Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0000784

Physical Location & Seq

Genomic Coordinates Reverse (-)

65413851 .. 65417510

Gene Structure 3660 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00386530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGGGTGTTCAAGATTTCTGGGTGGTCATCTTTGTTATTGGGTTTTGCTTCCAAGCTCAGATTTTGAGCTCTCAGAAGCTGACCTGCAATCTGAATGATTTGAATGCACTGGAGGAGTTCATGGGCGGTCTGAAAAGTGTCATTGGTGGTTGGGGCAAGAATATCTCTGATGATTGCTGTCAATGGGCAGGTATTACTTGCAACTCTTCATCCTCTCTTGGATTGAATGATTCTGTTGATACTTATAGAGTGGTTGGGTTGGATGTTTCGAGTAAAAAACTAATTGGGAATCTCTCTGAATCTTTGGGAAATTTGGACCAACTTAGAGCCCTCAATCTTTCTCACAACTTCCTTAAAAACTCACTTCCTGCCCAACTGTTCCAATTGCCAAATTTAGAGTACTTAGACTTGAGTTCTAATGACTTTTCTGGCCCCATTCCAGTTGATATTAATTTACCTTCACTTCTGTTCCTTGATATCTCTCAAAACTTCTTGAATGGTTCTATTCCACAAAGCATCTGTGCAAGTTCTACTAGGCTTCAGGTACTGAAGTTGGCTGTGAACTACTTGTCTGGTACTCTACCACCAAGTCTTGGAAATTGTAGTTCCTTGGAGGACCTCTGTCTCCTCACGAATAATCTCTCTGGTGGTGTACCTGAGGGTATATATCAGCTGCAAAATCTGACCAGATTGACTATTCAAGATAACAAGCTTACTGGGCCGCTGAGCAAAGAAGTTGGTAACCTGATTAACCTTAATCGTTTGGACATCTCAACGAATTGGTTTTCAGGGACTATTCCGGATGTTTTCCACAGCCTTGGAAGATTACAGTCTTTTGTTGCTCATTCCAATAATTTCAGTGGTCGGATACCTCCTTCCTTGTCAAGTTCCCCAAATATCTCTTTGCTTAATGTGAGAAACAATTCATTGGAGGGTCCAATTGATCTAAATTGTTCAGCAATGACAAGTCTGGCCTCTCTTGATCTAGGTTCAAACCAGTTTGATGGGGAAATTCCATCCAATCTTCCCTCTTGTCCACATTTGAATAATATAAATCTTGCCCGGAACAACTTGAGTGGCCCAATACCTGACAGCTTCAAGGATTTTCATACTCTGAATTACCTCTCACTTTCGAACTCCAGCCATTCTGATCTATCATCTGCCCTTCAAATTTTACAGCAGTGTCAGAATCTAACTACTTTGGTTCTCACCATGAACTTCTATGATGAAATATTGCCTGCTGATCCAACAATTCATTTTGTAAAGTTGAAGGTTTTCATTATTGCAAACAGTAGGCTCAAAGGTTCAATACCCCAGTGGTTGAGTAAGAGCAGCAGATTGCAGTTATTGGATATATCGTGGAACTGCTTGGAAGGAACAGTTCCAGCTTGGCTTGGCAATTTTACTAATCTCTTCTACTTGGACATATCAAACAATTCTTTTACTGGGGATATCCCGAGAAGCTTAACTGGACTACAGAGCCTCATTTATTGGAATGGTTCCAAGGTTCAGGAACCTTCCCCTGATTTTCCTCTTTTCCAGAAAAAGAATGTAAGTGCAAGGGGATTGCAGTACAATCAAGTTTGGAGCTTTCCACCGACGCTGGCACTTGGCAACAATTATTTGAGTGGACAGATCTGGGCAGAGTTTGGGAACCTGAAATCCCTTCATGTATTTGATTTAAAATTCAACAATTTATCTGGACCAATTCCAAGTTCTCTTGCTAATATGGCCAGCTTAGAGGCTCTGGATTTGTCTCATAACAAGCTTTCAGGAACAATACCCAGTTCACTGACTCGCCTCAGTTTTTTGTCCAAGTTCAGTGTTGCATACAATCAATTAGAGGGGGAGATTCCTAGTGGAGTTCAGTTTGGGACCTTCCCAAATTCAAGCTTTGAAGGAAATAATCTTTGTGGTGACCATGCTCCTCCATGTCCATCAAGGGGGAATACTTCTCCTGTTCAAACCAGAAAATCAAGAAAAAATGTTGCAAGCATTATTGGTATAGCTGTTGGAACTGTGTTTGGAACGGCAATTCTTCTTGCTCTCATGTTCATAATTGTTATGCGAGCACATAGTCGAAGAGAAGTTGATCCTGAAAAAGAGCACGATGCAGAAGAGAAGTATTTGGAAGAACTTGGGTCAAAATCAGTGGTTCTTTTCCATAACAAGGAGAACAATAAAGAACTCTCTCTTGACGACCTTCTGCAATCTACCAACAATTTTGATCAGGCAAATATCATTGGCTGCGGGGGTTTTGGTCTGGTTTACAGAGCCACCCTCCCTGATGGTAAGAAGGTTGCAATCAAGCGCTTGTCTGGTGACTGTGGCCAGATGGATAGAGAATTCCGTGCTGAAGTTGAAGCACTGTCAAGAGCTCAGCATCCAAATCTTGTCCATCTTCAAGGGTATTGCACATATAAAAGTGACAGGCTTTTGATATATTCTTACATGGAGAACAGTAGTCTCGACTATTGGTTACATGAAAAGCTGGACGGCGCATCCTGTCTAGATTGGAATACAAGGCTTCAAATTGCTCAAGGGGCGGCAAGAGGGCTTGCTTATTTGCATCAATCATGTGAGCCCCATATCGTTCATCGGGATATAAAGTCGAGTAATATTCTTTTAGATGAGAATTTTAAAGCCCACTTAGCTGATTTTGGTCTTGCAAGGCTCCTGCTTCCCTATGATACTCATGTAACAACTGATCTTGTTGGGACATTGGGCTACATTCCTCCTGAGTATGGCCAAGCCTCTGTTGCTACTTACAAGGGTGATGTATACAGTTTTGGGGTTGTTCTTTTGGAGCTTCTAACTGGAAAAAGGCCTATGGATATGTGCAAGCCAAAAGGAGCTCGGGATTTGATCTCTTGGGTGTTTCAGATGAAGAGGGAGAGGAGGGAAACTGAAGTGTTTGATCCAGTCATTTATGACAAGCAGAGAGATCAGGAGCTTTTGTGTGTTTTTGAGATTGCATTGCTTTGCTTAAGCGGATGCCCTAAGGTGAGACCTTCAACTCAGCAGCTAGTTTCTTGGCTCGATAATATCAACATCAAAATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004674 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009975 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0031347 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0052652 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0072521 GO:0072522 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1020

Amino Acids

112.63

Weight (kDa)

5.81

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	100 - 142	3.6e-06	Leucine rich repeat
LRR_14	PF23598	100 - 332	1.9e-13	Leucine-rich repeat region
LRR_4	PF12799	107 - 143	9.7e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	422 - 480	2.7e-06	Leucine rich repeat
LRR_8	PF13855	534 - 590	3.2e-07	Leucine rich repeat
Pkinase	PF00069	744 - 1010	7.2e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	746 - 966	5.4e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02220
fragaria_vesca	FvH4_4g36380 FvH4_4g36410 FvH4_4g36411 FvH4_4g36420 FvH4_4g36420 FvH4_4g36601 FvH4_4g36601 FvH4_4g36601
malus_domestica	MD13G1009400.v1.1 MD13G1088300.v1.1 MD16G1007400.v1.1 MD16G1007500.v1.1 MD16G1007600.v1.1
