.3e-10	PPR repeat
PPR_2	PF13041	97 - 146	2.4e-15	PPR repeat family
PPR_1	PF12854	130 - 162	5.9e-10	PPR repeat
PPR_2	PF13041	133 - 182	8.9e-16	PPR repeat family
TPR_24	PF23276	135 - 230	1.2e-07	Fungal tetratrico peptide repeats
PPR	PF01535	136 - 166	3.2e-08	PPR repeat
PPR_long	PF17177	142 - 274	3.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	165 - 197	9.1e-10	PPR repeat
PPR_2	PF13041	169 - 217	4.3e-17	PPR repeat family
PPR	PF01535	172 - 201	1.1e-06	PPR repeat
PPR_1	PF12854	200 - 231	1.4e-09	PPR repeat
PPR_2	PF13041	204 - 251	3.3e-11	PPR repeat family
PPR	PF01535	206 - 235	4.5e-06	PPR repeat
PPR_long	PF17177	208 - 344	4.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	239 - 285	9.5e-09	PPR repeat family
TPR_24	PF23276	253 - 368	1.2e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	274 - 317	4.5e-14	PPR repeat family
PPR	PF01535	276 - 306	2.7e-06	PPR repeat
PPR_3	PF13812	298 - 344	8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	305 - 336	3.6e-06	PPR repeat
PPR_2	PF13041	308 - 346	2.5e-08	PPR repeat family
PPR_1	PF12854	346 - 369	9.2e-06	PPR repeat
PPR_long	PF17177	349 - 461	3.3e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	374 - 449	2.7e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	375 - 424	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	375 - 406	8.5e-08	PPR repeat
PPR_2	PF13041	377 - 424	1.3e-12	PPR repeat family
PPR	PF01535	380 - 410	6.6e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000874)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61520 AT5G28370 AT5G28460
fragaria_vesca	FvH4_5g03011 FvH4_5g03011 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_7g15360
malus_domestica	MD01G1071400.v1.1 MD07G1131300.v1.1
prunus_persica	Prupe.2G177500_v2.0.a1
pyrus_communis	pycom01g10040 pycom04g02360 pycom07g12830
rosa_chinensis	RchiOBHm_Chr1g0357681 RchiOBHm_Chr3g0468501 RchiOBHm_Chr5g0017031 RchiOBHm_Chr7g0228211 RchiOBHm_Chr7g0228281 RchiOBHm_Chr7g0228291
rosa_laevigata	RLG00000001577 RLG00000005833 RLG00000009015 RLG00000009124 RLG00000012161 RLG00000028007 RLG00000028484
