eukaryotic translation initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2811 |
| AarI | CACCTGC | 1 cut(s) | 1679 |
| Acc16I | TGCGCA | 1 cut(s) | 4238 |
| Acc36I | ACCTGC | 3 cut(s) | 610, 1679, 3395 |
| AccB1I | GGYRCC | 4 cut(s) | 113, 119, 272, 2117 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 782, 1006, 1196, 3209 |
| AccBSI | CCGCTC | 1 cut(s) | 4732 |
| AccI | GTMKAC | 4 cut(s) | 669, 1533, 2533, 4409 |
| AccII | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| AclWI | GGATC | 9 cut(s) | 54, 224, 237, 794, 1637, 3379, 3392, 4764, 5489 |
| AcoI | YGGCCR | 1 cut(s) | 2040 |
| AcyI | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| AjiI | CACGTC | 1 cut(s) | 3257 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 143, 175, 2216, 2248, 3391, 3423 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 5421, 5453 |
| Alw21I | GWGCWC | 9 cut(s) | 71, 1075, 2364, 2864, 2941, 4103, 4143, 4299, 5257 |
| Alw26I | GTCTC | 6 cut(s) | 29, 302, 733, 3943, 4674, 5007 |
| Alw44I | GTGCAC | 3 cut(s) | 1071, 2937, 4295 |
| AlwI | GGATC | 9 cut(s) | 54, 224, 237, 794, 1637, 3379, 3392, 4764, 5489 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1006, 1559, 2006 |
| Ama87I | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| ApaI | GGGCCC | 3 cut(s) | 132, 432, 4381 |
| ApaLI | GTGCAC | 3 cut(s) | 1071, 2937, 4295 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 4687, 4719 |
| AseI | ATTAAT | 2 cut(s) | 3974, 5330 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 241, 2106, 5028, 5211 |
| AspA2I | CCTAGG | 2 cut(s) | 197, 4748 |
| AspLEI | GCGC | 6 cut(s) | 116, 255, 275, 573, 2120, 4239 |
| AsuC2I | CCSGG | 2 cut(s) | 3682, 5272 |
| AsuII | TTCGAA | 1 cut(s) | 4881 |
| AsuNHI | GCTAGC | 1 cut(s) | 746 |
| AvaI | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| AvrII | CCTAGG | 2 cut(s) | 197, 4748 |
| AxyI | CCTNAGG | 1 cut(s) | 108 |
| BaeGI | GKGCMC | 8 cut(s) | 132, 432, 1075, 2941, 4299, 4381, 4825, 5011 |
| BalI | TGGCCA | 1 cut(s) | 2042 |
| BamHI | GGATCC | 2 cut(s) | 229, 3384 |
| BanI | GGYRCC | 4 cut(s) | 113, 119, 272, 2117 |
| BanII | GRGCYC | 5 cut(s) | 71, 132, 432, 2958, 4381 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 229, 261, 1960, 1992, 3731, 3763 |
| BauI | CACGAG | 2 cut(s) | 4022, 4470 |
| BbsI | GAAGAC | 3 cut(s) | 2829, 3250, 3795 |
| Bbv12I | GWGCWC | 9 cut(s) | 71, 1075, 2364, 2864, 2941, 4103, 4143, 4299, 5257 |
| BbvCI | CCTCAGC | 3 cut(s) | 687, 1032, 1773 |
| BceAI | ACGGC | 3 cut(s) | 599, 4329, 5219 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 5374, 5408 |
| BciVI | GTATCC | 4 cut(s) | 83, 220, 2270, 4216 |
| BclI | TGATCA | 2 cut(s) | 4120, 4810 |
| BcnI | CCSGG | 2 cut(s) | 3682, 5272 |
| BcoDI | GTCTC | 6 cut(s) | 29, 302, 733, 3943, 4674, 5007 |
| BfmI | CTRYAG | 4 cut(s) | 827, 1686, 1986, 2919 |
