Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0001928

Physical Location & Seq

Genomic Coordinates Reverse (-)

31453139 .. 31463101

Gene Structure 9963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG310000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4176 bp

ATGGCTATGGAGAAGAAGAAGAGCGGCTCGTGGCGTCTGCAAAGTGGTCAATATCTTGGAGAAGTGTCAGCCCTCTGTTTCCTTCACCTACCTTCTCACCTGTCAATAAGGGAGCTTCCTTATTTAGTGGCCGGGTCGGGTTCGCAAATCTTGGTCTATGATTTGGAACTGGGAACCATGATGAGGTCGTTCGACGTTTTCCAAGGCATTCGCGTCCATGGGATTTGCTGCGGCGCTACTCATACCGATGGCCCTGAGGGAACCGTTGATTTCGAAATCGCTGTGTTTGGGGAAAGGAGAGTCAAGGTGTTTAGATTGGAGATTAATTTGGGGCAGCAAGTCAGTGTTGGTTTGAGGCTGTTGCACTTGTTGCCCAACTTTGGGAATTGGGTCTTGGATGTTTCCTTCATCATGCATGGGGAAGGTCAGTGTCTTGCTATTGGGTGTAGTGACAACTCTGTCCATCTTTGGGATGTATCTACTTCTACTGTGGTTCTTCAAGTTCAGCATCCTGAGAGGACTCTTCTGTATTCAATGCGGTTATGGGGTGAAACTCTTGAAGTTCTGCGCATTGCATCCGGTACTATCTATAATGAGATCATTGTTTGGAAAGTGGCTCCTCAGTCTAATACATCATGTTTGACAAGCCAAGTAGAACACCGCATTGATCAAAGCAATTCACTCTCAAATAGTGTTCAGCTTCATAATTGCCAGTATGAAGCTATTCATATTAGCAAACTTGGTGGACATGAAGGTTCAATTTTTCGCATATCGTGGTCCTCTAATGGATCAAAACTGGTTTCTGTATCTGACGATCGTAGTGCTCGTGTCTGGGCAGTTTGTACTCAAGCAATGCATTCTAAGAAGCCAGCGGACTCCATTGGGCTTATGTTATTTGGTCACAGTGCCCGAGTTTGGGACTGCTGCATTTTTGGATCGTTAATTGTCACAGCTGGTGAGGATTGTACTTGTCGTGTGTGGGGCCTGGATGGTAAACACCTAGAGACAATCAAGGAGCACACAGGAAGGGGTATATGGCGATGTTTGTATGATCCGAAATCTTCGCTTCTAATTACTGCTGGCTTTGACTCTGCAATTAAAGTGCATCAGCTGCACATTCCATTCTCTTGGGGATTAAAGGGACATGCAGAGACAAAACAAATTGATGGAATAGTTGCATACACAACTCGTATCCCTACTTTATCCGAGAATATTGGACCTATGGACAGCAAAAGTGAATATGTTCGTTGCTTGCGTTTTACATGTGAGGACACCCTTTATGTTGCCACAAACCATGGTTATATGTACCATGCTAAGCTATTGGATACTGAAGTAGAATGGACAAAACTTGTTCGCGTCAGTGAGGAGGTGCCAATTGTTTGCATGGATTTATTGTCAGAACCATTCAAACTTTCTTCTGGTGTTAAGGATTGGATTGCTGTTGGAGATGGTAAAGGAAACATGACAGTTGTTGGGGTTATATATGGTGCTTGTACCCCTAGAGTTGGTTTTGCCTTTACTTGGTCAGCTGGAATAGAAAGACAACTTCTTGGAGCACATTGGTGCCAGTCACTAGGTTACGGGTTCATCTTCACTGCTGATCCTAGAGGAACATTGAAGCTGTGGAGGCTGTGTGATCACTCTGCAACGAGCTGTGATGTGTCTCTTTTAGCTGAATTCACATCAAGTTTCGGAAGTAGAATAATGTGTTTAGATGCATCTTTGGAGGAAGAGGTATTGGTATGTGGAGATATACGTGGAAATCTTGTCTTGTTCCCTTTGTTGAAGAGTGTGTTGCTGGGCACATTGGTCGCTTCTGATGTTAAAATATCTCCATCAAGTTGTTTCAAAGGAGCTCATGGGATATCAAGCATCTCCAGTGTTGCTGTTGCTAGACTAAGTTCTAATCAGATTGAAATATGCTCGACTGGAGCAGATGGGTGTATATGCTATCTGGAATATGACAAAGATAGGAAGGATTTGGAATTTATAGGGATGAAACAAGTGAAGGAGTTGAGTCTTATTCAATCTGTCTCTGTGTGCAATAGCTCTGTGAACAAGCCATCAAACTGTCATTATGCTGCTGGTTTTGCATCAGTGGATTTTATAATTTGGAATTTATTAACTGAGACCAAGGTCTTCCAAATCCCATGTGGTGGATGGCGGCGTCCTCATTCCTATTATCTTGGTGATGTACCAGAGATAAAGAACTGCTTTGCATATGTTAAGGATGATATCATTTATATTCATCGATGCTGGGCACTGAATGGTGAAAGGAAGGAACTCTCTAGGAATTTGCATATGCAATTCCATGGGAGAGAGATGCATTCCATATGCTTTGTCTCAGAAGAATTGCAACCTGGAGTAACTGGGAAGGACCATCTGCTCTATAGATCTAGTTGGATTGCAACTGGCTGTGAAGACGGAACTGTGAGGTTGACTAGGTATATGCCAGATGTTGATAATTGGTCTGGATCAAAATTGCTTGGGGAGCATGTTGGTGGATCAGCTGTGAGATCAATATGCTGTGTGTCAAAGATAAACATACTTCCGTCAGATATGACTGGCATTCCCAATATAAGAACTGCTAACAATGGAACAACAGAGAATAGAGAGACTCCGACTTTATTGATTTCTGTTGGTGCAAAGCGGGTCTTGACTTCTTGGCTACTAAGAAGTAGGAAAGTAGACAAGAAGGAAGAAATCGTACGTGATCCGCAGCATGACAACACTGGAAATGGCAATACATGTTTGTCCCCAGAGTCGCCTTCAATGTCATTCCAGTGGCTTTCTACAGATATGCCAGCAAAATATTCTAGTTTTCAAAAAGTTCCAACAAACATCGAGAAAAGAGCTGATCAAGCTGGCGATGCTGATGGTAAAGATGATGCAGCATCAGAAAAGGGAAACAAAGAGTCGAACTCCTGCATTAAAGATAAATATGAAGATGACTGGAGATACATGGCTGTTACTGCTTTTCTTGTAAAGTGTGCCAATTCCAGGATAACTGTCTGTTTTATTGGTGTTGCTTGCTCTGATGCCACCCTTGCATTACGGGCTCTAGTTTTGCCCTATAGGCTATGGTTTGATGTTGCTTTTCTGTGTCCTCTATCATCACCAGTTCTGTCCCTACAGCATGTCATCCTTCCTGCATGTCTACCTTCTGAAGGCAATTGGCAGATTGGAAGTTTGTATTTTCTCATCAGTGGGGCTACTGATGGAAGCATTGCCTTTTGGGACCTCACTAGAAGCATTGAAGCTTTTATGCAGCTGGTGTCAGTACTTAATACAGAAAAGTTCATTGACTGTCAGAAACGGCCGAGAACAGGGAGGGGAAGTCAGGGTGGACGATGGTGGAGATCTTTAGGCAGTAGCATGTCAACGAGTAGGCTAGGTGTCAGTTCCACCACTGTTAAAGCTGGAGTGGAAGCTGATGAGAATTTGCCAAAGCATTCTGGGGATGGAACTTCATCAATGCTAAATGATAATGGGAGCAGTAGAACGGCTTCTTCACATGTTATTCATACTGCTTGCCTAGATTCAGAAGCAAGTGCTTATGATTCCTCATCGGACATATGTGAAATATCACCTTTACTTGTCTTCAAGGCCATTCACCAATCTGGTGTCAATTCTCTCCACATTTCAAATGTAGAGGGTTGTGTACGTCCTGAAATCGGTTTTCTTTATAATTTAATTAGTGGGGGTGATGATCAAGCCCTTAGCTGTCTTACATTTGAATTGTCAGTGTCAACATTTAGCTCTGAATTTGATAATATGACACTGGAAATCAAAAATTCAATCACTGAACTTGGAAATGCAAAGAAGTTAGTTCATTCTAACCAAGACAAGAAGAAGAACTATTGGATCCGATTCTTAAATCATGATAAAGTCCCCTCAGCTCACAGCTCTGCCGTAAAAGGTGTTTGGACAGACGGATCTTGGGTTTTTTCAACTGGTCTGGATCAGCGTGTCAGGTGCTGGCGTCTCCAGGAGGAAGGTAAATTAATCGAGTATGCTTATTTGGTCATCAGTGTGCCAGAGCCAGAAGCACTGGATGCTAAACTGTGTGGCAGGAACAAATATCAGATTGCAGTAGCTGGGAGGGGAATGCAGATGCTGGAGTTCTCTGAAATTCTGGCATCAGTTGATATAACCCAAACTAAAACGATTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0007049 GO:0007050 GO:0008150 GO:0008285 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010506 GO:0010507 GO:0016020 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0042127 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1391

Amino Acids

153.64

Weight (kDa)

6.29

Isoelectric Point (pI)

