pycom10g23640

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001486
Physical Location & Seq
Reverse (-)
25714408 .. 25716762
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
ATGCTTGTGGGTAGATTCAGCCTAAGAAGCTACTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATTTTGATGACTCTCCTCCTCCTCCTCATGATGTCAGTTGTCACATTCATCCCTCAAGCTCTAGGTTGCTACAGCTCAATCATCAGCTTCGGCGATTCAATTGCCGATACGGGCAACTTGTACAACAGCTCTCCTAACAAATCTTTGGACTTTTTCTTCCCCCCATATGGAGAGACCTACTTTCACCGCCCGGTCGGACGCTGCTCCGACGGTCGTTTAATCATAGACTTCATTGCTGAATATATGGGACTACCACTAGTGCAACCTTACCTCGAAAGCCATAACCAAACTGTCCACAACATTGAAGGTGGTGTGAATTTTGCAGTGGTAGGATCCACTGCGCTGGACGCTGCTTTTCTTGAAGAAATGGGAGTTTATAATGACATTACAAACAACTCTCTAAGAATTCAACTGGACTGGTTCCAAGAAATGCTGTCATTTTTATGCAACACATCTTCAGACTGCAACAAACTGCTTTCGAGCTCTCTAGTTCTTATGGGAGAGATCGGAGGCAACGATTACAACAACGCATTGCTTGGAGGAAAAAGTATAGAAAAGGTTGAGAAATATGTTCCATTGGTGATAGAAGCAATAGCTTCAACAATCAATGAGTTGATTGCTTATGGCGCTTCAACACTTGTTGTACCCGGAAACTTTCCAATTGGCTGCATTCCTGCTTATCTAACAACATATGAAACTTCAGATGAGAGTGAATACGACCCTTTAACTGGCTGTCTAAACTGGCTGAACAAGTTTTCCGAGTATCACAATGAGCTGCTCCAGAGAGAACTTAGTCGGATTCGAAGCCTTCATCCTTATGTCAATATCATTTACGCAGATTACTACAATGCCATGCTGCAACTGTACCAGTCTCCAGACCAATTTGGATTTACTGGGGAAACTACCAAAGCATGCTGTGGAGGTGGGGGGCCGTACAATTACAACTCATCAGCACTATGTGGCAGTCCAGAGGCAAATGTACTTTGTGAAAACCCTGCACAGTTTATCAGCTGGGATGGTGTGCATTCAACTGAAGCAGCATACCGGTGGATAGCCAAGGGTTTATTAAACGGGAATTACACCGTTCCGCAAATTAGTACCTTATGTGTTTCACAACTGTGA

Protein Analysis

405

Amino Acids

45.28

Weight (kDa)

