A domain family that is part of the cupin metalloenzyme superfamily.
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 1108, 3053, 3145 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2957 |
| AccI | GTMKAC | 2 cut(s) | 931, 1207 |
| AccII | CGCG | 2 cut(s) | 229, 1938 |
| AccIII | TCCGGA | 1 cut(s) | 4405 |
| AclI | AACGTT | 1 cut(s) | 1893 |
| AclWI | GGATC | 3 cut(s) | 153, 823, 4413 |
| AcoI | YGGCCR | 5 cut(s) | 1187, 1592, 1939, 2902, 3554 |
| AcuI | CTGAAG | 6 cut(s) | 192, 867, 2004, 3243, 3593, 4020 |
| AcyI | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| AdeI | CACNNNGTG | 1 cut(s) | 3552 |
| AfaI | GTAC | 7 cut(s) | 1834, 2949, 3125, 3363, 3740, 4089, 4106 |
| AflIII | ACRYGT | 1 cut(s) | 2973 |
| AjiI | CACGTC | 1 cut(s) | 755 |
| AjnI | CCWGG | 5 cut(s) | 442, 994, 1651, 2827, 3987 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 765, 797 |
| Alw21I | GWGCWC | 2 cut(s) | 2966, 3252 |
| Alw26I | GTCTC | 6 cut(s) | 1840, 1960, 2140, 2227, 2795, 3067 |
| Alw44I | GTGCAC | 1 cut(s) | 2962 |
| AlwI | GGATC | 3 cut(s) | 153, 823, 4413 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2030, 2757 |
| Ama87I | CYCGRG | 1 cut(s) | 3165 |
| Aor13HI | TCCGGA | 1 cut(s) | 4405 |
| ApaLI | GTGCAC | 1 cut(s) | 2962 |
| ApeKI | GCWGC | 9 cut(s) | 681, 1856, 1859, 2003, 2011, 2757, 3374, 4463, 4545 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3959, 3991 |
| AseI | ATTAAT | 1 cut(s) | 3498 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 348, 2159, 4362 |
| AspLEI | GCGC | 5 cut(s) | 229, 231, 1111, 1938, 4456 |
| AspS9I | GGNCC | 6 cut(s) | 439, 942, 2685, 3532, 3728, 4336 |
| AsuC2I | CCSGG | 3 cut(s) | 628, 2152, 2239 |
| AvaI | CYCGRG | 1 cut(s) | 3165 |
| AvaII | GGWCC | 4 cut(s) | 439, 942, 2685, 3728 |
| AxyI | CCTNAGG | 2 cut(s) | 1431, 1947 |
| BaeGI | GKGCMC | 2 cut(s) | 2966, 3058 |
| BalI | TGGCCA | 2 cut(s) | 2904, 3556 |
| BanI | GGYRCC | 3 cut(s) | 1108, 3053, 3145 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 4015, 4018, 4047, 4050 |
| BauI | CACGAG | 1 cut(s) | 2680 |
| BbsI | GAAGAC | 1 cut(s) | 3972 |
| Bbv12I | GWGCWC | 2 cut(s) | 2966, 3252 |
| BbvCI | CCTCAGC | 2 cut(s) | 81, 1278 |
| BbvI | GCAGC | 8 cut(s) | 668, 1843, 1846, 2015, 2023, 2744, 3361, 4475 |
| BccI | CCATC | 7 cut(s) | 263, 1388, 1901, 2189, 3005, 3672, 4444 |
| BceAI | ACGGC | 3 cut(s) | 211, 1693, 3519 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 3617, 3651 |
