Rh3AG256400

RNA splicing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002800
Physical Location & Seq
Forward (+)
28218466 .. 28284123
65658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG256400.1

Sequence Viewer

Length: 882 bp
ATGGCTTCCGCTTTGGCCACTTCCTTTGCTTCCCAATTACTCCCTCGCGCCCCACCCAAAGGTCTCCCTTTGCTTTGTCCCACAGTCACACCTGTGTCTGTAGCTGTGAGAGTCCGAGATAGCAGGCCGTTATCCGCTGTGGCGGCGGCGAAGAGTAATTCCAATCCTCCGCCGACGACGAGGAGCCCTAACAAAAGGAGTAAAGCAGCAGAAGAAGAAGAAGAAGTCGTTGAAGAAGTGGAAGAGGATCTACCCTGGATTCAAGAGAAGGCCTTGGACCTGGTGGATTTCACCGGCTCTGTCATGCAGGCCATTCCTGGACCCAGAGTTGGCACCAGCTCCCTCCCTTGGATTTTGGCTCTTCCTCTCACTTATGCCGGTCTCAATTTTTTCATCGGCGTTGTCAAGACCATCAAAAAGTTCTCTTCCCCTCGACATAAACGCAAGAACTGCCGTTCAAGCCCTATATGGAAAGGTGTTCTATCTGTCAGAGGTGCTACTAATAAGCCTCAAGAACTAGATGACGTAGTTCTTAGGAGACTGGTGAAGAGAGTATACATACCTTTACCAGATCTAACTGCTAGGAGACTCCTCCTAAGTCACAAACTCAAAGGCCAAGCATTTTCCTTACCAACCTGGGTCGGTTCTCCCTTCCCTTCCGCTTTGCTACTACTTATATGCAAGTTTGAGGTGGTGCCTATTACAGTTGGAAAGAAAGTGACTACAACCTTTTTGCGAGCCGCTACGCAAACTAATTTGACTGGGCTGCTTCACTATAATGGCCGCCTCAATCTCATACAATTCCTCATCCTTCGACTTAAACCAGACTCTTTCTCTTCCAAAAGCTTCTCCCAGCACCCAATTTCCTCCTCGCGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

32.16

Weight (kDa)

