Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0001928

Physical Location & Seq

Genomic Coordinates Reverse (-)

31520159 .. 31529802

Gene Structure 9644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G024500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4116 bp

ATGACCATGGAGGAGAAGAAGAGCGGCTCGTGGCGTCTGCAGAGTGGTCAATATCTGGGAGAAGTCTCGGCCCTGTGTTTCCTTCACCTACCTTCTCACCTGTCAATAATAAGGGAGCTTCCTTATTTAGTGGCCGGGTCGTTCGACGTTTTCCAAGGCATTCGCGTCCATGGCATTTGCTGCGGCGCTACTCATACAGATGGCCCTGAGGGAACCGTTGCTTTCGAAATCGCTGTGTTTGGGGAAAGGAGAGTCAAGGTGTTTAGATTGGAGATTAATTTGGGGCAGCAACTCAGCGTTGGTCTGAGGCTGTTGCACTTGTTGCCCAACTTTGGGAATTGGGTCCTGGATGTTTCCTTCATCAAGCATGGGGAAGGTCAGTGTCTTGCTATTGGGTGTAGTGACAACTCTGTTCATCTTTGGGATGTGTCTACTTCTACTGTGGTTCTTCTAGTTCAGCATCCTGAGAGGACTCTTCTGTATTCAATGCGGTTATGGGGTGAAACTCTTGAAGTTCTGCGCATTGCATCCGGTACCATCTATAATGAGATCATTGTTTGGAAAGTGGCTCCTCAGTCTGATGATGCATCATGTTTGACAAGCCAGGTTGAGCACCACTTTGATCAAAGCAATTCACTCTTAAACGGTGTTCAGCTTCATAATTGCCAGTATGAAGCTATTCATATTAGCAAACTTGCTGGACATGAAGGTTCAATTTTTCGCATATCGTGGTCCTCTGATGGATCAAAACTGGTTTCAGTATCTGACGATCGTAGTGCTCGTGTCTGGGCAGTTTGTACTGAAACGATGCATTCTAAGAAGCCAGCAGACTCCATTGGGCTTATGTTATTTGGTCACAGTGCTCGAGTTTGGGACTGCTGCATTTTTGGATTGTTAATTGTCACAGCTGGTGAGGATTGTACTTGTCGTGTGTGGGGCCTGGATGGAAAACACCTAGAGACAATCAAGGAGCACATAGGAAGGGGCATATGGCGATGTTTGTATGATCCGAAATCTTCGCTTCTAATTACTGCTGGCTTTGACTCTGCAATTAAAGTGCATCAGCTGCACATTCCATTCTCTGGGGGATTAAAGGGACATGCAGAGACAAAACAAATCGGTGGAATAGTTACATACACAACTCGTATCCCTACTTTATCCGAGAATATTGGACCTATGGACAGCAAAAGTGAATATGTTCGTTGCTTGCGTTTTACATGTGAGGACACCCTTTATGTTGCCACAAACCATGGTTATATGTACCATGCTAAGCTATTGGATACCGAAGTAGAATGGACAAAACTTGTTCGCGTCAGTGAGGAGGTGCCAATTGTTTGCATGGATTTATTGTCAGAACCATTCAAACTTTCTTCTGGTGTTAAGGATTGGATTGCTGTTGGAGATGGTAAAGGAAACATGACAGTTGTTGGGGTTATATATGGCGCTTGTACCCCTAGAGTTGGTTTTGCCTTTACTTGGTCAGCTGGAATAGAAAGACAACTCCTTGGAGCACATTGGTGCCAGTCACTAGGTTACGGGTTCATCTTCACTGCTGATCCTAGAGGAACATTGAAGCTGTGGAGGCTGTGTGATCACTCTGCAACGAGCTGTGATGTGTCTCTTTTAGCTGAATTCACATCAAGTTTCGGAAGTAGAATAATGTGTTTAGATGCATCTTTGGAGGAAGAGGTATTGGTATGTGGAGATATACGTGGAAATCTTGTTTTGTTCCCTTTGTTGAAGAGTGTGTTGCTGGGCACATTGGTCGCTTCTGATGTTAAAATATCTCCATCAAGTTCTTTCAAAGGAGCTCATGGGATATCAAGCATCTCCAGGGTTACTGTTGCTAGACTAAGTTCTAATCAGATTGAAATATGCTCGACTGGAGCAGATGGGTGTATATGCTATCTGGAATATGACAAAGATAGGAAGGATTTGGAATTTATAGGGATGAAACAAGTAAAGGAGTTGAGTCTTATTCAATCTGTCTCTGCGTGCAATAGCTCTGTGAATGAGCCATCAAACTGTCATTATGCTGCAGGTTTTGCATCAGTGGATTTTATAATTTGGAATTTATTAACTGAGACCAAGGTCTTCCAAATCCCATGTGGTGGATGGCGGCGTCCTCATTCCTATTATCTTGGTGATGTACCAGAGATAAAGAACTGCTTTGCATATGTTAAGGATGATATCATTTATATTCATCGACGCTGGGTACTGAATGGTGAAGGGAAGGAACTCTCTAGGAATTTGCATATGCAATTCCATGGGAGAGAGATGCATTCCATATGCTTTGTCTCAGAAGAATTGCAACCTGGAGTAACTGGGAAGGACCATCTGCTCTATAGATCTAGTTGGATTGCAACTGGCTGTGAAGACGGAACTGTGAGGTTGACTAGGTATATGCCAGATGTTGATAATTGGTCTGGATCAAAATTGCTTGGGGAGCATGTTGGTGGATCAGCTGTGAGATCAATATGCTGTGTGTCAAAGATAAACATACTTCCGTCAGATATGACTGGCATTCCCAATATAAGAACTGCTAACAATGGAACAACAGAGAATAGAGAGACTCCAACTTTATTGATTTCTGTTGGTGCAAAGCGGGTCTTGACTTCTTGGCTACTAAGAAGTAGGAAAGTAGACAAGAAGGAAGAAATAGTACGTGATCCGCAGCATGACAACACTGGAAATGGCAATACATGTTTGTCCCCAGAGTCGCCTTCAATGTCATTCCAGTGGCTTTCTACAGATATGCCAGCAAAATATTCTAGTATTCAAAAAGTTCCAACAAACATCGAGAAAAGAGCTGATCAAGCTGGCGATGCTGATGGTAAAGATGATGCAGCATCAGAAAAGGGAAACAAAGACTCGAACTCCTGCATTAAAGATAAATATGAAGATGACTGGAGATACATGGCTGTTACTGCTTTTCTTGTAAAGTGTGCCAATTCCAGGATAACTGTCTGTTTTATTGGTGTCGCTTGTTCTGATGCCACCCTTGCATTACGGGCTCTAGTTTTGCCCTATAGGCTATGGTTTGATGTTGCTTTTCTGTGTCCTCTATCATCACCAGTTCTGTCCCTACAGCATGTCATCCTTCCTGCATGTCTACCTTCTGAAGGCAATTGGCAGATTGGAAGTTTGTATATTCTCATCAGTGGGGCTACTGATGGAAGTATTGCCTTTTGGGACCTCACTAGAAGCATTGAAGCTTTTATGCAGCTGGTATCAGTGCTTGATACAGAAAAGTTCATTGACTGTCAGAAACGGCCGAGAACAGGGAGGGGAAGTCAGGGTGGAAGACGGTGGAGATCTTTAGACAGTAGCATGTCAAAGAATAGGCTAGGTGCCAGTTCTACTACTGTCAAAGCTGGAGTGGAAACTGATGAGAATTTGCCAAAGCATTCTGGGGATGGAACTTCATCAATGCTAAATGATAATGGGAGCAGTAGAACGGCTTCTTCACATGCTATTCATACTGCTTCCCTAGATTCAGAAGCAAGTGCTTATGATTCCTCATCGGACATATGTGAAATATCACCATTACTTGTCTTCAAGGCCATTCACAAATCTGGTGTCAATTCTCTCCACATTTCAAATGTAGAGGGTTGTCTAAGTCCTGAAATCGGTTTTCTCTATAATTTAATTAGTGGGGGTGATGATCAAGCCCTTAGCTGTCTTACATTTGAATTGTCATTGTCAACATTTAGCTCTGAATTTGATAATATGACACTGGAAATCAAAAATTCAATCACTGAACTTGGAAATGCAAAGAAGTTAGTTCATTCTAACCAAGACAAGAAGAAGAACTATTGGATCCGATTCTTAAATCATGATACAGTCCCCTCAGCTCACAGCTCTGCCGTAAAAGGTGTTTGGACAGACGGATCTTGGGTTTTTTCAACTGGTCTGGATCAGCGTGTCAGGTGCTGGCGTCTTCAGGAGGAAGGTAAATTAATCGAGTATGCTTATTTGGTCATCAGTGTGCCAGAGCCAGAAGCACTGGATGCTAAACTGTGTGGCAGGAACAAATATCAGATTGCTGTAGCTGGGAGGGGAATGCAGATGCTGGAGTTCTCTGAAATTCTGGCATCAGTTGATATAACCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0007049 GO:0007050 GO:0008150 GO:0008285 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010506 GO:0010507 GO:0016020 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0042127 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1371

Amino Acids

151.24

Weight (kDa)

6.09

Isoelectric Point (pI)

42.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	121 - 337	6.2e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	215 - 355	3.8e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	217 - 355	4.6e-13	WDR3 first beta-propeller domain
