MD08G1230900.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001187
Physical Location & Seq
Forward (+)
29632630 .. 29633745
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1230900.v1.1.491

Sequence Viewer

Length: 996 bp
ATGAGCCCCTCCAAAATGTTCTTGATGTACTTTCTACAGGTACTTGTTCGTTTGGCTACCACCTTGGTGATGGTGCAAGGCCAGGGCCAACTTACACGAGTTGGATTCTACTCGCGTACATGTCCAGCAGCAGAGTCTATTGTCAAACAAACAGTTCAAACTCATTTCAACTCCAACCCTTCCGTTGCTCCTGGCTTGTTAAGGATGCACTTCCATGACTGCTTTGTCCAAGGTTGTGATGCTTCTGTCCTTCTTGACGGCCCTAATACTGAGAAAACCGCTGGGCCTAACCTCGGTTTAAGAGGTTGGGAAGTCATTGACGATGCAAAGACCAAGCTCGAAGCTGCATGCCCAGGAGTTGTTTCTTGTGCAGATATTCTCGCCCTCGCTGCCCGTGATTCCGTGGTTCTCACCAAAGGATTCACTTGGAATGTGCCTACCGGAAGAAAAGATGGACGAGTTTCGCTGGCCACCGAGACTAGTAATTTGCCTGGTTTTAGAGATTCGATTGATGTACAAAAGCGCAAGTTCCAAGATTTGGGTCTCAACACTCAAGATCTTGTCACGCTTGTTGGTGCACACACCATTGGCACCTCAGCTTGCCTGTTCTTCAGGTACAGACTGTACAACTTCACCACCACCGGAAATGGCGCTGATGAAACAATCAACCCTTTATTCATTGCCCAATTACGATCAATTTGCCCTGAAAATGGTGACACGGCCAGGCGTGTTGATTTAGACACGGGCAGCGCAAGTCGATTCGATGCAACTTTCTTCACAAATTTGAAGAATGGTCGCGGAATACTTGAGTCGGATCAGAAGTTATGGACCGACGTCACCACTAAGAGTTTTGTCCAACGATTTTTGGGCGTGAGAGGCTTACAAGCATTGAATTTCAACATCGAGTTCGGAAAGTCGATGGTGAAAATGAGTAACATCGGTGTAAAATCTGCCACAAATGGTGAAATCCGCCGCATTTGTTCTGCAGTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

332

Amino Acids

36.2

Weight (kDa)