| BciT130I | CCWGG | 5 cut(s) | 444, 996, 1653, 2829, 3989 |
| BciVI | GTATCC | 1 cut(s) | 2433 |
| BcnI | CCSGG | 3 cut(s) | 628, 2152, 2239 |
| BcoDI | GTCTC | 6 cut(s) | 1840, 1960, 2140, 2227, 2795, 3067 |
| BfaI | CTAG | 6 cut(s) | 1611, 1863, 2139, 2226, 2693, 4011 |
| BfmI | CTRYAG | 4 cut(s) | 2009, 2380, 2485, 4109 |
| BfoI | RGCGCY | 2 cut(s) | 1112, 4457 |
| BfuI | GTATCC | 1 cut(s) | 2433 |
| Bme1390I | CCNGG | 8 cut(s) | 444, 628, 996, 1653, 2152, 2239, 2829, 3989 |
| Bme18I | GGWCC | 4 cut(s) | 439, 942, 2685, 3728 |
| BmeT110I | CYCGRG | 1 cut(s) | 3165 |
| BmgBI | CACGTC | 1 cut(s) | 755 |
| BmgT120I | GGNCC | 6 cut(s) | 439, 942, 2685, 3532, 3728, 4336 |
| BmiI | GGNNCC | 9 cut(s) | 441, 622, 943, 1110, 1552, 3055, 3147, 3393, 3729 |
| BmrFI | CCNGG | 8 cut(s) | 444, 628, 996, 1653, 2152, 2239, 2829, 3989 |
| BmrI | ACTGGG | 1 cut(s) | 3558 |
| BmuI | ACTGGG | 1 cut(s) | 3558 |
| BpiI | GAAGAC | 1 cut(s) | 3972 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 25, 57 |
| BpmI | CTGGAG | 5 cut(s) | 900, 1523, 2850, 3222, 4010 |
| Bpu10I | CCTNAGC | 5 cut(s) | 42, 81, 1278, 3383, 4535 |
| BpuEI | CTTGAG | 3 cut(s) | 706, 2483, 3965 |
| BpuMI | CCSGG | 3 cut(s) | 628, 2152, 2239 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2703 |
| BsaHI | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| BsaI | GGTCTC | 2 cut(s) | 2140, 2227 |
| BsaJI | CCNNGG | 9 cut(s) | 259, 442, 2150, 2237, 3614, 3987, 4244, 4264, 4422 |
| BsaWI | WCCGGW | 1 cut(s) | 4405 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 873, 903, 2577, 2607, 3195, 3225, 4044, 4074 |
| Bse118I | RCCGGY | 1 cut(s) | 575 |
| Bse1I | ACTGG | 5 cut(s) | 2335, 3319, 3332, 3553, 3996 |
| Bse21I | CCTNAGG | 2 cut(s) | 1431, 1947 |
| Bse3DI | GCAATG | 2 cut(s) | 1168, 1687 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2703 |
| BseAI | TCCGGA | 1 cut(s) | 4405 |
| BseBI | CCWGG | 5 cut(s) | 444, 996, 1653, 2829, 3989 |
| BseDI | CCNNGG | 9 cut(s) | 259, 442, 2150, 2237, 3614, 3987, 4244, 4264, 4422 |
| BseJI | GATNNNNATC | 1 cut(s) | 2703 |
| BseMI | GCAATG | 2 cut(s) | 1168, 1687 |
| BseMII | CTCAG | 9 cut(s) | 56, 72, 197, 1269, 1551, 2262, 2616, 3062, 3374 |
| BseNI | ACTGG | 5 cut(s) | 2335, 3319, 3332, 3553, 3996 |
| BsePI | GCGCGC | 1 cut(s) | 227 |
| BseRI | GAGGAG | 6 cut(s) | 62, 2684, 3458, 3776, 4403, 4555 |
| BseSI | GKGCMC | 2 cut(s) | 2966, 3058 |
| BseX3I | CGGCCG | 1 cut(s) | 1939 |
