Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0000874

Physical Location & Seq

Genomic Coordinates Reverse (-)

48404888 .. 48408323

Gene Structure 3436 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG267200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGAACAGTCTATCACTCTCAGCCTCACGGCACTCAAAACCTCTCCTCCACCTTCTTAAACCGCAAACCCCAAAACCCAAATCCCCAGTCTTCATCCTCACCCACCACCTCTGCACCAATCTCAGCCCCAATCCGAACCAACCACCCCAAGACGACGACACCTTCATAACCCAAGTCCTCCAACTCCTCCAACCCAATGAGAAAGACTGGAACTTCGACCGACTCCACACCCTCCTCTTCTCCAACTCAACCTCTCCTTCCCCTCACTCTGTCTTCCACATCACTCGCCGCCTCGGCGCTCCGTCCAAAGCCATCAAATTCTTCGACTACATTTCCGAAAATGTGGGAACAACAACCCCTCCAGGCTCCAATGGTTTGCTCTCCTCCGCATTCCAGGCCGTTCTGGAGCTCACATCGCAACAACCCACATCGGAAAAGAGCCTCATTGAGCTTTACGAGATGGCGAAGAAGCGGAATGTGGCGCTTGATGTAAACGCCGCAGCTCTGCTTGTTCGGTCCCTGGGGACGGCTGGCATGGAGGACGAGGCTGTCATGGTGTTTAATGAACTCGATTCGGGGTTGAAGAACACTCATATTCGCAATGTGGTGATTAGGTTGTTGCTGAACATGGGGCGAGTTGATGATGCACTGAAGGTGCTCGACGAAATGCTTGACCCAGAAGCAAAGTCCCGGGTTGACGACTTTACCGGGGATATTGTGATTGGCTCATTGTTGAGGAAAGAGCAGAAAGGGAGGAGAGTCAGTGTGGAGGAAATTGTGGACTTGGTGTCGAAATTTGGTAAGCATGGTGTGTTTCCTAATAGCCCGATACTTACGAAATTGGTGACTGTTTTGTGTAGGAATGGGAAGGTTAGTCGCGCTTGGGATGTGTTGCATGATGTTATGGAGATGGGTGGTGCTGTAGAAGCTGCTTCTTGCAATGCGCTTTTGACAGCTTTGGGAAGAGGTAATGATTTTAAGAGGATGAGTGAGCTTATGGTGAAGATGGAAGAGGTGGGCATTAAGCCGAATCTTATTACTTTTGGTATTCTTATTAACCGTTTATGTAAGTCTGGGAGACTAGATGCTGCCTTGGAGGTGTTTGAGAAGATGAGTGCAGGAGTAAAGGGGGTTTCGGCTAAACCGGATGTGATTATCTATAACACTTTGATTGATGGACTTTGTAAAGCAGGAAGGCCAGAAGAGGGATTGCGTTTGATGGGAAAGATGAGATCGCAAGATGGGGCTCCCAATACTGTTACGTACAATTGTTTGATTGATGGTTTCAACAAAGTTGGGGACATTGAGAGGGGTCGTGAGCTCTTTGACAAAATGAAGGAGGAAGGGATACCGCTAAATGTGGTCACCCTCAATACTTTGCTTGATGGTTTGTGCAGGCATGGGAGACTCAACACCGCACTTGAGTTCTTCAAGGAGATGCAGAGGGATGGTCTGAAAGGCAATGCCGTTACTTACACTATCTTAATCACTTCCTTTTGTAATGTGAACAATATTAGCAAGGCAATGGAGTTGTTTGATCAGATGTTGAGCGCTGAATGTCCCACAGATGCAAAAGTTTATCACTGCTTGATCTCTGGTTTAAGCCTAGCCGGAAGGATGGAGGATGCCAGCTTTGTTGTCTCAAAGTTGAAGGAGGCTGGGTTCTCCATGGATAATGTTTCCTACAATGTAATGATTAATGGGTTCTCCAGTAAAAATAAGCCTGATAAGATTCATGAGATGATTGAGGAAATGGAAGCGTCTGGAGTGAAGCCTGATAGTGTGACATACAACACCTTGCTTGCCTACTTAAGCAAAGCTGGAGAATTCAAAAGTGCACATAAAGTACTTGACAGGATGAGGGATGAGGGTATTGTTCCCACTGTGGTCACTTTTGGTACATTGATTCATGCACATTGCTTGAATGGCGATATTGACAAAGCCATGAGAATCTTCAGAGACATGGGTTCTAAGTCAAAGATACCTCCGAATACTGTAATATACAATGATTTAATAAATTCCCTCTGTAAGAAGAATGATGTGGAACAAGCTCTTTATTTGATGGATGATATGAAGGATAAGGGGGTGAGACCTAATACCCAAACGTTCAATGCCTTGTTCAAAGGCCTTAGAGAGAACAATTTATTTGAGAAGGCATTTCAATTTATGGATCGAATGGTTGAACAGGCCTGTAATCCTGATTATATAACGATGGAAATTCTTACCGAATGGCTTTCCTGTGTTGGTGAAATACAGAGGTTGAGAAAGTTTGTTCAAGGATATCCAGTTTCAAAGACACAGGGCAAAGCGGCTGGTTTCAAAGCAACAGAGGGAAAAGCCACTGGTTTCAAAGCGACAGAGGCAAAGCCCGCTGGTTTCAAAGCAACAGAAGGGAAACCCGCTGGTTTCAAAGCGACAGAGGAAAAAGCCGCTGGTTTCAAAGCGACAGAAGGCAACACTAACATTATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

823

Amino Acids

90.89

Weight (kDa)

8.04

Isoelectric Point (pI)

31.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	297 - 353	1.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	311 - 357	1.3e-08	PPR repeat family
PPR_1	PF12854	340 - 372	5.4e-12	PPR repeat
PPR_1	PF12854	381 - 411	1.2e-10	PPR repeat
PPR_2	PF13041	382 - 430	2.6e-15	PPR repeat family
PPR	PF01535	386 - 412	2.2e-07	PPR repeat
TPR_24	PF23276	386 - 480	5.6e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	417 - 446	4.3e-08	PPR repeat
PPR_2	PF13041	417 - 466	2.8e-16	PPR repeat family
PPR	PF01535	420 - 450	6e-08	PPR repeat
PPR_1	PF12854	449 - 481	4.3e-12	PPR repeat
PPR_2	PF13041	453 - 501	1e-17	PPR repeat family
PPR	PF01535	455 - 485	2.7e-08	PPR repeat
PPR_1	PF12854	484 - 515	8e-10	PPR repeat
PPR_2	PF13041	488 - 534	2.5e-10	PPR repeat family
PPR	PF01535	490 - 519	4.5e-06	PPR repeat
PPR_long	PF17177	492 - 628	4.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	524 - 570	4.8e-07	PPR repeat family
TPR_24	PF23276	537 - 654	3.5e-10	Fungal tetratrico peptide repeats
PPR_2	PF13041	558 - 601	4.5e-13	PPR repeat family
PPR	PF01535	560 - 590	5.1e-06	PPR repeat
PPR_3	PF13812	582 - 638	9e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	589 - 621	1.7e-07	PPR repeat
PPR_2	PF13041	592 - 640	1.3e-14	PPR repeat family
PPR_long	PF17177	616 - 746	2.4e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	624 - 655	2.8e-11	PPR repeat
PPR_2	PF13041	627 - 664	7.8e-07	PPR repeat family
PPR_3	PF13812	629 - 674	1e-05	Pentatricopeptide repeat domain
PPR	PF01535	630 - 657	9e-06	PPR repeat
