Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000838

Physical Location & Seq

Genomic Coordinates Forward (+)

5379260 .. 5382670

Gene Structure 3411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G002750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGGATCAGAGTTCTAATGCCCCCATGCATCTCTGCCATGTCAACAAGCTCACAATCTTCATTAATCGAACGTATACAGTCATATACTCCGTCGCTTTATCATTCTTGCTTTACTATAGAGCCTCTTTCTTCTTCCATGACACCAAAACACCCATCTTACCATCTCTTATTGTCTTTGTGGCCGAGCTCCTCTCTTCTTTCGAGTGGCTTCTAACCCAGGCATATCGGTGGAGGCCCGTCTCTCGAACTGTGTTCCCGGAGAGATTGCCGGAAGATGACAAACTTCCTGCAGTCGACGTGTTCATTTGCACCGCTGATCCGGAGAAGGAGCCACCCGTGGAGGTGATGAACACTGTAATATCAGCCATGGCACTCGACTATCCACCCAAGAAGCTTCACGTGTATTTGTCAGATGATGGCGGTGCTGATGTGACTCTGAATGGCATGCGAGAGGCTTGGAAGTTTGCAAAGCGGTGGATTCCATTTTGTAAGAGGTATAAGATCAAGTGCAGGTCTCCGAAAGCCTATTTCTCAGCAGCAGAGGATGAGAATGAAGATGGTGATTTGGGGGGTACTGATTTCATACAAGAGAGACAAATTGTTCAGGAAAAATATGAGGTGTTTAAGGAAAGAGTGACAGAATACGCAAGGCATGGTGACACTGGGAGCAGTAAGCGTCGAGATCATTCTGCAGTGATTGAGGTGATACAAGATAGTTCTAGTAATCATGCATTTGAAGAAAATGAGGCAAAAAACATGCCTCTCCTTGTTTATGTCTCTCGCGAGAAAAGACCTTCTCATGCTCATCATTTCAAGGCTGGAGCTCTCAATGTTCTGCTTCGAGTATCCGGTGTGATCAGTAATTCTCCCTTCATATTAGGGCTTGATTGTGACATGTATTGCAATGATCCAAGTTCAGCTCGGCAAGCAATGTGTTTCCACCTTGATCCCCAGCTCTCGCCTACGCTAGCACTTGTGCAATTCCCTCAGAAATTCCACAACATCAGTAAGAATGACATCTACGACAGTCAGTTGAGAGAGACGTTTTCGGTGCTATGGCAAGGTTTTGATGGACTTCAAGGCCCATGCGTGTCCGGTACTGGCTATTACATCAAGAGGTTATCCTTGTATGGAAAATCCCTAAAGGAGGATCATAAGGATCTTATGGAACTTAGAACATTTGTTGGTCCATCAAGTGAGTTCATAAAATCCCTGCTTGGAAACTACAATCCTAATATGAAAATGGCACAGCTAGCTGAGACCCAAGAACTAGCCTCCTGTACCTATGAAGATGGCACCAAATGGGGTGAAGAGGCTGGTTTCTTATACGCGTCTGTAGCGGAAGATTATTTCACAGGGTTCGCTAGCTTGCATTGCAAGGGTTGGAATTCAGTGTATTGCAATCCGCCAAAGCCTCAGTTCTTGGGCAGTGGCACTACTAATTTGAATGACTTTTTGGTCCAAGGGACAAGATGGAGTTCTGGGTTGGTTGATGTTGCCATCTCCAAATACTGCCCTCTTATATATGGTCTTACTAAAATGTCTTTCCTTGAAAGTATGTGCTATGGATGGCTAGCAATTTTCCCTATTTGTCGGTTCTTCTCACTCTGGTGCCTTGCAACTATACCTCAGCTTTGCCTAGTCAACGGCATTTCCCTGTACCCTGAGGTCTCAAATTCGTATTTTTGGATATTTTCTTTTGTATTCCTCTCGTCTGTTTTGAGACATTTATACGAAGTCCTCACAACGGAAGCATCATTACAACATTGGATATACGAACAAAGAATATGGATGATGAAAGCAGTTACATCTCAATTATATGGTAGTCTTGATGCTTTTATGAAGAAATTTGGTTTGAGGGAAGCAAGCTTCTTCCCAACAAATAAAGTTGATGACGTTGAGCAACTCAAGCGGTACAACGCGGGGGTATTTGATTTTCAAACCTCAGCACTTTTTCTTGTTCCATTGGCTTCACTGGTTATCTTGAACATGGCCTCCCTTTGTGTGGGCATTGCCAGAGTGATCTTTCTGGGGGAATTGGAGAAGTTGTTTATACAAGTCTTCGTACCATTTTATGTGGTGGTGGTCAATTACCCTATTATAGAAGGAATGCTAATAAGGAAAGACAATGGTCGCATTCCACCATCTGTCACCCTATTGTCTGCTATAGCTTCAATCATTTTCTGCATTTTGGGTTCAATTATCTTCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009832 