pycom15g30700

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000945
Physical Location & Seq
Reverse (-)
28550053 .. 28550985
933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g30700.1

Sequence Viewer

Length: 585 bp
ATGAGTGAAAACACTGCCACAAGGAACCAGCTGCAGCTTCATCACAACCCAGTTTTTGATGTCTCACTCAATGTGAATGGAGGCTCCAAGTACATTGATGACGATGGCCGTCCCAAACGAACCGGAACTGTTTGGACTGCAAGTTCACACATAATTACTGCTGTAATTGGGTCTGGAGTTCTGTCATTGGCTTGGGCCATAGCTCAGCTTGGATGGATTGCTGGTCCTGCTGCGATGGCCATCTTCTCCTTCATCACTTACTACACTTCAACTCTTCTGGCTTCTTGCTATCGTGATCCCGTCACTGGAAAGAGAAACTACAGTTACTCCGATGCCGTTCAATCCAACCTTGGTGAATTTCAGGTCAAAATGTGTGGATTTGTTCAGCAATTGAACCTTTTTGGAATTACTATTGGCTATACTGTAGCAGCATCTATAAGCATGATGGCAATAAAGAGGTCTAATTGCTTCCACAATAGTGGTGGAAAATTACCCAATGATCGATCCATGTCATATCAACTACAATCCCTATATGATAGCTTTTGGAACCTCAGAAATTATATTGTCTCAAATTCCAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

195

Amino Acids

21.39

Weight (kDa)

