MD16G1004900.v1.1

RNA splicing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002800
Physical Location & Seq
Forward (+)
373298 .. 376881
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1004900.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCTTTTGCTTCTGCGACTTGCTTTTCTTCCCAACTCGAACTGAAACTGCTTCCCCGCGGCCTCGCCGCCAACAGTCCCCTATTGGTATTTCCTTCTTATAATAGTCGTCGTTTCAGACCTCTCAGTCCGACACTCAAAGCAAGCAGACCCTTTTCCGGCGTTGTATCGGCTGCGGCGAAGGGTAATTCGCATTCGAATTCAAATTCCGTTGGAATCAAAACCAGTAAAGCAGCAGCGGAAGAAGAGGAAGTAGTGGAAGAGGTGGAAGAGGATTTACCGTGGATTCAGGAGAAGGCCTTGGACGTGGTAGAGTTCACGGGCTCCGTCACACAGGCGATTCCTGGACCCAGAGTGGGCACCAGCTCTCTCCCTTGGATTCTGGCTCTCCCTCTCACCTATGCCGGTCTCACTTTCGTCATTGCCCTTGTCAAGACCATCAAAAAGTTTTCTTCCCCTCGACATAAACGCAAGAAACTGGTGAATAAAAATGCTATGCTATGCAAATCAATAGACGAATTGTTTCAGAGGGGTGCAGATGAAGTCAAACCCGATGCCTTGCACCAGCTTGTGCAGAAGACTGGTTTTAGCATGGAGGAAATTTTGCGAAAGTACATTCGGTATACTTTGAATGAAAAACCATTCAACCCAGACGTGGTTTCCAACTTAATTCAGCTCAGAAAAGCTTCAATGTTCGATGATTCTCAAGTTGCTGAAATTCTGAATGAAATTTCAAGAAGAATTGTACGAGACAAGGGCCCAGTTGTAATGGATGTTTCAGGCTACACTGAAAGAGGTTTCAAGAGAAAATTAGCTGTTCAATCCCTTTTTGGAAAGGTTTTCTATCTGTCAGAGCTTCCAGAGTTCTGTTCGAGGGATAGCTCCTTAATTGTCAAAGAAATATTTGGCGTTACTGATGAAGATGCGGATAAGTTACGGATACATACACTTTCTGAAGCAGGGGATATAGATTCGTTAGAAAAGATGGTTGATGGTTCAGATTCAGATGATTCTGGTCACGGCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

37.88

Weight (kDa)

