Rh4AG023500

RNA splicing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002800
Physical Location & Seq
Reverse (-)
4633042 .. 4633666
625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG023500.1

Sequence Viewer

Length: 546 bp
ATGGCTTTTGCTTTGGTCACTTCCTTTGCTTCCCAATTGCTCCCTCGCGCCCCACCCAAAGGTCTCCCTTTGCTTCGTCCCACAGTCGCACCTGTGTCTGTAGCTGTGAGAGTCCGAGCAAGCAAACTGTTATTCGCTGTGGCGGCGGTGAAGAATAATTCCAATCCTCCGCCGATGACGAAGAGCCCTAACAAAAGCAGTAAAGCAGAAGAAGAAGAAGAAGAAGAAGAAGCCGTTGAAGAAGTGGAAGAGGATCTACCTTGGATTCAAGAGAAGGCCTTGGACCTGGTGGAGTTCACCGACTCTGTCACGCAGGCCATTCCTGGACCCAGAGTTGGCACCAGCTCATTCCTTTGGATTTTGGCTTTTCCTCTCGCTTATGCCGGTCTCAATTTTGTCATCGCCGTTGTCAATACCGTCAAAAAGTTCTCTTCCCCTCGACATAAACGCAAGAAACTAGTCAATAAAAATGCTATGCTATGCAAATCAATAGACGAAATGTTTCAGAATGGCTCCGACCAAATCACGCCTGATGCCCTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.83

Weight (kDa)

