pycom10g23610

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001486
Physical Location & Seq
Reverse (-)
25695356 .. 25699568
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGGCTTGGTATTTTGGGGCAAGTTTGACCATGAACGTCCTCCTCCTGCTCCTTACAGTTGTTATCATCCCGCATGCGAACTGTTGCTACACATCTATCATTGGGTTTGGGGATTCACTTGTTGATACTGGCAACATTTACATCGATGCCCGTTACCACCATAAGGGTCCGTACCATTTCTTCTTCCCTCCATATGGGGAGACCTACTTTCATCACCCCACCGGGCGCTGCTGCGACGGTCGTTTAATTATAGACCTCATTGCTGAATCTATGGGGCTTCCACTAGTACGACCTTATCTTGAAGTCCTGAACGTCAATGAAACGAAGCGCAACCTTGAAGAGGGTGTGAATTTTGCAGTTGTTGGAGCCACTGCACTTGACACTGACTTTCTTGACCCAATGGGACGTTATGATGTATTTACAAACTTCTCTCTAAGAATTCAAGTGGATTGGTTCAAAGGAATGCTATCATCTTTATGCAATACATCTTCAGACTGCAGGAATGTCCTTTCAAGTTCTCTCATTGTTATGGGACCGATTGGAGGCAACGATTATAACTGTCCGCTTTTCGCGGGAGAGAGTTTTGAAATTGTTGAAACATATGTTCCTTTCGTGATAGAAGCAATGGCTTCAGCCATCAATGAGTTGATTCAGCATGGGGCATCGACACTTTTGGTACCAGGCAACTTTCCAATTGGTTGCATTCCTGCTTATCTTACCTTATATGAGGGTTCAGATGAGAGTGAATATGACCCGTTAACTGACTATCTTACTTGGTTAAACAAGTTTTCTGAGTATCACAATGAGCTACTTAAGACAAAGTTAAGTCAGATTCGAAGCCTTCATCCTCATGTCAACATCATTTATGGAGATGTTTACAGTGCTGAGCTACAATTGTATCAGTCTCCAGAACAATTTGGATTTACAGAGAATACTTCCAGAGCATGCTGTGGAGGAGGAGGGACATACAATTACAACTTATCAGCACCATGTGGTCTTCCAGGAGCAAGTGCTTGTGCAAACCCTGCAAAGTCTATCAACTGGGACGGTCGGCACACTACTGAAGCAGCAAACCAGTGGATAGCCAAGGCCTTATTAAATGGAATTTACACCATTCCCCACATTAGTACCTCCTGTGTTTCACAACAGTGA

Protein Analysis

384

Amino Acids

42.35

Weight (kDa)

