pycom08g16800

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000522
Physical Location & Seq
Forward (+)
16906599 .. 16907845
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGCTTCTAAGTTCTTTCATCTTGTGAAAGTTTCATCATTTCTCATAATCTTCCATTTCCTCAGCTCTCGATCTCTAGCAGATGTCCCTCTAAACACTCCTCTCCCACCAGAAACCATTTGCAAGTCCACCCCACATCCTTCTTACTGCACATCTGTCCTTCCCCACAACAACGAAAGTGTCTATGACTTTGGCAGGTTTTCAGTCCAACGTGCACTTTCCGAATCCCATAAGCTCTTAGATTTGTATGAAAAATATCTTCAAAAGGGTTCAAGTTTAACAAATCCTGCAATCCAAGCACTTGAGGACTGCAAGCAGCTTGCATTGTTAAACATTGATTTCTTATCGAGCTCCCTCGAAACTGTTAACAAAGCGAGTGAAGTTCTTCCTATCTTGGATGCCGATGATGTCCAAACATTGCTTAGCGCAATTCTAACCAACCACCAAACTTGCTCCGATGGGATTGCATCTCTGCCTTCTTCAGCTGGGAGTGTCCTAGGTGATCTATCAGTTCCACTCTCTAACAACACAAAATTGTATAGTACCTCTTTGGCTCTCTTTACCAAGGGTTGGGTGCCTAAGGACAAAAATGGGGTGCCAAAGCAACCCAAGAGGCAGTTCAAATTCGGGAAGGGACGTTTGAACTTGAAAATGTCAACCCATGCTCGTGCAATTTATGACTCCGCTATCAATCATCGGGGAAGAAGGCTACTTCAGGTGGGAGATGAAGAAGTTTTGGTGAAGGACATTGTGGTTGTGAGTCAAGATGGAAGTGGAAACTTCACCACCATCAATCAAGCCATTGCCGTTGCACCAAATAACTCTGTTGCTAGTGGTGGGTACTTCATGATATATATCACTGCCGGTGTTTATGAAGAGTACGTGTCAATTATATCAAAAAAGAAGTATTTGTTGTTCGTCGGAGATGGTATCAATCAGACTATCATCACAGGAAATAACAGCGTAGGAGATGGATCGATCAACTTTCAATTCTGCTACTTTAGCTGTGGTGGCACAAGGGTTTGTGGCAGTAAACATCACTGTTCGTAA

Protein Analysis

350

Amino Acids

38.04

Weight (kDa)

