Prupe.6G253500_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001131
Physical Location & Seq
Forward (+)
24712705 .. 24715980
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G253500.1

Sequence Viewer

Length: 1332 bp
ATGGGTCAGGGACTGAGTTGCAGAGAGACCCCTCAAAGTGGACTATTTGGACCTCTCCAGGATGGAGAATTGGAGCTCGTTGAGGCTATGCTGGAAGCAGACCCAAGTGTTTTTCAGCAGAGGAAAGGTCGTGCGAGGTTGTCTGCGTTGCATGTAGCTGCTGTTGAGGGTCAGATCGAGGTTCTCTCTCTGCTTTTGGATCGGTCTGTTATCAATCCGGATGTGGTAGATCGACATAAACTGACCCCGTTGATGTTGGCTGCAATAAACGGGAGGATCGCTTGCGTCCAAAAGCTTATCCAAGCTGGAGCAAATATATTGATGTTTGATTCCCTGAATGGAAGAACCTGCCTGCATTATGCTGCCTACTATGGTCATGCAGACTGCCTCCAAGCCATTCTTTCTGCTGCCCATTCCTCCCCTGTAGCAAGTTCTTGGGGATTTTCAAGATTTGTGAACATAAGAGATGCAGGTGGTGCAACCCCATTGCATTTAGCAGCCCGCCAAAGTCGGCCAGAGTGCGTTCATGCTCTTTTAGACAATGGGGCTCTTGTTTGTGCTTCAACTGGTGGATATGGCTACCCTGGAAGCACACCGCTTCACTTAGCTGCACGTGGTGGATCTTTAGATTGTGTCCGAGAGTTACTTGCTTGGGGTGCAGATCGGCTTCAATTAGATGCATCTGGGAGATTACCGTATACAGTTGCTCTGAGGCGCAAGCATCGAGCTTGCGCAGCTGTACTCAACCCTTCATCAGCGGAGCCTCTTGTTTGGCCATCACCTTTGAAGTTCATCAGCGAGCTTAATCCAGAGGCTAAAGCTTTGTTAGAAAGGGCGTTAACAGAAGCAAACATGGAGAGGGAGAAAGCTATCTTGAAGGAGGCAGTAAACTCACTTCCATCTCCTTTGCATTCAGATGCAGAGACTGATGATAATGCCTCTGAGGCTAGCGATGTGGATCTATGCTGCATATGCTTTGACCAGTTATGCACAATTGAGGTCGGACCATGCTGTCATCAAATGTGTGCACATTGTACCCTTGCCCTATGCTGCCACAAGAAGCCCGATCCTTCAACTACTTGTCCTACAGTCCCAGTTTGCCCCTTTTGCCGAACCACCATTACCCAACTGGTTGTGGCCAATATTAAGGCCAATAATGATGTGGAGCTGGAGATGAGTCCATCAAAGCCGAGGAGATCAAGAAAGTCTAATGTCAGTGAAGGTAGCAGTAGCTTCAAGGGGTTGTCTCCTATGAGCTCATTTGGAAGGATGGGCGGTCGTAGCTCAGGAAAGATAGCCGCTGAAACCAATGAGGAGATCCATAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

444

Amino Acids

47.49

Weight (kDa)

