Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000784

Physical Location & Seq

Genomic Coordinates Forward (+)

606006 .. 609068

Gene Structure 3063 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1009400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGGGTGTTCAAGATTTCTGGGTTTTCATTTTTGTTATTGGGTTTTGCTTACAAGCTCGGGTTTCGAGCTCTCAAAACCTGACATGCAATCCAAACGATTTGAAGGCATTGGAGGATTTCATGAACGGTATAGATTCTGTGATTGATGGGTGGGGTAGCAATTTCTCACCTGATTGCTGCAAATGGGCAGGCATCACTTGCAATTCTTCTTCCTCTCTCGGATTGAACGATTCCATTGATACTTATAGAGTGGTTAAGTTGGAGCTTCCAAAGAGAAGGCTAGTGGGAAATCTCTCTGCATCTTTAGGCACTTTGGACCAGCTTAGAACCCTCAATCTCTCTCAAAATTTCCTCAAACACTCGCTTCCAATTTCGCTCTTCCATTTGCCGAATTTAGAGTTCTTGGACTTGAGCTCTAATGATTTTTCCGGCCCCATTCCTTTCGATATCGAATTGCCTTCAATCCAGTTCCTTGAAATTTCTCAAAACTTTTTGAATGGTACCCTTCCGGCTAGCATCTGTGACAAGTCTACTCAGCTTCGAGCACTGAAATTGGCTGTGAACTACTTCTCTGGTAACCTCCCACCAGGTCTTGGCAATTGTACTTCCTTGGAGGACCTCTGTCTAGGCATGAATAATTTCAATGGCGGTGTGCCCGAAGGTCTATTTCGTCTGCGAAACCTAACCCGGTTGAACATTCAAGATAACAAGCTTTCCGGGGTGCTGAGCGAAGAATTCGGCAACCTCACTAATCTTGTTTATTTGGATATCTCAACTAATGAATTTTCAGGAACCATCCCAGATGTTTTCCACAGCCTTGGAAGATTACAGAATCTTGTACTTCATTCAAATCGGTTCGGTGGTCGGATACCCCCTTCCTTGTCGAGTTCCTCGACCATCTCTTTGCTTAATTTGAGAAACAATTCATTGCGGGGCACAATTGATCTTAATTGTTCAGCAATGACTAGTTTGACCTCTCTTGATCTCGGTTCCAATCAGTTTGATGGGCCTATTCCCTCCAATCTTCCCTCTTGTCGACATTTGAATAATGTCAATCTTGCCCGTAACAACTTCACGGGCGAAATACCAGAAAGCTTCAAGAGTTTCCATAGCCTCTCTTACCTCTCGCTGTCAAACTCCAGCCTTTCCAATATCTCTTCTGCCTTACAAATTTTACAGCAGTGCCAGAACCTGACTACTTTGGTTCTCACCTTGAACTTCCGCGGCGAAGAATTTCCTGCTGATCCGACCCTTCGTTTTGAAAAGTTGAAGGTTCTTATTATTCCAAACTGTAGGCTCACAGGTGTAATACCTCAGTGGTTGAGTACTAGCAGCAGATTGCAATTGTTGGATATATCGTGGAACCAATTGCAAGGAACAATTCCAGTCTGGTTTGGCAATTTTAGCAGTCTTTTCTACTTGGACCTGTCGAATAATTCGTTTACGGGGGAAATCCCTAGAAGCTTAACTGGACTACCGAGCCTCATTAGTGGGAGGATCTCCATTGAGGAACCTTCCCCTGATTTCCCTCTGTTCATGAAGAGGAATGTAAGTGCACGAGGGTTGCAGTACAATCAAGTGTGGAGCTTTCCACCGACGCTGGAACTTAGTAACAATAATCTTAGCGGACCAATCTGGCCAGAGTTTGGGAAACTGAAATTGCTTCATGTTTTGGATCTGAAGTGCAACAATCTCTCAGGACCAATACCGAGTAATTTATCTGGTATGGCCTGCTTAGAGACTCTGGATTTGTCTCATAACAAGCTTTCAGGTACAATCCCGCCTTCGTTGGTTAAGCTTAGCTTCTTGTCCAAGTTTAGTGTTGCGGACAATCAGTTATACGGGGTGATCCCTACAGGAGGTCAGTTTGGGACCTTCCCAAATTCAAGCTTTGAAGGGAATAATCTTTGGGGCGACCATGCGCCCCGTTCATCAAAGGAGCATTATCCTCTTGGGAATCCCAGCAAATCAAGAAAAAATAGAGGTGTTATTGTTGGTATAGCTGTCGGAATTGTAAGTGGAACAGCACTTGTTCTTGCTCTCATGTTCACAATTTTGGTGCGGGCACATAGCCGGAGAGGGGTTGATCCTGAAAGAGAGGACCACGATACCAACGAGAAGGATTTGGAAGAATCCGGGTCAAAACTAGTGGTTCTGTTCCAAAACAAGGATGCCAATAAAGAGCTCTCTATTGATGACCTTCTACAATCTACCAACAATTTCGACCAAGCAAATATCATCGGTTGCGGGGGTTTTGGTTTGGTTTACAAGGCCAGCCTTCCTGATGGTAAGAAGGTTGCTATCAAGCGGCTTTCTGGTGACTGTGGCCAGATGGACAGAGAATTCCATGCTGAAGTTGAAACCTTGTCTAGAGCTCAGCATCCAAATCTTGTACATCTTCAGGGGTATTGTACGTATAAAAGTGACAGGCTCTTGATATATTCTTACATGGAGAATGGTAGTCTAGATTACTGGTTACATGAGAAAAGTGATGGTCCGACCTCTCTAGATTGGAATGTGAGGCTTCAGATTGCTCAAGGGGCGGCGAGAGGGCTTGCTTATTTGCACCAGTCATGCGAGCCCCACATCCTTCATCGGGATATAAAGTCAAGCAACATCCTTTTGGATGGGAATTTTAAAGCGCACTTAGCGGATTTTGGTCTTGCAAGGCTGATAGATCCGTATGATACTCATGTAACAACTGATCTTGTTGGGACATTAGGCTACATCCCTCCTGAGTACGGCCAAGCCTCTGTTGCTACTTACAAGGGCGATGTGTACAGTTTCGGGGTTGTTCTTTTGGAGCTGCTCACCGGAAGGAGGCCGATGGATATGTGCAAGCCAAAAGGAAGCCGGGATTTGATCTCGTGGGCCTTTCAGATGAAGAGGGAAAAGAGGGAAAGCGAGGTGTTTGATCCTTTCATTTGTGACAAGCAGCACGATGAGGAGCTGTTGTGTGTTTTTGAGATTGCATGCCTCTGTTTAAGCGGATGCCCGAAGGTGAGGCCTTCAACGCAGCAGCTAGTTTCGTGGCTCGAGAACATCAACACCAAGAAAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004674 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009975 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0031347 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0052652 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0072521 GO:0072522 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase

Protein Analysis

1021

Amino Acids

112.82

Weight (kDa)

6.03

Isoelectric Point (pI)

37.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 68	3.7e-06	Leucine rich repeat N-terminal domain
LRR_8	PF13855	100 - 142	1.3e-06	Leucine rich repeat
LRR_14	PF23598	100 - 330	1.7e-11	Leucine-rich repeat region
LRR_8	PF13855	226 - 286	1.7e-09	Leucine rich repeat
LRR_8	PF13855	422 - 480	8.1e-10	Leucine rich repeat
LRR_8	PF13855	533 - 589	8.3e-07	Leucine rich repeat
Pkinase	PF00069	743 - 1009	1.6e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	745 - 966	3.9e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02220
fragaria_vesca	FvH4_4g36380 FvH4_4g36410 FvH4_4g36411 FvH4_4g36420 FvH4_4g36420 FvH4_4g36601 FvH4_4g36601 FvH4_4g36601
