Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000784

Physical Location & Seq

Genomic Coordinates Forward (+)

562802 .. 570894

Gene Structure 8093 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1007500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3417 bp

ATGGACAGGGTGCTGGGCTGTTTTTTGGACTGTGTGCTGGGCAATGTCCACTCCGACTCTCGTGCGTGTGTTGAGAACTTGACCGTGTGTTGCTGTGATATGGGTGCTCAGGATTTCTGGGTGGTGATTGTTATTGGCTTTTGCTTCCATGCTCAAATCTTGAGCTCTCAGAACTTGACATGCAATCCCAACGATTTCAAAGCTTTGGAGGATTTCATGAACGGTATAGATTCCGTGATTGAAGGGTGGGGCAACGATTTTTCACCTAATTGCTGTAAATGGGCAGGCATCACTTGCAATTCTTCGTCCTCTCTCGGATTGAGCTATTCCATTGATACTTATAGAGTGGTAAAACTGGAGCTTCCAAAGAGAAGGCTACTGGGAAATCTCTCTGCATCTTTAGGGACCTTGGACCAGCTCAGAACCCTCAATCTCTCTCACAATTTCCTCAAGCACTCGCTTCCAGTTTCGCTCTTCCATTTGCCAAATTTAGAGGTCTTAGACATGAGTTTTAATGACTTTTCGAGCCCCATTCCTGTCGATATCGATTTGCCTTCAATCCAGTTCCTTGACATTTCTCAGAACTTTTTGAATGGTTCCCTTCCTGGCAGCATCTGTGACAACAATTTTACTCAACTTCGGGTACTCAACGTGGCCGCTAACTGCTTCTCTGGCAATCTTCCACCAGGTTTTGGAAATTGTACTTCCTTGGAGGACCTTGACCTGAGTGCGAATTATCTTACAGGCACTGGTATATTTCGTCTGCGAAAGCTAACCCAGTTGAACATTCAAGATAACTTGCTATCAGGGGCGCTCAGCGAACAATTCGGTAAACTCATTAACCTTGTTCGTTTGGATATCTCAACTAATGGGTTTTCAGGAACCATCCCAGATGTTTTCTACAGCCTTGGAAGATTACGGAATTTTGTAGCTCATTCAAATCGTTTTGGTGGTCAGATACCCCCTTCTTTGTCGAGTTCATCGACTATCTCCTTGCTTAATTTGAGAAACAATTCGTTGCAGGGCACAATTGATCTTAATTGTTCAGCAATGACTGGTTTGACCTGGCTTGATCTTAGTTCCAATCGGTTTGATGGGCCTATTCCCTCCAATCTTCCCTCTTGTCGACATTTGAGTAATGTCAATCTTTCCCGTAACAAATTCACTGGCGGAATACCAGATAGCTTCAAGAGTTTCCATGGCCTCTCTTTCCTCTCACTGTCAAATTGCAGCCTTCCCAATATTTCTTCTGCCCTTCAAATTTTACAGCAGTGTCCGAACCTGACTACTTTGGTTCTCACCTTGAACTTCCGTGGCGAACAATTTCCTGCTGATCCAACCCTTCAGTTTGAAAAGCTGAAGGTTCTTATTATTGCAAACTGTAGGCTCACAGGTGTAATACCCCAATGGTTGAGTACTAGCAGCAGATTGCAGTTGTTGGATATATCGTGGAACCATTTGGAAGGAACAATTCCAGTCTGGTTTGGCAATTTTAGCAGTCTTTTCTACTTGGACATGTCTAATAATTCCTTTACGGGGGAAATCTCGAGAAGCTTAACTGGACTCCCGAGCCTCATTAATGGGAGGATCTCCATTGAGGAACCTTCCTATGATTTCCCTCTTTTCATGAAGTCGAATGTGAGTGCACTTGGGTTGCAGTACAATCAAGTTTGGAGCTTTCGGCCTACATTGGACTTTAGCAACAACAATCTCAGTGGACCAATCTGGCCGGACTTCAGGAAGCTGAGGTTGCTTCATGTTTTGGATTTGAAGTACAACAGATTATCAGGACCGATTCCGAGTAGTTTATCGGAGATGGTGAGCTTGGAGACCCTGGATTTGTCTCATAACGAGCTTTCGGGGATAATCCCACCTTCGTTGGTTAAGCTTAGCTTCCTGTCCAAGTTTAGTGTTGCAGACAATGAATTGTATGGGGTGACCCCTACAGGAGGTCAATTTTCGACCTTCTCAAGTTCAAGTTTTGAAGGGAACAATCTTTGCGGCTACGATGCTGCGCCTTGTCCATCCGGACGGGATGATCCTCTGGGAACATGGTTCGGCAAATCAAGCAAGGATTATACCGGAGTTATTGCTGGAGTGTACTCTTGTGGGTGTGTTGAGAACTTGCCTGCGTGTTGCTGCGATATGGATGCTCAGGATTTCTGGGTGGTGATTGTTATTGGCTTTTGCTTCCAAGTTCAAATCTTGAGCTCTCAGAACTTTACATGCAATCCAAATGATGTGAAAGCATTGGAGGATTTCACGTCAAGTTTAAAAACTGTGATCGACGGGTGGGGAAGCAGTTTCTCATCTGATTGCTGCAAATGGGAAGGTATCACTTGCAACTCTTCATTCTCTCTCGGAATTGGATTGAACAATTCAATCGATACCTTTCGAGTGGTTAAGTTGGAGCTTCCAAGTAAAAGACTAGCAGGCAATCTCTCTGCATCTTTAGGCACCTTGGACCAGCTTAGAACCCTTAATCTCTCTCAAAATTACCTCATAGGCACGCTTCCAACTGCACTCTTCCGTTTGCGAAATTTACAACTCTTAGACTTGAGCTTTAATGATTTTTCCGGCCCCATTCCTTTCGGTATTGATTTACCTTCAATCAAGTTCCTTGACATTTCTCAAAACCTTTTGAATGGTTCCCTTCCTGGCAGCATCTGTGACAACAATTCTACTCAACTTCGGAGAGGAGAAGGTGCACTGTTTGAAGTCTGGTTTGGCTTGTTCTTCCACTGCTTTTACGGGCTCACGGGTTCAATCCCCCAATGGTTGAGTACTAGCAGCAGATTGCAGTTGTTGGATATATCATGGAACCAATTGGAAGGAACAATTCCAGTCTGGTTTGGCAATTTTAGCAGTCTCTTCTACTTGGACATATCTAACAATTCTCTCAGCGGGGAAATCCCAAGAAGCTTAACTGGACTATGGAGCTTCATTAATGGGAGGAGCTCAACTGAAGAACCTTCCCCTGATTTCCCTCTTTTCGTGTGGAGGGTGAGTGGACGAGGCTTGCAATACAATCGGATATCAGGACCAATTCCGACTAGTTTATCTGAGATGGTGAGCTTGGAGACTCTGGATTTGTCTCATAACGAGCTTTCGGGGATAATCCCGCCTTCGTTGGTTGACCTTAGCTTCTTGTCCAAGTTTAGTGTTGCGTACAATGAATTGTATGGGGTGATCCCTACAGGAGGTCAATTTTTGACCTTCTCAAGTTCAAGCTTTGAAGGGAACAGTCTTTGCGGCTACGATGCTTCCCCTTGTCCTTCCGGAGAGGATGGTCCTCTGGGAACATGGTACGGCAAATCAAACAAGGATTCTACCGGAGTTATTGCCGGAGTGGGTGTCGGATTCATATTTGGAATAGCATGTTTCGTTGGAATAGATAGGTACGTCTGGACTTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004674 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009975 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0031347 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0052652 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0072521 GO:0072522 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase

Protein Analysis

1139

Amino Acids

124.63

Weight (kDa)

4.92

Isoelectric Point (pI)

34.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	63 - 100	9.9e-07	Leucine rich repeat N-terminal domain
LRR_8	PF13855	119 - 174	2.7e-06	Leucine rich repeat
LRR_4	PF12799	139 - 175	2.3e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	182 - 335	7.7e-08	Leucine-rich repeat region
LRR_8	PF13855	452 - 510	3e-09	Leucine rich repeat
LRR_8	PF13855	585 - 643	4.8e-06	Leucine rich repeat
LRRNT_2	PF08263	745 - 782	1.3e-06	Leucine rich repeat N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02220
fragaria_vesca	FvH4_4g36380 FvH4_4g36410 FvH4_4g36411 FvH4_4g36420 FvH4_4g36420 FvH4_4g36601 FvH4_4g36601 FvH4_4g36601
