RLG00000024550

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001131
Physical Location & Seq
Forward (+)
36934016 .. 36937485
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024550

Sequence Viewer

Length: 987 bp
ATGGGTCAGGGGCTGAGTTGCGGAGAGCCTCGGGCGATTGGTCTGTTTGGGGCTGTGGAAAATGGGGACTTGGAGGTGGTTCAGGATATGGTGGAAGCTGACCCGAGTGTTTTGGGCCAGAGAAGAGGCCGTGGCAAGCTTTCTGCTATGCATGTAGCTGCTGCTGGTGGTCAGATCGAGGTGCTTTCCATGCTTTTGGATCGGTCTGTTAGTCCGGATGTAGTGAATCGCCATAAGCAGACTCCGCTGATGTTGGCTGCAATGCATGGGAATGTCACTTGTGTTAGAAAGCTAATCCAAGCAGGAGCAAATATATTGATGTTCGATTCCTTAAATGGAAGAACTTGCCTGCATTATGCTGCATATTATGGCCATTCAGACTGCCTCCAAGCCTTTTTATCCGCCGCACAATCCACCACTGTTGCAAATTCTTGGGGATTTGCAAGATTTGTGAATATTAGAGATGGAGGTGGAGCAACCCCATTGCACTTGGCTGCCCGTCAAAAACGGGCCGAGTGTGTGCATGTTCTTTTGGATAGTGGGGCTCTTGTTTGTGCTTCAACTGGTGGATACAGCTACCCTGGAAGCACACCACTTCATTTAGCTGCTCGTGCTGGTTCTTTAGATTGTGTCAGAGAGTTGCTTGCTTGGGGAGCAGATCGGCTTCAATTAGACTCATCAGGGAGAATCCCATACCTGGTTGCTCTGAAGCACAAGCACAGAGCATGTGCCGCGATACTGAACCCTTCATCAGCAGAGCCTCTTGTCTGGCCATCACCTTTGAAGTTTATCAGCGAACTTAATCCAGAGGCTAAAGCTTTGTTGGAGAAGGCTTTAATGGAATCAAACATGGAGAGGGAGAAAGCTATCTTGAAGGAGATAGTCTCCATTCCATCTCCTTTGCATTCAGATGTAGAGGGTGATGATAATGCCTCTGAGGTAAATGTGCCTTCTCTTTCTGCTCACTGTATTATTCTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

329

Amino Acids

34.89

Weight (kDa)