6.53

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
AasI GACNNNNNNGTC 1 cut(s) 1011
Acc16I TGCGCA 1 cut(s) 733
Acc36I ACCTGC 2 cut(s) 356, 461
AccI GTMKAC 1 cut(s) 698
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 5 cut(s) 502, 596, 758, 1275, 1299
AclWI GGATC 6 cut(s) 207, 284, 628, 966, 1061, 1312
AcoI YGGCCR 3 cut(s) 512, 773, 1137
AcvI CACGTG 1 cut(s) 614
AdeI CACNNNGTG 1 cut(s) 617
AfaI GTAC 2 cut(s) 741, 1036
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 7 cut(s) 447, 564, 671, 787, 877, 1074, 1237
AjnI CCWGG 2 cut(s) 57, 583
Alw21I GWGCWC 3 cut(s) 78, 1030, 1259
Alw26I GTCTC 3 cut(s) 20, 917, 1251
Alw44I GTGCAC 1 cut(s) 1026
AlwI GGATC 6 cut(s) 207, 284, 628, 966, 1061, 1312
AlwNI CAGNNNCTG 2 cut(s) 13, 926
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 4 cut(s) 512, 773, 1137, 1149
ApaLI GTGCAC 1 cut(s) 1026
ApeKI GCWGC 9 cut(s) 158, 260, 362, 407, 497, 608, 734, 966, 1050
AspLEI GCGC 2 cut(s) 717, 734
AspS9I GGNCC 2 cut(s) 50, 1004
AsuHPI GGTGA 1 cut(s) 771
AsuNHI GCTAGC 1 cut(s) 947
AvaII GGWCC 2 cut(s) 50, 1004
BaeGI GKGCMC 1 cut(s) 1030
BalI TGGCCA 2 cut(s) 775, 1139
BanII GRGCYC 3 cut(s) 78, 550, 1259
BarI GAAGNNNNNNTAC 2 cut(s) 879, 911
BbrPI CACGTG 1 cut(s) 614
Bbv12I GWGCWC 3 cut(s) 78, 1030, 1259
BbvI GCAGC 9 cut(s) 145, 247, 349, 394, 509, 595, 746, 953, 1037
BccI CCATC 5 cut(s) 56, 784, 907, 1189, 1264
BciT130I CCWGG 2 cut(s) 59, 585
BcoDI GTCTC 3 cut(s) 20, 917, 1251
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 2 cut(s) 423, 1086
BfuAI ACCTGC 2 cut(s) 356, 461
BglI GCCNNNNNGGC 1 cut(s) 944
Bme1390I CCNGG 2 cut(s) 59, 585
Bme18I GGWCC 2 cut(s) 50, 1004
BmgT120I GGNCC 2 cut(s) 50, 1004
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 2 cut(s) 59, 585
BmrI ACTGGG 1 cut(s) 1088
BmsI GCATC 5 cut(s) 457, 667, 689, 730, 907
BmtI GCTAGC 1 cut(s) 951
BmuI ACTGGG 1 cut(s) 1088
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 3 cut(s) 41, 327, 1190
Bpu10I CCTNAGC 1 cut(s) 1285
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 583, 1190
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse1I ACTGG 4 cut(s) 571, 982, 1094, 1134
Bse3DI GCAATG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 957
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 2 cut(s) 59, 585
BseDI CCNNGG 2 cut(s) 583, 1190
BseGI GGATG 3 cut(s) 67, 226, 1275
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 4 cut(s) 5, 701, 933, 1299
BseNI ACTGG 4 cut(s) 571, 982, 1094, 1134
BseRI GAGGAG 2 cut(s) 1207, 1328
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 9 cut(s) 145, 247, 349, 394, 509, 595, 746, 953, 1037
BsgI GTGCAG 2 cut(s) 594, 678
Bsh1285I CGRYCG 1 cut(s) 1279
BshFI GGCC 4 cut(s) 514, 775, 1139, 1151
BsiEI CGRYCG 1 cut(s) 1279
BsiHKAI GWGCWC 3 cut(s) 78, 1030, 1259
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 24, 1076
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 3 cut(s) 20, 917, 1251
BsmFI GGGAC 2 cut(s) 24, 1076
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 4 cut(s) 514, 775, 1139, 1151
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 4 cut(s) 78, 550, 1030, 1259
Bsp13I TCCGGA 1 cut(s) 217
BspACI CCGC 5 cut(s) 502, 596, 758, 1275, 1299
BspANI GGCC 4 cut(s) 514, 775, 1139, 1151
BspCNI CTCAG 4 cut(s) 6, 702, 934, 1298
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 762
