pycom04g11370

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001131
Physical Location & Seq
Forward (+)
14259659 .. 14262261
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11370.2

Sequence Viewer

Length: 1332 bp
ATGGGTCAGGGCCTGAGCTGCGGAGAGCCTCGTGAGAATGGAGTCTTTGGAGCTGTGGAGAATGGGGACTTGGGGCTTGTCGGAGCCATGGTGGAAGCTGACCCAAGTGTTTTGGAGCTGAGCAAAGGCCGCCGCCGGCTGTCTGCTCTGCATGTCGCCGCAGCAAATGGCCGGATCGAGGTTCTGTCTATGCTGTTGGATCGGTCTGTTATCAATCCGGATGTGGTGAATCGATACAAACAGACTCCGTTAATGTTGGCTGCGATGAATGGGAATATCACCTGTGTCCAAAAGCTTATCCAAGCAGGAGCAAATATTTTGATGTTTGATTCCCTGAATGGAAGAACTTGTCTGCATTACGCTGCATACTATGGCCATTCAGACTGCCTTCAAGCCATTCTTTCCGCTGCACATTCCACCCCTGTTGCAAATTCTTGGGGATTTGCGAGATTTGTGAACATAAGAGATGCAGGTGGTGCAACCCCATTGCATTTAGCAGCCCGCCAAAGACGGCCAGAGTGCGTTCATGTTCTTTTAAACAATGGAGCTCTTGTTTGTGCTTCAACTGGTGGATATGGCTACCCTGGAAGCACACCACTTCATTTAGCTGCTCGTGGTGGATCTTTAGATTGTGTCCGAGAGTTGCTTGCTTGGGGAGCGGATCGGCTTCAGTTAGACTCTTCTGGGAGAATACCATATACAGTTGCTCTGAAGCACAAGCATCAAGCATGTGCTGCCGTACTGAACCCTTCATCAGCAGAGCCTCTGGTCTGGCCATCACCTTTGAAGTTCATCAGTGAGCTCAATCCAGAGGCTAAAGCTTTGTTAGAAAAGGCATTAACGGAAGCAAACATGGAGAGGGAGAAAGCAATCTTGAAGGAGACAGTAGCCTCACTCCCATCTCCTCTGCATTCAGATACTGAGGCTGATGATAATGCCTCTGAGGCTAGTGATGTGGACCTATGCTGCATATGCTTTGACCAGTTATGCACAATTGAGGTCGTACCATGTGGTCATCAAATGTGTGCTCATTGCACCCTAGCCCTATGCTGCCACAAGAAGCCCGATCGATCCTCTACTTGTCCTCCAGTCCCCGTTTGCCCCTTTTGCCGAACCACCATTACCCTACTAGTTGTTGCCAACATTAAGGCCAGCGATGACATGGAGCCGGAAATGAGTCCCGCAAAGCCAAGGAGATCAAGAAAGTCTAATGTCAGCGAAGGCAGCAGTAGCTTCAAGGTGTTGTCGCCTATGAGCTCGTTTGGAAGGATGGGCAATCGCAGTTCAGGAAAGGTAGCTGCTGAATGCCATGAGGAGATCCATAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

444

Amino Acids

47.4

Weight (kDa)

