Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001928

Physical Location & Seq

Genomic Coordinates Reverse (-)

37567558 .. 37576985

Gene Structure 9428 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1293000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4164 bp

ATGGCTATGGCAGTGGAGAAGAAGAGCAGTTCATGGCGTCTGCAGAGCGGCCAATACCTCGGAGAAATCTCAGCCCTTTGCTTCCTCCACCTTCCTTCTCCCCTCTCTGCTCTCCCTTACCTACTCGCAGGGTCGGGTTCGCAAATCATGGTGTATGATTTGGAAATGGGAAGAATGGTGAGATCGTTCGACGTGTTCCAAGGGATTCGAGTGCACGGGATAATCTGTTATAGCTCTGGTTCTAGTACTGAACGCGCAGAGGGAACACTGGCGTCTTCTATTGCTTTCAAAATTTCCGTGTTCGGCGAAAGAAGAGTGAAGTTGTTTAGTTTGCAGATTGAAACGGTGCAGCAAGTCAATGTCAGTTTGACTCTATTGCAATCCTTGCCAAAGTTCTCAAATTGGGTCTTGGATGTTTCCTTCTTTAAGGGCTCTGTGAGTGGCTCATATGGGGAGGGTGATTGTCTTGCTATAGGGTGTAGTGACAACTCTGTCCATCTATGGGATGTCTCAGCTTCTGCTGTGGTTCTTGAAGTTCGACATCCTGAGAGGACCCTTCTTTATTCAATGCGGTTATGGGGAGACAATCTTCAAGCTCTCCGTGTTGCATCTGGTACCATCTATAATGAGATCATTGTATGGAAAGTGGTGTCTCAGTATAATGCTGCATCTTTAACAAATCAAGTAGAAGACGGTATTGATCAGATAAATTCGTTCTCAGACTCTGTTCAGCCTCATGCTTGCCAGTATGAAGCTGTTCATGTAAGCAAACTTGTTGGACATGAAGGTTCCATCTTTCGGATAGCCTGGTCCTTTAATGGAACCAAATTGGTTTCTGTCTCTGACGATCGTAGTGCTCGTGTCTGGGCAGCTTGTGCGGAAACAAAGCATTCTGAGAATCCAGGAGAGTCCATTGGCCTTGTGCTGTTTGGTCACAATGCCCGAGTTTGGGACTGCTGTATTCTTGGTTCGTTGATTGTCACTGCTGGCGAGGATTGCACATGTCGTGTGTGGGGACTGGATGGTAAACACCTTCAGATGATCAAGGAGCACACAGGAAGGGGCATATGGCGTTGTTTGTATGATCCAAACTCTTCGCTTCTCATTACTGCTGGTTTTGATTCTGCAATTAAAGTGCATCAGCTGCACACATCCTCTGGGAGCCTGGAGGGACATGCAGATACAAAAGAATTTAATAGAACGATTGCATACACAGTTCATATCCCTACTTTTTCAGAGAATATTGGACCTATGGACAGCAAAAGCGAATACGTCCGTTGCTTGAGCTTTGCACGTGAGGATACCCTTTATGTTTCAACAAACCACGGCTATCTGTACCATGCTAGATTATTGAACACTGGAGAAGTTGAATGGACTGAACTCGTCCGCCTCAGTCAGGAGGTCCAGATTGTTTGTATGGATTTGTTGTCAGAACCACTTGAATTTTGCTCCAGCATTGAGGACTGGGTTTCTGTTGGAGACGGAAAAGGAAACGTTACAATAGTTGGGGTTATACGTGATGCTTGTACTCCTAAAGTGGGTTTCTCCCTTACTTGGTCAGCTGGGATAGAGAGGCAACTCCTAGGAACACATTGGTCCAAGTCACTAGGTTCTGGGTATATCTTCACCGCCGATCCTAGAGGAACATTAAAGCTCTGGAGGTTGTCTGACCATTCTGCAAGGAGCTGCAATGTCTTTCTTATGGCAGAGTTCACATCAATTTTTGGAACTCGAATAATGTGTTTGGATGCATTGTTGGAGGAAGAGGTATTGGTATGTGGAGATATACGTGGTAATCTTGTTTTGTTCCCTCTGTTGAAGAGTGTCTTGCATGGAACGGTAGAACCTAACATGAAAATATCTCCGTCCAATTACTTCAAAGGAGCTCATGGAATATCAAGTGTTTCAAGTTTGGCTGTTGCTAGACTAAGCTCCGAAAAGATTGAAATACGCTCGACAGGAGCAGATGGGTGTATATGCTATCTGGAGTATGAAAGAGATATGAAGAGTTTGCAATTTATAGGGATGAAACAAGTGAAAGAGTTGAGTCTTATTGAATCTGTTTCTGATAATAGCTCTGTTAATGAGTTGTCAACCTGTCATTGTGCTGCTGGTTTTGCATCAGTGGATTTTATAATTTGGAATTTAATAACTGAAACCAAGGTTGTCCAAATTCCATGTGGTGGATGGAGGCGGCCGCATTCCTATTATCTTGGTGATATACCAGAAATAAAGAACTGTTTTGCATATGTTAAGGATGAGATCATTTATATCCATAGGCGATGGGTGCTTGATGGTGAAAGGAAGATACTCTCCAGGAATTTGCATATGCAATTTCATGGGAGAGAGATGCATTCCATATGCTTTGTCTCTGAAGATTTGCAACCTGGAGTAGATGAGAAGTACAGCCTATTCACTGGATCTAGTTGGATGGCTACTGGGTGTGAAGATGGAACTGTGAGGTTGACTAGGTATACGCCAGGTGTTGAAAATTGGTCTGCATCAAAATTGCTTGGGGAGCATGTTGGTGGATCAGCTGTGAGATCAATATGTTGTGTGTCAAAGTTATACATTGTTCCATCAGATGCGGCCAGCATAAATGAAAAAAATGCAGCAGCAGAGAACAGAGAAACTCCAGTTTTATTGATTTCAGTTGGTGCAAAGCGGGTCTTAACTTCTTGGCTACTAAGAAGTAATAAGGTGAACAAAAAGGATGAAATACTATCTGATCAGCAGCATAACATCACTGGAAATGGAAATAAAGTTTTGTCGCAAGAGTCATCTTCAATGTCATTCCAGTGGCTTTCAACAGATATGCCAGTCAAGTATTCTAGTAATCCAAAATTTCCAGACAATATAGGGAAAAAAATTGGTCTAGCAGGGAATGTTTCTACTGAAGATGCTGATGCAGGATCTAGATTAGTGTCTTCAAAAAAGGGAAATATGGAGTTCAAATCTTCAGTTAAGGATAAATATGAAGATGACTGGAGATACCTGGCTGTCACTGCGTTTTTGGTCAAATGTGCTGGTTCCAGGATTACCGTTTGTTTTATTGTTGTTGCTTGTTCAGATGCCACCCTTGCATTGCGGGCTCTAGTTTTGCCCTATAGGCTATGGTTTGATGTTTCTGTGTTGGTTCCTCTATCATCACCAGTTTTGGCATTGCAGCATGTCATCCTTCCCACATGCGTCCCTTCTGAAGATAATGTTCAGAGGGGGTGTTTGTATATTCTCATTAGTGGGGCTACTGACGGAAGTATTGCCTTTTGGGACCTCACCAGAAGCATTCAAGCTTTCATGCAACTTGTATCAGTACTTGATGTTGCAAAGTTCATTGATTGTCAGAAAAGGCCACGGACAGGGAGGGGAAGTCAGGGTGGACGACAGTGGAGATCTTTAGGCAGTAGCATGTCAAAGAATAGACTTGGCACTGGTTCCACCACTGTAAAAGCTGGAGAGGAAACTAAATGCAATTTGCCTAATCACATTGTGGATGGAATGACATCGATGCAAAATGATCACGAGAGCAGAACAGTTTCTTCCCAAGCTGACGATACTGCTTCCGAAGTGAATGCTTCTGATTCCTCATCTGATATATGTGAAATATCTCCTTTAGTTGTTTTCAAGACCATTCATCAATCGGGTGTCAATTCTCTCCATGTTTCAGATGTAGAGGGTTGTCGAAGTCCTGAAATTGGGTTTCTGTACAATTTAATTAGTGGCGGTGATGATCAAGCGCTTAGTTGTCTTACGTTTGAATTGTCAGTTTCAGCATCAAACACTGGATCTGAGAATATGGCACTGGAAATTACAAATTCAGTCACTGAACTTGGAAGTACAAAAAAAATTATTCACTCTAGTCAAGACAAGAACTATTGGATCAGATTCCTAAATCATGATAAAGTCCCTTCAGCTCACAGCTCTGCTGTAAAAGGTGTTTGGACAGATGGATCTTGGGTTTTTTCCACTGGTCTTGATCAACGTGTCAGGTGCTGGCGTCTAGAGGATGAAGGTAAACTAATCAAGGGTTCTTATTTCATCATAAGTGTGCCAGAGCCAGAAGCGCTGGATGCTAAAGTGTGTGGCCGGAACCATTATCAGATTGCAGTAGCTGGGAGAGGAATGCAAATGCTTGAGTTCTCTGAATCTTTAGATGTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0007049 GO:0007050 GO:0008150 GO:0008285 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010506 GO:0010507 GO:0016020 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0042127 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1388

Amino Acids

152.68

Weight (kDa)

6.01

Isoelectric Point (pI)