malus_domestica	MD13G1009400.v1.1 MD13G1088300.v1.1 MD16G1007400.v1.1 MD16G1007500.v1.1 MD16G1007600.v1.1
prunus_persica	Prupe.1G343200_v2.0.a1 Prupe.1G343300_v2.0.a1
pyrus_communis	pycom13g00800 pycom16g00700 pycom16g00710 pycom16g00740 pycom16g00750
rosa_chinensis	RchiOBHm_Chr4g0420391 RchiOBHm_Chr4g0446131 RchiOBHm_Chr4g0446161 RchiOBHm_Chr4g0446181 RchiOBHm_Chr4g0446501
rosa_laevigata	RLG00000005654 RLG00000005685 RLG00000005686 RLG00000005687 RLG00000005689 RLG00000007768
rosa_multiflora	Rmu_co8048352.1_g000001 Rmu_sc0009254.1_g000002 Rmu_sc0009254.1_g000003 Rmu_sc0012061.1_g000004 Rmu_sc0024859.1_g000001 Rmu_sc0041209.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386530 Rroxscaffold_5G00386560 Rroxscaffold_5G00386570
rosa_rugosa	Rorug04G0163600 Rorug04G0368100 Rorug04G0368200 Rorug04G0368300 Rorug04G0372000
rosa_samantha	Rh4AG225100 Rh4AG429800 Rh4AG430000 Rh4AG430200 Rh4AG433600 Rh4BG439200 Rh4BG439500 Rh4BG439700 Rh4BG442700 Rh4CG239400 Rh4CG456500 Rh4CG456800 Rh4CG456900 Rh4CG460100 Rh4DG223500 Rh4DG437700 Rh4DG437900 Rh4DG438100 Rh4DG441400
rosa_wichuraiana	Rw4G019430 Rw4G036720 Rw4G036730 Rw4G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	500
AccB1I	GGYRCC	1 cut(s)	500
AccB7I	CCANNNNNTGG	2 cut(s)	593, 1646
AccI	GTMKAC	2 cut(s)	530, 1036
AccII	CGCG	1 cut(s)	1224
AclWI	GGATC	7 cut(s)	1238, 1505, 1683, 1843, 2081, 2672, 2909
AcoI	YGGCCR	3 cut(s)	1637, 2326, 2743
AcuI	CTGAAG	4 cut(s)	1700, 2373, 2384, 2510
AfiI	CCNNNNNNNGG	8 cut(s)	593, 1646, 1859, 2080, 2167, 2596, 2741, 2820
AhlI	ACTAGT	2 cut(s)	965, 2146
AjnI	CCWGG	1 cut(s)	586
Alw21I	GWGCWC	6 cut(s)	71, 416, 547, 1558, 2187, 2377
Alw26I	GTCTC	2 cut(s)	1733, 1758
Alw44I	GTGCAC	1 cut(s)	1554
AlwI	GGATC	7 cut(s)	1238, 1505, 1683, 1843, 2081, 2672, 2909
AlwNI	CAGNNNCTG	1 cut(s)	1192
Ama87I	CYCGRG	2 cut(s)	57, 3035
ApaLI	GTGCAC	1 cut(s)	1554
ApeKI	GCWGC	6 cut(s)	177, 1332, 2806, 2935, 3016, 3019
ArsI	GACNNNNNNTTYG	4 cut(s)	886, 918, 1732, 1764
Asp700I	GAANNNNTTC	4 cut(s)	566, 638, 922, 1233
Asp718I	GGTACC	1 cut(s)	500
AspLEI	GCGC	2 cut(s)	1924, 2644
AsuC2I	CCSGG	4 cut(s)	688, 718, 2137, 2855
AsuHPI	GGTGA	6 cut(s)	159, 1201, 1858, 2330, 2803, 3013
AsuNHI	GCTAGC	1 cut(s)	512
AvaI	CYCGRG	2 cut(s)	57, 3035
AvaII	GGWCC	8 cut(s)	316, 616, 1423, 1628, 1700, 1872, 2101, 2495