WD40	PF00400	229 - 263	6.2e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	235 - 353	5.1e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001928)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01860 AT4G01860
fragaria_vesca	FvH4_1g26470 FvH4_2g08080
malus_domestica	MD09G1293000.v1.1 MD17G1281300.v1.1 MD17G1281400.v1.1 MD17G1281500.v1.1 MD17G1281700.v1.1 MD17G1281800.v1.1
prunus_persica	Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1
pyrus_communis	pycom09g19610 pycom09g19620 pycom09g19630 pycom17g27900
rosa_chinensis	RchiOBHm_Chr3g0486381 RchiOBHm_Chr3g0486491 RchiOBHm_Chr3g0486501 RchiOBHm_Chr3g0486511
rosa_laevigata	RLG00000023079 RLG00000023084 RLG00000023086 RLG00000023087
rosa_multiflora	Rmu_sc0001857.1_g000004 Rmu_sc0003801.1_g000001 Rmu_sc0003801.1_g000026 Rmu_sc0011408.1_g000006
rosa_roxburghii	Rroxscaffold_1G00022380 Rroxscaffold_3G00237910 Rroxscaffold_3G00237930 Rroxscaffold_3G00237990 Rroxscaffold_6G00395820
rosa_rugosa	Rorug03G0224900
rosa_samantha	Rh3AG274900 Rh3BG310000 Rh3CG309200 Rh3DG305300 Rh3DG306000
rosa_wichuraiana	Rw3G024390 Rw3G024500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2063
Acc16I	TGCGCA	1 cut(s)	521
Acc36I	ACCTGC	1 cut(s)	2030
Acc65I	GGTACC	1 cut(s)	533
AccB1I	GGYRCC	4 cut(s)	533, 1324, 1518, 3350
AccB7I	CCANNNNNTGG	2 cut(s)	1082, 2111
AccBSI	CCGCTC	1 cut(s)	24
AccI	GTMKAC	3 cut(s)	431, 2643, 3112
AccII	CGCG	2 cut(s)	165, 1311
AciI	CCGC	6 cut(s)	24, 183, 490, 2119, 2605, 2672
AcoI	YGGCCR	2 cut(s)	132, 3272
AcsI	RAATTY	8 cut(s)	1631, 1940, 2071, 2248, 3394, 3719, 3748, 4086
AcuI	CTGAAG	2 cut(s)	3141, 3926
AcyI	GRCGYC	3 cut(s)	34, 2122, 3937
AfaI	GTAC	8 cut(s)	535, 799, 922, 1262, 1450, 2151, 2217, 2664
AflIII	ACRYGT	2 cut(s)	1217, 2702
AjnI	CCWGG	6 cut(s)	345, 603, 939, 1832, 2314, 2954
AleI	CACNNNNGTG	1 cut(s)	1516
Alw21I	GWGCWC	6 cut(s)	615, 781, 865, 975, 1513, 1813
Alw26I	GTCTC	8 cut(s)	70, 953, 1100, 1623, 1993, 2078, 2302, 2564
AlwNI	CAGNNNCTG	3 cut(s)	764, 3933, 4072
Ama87I	CYCGRG	1 cut(s)	864
AoxI	GGCC	6 cut(s)	69, 132, 202, 937, 3272, 3561
ApeKI	GCWGC	8 cut(s)	180, 286, 879, 1066, 2036, 2674, 2846, 3223
ApoI	RAATTY	8 cut(s)	1631, 1940, 2071, 2248, 3394, 3719, 3748, 4086
ArsI	GACNNNNNNTTYG	2 cut(s)	3564, 3596
AseI	ATTAAT	2 cut(s)	276, 3959
Asp700I	GAANNNNTTC	3 cut(s)	678, 1197, 3460
Asp718I	GGTACC	1 cut(s)	533
AspLEI	GCGC	3 cut(s)	188, 522, 1445
AspS9I	GGNCC	8 cut(s)	70, 203, 343, 732, 937, 1172, 2332, 3193
AsuC2I	CCSGG	1 cut(s)	136
AsuHPI	GGTGA	9 cut(s)	77, 89, 512, 923, 2156, 2237, 3063, 3534, 3671
