pycom10g20400

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001644
Physical Location & Seq
Forward (+)
23292265 .. 23294556
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1866 bp
ATGAGATTCCTTAAACGCCAAATCTGTGCTGGCGTCCAGGCTATCAAAACCCCCCCACCCTCGGTAGAAGAAGCCACCCAACGGCTCTCGTCCTATGCGGTGGCTTTACAGTTTTCGAAAATATATAGCCGTTACAACCCAAGCAAAAGCCCACAACTCTCTCCTCTCTTAAACGCCACTAGTTCGCACCAGAGTGCAAGCAAATTTTCTCTCTTCCCAAACAGAGCAACCAATTTTCTCTCCAGTTTTCTGGGATTTTCTTGCATTTTCTCGGGTGATTCGCTATTCGGGTTGATCGAGAAGATGACTGTGGAGGAGACCCAGGTGGCTGAAGTGGTGGTTGTTCCTCAGGAGGAGGCGGAGAAGGTTGTGTGTGAGCATGAGAAGGTTGTTGAGGGAGATGATAAGGTGAAGGAGGTGGATGAGGCGGAGTCGAAGCCGAAAACGGTTGAGAGGAGCTCTTCTTATAAGGAAGAGAGCAACTATCTCTCCGATTTGAAGGAGTTCGAGAAGAAGGCGTTGAACGAGCTGAAATCGAAGCTGGAGGAGGCCATTTTGGGCAACAATATCTTCAAGAAGGAGAAGCGGAAGAAGGAGGAGCAGAAGGAAGAGAAAAAAGTAGCAGAGGAGGAAGAGAAGAAGGAAGAGGAGGAAGAGAAGAAGGAAGAGAAAATAGAGGAGGAAGTGAAAACGAAGGAGGAAGAGAAGAATGAAGAGAAAGCAGAGGAGGTTTGTGAGACACAGACAGAGGAAGTAGCCAAAGAGAAAGCCGAAGAAGGCAAACAAGAGAAGAAATCGGAAGATGGGTATGAAGTAGTAGACACGGATATCTCTCTTTGGGGAGTGCCCCTATTCCCTGGGAAGGGTTTTGAGGGCACTGATGTTGTGCTCTTAAAGTTCTTGAGGGCTAGGGAGTTCAAGGTCAATGAGGCTTTCGAGATGCTGAAGAAAACCCTTCAATGGAGGAAGGAGTCGAAGATCGATTCGATCTTGGACGAGGATGTCTGTGCTGATTTGAGCTCTGCTGCTTACTTGAACGGCGTTGATCGCGAAGGCCACCCCGTTTGCTACAACATTTTTGGGGTTTTCGACAACGAGGAGCTTTATCGGAAGACTTTTGGGAATGAGGAGAAGAGAGGGCAGTTCCTGAGGTGGAGGCTCCAGCTGATGGAGAAGAGCATCCAGAAGCTGGATTTGAGGCCTGGTGGTGTCACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCCGGGCCTTCGAAGAAGGAGCTCAGGATTGCCACAAAGCAAGCTGTTGGGCTTTTGCAGGACAACTACCCTGAGTTGGTTGCCAAAAATATCTTCATAAACGCTCCTTTCTGGTACTATGCACTCAATGCTTTGCTATCTCCTTTCTTGACTCAAAGAACCAAGAGCAAGTTTGTCGTTGCTCGCCCGGCGAAGGTCATTGAAACCCTTCTCAAGTACATTCCAGCTCAGGAGATACCTGCTCAGTATGGCGGCTTCAAGAGGGACAACGACAGCGAGTTCTCTGCCGAAGATGGCGCCGTTTCGGAACTTATCATCAAGGCGGGATCCACTGGAACCGTACAGATTGATGCAGCAGAGGTTGATAACACATTAGTGTGGGACTTGACTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCCACTGATGAGGGGTCATACACCATTATTGTACAGAAGAAGAAGAAAATGGGGTCTAATGAAGGCCCGATTCGCAACACTTTCCGAAGCAACGAACCTGGGAAGGTTGTCCTGACAATCGAGAACAATTCGAGCAAGAAGAAGAGGGTTCTGCACCGGTACAAGGCCAAGAAGTGCTCCACATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

622

Amino Acids

70.65

Weight (kDa)

5.44

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 262 - 317 1.2e-06 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 342 - 501 9.8e-24 CRAL/TRIO domain
GOLD_PATL1_C PF25099 523 - 607 2.5e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AasI GACNNNNNNGTC 1 cut(s) 1001