9.03

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 48 - 288 5.7e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AatII GACGTC 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 590
AccB7I CCANNNNNTGG 1 cut(s) 538
AccII CGCG 2 cut(s) 115, 798
AciI CCGC 4 cut(s) 279, 798, 970, 973
AclWI GGATC 1 cut(s) 822
AcoI YGGCCR 2 cut(s) 468, 720
AcsI RAATTY 2 cut(s) 781, 892
AcuI CTGAAG 1 cut(s) 595
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 6 cut(s) 29, 42, 118, 516, 617, 626
AfiI CCNNNNNNNGG 3 cut(s) 293, 538, 710
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 5 cut(s) 158, 169, 787, 892, 898
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 5 cut(s) 81, 190, 352, 490, 722
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 3 cut(s) 337, 344, 599
AluI AGCT 3 cut(s) 337, 344, 599
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 2 cut(s) 470, 548
Alw44I GTGCAC 1 cut(s) 576
AlwI GGATC 1 cut(s) 822
AoxI GGCC 6 cut(s) 79, 85, 259, 284, 468, 720
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 4 cut(s) 128, 344, 389, 747
ApoI RAATTY 2 cut(s) 781, 892
ArsI GACNNNNNNTTYG 2 cut(s) 469, 501
AspLEI GCGC 3 cut(s) 525, 653, 752
AspS9I GGNCC 4 cut(s) 85, 260, 284, 828
AsuHPI GGTGA 7 cut(s) 79, 403, 625, 725, 829, 934, 974
AvaII GGWCC 1 cut(s) 828
BaeGI GKGCMC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 470
BanI GGYRCC 1 cut(s) 590
BanII GRGCYC 1 cut(s) 8
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 580
BbvCI CCTCAGC 1 cut(s) 595
BbvI GCAGC 4 cut(s) 140, 331, 376, 759
BccI CCATC 3 cut(s) 64, 446, 913
BceAI ACGGC 2 cut(s) 274, 735
BcgI CGANNNNNNTGC 2 cut(s) 681, 715
BciT130I CCWGG 5 cut(s) 83, 192, 354, 492, 724
BcoDI GTCTC 2 cut(s) 470, 548
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 2 cut(s) 35, 984
BfoI RGCGCY 1 cut(s) 654
BglII AGATCT 1 cut(s) 556
BisI GCNGC 5 cut(s) 129, 345, 390, 748, 973
BlsI GCNGC 5 cut(s) 130, 346, 391, 749, 974
Bme1390I CCNGG 5 cut(s) 83, 192, 354, 492, 724
Bme18I GGWCC 1 cut(s) 828
BmgT120I GGNCC 4 cut(s) 85, 260, 284, 828
BmiI GGNNCC 1 cut(s) 592
BmrFI CCNGG 5 cut(s) 83, 192, 354, 492, 724
BmsI GCATC 4 cut(s) 195, 229, 313, 754
BoxI GACNNNNGTC 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 537, 827
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 6 cut(s) 63, 82, 229, 292, 352, 402
BsaWI WCCGGW 2 cut(s) 440, 641
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 538, 710
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 5 cut(s) 83, 192, 354, 492, 724
BseDI CCNNGG 6 cut(s) 63, 82, 229, 292, 352, 402
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 3 cut(s) 293, 538, 710
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 261, 609
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 4 cut(s) 140, 331, 376, 759
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 2 cut(s) 115, 798
BshFI GGCC 6 cut(s) 81, 87, 261, 286, 470, 722
BshNI GGYRCC 1 cut(s) 590
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 2 cut(s) 441, 642
BslI CCNNNNNNNGG 3 cut(s) 293, 538, 710
BsmAI GTCTC 2 cut(s) 470, 548
BsnI GGCC 6 cut(s) 81, 87, 261, 286, 470, 722
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 8, 580
Bsp1407I TGTACA 2 cut(s) 514, 624
Bsp143I GATC 3 cut(s) 556, 692, 814
BspACI CCGC 4 cut(s) 279, 798, 970, 973
BspANI GGCC 6 cut(s) 81, 87, 261, 286, 470, 722
BspCNI CTCAG 2 cut(s) 262, 608