BaeGI	GKGCMC	4 cut(s)	657, 938, 1558, 2068
BaeI	ACNNNNGTAYC	2 cut(s)	2679, 2712
BalI	TGGCCA	2 cut(s)	1639, 2328
BanI	GGYRCC	1 cut(s)	500
BanII	GRGCYC	5 cut(s)	71, 416, 2187, 2377, 2583
BauI	CACGAG	2 cut(s)	1557, 2866
Bbv12I	GWGCWC	6 cut(s)	71, 416, 547, 1558, 2187, 2377
BbvI	GCAGC	6 cut(s)	164, 1344, 2793, 2947, 3028, 3031
BccI	CCATC	9 cut(s)	140, 803, 905, 998, 2279, 2326, 2486, 2621, 2821
BceAI	ACGGC	1 cut(s)	2758
BciT130I	CCWGG	1 cut(s)	588
BciVI	GTATCC	1 cut(s)	861
BcnI	CCSGG	4 cut(s)	688, 718, 2137, 2855
BcoDI	GTCTC	2 cut(s)	1733, 1758
BcuI	ACTAGT	2 cut(s)	965, 2146
BfmI	CTRYAG	2 cut(s)	1291, 1854
BfuI	GTATCC	1 cut(s)	861
BisI	GCNGC	9 cut(s)	178, 1225, 1333, 2309, 2544, 2807, 2936, 3017, 3020
BlpI	GCTNAGC	3 cut(s)	725, 1799, 2376
BlsI	GCNGC	9 cut(s)	179, 1226, 1334, 2310, 2545, 2808, 2937, 3018, 3021
BmcAI	AGTACT	1 cut(s)	1327
Bme1390I	CCNGG	5 cut(s)	588, 688, 718, 2137, 2855
Bme18I	GGWCC	8 cut(s)	316, 616, 1423, 1628, 1700, 1872, 2101, 2495
BmeT110I	CYCGRG	2 cut(s)	57, 3035
BmiI	GGNNCC	7 cut(s)	433, 502, 793, 991, 1364, 1512, 1873
BmrFI	CCNGG	5 cut(s)	588, 688, 718, 2137, 2855
BmsI	GCATC	6 cut(s)	201, 308, 525, 2161, 2389, 2981
BmtI	GCTAGC	1 cut(s)	516
BoxI	GACNNNNGTC	1 cut(s)	621
BpmI	CTGGAG	1 cut(s)	1123
Bpu1102I	GCTNAGC	3 cut(s)	725, 1799, 2376
BpuEI	CTTGAG	2 cut(s)	430, 2520
BpuMI	CCSGG	4 cut(s)	688, 718, 2137, 2855
BsaAI	YACGTR	1 cut(s)	2415
BsaJI	CCNNGG	4 cut(s)	609, 717, 817, 1222
BsaWI	WCCGGW	1 cut(s)	2813
BsaXI	ACNNNNNCTCC	2 cut(s)	2939, 2969
Bsc4I	CCNNNNNNNGG	8 cut(s)	593, 1646, 1859, 2080, 2167, 2596, 2741, 2820
Bse1I	ACTGG	5 cut(s)	466, 1385, 1474, 2477, 2569
Bse3DI	GCAATG	2 cut(s)	926, 966
BseBI	CCWGG	1 cut(s)	588
BseDI	CCNNGG	4 cut(s)	609, 717, 817, 1222
BseGI	GGATG	8 cut(s)	795, 2176, 2380, 2586, 2616, 2632, 2727, 2996
BseLI	CCNNNNNNNGG	8 cut(s)	593, 1646, 1859, 2080, 2167, 2596, 2741, 2820
BseMI	GCAATG	2 cut(s)	926, 966
BseMII	CTCAG	6 cut(s)	548, 716, 1328, 1710, 2390, 2727
BseNI	ACTGG	5 cut(s)	466, 1385, 1474, 2477, 2569
BseRI	GAGGAG	1 cut(s)	2960
BseSI	GKGCMC	4 cut(s)	657, 938, 1558, 2068
BseXI	GCAGC	6 cut(s)	164, 1344, 2793, 2947, 3028, 3031
BseYI	CCCAGC	1 cut(s)	1961
Bsh1236I	CGCG	1 cut(s)	1224
BshNI	GGYRCC	1 cut(s)	500
BsiHKAI	GWGCWC	6 cut(s)	71, 416, 547, 1558, 2187, 2377