AsuII	TTCGAA	1 cut(s)	225
AvaI	CYCGRG	1 cut(s)	864
AvaII	GGWCC	5 cut(s)	343, 732, 1172, 2332, 3193
AxyI	CCTNAGG	1 cut(s)	207
BaeGI	GKGCMC	1 cut(s)	1760
BaeI	ACNNNNGTAYC	2 cut(s)	1272, 1305
BamHI	GGATCC	1 cut(s)	3819
BanI	GGYRCC	4 cut(s)	533, 1324, 1518, 3350
BanII	GRGCYC	2 cut(s)	1813, 3016
BarI	GAAGNNNNNNTAC	4 cut(s)	2646, 2678, 3445, 3477
BauI	CACGAG	2 cut(s)	28, 780
BbsI	GAAGAC	5 cut(s)	2086, 2382, 3310, 3547, 3932
Bbv12I	GWGCWC	6 cut(s)	615, 781, 865, 975, 1513, 1813
BbvCI	CCTCAGC	1 cut(s)	3850
BbvI	GCAGC	8 cut(s)	167, 298, 866, 1053, 2023, 2686, 2858, 3235
BceAI	ACGGC	3 cut(s)	3287, 3474, 3851
BcgI	CGANNNNNNTGC	4 cut(s)	758, 792, 1747, 1781
BciT130I	CCWGG	6 cut(s)	347, 605, 941, 1834, 2316, 2956
BciVI	GTATCC	2 cut(s)	1157, 1273
BclI	TGATCA	4 cut(s)	622, 1591, 2812, 3664
BcnI	CCSGG	1 cut(s)	136
BcoDI	GTCTC	8 cut(s)	70, 953, 1100, 1623, 1993, 2078, 2302, 2564
BfmI	CTRYAG	7 cut(s)	38, 2037, 2344, 2748, 3028, 3086, 4047
BfoI	RGCGCY	2 cut(s)	189, 1446
BfuAI	ACCTGC	1 cut(s)	2030
BfuI	GTATCC	2 cut(s)	1157, 1273
BglI	GCCNNNNNGGC	1 cut(s)	3031
BglII	AGATCT	2 cut(s)	2348, 3314
BlpI	GCTNAGC	1 cut(s)	1269
Bme1390I	CCNGG	7 cut(s)	136, 347, 605, 941, 1834, 2316, 2956
Bme18I	GGWCC	5 cut(s)	343, 732, 1172, 2332, 3193
BmeT110I	CYCGRG	1 cut(s)	864
BmgT120I	GGNCC	8 cut(s)	70, 203, 343, 732, 937, 1172, 2332, 3193
BmrFI	CCNGG	7 cut(s)	136, 347, 605, 941, 1834, 2316, 2956
BmrI	ACTGGG	2 cut(s)	2334, 4105
BmuI	ACTGGG	2 cut(s)	2334, 4105
BoxI	GACNNNNGTC	1 cut(s)	2090
BpiI	GAAGAC	5 cut(s)	2086, 2382, 3310, 3547, 3932
BpmI	CTGGAG	6 cut(s)	1816, 1905, 2337, 2929, 3396, 4094
Bpu10I	CCTNAGC	2 cut(s)	3674, 3850
Bpu1102I	GCTNAGC	1 cut(s)	1269
Bpu14I	TTCGAA	1 cut(s)	225
BpuMI	CCSGG	1 cut(s)	136
BsaAI	YACGTR	2 cut(s)	1712, 2666
BsaHI	GRCGYC	3 cut(s)	34, 2122, 3937
BsaI	GGTCTC	1 cut(s)	2078
BsaJI	CCNNGG	8 cut(s)	6, 154, 169, 1249, 1504, 1833, 2088, 2266
BsaWI	WCCGGW	1 cut(s)	530
BsaXI	ACNNNNNCTCC	6 cut(s)	1573, 1603, 2861, 2891, 3277, 3307
Bse21I	CCTNAGG	1 cut(s)	207
Bse3DI	GCAATG	1 cut(s)	522
BseBI	CCWGG	6 cut(s)	347, 605, 941, 1834, 2316, 2956
BseDI	CCNNGG	8 cut(s)	6, 154, 169, 1249, 1504, 1833, 2088, 2266
BseMI	GCAATG	1 cut(s)	522
BseMII	CTCAG	8 cut(s)	198, 296, 307, 456, 587, 2073, 2313, 3864
BseRI	GAGGAG	3 cut(s)	26, 561, 1334
BseSI	GKGCMC	1 cut(s)	1760
BseX3I	CGGCCG	1 cut(s)	3272
BseXI	GCAGC	8 cut(s)	167, 298, 866, 1053, 2023, 2686, 2858, 3235
BseYI	CCCAGC	3 cut(s)	1753, 2211, 4052
BsgI	GTGCAG	1 cut(s)	1052
Bsh1236I	CGCG	2 cut(s)	165, 1311
Bsh1285I	CGRYCG	2 cut(s)	772, 3275