41.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	139 - 363	2e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	213 - 370	8.6e-14	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	217 - 370	1.6e-13	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	250 - 368	2.3e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001928)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01860 AT4G01860
fragaria_vesca	FvH4_1g26470 FvH4_2g08080
malus_domestica	MD09G1293000.v1.1 MD17G1281300.v1.1 MD17G1281400.v1.1 MD17G1281500.v1.1 MD17G1281700.v1.1 MD17G1281800.v1.1
prunus_persica	Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1 Prupe.3G004200_v2.0.a1
pyrus_communis	pycom09g19610 pycom09g19620 pycom09g19630 pycom17g27900
rosa_chinensis	RchiOBHm_Chr3g0486381 RchiOBHm_Chr3g0486491 RchiOBHm_Chr3g0486501 RchiOBHm_Chr3g0486511
rosa_laevigata	RLG00000023079 RLG00000023084 RLG00000023086 RLG00000023087
rosa_multiflora	Rmu_sc0001857.1_g000004 Rmu_sc0003801.1_g000001 Rmu_sc0003801.1_g000026 Rmu_sc0011408.1_g000006
rosa_roxburghii	Rroxscaffold_1G00022380 Rroxscaffold_3G00237910 Rroxscaffold_3G00237930 Rroxscaffold_3G00237990 Rroxscaffold_6G00395820
rosa_rugosa	Rorug03G0224900
rosa_samantha	Rh3AG274900 Rh3BG310000 Rh3CG309200 Rh3DG305300 Rh3DG306000
rosa_wichuraiana	Rw3G024390 Rw3G024500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2108, 3687
AasI	GACNNNNNNGTC	1 cut(s)	458
Acc16I	TGCGCA	1 cut(s)	569
AccB1I	GGYRCC	2 cut(s)	1369, 1563
AccB7I	CCANNNNNTGG	1 cut(s)	2156
AccBSI	CCGCTC	1 cut(s)	24
AccI	GTMKAC	2 cut(s)	2688, 3157
AccII	CGCG	2 cut(s)	213, 1356
AciI	CCGC	8 cut(s)	24, 231, 538, 661, 872, 2164, 2650, 2717
AcoI	YGGCCR	2 cut(s)	129, 3317
AcsI	RAATTY	8 cut(s)	1676, 1985, 2116, 2293, 3439, 3764, 3793, 4131
AcuI	CTGAAG	2 cut(s)	1350, 3186
AcyI	GRCGYC	3 cut(s)	34, 2167, 3982
AfaI	GTAC	9 cut(s)	583, 844, 967, 1307, 1495, 2196, 2709, 3282, 3663
AflIII	ACRYGT	3 cut(s)	1262, 2747, 3514
AjnI	CCWGG	4 cut(s)	984, 2359, 2999, 3987
AjuI	GAANNNNNNNTTGG	2 cut(s)	377, 409
AleI	CACNNNNGTG	1 cut(s)	1561
Alw21I	GWGCWC	4 cut(s)	826, 1020, 1558, 1858
Alw26I	GTCTC	8 cut(s)	998, 1145, 1668, 2038, 2123, 2347, 2609, 3989
AlwNI	CAGNNNCTG	3 cut(s)	3978, 4097, 4117
Ama87I	CYCGRG	1 cut(s)	909
AoxI	GGCC	5 cut(s)	129, 250, 982, 3317, 3606
ApeKI	GCWGC	8 cut(s)	228, 334, 924, 1111, 2081, 2719, 2891, 3268
ApoI	RAATTY	8 cut(s)	1676, 1985, 2116, 2293, 3439, 3764, 3793, 4131
AseI	ATTAAT	2 cut(s)	324, 4004
Asp700I	GAANNNNTTC	3 cut(s)	723, 1242, 3505
AspLEI	GCGC	2 cut(s)	236, 570
AspS9I	GGNCC	6 cut(s)	251, 777, 982, 1217, 2377, 3238
AsuC2I	CCSGG	1 cut(s)	133
AsuII	TTCGAA	1 cut(s)	273
AvaI	CYCGRG	1 cut(s)	909
AvaII	GGWCC	4 cut(s)	777, 1217, 2377, 3238
