Belongs to the thiolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 127 |
| AccB1I | GGYRCC | 1 cut(s) | 127 |
| AccIII | TCCGGA | 1 cut(s) | 107 |
| AfaI | GTAC | 1 cut(s) | 129 |
| AgsI | TTSAA | 1 cut(s) | 74 |
| AluBI | AGCT | 2 cut(s) | 38, 173 |
| AluI | AGCT | 2 cut(s) | 38, 173 |
| Aor13HI | TCCGGA | 1 cut(s) | 107 |
| ApeKI | GCWGC | 2 cut(s) | 35, 148 |
| Asp718I | GGTACC | 1 cut(s) | 127 |
| BanI | GGYRCC | 1 cut(s) | 127 |
| BauI | CACGAG | 1 cut(s) | 165 |
| BbvI | GCAGC | 2 cut(s) | 47, 135 |
| BccI | CCATC | 1 cut(s) | 23 |
| BfaI | CTAG | 1 cut(s) | 50 |
| BisI | GCNGC | 2 cut(s) | 36, 149 |
| BlsI | GCNGC | 2 cut(s) | 37, 150 |
| BmiI | GGNNCC | 1 cut(s) | 129 |
| BpuEI | CTTGAG | 1 cut(s) | 40 |
| BsaWI | WCCGGW | 1 cut(s) | 107 |
| BseAI | TCCGGA | 1 cut(s) | 107 |
| BseXI | GCAGC | 2 cut(s) | 47, 135 |
| BsgI | GTGCAG | 1 cut(s) | 54 |
| BshNI | GGYRCC | 1 cut(s) | 127 |
| BsiSI | CCGG | 1 cut(s) | 108 |
| Bsp13I | TCCGGA | 1 cut(s) | 107 |
| BspEI | TCCGGA | 1 cut(s) | 107 |
| BspLI | GGNNCC | 1 cut(s) | 129 |
| BspQI | GCTCTTC | 1 cut(s) | 147 |
| BspT107I | GGYRCC | 1 cut(s) | 127 |
| BssSI | CACGAG | 1 cut(s) | 165 |
| Bst2BI | CACGAG | 1 cut(s) | 165 |
| Bst4CI | ACNGT | 1 cut(s) | 132 |
| Bst6I | CTCTTC | 1 cut(s) | 147 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 157 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 157 |
| BstNSI | RCATGY | 1 cut(s) | 89 |
| BstV1I | GCAGC | 2 cut(s) | 47, 135 |
| BtsI | GCAGTG | 1 cut(s) | 31 |
| BtsIMutI | CAGTG | 1 cut(s) | 31 |
| Csp6I | GTAC | 1 cut(s) | 128 |
| CviAII | CATG | 2 cut(s) | 86, 137 |
| CviJI | RGCY | 2 cut(s) | 38, 173 |
| CviKI_1 | RGCY | 2 cut(s) | 38, 173 |
| CviQI | GTAC | 1 cut(s) | 128 |
| Eam1104I | CTCTTC | 1 cut(s) | 147 |
| EarI | CTCTTC | 1 cut(s) | 147 |
| FaeI | CATG | 2 cut(s) | 89, 140 |
| FaiI | YATR | 3 cut(s) | 87, 138, 156 |
| FatI | CATG | 2 cut(s) | 85, 136 |
| Fnu4HI | GCNGC | 2 cut(s) | 36, 149 |
| Fsp4HI | GCNGC | 2 cut(s) | 36, 149 |
| FspBI | CTAG | 1 cut(s) | 50 |
| GluI | GCNGC | 2 cut(s) | 36, 149 |
| HapII | CCGG | 1 cut(s) | 108 |
| Hin1II | CATG | 2 cut(s) | 89, 140 |
| HindIII | AAGCTT | 1 cut(s) | 171 |
| HpaII | CCGG | 1 cut(s) | 108 |
| Hpy188III | TCNNGA | 3 cut(s) | 19, 108, 167 |
| HpyCH4III | ACNGT | 1 cut(s) | 132 |
| HpyCH4V | TGCA | 2 cut(s) | 35, 151 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 157 |
| Hsp92II | CATG | 2 cut(s) | 89, 140 |
| Kpn2I | TCCGGA | 1 cut(s) | 107 |
| KpnI | GGTACC | 1 cut(s) | 131 |
| LguI | GCTCTTC | 1 cut(s) | 147 |
| LpnPI | CCDG | 1 cut(s) | 121 |
| Lsp1109I | GCAGC | 2 cut(s) | 47, 135 |
| MaeI | CTAG | 1 cut(s) | 50 |
| MaeIII | GTNAC | 1 cut(s) | 81 |
| MboII | GAAGA | 2 cut(s) | 89, 134 |
| MnlI | CCTC | 1 cut(s) | 115 |
| MroI | TCCGGA | 1 cut(s) | 107 |
| MspI | CCGG | 1 cut(s) | 108 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 157 |
| NlaIII | CATG | 2 cut(s) | 89, 140 |
| NlaIV | GGNNCC | 1 cut(s) | 129 |
| NspI | RCATGY | 1 cut(s) | 89 |
| PciSI | GCTCTTC | 1 cut(s) | 147 |
| PkrI | GCNGC | 2 cut(s) | 37, 150 |
| PspN4I | GGNNCC | 1 cut(s) | 129 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 66, 98 |
| RsaI | GTAC | 1 cut(s) | 129 |
| RsaNI | GTAC | 1 cut(s) | 128 |
| SapI | GCTCTTC | 1 cut(s) | 147 |
| SatI | GCNGC | 2 cut(s) | 36, 149 |
| SetI | ASST | 2 cut(s) | 40, 175 |
| SmlI | CTYRAG | 1 cut(s) | 19 |
| SmoI | CTYRAG | 1 cut(s) | 19 |
| SspMI | CTAG | 1 cut(s) | 50 |
| TaaI | ACNGT | 1 cut(s) | 132 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TseI | GCWGC | 2 cut(s) | 35, 148 |
| TspDTI | ATGAA | 2 cut(s) | 30, 89 |
| TspRI | CASTG | 1 cut(s) | 31 |
| XceI | RCATGY | 1 cut(s) | 89 |
| XspI | CTAG | 1 cut(s) | 50 |