rosa_multiflora	Rmu_sc0001062.1_g000004 Rmu_sc0001626.1_g000014 Rmu_sc0001626.1_g000015 Rmu_sc0002582.1_g000009 Rmu_sc0003490.1_g000025 Rmu_sc0003490.1_g000026 Rmu_sc0004842.1_g000011 Rmu_sc0004842.1_g000012 Rmu_sc0004842.1_g000020 Rmu_ssc0000425.1_g000002
rosa_roxburghii	Rroxscaffold_3G00231300 Rroxscaffold_4G00298600 Rroxscaffold_6G00429570
rosa_rugosa	Rorug01G0257700 Rorug02G0051500 Rorug02G0572600 Rorug07G0246900.1 Rorug07G0247000 Rorug07G0247000
rosa_samantha	Rh1AG271700 Rh1BG238800 Rh1CG255600 Rh1DG267200 Rh3AG143700 Rh3BG166200 Rh3BG166300 Rh5AG043600 Rh7AG400900 Rh7AG401200 Rh7BG382400 Rh7CG420000 Rh7DG396900 Rh7DG397300
rosa_wichuraiana	Rw1G024230 Rw7G033300 Rw7G033330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	182
AciI	CCGC	2 cut(s)	282, 387
AclWI	GGATC	1 cut(s)	1320
AcsI	RAATTY	1 cut(s)	1159
AcuI	CTGAAG	1 cut(s)	1081
AcyI	GRCGYC	1 cut(s)	908
AfaI	GTAC	2 cut(s)	996, 1042
AfeI	AGCGCT	1 cut(s)	700
AfiI	CCNNNNNNNGG	1 cut(s)	1453
AflII	CTTAAG	1 cut(s)	958
AflIII	ACRYGT	1 cut(s)	942
AluBI	AGCT	8 cut(s)	74, 101, 139, 469, 758, 780, 968, 1193
AluI	AGCT	8 cut(s)	74, 101, 139, 469, 758, 780, 968, 1193
Alw21I	GWGCWC	3 cut(s)	397, 471, 988
Alw26I	GTCTC	4 cut(s)	506, 547, 1095, 1225
Alw44I	GTGCAC	1 cut(s)	984
AlwI	GGATC	1 cut(s)	1320
Aor51HI	AGCGCT	1 cut(s)	700
AoxI	GGCC	2 cut(s)	460, 1329
ApaLI	GTGCAC	1 cut(s)	984
ApeKI	GCWGC	2 cut(s)	74, 233
ApoI	RAATTY	1 cut(s)	1159
AseI	ATTAAT	2 cut(s)	201, 846
AspLEI	GCGC	2 cut(s)	91, 701
AspS9I	GGNCC	1 cut(s)	460
AsuHPI	GGTGA	4 cut(s)	16, 1082, 1240, 1400
AsuII	TTCGAA	2 cut(s)	791, 1433
BaeGI	GKGCMC	1 cut(s)	988
BanII	GRGCYC	1 cut(s)	471
BarI	GAAGNNNNNNTAC	2 cut(s)	480, 512
Bbv12I	GWGCWC	3 cut(s)	397, 471, 988
BbvCI	CCTCAGC	2 cut(s)	969, 1440
BbvI	GCAGC	2 cut(s)	61, 220
BceAI	ACGGC	1 cut(s)	447
BciVI	GTATCC	1 cut(s)	489
BcoDI	GTCTC	4 cut(s)	506, 547, 1095, 1225
BfaI	CTAG	1 cut(s)	219
BfoI	RGCGCY	1 cut(s)	702
BfrI	CTTAAG	1 cut(s)	958
BfuI	GTATCC	1 cut(s)	489
BisI	GCNGC	3 cut(s)	75, 234, 388
BlsI	GCNGC	3 cut(s)	76, 235, 389
BmcAI	AGTACT	1 cut(s)	996
BmgT120I	GGNCC	1 cut(s)	460
BmiI	GGNNCC	1 cut(s)	461
BmsI	GCATC	4 cut(s)	220, 556, 706, 763
BpmI	CTGGAG	2 cut(s)	841, 933
Bpu10I	CCTNAGC	2 cut(s)	969, 1440
Bpu14I	TTCGAA	2 cut(s)	791, 1433
BsaHI	GRCGYC	1 cut(s)	908
BsaI	GGTCTC	2 cut(s)	506, 1225
BsaJI	CCNNGG	1 cut(s)	237