AxyI	CCTNAGG	1 cut(s)	255
BaeGI	GKGCMC	3 cut(s)	910, 1805, 2263
BaeI	ACNNNNGTAYC	2 cut(s)	1317, 1350
BamHI	GGATCC	1 cut(s)	3864
BanI	GGYRCC	2 cut(s)	1369, 1563
BanII	GRGCYC	2 cut(s)	1858, 3061
BarI	GAAGNNNNNNTAC	4 cut(s)	2691, 2723, 3490, 3522
BauI	CACGAG	2 cut(s)	28, 825
BbsI	GAAGAC	3 cut(s)	2131, 2427, 3592
Bbv12I	GWGCWC	4 cut(s)	826, 1020, 1558, 1858
BbvCI	CCTCAGC	1 cut(s)	3895
BbvI	GCAGC	8 cut(s)	215, 346, 911, 1098, 2068, 2731, 2903, 3280
BceAI	ACGGC	3 cut(s)	3332, 3519, 3896
BcgI	CGANNNNNNTGC	4 cut(s)	803, 837, 1792, 1826
BciT130I	CCWGG	4 cut(s)	986, 2361, 3001, 3989
BciVI	GTATCC	2 cut(s)	1202, 1318
BclI	TGATCA	4 cut(s)	667, 1636, 2857, 3709
BcnI	CCSGG	1 cut(s)	133
BcoDI	GTCTC	8 cut(s)	998, 1145, 1668, 2038, 2123, 2347, 2609, 3989
BfmI	CTRYAG	4 cut(s)	2389, 2793, 3073, 3131
BfoI	RGCGCY	1 cut(s)	237
BfuI	GTATCC	2 cut(s)	1202, 1318
BglI	GCCNNNNNGGC	1 cut(s)	3076
BglII	AGATCT	2 cut(s)	2393, 3359
BlpI	GCTNAGC	1 cut(s)	1314
BmcAI	AGTACT	1 cut(s)	3282
Bme1390I	CCNGG	5 cut(s)	133, 986, 2361, 3001, 3989
Bme18I	GGWCC	4 cut(s)	777, 1217, 2377, 3238
BmeT110I	CYCGRG	1 cut(s)	909
BmgT120I	GGNCC	6 cut(s)	251, 777, 982, 1217, 2377, 3238
BmiI	GGNNCC	8 cut(s)	175, 262, 618, 983, 1371, 1565, 3239, 3866
BmrFI	CCNGG	5 cut(s)	133, 986, 2361, 3001, 3989
BmrI	ACTGGG	2 cut(s)	179, 2379
BmuI	ACTGGG	2 cut(s)	179, 2379
BoxI	GACNNNNGTC	1 cut(s)	2135
BpiI	GAAGAC	3 cut(s)	2131, 2427, 3592
BplI	GAGNNNNNCTC	2 cut(s)	2906, 2938
BpmI	CTGGAG	7 cut(s)	1861, 1950, 2382, 2974, 3441, 3971, 4139
Bpu10I	CCTNAGC	2 cut(s)	3719, 3895
Bpu1102I	GCTNAGC	1 cut(s)	1314
Bpu14I	TTCGAA	1 cut(s)	273
BpuEI	CTTGAG	1 cut(s)	831
BpuMI	CCSGG	1 cut(s)	133
Bsa29I	ATCGAT	1 cut(s)	2251
BsaAI	YACGTR	2 cut(s)	1757, 2711
BsaHI	GRCGYC	3 cut(s)	34, 2167, 3982
BsaI	GGTCTC	1 cut(s)	2123
BsaJI	CCNNGG	5 cut(s)	202, 217, 1294, 2133, 2311
BsaWI	WCCGGW	1 cut(s)	578
BsaXI	ACNNNNNCTCC	4 cut(s)	1618, 1648, 3322, 3352
Bse21I	CCTNAGG	1 cut(s)	255
Bse3DI	GCAATG	3 cut(s)	570, 858, 3225
BseBI	CCWGG	4 cut(s)	986, 2361, 3001, 3989
BseCI	ATCGAT	1 cut(s)	2251
BseDI	CCNNGG	5 cut(s)	202, 217, 1294, 2133, 2311
BseMI	GCAATG	3 cut(s)	570, 858, 3225
BseMII	CTCAG	6 cut(s)	246, 504, 635, 2118, 2358, 3909
BseRI	GAGGAG	2 cut(s)	609, 1379
BseSI	GKGCMC	3 cut(s)	910, 1805, 2263
BseX3I	CGGCCG	1 cut(s)	3317
BseXI	GCAGC	8 cut(s)	215, 346, 911, 1098, 2068, 2731, 2903, 3280
BseYI	CCCAGC	3 cut(s)	1798, 2256, 4097
BsgI	GTGCAG	1 cut(s)	1097
Bsh1236I	CGCG	2 cut(s)	213, 1356
Bsh1285I	CGRYCG	2 cut(s)	817, 3320
BshFI	GGCC	5 cut(s)	131, 252, 984, 3319, 3608