37.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	255 - 289	6.8e-06	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	256 - 381	2e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	258 - 385	1.5e-11	WDR5 beta-propeller domain
EIF3I	PF24805	258 - 368	7.1e-06	EIF3I
Beta-prop_THOC3	PF25174	261 - 387	2.8e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001928)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01860 AT4G01860
fragaria_vesca	FvH4_1g26470 FvH4_2g08080
malus_domestica	MD09G1293000.v1.1 MD17G1281300.v1.1 MD17G1281400.v1.1 MD17G1281500.v1.1 MD17G1281700.v1.1 MD17G1281800.v1.1
prunus_persica	Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1
pyrus_communis	pycom09g19610 pycom09g19620 pycom09g19630 pycom17g27900
rosa_chinensis	RchiOBHm_Chr3g0486381 RchiOBHm_Chr3g0486491 RchiOBHm_Chr3g0486501 RchiOBHm_Chr3g0486511
rosa_laevigata	RLG00000023079 RLG00000023084 RLG00000023086 RLG00000023087
rosa_multiflora	Rmu_sc0001857.1_g000004 Rmu_sc0003801.1_g000001 Rmu_sc0003801.1_g000026 Rmu_sc0011408.1_g000006
rosa_roxburghii	Rroxscaffold_1G00022380 Rroxscaffold_3G00237910 Rroxscaffold_3G00237930 Rroxscaffold_3G00237990 Rroxscaffold_6G00395820
rosa_rugosa	Rorug03G0224900
rosa_samantha	Rh3AG274900 Rh3BG310000 Rh3CG309200 Rh3DG305300 Rh3DG306000
rosa_wichuraiana	Rw3G024390 Rw3G024500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2135
AasI	GACNNNNNNGTC	1 cut(s)	491
Acc65I	GGTACC	1 cut(s)	614
AccB1I	GGYRCC	1 cut(s)	614
AccB7I	CCANNNNNTGG	1 cut(s)	2183
AccBSI	CCGCTC	1 cut(s)	48
AccI	GTMKAC	1 cut(s)	2474
AccII	CGCG	1 cut(s)	255
AclI	AACGTT	1 cut(s)	1494
AclWI	GGATC	8 cut(s)	1079, 1628, 2428, 2539, 2920, 3793, 3887, 3958
AcoI	YGGCCR	4 cut(s)	49, 2195, 2589, 4084
AcsI	RAATTY	9 cut(s)	291, 709, 1190, 1442, 2143, 2172, 2320, 2843, 3814
AcuI	CTGAAG	6 cut(s)	1019, 2394, 2916, 2943, 3219, 3894
AcvI	CACGTG	1 cut(s)	1295
AcyI	GRCGYC	3 cut(s)	37, 272, 3997
AdeI	CACNNNGTG	1 cut(s)	3490
AfaI	GTAC	8 cut(s)	247, 616, 1337, 1528, 2405, 3315, 3707, 3838
AfeI	AGCGCT	2 cut(s)	3738, 4065
AflIII	ACRYGT	3 cut(s)	192, 1001, 3982
AjiI	CACGTC	1 cut(s)	193
AjnI	CCWGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
Alw21I	GWGCWC	4 cut(s)	216, 859, 1053, 1888
Alw26I	GTCTC	6 cut(s)	514, 576, 657, 844, 1473, 2374
Alw44I	GTGCAC	1 cut(s)	212
AlwI	GGATC	8 cut(s)	1079, 1628, 2428, 2539, 2920, 3793, 3887, 3958
AlwNI	CAGNNNCTG	5 cut(s)	518, 725, 3824, 3993, 4112
Ama87I	CYCGRG	1 cut(s)	942
