pycom05g28100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001486
Physical Location & Seq
Reverse (-)
28928363 .. 28930292
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGGCTTGGTATTTTCGGGCAAGTTTGACCATGAACGTCCTCCTACTCGTCGGAACAGTTGTGATCATCCCACATGCGCTTGGTTGCTACACTTCAATCATTGGCTTTGGGGATTCAACTGCTGATACAGGCAACTCATACCTCAATGCCCTTTACCACCATGACGAGCCTTTCCACTTCTTCTTCCCTCCATATGGGGAGGACTTCTTTCATCACCCCACCGGCCGCTGCTGCGACGGTCGTTTAATCATAGACTTCATTGCTGAATCTCTGGGACTTCCTCTTGTGCGACCTTATTTTGAAATCCTGAACGTCAATGAAACAGTGCGCAACTTTGAAGAGGGTGTGAATTTTGCAGTAGTAGGAGCCACTGCACTTGACACTGACTTTCTTGAACAAATGGGACATTATAATGAATTTACAAACTTCTCTCTAAGAATTCAAGTGGATTGGTTCAAAGGAATGTTATCACCTTTATGCAATACAGCTTCGGACTGCAGGGATCTCCTCTCAAGTTCTCTAGTTCTTATGGGACCGATTGGAGGCAACGATTACACCCATGCGTTGTTTGCAGGAGACAGTTTTCAAATTGTTCGAACATTTGTTCCAGTAGTGATGGAAGCAATGGCTTCAGCTATCAATGAGTTGATTGAGCATGGGGCATCAACACTTTTGGTACCAGGCAACTTTCCAATTGGTTGCCTCCCTACTTATCTTACATTCTATGAGGGTTCAGATGAGAGTGAATACGATCCTTCAACTGGTTGTCTTACTTGGTTAAACAAGTTTTCTGAGTATCACAATGAGCAGCTTCAGATAAAGCTAAGTCAGATTCGAAGCCTTCATCCTCATGCTAACATCATTTATGGAGATGTTTACAGTGCTACGTTACAACTGTATCAGTCTCCAAAACAATTTGGATTTACTTGGGAAGCAACCAAAGCATGCTGTGGAGGAGGGGGGACATACAATTACAACTCATCAGCACAATGTGGTGTTCCACCGGCAAGTGCTTGTCCAAACCCTGAAGAGGCTATCGACTGGGACGGTCTGCACTGTACTGAAGGAGCCAACCGGTGGATAGCCAAGGCTTTATTATATGGAAATTATACCATTCCCCGCCTTAGTAACTCCTGTGTGTCACAACTGTGA

Protein Analysis

384

Amino Acids

42.42

Weight (kDa)