| BfoI | RGCGCY | 3 cut(s) | 117, 276, 2121 |
| BfuAI | ACCTGC | 3 cut(s) | 610, 1679, 3395 |
| BfuI | GTATCC | 4 cut(s) | 83, 220, 2270, 4216 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1591, 3881 |
| BglII | AGATCT | 2 cut(s) | 4886, 5125 |
| BlnI | CCTAGG | 2 cut(s) | 197, 4748 |
| BlpI | GCTNAGC | 2 cut(s) | 2952, 3369 |
| BmcAI | AGTACT | 3 cut(s) | 1918, 2146, 2722 |
| BmeT110I | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| BmgBI | CACGTC | 1 cut(s) | 3257 |
| BmrI | ACTGGG | 1 cut(s) | 2157 |
| BmtI | GCTAGC | 1 cut(s) | 750 |
| BmuI | ACTGGG | 1 cut(s) | 2157 |
| BpiI | GAAGAC | 3 cut(s) | 2829, 3250, 3795 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 352, 384 |
| BpmI | CTGGAG | 6 cut(s) | 372, 1620, 4044, 4669, 4698, 5475 |
| Bpu10I | CCTNAGC | 4 cut(s) | 633, 687, 1032, 1773 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 2952, 3369 |
| Bpu14I | TTCGAA | 1 cut(s) | 4881 |
| BpuEI | CTTGAG | 2 cut(s) | 406, 909 |
| BpuMI | CCSGG | 2 cut(s) | 3682, 5272 |
| BsaAI | YACGTR | 2 cut(s) | 4910, 5336 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1185 |
| BsaHI | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 5421, 5451 |
| Bse118I | RCCGGY | 1 cut(s) | 116 |
| Bse1I | ACTGG | 4 cut(s) | 1667, 1787, 2152, 3003 |
| Bse21I | CCTNAGG | 1 cut(s) | 108 |
| Bse3DI | GCAATG | 5 cut(s) | 611, 1538, 1661, 2398, 5392 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1185 |
| BseJI | GATNNNNATC | 1 cut(s) | 1185 |
| BseMI | GCAATG | 5 cut(s) | 611, 1538, 1661, 2398, 5392 |
| BseNI | ACTGG | 4 cut(s) | 1667, 1787, 2152, 3003 |
| BseRI | GAGGAG | 7 cut(s) | 136, 1790, 2116, 2119, 3905, 4273, 5363 |
| BseSI | GKGCMC | 8 cut(s) | 132, 432, 1075, 2941, 4299, 4381, 4825, 5011 |
| BseYI | CCCAGC | 6 cut(s) | 125, 1239, 1768, 2780, 4339, 5187 |
| BsgI | GTGCAG | 5 cut(s) | 302, 588, 2400, 4293, 4518 |
| Bsh1236I | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| BshNI | GGYRCC | 4 cut(s) | 113, 119, 272, 2117 |
| BsiHKAI | GWGCWC | 9 cut(s) | 71, 1075, 2364, 2864, 2941, 4103, 4143, 4299, 5257 |
| BsiHKCI | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| BsiSI | CCGG | 5 cut(s) | 117, 3681, 4466, 5272, 5485 |
| BslFI | GGGAC | 6 cut(s) | 434, 3375, 4578, 4868, 5146, 5170 |
| BsmAI | GTCTC | 6 cut(s) | 29, 302, 733, 3943, 4674, 5007 |
| BsmBI | CGTCTC | 3 cut(s) | 29, 3943, 5007 |
| BsmFI | GGGAC | 6 cut(s) | 434, 3375, 4578, 4868, 5146, 5170 |
| BsmI | GAATGC | 1 cut(s) | 3175 |
| BsoBI | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| Bsp119I | TTCGAA | 1 cut(s) | 4881 |
| Bsp120I | GGGCCC | 3 cut(s) | 128, 428, 4377 |
| Bsp1407I | TGTACA | 1 cut(s) | 1471 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 2952, 3369 |
| Bsp19I | CCATGG | 1 cut(s) | 4386 |