BspMI ACCTGC 2 cut(s) 356, 461
BspOI GCTAGC 1 cut(s) 951
BspPI GGATC 6 cut(s) 207, 284, 628, 966, 1061, 1312
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 485
BsrI ACTGG 4 cut(s) 571, 982, 1094, 1134
BssECI CCNNGG 2 cut(s) 583, 1190
BssNAI GTATAC 1 cut(s) 699
Bst1107I GTATAC 1 cut(s) 699
Bst2UI CCWGG 2 cut(s) 59, 585
Bst4CI ACNGT 3 cut(s) 696, 703, 1090
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 7 cut(s) 283, 353, 502, 719, 730, 800, 949
BstDEI CTNAG 5 cut(s) 14, 604, 710, 942, 1285
BstF5I GGATG 3 cut(s) 67, 226, 1275
BstHHI GCGC 2 cut(s) 717, 734
BstMAI GTCTC 3 cut(s) 20, 917, 1251
BstMCI CGRYCG 1 cut(s) 1279
BstMWI GCNNNNNNNGC 7 cut(s) 476, 656, 734, 944, 972, 1107, 1281
BstNI CCWGG 2 cut(s) 59, 585
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 57, 583
BstSFI CTRYAG 2 cut(s) 423, 1086
BstSLI GKGCMC 1 cut(s) 1030
BstV1I GCAGC 9 cut(s) 145, 247, 349, 394, 509, 595, 746, 953, 1037
BstX2I RGATCY 3 cut(s) 620, 958, 1317
BstYI RGATCY 3 cut(s) 620, 958, 1317
BstZ17I GTATAC 1 cut(s) 699
BsuRI GGCC 4 cut(s) 514, 775, 1139, 1151
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 3 cut(s) 67, 226, 1275
BtsIMutI CAGTG 1 cut(s) 1222
BveI ACCTGC 2 cut(s) 356, 461
Cac8I GCNNGC 7 cut(s) 283, 353, 502, 719, 730, 800, 949
CaiI CAGNNNCTG 2 cut(s) 13, 926
CfoI GCGC 2 cut(s) 717, 734
Cfr13I GGNCC 2 cut(s) 50, 1004
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 740, 1035
CviAII CATG 5 cut(s) 152, 377, 527, 853, 1008
CviQI GTAC 2 cut(s) 740, 1035
DdeI CTNAG 5 cut(s) 14, 604, 710, 942, 1285
DraIII CACNNNGTG 1 cut(s) 617
DrdI GACNNNNNNGTC 1 cut(s) 1011
DseDI GACNNNNNNGTC 1 cut(s) 1011
EaeI YGGCCR 3 cut(s) 512, 773, 1137
Ecl136II GAGCTC 2 cut(s) 76, 1257
Eco24I GRGCYC 3 cut(s) 78, 550, 1259
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 50, 1004
Eco53kI GAGCTC 2 cut(s) 76, 1257
Eco72I CACGTG 1 cut(s) 614
EcoICRI GAGCTC 2 cut(s) 76, 1257
EcoRII CCWGG 2 cut(s) 57, 583
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 3 cut(s) 78, 550, 1259
FaeI CATG 5 cut(s) 155, 380, 530, 856, 1011
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 2 cut(s) 24, 1076
FatI CATG 5 cut(s) 151, 376, 526, 852, 1007
FauI CCCGC 1 cut(s) 509
FauNDI CATATG 1 cut(s) 971
FblI GTMKAC 1 cut(s) 698
FokI GGATG 3 cut(s) 74, 233, 1282
FriOI GRGCYC 3 cut(s) 78, 550, 1259
FspBI CTAG 1 cut(s) 948
FspI TGCGCA 1 cut(s) 733
GlaI GCGC 2 cut(s) 716, 733
GsuI CTGGAG 3 cut(s) 41, 327, 1190
HaeIII GGCC 4 cut(s) 514, 775, 1139, 1151
HapII CCGG 1 cut(s) 218
HgaI GACGC 1 cut(s) 274
HhaI GCGC 2 cut(s) 717, 734
Hin1II CATG 5 cut(s) 155, 380, 530, 856, 1011
Hin6I GCGC 2 cut(s) 715, 732
HinP1I GCGC 2 cut(s) 715, 732
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HindIII AAGCTT 2 cut(s) 293, 819
HinfI GANTC 2 cut(s) 329, 1177
HpaI GTTAAC 1 cut(s) 840
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 6 cut(s) 41, 457, 699, 840, 889, 1028
Hpy188I TCNGA 6 cut(s) 174, 638, 711, 916, 943, 1004
Hpy188III TCNNGA 6 cut(s) 218, 447, 809, 874, 1200, 1287
Hpy8I GTNNAC 6 cut(s) 41, 457, 699, 840, 889, 1028
HpyAV CCTTC 5 cut(s) 759, 871, 1080, 1214, 1260
HpyCH4III ACNGT 3 cut(s) 696, 703, 1090
HpyCH4IV ACGT 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 7 cut(s) 476, 656, 734, 944, 972, 1107, 1281
HpyF3I CTNAG 5 cut(s) 14, 604, 710, 942, 1285