BshNI	GGYRCC	2 cut(s)	1369, 1563
BshVI	ATCGAT	1 cut(s)	2251
BsiEI	CGRYCG	2 cut(s)	817, 3320
BsiHKAI	GWGCWC	4 cut(s)	826, 1020, 1558, 1858
BsiHKCI	CYCGRG	1 cut(s)	909
BsiSI	CCGG	2 cut(s)	132, 579
BsiWI	CGTACG	1 cut(s)	2707
BslFI	GGGAC	6 cut(s)	932, 1155, 2740, 3112, 3251, 3875
BsmAI	GTCTC	8 cut(s)	998, 1145, 1668, 2038, 2123, 2347, 2609, 3989
BsmBI	CGTCTC	1 cut(s)	3989
BsmFI	GGGAC	6 cut(s)	932, 1155, 2740, 3112, 3251, 3875
BsmI	GAATGC	6 cut(s)	207, 856, 2326, 2568, 3451, 4113
BsnI	GGCC	5 cut(s)	131, 252, 984, 3319, 3608
Bso31I	GGTCTC	1 cut(s)	2123
BsoBI	CYCGRG	1 cut(s)	909
Bsp119I	TTCGAA	1 cut(s)	273
Bsp1286I	GDGCHC	8 cut(s)	826, 910, 1020, 1558, 1805, 1858, 2263, 3061
Bsp1720I	GCTNAGC	1 cut(s)	1314
Bsp19I	CCATGG	3 cut(s)	217, 1294, 2311
BspACI	CCGC	8 cut(s)	24, 231, 538, 661, 872, 2164, 2650, 2717
BspANI	GGCC	5 cut(s)	131, 252, 984, 3319, 3608
BspCNI	CTCAG	6 cut(s)	247, 505, 634, 2119, 2357, 3908
BspDI	ATCGAT	1 cut(s)	2251
BspFNI	CGCG	2 cut(s)	213, 1356
BspHI	TCATGA	1 cut(s)	3880
BspLI	GGNNCC	8 cut(s)	175, 262, 618, 983, 1371, 1565, 3239, 3866
BspQI	GCTCTTC	1 cut(s)	14
BspT104I	TTCGAA	1 cut(s)	273
BspT107I	GGYRCC	2 cut(s)	1369, 1563
BspTNI	GGTCTC	1 cut(s)	2123
BsrBI	CCGCTC	1 cut(s)	24
BsrDI	GCAATG	3 cut(s)	570, 858, 3225
BssECI	CCNNGG	5 cut(s)	202, 217, 1294, 2133, 2311
BssNI	GRCGYC	3 cut(s)	34, 2167, 3982
BssSI	CACGAG	2 cut(s)	28, 825
BssT1I	CCWWGG	5 cut(s)	202, 217, 1294, 2133, 2311
Bst2BI	CACGAG	2 cut(s)	28, 825
Bst2UI	CCWGG	4 cut(s)	986, 2361, 3001, 3989
Bst6I	CTCTTC	4 cut(s)	14, 528, 1725, 1781
BstACI	GRCGYC	3 cut(s)	34, 2167, 3982
BstAPI	GCANNNNNTGC	3 cut(s)	370, 1111, 4055
BstBAI	YACGTR	2 cut(s)	1757, 2711
BstBI	TTCGAA	1 cut(s)	273
BstC8I	GCNNGC	8 cut(s)	870, 1081, 1253, 2805, 2866, 3031, 3532, 3980
BstDSI	CCRYGG	3 cut(s)	217, 1294, 2311
BstENI	CCTNNNNNAGG	1 cut(s)	3165
BstFNI	CGCG	2 cut(s)	213, 1356
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	2 cut(s)	236, 570
BstMAI	GTCTC	8 cut(s)	998, 1145, 1668, 2038, 2123, 2347, 2609, 3989
BstMCI	CGRYCG	2 cut(s)	817, 3320
BstNI	CCWGG	4 cut(s)	986, 2361, 3001, 3989
BstNSI	RCATGY	8 cut(s)	1148, 1266, 2498, 2751, 3140, 3156, 3379, 3518
BstPAI	GACNNNNGTC	1 cut(s)	2135
BstSCI	CCNGG	5 cut(s)	131, 984, 2359, 2999, 3987
BstSFI	CTRYAG	4 cut(s)	2389, 2793, 3073, 3131
BstSLI	GKGCMC	3 cut(s)	910, 1805, 2263
BstUI	CGCG	2 cut(s)	213, 1356
BstV1I	GCAGC	8 cut(s)	215, 346, 911, 1098, 2068, 2731, 2903, 3280
BstV2I	GAAGAC	3 cut(s)	2131, 2427, 3592
BstX2I	RGATCY	4 cut(s)	2393, 3359, 3864, 3935