prunus_persica	Prupe.1G343200_v2.0.a1 Prupe.1G343300_v2.0.a1
pyrus_communis	pycom13g00800 pycom16g00700 pycom16g00710 pycom16g00740 pycom16g00750
rosa_chinensis	RchiOBHm_Chr4g0420391 RchiOBHm_Chr4g0446131 RchiOBHm_Chr4g0446161 RchiOBHm_Chr4g0446181 RchiOBHm_Chr4g0446501
rosa_laevigata	RLG00000005654 RLG00000005685 RLG00000005686 RLG00000005687 RLG00000005689 RLG00000007768
rosa_multiflora	Rmu_co8048352.1_g000001 Rmu_sc0009254.1_g000002 Rmu_sc0009254.1_g000003 Rmu_sc0012061.1_g000004 Rmu_sc0024859.1_g000001 Rmu_sc0041209.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386530 Rroxscaffold_5G00386560 Rroxscaffold_5G00386570
rosa_rugosa	Rorug04G0163600 Rorug04G0368100 Rorug04G0368200 Rorug04G0368300 Rorug04G0372000
rosa_samantha	Rh4AG225100 Rh4AG429800 Rh4AG430000 Rh4AG430200 Rh4AG433600 Rh4BG439200 Rh4BG439500 Rh4BG439700 Rh4BG442700 Rh4CG239400 Rh4CG456500 Rh4CG456800 Rh4CG456900 Rh4CG460100 Rh4DG223500 Rh4DG437700 Rh4DG437900 Rh4DG438100 Rh4DG441400
rosa_wichuraiana	Rw4G019430 Rw4G036720 Rw4G036730 Rw4G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	92, 179
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccI	GTMKAC	1 cut(s)	2781
AccIII	TCCGGA	1 cut(s)	799
AciI	CCGC	5 cut(s)	126, 722, 2250, 2546, 2991
AclWI	GGATC	3 cut(s)	1238, 2087, 2912
AcoI	YGGCCR	3 cut(s)	1731, 2329, 2746
AcsI	RAATTY	8 cut(s)	310, 391, 1011, 1170, 1685, 1885, 2345, 2635
AcuI	CTGAAG	4 cut(s)	524, 569, 2376, 2928
AfaI	GTAC	5 cut(s)	401, 546, 577, 654, 1574
AfeI	AGCGCT	1 cut(s)	2312
AfiI	CCNNNNNNNGG	4 cut(s)	593, 1943, 2170, 2744
AflII	CTTAAG	1 cut(s)	2986
AjuI	GAANNNNNNNTTGG	2 cut(s)	1699, 1731
AloI	GAACNNNNNNTCC	2 cut(s)	1646, 1678
Alw21I	GWGCWC	5 cut(s)	71, 2074, 2109, 2380, 2857
Alw26I	GTCTC	4 cut(s)	629, 1761, 2471, 3001
AlwI	GGATC	3 cut(s)	1238, 2087, 2912
AlwNI	CAGNNNCTG	2 cut(s)	79, 547
Ama87I	CYCGRG	2 cut(s)	1456, 2856
Aor13HI	TCCGGA	1 cut(s)	799
Aor51HI	AGCGCT	1 cut(s)	2312
AoxI	GGCC	8 cut(s)	430, 719, 972, 1078, 1731, 2329, 2746, 2825
ApeKI	GCWGC	4 cut(s)	673, 1332, 2247, 3022
ApoI	RAATTY	8 cut(s)	310, 391, 1011, 1170, 1685, 1885, 2345, 2635
AseI	ATTAAT	1 cut(s)	450
Asp700I	GAANNNNTTC	2 cut(s)	922, 1274
AspLEI	GCGC	2 cut(s)	2313, 2501
AspS9I	GGNCC	8 cut(s)	316, 431, 616, 719, 935, 1079, 1703, 1875