malus_domestica	MD13G1009400.v1.1 MD13G1088300.v1.1 MD16G1007400.v1.1 MD16G1007500.v1.1 MD16G1007600.v1.1
prunus_persica	Prupe.1G343200_v2.0.a1 Prupe.1G343300_v2.0.a1
pyrus_communis	pycom13g00800 pycom16g00700 pycom16g00710 pycom16g00740 pycom16g00750
rosa_chinensis	RchiOBHm_Chr4g0420391 RchiOBHm_Chr4g0446131 RchiOBHm_Chr4g0446161 RchiOBHm_Chr4g0446181 RchiOBHm_Chr4g0446501
rosa_laevigata	RLG00000005654 RLG00000005685 RLG00000005686 RLG00000005687 RLG00000005689 RLG00000007768
rosa_multiflora	Rmu_co8048352.1_g000001 Rmu_sc0009254.1_g000002 Rmu_sc0009254.1_g000003 Rmu_sc0012061.1_g000004 Rmu_sc0024859.1_g000001 Rmu_sc0041209.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386530 Rroxscaffold_5G00386560 Rroxscaffold_5G00386570
rosa_rugosa	Rorug04G0163600 Rorug04G0368100 Rorug04G0368200 Rorug04G0368300 Rorug04G0372000
rosa_samantha	Rh4AG225100 Rh4AG429800 Rh4AG430000 Rh4AG430200 Rh4AG433600 Rh4BG439200 Rh4BG439500 Rh4BG439700 Rh4BG442700 Rh4CG239400 Rh4CG456500 Rh4CG456800 Rh4CG456900 Rh4CG460100 Rh4DG223500 Rh4DG437700 Rh4DG437900 Rh4DG438100 Rh4DG441400
rosa_wichuraiana	Rw4G019430 Rw4G036720 Rw4G036730 Rw4G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2456
AccB7I	CCANNNNNTGG	1 cut(s)	692
AccI	GTMKAC	1 cut(s)	1126
AccIII	TCCGGA	2 cut(s)	2027, 3278
AciI	CCGC	6 cut(s)	657, 1170, 2001, 2904, 3122, 3252
AclWI	GGATC	4 cut(s)	1328, 1595, 2033, 3184
AcoI	YGGCCR	2 cut(s)	654, 1727
AcsI	RAATTY	5 cut(s)	487, 922, 1160, 1260, 2539
AcuI	CTGAAG	4 cut(s)	1328, 1379, 1720, 2985
AfiI	CCNNNNNNNGG	4 cut(s)	692, 1536, 1949, 3200
AflIII	ACRYGT	1 cut(s)	1515
AhlI	ACTAGT	1 cut(s)	3053
AjiI	CACGTC	1 cut(s)	2265
AjnI	CCWGG	5 cut(s)	604, 685, 1064, 1833, 2656
AjuI	GAANNNNNNNTTGG	2 cut(s)	2709, 2741
Alw21I	GWGCWC	6 cut(s)	109, 167, 1648, 2213, 2710, 2960
Alw26I	GTCTC	5 cut(s)	1823, 1848, 2873, 3074, 3099
Alw44I	GTGCAC	2 cut(s)	1644, 2706
AlwI	GGATC	4 cut(s)	1328, 1595, 2033, 3184
AlwNI	CAGNNNCTG	3 cut(s)	615, 749, 2667
Ama87I	CYCGRG	2 cut(s)	1546, 1567
Aor13HI	TCCGGA	2 cut(s)	2027, 3278
AoxI	GGCC	6 cut(s)	654, 1097, 1201, 1682, 1727, 2578
ApaLI	GTGCAC	2 cut(s)	1644, 2706
ApeKI	GCWGC	8 cut(s)	609, 1230, 1422, 2012, 2139, 2319, 2661, 2790
ApoI	RAATTY	5 cut(s)	487, 922, 1160, 1260, 2539
ArsI	GACNNNNNNTTYG	4 cut(s)	1822, 1854, 3073, 3105
AseI	ATTAAT	2 cut(s)	1578, 2946
Asp700I	GAANNNNTTC	2 cut(s)	1012, 1323
AspLEI	GCGC	2 cut(s)	814, 2017
AsuHPI	GGTGA	9 cut(s)	136, 255, 1291, 1831, 1948, 2182, 3016, 3082, 3199