| BspFNI | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| BspHI | TCATGA | 1 cut(s) | 1425 |
| BspMAI | CTGCAG | 3 cut(s) | 831, 1690, 2923 |
| BspMI | ACCTGC | 3 cut(s) | 610, 1679, 3395 |
| BspOI | GCTAGC | 1 cut(s) | 750 |
| BspPI | GGATC | 9 cut(s) | 54, 224, 237, 794, 1637, 3379, 3392, 4764, 5489 |
| BspT104I | TTCGAA | 1 cut(s) | 4881 |
| BspT107I | GGYRCC | 4 cut(s) | 113, 119, 272, 2117 |
| BsrBI | CCGCTC | 1 cut(s) | 4732 |
| BsrDI | GCAATG | 5 cut(s) | 611, 1538, 1661, 2398, 5392 |
| BsrFI | RCCGGY | 1 cut(s) | 116 |
| BsrGI | TGTACA | 1 cut(s) | 1471 |
| BsrI | ACTGG | 4 cut(s) | 1667, 1787, 2152, 3003 |
| BssAI | RCCGGY | 1 cut(s) | 116 |
| BssNI | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| BssSI | CACGAG | 2 cut(s) | 4022, 4470 |
| Bst2BI | CACGAG | 2 cut(s) | 4022, 4470 |
| Bst4CI | ACNGT | 9 cut(s) | 217, 609, 1519, 2253, 2620, 2671, 3633, 3909, 5006 |
| Bst6I | CTCTTC | 5 cut(s) | 1149, 2137, 2482, 3921, 5135 |
| BstACI | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| BstAUI | TGTACA | 1 cut(s) | 1471 |
| BstBAI | YACGTR | 2 cut(s) | 4910, 5336 |
| BstBI | TTCGAA | 1 cut(s) | 4881 |
| BstC8I | GCNNGC | 7 cut(s) | 67, 132, 748, 1108, 1596, 5379, 5383 |
| BstDSI | CCRYGG | 6 cut(s) | 3028, 3356, 3765, 4345, 4386, 4450 |
| BstEII | GGTNACC | 1 cut(s) | 2785 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 3116, 3321, 3811 |
| BstFNI | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| BstH2I | RGCGCY | 3 cut(s) | 117, 276, 2121 |
| BstHHI | GCGC | 6 cut(s) | 116, 255, 275, 573, 2120, 4239 |
| BstMAI | GTCTC | 6 cut(s) | 29, 302, 733, 3943, 4674, 5007 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 122, 131, 689, 1591, 2777, 3881, 4376, 5252 |
| BstNSI | RCATGY | 5 cut(s) | 1357, 1463, 1955, 2441, 3377 |
| BstPI | GGTNACC | 1 cut(s) | 2785 |
| BstSFI | CTRYAG | 4 cut(s) | 827, 1686, 1986, 2919 |
| BstSLI | GKGCMC | 8 cut(s) | 132, 432, 1075, 2941, 4299, 4381, 4825, 5011 |
| BstSNI | TACGTA | 1 cut(s) | 4910 |
| BstUI | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| BstV2I | GAAGAC | 3 cut(s) | 2829, 3250, 3795 |
| BstX2I | RGATCY | 7 cut(s) | 229, 786, 3384, 4756, 4886, 5125, 5494 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 4588 |
| BstYI | RGATCY | 7 cut(s) | 229, 786, 3384, 4756, 4886, 5125, 5494 |
| Bsu36I | CCTNAGG | 1 cut(s) | 108 |
| BsuI | GTATCC | 4 cut(s) | 83, 220, 2270, 4216 |
| BtgI | CCRYGG | 6 cut(s) | 3028, 3356, 3765, 4345, 4386, 4450 |
| BtrI | CACGTC | 1 cut(s) | 3257 |
| BtsI | GCAGTG | 3 cut(s) | 1645, 2073, 2204 |
| BveI | ACCTGC | 3 cut(s) | 610, 1679, 3395 |
| Cac8I | GCNNGC | 7 cut(s) | 67, 132, 748, 1108, 1596, 5379, 5383 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1006, 1559, 2006 |
| CciI | TCATGA | 1 cut(s) | 1425 |
| CfoI | GCGC | 6 cut(s) | 116, 255, 275, 573, 2120, 4239 |
| Cfr10I | RCCGGY | 1 cut(s) | 116 |