GO:0009833 GO:0009987 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0022402 GO:0030243 GO:0030244 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0051273 GO:0051274 GO:0051301 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901576 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt

Protein Analysis

737

Amino Acids

83.76

Weight (kDa)

6.18

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cellulose_synt	PF03552	98 - 386	7.8e-82	Cellulose synthase
Cellulose_synt	PF03552	390 - 731	2.6e-54	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000838)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g02981 FvH4_4g02990
malus_domestica	MD02G1095700.v1.1 MD13G1209200.v1.1 MD15G1415100.v1.1 MD15G1415200.v1.1 MD16G1211700.v1.1 MD16G1211900.v1.1 MD16G1212000.v1.1
prunus_persica	Prupe.1G033700_v2.0.a1 Prupe.1G033700_v2.0.a1 Prupe.1G033900_v2.0.a1 Prupe.1G033900_v2.0.a1 Prupe.4G193000_v2.0.a1 Prupe.4G193000_v2.0.a1 Prupe.7G089900_v2.0.a1
pyrus_communis	pycom12g05820 pycom12g05850 pycom13g18120 pycom13g18130 pycom16g17740
rosa_chinensis	RchiOBHm_Chr4g0391141 RchiOBHm_Chr4g0391161 RchiOBHm_Chr4g0391171 RchiOBHm_Chr7g0211861
rosa_laevigata	RLG00000002959 RLG00000003213 RLG00000009880 RLG00000009881 RLG00000009882 RLG00000009884 RLG00000009885
rosa_multiflora	Rmu_co8060516.1_g000001 Rmu_sc0002852.1_g000004 Rmu_sc0009005.1_g000004 Rmu_sc0009836.1_g000005 Rmu_sc0020388.1_g000001 Rmu_sc0039496.1_g000001
rosa_roxburghii	Rroxscaffold_3G00247330 Rroxscaffold_5G00336930 Rroxscaffold_5G00336950 Rroxscaffold_5G00336960 Rroxscaffold_5G00344820 Rroxscaffold_5G00344830 Rroxscaffold_5G00344850
rosa_rugosa	Rorug03G0334500 Rorug03G0334900
rosa_samantha	Rh4AG034900 Rh4AG035200 Rh4AG035400 Rh4BG030100 Rh4BG030200 Rh4BG030300 Rh4BG030500 Rh4BG030600 Rh4CG038700 Rh4CG038800 Rh4DG032100 Rh4DG032800 Rh4DG032900 Rh7AG262300 Rh7BG255800 Rh7DG268800
rosa_wichuraiana	Rw4G002720 Rw4G002750 Rw4G002760 Rw4G002770 Rw7G022360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1457
Acc36I	ACCTGC	1 cut(s)	501
