MD12G1078200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001131
Physical Location & Seq
Reverse (-)
9532026 .. 9535409
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1078200.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGGTCAGGGCCTGAGCTGTGGAGAACCTCGTGAGAGTGGAGTCTTTGGAGCTGTGGAGAATGGGGACCTGGATCTCGTTGGAGCAATGGTGGAAGCTGACCCAAGTGTTTTGGAGCTGAGCAAAGGCCGCAGCCGGCTGTCTGCTCTGCATGTCGCCGCTGCAAATGGCCGGATCGAGGTTCTCTCTATGCTGTTGGATCGGTCTGTTATCAATCCAGATGTGGTGAATCGATACAAACAGACTCCGTTAATGTTGGCTGCGATGAATGGGAATATCACTTGTGTCCAGAAGCTTATCCAAGCGGGAGCAAATATATTGATGTTTGATTCCCTGAACGGAAGAACCTGCCTGCATTACGCTGCATACTATGGCCATTCAGACTGCCTTCAAGCCATTCTCTCAACCGCGCATTCCACCCCTGTTGCAAATTCTTGGGGATTTGCGAGATTTGTGAACATAAGAGATGGAGGTGGTGCAACCCCATTGCATTTAGCTGCCCGCCAAAGAGAGCCAGAGTGCGTTCATGTTCTTTTAGACAATGGGGCTCTTGTTTGTGCTTTAACTGGTGGATATGGCTACCCTGGAAGTACACCACTTCACTTAGCTGCTCGTGGTGGATCCTTAGATTGTGTCAGAGAGTTGCTTGCTTGGGGAGCGGATCGGCTTCAAAAAGACTCTTCTGGGAGAATACCATATACAGTTGCTCTGAAGCACAAGCATCAAGCTTGCGCAGCCGTATTGAACCCTTCATCAGCAGAGCCTCTTGTCTGGCCATCGCCTTTGAAGTTCATCAGTGAGCTCAATCCAGAGGCTAAAGCTTTGTTAGAAAGGGCATTAATGGAGGCAAACATGGAGAGGGAGAAAACTATCTTGAAGGATACAGTTTACTCACTTCCATCTCCTCTGCATTCAGATGCAGAGGCTGATGATAATGCCTCTGAGGCTAGTGATGTGGACCTGTGCTGCATATGCTTTGACCAGTTATGCACAATTGAGGTCGGACCATGTGGTCATCAAATGTGCGCACATTGTACCCTTGCTCTATGCTGCCACAAGAAGCCCGATCCATCATCTACTTGTCCTACAGTCCCAGTTTGCCCCTTTTGCCGAACCACCATTACCCAACTAGTTGTTGCCAACATTAAGGCCAACAATGACATGGATCCAGAAATGAGTCCCGCAAAGCCAAGGAGATCAAGAAAGTCTAATGTCAGTGAAGGCAGAAGTAGCTTCAAGGGGTTGTCTCCCATGAGCTCATTCGGAAGGATGGGAAATCGCAGTTCAGGTAAGGTAGCTGCCGAGTGCAATGAGGAGATCCATAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

444

Amino Acids

47.48

Weight (kDa)

6.4

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 107 4.5e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 50 - 100 1.7e-09 Ankyrin repeats (many copies)
Ank PF00023 81 - 108 8.6e-06 Ankyrin repeat
Ank_4 PF13637 95 - 132 1.5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 111 - 184 3.8e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 196 - 249 6e-07 Ankyrin repeats (3 copies)
Ank PF00023 196 - 226 2e-07 Ankyrin repeat
RING_XB3-XBAT31 PF24921 321 - 376 1e-20 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1011
Acc16I TGCGCA 2 cut(s) 733, 1027
Acc36I ACCTGC 1 cut(s) 356
AccBSI CCGCTC 1 cut(s) 659
AccII CGCG 1 cut(s) 410
AciI CCGC 7 cut(s) 130, 159, 305, 408, 502, 659, 1182
AcoI YGGCCR 3 cut(s) 169, 373, 773
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 731
AfaI GTAC 2 cut(s) 592, 1036
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 6 cut(s) 392, 671, 745, 787, 877, 1237
AhlI ACTAGT 1 cut(s) 1129
AjnI CCWGG 2 cut(s) 69, 583
Alw21I GWGCWC 2 cut(s) 804, 1259
Alw26I GTCTC 1 cut(s) 1251
AlwNI CAGNNNCTG 2 cut(s) 13, 926
AoxI GGCC 6 cut(s) 10, 127, 169, 373, 773, 1149
ApoI RAATTY 1 cut(s) 430
AseI ATTAAT 1 cut(s) 839
AspLEI GCGC 3 cut(s) 412, 734, 1028
AspS9I GGNCC 4 cut(s) 10, 67, 958, 1004
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 3 cut(s) 67, 958, 1004
BalI TGGCCA 2 cut(s) 375, 775
BamHI GGATCC 2 cut(s) 620, 1165
