eukaryotic translation initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 4 cut(s) | 1376, 2228, 3083, 3938 |
| Acc36I | ACCTGC | 1 cut(s) | 610 |
| AccB1I | GGYRCC | 3 cut(s) | 113, 119, 272 |
| AccBSI | CCGCTC | 1 cut(s) | 4417 |
| AccI | GTMKAC | 1 cut(s) | 4109 |
| AccII | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| AclWI | GGATC | 5 cut(s) | 54, 224, 237, 4449, 5174 |
| AcuI | CTGAAG | 5 cut(s) | 1074, 1926, 2778, 3633, 4614 |
| AcyI | GRCGYC | 3 cut(s) | 114, 120, 273 |
| AfaI | GTAC | 4 cut(s) | 264, 282, 4597, 5140 |
| AflIII | ACRYGT | 1 cut(s) | 4592 |
| AgeI | ACCGGT | 4 cut(s) | 821, 1673, 2525, 3380 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 143, 175 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 5106, 5138 |
| Alw44I | GTGCAC | 4 cut(s) | 1433, 2285, 3140, 3995 |
| AlwI | GGATC | 5 cut(s) | 54, 224, 237, 4449, 5174 |
| Ama87I | CYCGRG | 2 cut(s) | 369, 4695 |
| ApaI | GGGCCC | 6 cut(s) | 132, 432, 1521, 2373, 3228, 4083 |
| ApaLI | GTGCAC | 4 cut(s) | 1433, 2285, 3140, 3995 |
| AseI | ATTAAT | 5 cut(s) | 1112, 1964, 2819, 3674, 5015 |
| AsiGI | ACCGGT | 4 cut(s) | 821, 1673, 2525, 3380 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 4896 |
| AspA2I | CCTAGG | 2 cut(s) | 197, 4433 |
| AspLEI | GCGC | 9 cut(s) | 116, 122, 255, 275, 573, 1377, 2229, 3084, 3939 |
| AsuC2I | CCSGG | 1 cut(s) | 4957 |
| AsuII | TTCGAA | 1 cut(s) | 4566 |
| AvaI | CYCGRG | 2 cut(s) | 369, 4695 |
| AvrII | CCTAGG | 2 cut(s) | 197, 4433 |
| AxyI | CCTNAGG | 1 cut(s) | 108 |
| BamHI | GGATCC | 1 cut(s) | 229 |
| BanI | GGYRCC | 3 cut(s) | 113, 119, 272 |
| BanII | GRGCYC | 7 cut(s) | 71, 132, 432, 1521, 2373, 3228, 4083 |
| BarI | GAAGNNNNNNTAC | 8 cut(s) | 872, 904, 1724, 1756, 2576, 2608, 3431, 3463 |
| BauI | CACGAG | 3 cut(s) | 1608, 2460, 3315 |
| BbsI | GAAGAC | 4 cut(s) | 936, 1788, 2640, 3495 |
| BceAI | ACGGC | 6 cut(s) | 599, 1467, 2319, 3174, 4029, 4904 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 5059, 5093 |
| BciVI | GTATCC | 5 cut(s) | 83, 1354, 2206, 3061, 3916 |
| BclI | TGATCA | 5 cut(s) | 1258, 2110, 2965, 3820, 4495 |
| BcnI | CCSGG | 1 cut(s) | 4957 |
| BfoI | RGCGCY | 3 cut(s) | 117, 123, 276 |
| BfuAI | ACCTGC | 1 cut(s) | 610 |
| BfuI | GTATCC | 5 cut(s) | 83, 1354, 2206, 3061, 3916 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 1022, 1874, 2726, 3581 |
| BglII | AGATCT | 2 cut(s) | 4571, 4810 |
| BlnI | CCTAGG | 2 cut(s) | 197, 4433 |
| BmeT110I | CYCGRG | 2 cut(s) | 369, 4695 |
| BoxI | GACNNNNGTC | 1 cut(s) | 824 |
| BpiI | GAAGAC | 4 cut(s) | 936, 1788, 2640, 3495 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 313, 345, 352, 384 |