BspFNI CGCG 2 cut(s) 115, 798
BspLI GGNNCC 1 cut(s) 592
BspMAI CTGCAG 1 cut(s) 988
BspPI GGATC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 678
BsrGI TGTACA 2 cut(s) 514, 624
BssECI CCNNGG 6 cut(s) 63, 82, 229, 292, 352, 402
BssMI GATC 3 cut(s) 556, 692, 814
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 63, 229
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 5 cut(s) 83, 192, 354, 492, 724
Bst4CI ACNGT 2 cut(s) 154, 624
BstACI GRCGYC 1 cut(s) 834
BstAUI TGTACA 2 cut(s) 514, 624
BstC8I GCNNGC 3 cut(s) 349, 468, 601
BstDEI CTNAG 3 cut(s) 270, 595, 843
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 2 cut(s) 115, 798
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 3 cut(s) 525, 653, 752
BstKTI GATC 3 cut(s) 559, 695, 817
BstMAI GTCTC 2 cut(s) 470, 548
BstMBI GATC 3 cut(s) 556, 692, 814
BstMWI GCNNNNNNNGC 2 cut(s) 389, 876
BstNI CCWGG 5 cut(s) 83, 192, 354, 492, 724
BstNSI RCATGY 2 cut(s) 123, 351
BstPAI GACNNNNGTC 1 cut(s) 833
BstSCI CCNGG 5 cut(s) 81, 190, 352, 490, 722
BstSFI CTRYAG 2 cut(s) 35, 984
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 2 cut(s) 115, 798
BstV1I GCAGC 4 cut(s) 140, 331, 376, 759
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BsuRI GGCC 6 cut(s) 81, 87, 261, 286, 470, 722
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 349, 468, 601
CfoI GCGC 3 cut(s) 525, 653, 752
Cfr13I GGNCC 4 cut(s) 85, 260, 284, 828
Csp6I GTAC 6 cut(s) 28, 41, 117, 515, 616, 625
CviAII CATG 3 cut(s) 120, 215, 348
CviQI GTAC 6 cut(s) 28, 41, 117, 515, 616, 625
DdeI CTNAG 3 cut(s) 270, 595, 843
DpnI GATC 3 cut(s) 558, 694, 816
DpnII GATC 3 cut(s) 556, 692, 814
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 2 cut(s) 468, 720
EciI GGCGGA 1 cut(s) 959
Eco130I CCWWGG 2 cut(s) 63, 229
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 828
Eco57I CTGAAG 1 cut(s) 595
EcoRII CCWGG 5 cut(s) 81, 190, 352, 490, 722
EcoT14I CCWWGG 2 cut(s) 63, 229
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 2 cut(s) 63, 229
FaeI CATG 3 cut(s) 123, 218, 351
FaiI YATR 4 cut(s) 121, 216, 349, 826
FatI CATG 3 cut(s) 119, 214, 347
Fnu4HI GCNGC 5 cut(s) 129, 345, 390, 748, 973
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 129, 345, 390, 748, 973
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 524, 652, 751
GluI GCNGC 5 cut(s) 129, 345, 390, 748, 973
GsaI CCCAGC 1 cut(s) 285
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 6 cut(s) 81, 87, 261, 286, 470, 722
HapII CCGG 2 cut(s) 441, 642
HhaI GCGC 3 cut(s) 525, 653, 752
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 3 cut(s) 123, 218, 351
Hin6I GCGC 3 cut(s) 523, 651, 750
HinP1I GCGC 3 cut(s) 523, 651, 750
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 7 cut(s) 105, 134, 398, 420, 503, 759, 809
HpaII CCGG 2 cut(s) 441, 642
HphI GGTGA 7 cut(s) 79, 403, 625, 725, 829, 934, 974
Hpy166II GTNNAC 2 cut(s) 578, 991
Hpy188I TCNGA 3 cut(s) 814, 819, 911
Hpy188III TCNNGA 3 cut(s) 22, 254, 554
Hpy8I GTNNAC 2 cut(s) 578, 991
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 2 cut(s) 189, 260
HpyCH4III ACNGT 2 cut(s) 154, 624
HpyCH4IV ACGT 1 cut(s) 834
HpyCH4V TGCA 8 cut(s) 76, 208, 326, 347, 371, 578, 767, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 876
HpyF3I CTNAG 3 cut(s) 270, 595, 843
HpySE526I ACGT 1 cut(s) 834
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 3 cut(s) 123, 218, 351