BsiHKCI	CYCGRG	2 cut(s)	57, 3035
BsiSI	CCGG	8 cut(s)	429, 509, 688, 717, 2074, 2136, 2814, 2854
BslFI	GGGAC	2 cut(s)	1885, 2728
BslI	CCNNNNNNNGG	8 cut(s)	593, 1646, 1859, 2080, 2167, 2596, 2741, 2820
BsmAI	GTCTC	2 cut(s)	1733, 1758
BsmFI	GGGAC	2 cut(s)	1885, 2728
BsoBI	CYCGRG	2 cut(s)	57, 3035
Bsp1407I	TGTACA	2 cut(s)	2392, 2778
Bsp1720I	GCTNAGC	3 cut(s)	725, 1799, 2376
BspCNI	CTCAG	6 cut(s)	547, 717, 1327, 1709, 2389, 2728
BspFNI	CGCG	1 cut(s)	1224
BspHI	TCATGA	2 cut(s)	120, 1536
BspLI	GGNNCC	7 cut(s)	433, 502, 793, 991, 1364, 1512, 1873
BspOI	GCTAGC	1 cut(s)	516
BspPI	GGATC	7 cut(s)	1238, 1505, 1683, 1843, 2081, 2672, 2909
BspQI	GCTCTTC	1 cut(s)	383
BspT107I	GGYRCC	1 cut(s)	500
BsrDI	GCAATG	2 cut(s)	926, 966
BsrGI	TGTACA	2 cut(s)	2392, 2778
BsrI	ACTGG	5 cut(s)	466, 1385, 1474, 2477, 2569
BssECI	CCNNGG	4 cut(s)	609, 717, 817, 1222
BssSI	CACGAG	2 cut(s)	1557, 2866
BssT1I	CCWWGG	2 cut(s)	609, 817
Bst2BI	CACGAG	2 cut(s)	1557, 2866
Bst2UI	CCWGG	1 cut(s)	588
Bst4CI	ACNGT	4 cut(s)	128, 1292, 2324, 2783
Bst6I	CTCTTC	4 cut(s)	383, 1162, 1535, 2879
BstAPI	GCANNNNNTGC	1 cut(s)	198
BstAUI	TGTACA	2 cut(s)	2392, 2778
BstBAI	YACGTR	1 cut(s)	2415
BstC8I	GCNNGC	9 cut(s)	190, 514, 1732, 2064, 2275, 2556, 2579, 2840, 2974
BstDSI	CCRYGG	1 cut(s)	1222
BstEII	GGTNACC	1 cut(s)	575
BstENI	CCTNNNNNAGG	1 cut(s)	1857
BstF5I	GGATG	8 cut(s)	795, 2176, 2380, 2586, 2616, 2632, 2727, 2996
BstFNI	CGCG	1 cut(s)	1224
BstHHI	GCGC	2 cut(s)	1924, 2644
BstMAI	GTCTC	2 cut(s)	1733, 1758
BstMWI	GCNNNNNNNGC	6 cut(s)	198, 1404, 2540, 2648, 2756, 3013
BstNI	CCWGG	1 cut(s)	588
BstNSI	RCATGY	2 cut(s)	87, 2976
BstPAI	GACNNNNGTC	1 cut(s)	621
BstPI	GGTNACC	1 cut(s)	575
BstSCI	CCNGG	5 cut(s)	586, 686, 716, 2135, 2853
BstSFI	CTRYAG	2 cut(s)	1291, 1854
BstSLI	GKGCMC	4 cut(s)	657, 938, 1558, 2068
BstSNI	TACGTA	1 cut(s)	2415
BstUI	CGCG	1 cut(s)	1224
BstV1I	GCAGC	6 cut(s)	164, 1344, 2793, 2947, 3028, 3031
BstX2I	RGATCY	3 cut(s)	1497, 1675, 2677
BstXI	CCANNNNNNTGG	1 cut(s)	818
BstYI	RGATCY	3 cut(s)	1497, 1675, 2677
BsuI	GTATCC	1 cut(s)	861
BtgI	CCRYGG	1 cut(s)	1222
BtgZI	GCGATG	1 cut(s)	2787
BtsCI	GGATG	8 cut(s)	795, 2176, 2380, 2586, 2616, 2632, 2727, 2996
BtsI	GCAGTG	1 cut(s)	1187
BtsIMutI	CAGTG	3 cut(s)	545, 1187, 1322
Cac8I	GCNNGC	9 cut(s)	190, 514, 1732, 2064, 2275, 2556, 2579, 2840, 2974
