MD13G1000800.v1.1

RNA splicing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002800
Physical Location & Seq
Reverse (-)
78547 .. 82171
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1000800.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCTTTTGCTTCTGCGACTTGCTTTTCTTCCCAACTGCAACTGCAACGGCTTCCCCGCGGCCTCGCCGCCAACAGTCCCGTATTCGTACTTCCTTCTCCTTCTTCTTCTAATTGTCGTCGTTTCAGACCTCTCAGTCTGACACTCAGAGCAAGCAGATCCTTTTCCGCCGTTGTATCGGCGGCGGCGGTGAAGAGTAATTCGCATTCGAATTCGAATTCCAGTGGAAACAAAACCAGTAAAGCAGCACCAGCGGAAGAAGAGGAAGCAGAAGAAGCAGTGGAAGAGGTGGAAGAGGATCTACCGTGGATTCAGGAGAAGGCCTTGGACGTGGTAGAGTTCACGGGCTCCGTCACACAGGCGATTCCTGGACCCAGAGTGGGCACCAGCTCTCTCCCTTGGATTCTGGCTCTCCCTCTCGCCTATGCCGGTCTCACTTTCGTCATTGCCCTTGTCAAGACCGTCAAAAAGTTTTCTTCCCCTCGACATAAACGCAAGAAGCTGGTGAATAAAAATGCAATGCTATGCAAATCAATAGACGAATTGTTTCAGAATGGTGCAGAAGTCAAACCCGATGTCTTGAACCAACTTGTGCAGAAGACTGGTTTTGGCATGGAGGAAATTTTGCGAAAGTACATTCGGTATACTTTGAATGAAAATCCATTCAACCCAGATGTGGTTTCCAACTTAATTCAGCTCAGAAAATCTTCCATGTTCGATGACTCTCAAGTCGCTGAAATTCTGAATGAAATTTCAAGAAGAATTGTACGAGACAAAGGCCCAGTTGTAATGGATGTTTCAGGCTACACTGAAAGAGGTTTCAAGAGAAAATTAGCTGTTCAAGCCCTGTTTGGAAAGGTTTTCTATCTGTCAGAGCTTCCAGAGTTCTGTTCGAGGGATAGCTCCTTAATTATCAAAGAAATATTTGGTGTTACAGATGAAGATGCAGATAAATTACGGATACATACGCTTTCTGAAGCAGTGGACATAGATTCGTTAGAAAAGATGGTTGATGGTTCAGATTCAGATGATTCTGGCCGGGGCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

38.52

Weight (kDa)