4.77

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 365 9.2e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
Acc16I TGCGCA 1 cut(s) 329
Acc65I GGTACC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 676
AccB7I CCANNNNNTGG 1 cut(s) 1077
AciI CCGC 2 cut(s) 226, 1120
AclWI GGATC 2 cut(s) 510, 746
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 3 cut(s) 349, 416, 438
AcuI CTGAAG 4 cut(s) 615, 797, 1047, 1083
AdeI CACNNNGTG 1 cut(s) 992
AfaI GTAC 2 cut(s) 678, 1060
AfiI CCNNNNNNNGG 5 cut(s) 194, 542, 761, 1030, 1077
AgeI ACCGGT 1 cut(s) 1074
AgsI TTSAA 9 cut(s) 96, 117, 302, 338, 395, 443, 457, 587, 759
AjnI CCWGG 1 cut(s) 679
AleI CACNNNNGTG 1 cut(s) 1147
AloI GAACNNNNNNTCC 2 cut(s) 589, 621
AluBI AGCT 4 cut(s) 488, 635, 811, 823
AluI AGCT 4 cut(s) 488, 635, 811, 823
Alw26I GTCTC 2 cut(s) 570, 909
AlwI GGATC 2 cut(s) 510, 746
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 228, 231, 808
ApoI RAATTY 3 cut(s) 349, 416, 438
AsiGI ACCGGT 1 cut(s) 1074
Asp718I GGTACC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 79, 330
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 206, 462
AsuII TTCGAA 2 cut(s) 595, 835
AvaII GGWCC 1 cut(s) 533
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BanI GGYRCC 1 cut(s) 676
BbvI GCAGC 3 cut(s) 215, 218, 820
BccI CCATC 1 cut(s) 610
BciT130I CCWGG 1 cut(s) 681
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 570, 909
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 4 cut(s) 226, 229, 232, 809
BlsI GCNGC 4 cut(s) 227, 230, 233, 810
Bme1390I CCNGG 1 cut(s) 681
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 4 cut(s) 367, 534, 678, 1069
BmrFI CCNGG 1 cut(s) 681
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 1 cut(s) 671
BmuI ACTGGG 1 cut(s) 1051
Bpu14I TTCGAA 2 cut(s) 595, 835
BpuEI CTTGAG 1 cut(s) 496
BsaJI CCNNGG 1 cut(s) 1086
BsaWI WCCGGW 1 cut(s) 1074
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 542, 761, 1030, 1077
Bse118I RCCGGY 3 cut(s) 221, 1003, 1074
Bse1I ACTGG 3 cut(s) 608, 766, 1046
Bse3DI GCAATG 2 cut(s) 258, 630
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 1 cut(s) 1086
BseGI GGATG 2 cut(s) 66, 844
BseLI CCNNNNNNNGG 5 cut(s) 194, 542, 761, 1030, 1077
BseMI GCAATG 2 cut(s) 258, 630
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 3 cut(s) 608, 766, 1046
BseRI GAGGAG 2 cut(s) 497, 969
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 3 cut(s) 215, 218, 820
BsgI GTGCAG 2 cut(s) 357, 1037
Bsh1285I CGRYCG 2 cut(s) 226, 241
BshFI GGCC 1 cut(s) 225
BshNI GGYRCC 1 cut(s) 676
BshTI ACCGGT 1 cut(s) 1074
BsiEI CGRYCG 2 cut(s) 226, 241
BsiSI CCGG 3 cut(s) 222, 1004, 1075
BslFI GGGAC 5 cut(s) 288, 417, 546, 976, 1058
BslI CCNNNNNNNGG 5 cut(s) 194, 542, 761, 1030, 1077
BsmAI GTCTC 2 cut(s) 570, 909
BsmFI GGGAC 5 cut(s) 288, 417, 546, 976, 1058
BsnI GGCC 1 cut(s) 225
Bsp119I TTCGAA 2 cut(s) 595, 835
Bsp143I GATC 3 cut(s) 63, 502, 751
BspACI CCGC 2 cut(s) 226, 1120
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 784
BspLI GGNNCC 4 cut(s) 367, 534, 678, 1069
BspMAI CTGCAG 1 cut(s) 500