4.93

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 365 7.7e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
Acc65I GGTACC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 676
AccII CGCG 1 cut(s) 572
AciI CCGC 3 cut(s) 71, 563, 572
AcsI RAATTY 3 cut(s) 349, 438, 1104
AcuI CTGAAG 3 cut(s) 474, 615, 1083
AdeI CACNNNGTG 1 cut(s) 992
AfaI GTAC 4 cut(s) 173, 288, 678, 1129
AfiI CCNNNNNNNGG 4 cut(s) 163, 194, 340, 542
AflII CTTAAG 1 cut(s) 812
AgsI TTSAA 7 cut(s) 302, 338, 443, 457, 513, 587, 596
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 2 cut(s) 679, 1000
AleI CACNNNNGTG 1 cut(s) 1147
AluBI AGCT 2 cut(s) 808, 889
AluI AGCT 2 cut(s) 808, 889
Alw26I GTCTC 2 cut(s) 194, 909
AoxI GGCC 1 cut(s) 1089
ApeKI GCWGC 3 cut(s) 228, 231, 1067
ApoI RAATTY 3 cut(s) 349, 438, 1104
Asp718I GGTACC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 228, 330
AspS9I GGNCC 2 cut(s) 167, 533
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 835
AvaII GGWCC 2 cut(s) 167, 533
BaeI ACNNNNGTAYC 4 cut(s) 881, 914, 1111, 1144
BanI GGYRCC 1 cut(s) 676
BarI GAAGNNNNNNTAC 2 cut(s) 164, 196
BbsI GAAGAC 1 cut(s) 989
BbvI GCAGC 3 cut(s) 215, 218, 1079
BccI CCATC 1 cut(s) 644
BciT130I CCWGG 2 cut(s) 681, 1002
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 194, 909
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 812
BisI GCNGC 3 cut(s) 229, 232, 1068
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 3 cut(s) 230, 233, 1069
Bme1390I CCNGG 3 cut(s) 223, 681, 1002
Bme18I GGWCC 2 cut(s) 167, 533
BmgT120I GGNCC 2 cut(s) 167, 533
BmiI GGNNCC 4 cut(s) 168, 367, 534, 678
BmrFI CCNGG 3 cut(s) 223, 681, 1002
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 2 cut(s) 136, 671
BmuI ACTGGG 1 cut(s) 1051
BpiI GAAGAC 1 cut(s) 989
BpmI CTGGAG 1 cut(s) 891
Bpu1102I GCTNAGC 1 cut(s) 885
Bpu14I TTCGAA 1 cut(s) 835
BpuMI CCSGG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 1086
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 194, 340, 542
Bse1I ACTGG 3 cut(s) 133, 1046, 1075
Bse3DI GCAATG 2 cut(s) 258, 630
BseBI CCWGG 2 cut(s) 681, 1002
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 1086
BseGI GGATG 2 cut(s) 66, 844
BseLI CCNNNNNNNGG 4 cut(s) 163, 194, 340, 542
BseMI GCAATG 2 cut(s) 258, 630
BseMII CTCAG 2 cut(s) 783, 876
BseNI ACTGG 3 cut(s) 133, 1046, 1075
BseRI GAGGAG 3 cut(s) 32, 969, 972
BseXI GCAGC 3 cut(s) 215, 218, 1079
BsgI GTGCAG 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 572
Bsh1285I CGRYCG 2 cut(s) 241, 1051
BshFI GGCC 1 cut(s) 1091
BshNI GGYRCC 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 144
BsiEI CGRYCG 2 cut(s) 241, 1051
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 4 cut(s) 417, 546, 976, 1058
BslI CCNNNNNNNGG 4 cut(s) 163, 194, 340, 542
BsmAI GTCTC 2 cut(s) 194, 909
BsmFI GGGAC 4 cut(s) 417, 546, 976, 1058
BsmI GAATGC 2 cut(s) 468, 702
BsnI GGCC 1 cut(s) 1091
Bso31I GGTCTC 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 835
Bsp1720I GCTNAGC 1 cut(s) 885
BspACI CCGC 3 cut(s) 71, 563, 572
BspANI GGCC 1 cut(s) 1091
BspCNI CTCAG 2 cut(s) 784, 877
BspDI ATCGAT 1 cut(s) 144
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 4 cut(s) 168, 367, 534, 678