PPR_3	PF13812	650 - 708	5.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	661 - 692	1.6e-08	PPR repeat
PPR_2	PF13041	663 - 710	4.1e-17	PPR repeat family
PPR	PF01535	666 - 696	4.4e-06	PPR repeat
Decapeptide	PF25296	769 - 817	1.7e-09	Decapeptide repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000874)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61520 AT5G28370 AT5G28460
fragaria_vesca	FvH4_5g03011 FvH4_5g03011 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_5g32150 FvH4_7g15360
malus_domestica	MD01G1071400.v1.1 MD07G1131300.v1.1
prunus_persica	Prupe.2G177500_v2.0.a1
pyrus_communis	pycom01g10040 pycom04g02360 pycom07g12830
rosa_chinensis	RchiOBHm_Chr1g0357681 RchiOBHm_Chr3g0468501 RchiOBHm_Chr5g0017031 RchiOBHm_Chr7g0228211 RchiOBHm_Chr7g0228281 RchiOBHm_Chr7g0228291
rosa_laevigata	RLG00000001577 RLG00000005833 RLG00000009015 RLG00000009124 RLG00000012161 RLG00000028007 RLG00000028484
rosa_multiflora	Rmu_sc0001062.1_g000004 Rmu_sc0001626.1_g000014 Rmu_sc0001626.1_g000015 Rmu_sc0002582.1_g000009 Rmu_sc0003490.1_g000025 Rmu_sc0003490.1_g000026 Rmu_sc0004842.1_g000011 Rmu_sc0004842.1_g000012 Rmu_sc0004842.1_g000020 Rmu_ssc0000425.1_g000002
rosa_roxburghii	Rroxscaffold_3G00231300 Rroxscaffold_4G00298600 Rroxscaffold_6G00429570
rosa_rugosa	Rorug01G0257700 Rorug02G0051500 Rorug02G0572600 Rorug07G0246900.1 Rorug07G0247000 Rorug07G0247000
rosa_samantha	Rh1AG271700 Rh1BG238800 Rh1CG255600 Rh1DG267200 Rh3AG143700 Rh3BG166200 Rh3BG166300 Rh5AG043600 Rh7AG400900 Rh7AG401200 Rh7BG382400 Rh7CG420000 Rh7DG396900 Rh7DG397300
rosa_wichuraiana	Rw1G024230 Rw7G033300 Rw7G033330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	548
AccII	CGCG	1 cut(s)	879
AclI	AACGTT	1 cut(s)	2105
AclWI	GGATC	1 cut(s)	2178
AcsI	RAATTY	5 cut(s)	317, 794, 1826, 2017, 2217
AcuI	CTGAAG	2 cut(s)	671, 1939
AfaI	GTAC	3 cut(s)	1265, 1848, 1900
AfeI	AGCGCT	1 cut(s)	1552
AfiI	CCNNNNNNNGG	2 cut(s)	526, 1205
AflII	CTTAAG	1 cut(s)	1810
AhdI	GACNNNNNGTC	1 cut(s)	787
AjnI	CCWGG	3 cut(s)	361, 393, 519
AloI	GAACNNNNNNTCC	2 cut(s)	1646, 1678
Alw21I	GWGCWC	4 cut(s)	411, 660, 1323, 1840
Alw26I	GTCTC	5 cut(s)	1072, 1399, 1645, 1953, 2083
Alw44I	GTGCAC	1 cut(s)	1836
AlwI	GGATC	1 cut(s)	2178