CaiI	CAGNNNCTG	1 cut(s)	1192
CciI	TCATGA	2 cut(s)	120, 1536
CfoI	GCGC	2 cut(s)	1924, 2644
Cfr42I	CCGCGG	1 cut(s)	1225
CseI	GACGC	1 cut(s)	1606
CsiI	ACCWGGT	1 cut(s)	586
CspCI	CAANNNNNGTGG	2 cut(s)	2130, 2165
DraI	TTTAAA	1 cut(s)	2638
EaeI	YGGCCR	3 cut(s)	1637, 2326, 2743
Eam1104I	CTCTTC	4 cut(s)	383, 1162, 1535, 2879
EarI	CTCTTC	4 cut(s)	383, 1162, 1535, 2879
Ecl136II	GAGCTC	4 cut(s)	69, 414, 2185, 2375
Eco105I	TACGTA	1 cut(s)	2415
Eco130I	CCWWGG	2 cut(s)	609, 817
Eco147I	AGGCCT	1 cut(s)	3007
Eco24I	GRGCYC	5 cut(s)	71, 416, 2187, 2377, 2583
Eco32I	GATATC	2 cut(s)	448, 769
Eco47I	GGWCC	8 cut(s)	316, 616, 1423, 1628, 1700, 1872, 2101, 2495
Eco53kI	GAGCTC	4 cut(s)	69, 414, 2185, 2375
Eco57I	CTGAAG	4 cut(s)	1700, 2373, 2384, 2510
Eco88I	CYCGRG	2 cut(s)	57, 3035
Eco91I	GGTNACC	1 cut(s)	575
EcoICRI	GAGCTC	4 cut(s)	69, 414, 2185, 2375
EcoNI	CCTNNNNNAGG	1 cut(s)	1857
EcoO109I	RGGNCCY	2 cut(s)	616, 1872
EcoO65I	GGTNACC	1 cut(s)	575
EcoRI	GAATTC	2 cut(s)	734, 2342
EcoRII	CCWGG	1 cut(s)	586
EcoRV	GATATC	2 cut(s)	448, 769
EcoT14I	CCWWGG	2 cut(s)	609, 817
EcoT38I	GRGCYC	5 cut(s)	71, 416, 2187, 2377, 2583
ErhI	CCWWGG	2 cut(s)	609, 817
FalI	AAGNNNNNCTT	2 cut(s)	1787, 1819
FaqI	GGGAC	2 cut(s)	1885, 2728
FauI	CCCGC	4 cut(s)	924, 1788, 2055, 2240
FblI	GTMKAC	2 cut(s)	530, 1036
Fnu4HI	GCNGC	9 cut(s)	178, 1225, 1333, 2309, 2544, 2807, 2936, 3017, 3020
FokI	GGATG	8 cut(s)	782, 2183, 2367, 2573, 2603, 2639, 2714, 3003
FriOI	GRGCYC	5 cut(s)	71, 416, 2187, 2377, 2583
Fsp4HI	GCNGC	9 cut(s)	178, 1225, 1333, 2309, 2544, 2807, 2936, 3017, 3020
GlaI	GCGC	2 cut(s)	1923, 2643
GluI	GCNGC	9 cut(s)	178, 1225, 1333, 2309, 2544, 2807, 2936, 3017, 3020
GsaI	CCCAGC	1 cut(s)	1965
GsuI	CTGGAG	1 cut(s)	1123
HapII	CCGG	8 cut(s)	429, 509, 688, 717, 2074, 2136, 2814, 2854
HgaI	GACGC	1 cut(s)	1606
HhaI	GCGC	2 cut(s)	1924, 2644
Hin6I	GCGC	2 cut(s)	1922, 2642
HinP1I	GCGC	2 cut(s)	1922, 2642
HincII	GTYRAC	1 cut(s)	1037
HindII	GTYRAC	1 cut(s)	1037
HindIII	AAGCTT	6 cut(s)	710, 1093, 1462, 1763, 1796, 1888
HinfI	GANTC	6 cut(s)	134, 230, 832, 1741, 1957, 2132
HpaII	CCGG	8 cut(s)	429, 509, 688, 717, 2074, 2136, 2814, 2854
HphI	GGTGA	6 cut(s)	159, 1201, 1858, 2330, 2803, 3013
Hpy166II	GTNNAC	8 cut(s)	531, 562, 1037, 1443, 1556, 2049, 2266, 2779