8.52

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 186 4.5e-23 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 251 - 340 7.6e-20 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000522)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02810 AT2G47550 AT4G02330
fragaria_vesca FvH4_2g25960 FvH4_2g25960 FvH4_2g25970 FvH4_5g35570 FvH4_5g35580 FvH4_5g35590
malus_domestica MD03G1290900.v1.1 MD03G1291000.v1.1 MD08G1195600.v1.1 MD11G1307400.v1.1 MD11G1307500.v1.1
prunus_persica Prupe.1G529400_v2.0.a1 Prupe.1G529500_v2.0.a1 Prupe.8G263900_v2.0.a1 Prupe.8G264000_v2.0.a1
pyrus_communis pycom08g16800
rosa_chinensis RchiOBHm_Chr6g0294321 RchiOBHm_Chr6g0294331 RchiOBHm_Chr7g0236951 RchiOBHm_Chr7g0236971 RchiOBHm_Chr7g0237041 RchiOBHm_Chr7g0237051
rosa_laevigata RLG00000001034 RLG00000001035 RLG00000001039 RLG00000001043 RLG00000001045 RLG00000011880 RLG00000011881 RLG00000034181
rosa_multiflora Rmu_co8086188.1_g000001 Rmu_co8458485.1_g000001 Rmu_sc0000272.1_g000007 Rmu_sc0000272.1_g000009 Rmu_sc0002131.1_g000030 Rmu_sc0002131.1_g000037 Rmu_sc0005369.1_g000005 Rmu_sc0005369.1_g000008 Rmu_sc0007991.1_g000005 Rmu_sc0007991.1_g000013 Rmu_sc0033563.1_g000001
rosa_roxburghii Rroxscaffold_3G00224540 Rroxscaffold_3G00224600 Rroxscaffold_3G00224650 Rroxscaffold_3G00224660 Rroxscaffold_7G00173470 Rroxscaffold_7G00173480
rosa_rugosa Rorug06G0243400 Rorug06G0243500 Rorug07G0301200 Rorug07G0301500 Rorug07G0301600
rosa_samantha Rh6AG356000 Rh6AG356100 Rh6BG362600 Rh6BG362800 Rh6CG370000 Rh6CG370100 Rh6DG356500 Rh6DG356600 Rh7AG456200 Rh7AG456500 Rh7AG457500 Rh7AG457600 Rh7BG427500 Rh7BG427700 Rh7BG428400 Rh7BG428500 Rh7CG475100 Rh7CG475300 Rh7CG475700 Rh7CG475800 Rh7DG444100 Rh7DG444300 Rh7DG444700 Rh7DG444900
rosa_wichuraiana Rw6G031020 Rw6G031030 Rw7G037870 Rw7G037880 Rw7G037920 Rw7G037940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 186
AccB1I GGYRCC 2 cut(s) 574, 595
AccB7I CCANNNNNTGG 1 cut(s) 570
AciI CCGC 1 cut(s) 684
AclWI GGATC 1 cut(s) 982
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 465, 698
AfaI GTAC 3 cut(s) 544, 842, 881
AfiI CCNNNNNNNGG 1 cut(s) 570
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 6 cut(s) 263, 273, 622, 643, 649, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 6 cut(s) 66, 235, 319, 351, 485, 1005
AluI AGCT 6 cut(s) 66, 235, 319, 351, 485, 1005
Alw21I GWGCWC 2 cut(s) 217, 353
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 1 cut(s) 982
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 1 cut(s) 623
Asp700I GAANNNNTTC 1 cut(s) 384
AspA2I CCTAGG 1 cut(s) 496
AspLEI GCGC 1 cut(s) 428
AsuHPI GGTGA 3 cut(s) 512, 751, 775
AvrII CCTAGG 1 cut(s) 496
AxyI CCTNAGG 1 cut(s) 579
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 574, 595
BanII GRGCYC 1 cut(s) 353
BauI CACGAG 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 217, 353
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 328
BccI CCATC 5 cut(s) 452, 761, 797, 920, 965
BceAI ACGGC 1 cut(s) 791
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BfaI CTAG 3 cut(s) 77, 497, 831
BfuAI ACCTGC 1 cut(s) 186
BisI GCNGC 1 cut(s) 317
BlnI CCTAGG 1 cut(s) 496
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 1 cut(s) 318
