Belongs to the glycosyltransferase 2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1235 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 589 |
| AatII | GACGTC | 1 cut(s) | 1017 |
| AccB1I | GGYRCC | 2 cut(s) | 413, 548 |
| AccI | GTMKAC | 1 cut(s) | 1008 |
| AciI | CCGC | 1 cut(s) | 319 |
| AclWI | GGATC | 2 cut(s) | 512, 1148 |
| AcsI | RAATTY | 2 cut(s) | 98, 689 |
| AcyI | GRCGYC | 1 cut(s) | 1014 |
| AfaI | GTAC | 1 cut(s) | 205 |
| AgsI | TTSAA | 5 cut(s) | 86, 154, 652, 1105, 1223 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1151, 1183 |
| Alw21I | GWGCWC | 1 cut(s) | 861 |
| Alw26I | GTCTC | 1 cut(s) | 793 |
| AlwI | GGATC | 2 cut(s) | 512, 1148 |
| AoxI | GGCC | 3 cut(s) | 188, 527, 643 |
| ApoI | RAATTY | 2 cut(s) | 98, 689 |
| AspS9I | GGNCC | 5 cut(s) | 188, 290, 574, 591, 644 |
| AsuC2I | CCSGG | 1 cut(s) | 259 |
| AvaII | GGWCC | 3 cut(s) | 290, 574, 591 |
| AxyI | CCTNAGG | 1 cut(s) | 783 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1260, 1293 |
| BanI | GGYRCC | 2 cut(s) | 413, 548 |
| Bbv12I | GWGCWC | 1 cut(s) | 861 |
| BccI | CCATC | 4 cut(s) | 170, 301, 404, 582 |
| BcnI | CCSGG | 1 cut(s) | 259 |
| BcoDI | GTCTC | 1 cut(s) | 793 |
| BfaI | CTAG | 6 cut(s) | 50, 74, 389, 1094, 1134, 1274 |
| Bme1390I | CCNGG | 1 cut(s) | 259 |
| Bme18I | GGWCC | 3 cut(s) | 290, 574, 591 |
| BmgT120I | GGNCC | 5 cut(s) | 188, 290, 574, 591, 644 |
| BmiI | GGNNCC | 2 cut(s) | 415, 550 |
| BmrFI | CCNGG | 1 cut(s) | 259 |
| BmsI | GCATC | 4 cut(s) | 253, 401, 940, 1121 |
| BpuEI | CTTGAG | 1 cut(s) | 1010 |
| BpuMI | CCSGG | 1 cut(s) | 259 |
| BsaHI | GRCGYC | 1 cut(s) | 1014 |
| BsaI | GGTCTC | 1 cut(s) | 793 |
| BsaJI | CCNNGG | 2 cut(s) | 258, 577 |
| Bse1I | ACTGG | 3 cut(s) | 205, 211, 984 |
| Bse21I | CCTNAGG | 1 cut(s) | 783 |
| Bse3DI | GCAATG | 4 cut(s) | 4, 42, 273, 487 |
| BseDI | CCNNGG | 2 cut(s) | 258, 577 |
| BseGI | GGATG | 7 cut(s) | 268, 293, 568, 830, 915, 926, 955 |
| BseMI | GCAATG | 4 cut(s) | 4, 42, 273, 487 |
| BseMII | CTCAG | 1 cut(s) | 797 |
| BseNI | ACTGG | 3 cut(s) | 205, 211, 984 |
| BshFI | GGCC | 3 cut(s) | 190, 529, 645 |
| BshNI | GGYRCC | 2 cut(s) | 413, 548 |
| BsiHKAI | GWGCWC | 1 cut(s) | 861 |
| BsiSI | CCGG | 2 cut(s) | 258, 597 |
| BslFI | GGGAC | 1 cut(s) | 595 |
| BsmAI | GTCTC | 1 cut(s) | 793 |
| BsmFI | GGGAC | 1 cut(s) | 595 |
| BsmI | GAATGC | 3 cut(s) | 768, 1233, 1254 |
| BsnI | GGCC | 3 cut(s) | 190, 529, 645 |
| Bso31I | GGTCTC | 1 cut(s) | 793 |
| Bsp1286I | GDGCHC | 1 cut(s) | 861 |
| Bsp143I | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| BspACI | CCGC | 1 cut(s) | 319 |
| BspANI | GGCC | 3 cut(s) | 190, 529, 645 |
| BspCNI | CTCAG | 1 cut(s) | 796 |
| BspHI | TCATGA | 1 cut(s) | 957 |
| BspLI | GGNNCC | 2 cut(s) | 415, 550 |
| BspPI | GGATC | 2 cut(s) | 512, 1148 |
| BspT107I | GGYRCC | 2 cut(s) | 413, 548 |
| BspTNI | GGTCTC | 1 cut(s) | 793 |
| BsrDI | GCAATG | 4 cut(s) | 4, 42, 273, 487 |
| BsrI | ACTGG | 3 cut(s) | 205, 211, 984 |
| BssECI | CCNNGG | 2 cut(s) | 258, 577 |
| BssMI | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| BssNI | GRCGYC | 1 cut(s) | 1014 |