Acc36I ACCTGC 1 cut(s) 1495
AccB1I GGYRCC 1 cut(s) 1544
AccB7I CCANNNNNTGG 2 cut(s) 1168, 1189
AccI GTMKAC 1 cut(s) 819
AccII CGCG 1 cut(s) 1050
AciI CCGC 6 cut(s) 98, 359, 428, 586, 1500, 1571
AclWI GGATC 2 cut(s) 1569, 1582
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 3 cut(s) 351, 965, 1208
AcyI GRCGYC 2 cut(s) 33, 1545
AfaI GTAC 5 cut(s) 1364, 1466, 1590, 1710, 1838
AgeI ACCGGT 1 cut(s) 1833
AgsI TTSAA 8 cut(s) 499, 523, 574, 919, 959, 1036, 1451, 1507
AhlI ACTAGT 1 cut(s) 179
AjnI CCWGG 5 cut(s) 36, 321, 856, 1201, 1774
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AleI CACNNNNGTG 2 cut(s) 192, 1622
Alw21I GWGCWC 5 cut(s) 461, 891, 1022, 1272, 1856
Alw26I GTCTC 2 cut(s) 311, 731
AlwI GGATC 2 cut(s) 1569, 1582
AlwNI CAGNNNCTG 2 cut(s) 1147, 1189
Ama87I CYCGRG 2 cut(s) 271, 1250
AoxI GGCC 6 cut(s) 549, 1054, 1199, 1253, 1741, 1842
ApeKI GCWGC 2 cut(s) 1025, 1601
ApoI RAATTY 1 cut(s) 203
AsiGI ACCGGT 1 cut(s) 1833
Asp700I GAANNNNTTC 2 cut(s) 503, 1455
AspLEI GCGC 1 cut(s) 1547
AspS9I GGNCC 2 cut(s) 1253, 1742
AsuC2I CCSGG 3 cut(s) 1251, 1252, 1436
AsuHPI GGTGA 2 cut(s) 287, 421
AsuII TTCGAA 2 cut(s) 116, 1259
AvaI CYCGRG 2 cut(s) 271, 1250
AxyI CCTNAGG 2 cut(s) 348, 1148
BaeGI GKGCMC 2 cut(s) 849, 878
BamHI GGATCC 1 cut(s) 1574
BanI GGYRCC 1 cut(s) 1544
BanII GRGCYC 3 cut(s) 461, 1022, 1272
BarI GAAGNNNNNNTAC 2 cut(s) 1487, 1519
BbsI GAAGAC 1 cut(s) 1118
Bbv12I GWGCWC 5 cut(s) 461, 891, 1022, 1272, 1856
BbvI GCAGC 2 cut(s) 1012, 1613
BccI CCATC 4 cut(s) 797, 1162, 1535, 1641
BceAI ACGGC 4 cut(s) 98, 114, 1054, 1532
BcgI CGANNNNNNTGC 4 cut(s) 1405, 1439, 1514, 1548
BciT130I CCWGG 5 cut(s) 38, 323, 858, 1203, 1776
BcnI CCSGG 3 cut(s) 1251, 1252, 1436
BcoDI GTCTC 2 cut(s) 311, 731
BcuI ACTAGT 1 cut(s) 179
BfaI CTAG 2 cut(s) 180, 909
BfoI RGCGCY 1 cut(s) 1548
BfuAI ACCTGC 1 cut(s) 1495
BisI GCNGC 3 cut(s) 1026, 1501, 1602
BlsI GCNGC 3 cut(s) 1027, 1502, 1603
Bme1390I CCNGG 8 cut(s) 38, 323, 858, 1203, 1251, 1252, 1436, 1776
BmeT110I CYCGRG 2 cut(s) 271, 1250
BmgT120I GGNCC 2 cut(s) 1253, 1742
BmiI GGNNCC 4 cut(s) 1160, 1546, 1576, 1585
BmrFI CCNGG 8 cut(s) 38, 323, 858, 1203, 1251, 1252, 1436, 1776
BmsI GCATC 3 cut(s) 930, 1188, 1588
BpiI GAAGAC 1 cut(s) 1118
BplI GAGNNNNNCTC 2 cut(s) 443, 475
BpmI CTGGAG 3 cut(s) 226, 563, 1145
Bpu10I CCTNAGC 2 cut(s) 1271, 1476
Bpu14I TTCGAA 2 cut(s) 116, 1259
BpuEI CTTGAG 3 cut(s) 922, 1223, 1445
BpuMI CCSGG 3 cut(s) 1251, 1252, 1436
Bsa29I ATCGAT 1 cut(s) 981