AsuC2I	CCSGG	1 cut(s)	1063
AsuHPI	GGTGA	5 cut(s)	1201, 1928, 2333, 2786, 3016
AsuII	TTCGAA	1 cut(s)	1133
AvaI	CYCGRG	2 cut(s)	1456, 2856
AvaII	GGWCC	5 cut(s)	316, 616, 935, 1703, 1875
AxyI	CCTNAGG	2 cut(s)	657, 3000
BaeI	ACNNNNGTAYC	4 cut(s)	536, 569, 2682, 2715
BalI	TGGCCA	3 cut(s)	1733, 2331, 2748
BanII	GRGCYC	5 cut(s)	71, 331, 2380, 2586, 2857
Bbv12I	GWGCWC	5 cut(s)	71, 2074, 2109, 2380, 2857
BbvI	GCAGC	4 cut(s)	660, 1344, 2234, 3034
BccI	CCATC	6 cut(s)	998, 1024, 1945, 2282, 2329, 2406
BceAI	ACGGC	2 cut(s)	2046, 2512
BciVI	GTATCC	1 cut(s)	861
BclI	TGATCA	1 cut(s)	2227
BcnI	CCSGG	1 cut(s)	1063
BcoDI	GTCTC	4 cut(s)	629, 1761, 2471, 3001
BfaI	CTAG	6 cut(s)	534, 986, 1857, 2510, 3026, 3061
BfmI	CTRYAG	1 cut(s)	1475
BfoI	RGCGCY	1 cut(s)	2314
BfrI	CTTAAG	1 cut(s)	2986
BfuAI	ACCTGC	2 cut(s)	92, 179
BfuI	GTATCC	1 cut(s)	861
BglII	AGATCT	1 cut(s)	1635
BisI	GCNGC	6 cut(s)	674, 722, 1333, 2248, 2547, 3023
BlpI	GCTNAGC	2 cut(s)	725, 2379
BlsI	GCNGC	6 cut(s)	675, 723, 1334, 2249, 2548, 3024
BmcAI	AGTACT	1 cut(s)	401
Bme1390I	CCNGG	1 cut(s)	1063
Bme18I	GGWCC	5 cut(s)	316, 616, 935, 1703, 1875
BmeT110I	CYCGRG	2 cut(s)	1456, 2856
BmgT120I	GGNCC	8 cut(s)	316, 431, 616, 719, 935, 1079, 1703, 1875
BmiI	GGNNCC	7 cut(s)	433, 936, 1501, 1515, 1655, 1876, 2675
BmrFI	CCNGG	1 cut(s)	1063
BmrI	ACTGGG	4 cut(s)	726, 1309, 1455, 1779
BmsI	GCATC	6 cut(s)	525, 2101, 2392, 2510, 2578, 2984
BmuI	ACTGGG	4 cut(s)	726, 1309, 1455, 1779
BoxI	GACNNNNGTC	1 cut(s)	621
BplI	GAGNNNNNCTC	2 cut(s)	2265, 2297
BpmI	CTGGAG	2 cut(s)	131, 1123
Bpu1102I	GCTNAGC	2 cut(s)	725, 2379
Bpu14I	TTCGAA	1 cut(s)	1133
BpuEI	CTTGAG	3 cut(s)	430, 1093, 2523
BpuMI	CCSGG	1 cut(s)	1063
BsaI	GGTCTC	1 cut(s)	3001
BsaJI	CCNNGG	3 cut(s)	609, 817, 1503
BsaWI	WCCGGW	1 cut(s)	799
BsaXI	ACNNNNNCTCC	2 cut(s)	2942, 2972
Bsc4I	CCNNNNNNNGG	4 cut(s)	593, 1943, 2170, 2744
Bse21I	CCTNAGG	2 cut(s)	657, 3000
Bse3DI	GCAATG	2 cut(s)	966, 2975
BseAI	TCCGGA	1 cut(s)	799
BseDI	CCNNGG	3 cut(s)	609, 817, 1503
BseGI	GGATG	7 cut(s)	209, 268, 808, 1016, 2383, 2501, 2999
BseLI	CCNNNNNNNGG	4 cut(s)	593, 1943, 2170, 2744
BseMI	GCAATG	2 cut(s)	966, 2975
BseMII	CTCAG	8 cut(s)	71, 86, 648, 716, 1816, 2393, 2730, 3032
BseRI	GAGGAG	4 cut(s)	128, 617, 1917, 2911