4.78

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 385 7.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
AciI CCGC 2 cut(s) 280, 1181
AclWI GGATC 2 cut(s) 420, 433
AcsI RAATTY 3 cut(s) 93, 409, 498
AcuI CTGAAG 3 cut(s) 534, 777, 1146
AdeI CACNNNGTG 1 cut(s) 1052
AfaI GTAC 6 cut(s) 215, 738, 959, 1028, 1074, 1192
AfiI CCNNNNNNNGG 2 cut(s) 260, 821
AgeI ACCGGT 1 cut(s) 1137
AgsI TTSAA 7 cut(s) 192, 398, 455, 503, 693, 726, 1122
AhlI ACTAGT 1 cut(s) 349
AleI CACNNNNGTG 1 cut(s) 1210
AloI GAACNNNNNNTCC 2 cut(s) 833, 865
AluBI AGCT 9 cut(s) 30, 152, 168, 180, 222, 576, 689, 868, 1104
AluI AGCT 9 cut(s) 30, 152, 168, 180, 222, 576, 689, 868, 1104
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 2 cut(s) 260, 969
AlwI GGATC 2 cut(s) 420, 433
AoxI GGCC 1 cut(s) 1022
ApeKI GCWGC 6 cut(s) 294, 443, 759, 868, 949, 1130
ApoI RAATTY 3 cut(s) 93, 409, 498
AsiGI ACCGGT 1 cut(s) 1137
AspLEI GCGC 2 cut(s) 436, 722
AspS9I GGNCC 1 cut(s) 1022
AsuC2I CCSGG 2 cut(s) 284, 741
AsuHPI GGTGA 2 cut(s) 269, 685
AsuII TTCGAA 1 cut(s) 895
BamHI GGATCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 578
Bbv12I GWGCWC 1 cut(s) 578
BbvI GCAGC 6 cut(s) 281, 430, 746, 855, 936, 1142
BccI CCATC 1 cut(s) 1103
BceAI ACGGC 1 cut(s) 1009
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BcnI CCSGG 2 cut(s) 284, 741
BcoDI GTCTC 2 cut(s) 260, 969
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 3 cut(s) 155, 350, 581
BfmI CTRYAG 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 723
BisI GCNGC 6 cut(s) 295, 444, 760, 869, 950, 1131
BlsI GCNGC 6 cut(s) 296, 445, 761, 870, 951, 1132
Bme1390I CCNGG 2 cut(s) 284, 741
BmgT120I GGNCC 1 cut(s) 1022
BmiI GGNNCC 3 cut(s) 427, 515, 1023
BmrFI CCNGG 2 cut(s) 284, 741
BmrI ACTGGG 1 cut(s) 996
BmuI ACTGGG 1 cut(s) 996
BpmI CTGGAG 2 cut(s) 857, 951
Bpu14I TTCGAA 1 cut(s) 895
BpuEI CTTGAG 2 cut(s) 55, 132
BpuMI CCSGG 2 cut(s) 284, 741
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 1149
BsaWI WCCGGW 1 cut(s) 1137
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 821
Bse118I RCCGGY 1 cut(s) 1137
Bse1I ACTGG 6 cut(s) 510, 515, 826, 839, 961, 991
Bse3DI GCAATG 2 cut(s) 324, 623
BseDI CCNNGG 1 cut(s) 1149
BseGI GGATG 3 cut(s) 141, 904, 1114
BseLI CCNNNNNNNGG 2 cut(s) 260, 821
BseMI GCAATG 2 cut(s) 324, 623
BseNI ACTGG 6 cut(s) 510, 515, 826, 839, 961, 991
BseRI GAGGAG 4 cut(s) 98, 101, 104, 107
BseXI GCAGC 6 cut(s) 281, 430, 746, 855, 936, 1142
BseYI CCCAGC 1 cut(s) 1104
BsgI GTGCAG 1 cut(s) 1074
Bsh1285I CGRYCG 2 cut(s) 288, 307
BshFI GGCC 1 cut(s) 1024
BshTI ACCGGT 1 cut(s) 1137
BsiEI CGRYCG 2 cut(s) 288, 307
BsiHKAI GWGCWC 1 cut(s) 578
BsiSI CCGG 3 cut(s) 284, 741, 1138
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 2 cut(s) 260, 821
BsmAI GTCTC 2 cut(s) 260, 969
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 2 cut(s) 762, 1117
BsnI GGCC 1 cut(s) 1024
Bso31I GGTCTC 1 cut(s) 260
Bsp119I TTCGAA 1 cut(s) 895
Bsp1286I GDGCHC 1 cut(s) 578
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 2 cut(s) 425, 597
BspACI CCGC 2 cut(s) 280, 1181
BspANI GGCC 1 cut(s) 1024
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 427, 515, 1023
BspPI GGATC 2 cut(s) 420, 433
