eukaryotic translation initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 300 |
| AccI | GTMKAC | 1 cut(s) | 67 |
| AclWI | GGATC | 1 cut(s) | 257 |
| AcsI | RAATTY | 2 cut(s) | 104, 246 |
| AcuI | CTGAAG | 2 cut(s) | 105, 330 |
| AfaI | GTAC | 1 cut(s) | 214 |
| AgsI | TTSAA | 2 cut(s) | 95, 222 |
| AluBI | AGCT | 1 cut(s) | 375 |
| AluI | AGCT | 1 cut(s) | 375 |
| AlwI | GGATC | 1 cut(s) | 257 |
| ApeKI | GCWGC | 1 cut(s) | 375 |
| ApoI | RAATTY | 2 cut(s) | 104, 246 |
| BbsI | GAAGAC | 1 cut(s) | 318 |
| BbvI | GCAGC | 1 cut(s) | 362 |
| BclI | TGATCA | 1 cut(s) | 283 |
| BfaI | CTAG | 4 cut(s) | 15, 99, 207, 351 |
| BisI | GCNGC | 1 cut(s) | 376 |
| BlsI | GCNGC | 1 cut(s) | 377 |
| BmcAI | AGTACT | 1 cut(s) | 214 |
| BmrI | ACTGGG | 1 cut(s) | 57 |
| BmsI | GCATC | 1 cut(s) | 338 |
| BmuI | ACTGGG | 1 cut(s) | 57 |
| BpiI | GAAGAC | 1 cut(s) | 318 |
| BsaJI | CCNNGG | 1 cut(s) | 174 |
| Bse1I | ACTGG | 1 cut(s) | 63 |
| BseDI | CCNNGG | 1 cut(s) | 174 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BseMII | CTCAG | 1 cut(s) | 188 |
| BseNI | ACTGG | 1 cut(s) | 63 |
| BseXI | GCAGC | 1 cut(s) | 362 |
| BseYI | CCCAGC | 1 cut(s) | 269 |
| Bsp143I | GATC | 3 cut(s) | 73, 262, 283 |
| BspCNI | CTCAG | 1 cut(s) | 189 |
| BspPI | GGATC | 1 cut(s) | 257 |
| BsrI | ACTGG | 1 cut(s) | 63 |
| BssECI | CCNNGG | 1 cut(s) | 174 |
| BssMI | GATC | 3 cut(s) | 73, 262, 283 |
| BssT1I | CCWWGG | 1 cut(s) | 174 |
| Bst4CI | ACNGT | 1 cut(s) | 163 |
| Bst6I | CTCTTC | 3 cut(s) | 16, 192, 230 |
| BstDEI | CTNAG | 2 cut(s) | 197, 255 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstKTI | GATC | 3 cut(s) | 76, 265, 286 |
| BstMBI | GATC | 3 cut(s) | 73, 262, 283 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 266 |
| BstV1I | GCAGC | 1 cut(s) | 362 |
| BstV2I | GAAGAC | 1 cut(s) | 318 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| BtsIMutI | CAGTG | 1 cut(s) | 168 |
| Csp6I | GTAC | 1 cut(s) | 213 |
| CviAII | CATG | 2 cut(s) | 31, 168 |
| CviJI | RGCY | 4 cut(s) | 79, 260, 296, 375 |
| CviKI_1 | RGCY | 4 cut(s) | 79, 260, 296, 375 |
| CviQI | GTAC | 1 cut(s) | 213 |
| DdeI | CTNAG | 2 cut(s) | 197, 255 |
| DpnI | GATC | 3 cut(s) | 75, 264, 285 |
| DpnII | GATC | 3 cut(s) | 73, 262, 283 |
| Eam1104I | CTCTTC | 3 cut(s) | 16, 192, 230 |
| EarI | CTCTTC | 3 cut(s) | 16, 192, 230 |
| Eco130I | CCWWGG | 1 cut(s) | 174 |
| Eco57I | CTGAAG | 2 cut(s) | 105, 330 |
| EcoT14I | CCWWGG | 1 cut(s) | 174 |
| ErhI | CCWWGG | 1 cut(s) | 174 |
| FaeI | CATG | 2 cut(s) | 34, 171 |
| FaiI | YATR | 8 cut(s) | 32, 129, 169, 203, 291, 300, 326, 356 |
| FatI | CATG | 2 cut(s) | 30, 167 |
| FbaI | TGATCA | 1 cut(s) | 283 |