BseXI	GCAGC	4 cut(s)	660, 1344, 2234, 3034
BshFI	GGCC	8 cut(s)	432, 721, 974, 1080, 1733, 2331, 2748, 2827
BsiHKAI	GWGCWC	5 cut(s)	71, 2074, 2109, 2380, 2857
BsiHKCI	CYCGRG	2 cut(s)	1456, 2856
BsiSI	CCGG	2 cut(s)	800, 1063
BslFI	GGGAC	3 cut(s)	805, 1888, 2731
BslI	CCNNNNNNNGG	4 cut(s)	593, 1943, 2170, 2744
BsmAI	GTCTC	4 cut(s)	629, 1761, 2471, 3001
BsmFI	GGGAC	3 cut(s)	805, 1888, 2731
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	8 cut(s)	432, 721, 974, 1080, 1733, 2331, 2748, 2827
Bso31I	GGTCTC	1 cut(s)	3001
BsoBI	CYCGRG	2 cut(s)	1456, 2856
Bsp119I	TTCGAA	1 cut(s)	1133
Bsp1286I	GDGCHC	7 cut(s)	71, 331, 2074, 2109, 2380, 2586, 2857
Bsp13I	TCCGGA	1 cut(s)	799
Bsp1720I	GCTNAGC	2 cut(s)	725, 2379
BspACI	CCGC	5 cut(s)	126, 722, 2250, 2546, 2991
BspANI	GGCC	8 cut(s)	432, 721, 974, 1080, 1733, 2331, 2748, 2827
BspCNI	CTCAG	8 cut(s)	70, 85, 649, 717, 1815, 2392, 2731, 3031
BspEI	TCCGGA	1 cut(s)	799
BspLI	GGNNCC	7 cut(s)	433, 936, 1501, 1515, 1655, 1876, 2675
BspMI	ACCTGC	2 cut(s)	92, 179
BspPI	GGATC	3 cut(s)	1238, 2087, 2912
BspT104I	TTCGAA	1 cut(s)	1133
BspTI	CTTAAG	1 cut(s)	2986
BspTNI	GGTCTC	1 cut(s)	3001
BsrDI	GCAATG	2 cut(s)	966, 2975
BssECI	CCNNGG	3 cut(s)	609, 817, 1503
BssNAI	GTATAC	1 cut(s)	2782
BssT1I	CCWWGG	3 cut(s)	609, 817, 1503
Bst1107I	GTATAC	1 cut(s)	2782
Bst4CI	ACNGT	9 cut(s)	378, 831, 1292, 1381, 2020, 2327, 2370, 2462, 2786
Bst6I	CTCTTC	5 cut(s)	211, 1418, 2077, 2112, 2882
BstAFI	CTTAAG	1 cut(s)	2986
BstBI	TTCGAA	1 cut(s)	1133
BstC8I	GCNNGC	7 cut(s)	1239, 1605, 1735, 1993, 2070, 2559, 2843
BstEII	GGTNACC	2 cut(s)	740, 1916
BstF5I	GGATG	7 cut(s)	209, 268, 808, 1016, 2383, 2501, 2999
BstH2I	RGCGCY	1 cut(s)	2314
BstHHI	GCGC	2 cut(s)	2313, 2501
BstMAI	GTCTC	4 cut(s)	629, 1761, 2471, 3001
BstMWI	GCNNNNNNNGC	5 cut(s)	718, 1730, 2543, 2651, 2759
BstPAI	GACNNNNGTC	1 cut(s)	621
BstPI	GGTNACC	2 cut(s)	740, 1916
BstSCI	CCNGG	1 cut(s)	1061
BstSFI	CTRYAG	1 cut(s)	1475
BstV1I	GCAGC	4 cut(s)	660, 1344, 2234, 3034
BstX2I	RGATCY	1 cut(s)	1635
BstXI	CCANNNNNNTGG	2 cut(s)	818, 1004
BstYI	RGATCY	1 cut(s)	1635
BstZ17I	GTATAC	1 cut(s)	2782
Bsu36I	CCTNAGG	2 cut(s)	657, 3000
BsuI	GTATCC	1 cut(s)	861
BsuRI	GGCC	8 cut(s)	432, 721, 974, 1080, 1733, 2331, 2748, 2827