AvaI	CYCGRG	2 cut(s)	1546, 1567
AvaII	GGWCC	8 cut(s)	405, 412, 715, 1718, 1790, 2464, 3041, 3290
BaeGI	GKGCMC	3 cut(s)	1028, 1648, 2710
BanI	GGYRCC	1 cut(s)	2456
BanII	GRGCYC	5 cut(s)	167, 530, 2213, 2757, 2960
BauI	CACGAG	1 cut(s)	60
Bbv12I	GWGCWC	6 cut(s)	109, 167, 1648, 2213, 2710, 2960
BbvCI	CCTCAGC	1 cut(s)	1745
BbvI	GCAGC	8 cut(s)	621, 1242, 1434, 1999, 2126, 2306, 2673, 2802
BccI	CCATC	6 cut(s)	893, 1088, 1810, 2032, 3061, 3281
BceAI	ACGGC	1 cut(s)	3325
BcgI	CGANNNNNNTGC	6 cut(s)	44, 78, 2132, 2166, 3011, 3045
BciT130I	CCWGG	5 cut(s)	606, 687, 1066, 1835, 2658
BcoDI	GTCTC	5 cut(s)	1823, 1848, 2873, 3074, 3099
BcuI	ACTAGT	1 cut(s)	3053
BfaI	CTAG	4 cut(s)	1419, 2429, 2787, 3054
BfmI	CTRYAG	4 cut(s)	901, 1381, 1944, 3195
BfoI	RGCGCY	1 cut(s)	815
BlpI	GCTNAGC	2 cut(s)	815, 1889
BmcAI	AGTACT	2 cut(s)	1417, 2785
Bme1390I	CCNGG	5 cut(s)	606, 687, 1066, 1835, 2658
Bme18I	GGWCC	8 cut(s)	405, 412, 715, 1718, 1790, 2464, 3041, 3290
BmeT110I	CYCGRG	2 cut(s)	1546, 1567
BmgBI	CACGTC	1 cut(s)	2265
BmiI	GGNNCC	9 cut(s)	406, 598, 883, 1454, 1602, 2458, 2581, 2650, 2822
BmrFI	CCNGG	5 cut(s)	606, 687, 1066, 1835, 2658
BmrI	ACTGGG	2 cut(s)	389, 772
BmsI	GCATC	8 cut(s)	297, 404, 621, 1999, 2140, 2456, 2673, 3250
BmuI	ACTGGG	2 cut(s)	389, 772
BpmI	CTGGAG	2 cut(s)	377, 2115
Bpu10I	CCTNAGC	4 cut(s)	108, 1745, 2154, 3140
Bpu1102I	GCTNAGC	2 cut(s)	815, 1889
BpuEI	CTTGAG	6 cut(s)	181, 434, 1954, 2227, 2578, 3205
Bsa29I	ATCGAT	2 cut(s)	546, 2385
BsaI	GGTCTC	1 cut(s)	1823
BsaJI	CCNNGG	7 cut(s)	408, 708, 907, 1198, 1312, 1833, 2460
BsaWI	WCCGGW	4 cut(s)	2027, 2081, 3278, 3332
BsaXI	ACNNNNNCTCC	4 cut(s)	2992, 3022, 3339, 3369
Bsc4I	CCNNNNNNNGG	4 cut(s)	692, 1536, 1949, 3200
Bse3DI	GCAATG	2 cut(s)	49, 1056
BseAI	TCCGGA	2 cut(s)	2027, 3278
BseBI	CCWGG	5 cut(s)	606, 687, 1066, 1835, 2658
BseCI	ATCGAT	2 cut(s)	546, 2385
BseDI	CCNNGG	7 cut(s)	408, 708, 907, 1198, 1312, 1833, 2460
BseGI	GGATG	5 cut(s)	885, 2024, 2041, 2155, 3292
BseLI	CCNNNNNNNGG	4 cut(s)	692, 1536, 1949, 3200
BseMI	GCAATG	2 cut(s)	49, 1056
BseRI	GAGGAG	2 cut(s)	2712, 2968
BseSI	GKGCMC	3 cut(s)	1028, 1648, 2710
BseXI	GCAGC	8 cut(s)	621, 1242, 1434, 1999, 2126, 2306, 2673, 2802
BseYI	CCCAGC	2 cut(s)	13, 37
BsgI	GTGCAG	1 cut(s)	2505
BshFI	GGCC	6 cut(s)	656, 1099, 1203, 1684, 1729, 2580
BshNI	GGYRCC	1 cut(s)	2456
BshVI	ATCGAT	2 cut(s)	546, 2385
BsiHKAI	GWGCWC	6 cut(s)	109, 167, 1648, 2213, 2710, 2960