Aor51HI	AGCGCT	2 cut(s)	3738, 4065
AoxI	GGCC	6 cut(s)	49, 916, 2195, 2589, 3350, 4084
ApaLI	GTGCAC	1 cut(s)	212
ApoI	RAATTY	9 cut(s)	291, 709, 1190, 1442, 2143, 2172, 2320, 2843, 3814
Asp700I	GAANNNNTTC	2 cut(s)	756, 3535
Asp718I	GGTACC	1 cut(s)	614
AspA2I	CCTAGG	1 cut(s)	1582
AspLEI	GCGC	3 cut(s)	257, 3739, 4066
AspS9I	GGNCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
AsuHPI	GGTGA	9 cut(s)	190, 470, 1618, 2228, 2309, 2713, 3141, 3268, 3737
AvaI	CYCGRG	1 cut(s)	942
AvaII	GGWCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
AvrII	CCTAGG	1 cut(s)	1582
BaeGI	GKGCMC	1 cut(s)	216
BanI	GGYRCC	1 cut(s)	614
BanII	GRGCYC	3 cut(s)	434, 1888, 3094
BauI	CACGAG	2 cut(s)	858, 3521
BbrPI	CACGTG	1 cut(s)	1295
BbsI	GAAGAC	3 cut(s)	267, 696, 2919
Bbv12I	GWGCWC	4 cut(s)	216, 859, 1053, 1888
BceAI	ACGGC	1 cut(s)	1342
BcgI	CGANNNNNNTGC	4 cut(s)	836, 870, 3554, 3588
BciT130I	CCWGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
BciVI	GTATCC	1 cut(s)	1294
BclI	TGATCA	6 cut(s)	700, 1041, 2728, 3517, 3730, 3976
BcoDI	GTCTC	6 cut(s)	514, 576, 657, 844, 1473, 2374
BfmI	CTRYAG	3 cut(s)	41, 471, 3106
BfoI	RGCGCY	2 cut(s)	3740, 4067
BfuI	GTATCC	1 cut(s)	1294
BglI	GCCNNNNNGGC	1 cut(s)	3109
BglII	AGATCT	1 cut(s)	3392
BlnI	CCTAGG	1 cut(s)	1582
BmcAI	AGTACT	2 cut(s)	247, 3315
Bme1390I	CCNGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
Bme18I	GGWCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
BmeT110I	CYCGRG	1 cut(s)	942
BmgBI	CACGTC	1 cut(s)	193
BmgT120I	GGNCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
BmrFI	CCNGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
BmrI	ACTGGG	2 cut(s)	1474, 2448
BmuI	ACTGGG	2 cut(s)	1474, 2448
BpiI	GAAGAC	3 cut(s)	267, 696, 2919
BpuEI	CTTGAG	2 cut(s)	1303, 4154
Bsa29I	ATCGAT	1 cut(s)	3506
BsaAI	YACGTR	3 cut(s)	1295, 1517, 1790
BsaHI	GRCGYC	3 cut(s)	37, 272, 3997
BsaJI	CCNNGG	6 cut(s)	58, 199, 1324, 1582, 2160, 3353
BsaXI	ACNNNNNCTCC	2 cut(s)	3355, 3385
Bse3DI	GCAATG	3 cut(s)	1696, 3083, 3161
BseBI	CCWGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
BseCI	ATCGAT	1 cut(s)	3506
BseDI	CCNNGG	6 cut(s)	58, 199, 1324, 1582, 2160, 3353
BseMI	GCAATG	3 cut(s)	1696, 3083, 3161
BseMII	CTCAG	8 cut(s)	84, 525, 537, 668, 732, 885, 1405, 3780
BseSI	GKGCMC	1 cut(s)	216
BseX3I	CGGCCG	1 cut(s)	2195
BseYI	CCCAGC	2 cut(s)	1562, 4112
BsgI	GTGCAG	2 cut(s)	368, 1130
Bsh1236I	CGCG	1 cut(s)	255
Bsh1285I	CGRYCG	2 cut(s)	850, 2198