BspPI GGATC 2 cut(s) 510, 746
BspT104I TTCGAA 2 cut(s) 595, 835
BspT107I GGYRCC 1 cut(s) 676
BsrDI GCAATG 2 cut(s) 258, 630
BsrFI RCCGGY 3 cut(s) 221, 1003, 1074
BsrI ACTGG 3 cut(s) 608, 766, 1046
BssAI RCCGGY 3 cut(s) 221, 1003, 1074
BssECI CCNNGG 1 cut(s) 1086
BssMI GATC 3 cut(s) 63, 502, 751
BssT1I CCWWGG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 9 cut(s) 58, 239, 325, 581, 881, 897, 1049, 1058, 1149
Bst6I CTCTTC 2 cut(s) 333, 1023
BstBI TTCGAA 2 cut(s) 595, 835
BstC8I GCNNGC 1 cut(s) 946
BstDEI CTNAG 4 cut(s) 434, 792, 824, 1124
BstF5I GGATG 2 cut(s) 66, 844
BstHHI GCGC 2 cut(s) 79, 330
BstKTI GATC 3 cut(s) 66, 505, 754
BstMAI GTCTC 2 cut(s) 570, 909
BstMBI GATC 3 cut(s) 63, 502, 751
BstMCI CGRYCG 2 cut(s) 226, 241
BstMWI GCNNNNNNNGC 3 cut(s) 231, 569, 941
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 2 cut(s) 77, 948
BstSCI CCNGG 1 cut(s) 679
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 215, 218, 820
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 66, 844
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 5 cut(s) 330, 369, 381, 886, 1054
Cac8I GCNNGC 1 cut(s) 946
CfoI GCGC 2 cut(s) 79, 330
Cfr10I RCCGGY 3 cut(s) 221, 1003, 1074
Cfr13I GGNCC 1 cut(s) 533
Csp6I GTAC 2 cut(s) 677, 1059
CspAI ACCGGT 1 cut(s) 1074
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 7 cut(s) 31, 74, 161, 560, 656, 851, 945
CviQI GTAC 2 cut(s) 677, 1059
DdeI CTNAG 4 cut(s) 434, 792, 824, 1124
DpnI GATC 3 cut(s) 65, 504, 753
DpnII GATC 3 cut(s) 63, 502, 751
DraIII CACNNNGTG 1 cut(s) 992
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 333, 1023
EarI CTCTTC 2 cut(s) 333, 1023
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 533
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 4 cut(s) 615, 797, 1047, 1083
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 1086
ErhI CCWWGG 1 cut(s) 1086
FaeI CATG 7 cut(s) 34, 77, 164, 563, 659, 854, 948
FaqI GGGAC 5 cut(s) 288, 417, 546, 976, 1058
FatI CATG 7 cut(s) 30, 73, 160, 559, 655, 850, 944
FauI CCCGC 1 cut(s) 1127
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 226, 229, 232, 809
FokI GGATG 2 cut(s) 53, 831
Fsp4HI GCNGC 4 cut(s) 226, 229, 232, 809
FspBI CTAG 1 cut(s) 521
FspI TGCGCA 1 cut(s) 329
GlaI GCGC 2 cut(s) 78, 329
GluI GCNGC 4 cut(s) 226, 229, 232, 809
HaeIII GGCC 1 cut(s) 225
HapII CCGG 3 cut(s) 222, 1004, 1075
HhaI GCGC 2 cut(s) 79, 330
Hin1II CATG 7 cut(s) 34, 77, 164, 563, 659, 854, 948
Hin6I GCGC 2 cut(s) 77, 328
HinP1I GCGC 2 cut(s) 77, 328
HinfI GANTC 3 cut(s) 113, 266, 832
HpaII CCGG 3 cut(s) 222, 1004, 1075
HphI GGTGA 2 cut(s) 206, 462
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 6 cut(s) 53, 493, 736, 793, 816, 831
Hpy188III TCNNGA 2 cut(s) 307, 392
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 2 cut(s) 53, 239
HpyAV CCTTC 3 cut(s) 765, 851, 1058
HpyCH4III ACNGT 9 cut(s) 58, 239, 325, 581, 881, 897, 1049, 1058, 1149
HpyCH4IV ACGT 3 cut(s) 36, 312, 887
HpyCH4V TGCA 6 cut(s) 356, 374, 480, 498, 572, 1054
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 569, 941
HpyF3I CTNAG 4 cut(s) 434, 792, 824, 1124