6.45

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 106 6e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 50 - 99 2.7e-08 Ankyrin repeats (many copies)
Ank PF00023 80 - 107 2.5e-06 Ankyrin repeat
Ank_4 PF13637 85 - 133 7.8e-11 Ankyrin repeats (many copies)
Ank_2 PF12796 112 - 183 2.4e-11 Ankyrin repeats (3 copies)
Ank PF00023 158 - 187 8.1e-06 Ankyrin repeat
Ank_2 PF12796 195 - 248 8.2e-07 Ankyrin repeats (3 copies)
Ank PF00023 195 - 225 1.7e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 734
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 5 cut(s) 21, 245, 402, 405, 732
AclWI GGATC 1 cut(s) 207
AcoI YGGCCR 2 cut(s) 370, 770
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 728
AfiI CCNNNNNNNGG 1 cut(s) 697
AgsI TTSAA 4 cut(s) 561, 668, 784, 874
AjnI CCWGG 2 cut(s) 580, 696
AluBI AGCT 8 cut(s) 98, 139, 158, 292, 576, 605, 818, 866
AluI AGCT 8 cut(s) 98, 139, 158, 292, 576, 605, 818, 866
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 1 cut(s) 207
AlwNI CAGNNNCTG 1 cut(s) 13
Ama87I CYCGRG 2 cut(s) 30, 103
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 5 cut(s) 115, 127, 370, 510, 770
ApeKI GCWGC 7 cut(s) 158, 161, 257, 359, 494, 605, 980
ApoI RAATTY 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 115, 510
AsuHPI GGTGA 2 cut(s) 768, 932
AvaI CYCGRG 2 cut(s) 30, 103
BalI TGGCCA 2 cut(s) 372, 772
BanII GRGCYC 1 cut(s) 547
BauI CACGAG 1 cut(s) 609
BbvI GCAGC 7 cut(s) 145, 148, 244, 346, 481, 592, 967
BccI CCATC 3 cut(s) 458, 781, 901
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 2 cut(s) 582, 698
BciVI GTATCC 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 889
BfuI GTATCC 1 cut(s) 563
BisI GCNGC 9 cut(s) 159, 162, 258, 360, 405, 495, 606, 732, 981
BlsI GCNGC 9 cut(s) 160, 163, 259, 361, 406, 496, 607, 733, 982
Bme1390I CCNGG 2 cut(s) 582, 698
BmeT110I CYCGRG 2 cut(s) 30, 103
BmgT120I GGNCC 2 cut(s) 115, 510
BmrFI CCNGG 2 cut(s) 582, 698
BplI GAGNNNNNCTC 2 cut(s) 869, 901
BsaJI CCNNGG 3 cut(s) 29, 130, 580
BsaWI WCCGGW 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 2 cut(s) 267, 482
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 2 cut(s) 582, 698
BseDI CCNNGG 3 cut(s) 29, 130, 580
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 697
BseMI GCAATG 2 cut(s) 267, 482
BseMII CTCAG 2 cut(s) 5, 927
BseNI ACTGG 1 cut(s) 568
BseXI GCAGC 7 cut(s) 145, 148, 244, 346, 481, 592, 967
Bsh1236I CGCG 1 cut(s) 734
BshFI GGCC 5 cut(s) 117, 129, 372, 512, 772
BsiHKCI CYCGRG 2 cut(s) 30, 103
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 697
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 904
BsnI GGCC 5 cut(s) 117, 129, 372, 512, 772
BsoBI CYCGRG 2 cut(s) 30, 103
Bsp1286I GDGCHC 1 cut(s) 547
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 3 cut(s) 174, 199, 658
BspACI CCGC 5 cut(s) 21, 245, 402, 405, 732
BspANI GGCC 5 cut(s) 117, 129, 372, 512, 772
BspCNI CTCAG 2 cut(s) 6, 928
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 1 cut(s) 734
BspPI GGATC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 267, 482
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 29, 130, 580
BssMI GATC 3 cut(s) 174, 199, 658
BssSI CACGAG 1 cut(s) 609
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 582, 698
Bst4CI ACNGT 2 cut(s) 421, 968
Bst6I CTCTTC 1 cut(s) 118
BstC8I GCNNGC 3 cut(s) 137, 350, 645
BstDEI CTNAG 2 cut(s) 14, 936
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 734
BstKTI GATC 3 cut(s) 177, 202, 661
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 3 cut(s) 174, 199, 658
BstMWI GCNNNNNNNGC 5 cut(s) 190, 244, 611, 653, 731
BstNI CCWGG 2 cut(s) 582, 698
BstNSI RCATGY 3 cut(s) 155, 527, 729
BstSCI CCNGG 2 cut(s) 580, 696
BstUI CGCG 1 cut(s) 734
BstV1I GCAGC 7 cut(s) 145, 148, 244, 346, 481, 592, 967
BstXI CCANNNNNNTGG 1 cut(s) 196
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 5 cut(s) 117, 129, 372, 512, 772
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 417, 964
Cac8I GCNNGC 3 cut(s) 137, 350, 645