6.39

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARMH5 PF22915 201 - 292 2.8e-40 Armadillo-like helical domain-containing protein 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 135, 728
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 1 cut(s) 644
AccII CGCG 1 cut(s) 60
AciI CCGC 8 cut(s) 58, 60, 69, 168, 182, 185, 188, 254
AclWI GGATC 2 cut(s) 153, 306
AcoI YGGCCR 1 cut(s) 1036
AcsI RAATTY 5 cut(s) 211, 217, 621, 738, 750
AcuI CTGAAG 1 cut(s) 996
AfaI GTAC 3 cut(s) 90, 635, 768
AfiI CCNNNNNNNGG 2 cut(s) 380, 676
AgsI TTSAA 6 cut(s) 583, 652, 667, 756, 823, 842
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 6 cut(s) 390, 502, 697, 836, 877, 903
AluI AGCT 6 cut(s) 390, 502, 697, 836, 877, 903
Alw26I GTCTC 2 cut(s) 437, 765
AlwI GGATC 2 cut(s) 153, 306
AoxI GGCC 4 cut(s) 61, 321, 778, 1036
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 5 cut(s) 211, 217, 621, 738, 750
ArsI GACNNNNNNTTYG 2 cut(s) 561, 593
Asp700I GAANNNNTTC 2 cut(s) 546, 706
AspS9I GGNCC 2 cut(s) 371, 779
AsuC2I CCSGG 1 cut(s) 1040
AsuHPI GGTGA 2 cut(s) 202, 517
AsuII TTCGAA 2 cut(s) 209, 215
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 2 cut(s) 350, 1046
BarI GAAGNNNNNNTAC 4 cut(s) 75, 107, 285, 317
BbsI GAAGAC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 257
BccI CCATC 2 cut(s) 1000, 1007
BceAI ACGGC 2 cut(s) 65, 155
BciT130I CCWGG 1 cut(s) 369
BciVI GTATCC 1 cut(s) 954
BcnI CCSGG 1 cut(s) 1040
BcoDI GTCTC 2 cut(s) 437, 765
BfuI GTATCC 1 cut(s) 954
BisI GCNGC 5 cut(s) 61, 69, 183, 186, 246
BlsI GCNGC 5 cut(s) 62, 70, 184, 187, 247
Bme1390I CCNGG 2 cut(s) 369, 1040
Bme18I GGWCC 1 cut(s) 371
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 2 cut(s) 371, 779
BmiI GGNNCC 3 cut(s) 349, 373, 385
BmrFI CCNGG 2 cut(s) 369, 1040
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 1 cut(s) 934
BmuI ACTGGG 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 605
Bpu14I TTCGAA 2 cut(s) 209, 215
BpuEI CTTGAG 1 cut(s) 711
BpuMI CCSGG 1 cut(s) 1040
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 5 cut(s) 58, 305, 324, 398, 1039
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 676
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 4 cut(s) 222, 237, 607, 782
Bse3DI GCAATG 2 cut(s) 444, 525
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 5 cut(s) 58, 305, 324, 398, 1039
BseGI GGATG 1 cut(s) 799
BseLI CCNNNNNNNGG 2 cut(s) 380, 676
BseMI GCAATG 2 cut(s) 444, 525
BseMII CTCAG 3 cut(s) 148, 160, 712
BseNI ACTGG 4 cut(s) 222, 237, 607, 782
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 1 cut(s) 257
BsgI GTGCAG 2 cut(s) 579, 614
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 4 cut(s) 63, 323, 780, 1038
BshNI GGYRCC 1 cut(s) 383
BsiSI CCGG 2 cut(s) 429, 1039
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 380, 676
BsmAI GTCTC 2 cut(s) 437, 765
BsmFI GGGAC 1 cut(s) 63
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 4 cut(s) 63, 323, 780, 1038
Bso31I GGTCTC 1 cut(s) 437
Bsp119I TTCGAA 2 cut(s) 209, 215
Bsp1286I GDGCHC 3 cut(s) 350, 386, 1046
Bsp143I GATC 2 cut(s) 158, 298
BspACI CCGC 8 cut(s) 58, 60, 69, 168, 182, 185, 188, 254
BspANI GGCC 4 cut(s) 63, 323, 780, 1038
BspCNI CTCAG 3 cut(s) 147, 159, 711
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 349, 373, 385
BspPI GGATC 2 cut(s) 153, 306
BspT104I TTCGAA 2 cut(s) 209, 215
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 2 cut(s) 444, 525
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 4 cut(s) 222, 237, 607, 782
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 5 cut(s) 58, 305, 324, 398, 1039
BssMI GATC 2 cut(s) 158, 298
BssNAI GTATAC 1 cut(s) 645
BssT1I CCWWGG 2 cut(s) 324, 398
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 77, 306, 463
Bst6I CTCTTC 4 cut(s) 188, 255, 279, 288
BstBI TTCGAA 2 cut(s) 209, 215
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 3 cut(s) 134, 146, 698
BstDSI CCRYGG 2 cut(s) 58, 305
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 2 cut(s) 161, 301
BstMAI GTCTC 2 cut(s) 437, 765
BstMBI GATC 2 cut(s) 158, 298
BstMWI GCNNNNNNNGC 3 cut(s) 251, 275, 842
BstNI CCWGG 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 367, 1038
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 257
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 158, 298
BstYI RGATCY 2 cut(s) 158, 298
BstZ17I GTATAC 1 cut(s) 645
BsuI GTATCC 1 cut(s) 954
BsuRI GGCC 4 cut(s) 63, 323, 780, 1038
BtgI CCRYGG 2 cut(s) 58, 305
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 799
BtsI GCAGTG 2 cut(s) 285, 987
BtsIMutI CAGTG 4 cut(s) 229, 285, 807, 987