BshFI	GGCC	6 cut(s)	71, 134, 204, 939, 3274, 3563
BshNI	GGYRCC	4 cut(s)	533, 1324, 1518, 3350
BsiEI	CGRYCG	2 cut(s)	772, 3275
BsiHKAI	GWGCWC	6 cut(s)	615, 781, 865, 975, 1513, 1813
BsiHKCI	CYCGRG	1 cut(s)	864
BsiSI	CCGG	2 cut(s)	135, 531
BslFI	GGGAC	6 cut(s)	887, 1110, 2695, 3067, 3206, 3830
BsmAI	GTCTC	8 cut(s)	70, 953, 1100, 1623, 1993, 2078, 2302, 2564
BsmFI	GGGAC	6 cut(s)	887, 1110, 2695, 3067, 3206, 3830
BsmI	GAATGC	6 cut(s)	159, 811, 2281, 2523, 3406, 4068
BsnI	GGCC	6 cut(s)	71, 134, 204, 939, 3274, 3563
Bso31I	GGTCTC	1 cut(s)	2078
BsoBI	CYCGRG	1 cut(s)	864
Bsp119I	TTCGAA	1 cut(s)	225
Bsp1286I	GDGCHC	8 cut(s)	615, 781, 865, 975, 1513, 1760, 1813, 3016
Bsp1720I	GCTNAGC	1 cut(s)	1269
Bsp19I	CCATGG	4 cut(s)	6, 169, 1249, 2266
BspACI	CCGC	6 cut(s)	24, 183, 490, 2119, 2605, 2672
BspANI	GGCC	6 cut(s)	71, 134, 204, 939, 3274, 3563
BspCNI	CTCAG	8 cut(s)	199, 297, 306, 457, 586, 2074, 2312, 3863
BspFNI	CGCG	2 cut(s)	165, 1311
BspHI	TCATGA	1 cut(s)	3835
BspMAI	CTGCAG	2 cut(s)	42, 2041
BspMI	ACCTGC	1 cut(s)	2030
BspQI	GCTCTTC	1 cut(s)	14
BspT104I	TTCGAA	1 cut(s)	225
BspT107I	GGYRCC	4 cut(s)	533, 1324, 1518, 3350
BspTNI	GGTCTC	1 cut(s)	2078
BsrBI	CCGCTC	1 cut(s)	24
BsrDI	GCAATG	1 cut(s)	522
BssECI	CCNNGG	8 cut(s)	6, 154, 169, 1249, 1504, 1833, 2088, 2266
BssNI	GRCGYC	3 cut(s)	34, 2122, 3937
BssSI	CACGAG	2 cut(s)	28, 780
BssT1I	CCWWGG	7 cut(s)	6, 154, 169, 1249, 1504, 2088, 2266
Bst2BI	CACGAG	2 cut(s)	28, 780
Bst2UI	CCWGG	6 cut(s)	347, 605, 941, 1834, 2316, 2956
Bst6I	CTCTTC	4 cut(s)	14, 480, 1680, 1736
BstACI	GRCGYC	3 cut(s)	34, 2122, 3937
BstAPI	GCANNNNNTGC	5 cut(s)	180, 322, 1066, 2045, 4010
BstBAI	YACGTR	2 cut(s)	1712, 2666
BstBI	TTCGAA	1 cut(s)	225
BstC8I	GCNNGC	7 cut(s)	825, 1036, 1208, 1996, 2760, 2821, 3935
BstDSI	CCRYGG	4 cut(s)	6, 169, 1249, 2266
BstENI	CCTNNNNNAGG	1 cut(s)	3120
BstFNI	CGCG	2 cut(s)	165, 1311
BstH2I	RGCGCY	2 cut(s)	189, 1446
BstHHI	GCGC	3 cut(s)	188, 522, 1445
BstMAI	GTCTC	8 cut(s)	70, 953, 1100, 1623, 1993, 2078, 2302, 2564
BstMCI	CGRYCG	2 cut(s)	772, 3275
BstNI	CCWGG	6 cut(s)	347, 605, 941, 1834, 2316, 2956
BstNSI	RCATGY	8 cut(s)	1103, 1221, 2453, 2706, 3095, 3111, 3334, 3473
BstPAI	GACNNNNGTC	1 cut(s)	2090
BstSCI	CCNGG	7 cut(s)	134, 345, 603, 939, 1832, 2314, 2954
BstSFI	CTRYAG	7 cut(s)	38, 2037, 2344, 2748, 3028, 3086, 4047
BstSLI	GKGCMC	1 cut(s)	1760
BstUI	CGCG	2 cut(s)	165, 1311
BstV1I	GCAGC	8 cut(s)	167, 298, 866, 1053, 2023, 2686, 2858, 3235
BstV2I	GAAGAC	5 cut(s)	2086, 2382, 3310, 3547, 3932
BstX2I	RGATCY	4 cut(s)	2348, 3314, 3819, 3890