BspT104I TTCGAA 1 cut(s) 895
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 324, 623
BsrFI RCCGGY 1 cut(s) 1137
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 6 cut(s) 510, 515, 826, 839, 961, 991
BssAI RCCGGY 1 cut(s) 1137
BssECI CCNNGG 1 cut(s) 1149
BssMI GATC 2 cut(s) 425, 597
BssT1I CCWWGG 1 cut(s) 1149
Bst4CI ACNGT 6 cut(s) 305, 385, 957, 1095, 1177, 1212
BstAUI TGTACA 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 895
BstC8I GCNNGC 1 cut(s) 1006
BstDEI CTNAG 3 cut(s) 23, 494, 884
BstF5I GGATG 3 cut(s) 141, 904, 1114
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 2 cut(s) 436, 722
BstKTI GATC 2 cut(s) 428, 600
BstMAI GTCTC 2 cut(s) 260, 969
BstMBI GATC 2 cut(s) 425, 597
BstMCI CGRYCG 2 cut(s) 288, 307
BstMWI GCNNNNNNNGC 3 cut(s) 27, 440, 719
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 2 cut(s) 282, 739
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 6 cut(s) 281, 430, 746, 855, 936, 1142
BstX2I RGATCY 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 425
BsuRI GGCC 1 cut(s) 1024
BtsCI GGATG 3 cut(s) 141, 904, 1114
BtsI GCAGTG 2 cut(s) 423, 429
BtsIMutI CAGTG 2 cut(s) 423, 429
Cac8I GCNNGC 1 cut(s) 1006
CciI TCATGA 1 cut(s) 120
CfoI GCGC 2 cut(s) 436, 722
Cfr10I RCCGGY 1 cut(s) 1137
Cfr13I GGNCC 1 cut(s) 1022
CseI GACGC 2 cut(s) 300, 449
Csp6I GTAC 6 cut(s) 214, 737, 958, 1027, 1073, 1191
CspAI ACCGGT 1 cut(s) 1137
CviAII CATG 3 cut(s) 121, 946, 1005
CviQI GTAC 6 cut(s) 214, 737, 958, 1027, 1073, 1191
DdeI CTNAG 3 cut(s) 23, 494, 884
DpnI GATC 2 cut(s) 427, 599
DpnII GATC 2 cut(s) 425, 597
DraIII CACNNNGTG 1 cut(s) 1052
Ecl136II GAGCTC 1 cut(s) 576
Eco130I CCWWGG 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 578
Eco31I GGTCTC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 576
Eco57I CTGAAG 3 cut(s) 534, 777, 1146
EcoICRI GAGCTC 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 1149
EcoT38I GRGCYC 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 1149
FaeI CATG 3 cut(s) 124, 949, 1008
FaqI GGGAC 1 cut(s) 354
FatI CATG 3 cut(s) 120, 945, 1004
FauNDI CATATG 2 cut(s) 259, 784
Fnu4HI GCNGC 6 cut(s) 295, 444, 760, 869, 950, 1131
FokI GGATG 3 cut(s) 128, 891, 1121
FriOI GRGCYC 1 cut(s) 578
Fsp4HI GCNGC 6 cut(s) 295, 444, 760, 869, 950, 1131
FspBI CTAG 3 cut(s) 155, 350, 581
GlaI GCGC 2 cut(s) 435, 721
GluI GCNGC 6 cut(s) 295, 444, 760, 869, 950, 1131
GsaI CCCAGC 1 cut(s) 1108
GsuI CTGGAG 2 cut(s) 857, 951
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 1 cut(s) 1024
HapII CCGG 3 cut(s) 284, 741, 1138
HgaI GACGC 2 cut(s) 300, 449
HhaI GCGC 2 cut(s) 436, 722
Hin1II CATG 3 cut(s) 124, 949, 1008
Hin6I GCGC 2 cut(s) 434, 720
HinP1I GCGC 2 cut(s) 434, 720
HinfI GANTC 4 cut(s) 15, 103, 188, 892
HpaII CCGG 3 cut(s) 284, 741, 1138
HphI GGTGA 2 cut(s) 269, 685
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 7 cut(s) 290, 301, 553, 602, 796, 853, 891
Hpy188III TCNNGA 5 cut(s) 121, 452, 874, 968, 1061
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 392, 911
HpyCH4III ACNGT 6 cut(s) 305, 385, 957, 1095, 1177, 1212
HpyCH4V TGCA 8 cut(s) 355, 416, 540, 558, 762, 952, 1091, 1117
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 440, 719