AccB1I	GGYRCC	2 cut(s)	1295, 1611
AccI	GTMKAC	2 cut(s)	74, 294
AccII	CGCG	3 cut(s)	783, 1331, 1922
AccIII	TCCGGA	1 cut(s)	319
AciI	CCGC	7 cut(s)	312, 420, 472, 1340, 1406, 1912, 1922
AclWI	GGATC	6 cut(s)	12, 311, 902, 941, 1158, 1167
AcoI	YGGCCR	1 cut(s)	180
AcsI	RAATTY	4 cut(s)	992, 1387, 1675, 1847
AcvI	CACGTG	1 cut(s)	400
AfaI	GTAC	6 cut(s)	574, 1099, 1282, 1661, 1916, 2067
AfiI	CCNNNNNNNGG	3 cut(s)	1147, 1747, 2005
AflIII	ACRYGT	4 cut(s)	297, 399, 894, 1329
AgsI	TTSAA	9 cut(s)	737, 814, 1079, 1447, 1553, 1940, 1987, 2175, 2199
AjiI	CACGTC	1 cut(s)	298
AjnI	CCWGG	1 cut(s)	216
AjuI	GAANNNNNNNTTGG	2 cut(s)	1439, 1471
AleI	CACNNNNGTG	1 cut(s)	1609
AloI	GAACNNNNNNTCC	2 cut(s)	1979, 2011
Alw21I	GWGCWC	2 cut(s)	189, 826
Alw26I	GTCTC	8 cut(s)	244, 519, 586, 781, 1034, 1253, 1675, 1717
AlwI	GGATC	6 cut(s)	12, 311, 902, 941, 1158, 1167
Aor13HI	TCCGGA	1 cut(s)	319
AoxI	GGCC	4 cut(s)	180, 233, 1081, 1992
ApeKI	GCWGC	1 cut(s)	536
ApoI	RAATTY	4 cut(s)	992, 1387, 1675, 1847
AseI	ATTAAT	1 cut(s)	63
AspS9I	GGNCC	4 cut(s)	234, 1082, 1187, 1459
AsuC2I	CCSGG	1 cut(s)	257
AsuHPI	GGTGA	6 cut(s)	355, 572, 668, 715, 1319, 2143
AsuNHI	GCTAGC	4 cut(s)	967, 1252, 1364, 1573
AvaII	GGWCC	2 cut(s)	1187, 1459
AxyI	CCTNAGG	1 cut(s)	1665
BanI	GGYRCC	2 cut(s)	1295, 1611
BanII	GRGCYC	2 cut(s)	189, 826
BarI	GAAGNNNNNNTAC	2 cut(s)	1743, 1775
BbrPI	CACGTG	1 cut(s)	400
BbsI	GAAGAC	1 cut(s)	2053
Bbv12I	GWGCWC	2 cut(s)	189, 826
BbvCI	CCTCAGC	2 cut(s)	1629, 1945
BbvI	GCAGC	1 cut(s)	548
BceAI	ACGGC	1 cut(s)	1663
BciT130I	CCWGG	1 cut(s)	218
BciVI	GTATCC	1 cut(s)	856
BclI	TGATCA	1 cut(s)	855
BcnI	CCSGG	1 cut(s)	257
BcoDI	GTCTC	8 cut(s)	244, 519, 586, 781, 1034, 1253, 1675, 1717
BfaI	CTAG	7 cut(s)	720, 968, 1253, 1271, 1365, 1574, 1640
BfmI	CTRYAG	5 cut(s)	115, 288, 690, 1335, 2166
BfuAI	ACCTGC	1 cut(s)	501
BfuI	GTATCC	1 cut(s)	856
BisI	GCNGC	1 cut(s)	537
BlsI	GCNGC	1 cut(s)	538
Bme1390I	CCNGG	2 cut(s)	218, 257
Bme18I	GGWCC	2 cut(s)	1187, 1459
BmgBI	CACGTC	1 cut(s)	298
BmgT120I	GGNCC	4 cut(s)	234, 1082, 1187, 1459
BmiI	GGNNCC	3 cut(s)	330, 1297, 1613
BmrFI	CCNGG	2 cut(s)	218, 257
BmrI	ACTGGG	1 cut(s)	672
BmsI	GCATC	3 cut(s)	37, 1763, 1822
BmtI	GCTAGC	4 cut(s)	971, 1256, 1368, 1577
BmuI	ACTGGG	1 cut(s)	672
BoxI	GACNNNNGTC	1 cut(s)	2130
BpiI	GAAGAC	1 cut(s)	2053
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	1 cut(s)	840