BstYI	RGATCY	4 cut(s)	2348, 3314, 3819, 3890
BstZI	CGGCCG	1 cut(s)	3272
Bsu36I	CCTNAGG	1 cut(s)	207
BsuI	GTATCC	2 cut(s)	1157, 1273
BsuRI	GGCC	6 cut(s)	71, 134, 204, 939, 3274, 3563
BtgI	CCRYGG	4 cut(s)	6, 169, 1249, 2266
BtgZI	GCGATG	2 cut(s)	1009, 2838
BtsI	GCAGTG	1 cut(s)	1548
BveI	ACCTGC	1 cut(s)	2030
Cac8I	GCNNGC	7 cut(s)	825, 1036, 1208, 1996, 2760, 2821, 3935
CaiI	CAGNNNCTG	3 cut(s)	764, 3933, 4072
CciI	TCATGA	1 cut(s)	3835
CfoI	GCGC	3 cut(s)	188, 522, 1445
Cfr13I	GGNCC	8 cut(s)	70, 203, 343, 732, 937, 1172, 2332, 3193
CseI	GACGC	6 cut(s)	23, 154, 1300, 2111, 2217, 3926
Csp6I	GTAC	8 cut(s)	534, 798, 921, 1261, 1449, 2150, 2216, 2663
CviQI	GTAC	8 cut(s)	534, 798, 921, 1261, 1449, 2150, 2216, 2663
EaeI	YGGCCR	2 cut(s)	132, 3272
EagI	CGGCCG	1 cut(s)	3272
Eam1104I	CTCTTC	4 cut(s)	14, 480, 1680, 1736
EarI	CTCTTC	4 cut(s)	14, 480, 1680, 1736
Ecl136II	GAGCTC	1 cut(s)	1811
EclXI	CGGCCG	1 cut(s)	3272
Eco130I	CCWWGG	7 cut(s)	6, 154, 169, 1249, 1504, 2088, 2266
Eco24I	GRGCYC	2 cut(s)	1813, 3016
Eco31I	GGTCTC	1 cut(s)	2078
Eco32I	GATATC	2 cut(s)	1821, 2191
Eco47I	GGWCC	5 cut(s)	343, 732, 1172, 2332, 3193
Eco52I	CGGCCG	1 cut(s)	3272
Eco53kI	GAGCTC	1 cut(s)	1811
Eco57I	CTGAAG	2 cut(s)	3141, 3926
Eco81I	CCTNAGG	1 cut(s)	207
Eco88I	CYCGRG	1 cut(s)	864
EcoICRI	GAGCTC	1 cut(s)	1811
EcoNI	CCTNNNNNAGG	1 cut(s)	3120
EcoO109I	RGGNCCY	3 cut(s)	343, 937, 3193
EcoRI	GAATTC	1 cut(s)	1631
EcoRII	CCWGG	6 cut(s)	345, 603, 939, 1832, 2314, 2954
EcoRV	GATATC	2 cut(s)	1821, 2191
EcoT14I	CCWWGG	7 cut(s)	6, 154, 169, 1249, 1504, 2088, 2266
EcoT22I	ATGCAT	4 cut(s)	589, 813, 1675, 2283
EcoT38I	GRGCYC	2 cut(s)	1813, 3016
ErhI	CCWWGG	7 cut(s)	6, 154, 169, 1249, 1504, 2088, 2266
FalI	AAGNNNNNCTT	4 cut(s)	2153, 2185, 2594, 2626
FaqI	GGGAC	6 cut(s)	887, 1110, 2695, 3067, 3206, 3830
FauI	CCCGC	1 cut(s)	2598
FauNDI	CATATG	5 cut(s)	989, 2176, 2256, 2288, 3530
FbaI	TGATCA	4 cut(s)	622, 1591, 2812, 3664
FblI	GTMKAC	3 cut(s)	431, 2643, 3112
FriOI	GRGCYC	2 cut(s)	1813, 3016
FspI	TGCGCA	1 cut(s)	521
GlaI	GCGC	3 cut(s)	187, 521, 1444
GsaI	CCCAGC	3 cut(s)	1757, 2215, 4056
GsuI	CTGGAG	6 cut(s)	1816, 1905, 2337, 2929, 3396, 4094
HaeII	RGCGCY	2 cut(s)	189, 1446
HaeIII	GGCC	6 cut(s)	71, 134, 204, 939, 3274, 3563
HapII	CCGG	2 cut(s)	135, 531
HgaI	GACGC	6 cut(s)	23, 154, 1300, 2111, 2217, 3926
HhaI	GCGC	3 cut(s)	188, 522, 1445
Hin1I	GRCGYC	3 cut(s)	34, 2122, 3937
Hin6I	GCGC	3 cut(s)	186, 520, 1443
HinP1I	GCGC	3 cut(s)	186, 520, 1443
HincII	GTYRAC	2 cut(s)	2394, 3705
HindII	GTYRAC	2 cut(s)	2394, 3705