BtsCI	GGATG	7 cut(s)	209, 268, 808, 1016, 2383, 2501, 2999
BtsI	GCAGTG	1 cut(s)	1187
BtsIMutI	CAGTG	8 cut(s)	107, 865, 1187, 1322, 1790, 1828, 2157, 2366
BveI	ACCTGC	2 cut(s)	92, 179
Cac8I	GCNNGC	7 cut(s)	1239, 1605, 1735, 1993, 2070, 2559, 2843
CaiI	CAGNNNCTG	2 cut(s)	79, 547
CfoI	GCGC	2 cut(s)	2313, 2501
Cfr13I	GGNCC	8 cut(s)	316, 431, 616, 719, 935, 1079, 1703, 1875
CseI	GACGC	1 cut(s)	1609
Csp6I	GTAC	5 cut(s)	400, 545, 576, 653, 1573
CspCI	CAANNNNNGTGG	4 cut(s)	1057, 1092, 2133, 2168
CviQI	GTAC	5 cut(s)	400, 545, 576, 653, 1573
DraI	TTTAAA	2 cut(s)	1683, 2641
EaeI	YGGCCR	3 cut(s)	1731, 2329, 2746
Eam1104I	CTCTTC	5 cut(s)	211, 1418, 2077, 2112, 2882
EarI	CTCTTC	5 cut(s)	211, 1418, 2077, 2112, 2882
Ecl136II	GAGCTC	3 cut(s)	69, 2378, 2855
Eco130I	CCWWGG	3 cut(s)	609, 817, 1503
Eco147I	AGGCCT	1 cut(s)	2827
Eco24I	GRGCYC	5 cut(s)	71, 331, 2380, 2586, 2857
Eco31I	GGTCTC	1 cut(s)	3001
Eco32I	GATATC	2 cut(s)	478, 1453
Eco47I	GGWCC	5 cut(s)	316, 616, 935, 1703, 1875
Eco47III	AGCGCT	1 cut(s)	2312
Eco53kI	GAGCTC	3 cut(s)	69, 2378, 2855
Eco57I	CTGAAG	4 cut(s)	524, 569, 2376, 2928
Eco81I	CCTNAGG	2 cut(s)	657, 3000
Eco88I	CYCGRG	2 cut(s)	1456, 2856
Eco91I	GGTNACC	2 cut(s)	740, 1916
EcoICRI	GAGCTC	3 cut(s)	69, 2378, 2855
EcoO109I	RGGNCCY	2 cut(s)	616, 1875
EcoO65I	GGTNACC	2 cut(s)	740, 1916
EcoRI	GAATTC	1 cut(s)	2345
EcoRV	GATATC	2 cut(s)	478, 1453
EcoT14I	CCWWGG	3 cut(s)	609, 817, 1503
EcoT38I	GRGCYC	5 cut(s)	71, 331, 2380, 2586, 2857
ErhI	CCWWGG	3 cut(s)	609, 817, 1503
FaqI	GGGAC	3 cut(s)	805, 1888, 2731
FauI	CCCGC	1 cut(s)	2243
FbaI	TGATCA	1 cut(s)	2227
FblI	GTMKAC	1 cut(s)	2781
Fnu4HI	GCNGC	6 cut(s)	674, 722, 1333, 2248, 2547, 3023
FokI	GGATG	7 cut(s)	196, 275, 815, 1003, 2370, 2488, 3006
FriOI	GRGCYC	5 cut(s)	71, 331, 2380, 2586, 2857
Fsp4HI	GCNGC	6 cut(s)	674, 722, 1333, 2248, 2547, 3023
FspBI	CTAG	6 cut(s)	534, 986, 1857, 2510, 3026, 3061
GlaI	GCGC	2 cut(s)	2312, 2500
GluI	GCNGC	6 cut(s)	674, 722, 1333, 2248, 2547, 3023
GsuI	CTGGAG	2 cut(s)	131, 1123
HaeII	RGCGCY	1 cut(s)	2314
HaeIII	GGCC	8 cut(s)	432, 721, 974, 1080, 1733, 2331, 2748, 2827
HapII	CCGG	2 cut(s)	800, 1063
HgaI	GACGC	1 cut(s)	1609
HhaI	GCGC	2 cut(s)	2313, 2501
Hin6I	GCGC	2 cut(s)	2311, 2499
HinP1I	GCGC	2 cut(s)	2311, 2499
