MD08G1231000.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001187
Physical Location & Seq
Forward (+)
29639001 .. 29641456
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1231000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGAGCTCCTCCAAAATGTTATTCATTTACTTTCTACAGCTACTAGTTGGTTTGGCTACCACCTTGGTGATGGTGCAAGGCCAGGGCCAACTTACGCGCCTTGGATTCTATTCGCGTACATGTCCAGCAGCTGAGTCTATTGTCAAACAAACAGTTCAAACTCATTTCAACTCCAACCCTTCCGTTGCTCCTGGCTTGTTAAGGATGCACTTCCATGACTGCTTTGTCCAAGGTTGTGATGCTTCTGTCCTTCTTGACGGCCCTAATACTGAGAAAACCGCTGGGCCTAACGTCGGTTTAAGAGGTTGGGAAGTCATTGACGATGCAAAGACCAAGCTCGAAGCTGCATGCCCAGGAGTTGTTTCTTGTGCAGATATTCTCGCCCTCGCTGCCCGTGATTCCGTGGTTCTCGGAGAATGTGAACAATCAGCCGGAACTCGAAATCAAAACCAAAGGTTCACTTGGAATGTGCCTACTGGAAGAAAAGATGGACGAGTTTCGCTGGCCACCGAGACTAGTAATTTGCCTGGCTTTAGAGATTCCATTGATGTACAAAAGTGCAAGTTACTCAAGATCTTGTCACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

196

Amino Acids

21.13

Weight (kDa)