| BssT1I | CCWWGG | 1 cut(s) | 577 |
| Bst4CI | ACNGT | 2 cut(s) | 134, 449 |
| BstACI | GRCGYC | 1 cut(s) | 1014 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 398, 692 |
| BstC8I | GCNNGC | 1 cut(s) | 33 |
| BstDEI | CTNAG | 3 cut(s) | 275, 783, 1285 |
| BstF5I | GGATG | 7 cut(s) | 268, 293, 568, 830, 915, 926, 955 |
| BstKTI | GATC | 5 cut(s) | 146, 340, 520, 568, 1143 |
| BstMAI | GTCTC | 1 cut(s) | 793 |
| BstMBI | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 32, 187, 263, 398, 692 |
| BstNSI | RCATGY | 1 cut(s) | 679 |
| BstSCI | CCNGG | 1 cut(s) | 257 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 559, 938 |
| Bsu36I | CCTNAGG | 1 cut(s) | 783 |
| BsuRI | GGCC | 3 cut(s) | 190, 529, 645 |
| BtsCI | GGATG | 7 cut(s) | 268, 293, 568, 830, 915, 926, 955 |
| BtsI | GCAGTG | 1 cut(s) | 550 |
| BtsIMutI | CAGTG | 3 cut(s) | 459, 513, 550 |
| Cac8I | GCNNGC | 1 cut(s) | 33 |
| CciI | TCATGA | 1 cut(s) | 957 |
| Cfr13I | GGNCC | 5 cut(s) | 188, 290, 574, 591, 644 |
| Csp6I | GTAC | 1 cut(s) | 204 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 519, 554 |
| CviAII | CATG | 5 cut(s) | 11, 676, 958, 1108, 1327 |
| CviQI | GTAC | 1 cut(s) | 204 |
| DdeI | CTNAG | 3 cut(s) | 275, 783, 1285 |
| DpnI | GATC | 5 cut(s) | 145, 339, 519, 567, 1142 |
| DpnII | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 589 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 589 |
| EciI | GGCGGA | 1 cut(s) | 308 |
| Eco130I | CCWWGG | 1 cut(s) | 577 |
| Eco31I | GGTCTC | 1 cut(s) | 793 |
| Eco32I | GATATC | 2 cut(s) | 63, 124 |
| Eco47I | GGWCC | 3 cut(s) | 290, 574, 591 |
| Eco81I | CCTNAGG | 1 cut(s) | 783 |
| EcoRV | GATATC | 2 cut(s) | 63, 124 |
| EcoT14I | CCWWGG | 1 cut(s) | 577 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 577 |
| FaeI | CATG | 5 cut(s) | 14, 679, 961, 1111, 1330 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1215, 1247 |
| FaqI | GGGAC | 1 cut(s) | 595 |
| FatI | CATG | 5 cut(s) | 10, 675, 957, 1107, 1326 |
| FblI | GTMKAC | 1 cut(s) | 1008 |
| FokI | GGATG | 7 cut(s) | 275, 280, 575, 817, 913, 922, 962 |
| FspBI | CTAG | 6 cut(s) | 50, 74, 389, 1094, 1134, 1274 |
| HaeIII | GGCC | 3 cut(s) | 190, 529, 645 |
| HapII | CCGG | 2 cut(s) | 258, 597 |
| Hin1I | GRCGYC | 1 cut(s) | 1014 |
| Hin1II | CATG | 5 cut(s) | 14, 679, 961, 1111, 1330 |
| HincII | GTYRAC | 2 cut(s) | 873, 1009 |
| HindII | GTYRAC | 2 cut(s) | 873, 1009 |
| HpaII | CCGG | 2 cut(s) | 258, 597 |
| Hpy166II | GTNNAC | 2 cut(s) | 873, 1009 |
| Hpy188III | TCNNGA | 6 cut(s) | 196, 220, 619, 958, 1065, 1102 |
| Hpy8I | GTNNAC | 2 cut(s) | 873, 1009 |
| Hpy99I | CGWCG | 2 cut(s) | 1013, 1016 |
| HpyAV | CCTTC | 3 cut(s) | 148, 1198, 1217 |
| HpyCH4III | ACNGT | 2 cut(s) | 134, 449 |
| HpyCH4IV | ACGT | 1 cut(s) | 1014 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 105, 266, 365, 487, 492, 686 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 32, 187, 263, 398, 692 |
| HpyF3I | CTNAG | 3 cut(s) | 275, 783, 1285 |
| HpySE526I | ACGT | 1 cut(s) | 1014 |
| Hsp92I | GRCGYC | 1 cut(s) | 1014 |
| Hsp92II | CATG | 5 cut(s) | 14, 679, 961, 1111, 1330 |