BanII GRGCYC 3 cut(s) 550, 804, 1259
BauI CACGAG 2 cut(s) 30, 612
Bbv12I GWGCWC 2 cut(s) 804, 1259
BccI CCATC 5 cut(s) 461, 784, 907, 1078, 1264
BceAI ACGGC 1 cut(s) 722
BciT130I CCWGG 2 cut(s) 71, 585
BciVI GTATCC 1 cut(s) 874
BcoDI GTCTC 1 cut(s) 1251
BcuI ACTAGT 1 cut(s) 1129
BfaI CTAG 2 cut(s) 948, 1130
BfmI CTRYAG 1 cut(s) 1086
BfuAI ACCTGC 1 cut(s) 356
BfuI GTATCC 1 cut(s) 874
BglI GCCNNNNNGGC 1 cut(s) 944
BlpI GCTNAGC 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 71, 585
Bme18I GGWCC 3 cut(s) 67, 958, 1004
BmgT120I GGNCC 4 cut(s) 10, 67, 958, 1004
BmiI GGNNCC 3 cut(s) 68, 622, 1167
BmrFI CCNGG 2 cut(s) 71, 585
BmrI ACTGGG 1 cut(s) 1088
BmsI GCATC 2 cut(s) 730, 907
BmuI ACTGGG 1 cut(s) 1088
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 583, 1190
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 3 cut(s) 571, 982, 1094
Bse3DI GCAATG 3 cut(s) 93, 485, 1315
BseBI CCWGG 2 cut(s) 71, 585
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 583, 1190
BseGI GGATG 1 cut(s) 1275
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 3 cut(s) 93, 485, 1315
BseMII CTCAG 3 cut(s) 5, 110, 933
BseNI ACTGG 3 cut(s) 571, 982, 1094
BseRI GAGGAG 2 cut(s) 894, 1328
Bsh1236I CGCG 1 cut(s) 410
BshFI GGCC 6 cut(s) 12, 129, 171, 375, 775, 1151
BshVI ATCGAT 1 cut(s) 232
BsiHKAI GWGCWC 2 cut(s) 804, 1259
BsiSI CCGG 2 cut(s) 136, 172
BslFI GGGAC 3 cut(s) 80, 1076, 1164
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 1251
BsmFI GGGAC 3 cut(s) 80, 1076, 1164
BsmI GAATGC 2 cut(s) 412, 910
BsnI GGCC 6 cut(s) 12, 129, 171, 375, 775, 1151
Bsp1286I GDGCHC 3 cut(s) 550, 804, 1259
Bsp143I GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
Bsp1720I GCTNAGC 1 cut(s) 119
BspACI CCGC 7 cut(s) 130, 159, 305, 408, 502, 659, 1182
BspANI GGCC 6 cut(s) 12, 129, 171, 375, 775, 1151
BspCNI CTCAG 3 cut(s) 6, 111, 934
BspDI ATCGAT 1 cut(s) 232
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 3 cut(s) 68, 622, 1167
BspMI ACCTGC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 659
BsrDI GCAATG 3 cut(s) 93, 485, 1315
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 3 cut(s) 571, 982, 1094
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 2 cut(s) 583, 1190
BssMI GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
BssSI CACGAG 2 cut(s) 30, 612
BssT1I CCWWGG 1 cut(s) 1190
Bst2BI CACGAG 2 cut(s) 30, 612
Bst2UI CCWGG 2 cut(s) 71, 585
Bst4CI ACNGT 3 cut(s) 703, 886, 1090
Bst6I CTCTTC 1 cut(s) 685
BstC8I GCNNGC 5 cut(s) 137, 353, 502, 648, 730
BstDEI CTNAG 5 cut(s) 14, 119, 604, 625, 942
BstF5I GGATG 1 cut(s) 1275
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 3 cut(s) 412, 734, 1028
BstKTI GATC 9 cut(s) 76, 177, 202, 623, 664, 1069, 1168, 1199, 1320
BstMAI GTCTC 1 cut(s) 1251
BstMBI GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
BstMWI GCNNNNNNNGC 7 cut(s) 129, 656, 734, 944, 972, 1107, 1230
BstNI CCWGG 2 cut(s) 71, 585
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 69, 583
BstSFI CTRYAG 1 cut(s) 1086
BstUI CGCG 1 cut(s) 410