BsiHKCI	CYCGRG	2 cut(s)	1546, 1567
BsiSI	CCGG	7 cut(s)	1730, 2028, 2082, 2577, 3279, 3333, 3345
BslFI	GGGAC	1 cut(s)	418
BslI	CCNNNNNNNGG	4 cut(s)	692, 1536, 1949, 3200
BsmAI	GTCTC	5 cut(s)	1823, 1848, 2873, 3074, 3099
BsmFI	GGGAC	1 cut(s)	418
BsnI	GGCC	6 cut(s)	656, 1099, 1203, 1684, 1729, 2580
Bso31I	GGTCTC	1 cut(s)	1823
BsoBI	CYCGRG	2 cut(s)	1546, 1567
Bsp1286I	GDGCHC	9 cut(s)	109, 167, 530, 1028, 1648, 2213, 2710, 2757, 2960
Bsp13I	TCCGGA	2 cut(s)	2027, 3278
Bsp143I	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
Bsp1720I	GCTNAGC	2 cut(s)	815, 1889
Bsp19I	CCATGG	1 cut(s)	1198
BspACI	CCGC	6 cut(s)	657, 1170, 2001, 2904, 3122, 3252
BspANI	GGCC	6 cut(s)	656, 1099, 1203, 1684, 1729, 2580
BspDI	ATCGAT	2 cut(s)	546, 2385
BspEI	TCCGGA	2 cut(s)	2027, 3278
BspHI	TCATGA	2 cut(s)	216, 1626
BspLI	GGNNCC	9 cut(s)	406, 598, 883, 1454, 1602, 2458, 2581, 2650, 2822
BspPI	GGATC	4 cut(s)	1328, 1595, 2033, 3184
BspQI	GCTCTTC	1 cut(s)	479
BspT107I	GGYRCC	1 cut(s)	2456
BspTNI	GGTCTC	1 cut(s)	1823
BsrDI	GCAATG	2 cut(s)	49, 1056
BssECI	CCNNGG	7 cut(s)	408, 708, 907, 1198, 1312, 1833, 2460
BssMI	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
BssSI	CACGAG	1 cut(s)	60
BssT1I	CCWWGG	5 cut(s)	408, 708, 907, 1198, 2460
Bst2BI	CACGAG	1 cut(s)	60
Bst2UI	CCWGG	5 cut(s)	606, 687, 1066, 1835, 2658
Bst4CI	ACNGT	8 cut(s)	32, 85, 224, 1221, 1382, 2281, 2712, 3245
Bst6I	CTCTTC	4 cut(s)	479, 2353, 2531, 2876
BstAPI	GCANNNNNTGC	1 cut(s)	294
BstC8I	GCNNGC	5 cut(s)	286, 2130, 2434, 2510, 3020
BstDSI	CCRYGG	2 cut(s)	1198, 1312
BstEII	GGTNACC	1 cut(s)	1936
BstENI	CCTNNNNNAGG	2 cut(s)	1947, 3198
BstF5I	GGATG	5 cut(s)	885, 2024, 2041, 2155, 3292
BstH2I	RGCGCY	1 cut(s)	815
BstHHI	GCGC	2 cut(s)	814, 2017
BstKTI	GATC	7 cut(s)	1036, 1075, 1336, 1590, 2041, 2286, 3192
BstMAI	GTCTC	5 cut(s)	1823, 1848, 2873, 3074, 3099
BstMBI	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
BstMWI	GCNNNNNNNGC	6 cut(s)	294, 672, 1494, 1750, 2067, 2862
BstNI	CCWGG	5 cut(s)	606, 687, 1066, 1835, 2658
BstNSI	RCATGY	4 cut(s)	183, 1519, 2229, 3381
BstPI	GGTNACC	1 cut(s)	1936
BstSCI	CCNGG	5 cut(s)	604, 685, 1064, 1833, 2656
BstSFI	CTRYAG	4 cut(s)	901, 1381, 1944, 3195
BstSLI	GKGCMC	3 cut(s)	1028, 1648, 2710
BstV1I	GCAGC	8 cut(s)	621, 1242, 1434, 1999, 2126, 2306, 2673, 2802
BstX2I	RGATCY	1 cut(s)	1587
BstYI	RGATCY	1 cut(s)	1587
Bsu15I	ATCGAT	2 cut(s)	546, 2385