9.17

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 40 - 162 4.2e-38 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 290, 498
AcoI YGGCCR 2 cut(s) 106, 237
AcsI RAATTY 2 cut(s) 356, 571
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 3 cut(s) 270, 341, 394
AleI CACNNNNGTG 1 cut(s) 477
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
AluBI AGCT 5 cut(s) 31, 37, 203, 208, 540
AluI AGCT 5 cut(s) 31, 37, 203, 208, 540
Alw26I GTCTC 2 cut(s) 67, 571
AlwI GGATC 2 cut(s) 290, 498
AoxI GGCC 3 cut(s) 106, 195, 237
ApeKI GCWGC 4 cut(s) 31, 34, 230, 428
ApoI RAATTY 2 cut(s) 356, 571
AspS9I GGNCC 2 cut(s) 195, 224
AsuHPI GGTGA 1 cut(s) 365
AvaII GGWCC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 239
BbvI GCAGC 4 cut(s) 18, 46, 217, 440
BccI CCATC 5 cut(s) 98, 207, 229, 248, 439
BceAI ACGGC 2 cut(s) 93, 320
BcoDI GTCTC 2 cut(s) 67, 571
BfmI CTRYAG 3 cut(s) 32, 319, 423
BisI GCNGC 4 cut(s) 32, 35, 231, 429
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 4 cut(s) 33, 36, 232, 430
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 195, 224
BmiI GGNNCC 3 cut(s) 26, 85, 548
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 322, 440
BmuI ACTGGG 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 204
Bsa29I ATCGAT 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 349
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 2 cut(s) 50, 310
Bse8I GATNNNNATC 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 349
BseGI GGATG 1 cut(s) 218
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 2 cut(s) 218, 565
BseNI ACTGG 2 cut(s) 50, 310
BseXI GCAGC 4 cut(s) 18, 46, 217, 440
BshFI GGCC 3 cut(s) 108, 197, 239
BshVI ATCGAT 1 cut(s) 502
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 67, 571
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 108, 197, 239
Bsp143I GATC 3 cut(s) 295, 499, 503
Bsp1720I GCTNAGC 1 cut(s) 204
BspANI GGCC 3 cut(s) 108, 197, 239
BspCNI CTCAG 2 cut(s) 217, 564
BspDI ATCGAT 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 26, 85, 548
BspMAI CTGCAG 1 cut(s) 36
BspPI GGATC 2 cut(s) 290, 498
BsrI ACTGG 2 cut(s) 50, 310
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 3 cut(s) 295, 499, 503
BssT1I CCWWGG 1 cut(s) 349
Bst4CI ACNGT 3 cut(s) 130, 323, 424
Bst6I CTCTTC 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 204, 551
BstF5I GGATG 1 cut(s) 218
BstKTI GATC 3 cut(s) 298, 502, 506
BstMAI GTCTC 2 cut(s) 67, 571
BstMBI GATC 3 cut(s) 295, 499, 503
BstMWI GCNNNNNNNGC 2 cut(s) 227, 236
BstSFI CTRYAG 3 cut(s) 32, 319, 423
BstV1I GCAGC 4 cut(s) 18, 46, 217, 440
BstXI CCANNNNNNTGG 1 cut(s) 479
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 3 cut(s) 108, 197, 239
BsuTUI ATCGAT 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 248
BtsCI GGATG 1 cut(s) 218
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 2 cut(s) 12, 303
Cfr13I GGNCC 2 cut(s) 195, 224
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 1 cut(s) 91
CspCI CAANNNNNGTGG 4 cut(s) 355, 390, 463, 498
CviAII CATG 2 cut(s) 442, 508
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 204, 551
DpnI GATC 3 cut(s) 297, 501, 505
DpnII GATC 3 cut(s) 295, 499, 503
EaeI YGGCCR 2 cut(s) 106, 237
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 445, 511
FaqI GGGAC 1 cut(s) 96
FatI CATG 2 cut(s) 441, 507
Fnu4HI GCNGC 4 cut(s) 32, 35, 231, 429
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 4 cut(s) 32, 35, 231, 429
GluI GCNGC 4 cut(s) 32, 35, 231, 429
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 3 cut(s) 108, 197, 239
HapII CCGG 1 cut(s) 123
Hin1II CATG 2 cut(s) 445, 511
HpaII CCGG 1 cut(s) 123
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 2 cut(s) 331, 554
Hpy188III TCNNGA 2 cut(s) 174, 293
Hpy8I GTNNAC 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 3 cut(s) 130, 323, 424
HpyCH4V TGCA 2 cut(s) 34, 140
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 236
HpyF3I CTNAG 2 cut(s) 204, 551
Hsp92II CATG 2 cut(s) 445, 511
Kzo9I GATC 3 cut(s) 295, 499, 503
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 9 cut(s) 41, 63, 136, 159, 207, 240, 263, 291, 347
Lsp1109I GCAGC 4 cut(s) 18, 46, 217, 440
LweI GCATC 2 cut(s) 322, 440
MaeIII GTNAC 2 cut(s) 301, 323
MalI GATC 3 cut(s) 297, 501, 505
MboI GATC 3 cut(s) 295, 499, 503
MboII GAAGA 2 cut(s) 235, 266
MfeI CAATTG 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 9 cut(s) 153, 165, 356, 389, 405, 463, 488, 556, 571
MluNI TGGCCA 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 3 cut(s) 74, 450, 560
Mox20I TGGCCA 1 cut(s) 239
MscI TGGCCA 1 cut(s) 239
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 389
MwoI GCNNNNNNNGC 2 cut(s) 227, 236
NdeII GATC 3 cut(s) 295, 499, 503
NlaIII CATG 2 cut(s) 445, 511
NlaIV GGNNCC 3 cut(s) 26, 85, 548
NmuCI GTSAC 1 cut(s) 301
OliI CACNNNNGTG 1 cut(s) 477
PkrI GCNGC 4 cut(s) 33, 36, 232, 430
PspN4I GGNNCC 3 cut(s) 26, 85, 548
PspPI GGNCC 2 cut(s) 195, 224
PstI CTGCAG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 31
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 477
SatI GCNGC 4 cut(s) 32, 35, 231, 429
Sau3AI GATC 3 cut(s) 295, 499, 503
Sau96I GGNCC 2 cut(s) 195, 224
SfaNI GCATC 2 cut(s) 322, 440
SfcI CTRYAG 3 cut(s) 32, 319, 423
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 9 cut(s) 153, 165, 356, 389, 405, 463, 488, 556, 571
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 3 cut(s) 130, 323, 424
TaqI TCGA 1 cut(s) 502
TasI AATT 9 cut(s) 153, 165, 356, 389, 405, 463, 488, 556, 571
TatI WGTACW 1 cut(s) 90
TscAI CASTG 2 cut(s) 19, 310
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 4 cut(s) 31, 34, 230, 428
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 29, 241
TspRI CASTG 2 cut(s) 19, 310
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 2 cut(s) 356, 571
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.