Ama87I	CYCGRG	1 cut(s)	690
Aor51HI	AGCGCT	1 cut(s)	1552
AoxI	GGCC	4 cut(s)	396, 1196, 2125, 2187
ApaLI	GTGCAC	1 cut(s)	1836
ApeKI	GCWGC	3 cut(s)	500, 929, 1088
ApoI	RAATTY	5 cut(s)	317, 794, 1826, 2017, 2217
ArsI	GACNNNNNNTTYG	4 cut(s)	72, 104, 197, 229
AseI	ATTAAT	1 cut(s)	1698
AspLEI	GCGC	5 cut(s)	299, 484, 881, 946, 1553
AspS9I	GGNCC	1 cut(s)	516
AsuC2I	CCSGG	3 cut(s)	691, 692, 709
AsuHPI	GGTGA	7 cut(s)	91, 619, 856, 1012, 1357, 2098, 2258
AvaI	CYCGRG	1 cut(s)	690
AvaII	GGWCC	1 cut(s)	516
BaeGI	GKGCMC	1 cut(s)	1840
BanII	GRGCYC	3 cut(s)	411, 1249, 1323
BbsI	GAAGAC	2 cut(s)	82, 265
Bbv12I	GWGCWC	4 cut(s)	411, 660, 1323, 1840
BbvI	GCAGC	3 cut(s)	512, 916, 1075
BceAI	ACGGC	4 cut(s)	44, 383, 543, 1451
BciT130I	CCWGG	3 cut(s)	363, 395, 521
BciVI	GTATCC	1 cut(s)	1341
BclI	TGATCA	1 cut(s)	1537
BcnI	CCSGG	3 cut(s)	691, 692, 709
BcoDI	GTCTC	5 cut(s)	1072, 1399, 1645, 1953, 2083
BfaI	CTAG	2 cut(s)	1082, 1607
BfmI	CTRYAG	1 cut(s)	921
BfoI	RGCGCY	3 cut(s)	300, 485, 1554
BfrI	CTTAAG	1 cut(s)	1810
BfuI	GTATCC	1 cut(s)	1341
BglI	GCCNNNNNGGC	1 cut(s)	294
BisI	GCNGC	7 cut(s)	289, 498, 501, 930, 1089, 2310, 2430
BlsI	GCNGC	7 cut(s)	290, 499, 502, 931, 1090, 2311, 2431
BmcAI	AGTACT	1 cut(s)	1848
Bme1390I	CCNGG	6 cut(s)	363, 395, 521, 691, 692, 709
Bme18I	GGWCC	1 cut(s)	516
BmeRI	GACNNNNNGTC	1 cut(s)	787
BmeT110I	CYCGRG	1 cut(s)	690
BmgT120I	GGNCC	1 cut(s)	516
BmiI	GGNNCC	3 cut(s)	367, 518, 1248
BmrFI	CCNGG	6 cut(s)	363, 395, 521, 691, 692, 709
BmrI	ACTGGG	1 cut(s)	80
BmsI	GCATC	5 cut(s)	634, 1075, 1428, 1558, 1615
BmuI	ACTGGG	1 cut(s)	80
BpiI	GAAGAC	2 cut(s)	82, 265
BpmI	CTGGAG	5 cut(s)	345, 425, 1693, 1785, 1842
BpuEI	CTTGAG	1 cut(s)	1442
BpuMI	CCSGG	3 cut(s)	691, 692, 709
BsaAI	YACGTR	1 cut(s)	1263
BsaI	GGTCTC	1 cut(s)	2083
BsaJI	CCNNGG	7 cut(s)	292, 519, 520, 690, 708, 1092, 1668
BsaWI	WCCGGW	1 cut(s)	1144
BsaXI	ACNNNNNCTCC	8 cut(s)	30, 60, 343, 373, 748, 778, 1646, 1676
Bsc4I	CCNNNNNNNGG	2 cut(s)	526, 1205
Bse1I	ACTGG	5 cut(s)	86, 212, 1710, 2285, 2347
Bse3DI	GCAATG	5 cut(s)	607, 946, 1468, 1530, 1915
BseBI	CCWGG	3 cut(s)	363, 395, 521
BseDI	CCNNGG	7 cut(s)	292, 519, 520, 690, 708, 1092, 1668
BseLI	CCNNNNNNNGG	2 cut(s)	526, 1205
BseMI	GCAATG	5 cut(s)	607, 946, 1468, 1530, 1915