BshFI	GGCC	6 cut(s)	51, 918, 2197, 2591, 3352, 4086
BshNI	GGYRCC	1 cut(s)	614
BshVI	ATCGAT	1 cut(s)	3506
BsiEI	CGRYCG	2 cut(s)	850, 2198
BsiHKAI	GWGCWC	4 cut(s)	216, 859, 1053, 1888
BsiHKCI	CYCGRG	1 cut(s)	942
BsiSI	CCGG	1 cut(s)	4087
BslFI	GGGAC	6 cut(s)	965, 1029, 1185, 3176, 3284, 3890
BsmAI	GTCTC	6 cut(s)	514, 576, 657, 844, 1473, 2374
BsmBI	CGTCTC	1 cut(s)	1473
BsmFI	GGGAC	6 cut(s)	965, 1029, 1185, 3176, 3284, 3890
BsmI	GAATGC	6 cut(s)	889, 2200, 2353, 3285, 3577, 4128
BsnI	GGCC	6 cut(s)	51, 918, 2197, 2591, 3352, 4086
BsoBI	CYCGRG	1 cut(s)	942
Bsp1286I	GDGCHC	6 cut(s)	216, 434, 859, 1053, 1888, 3094
Bsp1407I	TGTACA	1 cut(s)	3705
BspANI	GGCC	6 cut(s)	51, 918, 2197, 2591, 3352, 4086
BspCNI	CTCAG	8 cut(s)	83, 524, 538, 667, 731, 886, 1404, 3781
BspDI	ATCGAT	1 cut(s)	3506
BspFNI	CGCG	1 cut(s)	255
BspHI	TCATGA	1 cut(s)	3895
BspMAI	CTGCAG	1 cut(s)	45
BspPI	GGATC	8 cut(s)	1079, 1628, 2428, 2539, 2920, 3793, 3887, 3958
BspQI	GCTCTTC	1 cut(s)	17
BspT107I	GGYRCC	1 cut(s)	614
BsrBI	CCGCTC	1 cut(s)	48
BsrDI	GCAATG	3 cut(s)	1696, 3083, 3161
BsrGI	TGTACA	1 cut(s)	3705
BssECI	CCNNGG	6 cut(s)	58, 199, 1324, 1582, 2160, 3353
BssNAI	GTATAC	1 cut(s)	2475
BssNI	GRCGYC	3 cut(s)	37, 272, 3997
BssSI	CACGAG	2 cut(s)	858, 3521
BssT1I	CCWWGG	3 cut(s)	199, 1582, 2160
Bst1107I	GTATAC	1 cut(s)	2475
Bst2BI	CACGAG	2 cut(s)	858, 3521
Bst2UI	CCWGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
Bst6I	CTCTTC	6 cut(s)	17, 307, 1099, 1758, 1814, 2000
BstACI	GRCGYC	3 cut(s)	37, 272, 3997
BstAPI	GCANNNNNTGC	3 cut(s)	385, 875, 1144
BstAUI	TGTACA	1 cut(s)	3705
BstBAI	YACGTR	3 cut(s)	1295, 1517, 1790
BstC8I	GCNNGC	5 cut(s)	742, 988, 2593, 3090, 3995
BstDSI	CCRYGG	2 cut(s)	1324, 3353
BstENI	CCTNNNNNAGG	1 cut(s)	1394
BstFNI	CGCG	1 cut(s)	255
BstH2I	RGCGCY	2 cut(s)	3740, 4067
BstHHI	GCGC	3 cut(s)	257, 3739, 4066
BstMAI	GTCTC	6 cut(s)	514, 576, 657, 844, 1473, 2374
BstMCI	CGRYCG	2 cut(s)	850, 2198
BstNI	CCWGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
BstNSI	RCATGY	6 cut(s)	1005, 1178, 2525, 3173, 3189, 3412
BstSCI	CCNGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
BstSFI	CTRYAG	3 cut(s)	41, 471, 3106
BstSLI	GKGCMC	1 cut(s)	216
BstUI	CGCG	1 cut(s)	255
BstV2I	GAAGAC	3 cut(s)	267, 696, 2919
BstX2I	RGATCY	5 cut(s)	2420, 2912, 3392, 3785, 3950
BstYI	RGATCY	5 cut(s)	2420, 2912, 3392, 3785, 3950
BstZ17I	GTATAC	1 cut(s)	2475
BstZI	CGGCCG	1 cut(s)	2195
Bsu15I	ATCGAT	1 cut(s)	3506
BsuI	GTATCC	1 cut(s)	1294