HspAI GCGC 3 cut(s) 523, 651, 750
Kzo9I GATC 3 cut(s) 556, 692, 814
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 4 cut(s) 140, 331, 376, 759
LweI GCATC 4 cut(s) 195, 229, 313, 754
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 4 cut(s) 562, 713, 835, 932
MalI GATC 3 cut(s) 558, 694, 816
MboI GATC 3 cut(s) 556, 692, 814
MboII GAAGA 4 cut(s) 456, 601, 766, 799
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 2 cut(s) 8, 580
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 5 cut(s) 484, 686, 696, 781, 892
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 143, 818
MmeI TCCRAC 4 cut(s) 82, 198, 792, 880
MnlI CCTC 6 cut(s) 19, 296, 302, 395, 604, 869
Mox20I TGGCCA 1 cut(s) 470
MscI TGGCCA 1 cut(s) 470
MseI TTAA 2 cut(s) 200, 299
MslI CAYNNNNRTG 2 cut(s) 65, 213
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 441, 642
MspR9I CCNGG 5 cut(s) 83, 192, 354, 492, 724
MvaI CCWGG 5 cut(s) 83, 192, 354, 492, 724
MvnI CGCG 2 cut(s) 115, 798
MwoI GCNNNNNNNGC 2 cut(s) 389, 876
NdeII GATC 3 cut(s) 556, 692, 814
NlaIII CATG 3 cut(s) 123, 218, 351
NlaIV GGNNCC 1 cut(s) 592
NmuCI GTSAC 3 cut(s) 562, 713, 835
NspI RCATGY 2 cut(s) 123, 351
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 1 cut(s) 351
PciI ACATGT 1 cut(s) 119
PfeI GAWTC 5 cut(s) 105, 398, 420, 503, 759
PflMI CCANNNNNTGG 1 cut(s) 538
PkrI GCNGC 5 cut(s) 130, 346, 391, 749, 974
PleI GAGTC 2 cut(s) 142, 817
PpsI GAGTC 2 cut(s) 142, 817
PscI ACATGT 1 cut(s) 119
PshAI GACNNNNGTC 1 cut(s) 833
Psp6I CCWGG 5 cut(s) 81, 190, 352, 490, 722
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 5 cut(s) 81, 190, 352, 490, 722
PspN4I GGNNCC 1 cut(s) 592
PspPI GGNCC 4 cut(s) 85, 260, 284, 828
PstI CTGCAG 1 cut(s) 988
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 6 cut(s) 29, 42, 118, 516, 617, 626
RsaNI GTAC 6 cut(s) 28, 41, 117, 515, 616, 625
RseI CAYNNNNRTG 2 cut(s) 65, 213
SaqAI TTAA 2 cut(s) 200, 299
SatI GCNGC 5 cut(s) 129, 345, 390, 748, 973
Sau3AI GATC 3 cut(s) 556, 692, 814
Sau96I GGNCC 4 cut(s) 85, 260, 284, 828
SchI GAGTC 2 cut(s) 143, 818
ScrFI CCNGG 5 cut(s) 83, 192, 354, 492, 724
SduI GDGCHC 2 cut(s) 8, 580
SfaNI GCATC 4 cut(s) 195, 229, 313, 754
SfcI CTRYAG 2 cut(s) 35, 984
SinI GGWCC 1 cut(s) 828
SmiMI CAYNNNNRTG 2 cut(s) 65, 213
SmlI CTYRAG 2 cut(s) 552, 806
SmoI CTYRAG 2 cut(s) 552, 806
SpeI ACTAGT 1 cut(s) 479
SphI GCATGC 1 cut(s) 351
Sse9I AATT 5 cut(s) 484, 686, 696, 781, 892
SsiI CCGC 4 cut(s) 279, 798, 970, 973
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 5 cut(s) 81, 190, 352, 490, 722
StyI CCWWGG 2 cut(s) 63, 229
TaaI ACNGT 2 cut(s) 154, 624
TaiI ACGT 1 cut(s) 837
TaqI TCGA 6 cut(s) 339, 506, 757, 762, 903, 917
TaqII GACCGA 1 cut(s) 845
TasI AATT 5 cut(s) 484, 686, 696, 781, 892
TatI WGTACW 3 cut(s) 27, 514, 624
TauI GCSGC 1 cut(s) 975
TfiI GAWTC 5 cut(s) 105, 398, 420, 503, 759
Tru1I TTAA 2 cut(s) 200, 299
Tru9I TTAA 2 cut(s) 200, 299
TseFI GTSAC 3 cut(s) 562, 713, 835
TseI GCWGC 4 cut(s) 128, 344, 389, 747
Tsp45I GTSAC 3 cut(s) 562, 713, 835
TspDTI ATGAA 2 cut(s) 667, 672
TspGWI ACGGA 2 cut(s) 172, 391
Van91I CCANNNNNTGG 1 cut(s) 538
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 1 cut(s) 828
XapI RAATTY 2 cut(s) 781, 892
XceI RCATGY 2 cut(s) 123, 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XspI CTAG 1 cut(s) 480
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.