BseMII	CTCAG	2 cut(s)	33, 136
BseNI	ACTGG	5 cut(s)	86, 212, 1710, 2285, 2347
BseRI	GAGGAG	5 cut(s)	35, 176, 224, 373, 769
BseSI	GKGCMC	1 cut(s)	1840
BseXI	GCAGC	3 cut(s)	512, 916, 1075
BseYI	CCCAGC	1 cut(s)	1658
BsgI	GTGCAG	3 cut(s)	97, 1137, 1414
Bsh1236I	CGCG	1 cut(s)	879
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	4 cut(s)	398, 1198, 2127, 2189
BsiEI	CGRYCG	1 cut(s)	220
BsiHKAI	GWGCWC	4 cut(s)	411, 660, 1323, 1840
BsiHKCI	CYCGRG	1 cut(s)	690
BsiSI	CCGG	4 cut(s)	691, 708, 1145, 1611
BslFI	GGGAC	5 cut(s)	502, 538, 673, 1313, 1545
BslI	CCNNNNNNNGG	2 cut(s)	526, 1205
BsmAI	GTCTC	5 cut(s)	1072, 1399, 1645, 1953, 2083
BsmFI	GGGAC	5 cut(s)	502, 538, 673, 1313, 1545
BsmI	GAATGC	1 cut(s)	389
BsnI	GGCC	4 cut(s)	398, 1198, 2127, 2189
Bso31I	GGTCTC	1 cut(s)	2083
BsoBI	CYCGRG	1 cut(s)	690
Bsp1286I	GDGCHC	5 cut(s)	411, 660, 1249, 1323, 1840
Bsp143I	GATC	4 cut(s)	1232, 1537, 1590, 2170
Bsp19I	CCATGG	1 cut(s)	1668
BspANI	GGCC	4 cut(s)	398, 1198, 2127, 2189
BspCNI	CTCAG	2 cut(s)	32, 135
BspFNI	CGCG	1 cut(s)	879
BspHI	TCATGA	1 cut(s)	1735
BspLI	GGNNCC	3 cut(s)	367, 518, 1248
BspPI	GGATC	1 cut(s)	2178
BspTI	CTTAAG	1 cut(s)	1810
BspTNI	GGTCTC	1 cut(s)	2083
BsrDI	GCAATG	5 cut(s)	607, 946, 1468, 1530, 1915
BsrI	ACTGG	5 cut(s)	86, 212, 1710, 2285, 2347
BssECI	CCNNGG	7 cut(s)	292, 519, 520, 690, 708, 1092, 1668
BssMI	GATC	4 cut(s)	1232, 1537, 1590, 2170
BssT1I	CCWWGG	2 cut(s)	1092, 1668
Bst2UI	CCWGG	3 cut(s)	363, 395, 521
Bst4CI	ACNGT	6 cut(s)	8, 850, 1061, 1258, 1885, 1996
Bst6I	CTCTTC	4 cut(s)	242, 958, 1005, 1197
BstAFI	CTTAAG	1 cut(s)	1810
BstBAI	YACGTR	1 cut(s)	1263
BstC8I	GCNNGC	5 cut(s)	532, 1397, 1630, 1803, 2370
BstDEI	CTNAG	4 cut(s)	19, 122, 1971, 2129
BstDSI	CCRYGG	1 cut(s)	1668
BstEII	GGTNACC	1 cut(s)	1363
BstFNI	CGCG	1 cut(s)	879
BstH2I	RGCGCY	3 cut(s)	300, 485, 1554
BstHHI	GCGC	5 cut(s)	299, 484, 881, 946, 1553
BstKTI	GATC	4 cut(s)	1235, 1540, 1593, 2173
BstMAI	GTCTC	5 cut(s)	1072, 1399, 1645, 1953, 2083
BstMBI	GATC	4 cut(s)	1232, 1537, 1590, 2170
BstMCI	CGRYCG	1 cut(s)	220
BstMWI	GCNNNNNNNGC	7 cut(s)	294, 395, 415, 926, 1926, 2360, 2369
BstNI	CCWGG	3 cut(s)	363, 395, 521
BstPI	GGTNACC	1 cut(s)	1363
BstSCI	CCNGG	6 cut(s)	361, 393, 519, 689, 690, 707
