Rh4BG449200

RNA splicing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002800
Physical Location & Seq
Forward (+)
58715721 .. 58718240
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG449200.1

Sequence Viewer

Length: 993 bp
ATGGCTTCTGCTTTGGCTACTTCCTTTGCTTCCCAATTGCTCCCTCGCGCCCCACCCAAAGGTCTCCCTTTGCTTCGTCCCACAGTCGCACCTGTGTCTGTAGCTGTGAGAGTCCGAGCTAGCAGGCCGTTATCGGCGGCGGCGGCGGCGAAGAGTAATTCCAATCCTCCGCCCGCGACGACGAGCCCTAACAAAAGTAGTAAAGCAGCAGCAGAAGAAGAAGAAGGCGTTGAAGAAGTGGAAGAGGATCTACCCTGGATTCAAGAGAAGGCCTTGGACCTGGTGGAGTTCACCGGCTCTGTCACGCAGGCCATTCCTGGACCCAGAGTTGGCACCAGCTCGCTCCCTTGGATTTTGGCTCTTCCTCTCGCTTATGCCGGTCTCACTTTTGTCATCGCCGTTGTCAAGACCGTCAAAAAGTTCTCTTCCCCTCGACATAAACGCAAGAAACTGGTCAATAAAAATGCTATGCTATGCAAATCAATAGACGAAATGTTTCAGAATGGCTCCGACCAAATCACGCCTGATGCCCTGAAACAACTCGTGCAAAAGACAGGTTTTGGCATGGAGGAGATTTTGCGCAAGTACATCCGTTATACTTTGAATGAAAAGCCATTCAACCCAGACATGGTTTCCAATTTAATTCAGCTCAGGAAAGCTTCTTTATTCGATGATTCTCAGGTGGCTGAAATTCTAAATGAAATTTCAAGAAGAATTGTGCGAGACAAAGGCCCAGTTGTAATGGATATGGCAGGTTATACTGAACGGGGTTTTAAAAGAAAATTAGCCGTTCAAGCCCTATTTGGAAAGGTGTTCTATCTGTCAGAGCTCCCAGAGTTCTGTTCGAGGGATAGCTCCTTAATTGTCAAAGAAATATTTGGCGTTACAGATGAAGATGCAGACAGATTACGGATACATACAGCCTCTGAAGCTGGGGATATGGAATCACTGAAAAAAAAGTTTGATGTTTCAGATTCAGAAGATTCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

36.23

Weight (kDa)