BstXI	CCANNNNNNTGG	1 cut(s)	1128
BstYI	RGATCY	4 cut(s)	2393, 3359, 3864, 3935
BstZI	CGGCCG	1 cut(s)	3317
Bsu15I	ATCGAT	1 cut(s)	2251
Bsu36I	CCTNAGG	1 cut(s)	255
BsuI	GTATCC	2 cut(s)	1202, 1318
BsuRI	GGCC	5 cut(s)	131, 252, 984, 3319, 3608
BsuTUI	ATCGAT	1 cut(s)	2251
BtgI	CCRYGG	3 cut(s)	217, 1294, 2311
BtgZI	GCGATG	2 cut(s)	1054, 2883
BtsI	GCAGTG	1 cut(s)	1593
Cac8I	GCNNGC	8 cut(s)	870, 1081, 1253, 2805, 2866, 3031, 3532, 3980
CaiI	CAGNNNCTG	3 cut(s)	3978, 4097, 4117
CciI	TCATGA	1 cut(s)	3880
CfoI	GCGC	2 cut(s)	236, 570
Cfr13I	GGNCC	6 cut(s)	251, 777, 982, 1217, 2377, 3238
ClaI	ATCGAT	1 cut(s)	2251
CseI	GACGC	5 cut(s)	23, 202, 1345, 2156, 3971
Csp6I	GTAC	9 cut(s)	582, 843, 966, 1306, 1494, 2195, 2708, 3281, 3662
CspCI	CAANNNNNGTGG	2 cut(s)	724, 759
CviQI	GTAC	9 cut(s)	582, 843, 966, 1306, 1494, 2195, 2708, 3281, 3662
DrdI	GACNNNNNNGTC	1 cut(s)	458
DseDI	GACNNNNNNGTC	1 cut(s)	458
EaeI	YGGCCR	2 cut(s)	129, 3317
EagI	CGGCCG	1 cut(s)	3317
Eam1104I	CTCTTC	4 cut(s)	14, 528, 1725, 1781
EarI	CTCTTC	4 cut(s)	14, 528, 1725, 1781
Ecl136II	GAGCTC	1 cut(s)	1856
EclXI	CGGCCG	1 cut(s)	3317
Eco130I	CCWWGG	5 cut(s)	202, 217, 1294, 2133, 2311
Eco24I	GRGCYC	2 cut(s)	1858, 3061
Eco31I	GGTCTC	1 cut(s)	2123
Eco32I	GATATC	2 cut(s)	1866, 2236
Eco47I	GGWCC	4 cut(s)	777, 1217, 2377, 3238
Eco52I	CGGCCG	1 cut(s)	3317
Eco53kI	GAGCTC	1 cut(s)	1856
Eco57I	CTGAAG	2 cut(s)	1350, 3186
Eco81I	CCTNAGG	1 cut(s)	255
Eco88I	CYCGRG	1 cut(s)	909
EcoICRI	GAGCTC	1 cut(s)	1856
EcoNI	CCTNNNNNAGG	1 cut(s)	3165
EcoO109I	RGGNCCY	2 cut(s)	982, 3238
EcoRI	GAATTC	1 cut(s)	1676
EcoRII	CCWGG	4 cut(s)	984, 2359, 2999, 3987
EcoRV	GATATC	2 cut(s)	1866, 2236
EcoT14I	CCWWGG	5 cut(s)	202, 217, 1294, 2133, 2311
EcoT22I	ATGCAT	4 cut(s)	417, 858, 1720, 2328
EcoT38I	GRGCYC	2 cut(s)	1858, 3061
ErhI	CCWWGG	5 cut(s)	202, 217, 1294, 2133, 2311
Esp3I	CGTCTC	1 cut(s)	3989
FalI	AAGNNNNNCTT	4 cut(s)	2198, 2230, 2639, 2671
FaqI	GGGAC	6 cut(s)	932, 1155, 2740, 3112, 3251, 3875
FauI	CCCGC	1 cut(s)	2643
FauNDI	CATATG	4 cut(s)	2221, 2301, 2333, 3575
FbaI	TGATCA	4 cut(s)	667, 1636, 2857, 3709
FblI	GTMKAC	2 cut(s)	2688, 3157
FriOI	GRGCYC	2 cut(s)	1858, 3061
FspI	TGCGCA	1 cut(s)	569
GlaI	GCGC	2 cut(s)	235, 569
GsaI	CCCAGC	3 cut(s)	1802, 2260, 4101
GsuI	CTGGAG	7 cut(s)	1861, 1950, 2382, 2974, 3441, 3971, 4139
HaeII	RGCGCY	1 cut(s)	237
HaeIII	GGCC	5 cut(s)	131, 252, 984, 3319, 3608
HapII	CCGG	2 cut(s)	132, 579
HgaI	GACGC	5 cut(s)	23, 202, 1345, 2156, 3971
HhaI	GCGC	2 cut(s)	236, 570
Hin1I	GRCGYC	3 cut(s)	34, 2167, 3982