| BpmI | CTGGAG | 9 cut(s) | 372, 740, 1182, 2034, 2889, 3744, 4354, 4383, 5160 |
| Bpu14I | TTCGAA | 1 cut(s) | 4566 |
| BpuEI | CTTGAG | 1 cut(s) | 406 |
| BpuMI | CCSGG | 1 cut(s) | 4957 |
| BsaAI | YACGTR | 1 cut(s) | 4595 |
| BsaHI | GRCGYC | 3 cut(s) | 114, 120, 273 |
| BsaWI | WCCGGW | 4 cut(s) | 821, 1673, 2525, 3380 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 5106, 5136 |
| Bse118I | RCCGGY | 5 cut(s) | 116, 821, 1673, 2525, 3380 |
| Bse21I | CCTNAGG | 1 cut(s) | 108 |
| Bse3DI | GCAATG | 2 cut(s) | 611, 5077 |
| BseMI | GCAATG | 2 cut(s) | 611, 5077 |
| BseMII | CTCAG | 5 cut(s) | 410, 653, 660, 4335, 4425 |
| BseRI | GAGGAG | 9 cut(s) | 136, 1046, 1411, 1898, 2263, 2750, 3118, 3605, 5048 |
| BseYI | CCCAGC | 6 cut(s) | 125, 1477, 2329, 3184, 4039, 4872 |
| BsgI | GTGCAG | 6 cut(s) | 588, 1431, 2283, 3138, 3993, 4218 |
| Bsh1236I | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| BshNI | GGYRCC | 3 cut(s) | 113, 119, 272 |
| BshTI | ACCGGT | 4 cut(s) | 821, 1673, 2525, 3380 |
| BsiHKCI | CYCGRG | 2 cut(s) | 369, 4695 |
| BslFI | GGGAC | 5 cut(s) | 434, 4278, 4553, 4831, 4855 |
| BsmBI | CGTCTC | 8 cut(s) | 29, 399, 835, 1084, 1936, 2788, 3643, 4692 |
| BsmFI | GGGAC | 5 cut(s) | 434, 4278, 4553, 4831, 4855 |
| BsoBI | CYCGRG | 2 cut(s) | 369, 4695 |
| Bsp119I | TTCGAA | 1 cut(s) | 4566 |
| Bsp120I | GGGCCC | 6 cut(s) | 128, 428, 1517, 2369, 3224, 4079 |
| Bsp1407I | TGTACA | 1 cut(s) | 280 |
| Bsp19I | CCATGG | 3 cut(s) | 1524, 2376, 3231 |
| BspCNI | CTCAG | 5 cut(s) | 409, 652, 661, 4336, 4424 |
| BspFNI | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| BspMI | ACCTGC | 1 cut(s) | 610 |
| BspPI | GGATC | 5 cut(s) | 54, 224, 237, 4449, 5174 |
| BspT104I | TTCGAA | 1 cut(s) | 4566 |
| BspT107I | GGYRCC | 3 cut(s) | 113, 119, 272 |
| BsrBI | CCGCTC | 1 cut(s) | 4417 |
| BsrDI | GCAATG | 2 cut(s) | 611, 5077 |
| BsrFI | RCCGGY | 5 cut(s) | 116, 821, 1673, 2525, 3380 |
| BsrGI | TGTACA | 1 cut(s) | 280 |
| BssAI | RCCGGY | 5 cut(s) | 116, 821, 1673, 2525, 3380 |
| BssNI | GRCGYC | 3 cut(s) | 114, 120, 273 |
| BssSI | CACGAG | 3 cut(s) | 1608, 2460, 3315 |
| Bst2BI | CACGAG | 3 cut(s) | 1608, 2460, 3315 |
| Bst6I | CTCTTC | 5 cut(s) | 1062, 1914, 2766, 3621, 4820 |
| BstACI | GRCGYC | 3 cut(s) | 114, 120, 273 |
| BstAUI | TGTACA | 1 cut(s) | 280 |
| BstBAI | YACGTR | 1 cut(s) | 4595 |
| BstBI | TTCGAA | 1 cut(s) | 4566 |
| BstC8I | GCNNGC | 5 cut(s) | 67, 118, 132, 5064, 5068 |
| BstDEI | CTNAG | 8 cut(s) | 108, 396, 465, 639, 669, 4344, 4411, 4652 |
| BstENI | CCTNNNNNAGG | 4 cut(s) | 952, 1804, 2656, 3511 |
| BstFNI | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| BstH2I | RGCGCY | 3 cut(s) | 117, 123, 276 |