8.5

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 183 2.3e-44 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AccII CGCG 2 cut(s) 97, 115
AciI CCGC 1 cut(s) 279
AcoI YGGCCR 1 cut(s) 504
AfaI GTAC 2 cut(s) 118, 552
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 2 cut(s) 158, 169
AhlI ACTAGT 2 cut(s) 43, 515
AjnI CCWGG 4 cut(s) 81, 190, 352, 526
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 5 cut(s) 6, 40, 131, 337, 344
AluI AGCT 5 cut(s) 6, 40, 131, 337, 344
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 506
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 5 cut(s) 79, 85, 259, 284, 504
ApeKI GCWGC 3 cut(s) 128, 344, 389
ArsI GACNNNNNNTTYG 2 cut(s) 505, 537
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 85, 260, 284
AsuHPI GGTGA 1 cut(s) 79
BalI TGGCCA 1 cut(s) 506
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 3 cut(s) 140, 331, 376
BccI CCATC 2 cut(s) 64, 482
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 4 cut(s) 83, 192, 354, 528
BcoDI GTCTC 1 cut(s) 506
BcuI ACTAGT 2 cut(s) 43, 515
BfaI CTAG 2 cut(s) 44, 516
BfmI CTRYAG 1 cut(s) 35
BglII AGATCT 1 cut(s) 573
BisI GCNGC 3 cut(s) 129, 345, 390
BlsI GCNGC 3 cut(s) 130, 346, 391
Bme1390I CCNGG 4 cut(s) 83, 192, 354, 528
BmgT120I GGNCC 3 cut(s) 85, 260, 284
BmrFI CCNGG 4 cut(s) 83, 192, 354, 528
BmsI GCATC 3 cut(s) 195, 229, 313
BpuEI CTTGAG 1 cut(s) 554
BsaJI CCNNGG 6 cut(s) 63, 82, 100, 229, 352, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 481
BseBI CCWGG 4 cut(s) 83, 192, 354, 528
BseDI CCNNGG 6 cut(s) 63, 82, 100, 229, 352, 402
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 2 cut(s) 123, 261
BseNI ACTGG 1 cut(s) 481
BseXI GCAGC 3 cut(s) 140, 331, 376
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 2 cut(s) 97, 115
BshFI GGCC 5 cut(s) 81, 87, 261, 286, 506
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 506
BsnI GGCC 5 cut(s) 81, 87, 261, 286, 506
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 550
Bsp143I GATC 1 cut(s) 573
BspACI CCGC 1 cut(s) 279
BspANI GGCC 5 cut(s) 81, 87, 261, 286, 506
BspCNI CTCAG 2 cut(s) 124, 262
BspFNI CGCG 2 cut(s) 97, 115
BsrGI TGTACA 1 cut(s) 550
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 6 cut(s) 63, 82, 100, 229, 352, 402
BssMI GATC 1 cut(s) 573
BssT1I CCWWGG 3 cut(s) 63, 100, 229
Bst2UI CCWGG 4 cut(s) 83, 192, 354, 528
Bst4CI ACNGT 1 cut(s) 154
BstAUI TGTACA 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 349, 504
BstDEI CTNAG 2 cut(s) 132, 270
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 2 cut(s) 97, 115
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 1 cut(s) 576
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 1 cut(s) 573
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 4 cut(s) 83, 192, 354, 528
BstNSI RCATGY 2 cut(s) 123, 351
BstSCI CCNGG 4 cut(s) 81, 190, 352, 526
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 2 cut(s) 97, 115
BstV1I GCAGC 3 cut(s) 140, 331, 376
BstX2I RGATCY 1 cut(s) 573
BstYI RGATCY 1 cut(s) 573
BsuRI GGCC 5 cut(s) 81, 87, 261, 286, 506
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 349, 504
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 85, 260, 284
Csp6I GTAC 2 cut(s) 117, 551
CviAII CATG 3 cut(s) 120, 215, 348
CviQI GTAC 2 cut(s) 117, 551
DdeI CTNAG 2 cut(s) 132, 270
DpnI GATC 1 cut(s) 575
DpnII GATC 1 cut(s) 573
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 504
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 63, 100, 229
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 4 cut(s) 81, 190, 352, 526
EcoT14I CCWWGG 3 cut(s) 63, 100, 229
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 3 cut(s) 63, 100, 229
FaeI CATG 3 cut(s) 123, 218, 351
FaiI YATR 3 cut(s) 121, 216, 349
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FatI CATG 3 cut(s) 119, 214, 347
Fnu4HI GCNGC 3 cut(s) 129, 345, 390
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 129, 345, 390
FspBI CTAG 2 cut(s) 44, 516
GlaI GCGC 1 cut(s) 98
GluI GCNGC 3 cut(s) 129, 345, 390
GsaI CCCAGC 1 cut(s) 285
HaeIII GGCC 5 cut(s) 81, 87, 261, 286, 506
HapII CCGG 1 cut(s) 432
HhaI GCGC 1 cut(s) 99
Hin1II CATG 3 cut(s) 123, 218, 351
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 4 cut(s) 105, 134, 398, 539
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 422, 459
Hpy188I TCNGA 1 cut(s) 413
Hpy188III TCNNGA 2 cut(s) 254, 571
Hpy8I GTNNAC 2 cut(s) 422, 459
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 189, 260
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 291, 583
HpyCH4V TGCA 6 cut(s) 76, 208, 326, 347, 371, 561
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 2 cut(s) 132, 270
HpySE526I ACGT 2 cut(s) 291, 583
Hsp92II CATG 3 cut(s) 123, 218, 351
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 11, 193
Lsp1109I GCAGC 3 cut(s) 140, 331, 376
LweI GCATC 3 cut(s) 195, 229, 313
MaeI CTAG 2 cut(s) 44, 516
MaeII ACGT 2 cut(s) 291, 583
MaeIII GTNAC 2 cut(s) 564, 579
MalI GATC 1 cut(s) 575
MboI GATC 1 cut(s) 573
MboII GAAGA 1 cut(s) 492
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 1 cut(s) 520
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 3 cut(s) 19, 296, 395
Mox20I TGGCCA 1 cut(s) 506
MscI TGGCCA 1 cut(s) 506
MseI TTAA 2 cut(s) 200, 299
MslI CAYNNNNRTG 2 cut(s) 65, 213
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 2 cut(s) 131, 281
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 4 cut(s) 83, 192, 354, 528
MvaI CCWGG 4 cut(s) 83, 192, 354, 528
MvnI CGCG 2 cut(s) 97, 115
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 1 cut(s) 573
NlaIII CATG 3 cut(s) 123, 218, 351
NmuCI GTSAC 1 cut(s) 579
NspI RCATGY 2 cut(s) 123, 351
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 1 cut(s) 351
PciI ACATGT 1 cut(s) 119
PfeI GAWTC 3 cut(s) 105, 398, 539
PkrI GCNGC 3 cut(s) 130, 346, 391
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PscI ACATGT 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 4 cut(s) 81, 190, 352, 526
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 4 cut(s) 81, 190, 352, 526
PspPI GGNCC 3 cut(s) 85, 260, 284
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 118, 552
RsaNI GTAC 2 cut(s) 117, 551
RseI CAYNNNNRTG 2 cut(s) 65, 213
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 200, 299
SatI GCNGC 3 cut(s) 129, 345, 390
Sau3AI GATC 1 cut(s) 573
Sau96I GGNCC 3 cut(s) 85, 260, 284
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 4 cut(s) 83, 192, 354, 528
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 3 cut(s) 195, 229, 313
SfcI CTRYAG 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 65, 213
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SpeI ACTAGT 2 cut(s) 43, 515
SphI GCATGC 1 cut(s) 351
Sse9I AATT 1 cut(s) 520
SsiI CCGC 1 cut(s) 279
SspMI CTAG 2 cut(s) 44, 516
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 4 cut(s) 81, 190, 352, 526
StyI CCWWGG 3 cut(s) 63, 100, 229
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 294, 586
TaqI TCGA 2 cut(s) 339, 439
TasI AATT 1 cut(s) 520
TatI WGTACW 1 cut(s) 550
TfiI GAWTC 3 cut(s) 105, 398, 539
Tru1I TTAA 2 cut(s) 200, 299
Tru9I TTAA 2 cut(s) 200, 299
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 3 cut(s) 128, 344, 389
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 1 cut(s) 13
TspGWI ACGGA 2 cut(s) 172, 391
XceI RCATGY 2 cut(s) 123, 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XspI CTAG 2 cut(s) 44, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.