A domain family that is part of the cupin metalloenzyme superfamily.
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 912 |
| AccB1I | GGYRCC | 1 cut(s) | 785 |
| AccI | GTMKAC | 2 cut(s) | 775, 904 |
| AciI | CCGC | 4 cut(s) | 89, 153, 165, 447 |
| AclWI | GGATC | 2 cut(s) | 129, 667 |
| AcsI | RAATTY | 3 cut(s) | 105, 348, 371 |
| AcuI | CTGAAG | 2 cut(s) | 711, 938 |
| AfaI | GTAC | 1 cut(s) | 937 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 842 |
| AgsI | TTSAA | 4 cut(s) | 329, 344, 353, 1043 |
| AjnI | CCWGG | 1 cut(s) | 240 |
| AluBI | AGCT | 5 cut(s) | 56, 202, 270, 689, 769 |
| AluI | AGCT | 5 cut(s) | 56, 202, 270, 689, 769 |
| Alw21I | GWGCWC | 1 cut(s) | 625 |
| Alw26I | GTCTC | 1 cut(s) | 960 |
| AlwI | GGATC | 2 cut(s) | 129, 667 |
| AoxI | GGCC | 5 cut(s) | 439, 463, 501, 650, 884 |
| ApeKI | GCWGC | 1 cut(s) | 53 |
| ApoI | RAATTY | 3 cut(s) | 105, 348, 371 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 348, 1038 |
| AspS9I | GGNCC | 1 cut(s) | 884 |
| BanI | GGYRCC | 1 cut(s) | 785 |
| Bbv12I | GWGCWC | 1 cut(s) | 625 |
| BbvCI | CCTCAGC | 1 cut(s) | 984 |
| BbvI | GCAGC | 1 cut(s) | 65 |
| BccI | CCATC | 5 cut(s) | 248, 398, 812, 833, 947 |
| BciT130I | CCWGG | 1 cut(s) | 242 |
| BcoDI | GTCTC | 1 cut(s) | 960 |
| BfaI | CTAG | 1 cut(s) | 770 |
| BisI | GCNGC | 1 cut(s) | 54 |
| BlsI | GCNGC | 1 cut(s) | 55 |
| Bme1390I | CCNGG | 1 cut(s) | 242 |
| BmgT120I | GGNCC | 1 cut(s) | 884 |
| BmiI | GGNNCC | 1 cut(s) | 787 |
| BmrFI | CCNGG | 1 cut(s) | 242 |
| BmsI | GCATC | 1 cut(s) | 563 |
| Bpu10I | CCTNAGC | 1 cut(s) | 984 |
| BpuEI | CTTGAG | 1 cut(s) | 609 |
| BsaJI | CCNNGG | 2 cut(s) | 241, 442 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 842 |
| Bse1I | ACTGG | 2 cut(s) | 727, 1024 |
| BseBI | CCWGG | 1 cut(s) | 242 |
| BseDI | CCNNGG | 2 cut(s) | 241, 442 |
| BseGI | GGATG | 1 cut(s) | 409 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 842 |
| BseMII | CTCAG | 1 cut(s) | 975 |
| BseNI | ACTGG | 2 cut(s) | 727, 1024 |
| BseRI | GAGGAG | 1 cut(s) | 155 |
| BseXI | GCAGC | 1 cut(s) | 65 |
| BseYI | CCCAGC | 1 cut(s) | 299 |
| BshFI | GGCC | 5 cut(s) | 441, 465, 503, 652, 886 |
| BshNI | GGYRCC | 1 cut(s) | 785 |
| BsiHKAI | GWGCWC | 1 cut(s) | 625 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 842 |
| BsmAI | GTCTC | 1 cut(s) | 960 |
| BsmI | GAATGC | 2 cut(s) | 417, 462 |
| BsnI | GGCC | 5 cut(s) | 441, 465, 503, 652, 886 |
| Bsp1286I | GDGCHC | 1 cut(s) | 625 |
| Bsp143I | GATC | 2 cut(s) | 121, 659 |
| BspACI | CCGC | 4 cut(s) | 89, 153, 165, 447 |
| BspANI | GGCC | 5 cut(s) | 441, 465, 503, 652, 886 |
| BspCNI | CTCAG | 1 cut(s) | 976 |
| BspLI | GGNNCC | 1 cut(s) | 787 |
| BspPI | GGATC | 2 cut(s) | 129, 667 |