BsuRI	GGCC	6 cut(s)	51, 918, 2197, 2591, 3352, 4086
BsuTUI	ATCGAT	1 cut(s)	3506
BtgI	CCRYGG	2 cut(s)	1324, 3353
BtgZI	GCGATG	1 cut(s)	2296
BtrI	CACGTC	1 cut(s)	193
BtsI	GCAGTG	3 cut(s)	18, 981, 3003
Cac8I	GCNNGC	5 cut(s)	742, 988, 2593, 3090, 3995
CaiI	CAGNNNCTG	5 cut(s)	518, 725, 3824, 3993, 4112
CciI	TCATGA	1 cut(s)	3895
CciNI	GCGGCCGC	1 cut(s)	2195
CfoI	GCGC	3 cut(s)	257, 3739, 4066
Cfr13I	GGNCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
ClaI	ATCGAT	1 cut(s)	3506
CseI	GACGC	4 cut(s)	26, 261, 3178, 3986
Csp6I	GTAC	8 cut(s)	246, 615, 1336, 1527, 2404, 3314, 3706, 3837
CspCI	CAANNNNNGTGG	2 cut(s)	3955, 3990
CviQI	GTAC	8 cut(s)	246, 615, 1336, 1527, 2404, 3314, 3706, 3837
DraIII	CACNNNGTG	1 cut(s)	3490
DrdI	GACNNNNNNGTC	1 cut(s)	491
DseDI	GACNNNNNNGTC	1 cut(s)	491
EaeI	YGGCCR	4 cut(s)	49, 2195, 2589, 4084
EagI	CGGCCG	1 cut(s)	2195
Eam1104I	CTCTTC	6 cut(s)	17, 307, 1099, 1758, 1814, 2000
EarI	CTCTTC	6 cut(s)	17, 307, 1099, 1758, 1814, 2000
EciI	GGCGGA	1 cut(s)	1376
Ecl136II	GAGCTC	1 cut(s)	1886
EclXI	CGGCCG	1 cut(s)	2195
Eco130I	CCWWGG	3 cut(s)	199, 1582, 2160
Eco24I	GRGCYC	3 cut(s)	434, 1888, 3094
Eco47I	GGWCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
Eco47III	AGCGCT	2 cut(s)	3738, 4065
Eco52I	CGGCCG	1 cut(s)	2195
Eco53kI	GAGCTC	1 cut(s)	1886
Eco57I	CTGAAG	6 cut(s)	1019, 2394, 2916, 2943, 3219, 3894
Eco72I	CACGTG	1 cut(s)	1295
Eco88I	CYCGRG	1 cut(s)	942
EcoICRI	GAGCTC	1 cut(s)	1886
EcoNI	CCTNNNNNAGG	1 cut(s)	1394
EcoO109I	RGGNCCY	2 cut(s)	552, 3271
EcoRII	CCWGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
EcoT14I	CCWWGG	3 cut(s)	199, 1582, 2160
EcoT22I	ATGCAT	2 cut(s)	1753, 2355
EcoT38I	GRGCYC	3 cut(s)	434, 1888, 3094
ErhI	CCWWGG	3 cut(s)	199, 1582, 2160
Esp3I	CGTCTC	1 cut(s)	1473
FalI	AAGNNNNNCTT	4 cut(s)	1811, 1843, 2654, 2686
FaqI	GGGAC	6 cut(s)	965, 1029, 1185, 3176, 3284, 3890
FauI	CCCGC	2 cut(s)	2658, 3081
FauNDI	CATATG	5 cut(s)	448, 1067, 2248, 2328, 2360
FbaI	TGATCA	6 cut(s)	700, 1041, 2728, 3517, 3730, 3976
FblI	GTMKAC	1 cut(s)	2474
FriOI	GRGCYC	3 cut(s)	434, 1888, 3094
GlaI	GCGC	3 cut(s)	256, 3738, 4065
GsaI	CCCAGC	2 cut(s)	1566, 4116
HaeII	RGCGCY	2 cut(s)	3740, 4067
HaeIII	GGCC	6 cut(s)	51, 918, 2197, 2591, 3352, 4086
HapII	CCGG	1 cut(s)	4087
HgaI	GACGC	4 cut(s)	26, 261, 3178, 3986
HhaI	GCGC	3 cut(s)	257, 3739, 4066
Hin1I	GRCGYC	3 cut(s)	37, 272, 3997
Hin6I	GCGC	3 cut(s)	255, 3737, 4064
HinP1I	GCGC	3 cut(s)	255, 3737, 4064