Hin6I	GCGC	2 cut(s)	234, 568
HinP1I	GCGC	2 cut(s)	234, 568
HincII	GTYRAC	3 cut(s)	2439, 3381, 3750
HindII	GTYRAC	3 cut(s)	2439, 3381, 3750
HindIII	AAGCTT	1 cut(s)	3258
HpaII	CCGG	2 cut(s)	132, 579
Hpy188III	TCNNGA	9 cut(s)	512, 557, 1955, 2472, 2656, 2845, 3668, 3881, 3959
Hpy99I	CGWCG	1 cut(s)	197
HpyCH4IV	ACGT	4 cut(s)	195, 1756, 2710, 3664
HpySE526I	ACGT	4 cut(s)	195, 1756, 2710, 3664
Hsp92I	GRCGYC	3 cut(s)	34, 2167, 3982
HspAI	GCGC	2 cut(s)	234, 568
Ksp22I	TGATCA	4 cut(s)	667, 1636, 2857, 3709
LguI	GCTCTTC	1 cut(s)	14
LmnI	GCTCC	8 cut(s)	112, 622, 1015, 1553, 1853, 1931, 2491, 3492
Lsp1109I	GCAGC	8 cut(s)	215, 346, 911, 1098, 2068, 2731, 2903, 3280
MaeII	ACGT	4 cut(s)	195, 1756, 2710, 3664
MaeIII	GTNAC	7 cut(s)	449, 899, 946, 1569, 1577, 2365, 2968
MbiI	CCGCTC	1 cut(s)	24
MfeI	CAATTG	2 cut(s)	1374, 3172
MflI	RGATCY	4 cut(s)	2393, 3359, 3864, 3935
MhlI	GDGCHC	8 cut(s)	826, 910, 1020, 1558, 1805, 1858, 2263, 3061
MlyI	GAGTC	8 cut(s)	309, 514, 869, 1082, 2026, 2611, 2771, 2924
MmeI	TCCRAC	4 cut(s)	1423, 2381, 2645, 2858
Mph1103I	ATGCAT	4 cut(s)	417, 858, 1720, 2328
MroXI	GAANNNNTTC	3 cut(s)	723, 1242, 3505
MslI	CAYNNNNRTG	5 cut(s)	246, 1561, 2499, 2782, 4031
MspA1I	CMGCKG	6 cut(s)	872, 953, 1111, 1529, 2510, 3271
MspI	CCGG	2 cut(s)	132, 579
MspR9I	CCNGG	5 cut(s)	133, 986, 2361, 3001, 3989
MunI	CAATTG	2 cut(s)	1374, 3172
Mva1269I	GAATGC	6 cut(s)	207, 856, 2326, 2568, 3451, 4113
MvaI	CCWGG	4 cut(s)	986, 2361, 3001, 3989
MvnI	CGCG	2 cut(s)	213, 1356
NciI	CCSGG	1 cut(s)	133
NcoI	CCATGG	3 cut(s)	217, 1294, 2311
NdeI	CATATG	4 cut(s)	2221, 2301, 2333, 3575
NlaIV	GGNNCC	8 cut(s)	175, 262, 618, 983, 1371, 1565, 3239, 3866
NmeAIII	GCCGAG	1 cut(s)	3345
NmuCI	GTSAC	4 cut(s)	449, 899, 946, 1569
NsbI	TGCGCA	1 cut(s)	569
NsiI	ATGCAT	4 cut(s)	417, 858, 1720, 2328
NspI	RCATGY	8 cut(s)	1148, 1266, 2498, 2751, 3140, 3156, 3379, 3518
NspV	TTCGAA	1 cut(s)	273
OliI	CACNNNNGTG	1 cut(s)	1561
PagI	TCATGA	1 cut(s)	3880
PciI	ACATGT	3 cut(s)	1262, 2747, 3514
PciSI	GCTCTTC	1 cut(s)	14
PcsI	WCGNNNNNNNCGW	2 cut(s)	823, 1252
PctI	GAATGC	6 cut(s)	207, 856, 2326, 2568, 3451, 4113
PdmI	GAANNNNTTC	3 cut(s)	723, 1242, 3505
PfeI	GAWTC	3 cut(s)	3539, 3560, 3870
Pfl23II	CGTACG	1 cut(s)	2707
PflMI	CCANNNNNTGG	1 cut(s)	2156
PfoI	TCCNGGA	2 cut(s)	2999, 3987
Ple19I	CGATCG	1 cut(s)	817
PleI	GAGTC	8 cut(s)	308, 514, 869, 1082, 2025, 2611, 2770, 2923
PpsI	GAGTC	8 cut(s)	308, 514, 869, 1082, 2025, 2611, 2770, 2923
Ppu21I	YACGTR	2 cut(s)	1757, 2711
PpuMI	RGGWCCY	1 cut(s)	3238
PscI	ACATGT	3 cut(s)	1262, 2747, 3514