6.98

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARMH5 PF22915 194 - 285 1.5e-41 Armadillo-like helical domain-containing protein 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 623
AccII CGCG 1 cut(s) 60
AciI CCGC 6 cut(s) 58, 60, 69, 176, 239, 926
AcsI RAATTY 5 cut(s) 199, 205, 600, 717, 729
AcuI CTGAAG 1 cut(s) 975
AfaI GTAC 2 cut(s) 614, 747
AfiI CCNNNNNNNGG 3 cut(s) 158, 356, 655
AgsI TTSAA 7 cut(s) 204, 631, 646, 690, 735, 802, 821
AjiI CACGTC 2 cut(s) 307, 655
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 7 cut(s) 366, 568, 676, 686, 815, 856, 882
AluI AGCT 7 cut(s) 366, 568, 676, 686, 815, 856, 882
Alw26I GTCTC 2 cut(s) 413, 744
AoxI GGCC 3 cut(s) 61, 297, 757
ApaI GGGCCC 1 cut(s) 761
ApeKI GCWGC 3 cut(s) 173, 233, 236
ApoI RAATTY 5 cut(s) 199, 205, 600, 717, 729
Asp700I GAANNNNTTC 2 cut(s) 522, 685
AspS9I GGNCC 3 cut(s) 347, 757, 758
AsuHPI GGTGA 2 cut(s) 388, 493
AsuII TTCGAA 1 cut(s) 197
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 2 cut(s) 362, 761
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 326, 761
BarI GAAGNNNNNNTAC 2 cut(s) 261, 293
BbsI GAAGAC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 160, 245, 248
BccI CCATC 3 cut(s) 446, 979, 986
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 933
BcoDI GTCTC 2 cut(s) 413, 744
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 6 cut(s) 61, 69, 174, 177, 234, 237
BlsI GCNGC 6 cut(s) 62, 70, 175, 178, 235, 238
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 347
BmgBI CACGTC 2 cut(s) 307, 655
BmgT120I GGNCC 3 cut(s) 347, 757, 758
BmiI GGNNCC 4 cut(s) 325, 349, 361, 759
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 544, 913
BmuI ACTGGG 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 584
Bpu14I TTCGAA 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 4 cut(s) 58, 281, 300, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 356, 655
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 4 cut(s) 225, 483, 586, 761
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 4 cut(s) 58, 281, 300, 374
BseGI GGATG 1 cut(s) 778
BseLI CCNNNNNNNGG 3 cut(s) 158, 356, 655
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 139, 691
BseNI ACTGG 4 cut(s) 225, 483, 586, 761
BseSI GKGCMC 2 cut(s) 362, 761
BseXI GCAGC 3 cut(s) 160, 245, 248
BsgI GTGCAG 2 cut(s) 555, 593
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 63, 299, 759
BshNI GGYRCC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 159, 405
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 3 cut(s) 158, 356, 655
BsmAI GTCTC 2 cut(s) 413, 744
BsmFI GGGAC 1 cut(s) 63
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 3 cut(s) 63, 299, 759
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 197
Bsp120I GGGCCC 1 cut(s) 757
Bsp1286I GDGCHC 3 cut(s) 326, 362, 761
BspACI CCGC 6 cut(s) 58, 60, 69, 176, 239, 926
BspANI GGCC 3 cut(s) 63, 299, 759
BspCNI CTCAG 2 cut(s) 138, 690
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 325, 349, 361, 759
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 404
BsrI ACTGG 4 cut(s) 225, 483, 586, 761
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 4 cut(s) 58, 281, 300, 374
BssNAI GTATAC 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 300, 374
Bst1107I GTATAC 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 77, 282
Bst6I CTCTTC 3 cut(s) 240, 255, 264
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 2 cut(s) 125, 677
BstDSI CCRYGG 2 cut(s) 58, 281
BstF5I GGATG 1 cut(s) 778
BstFNI CGCG 1 cut(s) 60
BstMAI GTCTC 2 cut(s) 413, 744
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 2 cut(s) 362, 761
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 160, 245, 248
BstV2I GAAGAC 1 cut(s) 584
BstZ17I GTATAC 1 cut(s) 624
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 3 cut(s) 63, 299, 759
BtgI CCRYGG 2 cut(s) 58, 281
BtrI CACGTC 2 cut(s) 307, 655
BtsCI GGATG 1 cut(s) 778
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 347, 757, 758