BsaHI GRCGYC 2 cut(s) 33, 1545
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 6 cut(s) 60, 321, 856, 857, 1250, 1775
BsaWI WCCGGW 1 cut(s) 1833
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bse118I RCCGGY 1 cut(s) 1833
Bse1I ACTGG 2 cut(s) 243, 1585
Bse21I CCTNAGG 2 cut(s) 348, 1148
BseBI CCWGG 5 cut(s) 38, 323, 858, 1203, 1776
BseCI ATCGAT 1 cut(s) 981
BseDI CCNNGG 6 cut(s) 60, 321, 856, 857, 1250, 1775
BseGI GGATG 4 cut(s) 427, 1006, 1179, 1652
BseMII CTCAG 6 cut(s) 362, 1139, 1285, 1311, 1490, 1505
BseNI ACTGG 2 cut(s) 243, 1585
BseSI GKGCMC 2 cut(s) 849, 878
BseXI GCAGC 2 cut(s) 1012, 1613
BsgI GTGCAG 1 cut(s) 1814
Bsh1236I CGCG 1 cut(s) 1050
BshFI GGCC 6 cut(s) 551, 1056, 1201, 1255, 1743, 1844
BshNI GGYRCC 1 cut(s) 1544
BshTI ACCGGT 1 cut(s) 1833
BshVI ATCGAT 1 cut(s) 981
BsiHKAI GWGCWC 5 cut(s) 461, 891, 1022, 1272, 1856
BsiHKCI CYCGRG 2 cut(s) 271, 1250
BsiSI CCGG 3 cut(s) 1251, 1436, 1834
BslFI GGGAC 2 cut(s) 1526, 1643
BsmAI GTCTC 2 cut(s) 311, 731
BsmFI GGGAC 2 cut(s) 1526, 1643
BsnI GGCC 6 cut(s) 551, 1056, 1201, 1255, 1743, 1844
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 2 cut(s) 271, 1250
Bsp119I TTCGAA 2 cut(s) 116, 1259
Bsp1286I GDGCHC 7 cut(s) 461, 849, 878, 891, 1022, 1272, 1856
Bsp1407I TGTACA 1 cut(s) 1708
Bsp143I GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
Bsp68I TCGCGA 1 cut(s) 1050
BspACI CCGC 6 cut(s) 98, 359, 428, 586, 1500, 1571
BspANI GGCC 6 cut(s) 551, 1056, 1201, 1255, 1743, 1844
BspCNI CTCAG 6 cut(s) 361, 1140, 1284, 1312, 1489, 1504
BspDI ATCGAT 1 cut(s) 981
BspFNI CGCG 1 cut(s) 1050
BspLI GGNNCC 4 cut(s) 1160, 1546, 1576, 1585
BspMI ACCTGC 1 cut(s) 1495
BspPI GGATC 2 cut(s) 1569, 1582
BspQI GCTCTTC 2 cut(s) 466, 1169
BspT104I TTCGAA 2 cut(s) 116, 1259
BspT107I GGYRCC 1 cut(s) 1544
BspTNI GGTCTC 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 1833
BsrGI TGTACA 1 cut(s) 1708
BsrI ACTGG 2 cut(s) 243, 1585
BssAI RCCGGY 1 cut(s) 1833
BssECI CCNNGG 6 cut(s) 60, 321, 856, 857, 1250, 1775
BssMI GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
BssNI GRCGYC 2 cut(s) 33, 1545
Bst2UI CCWGG 5 cut(s) 38, 323, 858, 1203, 1776
Bst4CI ACNGT 5 cut(s) 111, 310, 448, 1588, 1639
BstACI GRCGYC 2 cut(s) 33, 1545
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstAUI TGTACA 1 cut(s) 1708
BstBI TTCGAA 2 cut(s) 116, 1259
BstC8I GCNNGC 4 cut(s) 31, 199, 1290, 1432
BstDEI CTNAG 6 cut(s) 348, 1148, 1271, 1320, 1476, 1491
BstF5I GGATG 4 cut(s) 427, 1006, 1179, 1652