BstX2I RGATCY 4 cut(s) 73, 620, 1165, 1317
BstYI RGATCY 4 cut(s) 73, 620, 1165, 1317
Bsu15I ATCGAT 1 cut(s) 232
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 6 cut(s) 12, 129, 171, 375, 775, 1151
BsuTUI ATCGAT 1 cut(s) 232
BtgZI GCGATG 2 cut(s) 278, 762
BtsCI GGATG 1 cut(s) 1275
BtsIMutI CAGTG 2 cut(s) 802, 1222
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 5 cut(s) 137, 353, 502, 648, 730
CaiI CAGNNNCTG 2 cut(s) 13, 926
CfoI GCGC 3 cut(s) 412, 734, 1028
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 4 cut(s) 10, 67, 958, 1004
ClaI ATCGAT 1 cut(s) 232
Csp6I GTAC 2 cut(s) 591, 1035
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 6 cut(s) 152, 527, 853, 1008, 1162, 1252
CviQI GTAC 2 cut(s) 591, 1035
DdeI CTNAG 5 cut(s) 14, 119, 604, 625, 942
DpnI GATC 9 cut(s) 75, 176, 201, 622, 663, 1068, 1167, 1198, 1319
DpnII GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
DrdI GACNNNNNNGTC 1 cut(s) 1011
DseDI GACNNNNNNGTC 1 cut(s) 1011
EaeI YGGCCR 3 cut(s) 169, 373, 773
Eam1104I CTCTTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 685
Ecl136II GAGCTC 2 cut(s) 802, 1257
Eco130I CCWWGG 1 cut(s) 1190
Eco24I GRGCYC 3 cut(s) 550, 804, 1259
Eco47I GGWCC 3 cut(s) 67, 958, 1004
Eco53kI GAGCTC 2 cut(s) 802, 1257
Eco57I CTGAAG 1 cut(s) 731
EcoICRI GAGCTC 2 cut(s) 802, 1257
EcoO109I RGGNCCY 2 cut(s) 10, 67
EcoRII CCWGG 2 cut(s) 69, 583
EcoT14I CCWWGG 1 cut(s) 1190
EcoT38I GRGCYC 3 cut(s) 550, 804, 1259
ErhI CCWWGG 1 cut(s) 1190
FaeI CATG 6 cut(s) 155, 530, 856, 1011, 1165, 1255
FaqI GGGAC 3 cut(s) 80, 1076, 1164
FatI CATG 6 cut(s) 151, 526, 852, 1007, 1161, 1251
FauI CCCGC 3 cut(s) 298, 509, 1189
FauNDI CATATG 1 cut(s) 971
FokI GGATG 1 cut(s) 1282
FriOI GRGCYC 3 cut(s) 550, 804, 1259
FspAI RTGCGCAY 1 cut(s) 1027
FspBI CTAG 2 cut(s) 948, 1130
FspI TGCGCA 2 cut(s) 733, 1027
GlaI GCGC 3 cut(s) 411, 733, 1027
HaeIII GGCC 6 cut(s) 12, 129, 171, 375, 775, 1151
HapII CCGG 2 cut(s) 136, 172
HhaI GCGC 3 cut(s) 412, 734, 1028
Hin1II CATG 6 cut(s) 155, 530, 856, 1011, 1165, 1255
Hin6I GCGC 3 cut(s) 410, 732, 1026
HinP1I GCGC 3 cut(s) 410, 732, 1026
HindIII AAGCTT 4 cut(s) 293, 726, 819, 1325
HinfI GANTC 6 cut(s) 42, 229, 244, 329, 677, 1177
HpaII CCGG 2 cut(s) 136, 172
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 4 cut(s) 457, 593, 889, 958
Hpy188I TCNGA 7 cut(s) 382, 638, 711, 916, 943, 1004, 1265
Hpy188III TCNNGA 7 cut(s) 32, 218, 289, 809, 874, 1169, 1200
Hpy8I GTNNAC 4 cut(s) 457, 593, 889, 958
HpyAV CCTTC 5 cut(s) 398, 759, 871, 1214, 1260
HpyCH4III ACNGT 3 cut(s) 703, 886, 1090
HpyF10VI GCNNNNNNNGC 7 cut(s) 129, 656, 734, 944, 972, 1107, 1230
HpyF3I CTNAG 5 cut(s) 14, 119, 604, 625, 942
Hsp92II CATG 6 cut(s) 155, 530, 856, 1011, 1165, 1255
HspAI GCGC 3 cut(s) 410, 732, 1026
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
LmnI GCTCC 5 cut(s) 50, 83, 115, 308, 656
LweI GCATC 2 cut(s) 730, 907
MaeI CTAG 2 cut(s) 948, 1130
MalI GATC 9 cut(s) 75, 176, 201, 622, 663, 1068, 1167, 1198, 1319
MbiI CCGCTC 1 cut(s) 659
MboI GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