| BspT107I | GGYRCC | 1 cut(s) | 785 |
| BsrI | ACTGG | 2 cut(s) | 727, 1024 |
| BssECI | CCNNGG | 2 cut(s) | 241, 442 |
| BssMI | GATC | 2 cut(s) | 121, 659 |
| BssT1I | CCWWGG | 1 cut(s) | 442 |
| Bst2UI | CCWGG | 1 cut(s) | 242 |
| Bst4CI | ACNGT | 1 cut(s) | 940 |
| Bst6I | CTCTTC | 3 cut(s) | 687, 738, 755 |
| BstC8I | GCNNGC | 1 cut(s) | 1013 |
| BstDEI | CTNAG | 1 cut(s) | 984 |
| BstF5I | GGATG | 1 cut(s) | 409 |
| BstKTI | GATC | 2 cut(s) | 124, 662 |
| BstMAI | GTCTC | 1 cut(s) | 960 |
| BstMBI | GATC | 2 cut(s) | 121, 659 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 649 |
| BstNI | CCWGG | 1 cut(s) | 242 |
| BstSCI | CCNGG | 1 cut(s) | 240 |
| BstV1I | GCAGC | 1 cut(s) | 65 |
| BstX2I | RGATCY | 1 cut(s) | 121 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1019 |
| BstYI | RGATCY | 1 cut(s) | 121 |
| BsuRI | GGCC | 5 cut(s) | 441, 465, 503, 652, 886 |
| BtsCI | GGATG | 1 cut(s) | 409 |
| BtsI | GCAGTG | 1 cut(s) | 867 |
| BtsIMutI | CAGTG | 1 cut(s) | 867 |
| Cac8I | GCNNGC | 1 cut(s) | 1013 |
| Cfr13I | GGNCC | 1 cut(s) | 884 |
| Csp6I | GTAC | 1 cut(s) | 936 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 718, 753 |
| CviQI | GTAC | 1 cut(s) | 936 |
| DdeI | CTNAG | 1 cut(s) | 984 |
| DpnI | GATC | 2 cut(s) | 123, 661 |
| DpnII | GATC | 2 cut(s) | 121, 659 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 912 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 912 |
| Eam1104I | CTCTTC | 3 cut(s) | 687, 738, 755 |
| EarI | CTCTTC | 3 cut(s) | 687, 738, 755 |
| Eco130I | CCWWGG | 1 cut(s) | 442 |
| Eco147I | AGGCCT | 3 cut(s) | 441, 465, 503 |
| Eco57I | CTGAAG | 2 cut(s) | 711, 938 |
| EcoRII | CCWGG | 1 cut(s) | 240 |
| EcoT14I | CCWWGG | 1 cut(s) | 442 |
| EcoT22I | ATGCAT | 1 cut(s) | 556 |
| ErhI | CCWWGG | 1 cut(s) | 442 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1023 |
| FauI | CCCGC | 1 cut(s) | 82 |
| FblI | GTMKAC | 2 cut(s) | 775, 904 |
| Fnu4HI | GCNGC | 1 cut(s) | 54 |
| FokI | GGATG | 1 cut(s) | 416 |
| Fsp4HI | GCNGC | 1 cut(s) | 54 |
| FspBI | CTAG | 1 cut(s) | 770 |
| GluI | GCNGC | 1 cut(s) | 54 |
| GsaI | CCCAGC | 1 cut(s) | 303 |
| HaeIII | GGCC | 5 cut(s) | 441, 465, 503, 652, 886 |
| HincII | GTYRAC | 2 cut(s) | 776, 905 |
| HindII | GTYRAC | 2 cut(s) | 776, 905 |
| HindIII | AAGCTT | 1 cut(s) | 200 |
| Hpy166II | GTNNAC | 2 cut(s) | 776, 905 |
| Hpy188I | TCNGA | 3 cut(s) | 918, 1008, 1027 |
| Hpy188III | TCNNGA | 5 cut(s) | 341, 401, 558, 587, 963 |
| Hpy8I | GTNNAC | 2 cut(s) | 776, 905 |
| HpyAV | CCTTC | 4 cut(s) | 7, 431, 446, 796 |
| HpyCH4III | ACNGT | 1 cut(s) | 940 |
| HpyCH4V | TGCA | 3 cut(s) | 460, 554, 812 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 649 |
| HpyF3I | CTNAG | 1 cut(s) | 984 |