BstFNI CGCG 1 cut(s) 1050
BstH2I RGCGCY 1 cut(s) 1548
BstHHI GCGC 1 cut(s) 1547
BstKTI GATC 6 cut(s) 297, 981, 990, 1048, 1230, 1577
BstMAI GTCTC 2 cut(s) 311, 731
BstMBI GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
BstMWI GCNNNNNNNGC 4 cut(s) 1047, 1376, 1436, 1749
BstNI CCWGG 5 cut(s) 38, 323, 858, 1203, 1776
BstSCI CCNGG 8 cut(s) 36, 321, 856, 1201, 1249, 1250, 1434, 1774
BstSLI GKGCMC 2 cut(s) 849, 878
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 2 cut(s) 1012, 1613
BstV2I GAAGAC 1 cut(s) 1118
BstX2I RGATCY 1 cut(s) 1574
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 1 cut(s) 1574
Bsu15I ATCGAT 1 cut(s) 981
Bsu36I CCTNAGG 2 cut(s) 348, 1148
BsuRI GGCC 6 cut(s) 551, 1056, 1201, 1255, 1743, 1844
BsuTUI ATCGAT 1 cut(s) 981
BtsCI GGATG 4 cut(s) 427, 1006, 1179, 1652
BtsIMutI CAGTG 3 cut(s) 876, 1578, 1680
BtuMI TCGCGA 1 cut(s) 1050
BveI ACCTGC 1 cut(s) 1495
Cac8I GCNNGC 4 cut(s) 31, 199, 1290, 1432
CaiI CAGNNNCTG 2 cut(s) 1147, 1189
CfoI GCGC 1 cut(s) 1547
Cfr10I RCCGGY 1 cut(s) 1833
Cfr13I GGNCC 2 cut(s) 1253, 1742
Cfr9I CCCGGG 1 cut(s) 1250
ClaI ATCGAT 1 cut(s) 981
CseI GACGC 1 cut(s) 22
Csp6I GTAC 5 cut(s) 1363, 1465, 1589, 1709, 1837
CspAI ACCGGT 1 cut(s) 1833
CspCI CAANNNNNGTGG 2 cut(s) 1046, 1081
CviAII CATG 1 cut(s) 380
CviQI GTAC 5 cut(s) 1363, 1465, 1589, 1709, 1837
DdeI CTNAG 6 cut(s) 348, 1148, 1271, 1320, 1476, 1491
DinI GGCGCC 1 cut(s) 1546
DpnI GATC 6 cut(s) 296, 980, 989, 1047, 1229, 1576
DpnII GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
DrdI GACNNNNNNGTC 1 cut(s) 1001
DseDI GACNNNNNNGTC 1 cut(s) 1001
EciI GGCGGA 2 cut(s) 374, 443
Ecl136II GAGCTC 3 cut(s) 459, 1020, 1270
Eco147I AGGCCT 1 cut(s) 1201
Eco24I GRGCYC 3 cut(s) 461, 1022, 1272
Eco31I GGTCTC 1 cut(s) 311
Eco32I GATATC 1 cut(s) 829
Eco53kI GAGCTC 3 cut(s) 459, 1020, 1270
Eco57I CTGAAG 3 cut(s) 351, 965, 1208
Eco81I CCTNAGG 2 cut(s) 348, 1148
Eco88I CYCGRG 2 cut(s) 271, 1250
EcoICRI GAGCTC 3 cut(s) 459, 1020, 1270
EcoRII CCWGG 5 cut(s) 36, 321, 856, 1201, 1774
EcoRV GATATC 1 cut(s) 829
EcoT38I GRGCYC 3 cut(s) 461, 1022, 1272
EgeI GGCGCC 1 cut(s) 1546
EheI GGCGCC 1 cut(s) 1546
FaeI CATG 1 cut(s) 383
FaqI GGGAC 2 cut(s) 1526, 1643
FatI CATG 1 cut(s) 379
FauI CCCGC 1 cut(s) 1564
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 3 cut(s) 1026, 1501, 1602
FokI GGATG 4 cut(s) 434, 1013, 1166, 1659
FriOI GRGCYC 3 cut(s) 461, 1022, 1272
Fsp4HI GCNGC 3 cut(s) 1026, 1501, 1602