7.8

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 1 cut(s) 48
AciI CCGC 3 cut(s) 143, 146, 170
AclWI GGATC 1 cut(s) 261
AfiI CCNNNNNNNGG 2 cut(s) 59, 335
AgsI TTSAA 2 cut(s) 239, 269
AhlI ACTAGT 1 cut(s) 457
AjnI CCWGG 2 cut(s) 285, 322
AluBI AGCT 2 cut(s) 104, 345
AluI AGCT 2 cut(s) 104, 345
Alw26I GTCTC 2 cut(s) 68, 392
AlwI GGATC 1 cut(s) 261
AoxI GGCC 2 cut(s) 276, 315
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 283, 326
AsuHPI GGTGA 2 cut(s) 160, 289
AvaII GGWCC 2 cut(s) 283, 326
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 240, 272
BceAI ACGGC 2 cut(s) 218, 389
BciT130I CCWGG 2 cut(s) 287, 324
BcoDI GTCTC 2 cut(s) 68, 392
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 287, 324
Bme18I GGWCC 2 cut(s) 283, 326
BmgT120I GGNCC 2 cut(s) 283, 326
BmiI GGNNCC 3 cut(s) 328, 340, 514
BmrFI CCNGG 2 cut(s) 287, 324
BmsI GCATC 1 cut(s) 523
BsaI GGTCTC 2 cut(s) 68, 392
BsaJI CCNNGG 2 cut(s) 260, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 335
Bse118I RCCGGY 1 cut(s) 383
BseBI CCWGG 2 cut(s) 287, 324
BseDI CCNNGG 2 cut(s) 260, 279
BseLI CCNNNNNNNGG 2 cut(s) 59, 335
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 278, 317
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 59, 335
BsmAI GTCTC 2 cut(s) 68, 392
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 2 cut(s) 278, 317
Bso31I GGTCTC 2 cut(s) 68, 392
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 3 cut(s) 143, 146, 170
BspANI GGCC 2 cut(s) 278, 317
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 328, 340, 514
BspPI GGATC 1 cut(s) 261
BspQI GCTCTTC 1 cut(s) 176
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 2 cut(s) 68, 392
BsrFI RCCGGY 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 2 cut(s) 260, 279
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 2 cut(s) 260, 279
Bst2UI CCWGG 2 cut(s) 287, 324
Bst4CI ACNGT 3 cut(s) 85, 129, 418
Bst6I CTCTTC 3 cut(s) 176, 243, 436
BstC8I GCNNGC 2 cut(s) 121, 315
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 2 cut(s) 68, 392
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 2 cut(s) 287, 324
BstSCI CCNGG 2 cut(s) 285, 322
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 2 cut(s) 278, 317
BtgZI GCGATG 1 cut(s) 385
Cac8I GCNNGC 2 cut(s) 121, 315
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 283, 326
CsiI ACCWGGT 1 cut(s) 285
CviJI RGCY 9 cut(s) 5, 104, 186, 233, 278, 317, 345, 365, 513
CviKI_1 RGCY 9 cut(s) 5, 104, 186, 233, 278, 317, 345, 365, 513
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 176, 243, 436
EarI CTCTTC 3 cut(s) 176, 243, 436
EciI GGCGGA 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 260, 279
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 2 cut(s) 68, 392
Eco47I GGWCC 2 cut(s) 283, 326
EcoRII CCWGG 2 cut(s) 285, 322
EcoT14I CCWWGG 2 cut(s) 260, 279
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 2 cut(s) 260, 279
FaiI YATR 4 cut(s) 381, 444, 476, 481
FaqI GGGAC 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 49
GluI GCNGC 1 cut(s) 144
HaeIII GGCC 2 cut(s) 278, 317
HapII CCGG 1 cut(s) 384
HhaI GCGC 1 cut(s) 50
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 3 cut(s) 111, 265, 302
HpaII CCGG 1 cut(s) 384
HphI GGTGA 2 cut(s) 160, 289
Hpy166II GTNNAC 1 cut(s) 297
Hpy188I TCNGA 3 cut(s) 116, 507, 517
Hpy188III TCNNGA 1 cut(s) 269
Hpy8I GTNNAC 1 cut(s) 297
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 3 cut(s) 85, 129, 418
HpyCH4V TGCA 1 cut(s) 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HspAI GCGC 1 cut(s) 48
Kzo9I GATC 1 cut(s) 253
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 45, 518
LpnPI CCDG 9 cut(s) 105, 272, 299, 299, 309, 336, 343, 355, 397
LweI GCATC 1 cut(s) 523
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 2 cut(s) 16, 307
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 3 cut(s) 35, 157, 391
MlyI GAGTC 2 cut(s) 120, 296
MmeI TCCRAC 1 cut(s) 540
MnlI CCTC 5 cut(s) 54, 177, 244, 381, 447
MroXI GAANNNNTTC 1 cut(s) 501
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 287, 324
MunI CAATTG 1 cut(s) 35
MvaI CCWGG 2 cut(s) 287, 324
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeII GATC 1 cut(s) 253
NlaIV GGNNCC 3 cut(s) 328, 340, 514
NmuCI GTSAC 2 cut(s) 16, 307
PceI AGGCCT 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 1 cut(s) 265
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 2 cut(s) 119, 296
PpsI GAGTC 2 cut(s) 119, 296
Psp6I CCWGG 2 cut(s) 285, 322
PspGI CCWGG 2 cut(s) 285, 322
PspN4I GGNNCC 3 cut(s) 328, 340, 514
PspPI GGNCC 2 cut(s) 283, 326
PsuI RGATCY 1 cut(s) 253
PsyI GACNNNGTC 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 176
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 283, 326
SchI GAGTC 2 cut(s) 120, 296
ScrFI CCNGG 2 cut(s) 287, 324
SduI GDGCHC 1 cut(s) 188
SetI ASST 6 cut(s) 64, 94, 106, 262, 288, 347
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 283, 326
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 3 cut(s) 35, 157, 391
SseBI AGGCCT 1 cut(s) 278
SsiI CCGC 3 cut(s) 143, 146, 170
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 2 cut(s) 285, 322
StyI CCWWGG 2 cut(s) 260, 279
TaaI ACNGT 3 cut(s) 85, 129, 418
TaqI TCGA 1 cut(s) 439
TasI AATT 3 cut(s) 35, 157, 391
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 265
TseFI GTSAC 2 cut(s) 16, 307
Tsp45I GTSAC 2 cut(s) 16, 307
Tth111I GACNNNGTC 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 283, 326
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.