Bpu10I	CCTNAGC	2 cut(s)	1629, 1945
BpuEI	CTTGAG	1 cut(s)	1892
BpuMI	CCSGG	1 cut(s)	257
BsaAI	YACGTR	1 cut(s)	400
BsaI	GGTCTC	3 cut(s)	519, 1253, 1675
BsaJI	CCNNGG	4 cut(s)	216, 336, 366, 1462
BsaWI	WCCGGW	3 cut(s)	319, 848, 1094
BsaXI	ACNNNNNCTCC	4 cut(s)	1979, 2009, 2033, 2063
Bsc4I	CCNNNNNNNGG	3 cut(s)	1147, 1747, 2005
Bse1I	ACTGG	3 cut(s)	667, 1105, 1980
Bse21I	CCTNAGG	1 cut(s)	1665
Bse3DI	GCAATG	4 cut(s)	910, 936, 1372, 2010
BseAI	TCCGGA	1 cut(s)	319
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	4 cut(s)	216, 336, 366, 1462
BseGI	GGATG	3 cut(s)	550, 1574, 1797
BseLI	CCNNNNNNNGG	3 cut(s)	1147, 1747, 2005
BseMI	GCAATG	4 cut(s)	910, 936, 1372, 2010
BseMII	CTCAG	7 cut(s)	546, 1001, 1248, 1430, 1643, 1656, 1959
BseNI	ACTGG	3 cut(s)	667, 1105, 1980
BseRI	GAGGAG	1 cut(s)	179
BseXI	GCAGC	1 cut(s)	548
BseYI	CCCAGC	1 cut(s)	951
BsgI	GTGCAG	1 cut(s)	529
Bsh1236I	CGCG	3 cut(s)	783, 1331, 1922
BshFI	GGCC	4 cut(s)	182, 235, 1083, 1994
BshNI	GGYRCC	2 cut(s)	1295, 1611
BsiHKAI	GWGCWC	2 cut(s)	189, 826
BsiSI	CCGG	5 cut(s)	257, 269, 320, 849, 1095
BslFI	GGGAC	1 cut(s)	1480
BslI	CCNNNNNNNGG	3 cut(s)	1147, 1747, 2005
BsmAI	GTCTC	8 cut(s)	244, 519, 586, 781, 1034, 1253, 1675, 1717
BsmBI	CGTCTC	2 cut(s)	244, 1034
BsmFI	GGGAC	1 cut(s)	1480
BsmI	GAATGC	2 cut(s)	2115, 2136
BsnI	GGCC	4 cut(s)	182, 235, 1083, 1994
Bso31I	GGTCTC	3 cut(s)	519, 1253, 1675
Bsp1286I	GDGCHC	2 cut(s)	189, 826
Bsp13I	TCCGGA	1 cut(s)	319
Bsp19I	CCATGG	1 cut(s)	366
Bsp68I	TCGCGA	1 cut(s)	783
BspACI	CCGC	7 cut(s)	312, 420, 472, 1340, 1406, 1912, 1922
BspANI	GGCC	4 cut(s)	182, 235, 1083, 1994
BspCNI	CTCAG	7 cut(s)	545, 1000, 1249, 1429, 1642, 1657, 1958
BspEI	TCCGGA	1 cut(s)	319
BspFNI	CGCG	3 cut(s)	783, 1331, 1922
BspLI	GGNNCC	3 cut(s)	330, 1297, 1613
BspMAI	CTGCAG	2 cut(s)	292, 694
BspMI	ACCTGC	1 cut(s)	501
BspOI	GCTAGC	4 cut(s)	971, 1256, 1368, 1577
BspPI	GGATC	6 cut(s)	12, 311, 902, 941, 1158, 1167
BspT107I	GGYRCC	2 cut(s)	1295, 1611
BspTNI	GGTCTC	3 cut(s)	519, 1253, 1675
BsrDI	GCAATG	4 cut(s)	910, 936, 1372, 2010
BsrI	ACTGG	3 cut(s)	667, 1105, 1980
BssECI	CCNNGG	4 cut(s)	216, 336, 366, 1462
BssNAI	GTATAC	1 cut(s)	75
BssT1I	CCWWGG	2 cut(s)	366, 1462
Bst1107I	GTATAC	1 cut(s)	75
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	4 cut(s)	79, 250, 355, 1028