| FblI | GTMKAC | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 1 cut(s) | 376 |
| FokI | GGATG | 1 cut(s) | 17 |
| Fsp4HI | GCNGC | 1 cut(s) | 376 |
| FspBI | CTAG | 4 cut(s) | 15, 99, 207, 351 |
| GluI | GCNGC | 1 cut(s) | 376 |
| GsaI | CCCAGC | 1 cut(s) | 273 |
| Hin1II | CATG | 2 cut(s) | 34, 171 |
| HincII | GTYRAC | 1 cut(s) | 68 |
| HindII | GTYRAC | 1 cut(s) | 68 |
| HinfI | GANTC | 1 cut(s) | 152 |
| Hpy166II | GTNNAC | 1 cut(s) | 68 |
| Hpy188I | TCNGA | 4 cut(s) | 124, 283, 337, 364 |
| Hpy188III | TCNNGA | 1 cut(s) | 99 |
| Hpy8I | GTNNAC | 1 cut(s) | 68 |
| HpyCH4III | ACNGT | 1 cut(s) | 163 |
| HpyCH4IV | ACGT | 1 cut(s) | 278 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 266 |
| HpyF3I | CTNAG | 2 cut(s) | 197, 255 |
| HpySE526I | ACGT | 1 cut(s) | 278 |
| Hsp92II | CATG | 2 cut(s) | 34, 171 |
| Ksp22I | TGATCA | 1 cut(s) | 283 |
| Kzo9I | GATC | 3 cut(s) | 73, 262, 283 |
| LpnPI | CCDG | 7 cut(s) | 76, 84, 96, 255, 279, 321, 354 |
| Lsp1109I | GCAGC | 1 cut(s) | 362 |
| LweI | GCATC | 1 cut(s) | 338 |
| MaeI | CTAG | 4 cut(s) | 15, 99, 207, 351 |
| MaeII | ACGT | 1 cut(s) | 278 |
| MaeIII | GTNAC | 1 cut(s) | 274 |
| MalI | GATC | 3 cut(s) | 75, 264, 285 |
| MboI | GATC | 3 cut(s) | 73, 262, 283 |
| MboII | GAAGA | 8 cut(s) | 3, 179, 241, 243, 244, 247, 323, 324 |
| MluCI | AATT | 2 cut(s) | 104, 246 |
| MnlI | CCTC | 2 cut(s) | 19, 91 |
| MseI | TTAA | 1 cut(s) | 56 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 266 |
| NdeII | GATC | 3 cut(s) | 73, 262, 283 |
| NlaIII | CATG | 2 cut(s) | 34, 171 |
| PfeI | GAWTC | 1 cut(s) | 152 |
| PkrI | GCNGC | 1 cut(s) | 377 |
| PsiI | TTATAA | 1 cut(s) | 300 |
| PspFI | CCCAGC | 1 cut(s) | 269 |
| RsaI | GTAC | 1 cut(s) | 214 |
| RsaNI | GTAC | 1 cut(s) | 213 |
| SalI | GTCGAC | 1 cut(s) | 66 |
| SaqAI | TTAA | 1 cut(s) | 56 |
| SatI | GCNGC | 1 cut(s) | 376 |
| Sau3AI | GATC | 3 cut(s) | 73, 262, 283 |
| ScaI | AGTACT | 1 cut(s) | 214 |
| SetI | ASST | 2 cut(s) | 281, 377 |
| SfaNI | GCATC | 1 cut(s) | 338 |
| Sse9I | AATT | 2 cut(s) | 104, 246 |
| SspMI | CTAG | 4 cut(s) | 15, 99, 207, 351 |
| StyI | CCWWGG | 1 cut(s) | 174 |
| TaaI | ACNGT | 1 cut(s) | 163 |
| TaiI | ACGT | 1 cut(s) | 281 |
| TaqI | TCGA | 1 cut(s) | 67 |
| TasI | AATT | 2 cut(s) | 104, 246 |
| TatI | WGTACW | 1 cut(s) | 212 |
| TfiI | GAWTC | 1 cut(s) | 152 |
| Tru1I | TTAA | 1 cut(s) | 56 |
| Tru9I | TTAA | 1 cut(s) | 56 |
| TscAI | CASTG | 1 cut(s) | 168 |
| TseI | GCWGC | 1 cut(s) | 375 |
| TspRI | CASTG | 1 cut(s) | 168 |
| XapI | RAATTY | 2 cut(s) | 104, 246 |
| XbaI | TCTAGA | 1 cut(s) | 98 |
| XmiI | GTMKAC | 1 cut(s) | 67 |
| XspI | CTAG | 4 cut(s) | 15, 99, 207, 351 |
| ZrmI | AGTACT | 1 cut(s) | 214 |