BsaWI	WCCGGW	1 cut(s)	1426
Bsc4I	CCNNNNNNNGG	1 cut(s)	1453
Bse1I	ACTGG	1 cut(s)	858
Bse3DI	GCAATG	4 cut(s)	91, 616, 678, 1057
BseDI	CCNNGG	1 cut(s)	237
BseLI	CCNNNNNNNGG	1 cut(s)	1453
BseMI	GCAATG	4 cut(s)	91, 616, 678, 1057
BseMII	CTCAG	2 cut(s)	960, 1454
BseNI	ACTGG	1 cut(s)	858
BseSI	GKGCMC	1 cut(s)	988
BseXI	GCAGC	2 cut(s)	61, 220
BsgI	GTGCAG	1 cut(s)	562
BshFI	GGCC	2 cut(s)	462, 1331
BsiHKAI	GWGCWC	3 cut(s)	397, 471, 988
BsiSI	CCGG	1 cut(s)	1427
BslFI	GGGAC	2 cut(s)	461, 693
BslI	CCNNNNNNNGG	1 cut(s)	1453
BsmAI	GTCTC	4 cut(s)	506, 547, 1095, 1225
BsmFI	GGGAC	2 cut(s)	461, 693
BsnI	GGCC	2 cut(s)	462, 1331
Bso31I	GGTCTC	2 cut(s)	506, 1225
Bsp119I	TTCGAA	2 cut(s)	791, 1433
Bsp1286I	GDGCHC	3 cut(s)	397, 471, 988
Bsp143I	GATC	4 cut(s)	297, 377, 738, 1312
BspACI	CCGC	2 cut(s)	282, 387
BspANI	GGCC	2 cut(s)	462, 1331
BspCNI	CTCAG	2 cut(s)	961, 1453
BspHI	TCATGA	2 cut(s)	48, 883
BspLI	GGNNCC	1 cut(s)	461
BspPI	GGATC	1 cut(s)	1320
BspT104I	TTCGAA	2 cut(s)	791, 1433
BspTI	CTTAAG	1 cut(s)	958
BspTNI	GGTCTC	2 cut(s)	506, 1225
BsrDI	GCAATG	4 cut(s)	91, 616, 678, 1057
BsrI	ACTGG	1 cut(s)	858
BssECI	CCNNGG	1 cut(s)	237
BssMI	GATC	4 cut(s)	297, 377, 738, 1312
BssNI	GRCGYC	1 cut(s)	908
BssT1I	CCWWGG	1 cut(s)	237
Bst4CI	ACNGT	3 cut(s)	406, 1138, 1148
Bst6I	CTCTTC	1 cut(s)	150
BstACI	GRCGYC	1 cut(s)	908
BstAFI	CTTAAG	1 cut(s)	958
BstBI	TTCGAA	2 cut(s)	791, 1433
BstC8I	GCNNGC	3 cut(s)	545, 778, 951
BstDEI	CTNAG	4 cut(s)	969, 1113, 1271, 1440
BstH2I	RGCGCY	1 cut(s)	702
BstHHI	GCGC	2 cut(s)	91, 701
BstKTI	GATC	4 cut(s)	300, 380, 741, 1315
BstMAI	GTCTC	4 cut(s)	506, 547, 1095, 1225
BstMBI	GATC	4 cut(s)	297, 377, 738, 1312
BstMWI	GCNNNNNNNGC	1 cut(s)	71
BstSLI	GKGCMC	1 cut(s)	988
BstV1I	GCAGC	2 cut(s)	61, 220
BsuI	GTATCC	1 cut(s)	489
BsuRI	GGCC	2 cut(s)	462, 1331
BtsI	GCAGTG	3 cut(s)	561, 730, 1029
BtsIMutI	CAGTG	4 cut(s)	561, 730, 1029, 1153
Cac8I	GCNNGC	3 cut(s)	545, 778, 951
CciI	TCATGA	2 cut(s)	48, 883
CfoI	GCGC	2 cut(s)	91, 701
Cfr13I	GGNCC	1 cut(s)	460
CseI	GACGC	1 cut(s)	897
Csp6I	GTAC	2 cut(s)	995, 1041
CspCI	CAANNNNNGTGG	2 cut(s)	1018, 1053
CviAII	CATG	6 cut(s)	41, 49, 884, 1052, 1087, 1105
CviQI	GTAC	2 cut(s)	995, 1041