| BseXI | GCAGC | 8 cut(s) | 668, 1843, 1846, 2015, 2023, 2744, 3361, 4475 |
| BseYI | CCCAGC | 2 cut(s) | 2522, 3387 |
| BsgI | GTGCAG | 2 cut(s) | 1393, 3779 |
| Bsh1236I | CGCG | 2 cut(s) | 229, 1938 |
| Bsh1285I | CGRYCG | 3 cut(s) | 935, 1826, 1942 |
| BshNI | GGYRCC | 3 cut(s) | 1108, 3053, 3145 |
| BsiEI | CGRYCG | 3 cut(s) | 935, 1826, 1942 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2966, 3252 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3165 |
| BsiSI | CCGG | 5 cut(s) | 576, 627, 2152, 2239, 4406 |
| BslFI | GGGAC | 4 cut(s) | 1033, 3311, 3741, 4079 |
| BsmAI | GTCTC | 6 cut(s) | 1840, 1960, 2140, 2227, 2795, 3067 |
| BsmBI | CGTCTC | 1 cut(s) | 3067 |
| BsmFI | GGGAC | 4 cut(s) | 1033, 3311, 3741, 4079 |
| BsmI | GAATGC | 4 cut(s) | 502, 1758, 2645, 4376 |
| Bso31I | GGTCTC | 2 cut(s) | 2140, 2227 |
| BsoBI | CYCGRG | 1 cut(s) | 3165 |
| Bsp1286I | GDGCHC | 3 cut(s) | 2966, 3058, 3252 |
| Bsp13I | TCCGGA | 1 cut(s) | 4405 |
| BspCNI | CTCAG | 9 cut(s) | 55, 73, 198, 1270, 1552, 2263, 2617, 3063, 3375 |
| BspEI | TCCGGA | 1 cut(s) | 4405 |
| BspFNI | CGCG | 2 cut(s) | 229, 1938 |
| BspLI | GGNNCC | 9 cut(s) | 441, 622, 943, 1110, 1552, 3055, 3147, 3393, 3729 |
| BspMAI | CTGCAG | 3 cut(s) | 2013, 2384, 2489 |
| BspPI | GGATC | 3 cut(s) | 153, 823, 4413 |
| BspQI | GCTCTTC | 1 cut(s) | 4463 |
| BspT107I | GGYRCC | 3 cut(s) | 1108, 3053, 3145 |
| BspTNI | GGTCTC | 2 cut(s) | 2140, 2227 |
| BsrDI | GCAATG | 2 cut(s) | 1168, 1687 |
| BsrFI | RCCGGY | 1 cut(s) | 575 |
| BsrI | ACTGG | 5 cut(s) | 2335, 3319, 3332, 3553, 3996 |
| BssAI | RCCGGY | 1 cut(s) | 575 |
| BssECI | CCNNGG | 9 cut(s) | 259, 442, 2150, 2237, 3614, 3987, 4244, 4264, 4422 |
| BssHII | GCGCGC | 1 cut(s) | 227 |
| BssNI | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| BssSI | CACGAG | 1 cut(s) | 2680 |
| BssT1I | CCWWGG | 4 cut(s) | 259, 3614, 4244, 4422 |
| Bst2BI | CACGAG | 1 cut(s) | 2680 |
| Bst2UI | CCWGG | 5 cut(s) | 444, 996, 1653, 2829, 3989 |
| Bst4CI | ACNGT | 4 cut(s) | 467, 1024, 2893, 3800 |
| Bst6I | CTCTTC | 6 cut(s) | 26, 98, 768, 843, 4221, 4463 |
| BstACI | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2493, 2563 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2501, 4268 |
| BstFNI | CGCG | 2 cut(s) | 229, 1938 |
| BstH2I | RGCGCY | 2 cut(s) | 1112, 4457 |
| BstHHI | GCGC | 5 cut(s) | 229, 231, 1111, 1938, 4456 |
| BstMAI | GTCTC | 6 cut(s) | 1840, 1960, 2140, 2227, 2795, 3067 |
| BstMCI | CGRYCG | 3 cut(s) | 935, 1826, 1942 |
