MD08G1230800.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001187
Physical Location & Seq
Reverse (-)
29625015 .. 29626302
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1230800.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGAGCTCCTCCAAATTGTTACTAATGTACTTTCTACAGCTACTTGTTGGTTTTGCTACCACCTTGGTGATGGTGCAAGGCCAAGGCCAACTTACGCGCGTTGGATTCTATTCGCGTACATGTCCCACGGCAGAATCTATTGTCAAACAAACAGTTCAAACTCATTTCAACTCCAACCCTTCCGTTGCTCCTGGCTTGTTAAGGATGCACTTCCATGACTGCTTTGTCCAAGGTTGTGATGCTTCTGTCCTTCTTGACGGCCCTAATACTGAGAAAACCGCCGGGCCCAACCTCGGTTTAAGAGGTTCGGAAGTCATTGACGATGCAAAGACCAAGCTTGAAGCTGCATGTCCCGGGGTTGTTTCCTGTGCAGATATTCTCGCTCTTGCGGCCCGTGATTCCGTGGTTCTGGCTGCTCCCAAGGGATTCACTTGGAATGTGCCTACTGGAAGAAGAGATGGACGAGTTTCGCTGGCAACTGAGACTACTAATTTGCCTGGCTTTAGAGATTCCATTGATGTACAAAAGGCCAAGTTCCAAGATTTAGGTCTCAACACTCAAGATCTTGTCACGCTAGTTGGCGCACACACCATTGGCACCTCAGCTTGCCAGTTCTTCAGGTACAGACTGTATAACTTCACCACCACCGGAAATGGCGCTGACGAAACAATCAACCCTTTATTCCTTGCTCAACTACGATCAATTTGCCCTGAAAATGGTGACGCGGCCAGGCGTGTTGATTTAGACACGGGCAGCGCAAGTCGATTCGAAGCAACTTTCTTCACAAACTTGAAGAATGGTCGTGGGATACTTGAGTCGGATCAGAAGTTGTGGACCGACGCCACCACTAAAAGTTTTGTCCAACGGTTTTTGGGCGTGAGAGGCTTACAAGCATTGAATTTCAACCTCGAGTTCGGAAAGTCGATGGTAAAAATGAGTAACATCGGTGTAAAATCTGCCACAAATGGTGAAATCCGCCGCATTTGTTCTGCAGTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

334

Amino Acids

36.08

Weight (kDa)

8.78

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 290 7.2e-83 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 596
AccII CGCG 4 cut(s) 97, 99, 115, 725
AciI CCGC 5 cut(s) 279, 389, 725, 976, 979
AclWI GGATC 1 cut(s) 828
AcoI YGGCCR 1 cut(s) 726
AcsI RAATTY 1 cut(s) 898
AcuI CTGAAG 1 cut(s) 601
AcyI GRCGYC 1 cut(s) 840
AfaI GTAC 4 cut(s) 29, 118, 522, 623
AfiI CCNNNNNNNGG 2 cut(s) 293, 716
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 6 cut(s) 158, 169, 341, 793, 898, 904
AjnI CCWGG 3 cut(s) 190, 496, 728
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 5 cut(s) 6, 40, 337, 344, 605
AluI AGCT 5 cut(s) 6, 40, 337, 344, 605
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 2 cut(s) 476, 554
AlwI GGATC 1 cut(s) 828
Ama87I CYCGRG 2 cut(s) 353, 908
AoxI GGCC 7 cut(s) 79, 85, 259, 284, 390, 528, 726
ApaI GGGCCC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 344, 413, 753
ApoI RAATTY 1 cut(s) 898
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AspLEI GCGC 4 cut(s) 99, 584, 659, 758
AspS9I GGNCC 5 cut(s) 260, 284, 285, 391, 834
AsuC2I CCSGG 3 cut(s) 283, 354, 355
AsuHPI GGTGA 4 cut(s) 79, 631, 731, 980
AsuII TTCGAA 1 cut(s) 768
AvaI CYCGRG 2 cut(s) 353, 908
AvaII GGWCC 1 cut(s) 834
BaeGI GKGCMC 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 800, 833
BanI GGYRCC 1 cut(s) 596
BanII GRGCYC 2 cut(s) 8, 288
Bbv12I GWGCWC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 601
BbvI GCAGC 3 cut(s) 331, 400, 765
BccI CCATC 3 cut(s) 64, 452, 919
BceAI ACGGC 2 cut(s) 144, 274
BcgI CGANNNNNNTGC 2 cut(s) 687, 721
BciT130I CCWGG 3 cut(s) 192, 498, 730
BciVI GTATCC 1 cut(s) 801
BcnI CCSGG 3 cut(s) 283, 354, 355
BcoDI GTCTC 2 cut(s) 476, 554