BmiI GGNNCC 2 cut(s) 576, 597
BmsI GCATC 2 cut(s) 388, 476
Bpu10I CCTNAGC 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 977
BsaAI YACGTR 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 854
BsaJI CCNNGG 2 cut(s) 496, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 570
Bse118I RCCGGY 1 cut(s) 863
Bse21I CCTNAGG 1 cut(s) 579
Bse3DI GCAATG 2 cut(s) 416, 801
Bse8I GATNNNNATC 1 cut(s) 854
BseCI ATCGAT 1 cut(s) 977
BseDI CCNNGG 2 cut(s) 496, 564
BseGI GGATG 2 cut(s) 136, 403
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 1 cut(s) 570
BseMI GCAATG 2 cut(s) 416, 801
BseMII CTCAG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 1 cut(s) 328
BseYI CCCAGC 1 cut(s) 485
BsgI GTGCAG 1 cut(s) 133
BshNI GGYRCC 2 cut(s) 574, 595
BshVI ATCGAT 1 cut(s) 977
BsiHKAI GWGCWC 2 cut(s) 217, 353
BsiSI CCGG 1 cut(s) 864
BslFI GGGAC 2 cut(s) 71, 648
BslI CCNNNNNNNGG 1 cut(s) 570
BsmFI GGGAC 2 cut(s) 71, 648
Bsp1286I GDGCHC 2 cut(s) 217, 353
Bsp143I GATC 4 cut(s) 71, 502, 974, 978
Bsp1720I GCTNAGC 1 cut(s) 422
BspACI CCGC 1 cut(s) 684
BspCNI CTCAG 1 cut(s) 75
BspDI ATCGAT 1 cut(s) 977
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 2 cut(s) 576, 597
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 982
BspT107I GGYRCC 2 cut(s) 574, 595
BsrDI GCAATG 2 cut(s) 416, 801
BsrFI RCCGGY 1 cut(s) 863
BssAI RCCGGY 1 cut(s) 863
BssECI CCNNGG 2 cut(s) 496, 564
BssMI GATC 4 cut(s) 71, 502, 974, 978
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 2 cut(s) 496, 564
Bst2BI CACGAG 1 cut(s) 666
Bst4CI ACNGT 2 cut(s) 364, 1043
Bst6I CTCTTC 1 cut(s) 870
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 2 cut(s) 314, 321
BstDEI CTNAG 5 cut(s) 9, 62, 238, 422, 579
BstF5I GGATG 2 cut(s) 136, 403
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 4 cut(s) 74, 505, 977, 981
BstMBI GATC 4 cut(s) 71, 502, 974, 978
BstMWI GCNNNNNNNGC 3 cut(s) 296, 1002, 1011
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 977
Bsu36I CCTNAGG 1 cut(s) 579
BsuTUI ATCGAT 1 cut(s) 977
BtsCI GGATG 2 cut(s) 136, 403
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 2 cut(s) 858, 1039
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 314, 321
CciI TCATGA 1 cut(s) 846
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 863
ClaI ATCGAT 1 cut(s) 977
Csp6I GTAC 3 cut(s) 543, 841, 880
CspCI CAANNNNNGTGG 2 cut(s) 431, 466
CviAII CATG 2 cut(s) 662, 847
CviQI GTAC 3 cut(s) 543, 841, 880
DdeI CTNAG 5 cut(s) 9, 62, 238, 422, 579
DpnI GATC 4 cut(s) 73, 504, 976, 980
DpnII GATC 4 cut(s) 71, 502, 974, 978
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 496, 564
Eco24I GRGCYC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 2 cut(s) 465, 698
Eco81I CCTNAGG 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 351
EcoT14I CCWWGG 2 cut(s) 496, 564
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 2 cut(s) 496, 564
FaeI CATG 2 cut(s) 665, 850
FaqI GGGAC 2 cut(s) 71, 648
FatI CATG 2 cut(s) 661, 846
Fnu4HI GCNGC 1 cut(s) 317
FokI GGATG 2 cut(s) 123, 410
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 317
FspBI CTAG 3 cut(s) 77, 497, 831
GlaI GCGC 1 cut(s) 427
GluI GCNGC 1 cut(s) 317
GsaI CCCAGC 1 cut(s) 489
HapII CCGG 1 cut(s) 864
HhaI GCGC 1 cut(s) 428