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 5 cut(s) 155, 380, 530, 856, 1011
HspAI GCGC 2 cut(s) 715, 732
Kpn2I TCCGGA 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 840
LmnI GCTCC 4 cut(s) 73, 308, 760, 1165
Lsp1109I GCAGC 9 cut(s) 145, 247, 349, 394, 509, 595, 746, 953, 1037
LweI GCATC 5 cut(s) 457, 667, 689, 730, 907
MaeI CTAG 1 cut(s) 948
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 1 cut(s) 642
MboII GAAGA 1 cut(s) 354
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 3 cut(s) 620, 958, 1317
MhlI GDGCHC 4 cut(s) 78, 550, 1030, 1259
MlsI TGGCCA 2 cut(s) 775, 1139
MluCI AATT 3 cut(s) 68, 671, 993
MluNI TGGCCA 2 cut(s) 775, 1139
MlyI GAGTC 1 cut(s) 1186
MmeI TCCRAC 1 cut(s) 982
Mox20I TGGCCA 2 cut(s) 775, 1139
Mph1103I ATGCAT 1 cut(s) 682
MroI TCCGGA 1 cut(s) 217
MscI TGGCCA 2 cut(s) 775, 1139
MseI TTAA 3 cut(s) 804, 839, 1146
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 2 cut(s) 775, 1139
MspA1I CMGCKG 3 cut(s) 737, 758, 1301
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 59, 585
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 2 cut(s) 59, 585
MwoI GCNNNNNNNGC 7 cut(s) 476, 656, 734, 944, 972, 1107, 1281
NdeI CATATG 1 cut(s) 971
NheI GCTAGC 1 cut(s) 947
NlaIII CATG 5 cut(s) 155, 380, 530, 856, 1011
NlaIV GGNNCC 1 cut(s) 762
NmeAIII GCCGAG 1 cut(s) 1215
NsbI TGCGCA 1 cut(s) 733
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 155
PaqCI CACCTGC 1 cut(s) 461
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 1 cut(s) 329
PfoI TCCNGGA 1 cut(s) 57
PleI GAGTC 1 cut(s) 1185
PmaCI CACGTG 1 cut(s) 614
PmlI CACGTG 1 cut(s) 614
PpsI GAGTC 1 cut(s) 1185
Ppu21I YACGTR 1 cut(s) 614
Psp124BI GAGCTC 2 cut(s) 78, 1259
Psp6I CCWGG 2 cut(s) 57, 583
PspCI CACGTG 1 cut(s) 614
PspGI CCWGG 2 cut(s) 57, 583
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 50, 1004
PsrI GAACNNNNNNTAC 2 cut(s) 1105, 1137
PstNI CAGNNNCTG 2 cut(s) 13, 926
PsuI RGATCY 3 cut(s) 620, 958, 1317
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 2 cut(s) 741, 1036
RsaNI GTAC 2 cut(s) 740, 1035
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 2 cut(s) 78, 1259
SaqAI TTAA 3 cut(s) 804, 839, 1146
Sau96I GGNCC 2 cut(s) 50, 1004
SchI GAGTC 1 cut(s) 1186
ScrFI CCNGG 2 cut(s) 59, 585
SduI GDGCHC 4 cut(s) 78, 550, 1030, 1259
SfaNI GCATC 5 cut(s) 457, 667, 689, 730, 907
SfcI CTRYAG 2 cut(s) 423, 1086
SinI GGWCC 2 cut(s) 50, 1004
SmiMI CAYNNNNRTG 1 cut(s) 915
Sse9I AATT 3 cut(s) 68, 671, 993
SsiI CCGC 5 cut(s) 502, 596, 758, 1275, 1299
SspI AATATT 1 cut(s) 1144
SspMI CTAG 1 cut(s) 948
SstI GAGCTC 2 cut(s) 78, 1259
StyD4I CCNGG 2 cut(s) 57, 583
TaaI ACNGT 3 cut(s) 696, 703, 1090
TaiI ACGT 1 cut(s) 616
TaqI TCGA 3 cut(s) 177, 232, 724
TaqII GACCGA 1 cut(s) 192
TasI AATT 3 cut(s) 68, 671, 993
TatI WGTACW 1 cut(s) 739
TauI GCSGC 1 cut(s) 1301
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 3 cut(s) 804, 839, 1146
Tru9I TTAA 3 cut(s) 804, 839, 1146
TscAI CASTG 1 cut(s) 1222
TseI GCWGC 9 cut(s) 158, 260, 362, 407, 497, 608, 734, 966, 1050
TspDTI ATGAA 3 cut(s) 515, 741, 781
TspRI CASTG 1 cut(s) 1222
VneI GTGCAC 1 cut(s) 1026
VpaK11BI GGWCC 2 cut(s) 50, 1004
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 2 cut(s) 1126, 1159
XmiI GTMKAC 1 cut(s) 698
XspI CTAG 1 cut(s) 948
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.