BstSFI	CTRYAG	1 cut(s)	921
BstSLI	GKGCMC	1 cut(s)	1840
BstSNI	TACGTA	1 cut(s)	1263
BstUI	CGCG	1 cut(s)	879
BstV1I	GCAGC	3 cut(s)	512, 916, 1075
BstV2I	GAAGAC	2 cut(s)	82, 265
BsuI	GTATCC	1 cut(s)	1341
BsuRI	GGCC	4 cut(s)	398, 1198, 2127, 2189
BtgI	CCRYGG	1 cut(s)	1668
BtgZI	GCGATG	1 cut(s)	399
BtsI	GCAGTG	1 cut(s)	1582
BtsIMutI	CAGTG	5 cut(s)	647, 769, 1582, 1881, 2340
Cac8I	GCNNGC	5 cut(s)	532, 1397, 1630, 1803, 2370
CciI	TCATGA	1 cut(s)	1735
CfoI	GCGC	5 cut(s)	299, 484, 881, 946, 1553
Cfr13I	GGNCC	1 cut(s)	516
Cfr9I	CCCGGG	1 cut(s)	690
CseI	GACGC	1 cut(s)	1749
Csp6I	GTAC	3 cut(s)	1264, 1847, 1899
CviQI	GTAC	3 cut(s)	1264, 1847, 1899
DdeI	CTNAG	4 cut(s)	19, 122, 1971, 2129
DpnI	GATC	4 cut(s)	1234, 1539, 1592, 2172
DpnII	GATC	4 cut(s)	1232, 1537, 1590, 2170
DrdI	GACNNNNNNGTC	1 cut(s)	548
DriI	GACNNNNNGTC	1 cut(s)	787
DseDI	GACNNNNNNGTC	1 cut(s)	548
Eam1104I	CTCTTC	4 cut(s)	242, 958, 1005, 1197
Eam1105I	GACNNNNNGTC	1 cut(s)	787
EarI	CTCTTC	4 cut(s)	242, 958, 1005, 1197
Ecl136II	GAGCTC	2 cut(s)	409, 1321
Eco105I	TACGTA	1 cut(s)	1263
Eco130I	CCWWGG	2 cut(s)	1092, 1668
Eco147I	AGGCCT	2 cut(s)	2127, 2189
Eco24I	GRGCYC	3 cut(s)	411, 1249, 1323
Eco31I	GGTCTC	1 cut(s)	2083
Eco32I	GATATC	1 cut(s)	2282
Eco47I	GGWCC	1 cut(s)	516
Eco47III	AGCGCT	1 cut(s)	1552
Eco53kI	GAGCTC	2 cut(s)	409, 1321
Eco57I	CTGAAG	2 cut(s)	671, 1939
Eco88I	CYCGRG	1 cut(s)	690
Eco91I	GGTNACC	1 cut(s)	1363
EcoICRI	GAGCTC	2 cut(s)	409, 1321
EcoO65I	GGTNACC	1 cut(s)	1363
EcoRI	GAATTC	1 cut(s)	1826
EcoRII	CCWGG	3 cut(s)	361, 393, 519
EcoRV	GATATC	1 cut(s)	2282
EcoT14I	CCWWGG	2 cut(s)	1092, 1668
EcoT38I	GRGCYC	3 cut(s)	411, 1249, 1323
ErhI	CCWWGG	2 cut(s)	1092, 1668
FaqI	GGGAC	5 cut(s)	502, 538, 673, 1313, 1545
FauI	CCCGC	2 cut(s)	2377, 2407
FbaI	TGATCA	1 cut(s)	1537
Fnu4HI	GCNGC	7 cut(s)	289, 498, 501, 930, 1089, 2310, 2430
FriOI	GRGCYC	3 cut(s)	411, 1249, 1323
Fsp4HI	GCNGC	7 cut(s)	289, 498, 501, 930, 1089, 2310, 2430
FspBI	CTAG	2 cut(s)	1082, 1607
GlaI	GCGC	5 cut(s)	298, 483, 880, 945, 1552
GluI	GCNGC	7 cut(s)	289, 498, 501, 930, 1089, 2310, 2430
GsaI	CCCAGC	1 cut(s)	1662
GsuI	CTGGAG	5 cut(s)	345, 425, 1693, 1785, 1842
HaeII	RGCGCY	3 cut(s)	300, 485, 1554
HaeIII	GGCC	4 cut(s)	398, 1198, 2127, 2189