DdeI	CTNAG	4 cut(s)	969, 1113, 1271, 1440
DpnI	GATC	4 cut(s)	299, 379, 740, 1314
DpnII	GATC	4 cut(s)	297, 377, 738, 1312
Eam1104I	CTCTTC	1 cut(s)	150
EarI	CTCTTC	1 cut(s)	150
Ecl136II	GAGCTC	1 cut(s)	469
Eco130I	CCWWGG	1 cut(s)	237
Eco147I	AGGCCT	1 cut(s)	1331
Eco24I	GRGCYC	1 cut(s)	471
Eco31I	GGTCTC	2 cut(s)	506, 1225
Eco32I	GATATC	3 cut(s)	823, 1075, 1424
Eco47III	AGCGCT	1 cut(s)	700
Eco53kI	GAGCTC	1 cut(s)	469
Eco57I	CTGAAG	1 cut(s)	1081
EcoICRI	GAGCTC	1 cut(s)	469
EcoRV	GATATC	3 cut(s)	823, 1075, 1424
EcoT14I	CCWWGG	1 cut(s)	237
EcoT38I	GRGCYC	1 cut(s)	471
ErhI	CCWWGG	1 cut(s)	237
FaeI	CATG	6 cut(s)	44, 52, 887, 1055, 1090, 1108
FaqI	GGGAC	2 cut(s)	461, 693
FatI	CATG	6 cut(s)	40, 48, 883, 1051, 1086, 1104
Fnu4HI	GCNGC	3 cut(s)	75, 234, 388
FriOI	GRGCYC	1 cut(s)	471
Fsp4HI	GCNGC	3 cut(s)	75, 234, 388
FspBI	CTAG	1 cut(s)	219
GlaI	GCGC	2 cut(s)	90, 700
GluI	GCNGC	3 cut(s)	75, 234, 388
GsuI	CTGGAG	2 cut(s)	841, 933
HaeII	RGCGCY	1 cut(s)	702
HaeIII	GGCC	2 cut(s)	462, 1331
HapII	CCGG	1 cut(s)	1427
HgaI	GACGC	1 cut(s)	897
HhaI	GCGC	2 cut(s)	91, 701
Hin1I	GRCGYC	1 cut(s)	908
Hin1II	CATG	6 cut(s)	44, 52, 887, 1055, 1090, 1108
Hin6I	GCGC	2 cut(s)	89, 699
HinP1I	GCGC	2 cut(s)	89, 699
HinfI	GANTC	5 cut(s)	555, 880, 1048, 1092, 1360
HpaII	CCGG	1 cut(s)	1427
HphI	GGTGA	4 cut(s)	16, 1082, 1240, 1400
Hpy166II	GTNNAC	3 cut(s)	655, 727, 986
Hpy188I	TCNGA	6 cut(s)	315, 552, 603, 690, 1100, 1131
Hpy188III	TCNNGA	4 cut(s)	49, 884, 912, 1340
Hpy8I	GTNNAC	3 cut(s)	655, 727, 986
HpyAV	CCTTC	9 cut(s)	333, 344, 478, 485, 756, 793, 1210, 1288, 1447
HpyCH4III	ACNGT	3 cut(s)	406, 1138, 1148
HpyCH4IV	ACGT	1 cut(s)	944
HpyF10VI	GCNNNNNNNGC	1 cut(s)	71
HpyF3I	CTNAG	4 cut(s)	969, 1113, 1271, 1440
HpySE526I	ACGT	1 cut(s)	944
Hsp92I	GRCGYC	1 cut(s)	908
Hsp92II	CATG	6 cut(s)	44, 52, 887, 1055, 1090, 1108
HspAI	GCGC	2 cut(s)	89, 699
Kzo9I	GATC	4 cut(s)	297, 377, 738, 1312
LmnI	GCTCC	2 cut(s)	82, 400
Lsp1109I	GCAGC	2 cut(s)	61, 220
LweI	GCATC	4 cut(s)	220, 556, 706, 763
MaeI	CTAG	1 cut(s)	219
MaeII	ACGT	1 cut(s)	944
MaeIII	GTNAC	3 cut(s)	406, 616, 931
MalI	GATC	4 cut(s)	299, 379, 740, 1314
MboI	GATC	4 cut(s)	297, 377, 738, 1312
MboII	GAAGA	8 cut(s)	64, 160, 167, 359, 500, 568, 1087, 1186