| BstHHI | GCGC | 9 cut(s) | 116, 122, 255, 275, 573, 1377, 2229, 3084, 3939 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 122, 131, 1022, 1874, 2726, 3581, 4937 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 824 |
| BstUI | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| BstV2I | GAAGAC | 4 cut(s) | 936, 1788, 2640, 3495 |
| BstX2I | RGATCY | 5 cut(s) | 229, 4441, 4571, 4810, 5179 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 4288, 4378 |
| BstYI | RGATCY | 5 cut(s) | 229, 4441, 4571, 4810, 5179 |
| Bsu36I | CCTNAGG | 1 cut(s) | 108 |
| BsuI | GTATCC | 5 cut(s) | 83, 1354, 2206, 3061, 3916 |
| BveI | ACCTGC | 1 cut(s) | 610 |
| Cac8I | GCNNGC | 5 cut(s) | 67, 118, 132, 5064, 5068 |
| CfoI | GCGC | 9 cut(s) | 116, 122, 255, 275, 573, 1377, 2229, 3084, 3939 |
| Cfr10I | RCCGGY | 5 cut(s) | 116, 821, 1673, 2525, 3380 |
| Cfr42I | CCGCGG | 4 cut(s) | 909, 1761, 2613, 3468 |
| CseI | GACGC | 3 cut(s) | 46, 381, 450 |
| Csp6I | GTAC | 4 cut(s) | 263, 281, 4596, 5139 |
| CspAI | ACCGGT | 4 cut(s) | 821, 1673, 2525, 3380 |
| CviQI | GTAC | 4 cut(s) | 263, 281, 4596, 5139 |
| DdeI | CTNAG | 8 cut(s) | 108, 396, 465, 639, 669, 4344, 4411, 4652 |
| DinI | GGCGCC | 3 cut(s) | 115, 121, 274 |
| Eam1104I | CTCTTC | 5 cut(s) | 1062, 1914, 2766, 3621, 4820 |
| EarI | CTCTTC | 5 cut(s) | 1062, 1914, 2766, 3621, 4820 |
| Ecl136II | GAGCTC | 1 cut(s) | 69 |
| Eco24I | GRGCYC | 7 cut(s) | 71, 132, 432, 1521, 2373, 3228, 4083 |
| Eco32I | GATATC | 2 cut(s) | 4350, 4764 |
| Eco53kI | GAGCTC | 1 cut(s) | 69 |
| Eco57I | CTGAAG | 5 cut(s) | 1074, 1926, 2778, 3633, 4614 |
| Eco81I | CCTNAGG | 1 cut(s) | 108 |
| Eco88I | CYCGRG | 2 cut(s) | 369, 4695 |
| EcoICRI | GAGCTC | 1 cut(s) | 69 |
| EcoNI | CCTNNNNNAGG | 4 cut(s) | 952, 1804, 2656, 3511 |
| EcoRI | GAATTC | 1 cut(s) | 412 |
| EcoRV | GATATC | 2 cut(s) | 4350, 4764 |
| EcoT22I | ATGCAT | 9 cut(s) | 1161, 1291, 2013, 2143, 2868, 2998, 3723, 3853, 4724 |
| EcoT38I | GRGCYC | 7 cut(s) | 71, 132, 432, 1521, 2373, 3228, 4083 |
| EgeI | GGCGCC | 3 cut(s) | 115, 121, 274 |
| EheI | GGCGCC | 3 cut(s) | 115, 121, 274 |
| Esp3I | CGTCTC | 8 cut(s) | 29, 399, 835, 1084, 1936, 2788, 3643, 4692 |
| FaqI | GGGAC | 5 cut(s) | 434, 4278, 4553, 4831, 4855 |
| FauI | CCCGC | 3 cut(s) | 106, 139, 4410 |
| FbaI | TGATCA | 5 cut(s) | 1258, 2110, 2965, 3820, 4495 |
| FblI | GTMKAC | 1 cut(s) | 4109 |
| FriOI | GRGCYC | 7 cut(s) | 71, 132, 432, 1521, 2373, 3228, 4083 |
| FspI | TGCGCA | 4 cut(s) | 1376, 2228, 3083, 3938 |
| GlaI | GCGC | 9 cut(s) | 115, 121, 254, 274, 572, 1376, 2228, 3083, 3938 |
| GsaI | CCCAGC | 6 cut(s) | 129, 1481, 2333, 3188, 4043, 4876 |