HincII	GTYRAC	2 cut(s)	2094, 2466
HindII	GTYRAC	2 cut(s)	2094, 2466
HindIII	AAGCTT	1 cut(s)	3291
HpaII	CCGG	1 cut(s)	4087
HphI	GGTGA	9 cut(s)	190, 470, 1618, 2228, 2309, 2713, 3141, 3268, 3737
Hpy166II	GTNNAC	9 cut(s)	214, 1028, 1713, 2094, 2466, 2475, 2704, 3380, 4016
Hpy8I	GTNNAC	9 cut(s)	214, 1028, 1713, 2094, 2466, 2475, 2704, 3380, 4016
Hpy99I	CGWCG	1 cut(s)	194
HpyCH4IV	ACGT	8 cut(s)	192, 1272, 1294, 1494, 1516, 1789, 3752, 3982
HpySE526I	ACGT	8 cut(s)	192, 1272, 1294, 1494, 1516, 1789, 3752, 3982
Hsp92I	GRCGYC	3 cut(s)	37, 272, 3997
HspAI	GCGC	3 cut(s)	255, 3737, 4064
KpnI	GGTACC	1 cut(s)	618
Ksp22I	TGATCA	6 cut(s)	700, 1041, 2728, 3517, 3730, 3976
LguI	GCTCTTC	1 cut(s)	17
LmnI	GCTCC	8 cut(s)	1048, 1161, 1454, 1683, 1883, 1937, 1961, 2518
MaeII	ACGT	8 cut(s)	192, 1272, 1294, 1494, 1516, 1789, 3752, 3982
MaeIII	GTNAC	7 cut(s)	482, 932, 979, 1495, 1602, 3001, 3820
MbiI	CCGCTC	1 cut(s)	48
MflI	RGATCY	5 cut(s)	2420, 2912, 3392, 3785, 3950
MhlI	GDGCHC	6 cut(s)	216, 434, 859, 1053, 1888, 3094
MlyI	GAGTC	5 cut(s)	364, 716, 917, 2056, 2786
MmeI	TCCRAC	4 cut(s)	757, 1456, 1737, 2408
Mph1103I	ATGCAT	2 cut(s)	1753, 2355
MroXI	GAANNNNTTC	2 cut(s)	756, 3535
MslI	CAYNNNNRTG	3 cut(s)	2526, 2797, 4046
MspA1I	CMGCKG	3 cut(s)	1144, 1562, 2537
MspI	CCGG	1 cut(s)	4087
MspR9I	CCNGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
Mva1269I	GAATGC	6 cut(s)	889, 2200, 2353, 3285, 3577, 4128
MvaI	CCWGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
MvnI	CGCG	1 cut(s)	255
NdeI	CATATG	5 cut(s)	448, 1067, 2248, 2328, 2360
NmuCI	GTSAC	6 cut(s)	482, 932, 979, 1602, 3001, 3820
NotI	GCGGCCGC	1 cut(s)	2195
NsiI	ATGCAT	2 cut(s)	1753, 2355
NspI	RCATGY	6 cut(s)	1005, 1178, 2525, 3173, 3189, 3412
PagI	TCATGA	1 cut(s)	3895
PciI	ACATGT	1 cut(s)	1001
PciSI	GCTCTTC	1 cut(s)	17
PcsI	WCGNNNNNNNCGW	1 cut(s)	856
PctI	GAATGC	6 cut(s)	889, 2200, 2353, 3285, 3577, 4128
PdmI	GAANNNNTTC	2 cut(s)	756, 3535
PfeI	GAWTC	7 cut(s)	205, 898, 1121, 2057, 3581, 3885, 4145
PflMI	CCANNNNNTGG	1 cut(s)	2183
PfoI	TCCNGGA	3 cut(s)	901, 2315, 3032
Ple19I	CGATCG	1 cut(s)	850
PleI	GAGTC	5 cut(s)	364, 716, 916, 2055, 2785
PmaCI	CACGTG	1 cut(s)	1295
PmlI	CACGTG	1 cut(s)	1295
PpsI	GAGTC	5 cut(s)	364, 716, 916, 2055, 2785
Ppu21I	YACGTR	3 cut(s)	1295, 1517, 1790
PpuMI	RGGWCCY	2 cut(s)	552, 3271
PscI	ACATGT	1 cut(s)	1001
PsiI	TTATAA	1 cut(s)	2135
Psp124BI	GAGCTC	1 cut(s)	1888
Psp1406I	AACGTT	1 cut(s)	1494