6.92

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 659
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 8 cut(s) 21, 130, 133, 159, 405, 502, 659, 1182
AclWI GGATC 6 cut(s) 182, 207, 628, 669, 1065, 1312
AcoI YGGCCR 4 cut(s) 169, 373, 512, 773
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 2 cut(s) 653, 731
AfaI GTAC 2 cut(s) 741, 1005
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 5 cut(s) 392, 564, 787, 877, 1237
AhlI ACTAGT 1 cut(s) 1129
AjnI CCWGG 1 cut(s) 583
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
Alw21I GWGCWC 4 cut(s) 550, 804, 1030, 1259
Alw26I GTCTC 1 cut(s) 875
AlwI GGATC 6 cut(s) 182, 207, 628, 669, 1065, 1312
AlwNI CAGNNNCTG 2 cut(s) 13, 920
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 7 cut(s) 10, 127, 169, 373, 512, 773, 1149
ApoI RAATTY 1 cut(s) 430
AspS9I GGNCC 2 cut(s) 10, 958
AsuHPI GGTGA 3 cut(s) 238, 271, 771
AvaII GGWCC 1 cut(s) 958
BalI TGGCCA 2 cut(s) 375, 775
BanII GRGCYC 3 cut(s) 550, 804, 1259
BauI CACGAG 2 cut(s) 30, 612
Bbv12I GWGCWC 4 cut(s) 550, 804, 1030, 1259
BccI CCATC 3 cut(s) 784, 907, 1264
BceAI ACGGC 2 cut(s) 527, 722
BciT130I CCWGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 875
BcuI ACTAGT 1 cut(s) 1129
BfaI CTAG 3 cut(s) 948, 1040, 1130
BfuAI ACCTGC 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 944
BlpI GCTNAGC 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 585
Bme18I GGWCC 1 cut(s) 958
BmgT120I GGNCC 2 cut(s) 10, 958
BmiI GGNNCC 2 cut(s) 85, 1167
BmrFI CCNGG 1 cut(s) 585
BmsI GCATC 2 cut(s) 457, 730
BpmI CTGGAG 1 cut(s) 1071
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 119
Bsa29I ATCGAT 2 cut(s) 232, 1069
BsaJI CCNNGG 3 cut(s) 87, 583, 1190
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 6 cut(s) 75, 105, 537, 567, 1069, 1099
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 3 cut(s) 571, 982, 1088
Bse3DI GCAATG 2 cut(s) 485, 1030
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 1 cut(s) 585
BseCI ATCGAT 2 cut(s) 232, 1069
BseDI CCNNGG 3 cut(s) 87, 583, 1190
BseGI GGATG 2 cut(s) 226, 1275
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 485, 1030
BseMII CTCAG 4 cut(s) 5, 110, 912, 933
BseNI ACTGG 3 cut(s) 571, 982, 1088
BseRI GAGGAG 2 cut(s) 894, 1328
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 1069
BshFI GGCC 7 cut(s) 12, 129, 171, 375, 514, 775, 1151
BshVI ATCGAT 2 cut(s) 232, 1069
BsiEI CGRYCG 1 cut(s) 1069
BsiHKAI GWGCWC 4 cut(s) 550, 804, 1030, 1259
BsiSI CCGG 4 cut(s) 136, 172, 218, 1169
BslFI GGGAC 3 cut(s) 80, 1076, 1164
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 875
BsmFI GGGAC 3 cut(s) 80, 1076, 1164
BsmI GAATGC 2 cut(s) 910, 1310
BsnI GGCC 7 cut(s) 12, 129, 171, 375, 514, 775, 1151
Bsp1286I GDGCHC 4 cut(s) 550, 804, 1030, 1259
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 8 cut(s) 21, 130, 133, 159, 405, 502, 659, 1182
BspANI GGCC 7 cut(s) 12, 129, 171, 375, 514, 775, 1151
BspCNI CTCAG 4 cut(s) 6, 111, 913, 934
BspDI ATCGAT 2 cut(s) 232, 1069
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 85, 1167
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 6 cut(s) 182, 207, 628, 669, 1065, 1312
BsrBI CCGCTC 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 485, 1030