HapII	CCGG	4 cut(s)	691, 708, 1145, 1611
HgaI	GACGC	1 cut(s)	1749
HhaI	GCGC	5 cut(s)	299, 484, 881, 946, 1553
Hin6I	GCGC	5 cut(s)	297, 482, 879, 944, 1551
HinP1I	GCGC	5 cut(s)	297, 482, 879, 944, 1551
HincII	GTYRAC	1 cut(s)	697
HindII	GTYRAC	1 cut(s)	697
HinfI	GANTC	8 cut(s)	222, 572, 759, 1030, 1407, 1732, 1906, 1950
HpaII	CCGG	4 cut(s)	691, 708, 1145, 1611
HphI	GGTGA	7 cut(s)	91, 619, 856, 1012, 1357, 2098, 2258
Hpy166II	GTNNAC	5 cut(s)	493, 697, 781, 1507, 1838
Hpy188I	TCNGA	7 cut(s)	135, 337, 433, 1455, 1542, 1958, 1989
Hpy188III	TCNNGA	5 cut(s)	404, 1316, 1736, 1764, 2198
Hpy8I	GTNNAC	5 cut(s)	493, 697, 781, 1507, 1838
Hpy99I	CGWCG	2 cut(s)	158, 665
HpyCH4III	ACNGT	6 cut(s)	8, 850, 1061, 1258, 1885, 1996
HpyCH4IV	ACGT	2 cut(s)	1262, 2105
HpyF10VI	GCNNNNNNNGC	7 cut(s)	294, 395, 415, 926, 1926, 2360, 2369
HpyF3I	CTNAG	4 cut(s)	19, 122, 1971, 2129
HpySE526I	ACGT	2 cut(s)	1262, 2105
HspAI	GCGC	5 cut(s)	297, 482, 879, 944, 1551
Ksp22I	TGATCA	1 cut(s)	1537
Kzo9I	GATC	4 cut(s)	1232, 1537, 1590, 2170
LmnI	GCTCC	4 cut(s)	304, 371, 406, 1252
Lsp1109I	GCAGC	3 cut(s)	512, 916, 1075
LweI	GCATC	5 cut(s)	634, 1075, 1428, 1558, 1615
MaeI	CTAG	2 cut(s)	1082, 1607
MaeII	ACGT	2 cut(s)	1262, 2105
MaeIII	GTNAC	6 cut(s)	844, 1258, 1363, 1468, 1783, 1888
MalI	GATC	4 cut(s)	1234, 1539, 1592, 2172
MboI	GATC	4 cut(s)	1232, 1537, 1590, 2170
MfeI	CAATTG	1 cut(s)	1267
MhlI	GDGCHC	5 cut(s)	411, 660, 1249, 1323, 1840
MlyI	GAGTC	3 cut(s)	216, 768, 1401
MmeI	TCCRAC	3 cut(s)	205, 214, 267
MspA1I	CMGCKG	3 cut(s)	2372, 2402, 2432
MspCI	CTTAAG	1 cut(s)	1810
MspI	CCGG	4 cut(s)	691, 708, 1145, 1611
MspR9I	CCNGG	6 cut(s)	363, 395, 521, 691, 692, 709
MunI	CAATTG	1 cut(s)	1267
Mva1269I	GAATGC	1 cut(s)	389
MvaI	CCWGG	3 cut(s)	363, 395, 521
MvnI	CGCG	1 cut(s)	879
MwoI	GCNNNNNNNGC	7 cut(s)	294, 395, 415, 926, 1926, 2360, 2369
NciI	CCSGG	3 cut(s)	691, 692, 709
NcoI	CCATGG	1 cut(s)	1668
NdeII	GATC	4 cut(s)	1232, 1537, 1590, 2170
NlaIV	GGNNCC	3 cut(s)	367, 518, 1248
NmeAIII	GCCGAG	1 cut(s)	273
NmuCI	GTSAC	4 cut(s)	844, 1363, 1783, 1888
PagI	TCATGA	1 cut(s)	1735
PasI	CCCWGGG	1 cut(s)	520
PceI	AGGCCT	2 cut(s)	2127, 2189
PctI	GAATGC	1 cut(s)	389
PfeI	GAWTC	5 cut(s)	572, 1030, 1732, 1906, 1950