8.67

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARMH5 PF22915 185 - 276 1.5e-40 Armadillo-like helical domain-containing protein 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 332
AccII CGCG 2 cut(s) 48, 176
AciI CCGC 6 cut(s) 137, 140, 143, 146, 170, 174
AclWI GGATC 1 cut(s) 255
AcsI RAATTY 2 cut(s) 690, 702
AcuI CTGAAG 1 cut(s) 948
AfaI GTAC 1 cut(s) 587
AfiI CCNNNNNNNGG 3 cut(s) 59, 329, 628
AgsI TTSAA 6 cut(s) 233, 263, 604, 619, 708, 794
AjnI CCWGG 3 cut(s) 254, 279, 316
AluBI AGCT 9 cut(s) 104, 119, 339, 649, 659, 829, 855, 932, 990
AluI AGCT 9 cut(s) 104, 119, 339, 649, 659, 829, 855, 932, 990
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 3 cut(s) 68, 386, 717
AlwI GGATC 1 cut(s) 255
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 4 cut(s) 125, 270, 309, 730
ApeKI GCWGC 2 cut(s) 206, 209
ApoI RAATTY 2 cut(s) 690, 702
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 50, 582
AspS9I GGNCC 3 cut(s) 277, 320, 731
AsuHPI GGTGA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 119
AvaII GGWCC 2 cut(s) 277, 320
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 2 cut(s) 188, 831
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
BauI CACGAG 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 2 cut(s) 218, 221
BceAI ACGGC 3 cut(s) 112, 383, 773
BciT130I CCWGG 3 cut(s) 256, 281, 318
BciVI GTATCC 1 cut(s) 906
BcoDI GTCTC 3 cut(s) 68, 386, 717
BfaI CTAG 3 cut(s) 120, 987, 991
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 743
BfuI GTATCC 1 cut(s) 906
BisI GCNGC 6 cut(s) 138, 141, 144, 147, 207, 210
BlsI GCNGC 6 cut(s) 139, 142, 145, 148, 208, 211
Bme1390I CCNGG 3 cut(s) 256, 281, 318
Bme18I GGWCC 2 cut(s) 277, 320
BmgT120I GGNCC 3 cut(s) 277, 320, 731
BmiI GGNNCC 3 cut(s) 322, 334, 508
BmrFI CCNGG 3 cut(s) 256, 281, 318
BmrI ACTGGG 1 cut(s) 728
BmsI GCATC 2 cut(s) 517, 886
BmtI GCTAGC 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 728
Bpu10I CCTNAGC 1 cut(s) 650
BsaI GGTCTC 2 cut(s) 68, 386
BsaJI CCNNGG 3 cut(s) 254, 273, 347
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 329, 628
Bse118I RCCGGY 2 cut(s) 293, 377
Bse1I ACTGG 2 cut(s) 456, 734
BseBI CCWGG 3 cut(s) 256, 281, 318
BseDI CCNNGG 3 cut(s) 254, 273, 347
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 59, 329, 628
BseMII CTCAG 2 cut(s) 664, 692
BseNI ACTGG 2 cut(s) 456, 734
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 2 cut(s) 218, 221
BseYI CCCAGC 1 cut(s) 932
Bsh1236I CGCG 2 cut(s) 48, 176
BshFI GGCC 4 cut(s) 127, 272, 311, 732
BshNI GGYRCC 1 cut(s) 332
BsiHKAI GWGCWC 1 cut(s) 831
BsiSI CCGG 2 cut(s) 294, 378
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 3 cut(s) 59, 329, 628
BsmAI GTCTC 3 cut(s) 68, 386, 717
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 4 cut(s) 127, 272, 311, 732
Bso31I GGTCTC 2 cut(s) 68, 386
Bsp1286I GDGCHC 2 cut(s) 188, 831
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 6 cut(s) 137, 140, 143, 146, 170, 174
BspANI GGCC 4 cut(s) 127, 272, 311, 732
BspCNI CTCAG 2 cut(s) 663, 691
BspFNI CGCG 2 cut(s) 48, 176
BspLI GGNNCC 3 cut(s) 322, 334, 508
BspMI ACCTGC 1 cut(s) 743
BspOI GCTAGC 1 cut(s) 123
BspPI GGATC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 332
BspTNI GGTCTC 2 cut(s) 68, 386
BsrFI RCCGGY 2 cut(s) 293, 377
BsrI ACTGG 2 cut(s) 456, 734
BssAI RCCGGY 2 cut(s) 293, 377
BssECI CCNNGG 3 cut(s) 254, 273, 347
BssMI GATC 1 cut(s) 247
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 2 cut(s) 273, 347
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 3 cut(s) 256, 281, 318
Bst4CI ACNGT 2 cut(s) 85, 412
Bst6I CTCTTC 4 cut(s) 146, 237, 366, 430
BstC8I GCNNGC 5 cut(s) 121, 125, 174, 309, 341
BstDEI CTNAG 2 cut(s) 650, 678
BstF5I GGATG 1 cut(s) 588
BstFNI CGCG 2 cut(s) 48, 176
BstHHI GCGC 2 cut(s) 50, 582
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 3 cut(s) 68, 386, 717
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 4 cut(s) 143, 146, 794, 929
BstNI CCWGG 3 cut(s) 256, 281, 318
BstSCI CCNGG 3 cut(s) 254, 279, 316
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 2 cut(s) 48, 176
BstV1I GCAGC 2 cut(s) 218, 221
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuI GTATCC 1 cut(s) 906
BsuRI GGCC 4 cut(s) 127, 272, 311, 732
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 947
BveI ACCTGC 1 cut(s) 743
Cac8I GCNNGC 5 cut(s) 121, 125, 174, 309, 341
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 2 cut(s) 50, 582
Cfr10I RCCGGY 2 cut(s) 293, 377
Cfr13I GGNCC 3 cut(s) 277, 320, 731
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 586
CviAII CATG 2 cut(s) 565, 628
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 2 cut(s) 650, 678
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 775