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 3 cut(s) 571, 982, 1088
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 3 cut(s) 87, 583, 1190
BssMI GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
BssSI CACGAG 2 cut(s) 30, 612
BssT1I CCWWGG 2 cut(s) 87, 1190
Bst2BI CACGAG 2 cut(s) 30, 612
Bst2UI CCWGG 1 cut(s) 585
Bst4CI ACNGT 2 cut(s) 703, 886
Bst6I CTCTTC 1 cut(s) 685
BstAPI GCANNNNNTGC 2 cut(s) 476, 734
BstC8I GCNNGC 4 cut(s) 137, 502, 648, 1153
BstDEI CTNAG 4 cut(s) 14, 119, 921, 942
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 226, 1275
BstKTI GATC 8 cut(s) 177, 202, 623, 664, 1069, 1073, 1199, 1320
BstMAI GTCTC 1 cut(s) 875
BstMBI GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
BstMCI CGRYCG 1 cut(s) 1069
BstNI CCWGG 1 cut(s) 585
BstNSI RCATGY 2 cut(s) 155, 732
BstSCI CCNGG 1 cut(s) 583
BstX2I RGATCY 2 cut(s) 620, 1317
BstYI RGATCY 2 cut(s) 620, 1317
Bsu15I ATCGAT 2 cut(s) 232, 1069
BsuRI GGCC 7 cut(s) 12, 129, 171, 375, 514, 775, 1151
BsuTUI ATCGAT 2 cut(s) 232, 1069
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 2 cut(s) 278, 1170
BtsCI GGATG 2 cut(s) 226, 1275
BtsIMutI CAGTG 1 cut(s) 802
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 4 cut(s) 137, 502, 648, 1153
CaiI CAGNNNCTG 2 cut(s) 13, 920
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 10, 958
ClaI ATCGAT 2 cut(s) 232, 1069
Csp6I GTAC 2 cut(s) 740, 1004
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 8 cut(s) 88, 152, 527, 729, 853, 1008, 1162, 1310
CviQI GTAC 2 cut(s) 740, 1004
DdeI CTNAG 4 cut(s) 14, 119, 921, 942
DpnI GATC 8 cut(s) 176, 201, 622, 663, 1068, 1072, 1198, 1319
DpnII GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
DraI TTTAAA 1 cut(s) 537
EaeI YGGCCR 4 cut(s) 169, 373, 512, 773
Eam1104I CTCTTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 685
Ecl136II GAGCTC 3 cut(s) 548, 802, 1257
Eco130I CCWWGG 2 cut(s) 87, 1190
Eco24I GRGCYC 3 cut(s) 550, 804, 1259
Eco47I GGWCC 1 cut(s) 958
Eco53kI GAGCTC 3 cut(s) 548, 802, 1257
Eco57I CTGAAG 2 cut(s) 653, 731
EcoICRI GAGCTC 3 cut(s) 548, 802, 1257
EcoO109I RGGNCCY 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 87, 1190
EcoT38I GRGCYC 3 cut(s) 550, 804, 1259
ErhI CCWWGG 2 cut(s) 87, 1190
FaeI CATG 8 cut(s) 91, 155, 530, 732, 856, 1011, 1165, 1313
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 3 cut(s) 80, 1076, 1164
FatI CATG 8 cut(s) 87, 151, 526, 728, 852, 1007, 1161, 1309
FauI CCCGC 2 cut(s) 509, 1189
FauNDI CATATG 1 cut(s) 971
FokI GGATG 2 cut(s) 233, 1282
FriOI GRGCYC 3 cut(s) 550, 804, 1259
FspBI CTAG 3 cut(s) 948, 1040, 1130
GsuI CTGGAG 1 cut(s) 1071
HaeIII GGCC 7 cut(s) 12, 129, 171, 375, 514, 775, 1151
HapII CCGG 4 cut(s) 136, 172, 218, 1169
Hin1II CATG 8 cut(s) 91, 155, 530, 732, 856, 1011, 1165, 1313
HindIII AAGCTT 2 cut(s) 293, 819
HinfI GANTC 6 cut(s) 42, 229, 244, 329, 677, 1177
HpaII CCGG 4 cut(s) 136, 172, 218, 1169
HphI GGTGA 3 cut(s) 238, 271, 771
Hpy166II GTNNAC 2 cut(s) 457, 958
Hpy188I TCNGA 6 cut(s) 83, 382, 638, 711, 916, 943
Hpy188III TCNNGA 6 cut(s) 32, 218, 809, 874, 1200, 1287
Hpy8I GTNNAC 2 cut(s) 457, 958
HpyAV CCTTC 5 cut(s) 398, 759, 871, 1214, 1260
HpyCH4III ACNGT 2 cut(s) 703, 886