| Kzo9I | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| LweI | GCATC | 4 cut(s) | 253, 401, 940, 1121 |
| MaeI | CTAG | 6 cut(s) | 50, 74, 389, 1094, 1134, 1274 |
| MaeII | ACGT | 1 cut(s) | 1014 |
| MaeIII | GTNAC | 5 cut(s) | 113, 196, 922, 1031, 1267 |
| MalI | GATC | 5 cut(s) | 145, 339, 519, 567, 1142 |
| MboI | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| MboII | GAAGA | 4 cut(s) | 419, 473, 659, 973 |
| MhlI | GDGCHC | 1 cut(s) | 861 |
| MluCI | AATT | 4 cut(s) | 86, 98, 556, 689 |
| MmeI | TCCRAC | 4 cut(s) | 479, 1019, 1042, 1137 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 1 cut(s) | 627 |
| MslI | CAYNNNNRTG | 3 cut(s) | 9, 936, 1202 |
| MspI | CCGG | 2 cut(s) | 258, 597 |
| MspR9I | CCNGG | 1 cut(s) | 259 |
| Mva1269I | GAATGC | 3 cut(s) | 768, 1233, 1254 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 32, 187, 263, 398, 692 |
| NciI | CCSGG | 1 cut(s) | 259 |
| NdeII | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| NlaIII | CATG | 5 cut(s) | 14, 679, 961, 1111, 1330 |
| NlaIV | GGNNCC | 2 cut(s) | 415, 550 |
| NmeAIII | GCCGAG | 1 cut(s) | 7 |
| NmuCI | GTSAC | 2 cut(s) | 196, 922 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 679 |
| PagI | TCATGA | 1 cut(s) | 957 |
| PctI | GAATGC | 3 cut(s) | 768, 1233, 1254 |
| PflFI | GACNNNGTC | 1 cut(s) | 1013 |
| PsiI | TTATAA | 1 cut(s) | 1235 |
| PspN4I | GGNNCC | 2 cut(s) | 415, 550 |
| PspPI | GGNCC | 5 cut(s) | 188, 290, 574, 591, 644 |
| PsyI | GACNNNGTC | 1 cut(s) | 1013 |
| RsaI | GTAC | 1 cut(s) | 205 |
| RsaNI | GTAC | 1 cut(s) | 204 |
| RseI | CAYNNNNRTG | 3 cut(s) | 9, 936, 1202 |
| SalI | GTCGAC | 1 cut(s) | 1007 |
| SaqAI | TTAA | 1 cut(s) | 627 |
| Sau3AI | GATC | 5 cut(s) | 143, 337, 517, 565, 1140 |
| Sau96I | GGNCC | 5 cut(s) | 188, 290, 574, 591, 644 |
| ScrFI | CCNGG | 1 cut(s) | 259 |
| SduI | GDGCHC | 1 cut(s) | 861 |
| SetI | ASST | 8 cut(s) | 51, 159, 172, 435, 499, 789, 858, 1017 |
| SfaNI | GCATC | 4 cut(s) | 253, 401, 940, 1121 |
| SinI | GGWCC | 3 cut(s) | 290, 574, 591 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 9, 936, 1202 |
| SmlI | CTYRAG | 1 cut(s) | 1025 |
| SmoI | CTYRAG | 1 cut(s) | 1025 |
| Sse9I | AATT | 4 cut(s) | 86, 98, 556, 689 |
| SsiI | CCGC | 1 cut(s) | 319 |
| SspMI | CTAG | 6 cut(s) | 50, 74, 389, 1094, 1134, 1274 |
| StyD4I | CCNGG | 1 cut(s) | 257 |
| StyI | CCWWGG | 1 cut(s) | 577 |
| TaaI | ACNGT | 2 cut(s) | 134, 449 |
| TaiI | ACGT | 1 cut(s) | 1017 |
| TaqI | TCGA | 3 cut(s) | 620, 880, 1008 |
| TasI | AATT | 4 cut(s) | 86, 98, 556, 689 |
| Tru1I | TTAA | 1 cut(s) | 627 |
| Tru9I | TTAA | 1 cut(s) | 627 |
| TscAI | CASTG | 3 cut(s) | 459, 513, 550 |
| TseFI | GTSAC | 2 cut(s) | 196, 922 |
| Tsp45I | GTSAC | 2 cut(s) | 196, 922 |
| TspRI | CASTG | 3 cut(s) | 459, 513, 550 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1013 |
| VpaK11BI | GGWCC | 3 cut(s) | 290, 574, 591 |
| XapI | RAATTY | 2 cut(s) | 98, 689 |
| XceI | RCATGY | 1 cut(s) | 679 |
| XmiI | GTMKAC | 1 cut(s) | 1008 |
| XspI | CTAG | 6 cut(s) | 50, 74, 389, 1094, 1134, 1274 |
| ZraI | GACGTC | 1 cut(s) | 1015 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |