RchiOBHm_Chr3g0466811

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001131
Physical Location & Seq
Reverse (-)
13172468 .. 13176014
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43284

Sequence Viewer

Length: 1326 bp
ATGGGTCAGGGGCTGAGTTGCGGAGAGCCTCGGGAGATTGGTCTGTTTGGGGCCGTGGAAAATGGGGACTTGGAGGGGGTTCAGGATATGGTGGAAGCTGACCCCAGTGTTTTGGACCAGAGAAAAGGCCGTGGCAAGCTTTCTGCTATGCATGTAGCTGCTGCTGGTGGTCAGATCGAGGTTCTTTCCATGCTTTTGGATCGGTCTGTTAGTCCGGATGTAGTGAATCGCCATAAGCAGACTCCGCTGATGTTGGCTGCAATGCATGGGAATATCGCATGCGTTAGAAAGCTAATCCAAGCCGGAGCAAATATATTGATGTTCGATTCCTTAAATGGAAGAACTTGCCTGCATTATGCTGCATATTATGGCCATTCAGACTGCCTCCAAGCCTTTTTATCCGCCGCACAATCCACCACTGTTGCAAATTCTTGGGGATTTGCAAGATTTGTGAATATTAGAGATGGAGGTGGAGCAACCCCATTGCACTTGGCTGCCCGTCAAAAACGGGCCGAGTGTGTGCATGTTCTTTTGGATAGCGGGGCTCTTGTTTGTGCTTCAACTGGTGGATACAGCTACCCTGGAAGCACACCACTTCATTTAGCAGCTCGTGCTGGTTCTTTAGATTGCGTCAGAGAGTTGCTTGCTTGGGGAGCGGATCGGCTTCAATTAGACTCATCAGGGAGAATCCCATACCTGGTTGCTCTGAAGCACAAGCACAGAGCATGTGCCGCGATACTGAACCCTTCATCAGCAGAGCCTCTTGTCTGGCCATCACCTTTGAAGTTTATCAGCGAACTTAATCCAGAGGCTAAAGCTTTGTTGGAGAAGGCTTTAATGGAATCAAACATGGAGAGGGAGAAAGCTATCTTGAAGGAGATAGTCTCCATTCCATCTCCTTTGCATTCAGATGTAGAGGGTGATGATAATGCCTCTGAGGCTACCGATGTGGATCTATGCTGCATATGCTTTGACCAGTTATGCACAATTGAGGTTGGACCATGTGGTCATCAAATGTGTGCACATTGCACCCTCGCCCTCTGCTGCCACAAGAAGCCCAACCCCTCATCTCTATCTTGCCCTACAGTCCCTGTTTGCCCCTTTTGTCGGACCAGCATCACCCAACTAGTTGTTGCCAATATCAAGGCCAACAATGATGTGGAGGTGGAAATGAGTCCCTCAAAACCAAGGAAATCAAGAAAATCTAATGTCAGTGAAGGCAGCAGCAGCTTCAAGGGCCTGTCGCCTTTGGGCTCATTTGGAAGATTGGGTGGTCGCAATTCAGGAAGGGTATCTGCTGAATGCAATGAAGACATCAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

441

Amino Acids

47.02

Weight (kDa)