| BstNI | CCWGG | 5 cut(s) | 444, 996, 1653, 2829, 3989 |
| BstNSI | RCATGY | 1 cut(s) | 2977 |
| BstSCI | CCNGG | 8 cut(s) | 442, 626, 994, 1651, 2150, 2237, 2827, 3987 |
| BstSFI | CTRYAG | 4 cut(s) | 2009, 2380, 2485, 4109 |
| BstSLI | GKGCMC | 2 cut(s) | 2966, 3058 |
| BstUI | CGCG | 2 cut(s) | 229, 1938 |
| BstV1I | GCAGC | 8 cut(s) | 668, 1843, 1846, 2015, 2023, 2744, 3361, 4475 |
| BstV2I | GAAGAC | 1 cut(s) | 3972 |
| BstX2I | RGATCY | 2 cut(s) | 145, 4418 |
| BstYI | RGATCY | 2 cut(s) | 145, 4418 |
| BstZI | CGGCCG | 1 cut(s) | 1939 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1431, 1947 |
| BsuI | GTATCC | 1 cut(s) | 2433 |
| BtgZI | GCGATG | 1 cut(s) | 3727 |
| BtrI | CACGTC | 1 cut(s) | 755 |
| BtsI | GCAGTG | 2 cut(s) | 1381, 2006 |
| BtsIMutI | CAGTG | 7 cut(s) | 1381, 2006, 2268, 2342, 3325, 3546, 3796 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2030, 2757 |
| CfoI | GCGC | 5 cut(s) | 229, 231, 1111, 1938, 4456 |
| Cfr10I | RCCGGY | 1 cut(s) | 575 |
| Cfr13I | GGNCC | 6 cut(s) | 439, 942, 2685, 3532, 3728, 4336 |
| CseI | GACGC | 4 cut(s) | 687, 1805, 2003, 2644 |
| Csp6I | GTAC | 7 cut(s) | 1833, 2948, 3124, 3362, 3739, 4088, 4105 |
| CviQI | GTAC | 7 cut(s) | 1833, 2948, 3124, 3362, 3739, 4088, 4105 |
| DinI | GGCGCC | 1 cut(s) | 1110 |
| DraI | TTTAAA | 1 cut(s) | 2376 |
| DraIII | CACNNNGTG | 1 cut(s) | 3552 |
| EaeI | YGGCCR | 5 cut(s) | 1187, 1592, 1939, 2902, 3554 |
| EagI | CGGCCG | 1 cut(s) | 1939 |
| Eam1104I | CTCTTC | 6 cut(s) | 26, 98, 768, 843, 4221, 4463 |
| EarI | CTCTTC | 6 cut(s) | 26, 98, 768, 843, 4221, 4463 |
| EciI | GGCGGA | 4 cut(s) | 381, 489, 633, 1290 |
| EclXI | CGGCCG | 1 cut(s) | 1939 |
| Eco130I | CCWWGG | 4 cut(s) | 259, 3614, 4244, 4422 |
| Eco147I | AGGCCT | 3 cut(s) | 594, 2137, 2224 |
| Eco31I | GGTCTC | 2 cut(s) | 2140, 2227 |
| Eco47I | GGWCC | 4 cut(s) | 439, 942, 2685, 3728 |
| Eco52I | CGGCCG | 1 cut(s) | 1939 |
| Eco57I | CTGAAG | 6 cut(s) | 192, 867, 2004, 3243, 3593, 4020 |
| Eco81I | CCTNAGG | 2 cut(s) | 1431, 1947 |
| Eco88I | CYCGRG | 1 cut(s) | 3165 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2501, 4268 |
| EcoO109I | RGGNCCY | 1 cut(s) | 439 |
| EcoRI | GAATTC | 4 cut(s) | 585, 3925, 4127, 4156 |
| EcoRII | CCWGG | 5 cut(s) | 442, 994, 1651, 2827, 3987 |
| EcoT14I | CCWWGG | 4 cut(s) | 259, 3614, 4244, 4422 |
| EcoT22I | ATGCAT | 2 cut(s) | 4328, 4376 |
| EgeI | GGCGCC | 1 cut(s) | 1110 |
| EheI | GGCGCC | 1 cut(s) | 1110 |