BsuRI	GGCC	6 cut(s)	656, 1099, 1203, 1684, 1729, 2580
BsuTUI	ATCGAT	2 cut(s)	546, 2385
BtgI	CCRYGG	2 cut(s)	1198, 1312
BtrI	CACGTC	1 cut(s)	2265
BtsCI	GGATG	5 cut(s)	885, 2024, 2041, 2155, 3292
BtsI	GCAGTG	2 cut(s)	1277, 2740
BtsIMutI	CAGTG	7 cut(s)	747, 1164, 1217, 1277, 1720, 2708, 2740
Cac8I	GCNNGC	5 cut(s)	286, 2130, 2434, 2510, 3020
CaiI	CAGNNNCTG	3 cut(s)	615, 749, 2667
CciI	TCATGA	2 cut(s)	216, 1626
CfoI	GCGC	2 cut(s)	814, 2017
ClaI	ATCGAT	2 cut(s)	546, 2385
CsiI	ACCWGGT	1 cut(s)	685
CspCI	CAANNNNNGTGG	4 cut(s)	1696, 1731, 1860, 1895
DpnI	GATC	7 cut(s)	1035, 1074, 1335, 1589, 2040, 2285, 3191
DpnII	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
DraI	TTTAAA	1 cut(s)	2274
EaeI	YGGCCR	2 cut(s)	654, 1727
Eam1104I	CTCTTC	4 cut(s)	479, 2353, 2531, 2876
EarI	CTCTTC	4 cut(s)	479, 2353, 2531, 2876
EciI	GGCGGA	1 cut(s)	1185
Ecl136II	GAGCTC	3 cut(s)	165, 2211, 2958
Eco130I	CCWWGG	5 cut(s)	408, 708, 907, 1198, 2460
Eco24I	GRGCYC	5 cut(s)	167, 530, 2213, 2757, 2960
Eco31I	GGTCTC	1 cut(s)	1823
Eco32I	GATATC	3 cut(s)	544, 859, 3036
Eco47I	GGWCC	8 cut(s)	405, 412, 715, 1718, 1790, 2464, 3041, 3290
Eco53kI	GAGCTC	3 cut(s)	165, 2211, 2958
Eco57I	CTGAAG	4 cut(s)	1328, 1379, 1720, 2985
Eco88I	CYCGRG	2 cut(s)	1546, 1567
Eco91I	GGTNACC	1 cut(s)	1936
EcoICRI	GAGCTC	3 cut(s)	165, 2211, 2958
EcoNI	CCTNNNNNAGG	2 cut(s)	1947, 3198
EcoO109I	RGGNCCY	2 cut(s)	405, 715
EcoO65I	GGTNACC	1 cut(s)	1936
EcoRII	CCWGG	5 cut(s)	604, 685, 1064, 1833, 2656
EcoRV	GATATC	3 cut(s)	544, 859, 3036
EcoT14I	CCWWGG	5 cut(s)	408, 708, 907, 1198, 2460
EcoT38I	GRGCYC	5 cut(s)	167, 530, 2213, 2757, 2960
ErhI	CCWWGG	5 cut(s)	408, 708, 907, 1198, 2460
FalI	AAGNNNNNCTT	2 cut(s)	1877, 1909
FaqI	GGGAC	1 cut(s)	418
FauI	CCCGC	2 cut(s)	2897, 3129
FblI	GTMKAC	1 cut(s)	1126
FokI	GGATG	5 cut(s)	872, 2011, 2048, 2162, 3299
FriOI	GRGCYC	5 cut(s)	167, 530, 2213, 2757, 2960
FspBI	CTAG	4 cut(s)	1419, 2429, 2787, 3054
GlaI	GCGC	2 cut(s)	813, 2016
GsaI	CCCAGC	2 cut(s)	17, 41
GsuI	CTGGAG	2 cut(s)	377, 2115
HaeII	RGCGCY	1 cut(s)	815
HaeIII	GGCC	6 cut(s)	656, 1099, 1203, 1684, 1729, 2580
HapII	CCGG	7 cut(s)	1730, 2028, 2082, 2577, 3279, 3333, 3345
HhaI	GCGC	2 cut(s)	814, 2017
Hin6I	GCGC	2 cut(s)	812, 2015
HinP1I	GCGC	2 cut(s)	812, 2015
HincII	GTYRAC	2 cut(s)	1127, 3136
HindII	GTYRAC	2 cut(s)	1127, 3136
HindIII	AAGCTT	5 cut(s)	201, 1552, 1886, 2920, 3229
HinfI	GANTC	7 cut(s)	56, 230, 1563, 1795, 3082, 3326, 3360