Bst6I	CTCTTC	2 cut(s)	199, 1305
BstBAI	YACGTR	1 cut(s)	400
BstC8I	GCNNGC	8 cut(s)	446, 927, 969, 1254, 1366, 1370, 1575, 1867
BstDEI	CTNAG	8 cut(s)	532, 987, 1172, 1257, 1416, 1629, 1665, 1945
BstDSI	CCRYGG	2 cut(s)	336, 366
BstENI	CCTNNNNNAGG	1 cut(s)	1145
BstF5I	GGATG	3 cut(s)	550, 1574, 1797
BstFNI	CGCG	3 cut(s)	783, 1331, 1922
BstMAI	GTCTC	8 cut(s)	244, 519, 586, 781, 1034, 1253, 1675, 1717
BstMWI	GCNNNNNNNGC	5 cut(s)	926, 1253, 1337, 1374, 1909
BstNI	CCWGG	1 cut(s)	218
BstNSI	RCATGY	3 cut(s)	448, 760, 898
BstPAI	GACNNNNGTC	1 cut(s)	2130
BstSCI	CCNGG	2 cut(s)	216, 255
BstSFI	CTRYAG	5 cut(s)	115, 288, 690, 1335, 2166
BstUI	CGCG	3 cut(s)	783, 1331, 1922
BstV1I	GCAGC	1 cut(s)	548
BstV2I	GAAGAC	1 cut(s)	2053
BstX2I	RGATCY	1 cut(s)	1159
BstYI	RGATCY	1 cut(s)	1159
BstZ17I	GTATAC	1 cut(s)	75
Bsu36I	CCTNAGG	1 cut(s)	1665
BsuI	GTATCC	1 cut(s)	856
BsuRI	GGCC	4 cut(s)	182, 235, 1083, 1994
BtgI	CCRYGG	2 cut(s)	336, 366
BtrI	CACGTC	1 cut(s)	298
BtsCI	GGATG	3 cut(s)	550, 1574, 1797
BtsI	GCAGTG	2 cut(s)	699, 1435
BtsIMutI	CAGTG	6 cut(s)	351, 660, 699, 1398, 1435, 1973
BtuMI	TCGCGA	1 cut(s)	783
BveI	ACCTGC	1 cut(s)	501
Cac8I	GCNNGC	8 cut(s)	446, 927, 969, 1254, 1366, 1370, 1575, 1867
Cfr13I	GGNCC	4 cut(s)	234, 1082, 1187, 1459
CseI	GACGC	2 cut(s)	665, 1320
Csp6I	GTAC	6 cut(s)	573, 1098, 1281, 1660, 1915, 2066
CviQI	GTAC	6 cut(s)	573, 1098, 1281, 1660, 1915, 2066
DdeI	CTNAG	8 cut(s)	532, 987, 1172, 1257, 1416, 1629, 1665, 1945
DrdI	GACNNNNNNGTC	1 cut(s)	1457
DseDI	GACNNNNNNGTC	1 cut(s)	1457
EaeI	YGGCCR	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	199, 1305
EarI	CTCTTC	2 cut(s)	199, 1305
EciI	GGCGGA	1 cut(s)	1395
Ecl136II	GAGCTC	2 cut(s)	187, 824
Eco130I	CCWWGG	2 cut(s)	366, 1462
Eco24I	GRGCYC	2 cut(s)	189, 826
Eco31I	GGTCTC	3 cut(s)	519, 1253, 1675
Eco47I	GGWCC	2 cut(s)	1187, 1459
Eco53kI	GAGCTC	2 cut(s)	187, 824
Eco72I	CACGTG	1 cut(s)	400
Eco81I	CCTNAGG	1 cut(s)	1665
EcoICRI	GAGCTC	2 cut(s)	187, 824
EcoNI	CCTNNNNNAGG	1 cut(s)	1145
EcoRI	GAATTC	1 cut(s)	1387
EcoRII	CCWGG	1 cut(s)	216
EcoT14I	CCWWGG	2 cut(s)	366, 1462
EcoT22I	ATGCAT	2 cut(s)	30, 733
EcoT38I	GRGCYC	2 cut(s)	189, 826
ErhI	CCWWGG	2 cut(s)	366, 1462
Esp3I	CGTCTC	2 cut(s)	244, 1034
FaqI	GGGAC	1 cut(s)	1480
FauI	CCCGC	1 cut(s)	1915
FbaI	TGATCA	1 cut(s)	855
FblI	GTMKAC	2 cut(s)	74, 294