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 2 cut(s) 35, 990
BfoI RGCGCY 1 cut(s) 660
BfuI GTATCC 1 cut(s) 801
BglII AGATCT 1 cut(s) 562
BisI GCNGC 6 cut(s) 345, 390, 414, 726, 754, 979
BlsI GCNGC 6 cut(s) 346, 391, 415, 727, 755, 980
Bme1390I CCNGG 6 cut(s) 192, 283, 354, 355, 498, 730
Bme18I GGWCC 1 cut(s) 834
BmeT110I CYCGRG 2 cut(s) 353, 908
BmgT120I GGNCC 5 cut(s) 260, 284, 285, 391, 834
BmiI GGNNCC 2 cut(s) 286, 598
BmrFI CCNGG 6 cut(s) 192, 283, 354, 355, 498, 730
BmsI GCATC 3 cut(s) 195, 229, 313
Bpu10I CCTNAGC 1 cut(s) 601
Bpu14I TTCGAA 1 cut(s) 768
BpuEI CTTGAG 2 cut(s) 543, 833
BpuMI CCSGG 3 cut(s) 283, 354, 355
BsaHI GRCGYC 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 9 cut(s) 63, 82, 126, 229, 292, 353, 354, 402, 420
BsaWI WCCGGW 1 cut(s) 647
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 716
Bse1I ACTGG 2 cut(s) 451, 610
BseBI CCWGG 3 cut(s) 192, 498, 730
BseDI CCNNGG 9 cut(s) 63, 82, 126, 229, 292, 353, 354, 402, 420
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 293, 716
BseMII CTCAG 3 cut(s) 261, 471, 615
BseNI ACTGG 2 cut(s) 451, 610
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 3 cut(s) 331, 400, 765
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 4 cut(s) 97, 99, 115, 725
BshFI GGCC 7 cut(s) 81, 87, 261, 286, 392, 530, 728
BshNI GGYRCC 1 cut(s) 596
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 353, 908
BsiSI CCGG 3 cut(s) 282, 354, 648
BslFI GGGAC 2 cut(s) 108, 336
BslI CCNNNNNNNGG 2 cut(s) 293, 716
BsmAI GTCTC 2 cut(s) 476, 554
BsmFI GGGAC 2 cut(s) 108, 336
BsnI GGCC 7 cut(s) 81, 87, 261, 286, 392, 530, 728
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 2 cut(s) 353, 908
Bsp119I TTCGAA 1 cut(s) 768
Bsp120I GGGCCC 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 8, 288
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 3 cut(s) 562, 698, 820
BspACI CCGC 5 cut(s) 279, 389, 725, 976, 979
BspANI GGCC 7 cut(s) 81, 87, 261, 286, 392, 530, 728
BspCNI CTCAG 3 cut(s) 262, 472, 614
BspFNI CGCG 4 cut(s) 97, 99, 115, 725
BspLI GGNNCC 2 cut(s) 286, 598
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 768
BspT107I GGYRCC 1 cut(s) 596
BspTNI GGTCTC 1 cut(s) 554
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 2 cut(s) 451, 610
BssECI CCNNGG 9 cut(s) 63, 82, 126, 229, 292, 353, 354, 402, 420
BssMI GATC 3 cut(s) 562, 698, 820
BssNI GRCGYC 1 cut(s) 840
BssT1I CCWWGG 4 cut(s) 63, 82, 229, 420
Bst2UI CCWGG 3 cut(s) 192, 498, 730
Bst4CI ACNGT 3 cut(s) 154, 630, 867
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 840
BstAUI TGTACA 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 474, 607
BstDEI CTNAG 3 cut(s) 270, 480, 601
BstDSI CCRYGG 2 cut(s) 126, 402
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 4 cut(s) 97, 99, 115, 725
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 4 cut(s) 99, 584, 659, 758
BstKTI GATC 3 cut(s) 565, 701, 823
BstMAI GTCTC 2 cut(s) 476, 554
BstMBI GATC 3 cut(s) 562, 698, 820
BstMWI GCNNNNNNNGC 2 cut(s) 389, 882
BstNI CCWGG 3 cut(s) 192, 498, 730