Cac8I GCNNGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 2 cut(s) 371, 779
Cfr42I CCGCGG 1 cut(s) 61
Csp6I GTAC 3 cut(s) 89, 634, 767
CviAII CATG 2 cut(s) 613, 712
CviQI GTAC 3 cut(s) 89, 634, 767
DdeI CTNAG 3 cut(s) 134, 146, 698
DpnI GATC 2 cut(s) 160, 300
DpnII GATC 2 cut(s) 158, 298
DrdI GACNNNNNNGTC 2 cut(s) 135, 728
DseDI GACNNNNNNGTC 2 cut(s) 135, 728
EaeI YGGCCR 1 cut(s) 1036
Eam1104I CTCTTC 4 cut(s) 188, 255, 279, 288
EarI CTCTTC 4 cut(s) 188, 255, 279, 288
EciI GGCGGA 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 324, 398
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 2 cut(s) 350, 1046
Eco31I GGTCTC 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 996
EcoRI GAATTC 2 cut(s) 211, 217
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 324, 398
EcoT38I GRGCYC 2 cut(s) 350, 1046
ErhI CCWWGG 2 cut(s) 324, 398
FaeI CATG 2 cut(s) 616, 715
FaiI YATR 8 cut(s) 426, 489, 526, 614, 645, 713, 966, 989
FaqI GGGAC 1 cut(s) 63
FatI CATG 2 cut(s) 612, 711
FauI CCCGC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 5 cut(s) 61, 69, 183, 186, 246
FokI GGATG 1 cut(s) 806
FriOI GRGCYC 2 cut(s) 350, 1046
Fsp4HI GCNGC 5 cut(s) 61, 69, 183, 186, 246
GluI GCNGC 5 cut(s) 61, 69, 183, 186, 246
HaeIII GGCC 4 cut(s) 63, 323, 780, 1038
HapII CCGG 2 cut(s) 429, 1039
Hin1II CATG 2 cut(s) 616, 715
HinfI GANTC 7 cut(s) 310, 364, 403, 722, 992, 1022, 1031
HpaII CCGG 2 cut(s) 429, 1039
HphI GGTGA 2 cut(s) 202, 517
Hpy166II GTNNAC 3 cut(s) 342, 645, 985
Hpy188III TCNNGA 6 cut(s) 314, 457, 580, 756, 823, 881
Hpy8I GTNNAC 3 cut(s) 342, 645, 985
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 3 cut(s) 105, 111, 313
HpyCH4III ACNGT 3 cut(s) 77, 306, 463
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 7 cut(s) 40, 46, 518, 528, 560, 595, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 275, 842
HpyF3I CTNAG 3 cut(s) 134, 146, 698
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 616, 715
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 2 cut(s) 158, 298
LmnI GCTCC 2 cut(s) 353, 908
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 934
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 352, 931
MalI GATC 2 cut(s) 160, 300
MboI GATC 2 cut(s) 158, 298
MflI RGATCY 2 cut(s) 158, 298
MhlI GDGCHC 3 cut(s) 350, 386, 1046
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 708
MroXI GAANNNNTTC 2 cut(s) 546, 706
MseI TTAA 2 cut(s) 689, 908
MspA1I CMGCKG 2 cut(s) 60, 254
MspI CCGG 2 cut(s) 429, 1039
MspR9I CCNGG 2 cut(s) 369, 1040
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 1 cut(s) 369
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 251, 275, 842
NciI CCSGG 1 cut(s) 1040
NdeII GATC 2 cut(s) 158, 298
NlaIII CATG 2 cut(s) 616, 715
NlaIV GGNNCC 3 cut(s) 349, 373, 385
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 2 cut(s) 209, 215
PceI AGGCCT 1 cut(s) 323
PctI GAATGC 1 cut(s) 205
PdmI GAANNNNTTC 2 cut(s) 546, 706
PfeI GAWTC 6 cut(s) 310, 364, 403, 992, 1022, 1031
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 5 cut(s) 62, 70, 184, 187, 247
PleI GAGTC 1 cut(s) 716
PpsI GAGTC 1 cut(s) 716
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 3 cut(s) 349, 373, 385
PspPI GGNCC 2 cut(s) 371, 779
PsuI RGATCY 2 cut(s) 158, 298
RsaI GTAC 3 cut(s) 90, 635, 768
RsaNI GTAC 3 cut(s) 89, 634, 767
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 2 cut(s) 689, 908
SatI GCNGC 5 cut(s) 61, 69, 183, 186, 246
Sau3AI GATC 2 cut(s) 158, 298
Sau96I GGNCC 2 cut(s) 371, 779
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 2 cut(s) 369, 1040
SduI GDGCHC 3 cut(s) 350, 386, 1046
SfaNI GCATC 1 cut(s) 934
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 2 cut(s) 209, 215
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
SseBI AGGCCT 1 cut(s) 323
SsiI CCGC 8 cut(s) 58, 60, 69, 168, 182, 185, 188, 254
SspI AATATT 1 cut(s) 924
StuI AGGCCT 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 367, 1038
StyI CCWWGG 2 cut(s) 324, 398
TaaI ACNGT 3 cut(s) 77, 306, 463
TaiI ACGT 1 cut(s) 333
TaqI TCGA 5 cut(s) 209, 215, 484, 717, 893
TatI WGTACW 1 cut(s) 633
TauI GCSGC 4 cut(s) 63, 71, 185, 188
TfiI GAWTC 6 cut(s) 310, 364, 403, 992, 1022, 1031
Tru1I TTAA 2 cut(s) 689, 908
Tru9I TTAA 2 cut(s) 689, 908
TscAI CASTG 4 cut(s) 229, 285, 814, 987
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 3 cut(s) 669, 762, 954
TspGWI ACGGA 2 cut(s) 340, 973
TspRI CASTG 4 cut(s) 229, 285, 814, 987
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 5 cut(s) 211, 217, 621, 738, 750
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 2 cut(s) 546, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.