BspMAI CTGCAG 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 676
BspTI CTTAAG 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 258, 630
BsrI ACTGG 3 cut(s) 133, 1046, 1075
BssECI CCNNGG 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 1086
Bst2UI CCWGG 2 cut(s) 681, 1002
Bst4CI ACNGT 7 cut(s) 58, 83, 239, 560, 881, 1049, 1149
Bst6I CTCTTC 1 cut(s) 333
BstAFI CTTAAG 1 cut(s) 812
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstBI TTCGAA 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 75, 946
BstDEI CTNAG 3 cut(s) 434, 792, 885
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 2 cut(s) 66, 844
BstFNI CGCG 1 cut(s) 572
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 2 cut(s) 228, 330
BstMAI GTCTC 2 cut(s) 194, 909
BstMCI CGRYCG 2 cut(s) 241, 1051
BstMWI GCNNNNNNNGC 1 cut(s) 1025
BstNI CCWGG 2 cut(s) 681, 1002
BstNSI RCATGY 2 cut(s) 77, 948
BstSCI CCNGG 3 cut(s) 221, 679, 1000
BstSFI CTRYAG 1 cut(s) 496
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 215, 218, 1079
BstV2I GAAGAC 1 cut(s) 989
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 1 cut(s) 1091
BsuTUI ATCGAT 1 cut(s) 144
BtsCI GGATG 2 cut(s) 66, 844
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 4 cut(s) 369, 381, 886, 1082
Cac8I GCNNGC 2 cut(s) 75, 946
CfoI GCGC 2 cut(s) 228, 330
Cfr13I GGNCC 2 cut(s) 167, 533
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 4 cut(s) 172, 287, 677, 1128
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 6 cut(s) 31, 74, 656, 851, 945, 990
CviQI GTAC 4 cut(s) 172, 287, 677, 1128
DdeI CTNAG 3 cut(s) 434, 792, 885
DraIII CACNNNGTG 1 cut(s) 992
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 1086
Eco147I AGGCCT 1 cut(s) 1091
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 167, 533
Eco57I CTGAAG 3 cut(s) 474, 615, 1083
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 2 cut(s) 679, 1000
EcoT14I CCWWGG 1 cut(s) 1086
ErhI CCWWGG 1 cut(s) 1086
FaeI CATG 6 cut(s) 34, 77, 659, 854, 948, 993
FaqI GGGAC 4 cut(s) 417, 546, 976, 1058
FatI CATG 6 cut(s) 30, 73, 655, 850, 944, 989
FauI CCCGC 2 cut(s) 78, 565
FauNDI CATATG 2 cut(s) 193, 601
Fnu4HI GCNGC 3 cut(s) 229, 232, 1068
FokI GGATG 2 cut(s) 53, 831
Fsp4HI GCNGC 3 cut(s) 229, 232, 1068
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 227, 329
GluI GCNGC 3 cut(s) 229, 232, 1068
GsuI CTGGAG 1 cut(s) 891
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 1 cut(s) 1091
HapII CCGG 1 cut(s) 222
HhaI GCGC 2 cut(s) 228, 330
Hin1II CATG 6 cut(s) 34, 77, 659, 854, 948, 993
Hin6I GCGC 2 cut(s) 226, 328
HinP1I GCGC 2 cut(s) 226, 328
HincII GTYRAC 2 cut(s) 759, 856
HindII GTYRAC 2 cut(s) 759, 856
HinfI GANTC 4 cut(s) 113, 266, 649, 832
HpaI GTTAAC 1 cut(s) 759
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 3 cut(s) 759, 856, 877
Hpy188I TCNGA 4 cut(s) 493, 736, 793, 831
Hpy188III TCNNGA 6 cut(s) 299, 307, 392, 613, 908, 939
Hpy8I GTNNAC 3 cut(s) 759, 856, 877
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 851
HpyCH4III ACNGT 7 cut(s) 58, 83, 239, 560, 881, 1049, 1149
HpyCH4IV ACGT 3 cut(s) 36, 312, 406
HpyCH4V TGCA 7 cut(s) 356, 374, 480, 498, 702, 1019, 1028