BstNSI RCATGY 2 cut(s) 123, 351
BstSCI CCNGG 6 cut(s) 190, 281, 352, 353, 496, 728
BstSFI CTRYAG 2 cut(s) 35, 990
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 4 cut(s) 97, 99, 115, 725
BstV1I GCAGC 3 cut(s) 331, 400, 765
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuI GTATCC 1 cut(s) 801
BsuRI GGCC 7 cut(s) 81, 87, 261, 286, 392, 530, 728
BtgI CCRYGG 2 cut(s) 126, 402
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 474, 607
CfoI GCGC 4 cut(s) 99, 584, 659, 758
Cfr13I GGNCC 5 cut(s) 260, 284, 285, 391, 834
Cfr9I CCCGGG 1 cut(s) 353
CseI GACGC 2 cut(s) 731, 848
Csp6I GTAC 4 cut(s) 28, 117, 521, 622
CviAII CATG 3 cut(s) 120, 215, 348
CviQI GTAC 4 cut(s) 28, 117, 521, 622
DdeI CTNAG 3 cut(s) 270, 480, 601
DpnI GATC 3 cut(s) 564, 700, 822
DpnII GATC 3 cut(s) 562, 698, 820
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 726
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
EciI GGCGGA 1 cut(s) 965
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 4 cut(s) 63, 82, 229, 420
Eco24I GRGCYC 2 cut(s) 8, 288
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 601
Eco88I CYCGRG 2 cut(s) 353, 908
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 3 cut(s) 190, 496, 728
EcoT14I CCWWGG 4 cut(s) 63, 82, 229, 420
EcoT38I GRGCYC 2 cut(s) 8, 288
ErhI CCWWGG 4 cut(s) 63, 82, 229, 420
FaeI CATG 3 cut(s) 123, 218, 351
FaiI YATR 4 cut(s) 121, 216, 349, 633
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 2 cut(s) 108, 336
FatI CATG 3 cut(s) 119, 214, 347
Fnu4HI GCNGC 6 cut(s) 345, 390, 414, 726, 754, 979
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 2 cut(s) 8, 288
Fsp4HI GCNGC 6 cut(s) 345, 390, 414, 726, 754, 979
FspBI CTAG 1 cut(s) 575
GlaI GCGC 4 cut(s) 98, 583, 658, 757
GluI GCNGC 6 cut(s) 345, 390, 414, 726, 754, 979
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 7 cut(s) 81, 87, 261, 286, 392, 530, 728
HapII CCGG 3 cut(s) 282, 354, 648
HgaI GACGC 2 cut(s) 731, 848
HhaI GCGC 4 cut(s) 99, 584, 659, 758
Hin1I GRCGYC 1 cut(s) 840
Hin1II CATG 3 cut(s) 123, 218, 351
Hin6I GCGC 4 cut(s) 97, 582, 657, 756
HinP1I GCGC 4 cut(s) 97, 582, 657, 756
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 7 cut(s) 105, 134, 398, 426, 509, 765, 815
HpaII CCGG 3 cut(s) 282, 354, 648
HphI GGTGA 4 cut(s) 79, 631, 731, 980
Hpy166II GTNNAC 2 cut(s) 834, 997
Hpy188I TCNGA 4 cut(s) 310, 820, 825, 917
Hpy188III TCNNGA 2 cut(s) 254, 560
Hpy8I GTNNAC 2 cut(s) 834, 997
Hpy99I CGWCG 1 cut(s) 842
HpyAV CCTTC 2 cut(s) 189, 260
HpyCH4III ACNGT 3 cut(s) 154, 630, 867
HpyCH4V TGCA 6 cut(s) 76, 208, 326, 347, 371, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 882
HpyF3I CTNAG 3 cut(s) 270, 480, 601
Hsp92I GRCGYC 1 cut(s) 840
Hsp92II CATG 3 cut(s) 123, 218, 351
HspAI GCGC 4 cut(s) 97, 582, 657, 756
Kzo9I GATC 3 cut(s) 562, 698, 820
LmnI GCTCC 3 cut(s) 11, 193, 421
Lsp1109I GCAGC 3 cut(s) 331, 400, 765
LweI GCATC 3 cut(s) 195, 229, 313
MaeI CTAG 1 cut(s) 575
MaeIII GTNAC 4 cut(s) 18, 568, 719, 938
MalI GATC 3 cut(s) 564, 700, 822