10.33

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 332, 694
AccI GTMKAC 1 cut(s) 555
AccII CGCG 2 cut(s) 48, 874
AciI CCGC 9 cut(s) 9, 135, 143, 146, 170, 660, 741, 784, 874
AclWI GGATC 1 cut(s) 255
AcoI YGGCCR 2 cut(s) 15, 781
AfiI CCNNNNNNNGG 4 cut(s) 59, 329, 348, 873
AgsI TTSAA 3 cut(s) 233, 263, 459
AjnI CCWGG 4 cut(s) 254, 279, 316, 635
AleI CACNNNNGTG 1 cut(s) 92
AluBI AGCT 3 cut(s) 104, 339, 846
AluI AGCT 3 cut(s) 104, 339, 846
Alw26I GTCTC 4 cut(s) 68, 386, 532, 580
AlwI GGATC 1 cut(s) 255
AoxI GGCC 6 cut(s) 15, 125, 270, 309, 613, 781
ApeKI GCWGC 2 cut(s) 206, 766
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 277, 320
AsuHPI GGTGA 2 cut(s) 283, 556
AvaII GGWCC 2 cut(s) 277, 320
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 2 cut(s) 332, 694
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 4 cut(s) 234, 266, 539, 571
BbvI GCAGC 2 cut(s) 218, 753
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 112, 438
BciT130I CCWGG 4 cut(s) 256, 281, 318, 637
BcoDI GTCTC 4 cut(s) 68, 386, 532, 580
BfaI CTAG 2 cut(s) 518, 582
BfmI CTRYAG 1 cut(s) 99
BglII AGATCT 1 cut(s) 571
BisI GCNGC 6 cut(s) 144, 147, 207, 741, 767, 784
BlsI GCNGC 6 cut(s) 145, 148, 208, 742, 768, 785
Bme1390I CCNGG 4 cut(s) 256, 281, 318, 637
Bme18I GGWCC 2 cut(s) 277, 320
BmgT120I GGNCC 2 cut(s) 277, 320
BmiI GGNNCC 4 cut(s) 185, 322, 334, 696
BmrFI CCNGG 4 cut(s) 256, 281, 318, 637
BmrI ACTGGG 1 cut(s) 771
BmuI ACTGGG 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 495
BsaI GGTCTC 2 cut(s) 68, 386
BsaJI CCNNGG 4 cut(s) 254, 273, 347, 636
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 329, 348, 873
Bse118I RCCGGY 2 cut(s) 293, 377
Bse1I ACTGG 2 cut(s) 546, 766
BseBI CCWGG 4 cut(s) 256, 281, 318, 637
BseDI CCNNGG 4 cut(s) 254, 273, 347, 636
BseGI GGATG 1 cut(s) 807
BseLI CCNNNNNNNGG 4 cut(s) 59, 329, 348, 873
BseNI ACTGG 2 cut(s) 546, 766
BseRI GAGGAG 3 cut(s) 196, 581, 859
BseXI GCAGC 2 cut(s) 218, 753
BseYI CCCAGC 1 cut(s) 852
Bsh1236I CGCG 2 cut(s) 48, 874
BshFI GGCC 6 cut(s) 17, 127, 272, 311, 615, 783
BshNI GGYRCC 2 cut(s) 332, 694
BsiSI CCGG 2 cut(s) 294, 378
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 4 cut(s) 59, 329, 348, 873
BsmAI GTCTC 4 cut(s) 68, 386, 532, 580
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 6 cut(s) 17, 127, 272, 311, 615, 783
Bso31I GGTCTC 2 cut(s) 68, 386
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 2 cut(s) 247, 571
BspACI CCGC 9 cut(s) 9, 135, 143, 146, 170, 660, 741, 784, 874
BspANI GGCC 6 cut(s) 17, 127, 272, 311, 615, 783
BspFNI CGCG 2 cut(s) 48, 874
BspLI GGNNCC 4 cut(s) 185, 322, 334, 696
BspPI GGATC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 366
BspT107I GGYRCC 2 cut(s) 332, 694
BspTNI GGTCTC 2 cut(s) 68, 386
BsrFI RCCGGY 2 cut(s) 293, 377
BsrI ACTGG 2 cut(s) 546, 766
BssAI RCCGGY 2 cut(s) 293, 377
BssECI CCNNGG 4 cut(s) 254, 273, 347, 636
BssMI GATC 2 cut(s) 247, 571
BssNAI GTATAC 1 cut(s) 556
BssT1I CCWWGG 2 cut(s) 273, 347
Bst1107I GTATAC 1 cut(s) 556
Bst2UI CCWGG 4 cut(s) 256, 281, 318, 637
Bst4CI ACNGT 2 cut(s) 85, 706
Bst6I CTCTTC 6 cut(s) 146, 237, 366, 430, 542, 841
BstAPI GCANNNNNTGC 1 cut(s) 450
BstC8I GCNNGC 3 cut(s) 125, 309, 738
BstDEI CTNAG 2 cut(s) 533, 596
BstF5I GGATG 1 cut(s) 807
BstFNI CGCG 2 cut(s) 48, 874
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 2 cut(s) 250, 574
BstMAI GTCTC 4 cut(s) 68, 386, 532, 580
BstMBI GATC 2 cut(s) 247, 571
BstMWI GCNNNNNNNGC 3 cut(s) 143, 450, 459
BstNI CCWGG 4 cut(s) 256, 281, 318, 637
BstSCI CCNGG 4 cut(s) 254, 279, 316, 635
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 2 cut(s) 48, 874
BstV1I GCAGC 2 cut(s) 218, 753
BstX2I RGATCY 2 cut(s) 247, 571
BstYI RGATCY 2 cut(s) 247, 571
BstZ17I GTATAC 1 cut(s) 556
BsuRI GGCC 6 cut(s) 17, 127, 272, 311, 615, 783
BtsCI GGATG 1 cut(s) 807
Cac8I GCNNGC 3 cut(s) 125, 309, 738
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 2 cut(s) 293, 377
Cfr13I GGNCC 2 cut(s) 277, 320
CsiI ACCWGGT 1 cut(s) 279
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 1 cut(s) 304
DdeI CTNAG 2 cut(s) 533, 596
DpnI GATC 2 cut(s) 249, 573
DpnII GATC 2 cut(s) 247, 571
EaeI YGGCCR 2 cut(s) 15, 781
Eam1104I CTCTTC 6 cut(s) 146, 237, 366, 430, 542, 841
EarI CTCTTC 6 cut(s) 146, 237, 366, 430, 542, 841