Hin1II CATG 2 cut(s) 665, 850
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 2 cut(s) 367, 657
HindII GTYRAC 2 cut(s) 367, 657
HinfI GANTC 3 cut(s) 224, 680, 760
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 864
HphI GGTGA 3 cut(s) 512, 751, 775
Hpy166II GTNNAC 5 cut(s) 129, 215, 367, 657, 1034
Hpy188I TCNGA 4 cut(s) 223, 457, 923, 939
Hpy188III TCNNGA 4 cut(s) 69, 628, 764, 847
Hpy8I GTNNAC 5 cut(s) 129, 215, 367, 657, 1034
Hpy99I CGWCG 1 cut(s) 923
HpyAV CCTTC 6 cut(s) 150, 170, 486, 625, 699, 736
HpyCH4III ACNGT 2 cut(s) 364, 1043
HpyCH4IV ACGT 3 cut(s) 211, 637, 882
HpyCH4V TGCA 9 cut(s) 123, 150, 215, 290, 312, 323, 467, 671, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 1002, 1011
HpyF3I CTNAG 5 cut(s) 9, 62, 238, 422, 579
HpySE526I ACGT 3 cut(s) 211, 637, 882
Hsp92II CATG 2 cut(s) 665, 850
HspAI GCGC 1 cut(s) 426
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 4 cut(s) 71, 502, 974, 978
LmnI GCTCC 2 cut(s) 356, 458
LpnPI CCDG 7 cut(s) 123, 181, 300, 471, 701, 877, 936
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 2 cut(s) 388, 476
MaeI CTAG 3 cut(s) 77, 497, 831
MaeII ACGT 3 cut(s) 211, 637, 882
MalI GATC 4 cut(s) 73, 504, 976, 980
MboI GATC 4 cut(s) 71, 502, 974, 978
MboII GAAGA 7 cut(s) 43, 251, 377, 471, 714, 740, 887
MhlI GDGCHC 2 cut(s) 217, 353
MluCI AATT 6 cut(s) 429, 533, 623, 672, 888, 989
MlyI GAGTC 2 cut(s) 674, 769
MmeI TCCRAC 2 cut(s) 232, 901
MnlI CCTC 7 cut(s) 71, 99, 111, 298, 365, 556, 606
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 3 cut(s) 278, 329, 366
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 864
MwoI GCNNNNNNNGC 3 cut(s) 296, 1002, 1011
NdeII GATC 4 cut(s) 71, 502, 974, 978
NlaIII CATG 2 cut(s) 665, 850
NlaIV GGNNCC 2 cut(s) 576, 597
PagI TCATGA 1 cut(s) 846
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 570
PkrI GCNGC 1 cut(s) 318
PleI GAGTC 2 cut(s) 674, 768
PpsI GAGTC 2 cut(s) 674, 768
Ppu21I YACGTR 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 485
PspN4I GGNNCC 2 cut(s) 576, 597
PsrI GAACNNNNNNTAC 2 cut(s) 899, 931
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 3 cut(s) 544, 842, 881
RsaNI GTAC 3 cut(s) 543, 841, 880
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 278, 329, 366
SatI GCNGC 1 cut(s) 317
Sau3AI GATC 4 cut(s) 71, 502, 974, 978
SchI GAGTC 2 cut(s) 674, 769
SduI GDGCHC 2 cut(s) 217, 353
SfaNI GCATC 2 cut(s) 388, 476
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 6 cut(s) 429, 533, 623, 672, 888, 989
SsiI CCGC 1 cut(s) 684
SspMI CTAG 3 cut(s) 77, 497, 831
SstI GAGCTC 1 cut(s) 353
StyI CCWWGG 2 cut(s) 496, 564
TaaI ACNGT 2 cut(s) 364, 1043
TaiI ACGT 3 cut(s) 214, 640, 885
TaqI TCGA 4 cut(s) 70, 347, 357, 977
TasI AATT 6 cut(s) 429, 533, 623, 672, 888, 989
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 3 cut(s) 278, 329, 366
Tru9I TTAA 3 cut(s) 278, 329, 366
TscAI CASTG 2 cut(s) 865, 1046
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 6 cut(s) 8, 24, 264, 741, 835, 888
TspRI CASTG 2 cut(s) 865, 1046
Van91I CCANNNNNTGG 1 cut(s) 570
VneI GTGCAC 1 cut(s) 213
XapI RAATTY 1 cut(s) 623
XmaJI CCTAGG 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 3 cut(s) 77, 497, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.