HpaII	CCGG	7 cut(s)	1730, 2028, 2082, 2577, 3279, 3333, 3345
HphI	GGTGA	9 cut(s)	136, 255, 1291, 1831, 1948, 2182, 3016, 3082, 3199
Hpy166II	GTNNAC	9 cut(s)	49, 833, 1127, 1646, 1718, 2101, 2708, 3011, 3136
Hpy8I	GTNNAC	9 cut(s)	49, 833, 1127, 1646, 1718, 2101, 2708, 3011, 3136
Hpy99I	CGWCG	1 cut(s)	2291
HpyCH4III	ACNGT	8 cut(s)	32, 85, 224, 1221, 1382, 2281, 2712, 3245
HpyCH4IV	ACGT	3 cut(s)	651, 2264, 3402
HpyF10VI	GCNNNNNNNGC	6 cut(s)	294, 672, 1494, 1750, 2067, 2862
HpySE526I	ACGT	3 cut(s)	651, 2264, 3402
HspAI	GCGC	2 cut(s)	812, 2015
Kpn2I	TCCGGA	2 cut(s)	2027, 3278
Kzo9I	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
LguI	GCTCTTC	1 cut(s)	479
LmnI	GCTCC	5 cut(s)	358, 1674, 2410, 2937, 2955
Lsp1109I	GCAGC	8 cut(s)	621, 1242, 1434, 1999, 2126, 2306, 2673, 2802
LweI	GCATC	8 cut(s)	297, 404, 621, 1999, 2140, 2456, 2673, 3250
MabI	ACCWGGT	1 cut(s)	685
MaeI	CTAG	4 cut(s)	1419, 2429, 2787, 3054
MaeII	ACGT	3 cut(s)	651, 2264, 3402
MaeIII	GTNAC	4 cut(s)	617, 1154, 1936, 2669
MalI	GATC	7 cut(s)	1035, 1074, 1335, 1589, 2040, 2285, 3191
MboI	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
MfeI	CAATTG	2 cut(s)	1029, 2825
MflI	RGATCY	1 cut(s)	1587
MhlI	GDGCHC	9 cut(s)	109, 167, 530, 1028, 1648, 2213, 2710, 2757, 2960
MlyI	GAGTC	3 cut(s)	50, 1557, 3076
MmeI	TCCRAC	9 cut(s)	78, 1361, 1419, 2388, 2540, 2787, 3074, 3337, 3367
MroI	TCCGGA	2 cut(s)	2027, 3278
MroXI	GAANNNNTTC	2 cut(s)	1012, 1323
MspA1I	CMGCKG	1 cut(s)	2904
MspI	CCGG	7 cut(s)	1730, 2028, 2082, 2577, 3279, 3333, 3345
MspR9I	CCNGG	5 cut(s)	606, 687, 1066, 1835, 2658
MunI	CAATTG	2 cut(s)	1029, 2825
MvaI	CCWGG	5 cut(s)	606, 687, 1066, 1835, 2658
MwoI	GCNNNNNNNGC	6 cut(s)	294, 672, 1494, 1750, 2067, 2862
NcoI	CCATGG	1 cut(s)	1198
NdeII	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
NlaIV	GGNNCC	9 cut(s)	406, 598, 883, 1454, 1602, 2458, 2581, 2650, 2822
NmuCI	GTSAC	3 cut(s)	617, 1936, 2669
NspI	RCATGY	4 cut(s)	183, 1519, 2229, 3381
PaeR7I	CTCGAG	1 cut(s)	1546
PagI	TCATGA	2 cut(s)	216, 1626
PciI	ACATGT	1 cut(s)	1515
PciSI	GCTCTTC	1 cut(s)	479
PcsI	WCGNNNNNNNCGW	1 cut(s)	980
PdmI	GAANNNNTTC	2 cut(s)	1012, 1323
PfeI	GAWTC	4 cut(s)	230, 1795, 3326, 3360
PflMI	CCANNNNNTGG	1 cut(s)	692
PleI	GAGTC	3 cut(s)	50, 1557, 3076
PpsI	GAGTC	3 cut(s)	50, 1557, 3076
PpuMI	RGGWCCY	2 cut(s)	405, 715
PscI	ACATGT	1 cut(s)	1515
PshBI	ATTAAT	2 cut(s)	1578, 2946
Psp124BI	GAGCTC	3 cut(s)	167, 2213, 2960
Psp5II	RGGWCCY	2 cut(s)	405, 715