EciI GGCGGA 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 273, 347
Eco147I AGGCCT 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 68, 386
Eco47I GGWCC 2 cut(s) 277, 320
EcoRII CCWGG 4 cut(s) 254, 279, 316, 635
EcoT14I CCWWGG 2 cut(s) 273, 347
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 2 cut(s) 273, 347
FaeI CATG 1 cut(s) 307
FaqI GGGAC 1 cut(s) 63
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 867
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 6 cut(s) 144, 147, 207, 741, 767, 784
FokI GGATG 1 cut(s) 794
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 6 cut(s) 144, 147, 207, 741, 767, 784
FspBI CTAG 2 cut(s) 518, 582
GlaI GCGC 1 cut(s) 49
GluI GCNGC 6 cut(s) 144, 147, 207, 741, 767, 784
GsaI CCCAGC 1 cut(s) 856
HaeIII GGCC 6 cut(s) 17, 127, 272, 311, 615, 783
HapII CCGG 2 cut(s) 294, 378
HhaI GCGC 1 cut(s) 50
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 844
HinfI GANTC 4 cut(s) 111, 259, 588, 827
HpaII CCGG 2 cut(s) 294, 378
HphI GGTGA 2 cut(s) 283, 556
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 2 cut(s) 116, 491
Hpy188III TCNNGA 3 cut(s) 263, 406, 512
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 2 cut(s) 178, 181
HpyAV CCTTC 4 cut(s) 262, 661, 666, 821
HpyCH4III ACNGT 2 cut(s) 85, 706
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 2 cut(s) 307, 681
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 450, 459
HpyF3I CTNAG 2 cut(s) 533, 596
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 1 cut(s) 48
Kzo9I GATC 2 cut(s) 247, 571
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 183, 344
Lsp1109I GCAGC 2 cut(s) 218, 753
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 2 cut(s) 518, 582
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 3 cut(s) 85, 599, 718
MalI GATC 2 cut(s) 249, 573
MboI GATC 2 cut(s) 247, 571
MflI RGATCY 2 cut(s) 247, 571
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 6 cut(s) 35, 157, 385, 754, 800, 861
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 3 cut(s) 120, 582, 821
MmeI TCCRAC 1 cut(s) 688
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 2 cut(s) 92, 777
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 2 cut(s) 294, 378
MspR9I CCNGG 4 cut(s) 256, 281, 318, 637
MvaI CCWGG 4 cut(s) 256, 281, 318, 637
MvnI CGCG 2 cut(s) 48, 874
MwoI GCNNNNNNNGC 3 cut(s) 143, 450, 459
NdeII GATC 2 cut(s) 247, 571
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 4 cut(s) 185, 322, 334, 696
NmuCI GTSAC 3 cut(s) 85, 599, 718
OliI CACNNNNGTG 1 cut(s) 92
PceI AGGCCT 1 cut(s) 272
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 259
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 6 cut(s) 145, 148, 208, 742, 768, 785
PleI GAGTC 3 cut(s) 119, 582, 821
PpsI GAGTC 3 cut(s) 119, 582, 821
Psp6I CCWGG 4 cut(s) 254, 279, 316, 635
PspFI CCCAGC 1 cut(s) 852
PspGI CCWGG 4 cut(s) 254, 279, 316, 635
PspN4I GGNNCC 4 cut(s) 185, 322, 334, 696
PspPI GGNCC 2 cut(s) 277, 320
PsuI RGATCY 2 cut(s) 247, 571
RseI CAYNNNNRTG 2 cut(s) 92, 777
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 6 cut(s) 144, 147, 207, 741, 767, 784
Sau3AI GATC 2 cut(s) 247, 571
Sau96I GGNCC 2 cut(s) 277, 320
SchI GAGTC 3 cut(s) 120, 582, 821
ScrFI CCNGG 4 cut(s) 256, 281, 318, 637
SduI GDGCHC 1 cut(s) 188
SexAI ACCWGGT 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 277, 320
SmiMI CAYNNNNRTG 2 cut(s) 92, 777
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 6 cut(s) 35, 157, 385, 754, 800, 861
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 9 cut(s) 9, 135, 143, 146, 170, 660, 741, 784, 874
SspMI CTAG 2 cut(s) 518, 582
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 4 cut(s) 254, 279, 316, 635
StyI CCWWGG 2 cut(s) 273, 347
TaaI ACNGT 2 cut(s) 85, 706
TaiI ACGT 1 cut(s) 528
TaqI TCGA 2 cut(s) 433, 814
TasI AATT 6 cut(s) 35, 157, 385, 754, 800, 861
TauI GCSGC 4 cut(s) 146, 149, 743, 786
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TseFI GTSAC 3 cut(s) 85, 599, 718
TseI GCWGC 2 cut(s) 206, 766
Tsp45I GTSAC 3 cut(s) 85, 599, 718
TspDTI ATGAA 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 277, 320
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 2 cut(s) 518, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.