FspBI CTAG 2 cut(s) 180, 909
GlaI GCGC 1 cut(s) 1546
GluI GCNGC 3 cut(s) 1026, 1501, 1602
GsuI CTGGAG 3 cut(s) 226, 563, 1145
HaeII RGCGCY 1 cut(s) 1548
HaeIII GGCC 6 cut(s) 551, 1056, 1201, 1255, 1743, 1844
HapII CCGG 3 cut(s) 1251, 1436, 1834
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 1547
Hin1I GRCGYC 2 cut(s) 33, 1545
Hin1II CATG 1 cut(s) 383
Hin6I GCGC 1 cut(s) 1545
HinP1I GCGC 1 cut(s) 1545
HinfI GANTC 7 cut(s) 6, 278, 431, 971, 983, 1399, 1747
HpaII CCGG 3 cut(s) 1251, 1436, 1834
HphI GGTGA 2 cut(s) 287, 421
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 7 cut(s) 493, 799, 1110, 1227, 1555, 1763, 1865
Hpy8I GTNNAC 1 cut(s) 820
HpyCH4III ACNGT 5 cut(s) 111, 310, 448, 1588, 1639
HpyCH4V TGCA 6 cut(s) 197, 264, 1306, 1370, 1601, 1831
HpyF10VI GCNNNNNNNGC 4 cut(s) 1047, 1376, 1436, 1749
HpyF3I CTNAG 6 cut(s) 348, 1148, 1271, 1320, 1476, 1491
Hsp92I GRCGYC 2 cut(s) 33, 1545
Hsp92II CATG 1 cut(s) 383
HspAI GCGC 1 cut(s) 1545
KasI GGCGCC 1 cut(s) 1544
Kzo9I GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
LguI GCTCTTC 2 cut(s) 466, 1169
LmnI GCTCC 7 cut(s) 456, 598, 1099, 1164, 1267, 1357, 1859
Lsp1109I GCAGC 2 cut(s) 1012, 1613
LweI GCATC 3 cut(s) 930, 1188, 1588
MaeI CTAG 2 cut(s) 180, 909
MaeIII GTNAC 2 cut(s) 131, 1210
MalI GATC 6 cut(s) 296, 980, 989, 1047, 1229, 1576
MboI GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
MflI RGATCY 1 cut(s) 1574
MhlI GDGCHC 7 cut(s) 461, 849, 878, 891, 1022, 1272, 1856
MluCI AATT 4 cut(s) 203, 232, 1657, 1804
Mly113I GGCGCC 1 cut(s) 1545
MlyI GAGTC 3 cut(s) 440, 980, 1393
MroXI GAANNNNTTC 2 cut(s) 503, 1455
MseI TTAA 3 cut(s) 12, 170, 893
MslI CAYNNNNRTG 3 cut(s) 192, 1622, 1624
MspA1I CMGCKG 1 cut(s) 1165
MspI CCGG 3 cut(s) 1251, 1436, 1834
MspR9I CCNGG 8 cut(s) 38, 323, 858, 1203, 1251, 1252, 1436, 1776
MvaI CCWGG 5 cut(s) 38, 323, 858, 1203, 1776
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 4 cut(s) 1047, 1376, 1436, 1749
NarI GGCGCC 1 cut(s) 1545
NciI CCSGG 3 cut(s) 1251, 1252, 1436
NdeII GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
NlaIII CATG 1 cut(s) 383
NlaIV GGNNCC 4 cut(s) 1160, 1546, 1576, 1585
NmuCI GTSAC 1 cut(s) 1210
NruI TCGCGA 1 cut(s) 1050
NspV TTCGAA 2 cut(s) 116, 1259
OliI CACNNNNGTG 2 cut(s) 192, 1622
PasI CCCWGGG 1 cut(s) 857
PceI AGGCCT 1 cut(s) 1201
PciSI GCTCTTC 2 cut(s) 466, 1169
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdmI GAANNNNTTC 2 cut(s) 503, 1455
PfeI GAWTC 4 cut(s) 6, 278, 983, 1747