| GsuI | CTGGAG | 9 cut(s) | 372, 740, 1182, 2034, 2889, 3744, 4354, 4383, 5160 |
| HaeII | RGCGCY | 3 cut(s) | 117, 123, 276 |
| HgaI | GACGC | 3 cut(s) | 46, 381, 450 |
| HhaI | GCGC | 9 cut(s) | 116, 122, 255, 275, 573, 1377, 2229, 3084, 3939 |
| Hin1I | GRCGYC | 3 cut(s) | 114, 120, 273 |
| Hin6I | GCGC | 9 cut(s) | 114, 120, 253, 273, 571, 1375, 2227, 3082, 3937 |
| HinP1I | GCGC | 9 cut(s) | 114, 120, 253, 273, 571, 1375, 2227, 3082, 3937 |
| HincII | GTYRAC | 5 cut(s) | 1143, 1995, 2850, 3705, 4110 |
| HindII | GTYRAC | 5 cut(s) | 1143, 1995, 2850, 3705, 4110 |
| HindIII | AAGCTT | 6 cut(s) | 1218, 2070, 2925, 3780, 4744, 4823 |
| HpyCH4IV | ACGT | 1 cut(s) | 4594 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 122, 131, 1022, 1874, 2726, 3581, 4937 |
| HpyF3I | CTNAG | 8 cut(s) | 108, 396, 465, 639, 669, 4344, 4411, 4652 |
| HpySE526I | ACGT | 1 cut(s) | 4594 |
| Hsp92I | GRCGYC | 3 cut(s) | 114, 120, 273 |
| HspAI | GCGC | 9 cut(s) | 114, 120, 253, 273, 571, 1375, 2227, 3082, 3937 |
| KasI | GGCGCC | 3 cut(s) | 113, 119, 272 |
| KflI | GGGWCCC | 2 cut(s) | 4292, 4540 |
| KroI | GCCGGC | 1 cut(s) | 116 |
| KroNI | GCCGGC | 1 cut(s) | 118 |
| Ksp22I | TGATCA | 5 cut(s) | 1258, 2110, 2965, 3820, 4495 |
| KspI | CCGCGG | 4 cut(s) | 909, 1761, 2613, 3468 |
| MaeII | ACGT | 1 cut(s) | 4594 |
| MaeIII | GTNAC | 5 cut(s) | 1100, 1952, 2807, 3662, 4774 |
| MbiI | CCGCTC | 1 cut(s) | 4417 |
| MfeI | CAATTG | 8 cut(s) | 1242, 1365, 2094, 2217, 2949, 3072, 3804, 3927 |
| MflI | RGATCY | 5 cut(s) | 229, 4441, 4571, 4810, 5179 |
| Mly113I | GGCGCC | 3 cut(s) | 114, 120, 273 |
| MmeI | TCCRAC | 5 cut(s) | 174, 4620, 4778, 5139, 5172 |
| Mph1103I | ATGCAT | 9 cut(s) | 1161, 1291, 2013, 2143, 2868, 2998, 3723, 3853, 4724 |
| MreI | CGCCGGCG | 1 cut(s) | 116 |
| MroNI | GCCGGC | 1 cut(s) | 116 |
| MroXI | GAANNNNTTC | 1 cut(s) | 4896 |
| MspA1I | CMGCKG | 9 cut(s) | 125, 258, 908, 1760, 2612, 3467, 4334, 4385, 4487 |
| MunI | CAATTG | 8 cut(s) | 1242, 1365, 2094, 2217, 2949, 3072, 3804, 3927 |
| MvnI | CGCG | 7 cut(s) | 65, 253, 573, 908, 1760, 2612, 3467 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 122, 131, 1022, 1874, 2726, 3581, 4937 |
| NaeI | GCCGGC | 1 cut(s) | 118 |
| NarI | GGCGCC | 3 cut(s) | 114, 120, 273 |
| NciI | CCSGG | 1 cut(s) | 4957 |
| NcoI | CCATGG | 3 cut(s) | 1524, 2376, 3231 |
| NgoMIV | GCCGGC | 1 cut(s) | 116 |
| NmeAIII | GCCGAG | 1 cut(s) | 521 |
| NmuCI | GTSAC | 1 cut(s) | 4774 |
| NsbI | TGCGCA | 4 cut(s) | 1376, 2228, 3083, 3938 |
| NsiI | ATGCAT | 9 cut(s) | 1161, 1291, 2013, 2143, 2868, 2998, 3723, 3853, 4724 |
| NspV | TTCGAA | 1 cut(s) | 4566 |
| PaeR7I | CTCGAG | 1 cut(s) | 369 |