PkrI	GCNGC	7 cut(s)	290, 499, 502, 931, 1090, 2311, 2431
PleI	GAGTC	3 cut(s)	216, 767, 1401
PpsI	GAGTC	3 cut(s)	216, 767, 1401
Ppu21I	YACGTR	1 cut(s)	1263
PshBI	ATTAAT	1 cut(s)	1698
Psp124BI	GAGCTC	2 cut(s)	411, 1323
Psp1406I	AACGTT	1 cut(s)	2105
Psp6I	CCWGG	3 cut(s)	361, 393, 519
PspEI	GGTNACC	1 cut(s)	1363
PspFI	CCCAGC	1 cut(s)	1658
PspGI	CCWGG	3 cut(s)	361, 393, 519
PspN4I	GGNNCC	3 cut(s)	367, 518, 1248
PspPI	GGNCC	1 cut(s)	516
RsaI	GTAC	3 cut(s)	1265, 1848, 1900
RsaNI	GTAC	3 cut(s)	1264, 1847, 1899
SacI	GAGCTC	2 cut(s)	411, 1323
SatI	GCNGC	7 cut(s)	289, 498, 501, 930, 1089, 2310, 2430
Sau3AI	GATC	4 cut(s)	1232, 1537, 1590, 2170
Sau96I	GGNCC	1 cut(s)	516
ScaI	AGTACT	1 cut(s)	1848
SchI	GAGTC	3 cut(s)	216, 768, 1401
ScrFI	CCNGG	6 cut(s)	363, 395, 521, 691, 692, 709
SduI	GDGCHC	5 cut(s)	411, 660, 1249, 1323, 1840
SfaNI	GCATC	5 cut(s)	634, 1075, 1428, 1558, 1615
SfcI	CTRYAG	1 cut(s)	921
SinI	GGWCC	1 cut(s)	516
SmaI	CCCGGG	1 cut(s)	692
SmlI	CTYRAG	2 cut(s)	1421, 1810
SmoI	CTYRAG	2 cut(s)	1421, 1810
SnaBI	TACGTA	1 cut(s)	1263
SseBI	AGGCCT	2 cut(s)	2127, 2189
SspI	AATATT	1 cut(s)	1513
SspMI	CTAG	2 cut(s)	1082, 1607
SstI	GAGCTC	2 cut(s)	411, 1323
StuI	AGGCCT	2 cut(s)	2127, 2189
StyD4I	CCNGG	6 cut(s)	361, 393, 519, 689, 690, 707
StyI	CCWWGG	2 cut(s)	1092, 1668
TaaI	ACNGT	6 cut(s)	8, 850, 1061, 1258, 1885, 1996
TaiI	ACGT	2 cut(s)	1265, 2108
TaqI	TCGA	6 cut(s)	216, 324, 570, 660, 791, 2173
TaqII	GACCGA	2 cut(s)	234, 504
TatI	WGTACW	1 cut(s)	1846
TauI	GCSGC	4 cut(s)	291, 500, 2312, 2432
TfiI	GAWTC	5 cut(s)	572, 1030, 1732, 1906, 1950
TscAI	CASTG	5 cut(s)	654, 769, 1589, 1888, 2347
TseFI	GTSAC	4 cut(s)	844, 1363, 1783, 1888
TseI	GCWGC	3 cut(s)	500, 929, 1088
Tsp45I	GTSAC	4 cut(s)	844, 1363, 1783, 1888
TspDTI	ATGAA	8 cut(s)	17, 82, 154, 579, 1349, 1724, 1898, 2087
TspGWI	ACGGA	1 cut(s)	291
TspMI	CCCGGG	1 cut(s)	690
TspRI	CASTG	5 cut(s)	654, 769, 1589, 1888, 2347
Vha464I	CTTAAG	1 cut(s)	1810
VneI	GTGCAC	1 cut(s)	1836
VpaK11BI	GGWCC	1 cut(s)	516
VspI	ATTAAT	1 cut(s)	1698
XapI	RAATTY	5 cut(s)	317, 794, 1826, 2017, 2217
XmaI	CCCGGG	1 cut(s)	690
XspI	CTAG	2 cut(s)	1082, 1607
ZrmI	AGTACT	1 cut(s)	1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.