Fnu4HI	GCNGC	1 cut(s)	537
FokI	GGATG	3 cut(s)	557, 1581, 1804
FriOI	GRGCYC	2 cut(s)	189, 826
Fsp4HI	GCNGC	1 cut(s)	537
FspBI	CTAG	7 cut(s)	720, 968, 1253, 1271, 1365, 1574, 1640
GluI	GCNGC	1 cut(s)	537
GsaI	CCCAGC	1 cut(s)	955
GsuI	CTGGAG	1 cut(s)	840
HaeIII	GGCC	4 cut(s)	182, 235, 1083, 1994
HapII	CCGG	5 cut(s)	257, 269, 320, 849, 1095
HgaI	GACGC	2 cut(s)	665, 1320
HincII	GTYRAC	3 cut(s)	43, 295, 1645
HindII	GTYRAC	3 cut(s)	43, 295, 1645
HindIII	AAGCTT	2 cut(s)	392, 1867
HinfI	GANTC	2 cut(s)	433, 478
HpaII	CCGG	5 cut(s)	257, 269, 320, 849, 1095
HphI	GGTGA	6 cut(s)	355, 572, 668, 715, 1319, 2143
Hpy166II	GTNNAC	4 cut(s)	43, 75, 295, 1645
Hpy188I	TCNGA	5 cut(s)	9, 412, 438, 519, 990
Hpy188III	TCNNGA	8 cut(s)	243, 320, 605, 680, 782, 1114, 1829, 1984
Hpy8I	GTNNAC	4 cut(s)	43, 75, 295, 1645
Hpy99I	CGWCG	3 cut(s)	95, 299, 681
HpyAV	CCTTC	4 cut(s)	319, 804, 880, 2099
HpyCH4III	ACNGT	4 cut(s)	79, 250, 355, 1028
HpyCH4IV	ACGT	5 cut(s)	71, 297, 399, 1043, 1896
HpyF10VI	GCNNNNNNNGC	5 cut(s)	926, 1253, 1337, 1374, 1909
HpyF3I	CTNAG	8 cut(s)	532, 987, 1172, 1257, 1416, 1629, 1665, 1945
HpySE526I	ACGT	5 cut(s)	71, 297, 399, 1043, 1896
Kpn2I	TCCGGA	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	855
LmnI	GCTCC	4 cut(s)	192, 328, 666, 821
Lsp1109I	GCAGC	1 cut(s)	548
LweI	GCATC	3 cut(s)	37, 1763, 1822
MaeI	CTAG	7 cut(s)	720, 968, 1253, 1271, 1365, 1574, 1640
MaeII	ACGT	5 cut(s)	71, 297, 399, 1043, 1896
MaeIII	GTNAC	6 cut(s)	430, 634, 656, 890, 1804, 2149
MflI	RGATCY	1 cut(s)	1159
MhlI	GDGCHC	2 cut(s)	189, 826
MluI	ACGCGT	1 cut(s)	1329
MlyI	GAGTC	1 cut(s)	427
MmeI	TCCRAC	1 cut(s)	1364
Mph1103I	ATGCAT	2 cut(s)	30, 733
MroI	TCCGGA	1 cut(s)	319
MseI	TTAA	2 cut(s)	63, 624
MslI	CAYNNNNRTG	2 cut(s)	226, 1609
MspA1I	CMGCKG	1 cut(s)	314
MspI	CCGG	5 cut(s)	257, 269, 320, 849, 1095
MspR9I	CCNGG	2 cut(s)	218, 257
Mva1269I	GAATGC	2 cut(s)	2115, 2136
MvaI	CCWGG	1 cut(s)	218
MvnI	CGCG	3 cut(s)	783, 1331, 1922
MwoI	GCNNNNNNNGC	5 cut(s)	926, 1253, 1337, 1374, 1909
NciI	CCSGG	1 cut(s)	257
NcoI	CCATGG	1 cut(s)	366
NheI	GCTAGC	4 cut(s)	967, 1252, 1364, 1573
NlaIV	GGNNCC	3 cut(s)	330, 1297, 1613
NmeAIII	GCCGAG	2 cut(s)	208, 901
NmuCI	GTSAC	5 cut(s)	430, 634, 656, 890, 2149
NruI	TCGCGA	1 cut(s)	783
NsiI	ATGCAT	2 cut(s)	30, 733
NspI	RCATGY	3 cut(s)	448, 760, 898
OliI	CACNNNNGTG	1 cut(s)	1609