PflMI CCANNNNNTGG 2 cut(s) 1168, 1189
PinAI ACCGGT 1 cut(s) 1833
PkrI GCNGC 3 cut(s) 1027, 1502, 1603
PleI GAGTC 3 cut(s) 439, 979, 1393
PluTI GGCGCC 1 cut(s) 1548
PpsI GAGTC 3 cut(s) 439, 979, 1393
PsiI TTATAA 1 cut(s) 468
Psp124BI GAGCTC 3 cut(s) 461, 1022, 1272
Psp6I CCWGG 5 cut(s) 36, 321, 856, 1201, 1774
PspGI CCWGG 5 cut(s) 36, 321, 856, 1201, 1774
PspN4I GGNNCC 4 cut(s) 1160, 1546, 1576, 1585
PspPI GGNCC 2 cut(s) 1253, 1742
PstNI CAGNNNCTG 2 cut(s) 1147, 1189
PsuI RGATCY 1 cut(s) 1574
PvuII CAGCTG 1 cut(s) 1165
RruI TCGCGA 1 cut(s) 1050
RsaI GTAC 5 cut(s) 1364, 1466, 1590, 1710, 1838
RsaNI GTAC 5 cut(s) 1363, 1465, 1589, 1709, 1837
RseI CAYNNNNRTG 3 cut(s) 192, 1622, 1624
SacI GAGCTC 3 cut(s) 461, 1022, 1272
SapI GCTCTTC 2 cut(s) 466, 1169
SaqAI TTAA 3 cut(s) 12, 170, 893
SatI GCNGC 3 cut(s) 1026, 1501, 1602
Sau3AI GATC 6 cut(s) 294, 978, 987, 1045, 1227, 1574
Sau96I GGNCC 2 cut(s) 1253, 1742
SchI GAGTC 3 cut(s) 440, 980, 1393
ScrFI CCNGG 8 cut(s) 38, 323, 858, 1203, 1251, 1252, 1436, 1776
SduI GDGCHC 7 cut(s) 461, 849, 878, 891, 1022, 1272, 1856
SfaNI GCATC 3 cut(s) 930, 1188, 1588
SfoI GGCGCC 1 cut(s) 1546
SfuI TTCGAA 2 cut(s) 116, 1259
SmaI CCCGGG 1 cut(s) 1252
SmiMI CAYNNNNRTG 3 cut(s) 192, 1622, 1624
SmlI CTYRAG 3 cut(s) 901, 1238, 1460
SmoI CTYRAG 3 cut(s) 901, 1238, 1460
SpeI ACTAGT 1 cut(s) 179
SrfI GCCCGGGC 1 cut(s) 1252
Sse9I AATT 4 cut(s) 203, 232, 1657, 1804
SseBI AGGCCT 1 cut(s) 1201
SsiI CCGC 6 cut(s) 98, 359, 428, 586, 1500, 1571
SspDI GGCGCC 1 cut(s) 1544
SspMI CTAG 2 cut(s) 180, 909
SstI GAGCTC 3 cut(s) 461, 1022, 1272
StuI AGGCCT 1 cut(s) 1201
StyD4I CCNGG 8 cut(s) 36, 321, 856, 1201, 1249, 1250, 1434, 1774
TaaI ACNGT 5 cut(s) 111, 310, 448, 1588, 1639
TasI AATT 4 cut(s) 203, 232, 1657, 1804
TatI WGTACW 2 cut(s) 1464, 1708
TauI GCSGC 1 cut(s) 1503
TfiI GAWTC 4 cut(s) 6, 278, 983, 1747
Tru1I TTAA 3 cut(s) 12, 170, 893
Tru9I TTAA 3 cut(s) 12, 170, 893
TscAI CASTG 3 cut(s) 883, 1585, 1687
TseFI GTSAC 1 cut(s) 1210
TseI GCWGC 2 cut(s) 1025, 1601
Tsp45I GTSAC 1 cut(s) 1210
TspDTI ATGAA 4 cut(s) 726, 825, 1333, 1752
TspGWI ACGGA 1 cut(s) 839
TspMI CCCGGG 1 cut(s) 1250
TspRI CASTG 3 cut(s) 883, 1585, 1687
Van91I CCANNNNNTGG 2 cut(s) 1168, 1189
XapI RAATTY 1 cut(s) 203
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmaI CCCGGG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 819
XmnI GAANNNNTTC 2 cut(s) 503, 1455
XspI CTAG 2 cut(s) 180, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.