HindIII	AAGCTT	4 cut(s)	710, 1462, 1766, 1891
HinfI	GANTC	6 cut(s)	230, 289, 299, 1189, 1795, 1852
HpaII	CCGG	2 cut(s)	800, 1063
HphI	GGTGA	5 cut(s)	1201, 1928, 2333, 2786, 3016
Hpy166II	GTNNAC	7 cut(s)	562, 653, 1037, 1631, 1790, 2269, 2782
Hpy8I	GTNNAC	7 cut(s)	562, 653, 1037, 1631, 1790, 2269, 2782
Hpy99I	CGWCG	1 cut(s)	1603
HpyCH4III	ACNGT	9 cut(s)	378, 831, 1292, 1381, 2020, 2327, 2370, 2462, 2786
HpyF10VI	GCNNNNNNNGC	5 cut(s)	718, 1730, 2543, 2651, 2759
HspAI	GCGC	2 cut(s)	2311, 2499
Kpn2I	TCCGGA	1 cut(s)	799
Ksp22I	TGATCA	1 cut(s)	2227
LmnI	GCTCC	6 cut(s)	1587, 1930, 2679, 2806, 2852, 2950
Lsp1109I	GCAGC	4 cut(s)	660, 1344, 2234, 3034
LweI	GCATC	6 cut(s)	525, 2101, 2392, 2510, 2578, 2984
MaeI	CTAG	6 cut(s)	534, 986, 1857, 2510, 3026, 3061
MaeIII	GTNAC	6 cut(s)	740, 1916, 2321, 2426, 2478, 2698
MfeI	CAATTG	2 cut(s)	383, 939
MflI	RGATCY	1 cut(s)	1635
MhlI	GDGCHC	7 cut(s)	71, 331, 2074, 2109, 2380, 2586, 2857
MlsI	TGGCCA	3 cut(s)	1733, 2331, 2748
MluNI	TGGCCA	3 cut(s)	1733, 2331, 2748
MlyI	GAGTC	1 cut(s)	1789
MmeI	TCCRAC	4 cut(s)	240, 845, 1271, 1993
Mox20I	TGGCCA	3 cut(s)	1733, 2331, 2748
MroI	TCCGGA	1 cut(s)	799
MroXI	GAANNNNTTC	2 cut(s)	922, 1274
MscI	TGGCCA	3 cut(s)	1733, 2331, 2748
MseI	TTAA	9 cut(s)	354, 450, 750, 756, 909, 1466, 1682, 2640, 2987
Msp20I	TGGCCA	3 cut(s)	1733, 2331, 2748
MspA1I	CMGCKG	2 cut(s)	673, 724
MspCI	CTTAAG	1 cut(s)	2986
MspI	CCGG	2 cut(s)	800, 1063
MspR9I	CCNGG	1 cut(s)	1063
MunI	CAATTG	2 cut(s)	383, 939
Mva1269I	GAATGC	1 cut(s)	109
MwoI	GCNNNNNNNGC	5 cut(s)	718, 1730, 2543, 2651, 2759
NciI	CCSGG	1 cut(s)	1063
NlaIV	GGNNCC	7 cut(s)	433, 936, 1501, 1515, 1655, 1876, 2675
NmuCI	GTSAC	3 cut(s)	1916, 2321, 2426
NspV	TTCGAA	1 cut(s)	1133
PceI	AGGCCT	1 cut(s)	2827
PctI	GAATGC	1 cut(s)	109
PdmI	GAANNNNTTC	2 cut(s)	922, 1274
PfeI	GAWTC	5 cut(s)	230, 289, 299, 1189, 1852
PflMI	CCANNNNNTGG	1 cut(s)	593
PkrI	GCNGC	6 cut(s)	675, 723, 1334, 2249, 2548, 3024
PleI	GAGTC	1 cut(s)	1789
PpsI	GAGTC	1 cut(s)	1789
PpuMI	RGGWCCY	2 cut(s)	616, 1875
PshAI	GACNNNNGTC	1 cut(s)	621
PshBI	ATTAAT	1 cut(s)	450
Psp124BI	GAGCTC	3 cut(s)	71, 2380, 2857
Psp5II	RGGWCCY	2 cut(s)	616, 1875
PspEI	GGTNACC	2 cut(s)	740, 1916
PspN4I	GGNNCC	7 cut(s)	433, 936, 1501, 1515, 1655, 1876, 2675