| ErhI | CCWWGG | 4 cut(s) | 259, 3614, 4244, 4422 |
| Esp3I | CGTCTC | 1 cut(s) | 3067 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1311, 1343, 2060, 2092, 3707, 3739 |
| FaqI | GGGAC | 4 cut(s) | 1033, 3311, 3741, 4079 |
| FauI | CCCGC | 3 cut(s) | 65, 194, 2643 |
| FblI | GTMKAC | 2 cut(s) | 931, 1207 |
| FspBI | CTAG | 6 cut(s) | 1611, 1863, 2139, 2226, 2693, 4011 |
| GlaI | GCGC | 5 cut(s) | 228, 230, 1110, 1937, 4455 |
| GsaI | CCCAGC | 2 cut(s) | 2526, 3391 |
| GsuI | CTGGAG | 5 cut(s) | 900, 1523, 2850, 3222, 4010 |
| HaeII | RGCGCY | 2 cut(s) | 1112, 4457 |
| HapII | CCGG | 5 cut(s) | 576, 627, 2152, 2239, 4406 |
| HgaI | GACGC | 4 cut(s) | 687, 1805, 2003, 2644 |
| HhaI | GCGC | 5 cut(s) | 229, 231, 1111, 1938, 4456 |
| Hin1I | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| Hin6I | GCGC | 5 cut(s) | 227, 229, 1109, 1936, 4454 |
| HinP1I | GCGC | 5 cut(s) | 227, 229, 1109, 1936, 4454 |
| HincII | GTYRAC | 1 cut(s) | 932 |
| HindII | GTYRAC | 1 cut(s) | 932 |
| HindIII | AAGCTT | 3 cut(s) | 1986, 3216, 3698 |
| HpaII | CCGG | 5 cut(s) | 576, 627, 2152, 2239, 4406 |
| Hpy166II | GTNNAC | 6 cut(s) | 92, 932, 1208, 1343, 2964, 3611 |
| Hpy8I | GTNNAC | 6 cut(s) | 92, 932, 1208, 1343, 2964, 3611 |
| Hpy99I | CGWCG | 2 cut(s) | 1053, 4080 |
| HpyCH4III | ACNGT | 4 cut(s) | 467, 1024, 2893, 3800 |
| HpyCH4IV | ACGT | 4 cut(s) | 754, 1893, 3076, 3737 |
| HpySE526I | ACGT | 4 cut(s) | 754, 1893, 3076, 3737 |
| Hsp92I | GRCGYC | 3 cut(s) | 1109, 1797, 2655 |
| HspAI | GCGC | 5 cut(s) | 227, 229, 1109, 1936, 4454 |
| KasI | GGCGCC | 1 cut(s) | 1108 |
| Kpn2I | TCCGGA | 1 cut(s) | 4405 |
| LguI | GCTCTTC | 1 cut(s) | 4463 |
| Lsp1109I | GCAGC | 8 cut(s) | 668, 1843, 1846, 2015, 2023, 2744, 3361, 4475 |
| MaeI | CTAG | 6 cut(s) | 1611, 1863, 2139, 2226, 2693, 4011 |
| MaeII | ACGT | 4 cut(s) | 754, 1893, 3076, 3737 |
| MfeI | CAATTG | 4 cut(s) | 2096, 2209, 2660, 2722 |
| MflI | RGATCY | 2 cut(s) | 145, 4418 |
| MhlI | GDGCHC | 3 cut(s) | 2966, 3058, 3252 |
| MlsI | TGGCCA | 2 cut(s) | 2904, 3556 |
| MluNI | TGGCCA | 2 cut(s) | 2904, 3556 |
| Mly113I | GGCGCC | 1 cut(s) | 1109 |
| MlyI | GAGTC | 4 cut(s) | 618, 736, 4001, 4180 |
| MmeI | TCCRAC | 5 cut(s) | 409, 1369, 2632, 4031, 4402 |
| Mox20I | TGGCCA | 2 cut(s) | 2904, 3556 |
| Mph1103I | ATGCAT | 2 cut(s) | 4328, 4376 |
| MroI | TCCGGA | 1 cut(s) | 4405 |
| MroXI | GAANNNNTTC | 3 cut(s) | 348, 2159, 4362 |