Psp6I	CCWGG	5 cut(s)	604, 685, 1064, 1833, 2656
PspEI	GGTNACC	1 cut(s)	1936
PspFI	CCCAGC	2 cut(s)	13, 37
PspGI	CCWGG	5 cut(s)	604, 685, 1064, 1833, 2656
PspN4I	GGNNCC	9 cut(s)	406, 598, 883, 1454, 1602, 2458, 2581, 2650, 2822
PspPPI	RGGWCCY	2 cut(s)	405, 715
PstNI	CAGNNNCTG	3 cut(s)	615, 749, 2667
PsuI	RGATCY	1 cut(s)	1587
SacI	GAGCTC	3 cut(s)	167, 2213, 2960
SalI	GTCGAC	1 cut(s)	1125
SapI	GCTCTTC	1 cut(s)	479
Sau3AI	GATC	7 cut(s)	1033, 1072, 1333, 1587, 2038, 2283, 3189
ScaI	AGTACT	2 cut(s)	1417, 2785
SchI	GAGTC	3 cut(s)	50, 1557, 3076
ScrFI	CCNGG	5 cut(s)	606, 687, 1066, 1835, 2658
SduI	GDGCHC	9 cut(s)	109, 167, 530, 1028, 1648, 2213, 2710, 2757, 2960
SexAI	ACCWGGT	1 cut(s)	685
SfaNI	GCATC	8 cut(s)	297, 404, 621, 1999, 2140, 2456, 2673, 3250
SfcI	CTRYAG	4 cut(s)	901, 1381, 1944, 3195
Sfr274I	CTCGAG	1 cut(s)	1546
SinI	GGWCC	8 cut(s)	405, 412, 715, 1718, 1790, 2464, 3041, 3290
SlaI	CTCGAG	1 cut(s)	1546
SmlI	CTYRAG	7 cut(s)	160, 449, 1546, 1969, 2206, 2557, 3220
SmoI	CTYRAG	7 cut(s)	160, 449, 1546, 1969, 2206, 2557, 3220
SpeI	ACTAGT	1 cut(s)	3053
SsiI	CCGC	6 cut(s)	657, 1170, 2001, 2904, 3122, 3252
SspI	AATATT	1 cut(s)	1243
SspMI	CTAG	4 cut(s)	1419, 2429, 2787, 3054
SstI	GAGCTC	3 cut(s)	167, 2213, 2960
StyD4I	CCNGG	5 cut(s)	604, 685, 1064, 1833, 2656
StyI	CCWWGG	5 cut(s)	408, 708, 907, 1198, 2460
TaaI	ACNGT	8 cut(s)	32, 85, 224, 1221, 1382, 2281, 2712, 3245
TaiI	ACGT	3 cut(s)	654, 2267, 3405
TaqII	GACCGA	1 cut(s)	1807
TatI	WGTACW	6 cut(s)	701, 1415, 1659, 1773, 2100, 2783
TauI	GCSGC	3 cut(s)	659, 2004, 3255
TfiI	GAWTC	4 cut(s)	230, 1795, 3326, 3360
TscAI	CASTG	7 cut(s)	754, 1171, 1224, 1277, 1720, 2715, 2747
TseFI	GTSAC	3 cut(s)	617, 1936, 2669
TseI	GCWGC	8 cut(s)	609, 1230, 1422, 2012, 2139, 2319, 2661, 2790
Tsp45I	GTSAC	3 cut(s)	617, 1936, 2669
TspGWI	ACGGA	4 cut(s)	223, 934, 1301, 2519
TspRI	CASTG	7 cut(s)	754, 1171, 1224, 1277, 1720, 2715, 2747
Van91I	CCANNNNNTGG	1 cut(s)	692
VneI	GTGCAC	2 cut(s)	1644, 2706
VpaK11BI	GGWCC	8 cut(s)	405, 412, 715, 1718, 1790, 2464, 3041, 3290
VspI	ATTAAT	2 cut(s)	1578, 2946
XagI	CCTNNNNNAGG	2 cut(s)	1947, 3198
XapI	RAATTY	5 cut(s)	487, 922, 1160, 1260, 2539
XceI	RCATGY	4 cut(s)	183, 1519, 2229, 3381
XhoI	CTCGAG	1 cut(s)	1546
XmiI	GTMKAC	1 cut(s)	1126
XmnI	GAANNNNTTC	2 cut(s)	1012, 1323
XspI	CTAG	4 cut(s)	1419, 2429, 2787, 3054
ZrmI	AGTACT	2 cut(s)	1417, 2785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.