HpySE526I ACGT 3 cut(s) 36, 312, 887
Hsp92II CATG 7 cut(s) 34, 77, 164, 563, 659, 854, 948
HspAI GCGC 2 cut(s) 77, 328
KpnI GGTACC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 3 cut(s) 63, 502, 751
LmnI GCTCC 2 cut(s) 365, 1067
Lsp1109I GCAGC 3 cut(s) 215, 218, 820
LweI GCATC 1 cut(s) 671
MaeI CTAG 1 cut(s) 521
MaeII ACGT 3 cut(s) 36, 312, 887
MaeIII GTNAC 3 cut(s) 888, 1127, 1140
MalI GATC 3 cut(s) 65, 504, 753
MboI GATC 3 cut(s) 63, 502, 751
MboII GAAGA 4 cut(s) 172, 175, 350, 1040
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 1 cut(s) 502
MluCI AATT 8 cut(s) 349, 416, 438, 588, 693, 914, 970, 1105
MmeI TCCRAC 1 cut(s) 31
MseI TTAA 2 cut(s) 245, 779
MslI CAYNNNNRTG 4 cut(s) 411, 475, 849, 1147
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 3 cut(s) 222, 1004, 1075
MspR9I CCNGG 1 cut(s) 681
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 681
MwoI GCNNNNNNNGC 3 cut(s) 231, 569, 941
NdeI CATATG 1 cut(s) 193
NdeII GATC 3 cut(s) 63, 502, 751
NlaIII CATG 7 cut(s) 34, 77, 164, 563, 659, 854, 948
NlaIV GGNNCC 4 cut(s) 367, 534, 678, 1069
NmuCI GTSAC 1 cut(s) 1140
NsbI TGCGCA 1 cut(s) 329
NspI RCATGY 2 cut(s) 77, 948
NspV TTCGAA 2 cut(s) 595, 835
OliI CACNNNNGTG 1 cut(s) 1147
PaeI GCATGC 1 cut(s) 948
PfeI GAWTC 3 cut(s) 113, 266, 832
PflMI CCANNNNNTGG 1 cut(s) 1077
PinAI ACCGGT 1 cut(s) 1074
PkrI GCNGC 4 cut(s) 227, 230, 233, 810
PsiI TTATAA 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 679
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 4 cut(s) 367, 534, 678, 1069
PspPI GGNCC 1 cut(s) 533
PstI CTGCAG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 2 cut(s) 678, 1060
RsaNI GTAC 2 cut(s) 677, 1059
RseI CAYNNNNRTG 4 cut(s) 411, 475, 849, 1147
SaqAI TTAA 2 cut(s) 245, 779
SatI GCNGC 4 cut(s) 226, 229, 232, 809
Sau3AI GATC 3 cut(s) 63, 502, 751
Sau96I GGNCC 1 cut(s) 533
ScrFI CCNGG 1 cut(s) 681
SfaNI GCATC 1 cut(s) 671
SfcI CTRYAG 1 cut(s) 496
SfuI TTCGAA 2 cut(s) 595, 835
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 4 cut(s) 411, 475, 849, 1147
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SphI GCATGC 1 cut(s) 948
Sse9I AATT 8 cut(s) 349, 416, 438, 588, 693, 914, 970, 1105
SsiI CCGC 2 cut(s) 226, 1120
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 679
StyI CCWWGG 1 cut(s) 1086
TaaI ACNGT 9 cut(s) 58, 239, 325, 581, 881, 897, 1049, 1058, 1149
TaiI ACGT 3 cut(s) 39, 315, 890
TaqI TCGA 3 cut(s) 595, 835, 1038
TaqII GACCGA 1 cut(s) 550
TasI AATT 8 cut(s) 349, 416, 438, 588, 693, 914, 970, 1105
TatI WGTACW 1 cut(s) 1058
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 3 cut(s) 113, 266, 832
Tru1I TTAA 2 cut(s) 245, 779
Tru9I TTAA 2 cut(s) 245, 779
TscAI CASTG 5 cut(s) 330, 376, 388, 886, 1061
TseFI GTSAC 1 cut(s) 1140
TseI GCWGC 3 cut(s) 228, 231, 808
Tsp45I GTSAC 1 cut(s) 1140
TspDTI ATGAA 6 cut(s) 47, 200, 247, 333, 429, 833
TspRI CASTG 5 cut(s) 330, 376, 388, 886, 1061
Van91I CCANNNNNTGG 1 cut(s) 1077
VpaK11BI GGWCC 1 cut(s) 533
XapI RAATTY 3 cut(s) 349, 416, 438
XceI RCATGY 2 cut(s) 77, 948
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.