MfeI	CAATTG	1 cut(s)	415
MhlI	GDGCHC	3 cut(s)	397, 471, 988
MluCI	AATT	4 cut(s)	415, 1159, 1282, 1304
MlyI	GAGTC	1 cut(s)	549
MslI	CAYNNNNRTG	1 cut(s)	53
MspA1I	CMGCKG	1 cut(s)	284
MspCI	CTTAAG	1 cut(s)	958
MspI	CCGG	1 cut(s)	1427
MunI	CAATTG	1 cut(s)	415
MwoI	GCNNNNNNNGC	1 cut(s)	71
NdeII	GATC	4 cut(s)	297, 377, 738, 1312
NlaIII	CATG	6 cut(s)	44, 52, 887, 1055, 1090, 1108
NlaIV	GGNNCC	1 cut(s)	461
NmuCI	GTSAC	1 cut(s)	616
NspV	TTCGAA	2 cut(s)	791, 1433
PagI	TCATGA	2 cut(s)	48, 883
PceI	AGGCCT	1 cut(s)	1331
PfeI	GAWTC	4 cut(s)	880, 1048, 1092, 1360
PkrI	GCNGC	3 cut(s)	76, 235, 389
PleI	GAGTC	1 cut(s)	549
PpsI	GAGTC	1 cut(s)	549
PshBI	ATTAAT	2 cut(s)	201, 846
PsiI	TTATAA	1 cut(s)	182
Psp124BI	GAGCTC	1 cut(s)	471
PspN4I	GGNNCC	1 cut(s)	461
PspPI	GGNCC	1 cut(s)	460
RsaI	GTAC	2 cut(s)	996, 1042
RsaNI	GTAC	2 cut(s)	995, 1041
RseI	CAYNNNNRTG	1 cut(s)	53
SacI	GAGCTC	1 cut(s)	471
SatI	GCNGC	3 cut(s)	75, 234, 388
Sau3AI	GATC	4 cut(s)	297, 377, 738, 1312
Sau96I	GGNCC	1 cut(s)	460
ScaI	AGTACT	1 cut(s)	996
SchI	GAGTC	1 cut(s)	549
SduI	GDGCHC	3 cut(s)	397, 471, 988
SfaNI	GCATC	4 cut(s)	220, 556, 706, 763
SfuI	TTCGAA	2 cut(s)	791, 1433
SmiMI	CAYNNNNRTG	1 cut(s)	53
SmlI	CTYRAG	1 cut(s)	958
SmoI	CTYRAG	1 cut(s)	958
Sse9I	AATT	4 cut(s)	415, 1159, 1282, 1304
SseBI	AGGCCT	1 cut(s)	1331
SsiI	CCGC	2 cut(s)	282, 387
SspI	AATATT	1 cut(s)	661
SspMI	CTAG	1 cut(s)	219
SstI	GAGCTC	1 cut(s)	471
StuI	AGGCCT	1 cut(s)	1331
StyI	CCWWGG	1 cut(s)	237
TaaI	ACNGT	3 cut(s)	406, 1138, 1148
TaiI	ACGT	1 cut(s)	947
TaqI	TCGA	4 cut(s)	791, 1077, 1315, 1433
TasI	AATT	4 cut(s)	415, 1159, 1282, 1304
TatI	WGTACW	1 cut(s)	994
TauI	GCSGC	1 cut(s)	390
TfiI	GAWTC	4 cut(s)	880, 1048, 1092, 1360
TscAI	CASTG	4 cut(s)	568, 737, 1036, 1153
TseFI	GTSAC	1 cut(s)	616
TseI	GCWGC	2 cut(s)	74, 233
Tsp45I	GTSAC	1 cut(s)	616
TspDTI	ATGAA	6 cut(s)	65, 497, 597, 872, 1040, 1229
TspGWI	ACGGA	1 cut(s)	69
TspRI	CASTG	4 cut(s)	568, 737, 1036, 1153
Vha464I	CTTAAG	1 cut(s)	958
VneI	GTGCAC	1 cut(s)	984
VspI	ATTAAT	2 cut(s)	201, 846
XapI	RAATTY	1 cut(s)	1159
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1454
XspI	CTAG	1 cut(s)	219
ZrmI	AGTACT	1 cut(s)	996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.