Hpy188I	TCNGA	8 cut(s)	221, 867, 1248, 1680, 2009, 2499, 2529, 2880
Hpy8I	GTNNAC	8 cut(s)	531, 562, 1037, 1443, 1556, 2049, 2266, 2779
Hpy99I	CGWCG	1 cut(s)	1600
HpyCH4III	ACNGT	4 cut(s)	128, 1292, 2324, 2783
HpyCH4IV	ACGT	1 cut(s)	2414
HpyF10VI	GCNNNNNNNGC	6 cut(s)	198, 1404, 2540, 2648, 2756, 3013
HpySE526I	ACGT	1 cut(s)	2414
HspAI	GCGC	2 cut(s)	1922, 2642
KpnI	GGTACC	1 cut(s)	504
KspI	CCGCGG	1 cut(s)	1225
LguI	GCTCTTC	1 cut(s)	383
LmnI	GCTCC	5 cut(s)	262, 1584, 1939, 2803, 2947
Lsp1109I	GCAGC	6 cut(s)	164, 1344, 2793, 2947, 3028, 3031
LweI	GCATC	6 cut(s)	201, 308, 525, 2161, 2389, 2981
MabI	ACCWGGT	1 cut(s)	586
MaeII	ACGT	1 cut(s)	2414
MaeIII	GTNAC	9 cut(s)	521, 575, 1064, 1610, 2318, 2423, 2475, 2695, 2927
MfeI	CAATTG	4 cut(s)	598, 939, 1343, 1367
MflI	RGATCY	3 cut(s)	1497, 1675, 2677
MlsI	TGGCCA	2 cut(s)	1639, 2328
MluNI	TGGCCA	2 cut(s)	1639, 2328
MlyI	GAGTC	1 cut(s)	1735
MmeI	TCCRAC	6 cut(s)	240, 845, 1271, 1329, 1987, 2522
Mox20I	TGGCCA	2 cut(s)	1639, 2328
MroXI	GAANNNNTTC	4 cut(s)	566, 638, 922, 1233
MscI	TGGCCA	2 cut(s)	1639, 2328
MseI	TTAA	7 cut(s)	255, 909, 948, 1466, 1794, 2637, 2984
Msp20I	TGGCCA	2 cut(s)	1639, 2328
MspA1I	CMGCKG	1 cut(s)	1224
MspI	CCGG	8 cut(s)	429, 509, 688, 717, 2074, 2136, 2814, 2854
MspR9I	CCNGG	5 cut(s)	588, 688, 718, 2137, 2855
MunI	CAATTG	4 cut(s)	598, 939, 1343, 1367
MvaI	CCWGG	1 cut(s)	588
MvnI	CGCG	1 cut(s)	1224
MwoI	GCNNNNNNNGC	6 cut(s)	198, 1404, 2540, 2648, 2756, 3013
NciI	CCSGG	4 cut(s)	688, 718, 2137, 2855
NheI	GCTAGC	1 cut(s)	512
NlaIV	GGNNCC	7 cut(s)	433, 502, 793, 991, 1364, 1512, 1873
NmuCI	GTSAC	4 cut(s)	521, 2318, 2423, 2927
NspI	RCATGY	2 cut(s)	87, 2976
PaeI	GCATGC	1 cut(s)	2976
PaeR7I	CTCGAG	1 cut(s)	3035
PagI	TCATGA	2 cut(s)	120, 1536
PceI	AGGCCT	1 cut(s)	3007
PciSI	GCTCTTC	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	4 cut(s)	225, 890, 1436, 2112
PdmI	GAANNNNTTC	4 cut(s)	566, 638, 922, 1233
PfeI	GAWTC	5 cut(s)	134, 230, 832, 1957, 2132
PflMI	CCANNNNNTGG	2 cut(s)	593, 1646
PkrI	GCNGC	9 cut(s)	179, 1226, 1334, 2310, 2545, 2808, 2937, 3018, 3021
PleI	GAGTC	1 cut(s)	1735
PpsI	GAGTC	1 cut(s)	1735
Ppu21I	YACGTR	1 cut(s)	2415
PpuMI	RGGWCCY	2 cut(s)	616, 1872
PshAI	GACNNNNGTC	1 cut(s)	621
Psp124BI	GAGCTC	4 cut(s)	71, 416, 2187, 2377