MboI GATC 3 cut(s) 562, 698, 820
MboII GAAGA 5 cut(s) 462, 465, 607, 772, 805
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 2 cut(s) 8, 288
MluCI AATT 4 cut(s) 14, 490, 702, 898
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 4 cut(s) 82, 198, 798, 886
MnlI CCTC 6 cut(s) 19, 296, 302, 610, 875, 917
MseI TTAA 2 cut(s) 200, 299
MslI CAYNNNNRTG 2 cut(s) 65, 213
MspI CCGG 3 cut(s) 282, 354, 648
MspR9I CCNGG 6 cut(s) 192, 283, 354, 355, 498, 730
MvaI CCWGG 3 cut(s) 192, 498, 730
MvnI CGCG 4 cut(s) 97, 99, 115, 725
MwoI GCNNNNNNNGC 2 cut(s) 389, 882
NciI CCSGG 3 cut(s) 283, 354, 355
NdeII GATC 3 cut(s) 562, 698, 820
NlaIII CATG 3 cut(s) 123, 218, 351
NlaIV GGNNCC 2 cut(s) 286, 598
NmuCI GTSAC 2 cut(s) 568, 719
NspI RCATGY 2 cut(s) 123, 351
NspV TTCGAA 1 cut(s) 768
OliI CACNNNNGTG 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 908
PciI ACATGT 1 cut(s) 119
PfeI GAWTC 6 cut(s) 105, 134, 398, 426, 509, 765
PkrI GCNGC 6 cut(s) 346, 391, 415, 727, 755, 980
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PscI ACATGT 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 190, 496, 728
PspGI CCWGG 3 cut(s) 190, 496, 728
PspN4I GGNNCC 2 cut(s) 286, 598
PspOMI GGGCCC 1 cut(s) 284
PspPI GGNCC 5 cut(s) 260, 284, 285, 391, 834
PspXI VCTCGAGB 1 cut(s) 908
PstI CTGCAG 1 cut(s) 994
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 4 cut(s) 29, 118, 522, 623
RsaNI GTAC 4 cut(s) 28, 117, 521, 622
RseI CAYNNNNRTG 2 cut(s) 65, 213
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 200, 299
SatI GCNGC 6 cut(s) 345, 390, 414, 726, 754, 979
Sau3AI GATC 3 cut(s) 562, 698, 820
Sau96I GGNCC 5 cut(s) 260, 284, 285, 391, 834
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 6 cut(s) 192, 283, 354, 355, 498, 730
SduI GDGCHC 2 cut(s) 8, 288
SfaNI GCATC 3 cut(s) 195, 229, 313
SfcI CTRYAG 2 cut(s) 35, 990
Sfr274I CTCGAG 1 cut(s) 908
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 1 cut(s) 834
SlaI CTCGAG 1 cut(s) 908
SmaI CCCGGG 1 cut(s) 355
SmiMI CAYNNNNRTG 2 cut(s) 65, 213
SmlI CTYRAG 3 cut(s) 558, 812, 908
SmoI CTYRAG 3 cut(s) 558, 812, 908
Sse9I AATT 4 cut(s) 14, 490, 702, 898
SsiI CCGC 5 cut(s) 279, 389, 725, 976, 979
SspMI CTAG 1 cut(s) 575
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 6 cut(s) 190, 281, 352, 353, 496, 728
StyI CCWWGG 4 cut(s) 63, 82, 229, 420
TaaI ACNGT 3 cut(s) 154, 630, 867
TaqI TCGA 4 cut(s) 763, 768, 909, 923
TaqII GACCGA 1 cut(s) 851
TasI AATT 4 cut(s) 14, 490, 702, 898
TatI WGTACW 2 cut(s) 27, 520
TauI GCSGC 3 cut(s) 392, 728, 981
TfiI GAWTC 6 cut(s) 105, 134, 398, 426, 509, 765
Tru1I TTAA 2 cut(s) 200, 299
Tru9I TTAA 2 cut(s) 200, 299
TseFI GTSAC 2 cut(s) 568, 719
TseI GCWGC 3 cut(s) 344, 413, 753
Tsp45I GTSAC 2 cut(s) 568, 719
TspGWI ACGGA 2 cut(s) 172, 391
TspMI CCCGGG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 834
XapI RAATTY 1 cut(s) 898
XceI RCATGY 2 cut(s) 123, 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XhoI CTCGAG 1 cut(s) 908
XmaI CCCGGG 1 cut(s) 353
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.