MboII GAAGA 2 cut(s) 354, 672
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 4 cut(s) 73, 620, 1165, 1317
MhlI GDGCHC 3 cut(s) 550, 804, 1259
MlsI TGGCCA 2 cut(s) 375, 775
MluCI AATT 2 cut(s) 430, 993
MluNI TGGCCA 2 cut(s) 375, 775
MlyI GAGTC 4 cut(s) 51, 238, 671, 1186
MmeI TCCRAC 3 cut(s) 61, 177, 982
Mox20I TGGCCA 2 cut(s) 375, 775
MroNI GCCGGC 1 cut(s) 135
MscI TGGCCA 2 cut(s) 375, 775
MseI TTAA 4 cut(s) 251, 563, 839, 1146
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 2 cut(s) 375, 775
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 136, 172
MspR9I CCNGG 2 cut(s) 71, 585
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 2 cut(s) 412, 910
MvaI CCWGG 2 cut(s) 71, 585
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 7 cut(s) 129, 656, 734, 944, 972, 1107, 1230
NaeI GCCGGC 1 cut(s) 137
NdeI CATATG 1 cut(s) 971
NdeII GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 6 cut(s) 155, 530, 856, 1011, 1165, 1255
NlaIV GGNNCC 3 cut(s) 68, 622, 1167
NmeAIII GCCGAG 1 cut(s) 1327
NsbI TGCGCA 2 cut(s) 733, 1027
NspI RCATGY 1 cut(s) 155
PctI GAATGC 2 cut(s) 412, 910
PdiI GCCGGC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 229, 329
PleI GAGTC 4 cut(s) 50, 238, 671, 1185
PpsI GAGTC 4 cut(s) 50, 238, 671, 1185
PpuMI RGGWCCY 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 839
Psp124BI GAGCTC 2 cut(s) 804, 1259
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 69, 583
PspGI CCWGG 2 cut(s) 69, 583
PspN4I GGNNCC 3 cut(s) 68, 622, 1167
PspPI GGNCC 4 cut(s) 10, 67, 958, 1004
PspPPI RGGWCCY 1 cut(s) 67
PsrI GAACNNNNNNTAC 2 cut(s) 1105, 1137
PstNI CAGNNNCTG 2 cut(s) 13, 926
PsuI RGATCY 4 cut(s) 73, 620, 1165, 1317
RsaI GTAC 2 cut(s) 592, 1036
RsaNI GTAC 2 cut(s) 591, 1035
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 2 cut(s) 804, 1259
SaqAI TTAA 4 cut(s) 251, 563, 839, 1146
Sau3AI GATC 9 cut(s) 73, 174, 199, 620, 661, 1066, 1165, 1196, 1317
Sau96I GGNCC 4 cut(s) 10, 67, 958, 1004
SchI GAGTC 4 cut(s) 51, 238, 671, 1186
ScrFI CCNGG 2 cut(s) 71, 585
SduI GDGCHC 3 cut(s) 550, 804, 1259
SfaNI GCATC 2 cut(s) 730, 907
SfcI CTRYAG 1 cut(s) 1086
SinI GGWCC 3 cut(s) 67, 958, 1004
SmiMI CAYNNNNRTG 1 cut(s) 915
SpeI ACTAGT 1 cut(s) 1129
Sse9I AATT 2 cut(s) 430, 993
SsiI CCGC 7 cut(s) 130, 159, 305, 408, 502, 659, 1182
SspMI CTAG 2 cut(s) 948, 1130
SstI GAGCTC 2 cut(s) 804, 1259
StyD4I CCNGG 2 cut(s) 69, 583
StyI CCWWGG 1 cut(s) 1190
TaaI ACNGT 3 cut(s) 703, 886, 1090
TaqI TCGA 2 cut(s) 177, 232
TaqII GACCGA 1 cut(s) 192
TasI AATT 2 cut(s) 430, 993
TatI WGTACW 1 cut(s) 590
TauI GCSGC 2 cut(s) 132, 161
TfiI GAWTC 2 cut(s) 229, 329
Tru1I TTAA 4 cut(s) 251, 563, 839, 1146
Tru9I TTAA 4 cut(s) 251, 563, 839, 1146
TscAI CASTG 2 cut(s) 802, 1222
TspDTI ATGAA 4 cut(s) 281, 515, 741, 781
TspGWI ACGGA 2 cut(s) 237, 354
TspRI CASTG 2 cut(s) 802, 1222
VpaK11BI GGWCC 3 cut(s) 67, 958, 1004
VspI ATTAAT 1 cut(s) 839
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XspI CTAG 2 cut(s) 948, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.