HpyF10VI GCNNNNNNNGC 1 cut(s) 1025
HpyF3I CTNAG 3 cut(s) 434, 792, 885
HpySE526I ACGT 3 cut(s) 36, 312, 406
Hsp92II CATG 6 cut(s) 34, 77, 659, 854, 948, 993
HspAI GCGC 2 cut(s) 226, 328
KpnI GGTACC 1 cut(s) 680
KspAI GTTAAC 1 cut(s) 759
LmnI GCTCC 3 cut(s) 54, 365, 1004
Lsp1109I GCAGC 3 cut(s) 215, 218, 1079
LweI GCATC 2 cut(s) 136, 671
MaeI CTAG 1 cut(s) 284
MaeII ACGT 3 cut(s) 36, 312, 406
MaeIII GTNAC 1 cut(s) 152
MboII GAAGA 5 cut(s) 172, 175, 350, 480, 989
MfeI CAATTG 2 cut(s) 693, 893
MluCI AATT 9 cut(s) 246, 349, 438, 588, 693, 893, 914, 970, 1104
MmeI TCCRAC 1 cut(s) 343
MseI TTAA 6 cut(s) 245, 758, 779, 813, 824, 1097
MslI CAYNNNNRTG 4 cut(s) 475, 527, 849, 1147
MspCI CTTAAG 1 cut(s) 812
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 3 cut(s) 223, 681, 1002
MunI CAATTG 2 cut(s) 693, 893
Mva1269I GAATGC 2 cut(s) 468, 702
MvaI CCWGG 2 cut(s) 681, 1002
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 1025
NciI CCSGG 1 cut(s) 223
NdeI CATATG 2 cut(s) 193, 601
NlaIII CATG 6 cut(s) 34, 77, 659, 854, 948, 993
NlaIV GGNNCC 4 cut(s) 168, 367, 534, 678
NspI RCATGY 2 cut(s) 77, 948
NspV TTCGAA 1 cut(s) 835
OliI CACNNNNGTG 1 cut(s) 1147
PaeI GCATGC 2 cut(s) 77, 948
PceI AGGCCT 1 cut(s) 1091
PctI GAATGC 2 cut(s) 468, 702
PfeI GAWTC 4 cut(s) 113, 266, 649, 832
PfoI TCCNGGA 1 cut(s) 1000
PkrI GCNGC 3 cut(s) 230, 233, 1069
PsiI TTATAA 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 679, 1000
PspGI CCWGG 2 cut(s) 679, 1000
PspN4I GGNNCC 4 cut(s) 168, 367, 534, 678
PspPI GGNCC 2 cut(s) 167, 533
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 500
RsaI GTAC 4 cut(s) 173, 288, 678, 1129
RsaNI GTAC 4 cut(s) 172, 287, 677, 1128
RseI CAYNNNNRTG 4 cut(s) 475, 527, 849, 1147
SaqAI TTAA 6 cut(s) 245, 758, 779, 813, 824, 1097
SatI GCNGC 3 cut(s) 229, 232, 1068
Sau96I GGNCC 2 cut(s) 167, 533
ScrFI CCNGG 3 cut(s) 223, 681, 1002
SfaNI GCATC 2 cut(s) 136, 671
SfcI CTRYAG 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 835
SinI GGWCC 2 cut(s) 167, 533
SmiMI CAYNNNNRTG 4 cut(s) 475, 527, 849, 1147
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SpeI ACTAGT 1 cut(s) 283
SphI GCATGC 2 cut(s) 77, 948
Sse9I AATT 9 cut(s) 246, 349, 438, 588, 693, 893, 914, 970, 1104
SseBI AGGCCT 1 cut(s) 1091
SsiI CCGC 3 cut(s) 71, 563, 572
SspMI CTAG 1 cut(s) 284
StuI AGGCCT 1 cut(s) 1091
StyD4I CCNGG 3 cut(s) 221, 679, 1000
StyI CCWWGG 1 cut(s) 1086
TaaI ACNGT 7 cut(s) 58, 83, 239, 560, 881, 1049, 1149
TaiI ACGT 3 cut(s) 39, 315, 409
TaqI TCGA 3 cut(s) 144, 665, 835
TaqII GACCGA 1 cut(s) 550
TasI AATT 9 cut(s) 246, 349, 438, 588, 693, 893, 914, 970, 1104
TfiI GAWTC 4 cut(s) 113, 266, 649, 832
Tru1I TTAA 6 cut(s) 245, 758, 779, 813, 824, 1097
Tru9I TTAA 6 cut(s) 245, 758, 779, 813, 824, 1097
TscAI CASTG 4 cut(s) 376, 388, 886, 1082
TseI GCWGC 3 cut(s) 228, 231, 1067
TspDTI ATGAA 4 cut(s) 47, 200, 333, 833
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 4 cut(s) 376, 388, 886, 1082
Vha464I CTTAAG 1 cut(s) 812
VpaK11BI GGWCC 2 cut(s) 167, 533
XagI CCTNNNNNAGG 1 cut(s) 338
XapI RAATTY 3 cut(s) 349, 438, 1104
XceI RCATGY 2 cut(s) 77, 948
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.