6.69

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 106 6.2e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 50 - 99 4.7e-08 Ankyrin repeats (many copies)
Ank PF00023 80 - 107 3.7e-06 Ankyrin repeat
Ank_4 PF13637 85 - 133 8.8e-11 Ankyrin repeats (many copies)
Ank_2 PF12796 112 - 183 4.6e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 195 - 248 1.2e-06 Ankyrin repeats (3 copies)
Ank PF00023 195 - 225 2.6e-07 Ankyrin repeat
RING_XB3-XBAT31 PF24921 319 - 375 4.4e-21 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1005
AccBSI CCGCTC 1 cut(s) 656
AccII CGCG 1 cut(s) 734
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 7 cut(s) 21, 245, 402, 405, 540, 656, 732
AclWI GGATC 3 cut(s) 207, 666, 960
AcoI YGGCCR 2 cut(s) 370, 770
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 728
AfiI CCNNNNNNNGG 2 cut(s) 697, 1107
AgsI TTSAA 5 cut(s) 561, 668, 784, 874, 1234
AhlI ACTAGT 1 cut(s) 1126
AjnI CCWGG 2 cut(s) 580, 696
AluBI AGCT 9 cut(s) 98, 139, 158, 292, 576, 608, 818, 866, 1230
AluI AGCT 9 cut(s) 98, 139, 158, 292, 576, 608, 818, 866, 1230
Alw21I GWGCWC 1 cut(s) 1024
Alw26I GTCTC 1 cut(s) 889
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 3 cut(s) 207, 666, 960
AlwNI CAGNNNCTG 2 cut(s) 13, 1091
Ama87I CYCGRG 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 7 cut(s) 51, 127, 370, 510, 770, 1146, 1237
ApaLI GTGCAC 1 cut(s) 1020
ApoI RAATTY 1 cut(s) 427
AspS9I GGNCC 6 cut(s) 51, 115, 510, 998, 1110, 1237
AsuHPI GGTGA 3 cut(s) 768, 932, 1111
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 3 cut(s) 115, 998, 1110
BaeGI GKGCMC 1 cut(s) 1024
BalI TGGCCA 2 cut(s) 372, 772
BanII GRGCYC 2 cut(s) 547, 1256
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 1317
Bbv12I GWGCWC 1 cut(s) 1024
BccI CCATC 3 cut(s) 458, 781, 901
BceAI ACGGC 2 cut(s) 38, 114
BciT130I CCWGG 2 cut(s) 582, 698
BciVI GTATCC 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 889
BcuI ACTAGT 1 cut(s) 1126
BfaI CTAG 1 cut(s) 1127
BfmI CTRYAG 1 cut(s) 1083
BfuI GTATCC 1 cut(s) 563
BglI GCCNNNNNGGC 1 cut(s) 938
Bme1390I CCNGG 2 cut(s) 582, 698
Bme18I GGWCC 3 cut(s) 115, 998, 1110
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 6 cut(s) 51, 115, 510, 998, 1110, 1237
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 2 cut(s) 582, 698
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 1125
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 1317
BplI GAGNNNNNCTC 2 cut(s) 869, 901
BsaBI GATNNNNATC 1 cut(s) 951
BsaJI CCNNGG 5 cut(s) 29, 54, 130, 580, 1187
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 697, 1107
Bse1I ACTGG 3 cut(s) 105, 568, 976
Bse3DI GCAATG 4 cut(s) 267, 482, 1024, 1312
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 2 cut(s) 582, 698
BseDI CCNNGG 5 cut(s) 29, 54, 130, 580, 1187
BseGI GGATG 1 cut(s) 223
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 2 cut(s) 697, 1107
BseMI GCAATG 4 cut(s) 267, 482, 1024, 1312
BseMII CTCAG 2 cut(s) 5, 927
BseNI ACTGG 3 cut(s) 105, 568, 976
BseSI GKGCMC 1 cut(s) 1024
Bsh1236I CGCG 1 cut(s) 734
BshFI GGCC 7 cut(s) 53, 129, 372, 512, 772, 1148, 1239
BsiHKAI GWGCWC 1 cut(s) 1024
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 2 cut(s) 215, 303
BslFI GGGAC 3 cut(s) 80, 1073, 1161
BslI CCNNNNNNNGG 2 cut(s) 697, 1107
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 3 cut(s) 80, 1073, 1161
BsmI GAATGC 2 cut(s) 904, 1307