Psp5II	RGGWCCY	2 cut(s)	552, 3271
Psp6I	CCWGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
PspCI	CACGTG	1 cut(s)	1295
PspFI	CCCAGC	2 cut(s)	1562, 4112
PspGI	CCWGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
PspPI	GGNCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
PspPPI	RGGWCCY	2 cut(s)	552, 3271
PsrI	GAACNNNNNNTAC	2 cut(s)	952, 984
PstI	CTGCAG	1 cut(s)	45
PstNI	CAGNNNCTG	5 cut(s)	518, 725, 3824, 3993, 4112
PsuI	RGATCY	5 cut(s)	2420, 2912, 3392, 3785, 3950
PvuI	CGATCG	1 cut(s)	850
PvuII	CAGCTG	3 cut(s)	1144, 1562, 2537
RsaI	GTAC	8 cut(s)	247, 616, 1337, 1528, 2405, 3315, 3707, 3838
RsaNI	GTAC	8 cut(s)	246, 615, 1336, 1527, 2404, 3314, 3706, 3837
RseI	CAYNNNNRTG	3 cut(s)	2526, 2797, 4046
SacI	GAGCTC	1 cut(s)	1888
SapI	GCTCTTC	1 cut(s)	17
Sau96I	GGNCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
ScaI	AGTACT	2 cut(s)	247, 3315
SchI	GAGTC	5 cut(s)	364, 716, 917, 2056, 2786
ScrFI	CCNGG	8 cut(s)	808, 903, 1166, 2317, 2388, 2481, 2996, 3034
SduI	GDGCHC	6 cut(s)	216, 434, 859, 1053, 1888, 3094
SfcI	CTRYAG	3 cut(s)	41, 471, 3106
SinI	GGWCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
SmiMI	CAYNNNNRTG	3 cut(s)	2526, 2797, 4046
SmlI	CTYRAG	2 cut(s)	1282, 4133
SmoI	CTYRAG	2 cut(s)	1282, 4133
SspI	AATATT	1 cut(s)	1243
SstI	GAGCTC	1 cut(s)	1888
StyD4I	CCNGG	8 cut(s)	806, 901, 1164, 2315, 2386, 2479, 2994, 3032
StyI	CCWWGG	3 cut(s)	199, 1582, 2160
TaiI	ACGT	8 cut(s)	195, 1275, 1297, 1497, 1519, 1792, 3755, 3985
TaqI	TCGA	7 cut(s)	189, 208, 538, 1732, 1955, 3506, 3682
TatI	WGTACW	6 cut(s)	245, 1526, 2403, 3313, 3705, 3836
TauI	GCSGC	4 cut(s)	51, 2197, 2200, 2591
TfiI	GAWTC	7 cut(s)	205, 898, 1121, 2057, 3581, 3885, 4145
TseFI	GTSAC	6 cut(s)	482, 932, 979, 1602, 3001, 3820
Tsp45I	GTSAC	6 cut(s)	482, 932, 979, 1602, 3001, 3820
TspGWI	ACGGA	7 cut(s)	286, 590, 1265, 1497, 1854, 3267, 3370
Van91I	CCANNNNNTGG	1 cut(s)	2183
VneI	GTGCAC	1 cut(s)	212
VpaK11BI	GGWCC	6 cut(s)	552, 810, 1247, 1402, 1596, 3271
XagI	CCTNNNNNAGG	1 cut(s)	1394
XapI	RAATTY	9 cut(s)	291, 709, 1190, 1442, 2143, 2172, 2320, 2843, 3814
XbaI	TCTAGA	2 cut(s)	2915, 4000
XceI	RCATGY	6 cut(s)	1005, 1178, 2525, 3173, 3189, 3412
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2177, 2184
XmaJI	CCTAGG	1 cut(s)	1582
XmiI	GTMKAC	1 cut(s)	2474
XmnI	GAANNNNTTC	2 cut(s)	756, 3535
ZrmI	AGTACT	2 cut(s)	247, 3315
Zsp2I	ATGCAT	2 cut(s)	1753, 2355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.