HpyF3I CTNAG 4 cut(s) 14, 119, 921, 942
Hsp92II CATG 8 cut(s) 91, 155, 530, 732, 856, 1011, 1165, 1313
Kpn2I TCCGGA 1 cut(s) 217
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
LmnI GCTCC 7 cut(s) 50, 83, 115, 308, 545, 656, 1165
LweI GCATC 2 cut(s) 457, 730
MaeI CTAG 3 cut(s) 948, 1040, 1130
MalI GATC 8 cut(s) 176, 201, 622, 663, 1068, 1072, 1198, 1319
MbiI CCGCTC 1 cut(s) 659
MboI GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
MboII GAAGA 2 cut(s) 354, 672
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 2 cut(s) 620, 1317
MhlI GDGCHC 4 cut(s) 550, 804, 1030, 1259
MlsI TGGCCA 2 cut(s) 375, 775
MluCI AATT 2 cut(s) 430, 993
MluNI TGGCCA 2 cut(s) 375, 775
MlyI GAGTC 4 cut(s) 51, 238, 671, 1186
MmeI TCCRAC 2 cut(s) 61, 177
Mox20I TGGCCA 2 cut(s) 375, 775
MroI TCCGGA 1 cut(s) 217
MroNI GCCGGC 1 cut(s) 135
MscI TGGCCA 2 cut(s) 375, 775
MseI TTAA 4 cut(s) 251, 536, 839, 1146
Msp20I TGGCCA 2 cut(s) 375, 775
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 4 cut(s) 136, 172, 218, 1169
MspR9I CCNGG 1 cut(s) 585
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 2 cut(s) 910, 1310
MvaI CCWGG 1 cut(s) 585
NaeI GCCGGC 1 cut(s) 137
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 971
NdeII GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 8 cut(s) 91, 155, 530, 732, 856, 1011, 1165, 1313
NlaIV GGNNCC 2 cut(s) 85, 1167
NspI RCATGY 2 cut(s) 155, 732
PaqCI CACCTGC 1 cut(s) 461
PctI GAATGC 2 cut(s) 910, 1310
PdiI GCCGGC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 229, 329
Ple19I CGATCG 1 cut(s) 1069
PleI GAGTC 4 cut(s) 50, 238, 671, 1185
PpsI GAGTC 4 cut(s) 50, 238, 671, 1185
Psp124BI GAGCTC 3 cut(s) 550, 804, 1259
Psp6I CCWGG 1 cut(s) 583
PspGI CCWGG 1 cut(s) 583
PspN4I GGNNCC 2 cut(s) 85, 1167
PspPI GGNCC 2 cut(s) 10, 958
PsrI GAACNNNNNNTAC 2 cut(s) 1105, 1137
PstNI CAGNNNCTG 2 cut(s) 13, 920
PsuI RGATCY 2 cut(s) 620, 1317
PvuI CGATCG 1 cut(s) 1069
RsaI GTAC 2 cut(s) 741, 1005
RsaNI GTAC 2 cut(s) 740, 1004
SacI GAGCTC 3 cut(s) 550, 804, 1259
SaqAI TTAA 4 cut(s) 251, 536, 839, 1146
Sau3AI GATC 8 cut(s) 174, 199, 620, 661, 1066, 1070, 1196, 1317
Sau96I GGNCC 2 cut(s) 10, 958
SchI GAGTC 4 cut(s) 51, 238, 671, 1186
ScrFI CCNGG 1 cut(s) 585
SduI GDGCHC 4 cut(s) 550, 804, 1030, 1259
SfaNI GCATC 2 cut(s) 457, 730
SinI GGWCC 1 cut(s) 958
SpeI ACTAGT 1 cut(s) 1129
Sse9I AATT 2 cut(s) 430, 993
SsiI CCGC 8 cut(s) 21, 130, 133, 159, 405, 502, 659, 1182
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 948, 1040, 1130
SstI GAGCTC 3 cut(s) 550, 804, 1259
StyD4I CCNGG 1 cut(s) 583
StyI CCWWGG 2 cut(s) 87, 1190
TaaI ACNGT 2 cut(s) 703, 886
TaqI TCGA 3 cut(s) 177, 232, 1069
TaqII GACCGA 1 cut(s) 192
TasI AATT 2 cut(s) 430, 993
TauI GCSGC 3 cut(s) 132, 135, 161
TfiI GAWTC 2 cut(s) 229, 329
Tru1I TTAA 4 cut(s) 251, 536, 839, 1146
Tru9I TTAA 4 cut(s) 251, 536, 839, 1146
TscAI CASTG 1 cut(s) 802
TspDTI ATGAA 5 cut(s) 281, 515, 590, 741, 781
TspGWI ACGGA 2 cut(s) 237, 857
TspRI CASTG 1 cut(s) 802
VpaK11BI GGWCC 1 cut(s) 958
XapI RAATTY 1 cut(s) 430
XceI RCATGY 2 cut(s) 155, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XspI CTAG 3 cut(s) 948, 1040, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.