pycom13g03400

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002115
Physical Location & Seq
Forward (+)
2253796 .. 2255128
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGTGTATATAGGCGGGTCGGAGGAGGAAGAGAGAGTGGCGGCGAAGTCCATGGCGATGGGTTCCGAGAGGACGAAGGCTCTGCACAATTTCAACTTGCCGTGGGATTTGAAGTGGGGGAACCAGAAGCACCTGCGGTGCCAGAAGGAGAGCTCCGACGCCGGCGGGAGTGGCGGGGAAGGGTCGGGGGCTAATCGGCGATCGTCGGCTCAGAGGATGGAGAGCTCGCCGGCGGTGATGACTCGGCGGAGGGATTTGGAGTTCCAGAAGCGGAAGTTTCGGGCCCCGAGGCCGAGGATCGAAAACGACGATGCGGAGGAAGGGATCGATGCGTTTAGGCAGAAGCTCATGTTTGATCTCAAGACGGCGGCGGATAAGATGAAAGACGCGATTTTGAGGGAGAAGGAGGTGGCAGTGGCGGGGGCGGCTGCTGATACTGACGTGGCGGCGGCAGAGGAGGATGTTGAGATGGCGGAGGTTGGCGAATCGGAACCTCCGGTTGCGCCGGAGCCGGAGCAGCCGGAGGCGAGGCCGTGGAATCTGAGGACGAGGAGGGCGGCGTGCAAGGCTCCAATGGGCGGCGGCGGCGGCGGTGCTAAGGTGGTGAGAATCGAGGAAAAGAAGGCGAATTACTCGCCGTTGAGGAGTGAGGGTAACAATGGCGTGAAGTCGCCGAGGCTGGTGAGAGGGGCTTCGGTTTCGGGTCCAGAGAAGAATAAGGAGGAGGAGAAGCCCAAATTCTCGTTGACGCTCACCAAGAAGGAGATCGAGGAAGATTTCATGAAGATGCTCGGCCACCGCCCTCCCCGACGCCCAAAGAGGCGGCCAAGAAATGCTCAGAAGCAATTGGATGGTTTGTTTCCCGGTGCGTGGTTGACGGAGGTCTCGGCGGAGACGTACAAGGTTCCTGAGTTCCCTGAAAATACAAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

312

Amino Acids

34.59

Weight (kDa)

9.25

Isoelectric Point (pI)