PaeI	GCATGC	1 cut(s)	448
PciI	ACATGT	1 cut(s)	894
PctI	GAATGC	2 cut(s)	2115, 2136
PfeI	GAWTC	1 cut(s)	478
PfoI	TCCNGGA	1 cut(s)	255
PkrI	GCNGC	1 cut(s)	538
PleI	GAGTC	1 cut(s)	427
PmaCI	CACGTG	1 cut(s)	400
PmlI	CACGTG	1 cut(s)	400
PpsI	GAGTC	1 cut(s)	427
Ppu21I	YACGTR	1 cut(s)	400
PscI	ACATGT	1 cut(s)	894
PshAI	GACNNNNGTC	1 cut(s)	2130
PshBI	ATTAAT	1 cut(s)	63
Psp124BI	GAGCTC	2 cut(s)	189, 826
Psp6I	CCWGG	1 cut(s)	216
PspCI	CACGTG	1 cut(s)	400
PspFI	CCCAGC	1 cut(s)	951
PspGI	CCWGG	1 cut(s)	216
PspN4I	GGNNCC	3 cut(s)	330, 1297, 1613
PspPI	GGNCC	4 cut(s)	234, 1082, 1187, 1459
PstI	CTGCAG	2 cut(s)	292, 694
PsuI	RGATCY	1 cut(s)	1159
RruI	TCGCGA	1 cut(s)	783
RsaI	GTAC	6 cut(s)	574, 1099, 1282, 1661, 1916, 2067
RsaNI	GTAC	6 cut(s)	573, 1098, 1281, 1660, 1915, 2066
RseI	CAYNNNNRTG	2 cut(s)	226, 1609
SacI	GAGCTC	2 cut(s)	189, 826
SalI	GTCGAC	1 cut(s)	293
SaqAI	TTAA	2 cut(s)	63, 624
SatI	GCNGC	1 cut(s)	537
Sau96I	GGNCC	4 cut(s)	234, 1082, 1187, 1459
SchI	GAGTC	1 cut(s)	427
ScrFI	CCNGG	2 cut(s)	218, 257
SduI	GDGCHC	2 cut(s)	189, 826
SfaNI	GCATC	3 cut(s)	37, 1763, 1822
SfcI	CTRYAG	5 cut(s)	115, 288, 690, 1335, 2166
SinI	GGWCC	2 cut(s)	1187, 1459
SmiMI	CAYNNNNRTG	2 cut(s)	226, 1609
SmlI	CTYRAG	1 cut(s)	1907
SmoI	CTYRAG	1 cut(s)	1907
SphI	GCATGC	1 cut(s)	448
SsiI	CCGC	7 cut(s)	312, 420, 472, 1340, 1406, 1912, 1922
SspMI	CTAG	7 cut(s)	720, 968, 1253, 1271, 1365, 1574, 1640
SstI	GAGCTC	2 cut(s)	189, 826
StyD4I	CCNGG	2 cut(s)	216, 255
StyI	CCWWGG	2 cut(s)	366, 1462
TaaI	ACNGT	4 cut(s)	79, 250, 355, 1028
TaiI	ACGT	5 cut(s)	74, 300, 402, 1046, 1899
TaqI	TCGA	7 cut(s)	67, 201, 244, 294, 375, 679, 841
TfiI	GAWTC	1 cut(s)	478
Tru1I	TTAA	2 cut(s)	63, 624
Tru9I	TTAA	2 cut(s)	63, 624
TscAI	CASTG	6 cut(s)	358, 667, 699, 1398, 1435, 1980
TseFI	GTSAC	5 cut(s)	430, 634, 656, 890, 2149
TseI	GCWGC	1 cut(s)	536
Tsp45I	GTSAC	5 cut(s)	430, 634, 656, 890, 2149
TspGWI	ACGGA	2 cut(s)	79, 1763
TspRI	CASTG	6 cut(s)	358, 667, 699, 1398, 1435, 1980
VpaK11BI	GGWCC	2 cut(s)	1187, 1459
VspI	ATTAAT	1 cut(s)	63
XagI	CCTNNNNNAGG	1 cut(s)	1145
XapI	RAATTY	4 cut(s)	992, 1387, 1675, 1847
XceI	RCATGY	3 cut(s)	448, 760, 898
XmiI	GTMKAC	2 cut(s)	74, 294
XspI	CTAG	7 cut(s)	720, 968, 1253, 1271, 1365, 1574, 1640
Zsp2I	ATGCAT	2 cut(s)	30, 733

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.