| MscI | TGGCCA | 2 cut(s) | 2904, 3556 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1680, 3255, 4331 |
| Msp20I | TGGCCA | 2 cut(s) | 2904, 3556 |
| MspA1I | CMGCKG | 1 cut(s) | 3865 |
| MspI | CCGG | 5 cut(s) | 576, 627, 2152, 2239, 4406 |
| MspR9I | CCNGG | 8 cut(s) | 444, 628, 996, 1653, 2152, 2239, 2829, 3989 |
| MssI | GTTTAAAC | 1 cut(s) | 2376 |
| MunI | CAATTG | 4 cut(s) | 2096, 2209, 2660, 2722 |
| Mva1269I | GAATGC | 4 cut(s) | 502, 1758, 2645, 4376 |
| MvaI | CCWGG | 5 cut(s) | 444, 996, 1653, 2829, 3989 |
| MvnI | CGCG | 2 cut(s) | 229, 1938 |
| NarI | GGCGCC | 1 cut(s) | 1109 |
| NciI | CCSGG | 3 cut(s) | 628, 2152, 2239 |
| NlaIV | GGNNCC | 9 cut(s) | 441, 622, 943, 1110, 1552, 3055, 3147, 3393, 3729 |
| NmeAIII | GCCGAG | 1 cut(s) | 4245 |
| NmuCI | GTSAC | 7 cut(s) | 1121, 1699, 2717, 2893, 2969, 3653, 3800 |
| NsiI | ATGCAT | 2 cut(s) | 4328, 4376 |
| NspI | RCATGY | 1 cut(s) | 2977 |
| PaeR7I | CTCGAG | 1 cut(s) | 3165 |
| PauI | GCGCGC | 1 cut(s) | 227 |
| PceI | AGGCCT | 3 cut(s) | 594, 2137, 2224 |
| PciI | ACATGT | 1 cut(s) | 2973 |
| PciSI | GCTCTTC | 1 cut(s) | 4463 |
| PctI | GAATGC | 4 cut(s) | 502, 1758, 2645, 4376 |
| PdmI | GAANNNNTTC | 3 cut(s) | 348, 2159, 4362 |
| PfeI | GAWTC | 6 cut(s) | 2846, 2908, 3019, 3779, 3850, 4511 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2957 |
| Ple19I | CGATCG | 1 cut(s) | 1826 |
| PleI | GAGTC | 4 cut(s) | 617, 735, 4000, 4180 |
| PluTI | GGCGCC | 1 cut(s) | 1112 |
| PmeI | GTTTAAAC | 1 cut(s) | 2376 |
| PpsI | GAGTC | 4 cut(s) | 617, 735, 4000, 4180 |
| PpuMI | RGGWCCY | 1 cut(s) | 439 |
| PscI | ACATGT | 1 cut(s) | 2973 |
| PshBI | ATTAAT | 1 cut(s) | 3498 |
| Psp1406I | AACGTT | 1 cut(s) | 1893 |
| Psp5II | RGGWCCY | 1 cut(s) | 439 |
| Psp6I | CCWGG | 5 cut(s) | 442, 994, 1651, 2827, 3987 |
| PspFI | CCCAGC | 2 cut(s) | 2522, 3387 |
| PspGI | CCWGG | 5 cut(s) | 442, 994, 1651, 2827, 3987 |
| PspN4I | GGNNCC | 9 cut(s) | 441, 622, 943, 1110, 1552, 3055, 3147, 3393, 3729 |
| PspPI | GGNCC | 6 cut(s) | 439, 942, 2685, 3532, 3728, 4336 |
| PspPPI | RGGWCCY | 1 cut(s) | 439 |
| PstI | CTGCAG | 3 cut(s) | 2013, 2384, 2489 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2030, 2757 |
| PsuI | RGATCY | 2 cut(s) | 145, 4418 |
| PteI | GCGCGC | 1 cut(s) | 227 |
| PvuI | CGATCG | 1 cut(s) | 1826 |
| RsaI | GTAC | 7 cut(s) | 1834, 2949, 3125, 3363, 3740, 4089, 4106 |
| RsaNI | GTAC | 7 cut(s) | 1833, 2948, 3124, 3362, 3739, 4088, 4105 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1680, 3255, 4331 |
| SalI | GTCGAC | 1 cut(s) | 930 |
| SapI | GCTCTTC | 1 cut(s) | 4463 |
| Sau96I | GGNCC | 6 cut(s) | 439, 942, 2685, 3532, 3728, 4336 |
| SchI | GAGTC | 4 cut(s) | 618, 736, 4001, 4180 |
| ScrFI | CCNGG | 8 cut(s) | 444, 628, 996, 1653, 2152, 2239, 2829, 3989 |
| SduI | GDGCHC | 3 cut(s) | 2966, 3058, 3252 |
| SfcI | CTRYAG | 4 cut(s) | 2009, 2380, 2485, 4109 |
| SfoI | GGCGCC | 1 cut(s) | 1110 |
| Sfr274I | CTCGAG | 1 cut(s) | 3165 |
| SinI | GGWCC | 4 cut(s) | 439, 942, 2685, 3728 |
| SlaI | CTCGAG | 1 cut(s) | 3165 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1680, 3255, 4331 |
| SmlI | CTYRAG | 4 cut(s) | 685, 2498, 3165, 3944 |
| SmoI | CTYRAG | 4 cut(s) | 685, 2498, 3165, 3944 |
| SseBI | AGGCCT | 3 cut(s) | 594, 2137, 2224 |
| SspDI | GGCGCC | 1 cut(s) | 1108 |
| SspI | AATATT | 4 cut(s) | 1269, 1624, 3502, 4363 |
| SspMI | CTAG | 6 cut(s) | 1611, 1863, 2139, 2226, 2693, 4011 |
| StuI | AGGCCT | 3 cut(s) | 594, 2137, 2224 |
| StyD4I | CCNGG | 8 cut(s) | 442, 626, 994, 1651, 2150, 2237, 2827, 3987 |
| StyI | CCWWGG | 4 cut(s) | 259, 3614, 4244, 4422 |
| TaaI | ACNGT | 4 cut(s) | 467, 1024, 2893, 3800 |
| TaiI | ACGT | 4 cut(s) | 757, 1896, 3079, 3740 |
| TaqII | GACCGA | 1 cut(s) | 949 |
| TatI | WGTACW | 1 cut(s) | 3361 |
| TauI | GCSGC | 7 cut(s) | 201, 255, 258, 474, 626, 1941, 3239 |
| TfiI | GAWTC | 6 cut(s) | 2846, 2908, 3019, 3779, 3850, 4511 |
| TscAI | CASTG | 7 cut(s) | 1381, 2013, 2275, 2342, 3332, 3553, 3803 |
| TseFI | GTSAC | 7 cut(s) | 1121, 1699, 2717, 2893, 2969, 3653, 3800 |
| TseI | GCWGC | 9 cut(s) | 681, 1856, 1859, 2003, 2011, 2757, 3374, 4463, 4545 |
| Tsp45I | GTSAC | 7 cut(s) | 1121, 1699, 2717, 2893, 2969, 3653, 3800 |
| TspGWI | ACGGA | 5 cut(s) | 1687, 2250, 3854, 4064, 4536 |
| TspRI | CASTG | 7 cut(s) | 1381, 2013, 2275, 2342, 3332, 3553, 3803 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2957 |
| VneI | GTGCAC | 1 cut(s) | 2962 |
| VpaK11BI | GGWCC | 4 cut(s) | 439, 942, 2685, 3728 |
| VspI | ATTAAT | 1 cut(s) | 3498 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2501, 4268 |
| XceI | RCATGY | 1 cut(s) | 2977 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2831, 3402 |
| XhoI | CTCGAG | 1 cut(s) | 3165 |
| XmiI | GTMKAC | 2 cut(s) | 931, 1207 |
| XmnI | GAANNNNTTC | 3 cut(s) | 348, 2159, 4362 |
| XspI | CTAG | 6 cut(s) | 1611, 1863, 2139, 2226, 2693, 4011 |
| Zsp2I | ATGCAT | 2 cut(s) | 4328, 4376 |