73.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 251 - 300 3.1e-26 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 138
AccII CGCG 1 cut(s) 389
AclWI GGATC 2 cut(s) 305, 332
AcoI YGGCCR 2 cut(s) 793, 824
AcsI RAATTY 1 cut(s) 737
AcyI GRCGYC 2 cut(s) 159, 811
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 2 cut(s) 578, 870
AgsI TTSAA 2 cut(s) 94, 112
AjiI CACGTC 1 cut(s) 442
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 3 cut(s) 153, 225, 346
AluI AGCT 3 cut(s) 153, 225, 346
Alw21I GWGCWC 2 cut(s) 155, 227
Alw26I GTCTC 2 cut(s) 887, 889
AlwI GGATC 2 cut(s) 305, 332
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 5 cut(s) 282, 290, 530, 793, 824
ApaI GGGCCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 428, 517
ApoI RAATTY 1 cut(s) 737
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 3 cut(s) 282, 283, 704
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 4 cut(s) 247, 616, 694, 745
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 286
BaeI ACNNNNGTAYC 2 cut(s) 426, 459
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 3 cut(s) 155, 227, 286
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
Bbv12I GWGCWC 2 cut(s) 155, 227
BbvI GCAGC 2 cut(s) 415, 529
BccI CCATC 4 cut(s) 52, 211, 463, 845
BceAI ACGGC 4 cut(s) 85, 381, 517, 622
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 887, 889
BfuAI ACCTGC 1 cut(s) 141
Bme1390I CCNGG 1 cut(s) 864
Bme18I GGWCC 1 cut(s) 704
BmeT110I CYCGRG 1 cut(s) 286
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 3 cut(s) 282, 283, 704
BmrFI CCNGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 301, 319, 777
BoxI GACNNNNGTC 1 cut(s) 881
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 864
Bsa29I ATCGAT 1 cut(s) 327
BsaHI GRCGYC 2 cut(s) 159, 811
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 6 cut(s) 51, 101, 287, 293, 533, 674
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 2 cut(s) 578, 870
Bse118I RCCGGY 2 cut(s) 161, 229
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 6 cut(s) 51, 101, 287, 293, 533, 674
BseGI GGATG 3 cut(s) 222, 466, 856
BseLI CCNNNNNNNGG 2 cut(s) 578, 870
BseMII CTCAG 4 cut(s) 224, 533, 851, 900
BseRI GAGGAG 6 cut(s) 38, 470, 565, 658, 737, 740
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 2 cut(s) 415, 529
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 5 cut(s) 284, 292, 532, 795, 826
BshNI GGYRCC 1 cut(s) 138
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 155, 227
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 7 cut(s) 162, 230, 497, 506, 512, 521, 864
BslI CCNNNNNNNGG 2 cut(s) 578, 870
BsmAI GTCTC 2 cut(s) 887, 889
BsmBI CGTCTC 1 cut(s) 887
BsnI GGCC 5 cut(s) 284, 292, 532, 795, 826
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 286
Bsp120I GGGCCC 1 cut(s) 282
Bsp1286I GDGCHC 3 cut(s) 155, 227, 286
Bsp143I GATC 5 cut(s) 200, 297, 324, 355, 765
Bsp19I CCATGG 1 cut(s) 51
BspANI GGCC 5 cut(s) 284, 292, 532, 795, 826
BspCNI CTCAG 4 cut(s) 223, 534, 850, 901
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 780
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 305, 332
BspT107I GGYRCC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 889
BsrFI RCCGGY 2 cut(s) 161, 229
BssAI RCCGGY 2 cut(s) 161, 229
BssECI CCNNGG 6 cut(s) 51, 101, 287, 293, 533, 674
BssMI GATC 5 cut(s) 200, 297, 324, 355, 765
BssNI GRCGYC 2 cut(s) 159, 811
BssT1I CCWWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 2 cut(s) 159, 811
BstC8I GCNNGC 4 cut(s) 163, 227, 231, 562
BstDEI CTNAG 5 cut(s) 210, 542, 597, 837, 909
BstDSI CCRYGG 3 cut(s) 51, 101, 533
BstF5I GGATG 3 cut(s) 222, 466, 856
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 5 cut(s) 203, 300, 327, 358, 768
BstMAI GTCTC 2 cut(s) 887, 889
BstMBI GATC 5 cut(s) 200, 297, 324, 355, 765
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 6 cut(s) 171, 425, 517, 566, 585, 588
BstPAI GACNNNNGTC 1 cut(s) 881
BstSCI CCNGG 1 cut(s) 862
BstSLI GKGCMC 1 cut(s) 286
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 2 cut(s) 415, 529
BstXI CCANNNNNNTGG 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 5 cut(s) 284, 292, 532, 795, 826
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 3 cut(s) 51, 101, 533
BtgZI GCGATG 1 cut(s) 71
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 3 cut(s) 222, 466, 856
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 163, 227, 231, 562
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 505
Cfr10I RCCGGY 2 cut(s) 161, 229
Cfr13I GGNCC 3 cut(s) 282, 283, 704