PshAI	GACNNNNGTC	1 cut(s)	2135
PshBI	ATTAAT	2 cut(s)	324, 4004
PsiI	TTATAA	2 cut(s)	2108, 3687
Psp124BI	GAGCTC	1 cut(s)	1858
Psp5II	RGGWCCY	1 cut(s)	3238
Psp6I	CCWGG	4 cut(s)	984, 2359, 2999, 3987
PspFI	CCCAGC	3 cut(s)	1798, 2256, 4097
PspGI	CCWGG	4 cut(s)	984, 2359, 2999, 3987
PspLI	CGTACG	1 cut(s)	2707
PspN4I	GGNNCC	8 cut(s)	175, 262, 618, 983, 1371, 1565, 3239, 3866
PspPI	GGNCC	6 cut(s)	251, 777, 982, 1217, 2377, 3238
PspPPI	RGGWCCY	1 cut(s)	3238
PstNI	CAGNNNCTG	3 cut(s)	3978, 4097, 4117
PsuI	RGATCY	4 cut(s)	2393, 3359, 3864, 3935
PvuI	CGATCG	1 cut(s)	817
PvuII	CAGCTG	5 cut(s)	953, 1111, 1529, 2510, 3271
RsaI	GTAC	9 cut(s)	583, 844, 967, 1307, 1495, 2196, 2709, 3282, 3663
RsaNI	GTAC	9 cut(s)	582, 843, 966, 1306, 1494, 2195, 2708, 3281, 3662
RseI	CAYNNNNRTG	5 cut(s)	246, 1561, 2499, 2782, 4031
SacI	GAGCTC	1 cut(s)	1858
SapI	GCTCTTC	1 cut(s)	14
Sau96I	GGNCC	6 cut(s)	251, 777, 982, 1217, 2377, 3238
ScaI	AGTACT	1 cut(s)	3282
SchI	GAGTC	8 cut(s)	309, 514, 869, 1082, 2026, 2611, 2771, 2924
ScrFI	CCNGG	5 cut(s)	133, 986, 2361, 3001, 3989
SduI	GDGCHC	8 cut(s)	826, 910, 1020, 1558, 1805, 1858, 2263, 3061
SfcI	CTRYAG	4 cut(s)	2389, 2793, 3073, 3131
SfuI	TTCGAA	1 cut(s)	273
SinI	GGWCC	4 cut(s)	777, 1217, 2377, 3238
SmiMI	CAYNNNNRTG	5 cut(s)	246, 1561, 2499, 2782, 4031
SmlI	CTYRAG	1 cut(s)	846
SmoI	CTYRAG	1 cut(s)	846
SsiI	CCGC	8 cut(s)	24, 231, 538, 661, 872, 2164, 2650, 2717
SspI	AATATT	2 cut(s)	1213, 2813
SstI	GAGCTC	1 cut(s)	1858
StyD4I	CCNGG	5 cut(s)	131, 984, 2359, 2999, 3987
StyI	CCWWGG	5 cut(s)	202, 217, 1294, 2133, 2311
TaiI	ACGT	4 cut(s)	198, 1759, 2713, 3667
TaqI	TCGA	7 cut(s)	192, 273, 1925, 2251, 2844, 2918, 4008
TatI	WGTACW	3 cut(s)	842, 965, 3280
TauI	GCSGC	3 cut(s)	27, 234, 2167
TfiI	GAWTC	3 cut(s)	3539, 3560, 3870
TseFI	GTSAC	4 cut(s)	449, 899, 946, 1569
TseI	GCWGC	8 cut(s)	228, 334, 924, 1111, 2081, 2719, 2891, 3268
Tsp45I	GTSAC	4 cut(s)	449, 899, 946, 1569
TspGWI	ACGGA	3 cut(s)	2439, 2541, 3948
Van91I	CCANNNNNTGG	1 cut(s)	2156
VpaK11BI	GGWCC	4 cut(s)	777, 1217, 2377, 3238
VspI	ATTAAT	2 cut(s)	324, 4004
XagI	CCTNNNNNAGG	1 cut(s)	3165
XapI	RAATTY	8 cut(s)	1676, 1985, 2116, 2293, 3439, 3764, 3793, 4131
XceI	RCATGY	8 cut(s)	1148, 1266, 2498, 2751, 3140, 3156, 3379, 3518
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2150, 2157
XmiI	GTMKAC	2 cut(s)	2688, 3157
XmnI	GAANNNNTTC	3 cut(s)	723, 1242, 3505
ZrmI	AGTACT	1 cut(s)	3282
Zsp2I	ATGCAT	4 cut(s)	417, 858, 1720, 2328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.