HindIII	AAGCTT	1 cut(s)	3213
HpaII	CCGG	2 cut(s)	135, 531
HphI	GGTGA	9 cut(s)	77, 89, 512, 923, 2156, 2237, 3063, 3534, 3671
Hpy166II	GTNNAC	5 cut(s)	432, 2394, 2644, 3113, 3705
Hpy8I	GTNNAC	5 cut(s)	432, 2394, 2644, 3113, 3705
Hpy99I	CGWCG	2 cut(s)	149, 2211
HpyCH4IV	ACGT	3 cut(s)	147, 1711, 2665
HpySE526I	ACGT	3 cut(s)	147, 1711, 2665
Hsp92I	GRCGYC	3 cut(s)	34, 2122, 3937
HspAI	GCGC	3 cut(s)	186, 520, 1443
KpnI	GGTACC	1 cut(s)	537
Ksp22I	TGATCA	4 cut(s)	622, 1591, 2812, 3664
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	8 cut(s)	115, 574, 970, 1508, 1808, 1886, 2446, 3447
Lsp1109I	GCAGC	8 cut(s)	167, 298, 866, 1053, 2023, 2686, 2858, 3235
MaeII	ACGT	3 cut(s)	147, 1711, 2665
MaeIII	GTNAC	9 cut(s)	401, 854, 901, 1129, 1524, 1532, 1837, 2320, 2923
MbiI	CCGCTC	1 cut(s)	24
MfeI	CAATTG	2 cut(s)	1329, 3127
MflI	RGATCY	4 cut(s)	2348, 3314, 3819, 3890
MhlI	GDGCHC	8 cut(s)	615, 781, 865, 975, 1513, 1760, 1813, 3016
MlyI	GAGTC	8 cut(s)	261, 466, 824, 1037, 1981, 2566, 2726, 2864
MmeI	TCCRAC	4 cut(s)	1378, 2336, 2600, 2813
Mph1103I	ATGCAT	4 cut(s)	589, 813, 1675, 2283
MroXI	GAANNNNTTC	3 cut(s)	678, 1197, 3460
MslI	CAYNNNNRTG	5 cut(s)	198, 1516, 2454, 2737, 3986
MspA1I	CMGCKG	5 cut(s)	908, 1066, 1484, 2465, 3226
MspI	CCGG	2 cut(s)	135, 531
MspR9I	CCNGG	7 cut(s)	136, 347, 605, 941, 1834, 2316, 2956
MunI	CAATTG	2 cut(s)	1329, 3127
Mva1269I	GAATGC	6 cut(s)	159, 811, 2281, 2523, 3406, 4068
MvaI	CCWGG	6 cut(s)	347, 605, 941, 1834, 2316, 2956
MvnI	CGCG	2 cut(s)	165, 1311
NciI	CCSGG	1 cut(s)	136
NcoI	CCATGG	4 cut(s)	6, 169, 1249, 2266
NdeI	CATATG	5 cut(s)	989, 2176, 2256, 2288, 3530
NmeAIII	GCCGAG	2 cut(s)	47, 3300
NmuCI	GTSAC	4 cut(s)	401, 854, 901, 1524
NsbI	TGCGCA	1 cut(s)	521
NsiI	ATGCAT	4 cut(s)	589, 813, 1675, 2283
NspI	RCATGY	8 cut(s)	1103, 1221, 2453, 2706, 3095, 3111, 3334, 3473
NspV	TTCGAA	1 cut(s)	225
OliI	CACNNNNGTG	1 cut(s)	1516
PaeR7I	CTCGAG	1 cut(s)	864
PagI	TCATGA	1 cut(s)	3835
PciI	ACATGT	2 cut(s)	1217, 2702
PciSI	GCTCTTC	1 cut(s)	14
PcsI	WCGNNNNNNNCGW	2 cut(s)	778, 1207
PctI	GAATGC	6 cut(s)	159, 811, 2281, 2523, 3406, 4068
PdmI	GAANNNNTTC	3 cut(s)	678, 1197, 3460
PfeI	GAWTC	3 cut(s)	3494, 3515, 3825
PflMI	CCANNNNNTGG	2 cut(s)	1082, 2111
PfoI	TCCNGGA	2 cut(s)	345, 2954
Ple19I	CGATCG	1 cut(s)	772
PleI	GAGTC	8 cut(s)	260, 466, 824, 1037, 1980, 2566, 2725, 2864
PpsI	GAGTC	8 cut(s)	260, 466, 824, 1037, 1980, 2566, 2725, 2864
Ppu21I	YACGTR	2 cut(s)	1712, 2666
PpuMI	RGGWCCY	2 cut(s)	343, 3193
PscI	ACATGT	2 cut(s)	1217, 2702
PshAI	GACNNNNGTC	1 cut(s)	2090
PshBI	ATTAAT	2 cut(s)	276, 3959
PsiI	TTATAA	1 cut(s)	2063
Psp124BI	GAGCTC	1 cut(s)	1813
Psp5II	RGGWCCY	2 cut(s)	343, 3193
Psp6I	CCWGG	6 cut(s)	345, 603, 939, 1832, 2314, 2954
PspFI	CCCAGC	3 cut(s)	1753, 2211, 4052
PspGI	CCWGG	6 cut(s)	345, 603, 939, 1832, 2314, 2954
PspPI	GGNCC	8 cut(s)	70, 203, 343, 732, 937, 1172, 2332, 3193
PspPPI	RGGWCCY	2 cut(s)	343, 3193
PspXI	VCTCGAGB	1 cut(s)	864
PstI	CTGCAG	2 cut(s)	42, 2041
PstNI	CAGNNNCTG	3 cut(s)	764, 3933, 4072
PsuI	RGATCY	4 cut(s)	2348, 3314, 3819, 3890
PvuI	CGATCG	1 cut(s)	772
PvuII	CAGCTG	5 cut(s)	908, 1066, 1484, 2465, 3226
RsaI	GTAC	8 cut(s)	535, 799, 922, 1262, 1450, 2151, 2217, 2664
RsaNI	GTAC	8 cut(s)	534, 798, 921, 1261, 1449, 2150, 2216, 2663
RseI	CAYNNNNRTG	5 cut(s)	198, 1516, 2454, 2737, 3986
SacI	GAGCTC	1 cut(s)	1813
SapI	GCTCTTC	1 cut(s)	14
Sau96I	GGNCC	8 cut(s)	70, 203, 343, 732, 937, 1172, 2332, 3193
SchI	GAGTC	8 cut(s)	261, 466, 824, 1037, 1981, 2566, 2726, 2864
ScrFI	CCNGG	7 cut(s)	136, 347, 605, 941, 1834, 2316, 2956
SduI	GDGCHC	8 cut(s)	615, 781, 865, 975, 1513, 1760, 1813, 3016
SfcI	CTRYAG	7 cut(s)	38, 2037, 2344, 2748, 3028, 3086, 4047
Sfr274I	CTCGAG	1 cut(s)	864
SfuI	TTCGAA	1 cut(s)	225
SinI	GGWCC	5 cut(s)	343, 732, 1172, 2332, 3193
SlaI	CTCGAG	1 cut(s)	864
SmiMI	CAYNNNNRTG	5 cut(s)	198, 1516, 2454, 2737, 3986
SmlI	CTYRAG	1 cut(s)	864
SmoI	CTYRAG	1 cut(s)	864
SsiI	CCGC	6 cut(s)	24, 183, 490, 2119, 2605, 2672
SspI	AATATT	2 cut(s)	1168, 2768
SstI	GAGCTC	1 cut(s)	1813
StyD4I	CCNGG	7 cut(s)	134, 345, 603, 939, 1832, 2314, 2954
StyI	CCWWGG	7 cut(s)	6, 154, 169, 1249, 1504, 2088, 2266
TaiI	ACGT	3 cut(s)	150, 1714, 2668
TaqI	TCGA	8 cut(s)	144, 225, 865, 1880, 2206, 2799, 2873, 3963
TatI	WGTACW	2 cut(s)	797, 920
TauI	GCSGC	3 cut(s)	27, 186, 2122
TfiI	GAWTC	3 cut(s)	3494, 3515, 3825
TseFI	GTSAC	4 cut(s)	401, 854, 901, 1524
TseI	GCWGC	8 cut(s)	180, 286, 879, 1066, 2036, 2674, 2846, 3223
Tsp45I	GTSAC	4 cut(s)	401, 854, 901, 1524
TspGWI	ACGGA	3 cut(s)	2394, 2496, 3903
Van91I	CCANNNNNTGG	2 cut(s)	1082, 2111
VpaK11BI	GGWCC	5 cut(s)	343, 732, 1172, 2332, 3193
VspI	ATTAAT	2 cut(s)	276, 3959
XagI	CCTNNNNNAGG	1 cut(s)	3120
XapI	RAATTY	8 cut(s)	1631, 1940, 2071, 2248, 3394, 3719, 3748, 4086
XceI	RCATGY	8 cut(s)	1103, 1221, 2453, 2706, 3095, 3111, 3334, 3473
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2105, 2112
XhoI	CTCGAG	1 cut(s)	864
XmiI	GTMKAC	3 cut(s)	431, 2643, 3112
XmnI	GAANNNNTTC	3 cut(s)	678, 1197, 3460
Zsp2I	ATGCAT	4 cut(s)	589, 813, 1675, 2283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.