BsnI GGCC 7 cut(s) 53, 129, 372, 512, 772, 1148, 1239
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 3 cut(s) 547, 1024, 1256
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 4 cut(s) 174, 199, 658, 952
BspACI CCGC 7 cut(s) 21, 245, 402, 405, 540, 656, 732
BspANI GGCC 7 cut(s) 53, 129, 372, 512, 772, 1148, 1239
BspCNI CTCAG 2 cut(s) 6, 928
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 1 cut(s) 734
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 3 cut(s) 207, 666, 960
BsrBI CCGCTC 1 cut(s) 656
BsrDI GCAATG 4 cut(s) 267, 482, 1024, 1312
BsrI ACTGG 3 cut(s) 105, 568, 976
BssECI CCNNGG 5 cut(s) 29, 54, 130, 580, 1187
BssMI GATC 4 cut(s) 174, 199, 658, 952
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 1187
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 582, 698
Bst4CI ACNGT 2 cut(s) 421, 1087
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 4 cut(s) 137, 280, 350, 645
BstDEI CTNAG 2 cut(s) 14, 936
BstDSI CCRYGG 2 cut(s) 54, 130
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 734
BstKTI GATC 4 cut(s) 177, 202, 661, 955
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 4 cut(s) 174, 199, 658, 952
BstMWI GCNNNNNNNGC 8 cut(s) 244, 611, 653, 731, 938, 966, 1227, 1236
BstNI CCWGG 2 cut(s) 582, 698
BstNSI RCATGY 4 cut(s) 155, 282, 527, 729
BstSCI CCNGG 2 cut(s) 580, 696
BstSFI CTRYAG 1 cut(s) 1083
BstSLI GKGCMC 1 cut(s) 1024
BstUI CGCG 1 cut(s) 734
BstV2I GAAGAC 1 cut(s) 1317
BstX2I RGATCY 1 cut(s) 952
BstXI CCANNNNNNTGG 2 cut(s) 112, 196
BstYI RGATCY 1 cut(s) 952
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 7 cut(s) 53, 129, 372, 512, 772, 1148, 1239
BtgI CCRYGG 2 cut(s) 54, 130
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 3 cut(s) 112, 417, 1219
Cac8I GCNNGC 4 cut(s) 137, 280, 350, 645
CaiI CAGNNNCTG 2 cut(s) 13, 1091
Cfr13I GGNCC 6 cut(s) 51, 115, 510, 998, 1110, 1237
CseI GACGC 1 cut(s) 619
CsiI ACCWGGT 1 cut(s) 696
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 8 cut(s) 152, 190, 266, 279, 524, 726, 850, 1002
DdeI CTNAG 2 cut(s) 14, 936
DpnI GATC 4 cut(s) 176, 201, 660, 954
DpnII GATC 4 cut(s) 174, 199, 658, 952
DrdI GACNNNNNNGTC 1 cut(s) 1005
DseDI GACNNNNNNGTC 1 cut(s) 1005
EaeI YGGCCR 2 cut(s) 370, 770
EciI GGCGGA 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 1187
Eco24I GRGCYC 2 cut(s) 547, 1256
Eco47I GGWCC 3 cut(s) 115, 998, 1110
Eco57I CTGAAG 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 1237
EcoRII CCWGG 2 cut(s) 580, 696
EcoT14I CCWWGG 1 cut(s) 1187
EcoT22I ATGCAT 2 cut(s) 153, 267
EcoT38I GRGCYC 2 cut(s) 547, 1256
ErhI CCWWGG 1 cut(s) 1187
FaeI CATG 8 cut(s) 155, 193, 269, 282, 527, 729, 853, 1005
FaqI GGGAC 3 cut(s) 80, 1073, 1161
FatI CATG 8 cut(s) 151, 189, 265, 278, 523, 725, 849, 1001
FauI CCCGC 1 cut(s) 533
FauNDI CATATG 1 cut(s) 965
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 2 cut(s) 547, 1256
FspBI CTAG 1 cut(s) 1127
HaeIII GGCC 7 cut(s) 53, 129, 372, 512, 772, 1148, 1239
HapII CCGG 2 cut(s) 215, 303
HgaI GACGC 1 cut(s) 619
Hin1II CATG 8 cut(s) 155, 193, 269, 282, 527, 729, 853, 1005
HindIII AAGCTT 2 cut(s) 137, 816
HinfI GANTC 7 cut(s) 226, 241, 326, 674, 687, 842, 1174
HpaII CCGG 2 cut(s) 215, 303
HphI GGTGA 3 cut(s) 768, 932, 1111
Hpy166II GTNNAC 1 cut(s) 1022
Hpy188I TCNGA 7 cut(s) 174, 379, 635, 708, 910, 937, 1110
Hpy188III TCNNGA 7 cut(s) 32, 83, 215, 806, 871, 1197, 1284