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 4 cut(s) 167, 395, 757, 819
Csp6I GTAC 1 cut(s) 898
CviAII CATG 3 cut(s) 52, 349, 781
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 5 cut(s) 210, 542, 597, 837, 909
DpnI GATC 5 cut(s) 202, 299, 326, 357, 767
DpnII GATC 5 cut(s) 200, 297, 324, 355, 765
EaeI YGGCCR 2 cut(s) 793, 824
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 4 cut(s) 262, 386, 488, 905
Ecl136II GAGCTC 2 cut(s) 153, 225
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 3 cut(s) 155, 227, 286
Eco31I GGTCTC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 704
Eco53kI GAGCTC 2 cut(s) 153, 225
Eco88I CYCGRG 1 cut(s) 286
EcoICRI GAGCTC 2 cut(s) 153, 225
EcoO109I RGGNCCY 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 3 cut(s) 155, 227, 286
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 887
FaeI CATG 3 cut(s) 55, 352, 784
FaiI YATR 5 cut(s) 9, 11, 53, 350, 782
FatI CATG 3 cut(s) 51, 348, 780
FauI CCCGC 4 cut(s) 8, 158, 167, 412
FokI GGATG 3 cut(s) 229, 473, 863
FriOI GRGCYC 3 cut(s) 155, 227, 286
GlaI GCGC 1 cut(s) 504
HaeIII GGCC 5 cut(s) 284, 292, 532, 795, 826
HapII CCGG 7 cut(s) 162, 230, 497, 506, 512, 521, 864
HgaI GACGC 4 cut(s) 167, 395, 757, 819
HhaI GCGC 1 cut(s) 505
Hin1I GRCGYC 2 cut(s) 159, 811
Hin1II CATG 3 cut(s) 55, 352, 784
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HincII GTYRAC 2 cut(s) 747, 876
HindII GTYRAC 2 cut(s) 747, 876
HinfI GANTC 4 cut(s) 241, 485, 538, 609
HpaII CCGG 7 cut(s) 162, 230, 497, 506, 512, 521, 864
HphI GGTGA 4 cut(s) 247, 616, 694, 745
Hpy166II GTNNAC 2 cut(s) 747, 876
Hpy188I TCNGA 7 cut(s) 22, 67, 157, 213, 490, 543, 840
Hpy188III TCNNGA 5 cut(s) 265, 361, 707, 781, 908
Hpy8I GTNNAC 2 cut(s) 747, 876
Hpy99I CGWCG 4 cut(s) 161, 208, 311, 813
HpyAV CCTTC 7 cut(s) 70, 139, 173, 314, 397, 616, 754
HpyCH4IV ACGT 2 cut(s) 441, 896
HpyCH4V TGCA 2 cut(s) 85, 564
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 425, 517, 566, 585, 588
HpyF3I CTNAG 5 cut(s) 210, 542, 597, 837, 909
HpySE526I ACGT 2 cut(s) 441, 896
Hsp92I GRCGYC 2 cut(s) 159, 811
Hsp92II CATG 3 cut(s) 55, 352, 784
HspAI GCGC 1 cut(s) 503
KroI GCCGGC 2 cut(s) 161, 229
KroNI GCCGGC 2 cut(s) 163, 231
Kzo9I GATC 5 cut(s) 200, 297, 324, 355, 765
LmnI GCTCC 4 cut(s) 158, 508, 514, 574
Lsp1109I GCAGC 2 cut(s) 415, 529
LweI GCATC 3 cut(s) 301, 319, 777
MaeII ACGT 2 cut(s) 441, 896
MaeIII GTNAC 1 cut(s) 653
MalI GATC 5 cut(s) 202, 299, 326, 357, 767
MboI GATC 5 cut(s) 200, 297, 324, 355, 765
MboII GAAGA 4 cut(s) 41, 724, 785, 796
MfeI CAATTG 1 cut(s) 845
MhlI GDGCHC 3 cut(s) 155, 227, 286
MluCI AATT 4 cut(s) 88, 628, 737, 845
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 1 cut(s) 180
MreI CGCCGGCG 2 cut(s) 161, 229
MroNI GCCGGC 2 cut(s) 161, 229
MslI CAYNNNNRTG 2 cut(s) 56, 785
MspI CCGG 7 cut(s) 162, 230, 497, 506, 512, 521, 864
MspR9I CCNGG 1 cut(s) 864
MunI CAATTG 1 cut(s) 845
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 6 cut(s) 171, 425, 517, 566, 585, 588
NaeI GCCGGC 2 cut(s) 163, 231
NciI CCSGG 1 cut(s) 864
NcoI CCATGG 1 cut(s) 51
NdeII GATC 5 cut(s) 200, 297, 324, 355, 765
NgoMIV GCCGGC 2 cut(s) 161, 229
NlaIII CATG 3 cut(s) 55, 352, 784
NmeAIII GCCGAG 5 cut(s) 223, 318, 699, 771, 866
PagI TCATGA 1 cut(s) 780
PaqCI CACCTGC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 893
PdiI GCCGGC 2 cut(s) 163, 231
PfeI GAWTC 3 cut(s) 485, 538, 609
Ple19I CGATCG 1 cut(s) 203
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 881
Psp124BI GAGCTC 2 cut(s) 155, 227
PspOMI GGGCCC 1 cut(s) 282
PspPI GGNCC 3 cut(s) 282, 283, 704
PvuI CGATCG 1 cut(s) 203
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
RseI CAYNNNNRTG 2 cut(s) 56, 785
SacI GAGCTC 2 cut(s) 155, 227
Sau3AI GATC 5 cut(s) 200, 297, 324, 355, 765
Sau96I GGNCC 3 cut(s) 282, 283, 704
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 864
SduI GDGCHC 3 cut(s) 155, 227, 286
SfaNI GCATC 3 cut(s) 301, 319, 777
SgrAI CRCCGGYG 2 cut(s) 161, 229
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 56, 785
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 4 cut(s) 88, 628, 737, 845
SstI GAGCTC 2 cut(s) 155, 227
StyD4I CCNGG 1 cut(s) 862
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 444, 899
TaqI TCGA 4 cut(s) 300, 327, 612, 768
TasI AATT 4 cut(s) 88, 628, 737, 845
TfiI GAWTC 3 cut(s) 485, 538, 609
TscAI CASTG 1 cut(s) 420
TseI GCWGC 2 cut(s) 428, 517
TspDTI ATGAA 3 cut(s) 395, 769, 797
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 1 cut(s) 420
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.