Eam1104I CTCTTC 4 cut(s) 146, 237, 366, 430
EarI CTCTTC 4 cut(s) 146, 237, 366, 430
EciI GGCGGA 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 829
Eco130I CCWWGG 2 cut(s) 273, 347
Eco147I AGGCCT 1 cut(s) 272
Eco24I GRGCYC 2 cut(s) 188, 831
Eco31I GGTCTC 2 cut(s) 68, 386
Eco47I GGWCC 2 cut(s) 277, 320
Eco53kI GAGCTC 1 cut(s) 829
Eco57I CTGAAG 1 cut(s) 948
EcoICRI GAGCTC 1 cut(s) 829
EcoRII CCWGG 3 cut(s) 254, 279, 316
EcoT14I CCWWGG 2 cut(s) 273, 347
EcoT38I GRGCYC 2 cut(s) 188, 831
ErhI CCWWGG 2 cut(s) 273, 347
FaeI CATG 2 cut(s) 568, 631
FaqI GGGAC 1 cut(s) 63
FatI CATG 2 cut(s) 564, 627
FauI CCCGC 1 cut(s) 181
Fnu4HI GCNGC 6 cut(s) 138, 141, 144, 147, 207, 210
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 2 cut(s) 188, 831
Fsp4HI GCNGC 6 cut(s) 138, 141, 144, 147, 207, 210
FspBI CTAG 3 cut(s) 120, 987, 991
FspI TGCGCA 1 cut(s) 581
GlaI GCGC 2 cut(s) 49, 581
GluI GCNGC 6 cut(s) 138, 141, 144, 147, 207, 210
GsaI CCCAGC 1 cut(s) 936
HaeIII GGCC 4 cut(s) 127, 272, 311, 732
HapII CCGG 2 cut(s) 294, 378
HhaI GCGC 2 cut(s) 50, 582
Hin1II CATG 2 cut(s) 568, 631
Hin6I GCGC 2 cut(s) 48, 580
HinP1I GCGC 2 cut(s) 48, 580
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 6 cut(s) 111, 259, 674, 944, 974, 983
HpaII CCGG 2 cut(s) 294, 378
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 7 cut(s) 116, 501, 511, 826, 928, 973, 979
Hpy188III TCNNGA 4 cut(s) 263, 406, 652, 708
Hpy8I GTNNAC 1 cut(s) 291
Hpy99I CGWCG 2 cut(s) 181, 184
HpyAV CCTTC 2 cut(s) 218, 262
HpyCH4III ACNGT 2 cut(s) 85, 412
HpyCH4V TGCA 3 cut(s) 477, 547, 899
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 146, 794, 929
HpyF3I CTNAG 2 cut(s) 650, 678
Hsp92II CATG 2 cut(s) 568, 631
HspAI GCGC 2 cut(s) 48, 580
Kzo9I GATC 1 cut(s) 247
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 5 cut(s) 45, 348, 512, 834, 860
Lsp1109I GCAGC 2 cut(s) 218, 221
LweI GCATC 2 cut(s) 517, 886
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 3 cut(s) 120, 987, 991
MaeIII GTNAC 2 cut(s) 301, 883
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 2 cut(s) 188, 831
MluCI AATT 9 cut(s) 35, 157, 637, 642, 690, 702, 714, 782, 861
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 8 cut(s) 54, 177, 238, 375, 441, 562, 840, 934
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 3 cut(s) 641, 774, 860
MspI CCGG 2 cut(s) 294, 378
MspR9I CCNGG 3 cut(s) 256, 281, 318
MunI CAATTG 1 cut(s) 35
MvaI CCWGG 3 cut(s) 256, 281, 318
MvnI CGCG 2 cut(s) 48, 176
MwoI GCNNNNNNNGC 4 cut(s) 143, 146, 794, 929
NdeII GATC 1 cut(s) 247
NheI GCTAGC 1 cut(s) 119
NlaIII CATG 2 cut(s) 568, 631
NlaIV GGNNCC 3 cut(s) 322, 334, 508
NmuCI GTSAC 1 cut(s) 301
NsbI TGCGCA 1 cut(s) 581
PceI AGGCCT 1 cut(s) 272
PciSI GCTCTTC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 5 cut(s) 259, 674, 944, 974, 983
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 6 cut(s) 139, 142, 145, 148, 208, 211
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 831
Psp6I CCWGG 3 cut(s) 254, 279, 316
PspFI CCCAGC 1 cut(s) 932
PspGI CCWGG 3 cut(s) 254, 279, 316
PspN4I GGNNCC 3 cut(s) 322, 334, 508
PspPI GGNCC 3 cut(s) 277, 320, 731
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
SacI GAGCTC 1 cut(s) 831
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 3 cut(s) 641, 774, 860
SatI GCNGC 6 cut(s) 138, 141, 144, 147, 207, 210
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 3 cut(s) 277, 320, 731
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 3 cut(s) 256, 281, 318
SduI GDGCHC 2 cut(s) 188, 831
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 2 cut(s) 517, 886
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 277, 320
Sse9I AATT 9 cut(s) 35, 157, 637, 642, 690, 702, 714, 782, 861
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 6 cut(s) 137, 140, 143, 146, 170, 174
SspI AATATT 1 cut(s) 876
SspMI CTAG 3 cut(s) 120, 987, 991
SstI GAGCTC 1 cut(s) 831
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 254, 279, 316
StyI CCWWGG 2 cut(s) 273, 347
TaaI ACNGT 2 cut(s) 85, 412
TaqI TCGA 3 cut(s) 433, 669, 845
TasI AATT 9 cut(s) 35, 157, 637, 642, 690, 702, 714, 782, 861
TatI WGTACW 1 cut(s) 585
TauI GCSGC 4 cut(s) 140, 143, 146, 149
TfiI GAWTC 5 cut(s) 259, 674, 944, 974, 983
Tru1I TTAA 3 cut(s) 641, 774, 860
Tru9I TTAA 3 cut(s) 641, 774, 860
TscAI CASTG 1 cut(s) 954
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 206, 209
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 3 cut(s) 621, 714, 906
TspGWI ACGGA 2 cut(s) 581, 925
TspRI CASTG 1 cut(s) 954
VpaK11BI GGWCC 2 cut(s) 277, 320
XapI RAATTY 2 cut(s) 690, 702
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 120, 987, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.