Hpy8I GTNNAC 1 cut(s) 1022
HpyAV CCTTC 5 cut(s) 756, 823, 868, 1211, 1281
HpyCH4III ACNGT 2 cut(s) 421, 1087
HpyF10VI GCNNNNNNNGC 8 cut(s) 244, 611, 653, 731, 938, 966, 1227, 1236
HpyF3I CTNAG 2 cut(s) 14, 936
Hsp92II CATG 8 cut(s) 155, 193, 269, 282, 527, 729, 853, 1005
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 174, 199, 658, 952
LmnI GCTCC 3 cut(s) 305, 473, 653
LweI GCATC 1 cut(s) 1125
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 1 cut(s) 1127
MalI GATC 4 cut(s) 176, 201, 660, 954
MbiI CCGCTC 1 cut(s) 656
MboI GATC 4 cut(s) 174, 199, 658, 952
MboII GAAGA 3 cut(s) 351, 1275, 1322
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 3 cut(s) 547, 1024, 1256
MlsI TGGCCA 2 cut(s) 372, 772
MluCI AATT 4 cut(s) 427, 668, 987, 1279
MluNI TGGCCA 2 cut(s) 372, 772
MlyI GAGTC 3 cut(s) 235, 668, 1183
MmeI TCCRAC 3 cut(s) 804, 976, 1088
Mox20I TGGCCA 2 cut(s) 372, 772
Mph1103I ATGCAT 2 cut(s) 153, 267
MroI TCCGGA 1 cut(s) 214
MscI TGGCCA 2 cut(s) 372, 772
MseI TTAA 3 cut(s) 332, 801, 836
MslI CAYNNNNRTG 1 cut(s) 909
Msp20I TGGCCA 2 cut(s) 372, 772
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 2 cut(s) 215, 303
MspR9I CCNGG 2 cut(s) 582, 698
MunI CAATTG 1 cut(s) 987
Mva1269I GAATGC 2 cut(s) 904, 1307
MvaI CCWGG 2 cut(s) 582, 698
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 8 cut(s) 244, 611, 653, 731, 938, 966, 1227, 1236
NdeI CATATG 1 cut(s) 965
NdeII GATC 4 cut(s) 174, 199, 658, 952
NlaIII CATG 8 cut(s) 155, 193, 269, 282, 527, 729, 853, 1005
NlaIV GGNNCC 1 cut(s) 52
NmeAIII GCCGAG 1 cut(s) 538
NsiI ATGCAT 2 cut(s) 153, 267
NspI RCATGY 4 cut(s) 155, 282, 527, 729
PaeI GCATGC 1 cut(s) 282
PctI GAATGC 2 cut(s) 904, 1307
PfeI GAWTC 4 cut(s) 226, 326, 687, 842
PleI GAGTC 3 cut(s) 235, 668, 1182
PpsI GAGTC 3 cut(s) 235, 668, 1182
Psp6I CCWGG 2 cut(s) 580, 696
PspGI CCWGG 2 cut(s) 580, 696
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 6 cut(s) 51, 115, 510, 998, 1110, 1237
PstNI CAGNNNCTG 2 cut(s) 13, 1091
PsuI RGATCY 1 cut(s) 952
RseI CAYNNNNRTG 1 cut(s) 909
SaqAI TTAA 3 cut(s) 332, 801, 836
Sau3AI GATC 4 cut(s) 174, 199, 658, 952
Sau96I GGNCC 6 cut(s) 51, 115, 510, 998, 1110, 1237
SchI GAGTC 3 cut(s) 235, 668, 1183
ScrFI CCNGG 2 cut(s) 582, 698
SduI GDGCHC 3 cut(s) 547, 1024, 1256
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 1 cut(s) 1125
SfcI CTRYAG 1 cut(s) 1083
SinI GGWCC 3 cut(s) 115, 998, 1110
SmiMI CAYNNNNRTG 1 cut(s) 909
SpeI ACTAGT 1 cut(s) 1126
SphI GCATGC 1 cut(s) 282
Sse9I AATT 4 cut(s) 427, 668, 987, 1279
SsiI CCGC 7 cut(s) 21, 245, 402, 405, 540, 656, 732
SspI AATATT 1 cut(s) 457
SspMI CTAG 1 cut(s) 1127
StyD4I CCNGG 2 cut(s) 580, 696
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 2 cut(s) 421, 1087
TaqI TCGA 2 cut(s) 177, 324
TaqII GACCGA 1 cut(s) 192
TasI AATT 4 cut(s) 427, 668, 987, 1279
TauI GCSGC 2 cut(s) 407, 734
TfiI GAWTC 4 cut(s) 226, 326, 687, 842
Tru1I TTAA 3 cut(s) 332, 801, 836
Tru9I TTAA 3 cut(s) 332, 801, 836
TscAI CASTG 3 cut(s) 112, 424, 1219
TspDTI ATGAA 3 cut(s) 587, 738, 1323
TspRI CASTG 3 cut(s) 112, 424, 1219
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 3 cut(s) 115, 998, 1110
XapI RAATTY 1 cut(s) 427
XceI RCATGY 4 cut(s) 155, 282, 527, 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 1156
XspI CTAG 1 cut(s) 1127
Zsp2I ATGCAT 2 cut(s) 153, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.