CaiI CAGNNNCTG 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 115, 510
CsiI ACCWGGT 1 cut(s) 696
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 6 cut(s) 152, 190, 266, 524, 726, 850
DdeI CTNAG 2 cut(s) 14, 936
DpnI GATC 3 cut(s) 176, 201, 660
DpnII GATC 3 cut(s) 174, 199, 658
EaeI YGGCCR 2 cut(s) 370, 770
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 728
Eco88I CYCGRG 2 cut(s) 30, 103
EcoRII CCWGG 2 cut(s) 580, 696
EcoT22I ATGCAT 2 cut(s) 153, 267
EcoT38I GRGCYC 1 cut(s) 547
FaeI CATG 6 cut(s) 155, 193, 269, 527, 729, 853
FaqI GGGAC 1 cut(s) 80
FatI CATG 6 cut(s) 151, 189, 265, 523, 725, 849
Fnu4HI GCNGC 9 cut(s) 159, 162, 258, 360, 405, 495, 606, 732, 981
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 9 cut(s) 159, 162, 258, 360, 405, 495, 606, 732, 981
GluI GCNGC 9 cut(s) 159, 162, 258, 360, 405, 495, 606, 732, 981
HaeIII GGCC 5 cut(s) 117, 129, 372, 512, 772
HapII CCGG 1 cut(s) 215
Hin1II CATG 6 cut(s) 155, 193, 269, 527, 729, 853
HindIII AAGCTT 2 cut(s) 137, 816
HinfI GANTC 6 cut(s) 226, 241, 326, 674, 687, 842
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 768, 932
Hpy188I TCNGA 6 cut(s) 174, 379, 635, 708, 910, 937
Hpy188III TCNNGA 4 cut(s) 83, 215, 806, 871
HpyAV CCTTC 4 cut(s) 756, 823, 868, 960
HpyCH4III ACNGT 2 cut(s) 421, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 244, 611, 653, 731
HpyF3I CTNAG 2 cut(s) 14, 936
Hsp92II CATG 6 cut(s) 155, 193, 269, 527, 729, 853
Kpn2I TCCGGA 1 cut(s) 214
Kzo9I GATC 3 cut(s) 174, 199, 658
LmnI GCTCC 3 cut(s) 305, 473, 653
Lsp1109I GCAGC 7 cut(s) 145, 148, 244, 346, 481, 592, 967
MabI ACCWGGT 1 cut(s) 696
MaeIII GTNAC 1 cut(s) 274
MalI GATC 3 cut(s) 176, 201, 660
MboI GATC 3 cut(s) 174, 199, 658
MboII GAAGA 2 cut(s) 135, 351
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 2 cut(s) 372, 772
MluCI AATT 2 cut(s) 427, 668
MluNI TGGCCA 2 cut(s) 372, 772
MlyI GAGTC 2 cut(s) 235, 668
MmeI TCCRAC 1 cut(s) 804
Mox20I TGGCCA 2 cut(s) 372, 772
Mph1103I ATGCAT 2 cut(s) 153, 267
MroI TCCGGA 1 cut(s) 214
MscI TGGCCA 2 cut(s) 372, 772
MseI TTAA 3 cut(s) 332, 801, 836
MslI CAYNNNNRTG 2 cut(s) 270, 909
Msp20I TGGCCA 2 cut(s) 372, 772
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 582, 698
Mva1269I GAATGC 1 cut(s) 904
MvaI CCWGG 2 cut(s) 582, 698
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 5 cut(s) 190, 244, 611, 653, 731
NdeII GATC 3 cut(s) 174, 199, 658
NlaIII CATG 6 cut(s) 155, 193, 269, 527, 729, 853
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 274
NsiI ATGCAT 2 cut(s) 153, 267
NspI RCATGY 3 cut(s) 155, 527, 729
PctI GAATGC 1 cut(s) 904
PfeI GAWTC 4 cut(s) 226, 326, 687, 842
PkrI GCNGC 9 cut(s) 160, 163, 259, 361, 406, 496, 607, 733, 982
PleI GAGTC 2 cut(s) 235, 668
PpsI GAGTC 2 cut(s) 235, 668
Psp6I CCWGG 2 cut(s) 580, 696
PspGI CCWGG 2 cut(s) 580, 696
PspPI GGNCC 2 cut(s) 115, 510
PstNI CAGNNNCTG 1 cut(s) 13
RseI CAYNNNNRTG 2 cut(s) 270, 909
SaqAI TTAA 3 cut(s) 332, 801, 836
SatI GCNGC 9 cut(s) 159, 162, 258, 360, 405, 495, 606, 732, 981
Sau3AI GATC 3 cut(s) 174, 199, 658
Sau96I GGNCC 2 cut(s) 115, 510
SchI GAGTC 2 cut(s) 235, 668
ScrFI CCNGG 2 cut(s) 582, 698
SduI GDGCHC 1 cut(s) 547
SexAI ACCWGGT 1 cut(s) 696
SmiMI CAYNNNNRTG 2 cut(s) 270, 909
Sse9I AATT 2 cut(s) 427, 668
SsiI CCGC 5 cut(s) 21, 245, 402, 405, 732
SspI AATATT 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 580, 696
TaaI ACNGT 2 cut(s) 421, 968
TaqI TCGA 2 cut(s) 177, 324
TaqII GACCGA 1 cut(s) 192
TasI AATT 2 cut(s) 427, 668
TauI GCSGC 2 cut(s) 407, 734
TfiI GAWTC 4 cut(s) 226, 326, 687, 842
Tru1I TTAA 3 cut(s) 332, 801, 836
Tru9I TTAA 3 cut(s) 332, 801, 836
TscAI CASTG 2 cut(s) 424, 971
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 7 cut(s) 158, 161, 257, 359, 494, 605, 980
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 587, 738
TspRI CASTG 2 cut(s) 424, 971
XapI RAATTY 1 cut(s) 427
XceI RCATGY 3 cut(s) 155, 527, 729
Zsp2I ATGCAT 2 cut(s) 153, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.