HpyF3I CTNAG 3 cut(s) 23, 494, 884
Hsp92II CATG 3 cut(s) 124, 949, 1008
HspAI GCGC 2 cut(s) 434, 720
Kzo9I GATC 2 cut(s) 425, 597
LmnI GCTCC 2 cut(s) 302, 876
Lsp1109I GCAGC 6 cut(s) 281, 430, 746, 855, 936, 1142
MaeI CTAG 3 cut(s) 155, 350, 581
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 427, 599
MboI GATC 2 cut(s) 425, 597
MboII GAAGA 3 cut(s) 241, 467, 540
MfeI CAATTG 2 cut(s) 192, 753
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 9 cut(s) 93, 192, 409, 498, 753, 974, 1030, 1168, 1185
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 3 cut(s) 268, 324, 869
MseI TTAA 3 cut(s) 311, 818, 1160
MslI CAYNNNNRTG 4 cut(s) 535, 909, 1138, 1210
MspA1I CMGCKG 1 cut(s) 1104
MspI CCGG 3 cut(s) 284, 741, 1138
MspR9I CCNGG 2 cut(s) 284, 741
MunI CAATTG 2 cut(s) 192, 753
Mva1269I GAATGC 2 cut(s) 762, 1117
MwoI GCNNNNNNNGC 3 cut(s) 27, 440, 719
NciI CCSGG 2 cut(s) 284, 741
NdeI CATATG 2 cut(s) 259, 784
NdeII GATC 2 cut(s) 425, 597
NlaIII CATG 3 cut(s) 124, 949, 1008
NlaIV GGNNCC 3 cut(s) 427, 515, 1023
NmuCI GTSAC 1 cut(s) 133
NspI RCATGY 1 cut(s) 1008
NspV TTCGAA 1 cut(s) 895
OliI CACNNNNGTG 1 cut(s) 1210
PaeI GCATGC 1 cut(s) 1008
PagI TCATGA 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PctI GAATGC 2 cut(s) 762, 1117
PfeI GAWTC 3 cut(s) 15, 188, 892
PinAI ACCGGT 1 cut(s) 1137
PkrI GCNGC 6 cut(s) 296, 445, 761, 870, 951, 1132
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PsiI TTATAA 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 1104
PspN4I GGNNCC 3 cut(s) 427, 515, 1023
PspPI GGNCC 1 cut(s) 1022
PsuI RGATCY 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 1104
RsaI GTAC 6 cut(s) 215, 738, 959, 1028, 1074, 1192
RsaNI GTAC 6 cut(s) 214, 737, 958, 1027, 1073, 1191
RseI CAYNNNNRTG 4 cut(s) 535, 909, 1138, 1210
SacI GAGCTC 1 cut(s) 578
SaqAI TTAA 3 cut(s) 311, 818, 1160
SatI GCNGC 6 cut(s) 295, 444, 760, 869, 950, 1131
Sau3AI GATC 2 cut(s) 425, 597
Sau96I GGNCC 1 cut(s) 1022
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 2 cut(s) 284, 741
SduI GDGCHC 1 cut(s) 578
SfcI CTRYAG 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 895
SmiMI CAYNNNNRTG 4 cut(s) 535, 909, 1138, 1210
SmlI CTYRAG 2 cut(s) 34, 147
SmoI CTYRAG 2 cut(s) 34, 147
SpeI ACTAGT 1 cut(s) 349
SphI GCATGC 1 cut(s) 1008
Sse9I AATT 9 cut(s) 93, 192, 409, 498, 753, 974, 1030, 1168, 1185
SsiI CCGC 2 cut(s) 280, 1181
SspMI CTAG 3 cut(s) 155, 350, 581
SstI GAGCTC 1 cut(s) 578
StyD4I CCNGG 2 cut(s) 282, 739
StyI CCWWGG 1 cut(s) 1149
TaaI ACNGT 6 cut(s) 305, 385, 957, 1095, 1177, 1212
TaqI TCGA 3 cut(s) 366, 572, 895
TasI AATT 9 cut(s) 93, 192, 409, 498, 753, 974, 1030, 1168, 1185
TatI WGTACW 2 cut(s) 213, 1072
TfiI GAWTC 3 cut(s) 15, 188, 892
Tru1I TTAA 3 cut(s) 311, 818, 1160
Tru9I TTAA 3 cut(s) 311, 818, 1160
TscAI CASTG 2 cut(s) 423, 436
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 6 cut(s) 294, 443, 759, 868, 949, 1130
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 4 cut(s) 130, 313, 801, 893
TspRI CASTG 2 cut(s) 423, 436
XapI RAATTY 3 cut(s) 93, 409, 498
XceI RCATGY 1 cut(s) 1008
XspI CTAG 3 cut(s) 155, 350, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.