Cfr42I CCGCGG 1 cut(s) 61
Csp6I GTAC 2 cut(s) 613, 746
CviAII CATG 1 cut(s) 592
CviQI GTAC 2 cut(s) 613, 746
DdeI CTNAG 2 cut(s) 125, 677
DrdI GACNNNNNNGTC 1 cut(s) 126
DseDI GACNNNNNNGTC 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 240, 255, 264
EarI CTCTTC 3 cut(s) 240, 255, 264
Eco130I CCWWGG 2 cut(s) 300, 374
Eco147I AGGCCT 1 cut(s) 299
Eco24I GRGCYC 2 cut(s) 326, 761
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 757
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 300, 374
EcoT38I GRGCYC 2 cut(s) 326, 761
ErhI CCWWGG 2 cut(s) 300, 374
FaeI CATG 1 cut(s) 595
FaiI YATR 9 cut(s) 102, 402, 465, 497, 502, 593, 624, 945, 968
FaqI GGGAC 1 cut(s) 63
FatI CATG 1 cut(s) 591
FauI CCCGC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 6 cut(s) 61, 69, 174, 177, 234, 237
FokI GGATG 1 cut(s) 785
FriOI GRGCYC 2 cut(s) 326, 761
Fsp4HI GCNGC 6 cut(s) 61, 69, 174, 177, 234, 237
GluI GCNGC 6 cut(s) 61, 69, 174, 177, 234, 237
HaeIII GGCC 3 cut(s) 63, 299, 759
HapII CCGG 2 cut(s) 159, 405
Hin1II CATG 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 8 cut(s) 216, 286, 340, 379, 701, 971, 1001, 1010
HpaII CCGG 2 cut(s) 159, 405
HphI GGTGA 2 cut(s) 388, 493
Hpy166II GTNNAC 2 cut(s) 318, 624
Hpy188I TCNGA 9 cut(s) 119, 132, 528, 680, 723, 853, 955, 1000, 1006
Hpy188III TCNNGA 5 cut(s) 290, 433, 735, 802, 860
Hpy8I GTNNAC 2 cut(s) 318, 624
Hpy99I CGWCG 1 cut(s) 114
HpyAV CCTTC 3 cut(s) 105, 175, 289
HpyCH4III ACNGT 2 cut(s) 77, 282
HpyCH4IV ACGT 2 cut(s) 306, 654
HpyCH4V TGCA 4 cut(s) 504, 536, 562, 574
HpyF3I CTNAG 2 cut(s) 125, 677
HpySE526I ACGT 2 cut(s) 306, 654
Hsp92II CATG 1 cut(s) 595
KspI CCGCGG 1 cut(s) 61
LmnI GCTCC 2 cut(s) 329, 887
Lsp1109I GCAGC 3 cut(s) 160, 245, 248
LweI GCATC 2 cut(s) 544, 913
MaeII ACGT 2 cut(s) 306, 654
MaeIII GTNAC 4 cut(s) 328, 910, 933, 1016
MboII GAAGA 9 cut(s) 21, 254, 257, 272, 281, 444, 589, 750, 932
MhlI GDGCHC 3 cut(s) 326, 362, 761
MmeI TCCRAC 3 cut(s) 155, 193, 687
MroXI GAANNNNTTC 2 cut(s) 522, 685
MseI TTAA 2 cut(s) 668, 887
MspA1I CMGCKG 2 cut(s) 60, 239
MspI CCGG 2 cut(s) 159, 405
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 60
NlaIII CATG 1 cut(s) 595
NlaIV GGNNCC 4 cut(s) 325, 349, 361, 759
NmuCI GTSAC 2 cut(s) 328, 1016
NspV TTCGAA 1 cut(s) 197
PceI AGGCCT 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PctI GAATGC 1 cut(s) 193
PdmI GAANNNNTTC 2 cut(s) 522, 685
PfeI GAWTC 8 cut(s) 216, 286, 340, 379, 701, 971, 1001, 1010
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 6 cut(s) 62, 70, 175, 178, 235, 238
PsiI TTATAA 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 325, 349, 361, 759
PspOMI GGGCCC 1 cut(s) 757
PspPI GGNCC 3 cut(s) 347, 757, 758
RsaI GTAC 2 cut(s) 614, 747
RsaNI GTAC 2 cut(s) 613, 746
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 2 cut(s) 668, 887
SatI GCNGC 6 cut(s) 61, 69, 174, 177, 234, 237
Sau96I GGNCC 3 cut(s) 347, 757, 758
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 3 cut(s) 326, 362, 761
SfaNI GCATC 2 cut(s) 544, 913
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 197
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 6 cut(s) 58, 60, 69, 176, 239, 926
SspI AATATT 1 cut(s) 903
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 300, 374
TaaI ACNGT 2 cut(s) 77, 282
TaiI ACGT 2 cut(s) 309, 657
TaqI TCGA 5 cut(s) 39, 197, 460, 696, 872
TatI WGTACW 1 cut(s) 612
TauI GCSGC 3 cut(s) 63, 71, 179
TfiI GAWTC 8 cut(s) 216, 286, 340, 379, 701, 971, 1001, 1010
Tru1I TTAA 2 cut(s) 668, 887
Tru9I TTAA 2 cut(s) 668, 887
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 2 cut(s) 328, 1016
TseI GCWGC 3 cut(s) 173, 233, 236
Tsp45I GTSAC 2 cut(s) 328, 1016
TspDTI ATGAA 4 cut(s) 555, 648, 741, 933
TspGWI ACGGA 3 cut(s) 199, 316, 952
TspRI CASTG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 5 cut(s) 199, 205, 600, 717, 729
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 2 cut(s) 522, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.