Psp5II	RGGWCCY	2 cut(s)	616, 1872
Psp6I	CCWGG	1 cut(s)	586
PspEI	GGTNACC	1 cut(s)	575
PspFI	CCCAGC	1 cut(s)	1961
PspGI	CCWGG	1 cut(s)	586
PspN4I	GGNNCC	7 cut(s)	433, 502, 793, 991, 1364, 1512, 1873
PspPPI	RGGWCCY	2 cut(s)	616, 1872
PstNI	CAGNNNCTG	1 cut(s)	1192
PsuI	RGATCY	3 cut(s)	1497, 1675, 2677
SacI	GAGCTC	4 cut(s)	71, 416, 2187, 2377
SacII	CCGCGG	1 cut(s)	1225
SalI	GTCGAC	1 cut(s)	1035
SapI	GCTCTTC	1 cut(s)	383
SaqAI	TTAA	7 cut(s)	255, 909, 948, 1466, 1794, 2637, 2984
SatI	GCNGC	9 cut(s)	178, 1225, 1333, 2309, 2544, 2807, 2936, 3017, 3020
ScaI	AGTACT	1 cut(s)	1327
SchI	GAGTC	1 cut(s)	1735
ScrFI	CCNGG	5 cut(s)	588, 688, 718, 2137, 2855
SexAI	ACCWGGT	1 cut(s)	586
SfaNI	GCATC	6 cut(s)	201, 308, 525, 2161, 2389, 2981
SfcI	CTRYAG	2 cut(s)	1291, 1854
Sfr274I	CTCGAG	1 cut(s)	3035
Sfr303I	CCGCGG	1 cut(s)	1225
SgrBI	CCGCGG	1 cut(s)	1225
SinI	GGWCC	8 cut(s)	316, 616, 1423, 1628, 1700, 1872, 2101, 2495
SlaI	CTCGAG	1 cut(s)	3035
SmlI	CTYRAG	3 cut(s)	409, 2535, 3035
SmoI	CTYRAG	3 cut(s)	409, 2535, 3035
SnaBI	TACGTA	1 cut(s)	2415
SpeI	ACTAGT	2 cut(s)	965, 2146
SphI	GCATGC	1 cut(s)	2976
SseBI	AGGCCT	1 cut(s)	3007
SstI	GAGCTC	4 cut(s)	71, 416, 2187, 2377
StuI	AGGCCT	1 cut(s)	3007
StyD4I	CCNGG	5 cut(s)	586, 686, 716, 2135, 2853
StyI	CCWWGG	2 cut(s)	609, 817
TaaI	ACNGT	4 cut(s)	128, 1292, 2324, 2783
TaiI	ACGT	1 cut(s)	2417
TatI	WGTACW	6 cut(s)	602, 838, 1325, 1569, 2392, 2778
TauI	GCSGC	3 cut(s)	1227, 2311, 2546
TfiI	GAWTC	5 cut(s)	134, 230, 832, 1957, 2132
Tru1I	TTAA	7 cut(s)	255, 909, 948, 1466, 1794, 2637, 2984
Tru9I	TTAA	7 cut(s)	255, 909, 948, 1466, 1794, 2637, 2984
TscAI	CASTG	3 cut(s)	552, 1187, 1322
TseFI	GTSAC	4 cut(s)	521, 2318, 2423, 2927
TseI	GCWGC	6 cut(s)	177, 1332, 2806, 2935, 3016, 3019
Tsp45I	GTSAC	4 cut(s)	521, 2318, 2423, 2927
TspGWI	ACGGA	1 cut(s)	2670
TspRI	CASTG	3 cut(s)	552, 1187, 1322
Van91I	CCANNNNNTGG	2 cut(s)	593, 1646
VneI	GTGCAC	1 cut(s)	1554
VpaK11BI	GGWCC	8 cut(s)	316, 616, 1423, 1628, 1700, 1872, 2101, 2495
XagI	CCTNNNNNAGG	1 cut(s)	1857
XbaI	TCTAGA	3 cut(s)	2369, 2464, 2506
XceI	RCATGY	2 cut(s)	87, 2976
XhoI	CTCGAG	1 cut(s)	3035
XmiI	GTMKAC	2 cut(s)	530, 1036
XmnI	GAANNNNTTC	4 cut(s)	566, 638, 922, 1233
ZrmI	AGTACT	1 cut(s)	1327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.