PspPI	GGNCC	8 cut(s)	316, 431, 616, 719, 935, 1079, 1703, 1875
PspPPI	RGGWCCY	2 cut(s)	616, 1875
PstNI	CAGNNNCTG	2 cut(s)	79, 547
PsuI	RGATCY	1 cut(s)	1635
PvuII	CAGCTG	1 cut(s)	673
RsaI	GTAC	5 cut(s)	401, 546, 577, 654, 1574
RsaNI	GTAC	5 cut(s)	400, 545, 576, 653, 1573
SacI	GAGCTC	3 cut(s)	71, 2380, 2857
SaqAI	TTAA	9 cut(s)	354, 450, 750, 756, 909, 1466, 1682, 2640, 2987
SatI	GCNGC	6 cut(s)	674, 722, 1333, 2248, 2547, 3023
Sau96I	GGNCC	8 cut(s)	316, 431, 616, 719, 935, 1079, 1703, 1875
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	1 cut(s)	1789
ScrFI	CCNGG	1 cut(s)	1063
SduI	GDGCHC	7 cut(s)	71, 331, 2074, 2109, 2380, 2586, 2857
SfaNI	GCATC	6 cut(s)	525, 2101, 2392, 2510, 2578, 2984
SfcI	CTRYAG	1 cut(s)	1475
SfuI	TTCGAA	1 cut(s)	1133
SinI	GGWCC	5 cut(s)	316, 616, 935, 1703, 1875
SmlI	CTYRAG	4 cut(s)	409, 1072, 2538, 2986
SmoI	CTYRAG	4 cut(s)	409, 1072, 2538, 2986
SseBI	AGGCCT	1 cut(s)	2827
SsiI	CCGC	5 cut(s)	126, 722, 2250, 2546, 2991
SspI	AATATT	2 cut(s)	1233, 2620
SspMI	CTAG	6 cut(s)	534, 986, 1857, 2510, 3026, 3061
SstI	GAGCTC	3 cut(s)	71, 2380, 2857
StuI	AGGCCT	1 cut(s)	2827
StyD4I	CCNGG	1 cut(s)	1061
StyI	CCWWGG	3 cut(s)	609, 817, 1503
TaaI	ACNGT	9 cut(s)	378, 831, 1292, 1381, 2020, 2327, 2370, 2462, 2786
TaqI	TCGA	6 cut(s)	269, 1133, 2080, 2469, 2612, 3039
TatI	WGTACW	2 cut(s)	399, 1572
TauI	GCSGC	2 cut(s)	724, 2549
TfiI	GAWTC	5 cut(s)	230, 289, 299, 1189, 1852
Tru1I	TTAA	9 cut(s)	354, 450, 750, 756, 909, 1466, 1682, 2640, 2987
Tru9I	TTAA	9 cut(s)	354, 450, 750, 756, 909, 1466, 1682, 2640, 2987
TscAI	CASTG	8 cut(s)	114, 865, 1187, 1322, 1797, 1828, 2157, 2373
TseFI	GTSAC	3 cut(s)	1916, 2321, 2426
TseI	GCWGC	4 cut(s)	673, 1332, 2247, 3022
Tsp45I	GTSAC	3 cut(s)	1916, 2321, 2426
TspGWI	ACGGA	1 cut(s)	2339
TspRI	CASTG	8 cut(s)	114, 865, 1187, 1322, 1797, 1828, 2157, 2373
Van91I	CCANNNNNTGG	1 cut(s)	593
Vha464I	CTTAAG	1 cut(s)	2986
VpaK11BI	GGWCC	5 cut(s)	316, 616, 935, 1703, 1875
VspI	ATTAAT	1 cut(s)	450
XapI	RAATTY	8 cut(s)	310, 391, 1011, 1170, 1685, 1885, 2345, 2635
XbaI	TCTAGA	1 cut(s)	2509
XmiI	GTMKAC	1 cut(s)	2781
XmnI	GAANNNNTTC	2 cut(s)	922, 1274
XspI	CTAG	6 cut(s)	534, 986, 1857, 2510, 3026, 3061
ZrmI	AGTACT	1 cut(s)	401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.