| PasI | CCCWGGG | 1 cut(s) | 4297 |
| PdiI | GCCGGC | 1 cut(s) | 118 |
| PdmI | GAANNNNTTC | 1 cut(s) | 4896 |
| PfeI | GAWTC | 8 cut(s) | 523, 1498, 2350, 3205, 4060, 4277, 4999, 5134 |
| PflFI | GACNNNGTC | 1 cut(s) | 4243 |
| PfoI | TCCNGGA | 2 cut(s) | 4360, 4370 |
| PinAI | ACCGGT | 4 cut(s) | 821, 1673, 2525, 3380 |
| PluTI | GGCGCC | 3 cut(s) | 117, 123, 276 |
| Ppu21I | YACGTR | 1 cut(s) | 4595 |
| PpuMI | RGGWCCY | 4 cut(s) | 448, 4239, 4292, 4540 |
| PshAI | GACNNNNGTC | 1 cut(s) | 824 |
| PshBI | ATTAAT | 5 cut(s) | 1112, 1964, 2819, 3674, 5015 |
| Psp124BI | GAGCTC | 1 cut(s) | 71 |
| Psp5II | RGGWCCY | 4 cut(s) | 448, 4239, 4292, 4540 |
| PspFI | CCCAGC | 6 cut(s) | 125, 1477, 2329, 3184, 4039, 4872 |
| PspOMI | GGGCCC | 6 cut(s) | 128, 428, 1517, 2369, 3224, 4079 |
| PspPPI | RGGWCCY | 4 cut(s) | 448, 4239, 4292, 4540 |
| PsuI | RGATCY | 5 cut(s) | 229, 4441, 4571, 4810, 5179 |
| PsyI | GACNNNGTC | 1 cut(s) | 4243 |
| PvuII | CAGCTG | 3 cut(s) | 4334, 4385, 4487 |
| RsaI | GTAC | 4 cut(s) | 264, 282, 4597, 5140 |
| RsaNI | GTAC | 4 cut(s) | 263, 281, 4596, 5139 |
| SacI | GAGCTC | 1 cut(s) | 71 |
| SacII | CCGCGG | 4 cut(s) | 909, 1761, 2613, 3468 |
| SalI | GTCGAC | 1 cut(s) | 4108 |
| SfoI | GGCGCC | 3 cut(s) | 115, 121, 274 |
| Sfr274I | CTCGAG | 1 cut(s) | 369 |
| Sfr303I | CCGCGG | 4 cut(s) | 909, 1761, 2613, 3468 |
| SfuI | TTCGAA | 1 cut(s) | 4566 |
| SgrAI | CRCCGGYG | 1 cut(s) | 116 |
| SgrBI | CCGCGG | 4 cut(s) | 909, 1761, 2613, 3468 |
| SlaI | CTCGAG | 1 cut(s) | 369 |
| SmlI | CTYRAG | 2 cut(s) | 369, 421 |
| SmoI | CTYRAG | 2 cut(s) | 369, 421 |
| SspDI | GGCGCC | 3 cut(s) | 113, 119, 272 |
| SspI | AATATT | 7 cut(s) | 799, 1423, 1651, 2275, 2503, 3130, 3358 |
| SstI | GAGCTC | 1 cut(s) | 71 |
| TaiI | ACGT | 1 cut(s) | 4597 |
| TaqI | TCGA | 8 cut(s) | 370, 532, 1316, 2168, 3023, 3878, 4109, 4566 |
| TaqII | GACCGA | 4 cut(s) | 1160, 2012, 2867, 3722 |
| TatI | WGTACW | 1 cut(s) | 280 |
| TauI | GCSGC | 7 cut(s) | 125, 289, 908, 1760, 2612, 3467, 4465 |
| TfiI | GAWTC | 8 cut(s) | 523, 1498, 2350, 3205, 4060, 4277, 4999, 5134 |
| TseFI | GTSAC | 1 cut(s) | 4774 |
| Tsp45I | GTSAC | 1 cut(s) | 4774 |
| Tth111I | GACNNNGTC | 1 cut(s) | 4243 |
| VneI | GTGCAC | 4 cut(s) | 1433, 2285, 3140, 3995 |
| VspI | ATTAAT | 5 cut(s) | 1112, 1964, 2819, 3674, 5015 |
| XagI | CCTNNNNNAGG | 4 cut(s) | 952, 1804, 2656, 3511 |
| XcmI | CCANNNNNNNNNTGG | 5 cut(s) | 1279, 2131, 2986, 3841, 5155 |
| XhoI | CTCGAG | 1 cut(s) | 369 |
| XmaJI | CCTAGG | 2 cut(s) | 197, 4433 |
| XmiI | GTMKAC | 1 cut(s) | 4109 |
| XmnI | GAANNNNTTC | 1 cut(s) | 4896 |
| Zsp2I | ATGCAT | 9 cut(s) | 1161, 1291, 2013, 2143, 2868, 2998, 3723, 3853, 4724 |