| Kzo9I | GATC | 2 cut(s) | 121, 659 |
| LmnI | GCTCC | 1 cut(s) | 686 |
| Lsp1109I | GCAGC | 1 cut(s) | 65 |
| LweI | GCATC | 1 cut(s) | 563 |
| MaeI | CTAG | 1 cut(s) | 770 |
| MaeIII | GTNAC | 3 cut(s) | 98, 236, 751 |
| MalI | GATC | 2 cut(s) | 123, 661 |
| MboI | GATC | 2 cut(s) | 121, 659 |
| MflI | RGATCY | 1 cut(s) | 121 |
| MhlI | GDGCHC | 1 cut(s) | 625 |
| MluCI | AATT | 7 cut(s) | 105, 192, 348, 371, 699, 717, 990 |
| Mph1103I | ATGCAT | 1 cut(s) | 556 |
| MroXI | GAANNNNTTC | 2 cut(s) | 348, 1038 |
| MseI | TTAA | 1 cut(s) | 20 |
| MspA1I | CMGCKG | 1 cut(s) | 56 |
| MspR9I | CCNGG | 1 cut(s) | 242 |
| Mva1269I | GAATGC | 2 cut(s) | 417, 462 |
| MvaI | CCWGG | 1 cut(s) | 242 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 649 |
| NdeII | GATC | 2 cut(s) | 121, 659 |
| NlaIV | GGNNCC | 1 cut(s) | 787 |
| NmuCI | GTSAC | 2 cut(s) | 98, 236 |
| NsiI | ATGCAT | 1 cut(s) | 556 |
| PceI | AGGCCT | 3 cut(s) | 441, 465, 503 |
| PctI | GAATGC | 2 cut(s) | 417, 462 |
| PdmI | GAANNNNTTC | 2 cut(s) | 348, 1038 |
| PkrI | GCNGC | 1 cut(s) | 55 |
| Psp6I | CCWGG | 1 cut(s) | 240 |
| PspFI | CCCAGC | 1 cut(s) | 299 |
| PspGI | CCWGG | 1 cut(s) | 240 |
| PspN4I | GGNNCC | 1 cut(s) | 787 |
| PspPI | GGNCC | 1 cut(s) | 884 |
| PsuI | RGATCY | 1 cut(s) | 121 |
| PvuII | CAGCTG | 1 cut(s) | 56 |
| RsaI | GTAC | 1 cut(s) | 937 |
| RsaNI | GTAC | 1 cut(s) | 936 |
| SalI | GTCGAC | 2 cut(s) | 774, 903 |
| SaqAI | TTAA | 1 cut(s) | 20 |
| SatI | GCNGC | 1 cut(s) | 54 |
| Sau3AI | GATC | 2 cut(s) | 121, 659 |
| Sau96I | GGNCC | 1 cut(s) | 884 |
| ScrFI | CCNGG | 1 cut(s) | 242 |
| SduI | GDGCHC | 1 cut(s) | 625 |
| SfaNI | GCATC | 1 cut(s) | 563 |
| SmlI | CTYRAG | 1 cut(s) | 624 |
| SmoI | CTYRAG | 1 cut(s) | 624 |
| Sse9I | AATT | 7 cut(s) | 105, 192, 348, 371, 699, 717, 990 |
| SseBI | AGGCCT | 3 cut(s) | 441, 465, 503 |
| SsiI | CCGC | 4 cut(s) | 89, 153, 165, 447 |
| SspI | AATATT | 1 cut(s) | 682 |
| SspMI | CTAG | 1 cut(s) | 770 |
| StuI | AGGCCT | 3 cut(s) | 441, 465, 503 |
| StyD4I | CCNGG | 1 cut(s) | 240 |
| StyI | CCWWGG | 1 cut(s) | 442 |
| TaaI | ACNGT | 1 cut(s) | 940 |
| TaqI | TCGA | 3 cut(s) | 632, 775, 904 |
| TasI | AATT | 7 cut(s) | 105, 192, 348, 371, 699, 717, 990 |
| Tru1I | TTAA | 1 cut(s) | 20 |
| Tru9I | TTAA | 1 cut(s) | 20 |
| TscAI | CASTG | 1 cut(s) | 867 |
| TseFI | GTSAC | 2 cut(s) | 98, 236 |
| TseI | GCWGC | 1 cut(s) | 53 |
| Tsp45I | GTSAC | 2 cut(s) | 98, 236 |
| TspDTI | ATGAA | 2 cut(s) | 204, 450 |
| TspRI | CASTG | 1 cut(s) | 867 |
| XapI | RAATTY | 3 cut(s) | 105, 348, 371 |
| XmiI | GTMKAC | 2 cut(s) | 775, 904 |
| XmnI | GAANNNNTTC | 2 cut(s) | 348, 1038 |
| XspI | CTAG | 1 cut(s) | 770 |
| Zsp2I | ATGCAT | 1 cut(s) | 556 |