| Cfr42I | CCGCGG | 1 cut(s) | 3768 |
| CseI | GACGC | 2 cut(s) | 46, 450 |
| DinI | GGCGCC | 3 cut(s) | 115, 274, 2119 |
| EaeI | YGGCCR | 1 cut(s) | 2040 |
| Eam1104I | CTCTTC | 5 cut(s) | 1149, 2137, 2482, 3921, 5135 |
| EarI | CTCTTC | 5 cut(s) | 1149, 2137, 2482, 3921, 5135 |
| EciI | GGCGGA | 1 cut(s) | 3302 |
| Ecl136II | GAGCTC | 1 cut(s) | 69 |
| Eco105I | TACGTA | 1 cut(s) | 4910 |
| Eco24I | GRGCYC | 5 cut(s) | 71, 132, 432, 2958, 4381 |
| Eco32I | GATATC | 2 cut(s) | 4665, 5079 |
| Eco53kI | GAGCTC | 1 cut(s) | 69 |
| Eco81I | CCTNAGG | 1 cut(s) | 108 |
| Eco88I | CYCGRG | 3 cut(s) | 369, 1938, 5010 |
| Eco91I | GGTNACC | 1 cut(s) | 2785 |
| EcoICRI | GAGCTC | 1 cut(s) | 69 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 3116, 3321, 3811 |
| EcoO109I | RGGNCCY | 9 cut(s) | 428, 448, 1028, 1109, 4377, 4378, 4539, 4592, 4855 |
| EcoO65I | GGTNACC | 1 cut(s) | 2785 |
| EcoRI | GAATTC | 4 cut(s) | 412, 1327, 1574, 2197 |
| EcoRV | GATATC | 2 cut(s) | 4665, 5079 |
| EcoT22I | ATGCAT | 3 cut(s) | 993, 4153, 5039 |
| EcoT38I | GRGCYC | 5 cut(s) | 71, 132, 432, 2958, 4381 |
| EgeI | GGCGCC | 3 cut(s) | 115, 274, 2119 |
| EheI | GGCGCC | 3 cut(s) | 115, 274, 2119 |
| Esp3I | CGTCTC | 3 cut(s) | 29, 3943, 5007 |
| FaqI | GGGAC | 6 cut(s) | 434, 3375, 4578, 4868, 5146, 5170 |
| FauI | CCCGC | 3 cut(s) | 106, 139, 4725 |
| FbaI | TGATCA | 2 cut(s) | 4120, 4810 |
| FblI | GTMKAC | 4 cut(s) | 669, 1533, 2533, 4409 |
| FriOI | GRGCYC | 5 cut(s) | 71, 132, 432, 2958, 4381 |
| FspI | TGCGCA | 1 cut(s) | 4238 |
| GlaI | GCGC | 6 cut(s) | 115, 254, 274, 572, 2119, 4238 |
| GsaI | CCCAGC | 6 cut(s) | 129, 1243, 1772, 2784, 4343, 5191 |
| GsuI | CTGGAG | 6 cut(s) | 372, 1620, 4044, 4669, 4698, 5475 |
| HaeII | RGCGCY | 3 cut(s) | 117, 276, 2121 |
| HapII | CCGG | 5 cut(s) | 117, 3681, 4466, 5272, 5485 |
| HgaI | GACGC | 2 cut(s) | 46, 450 |
| HhaI | GCGC | 6 cut(s) | 116, 255, 275, 573, 2120, 4239 |
| Hin1I | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| Hin6I | GCGC | 6 cut(s) | 114, 253, 273, 571, 2118, 4237 |
| HinP1I | GCGC | 6 cut(s) | 114, 253, 273, 571, 2118, 4237 |
| HincII | GTYRAC | 6 cut(s) | 670, 1534, 1654, 2347, 4005, 4410 |
| HindII | GTYRAC | 6 cut(s) | 670, 1534, 1654, 2347, 4005, 4410 |
| HindIII | AAGCTT | 3 cut(s) | 4080, 5059, 5138 |
| HpaI | GTTAAC | 1 cut(s) | 1654 |
| HpaII | CCGG | 5 cut(s) | 117, 3681, 4466, 5272, 5485 |
| Hpy99I | CGWCG | 1 cut(s) | 3258 |
| HpyCH4III | ACNGT | 9 cut(s) | 217, 609, 1519, 2253, 2620, 2671, 3633, 3909, 5006 |
| HpyCH4IV | ACGT | 5 cut(s) | 3134, 3256, 4909, 4963, 5335 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 122, 131, 689, 1591, 2777, 3881, 4376, 5252 |
| HpySE526I | ACGT | 5 cut(s) | 3134, 3256, 4909, 4963, 5335 |
| Hsp92I | GRCGYC | 3 cut(s) | 114, 273, 2118 |
| HspAI | GCGC | 6 cut(s) | 114, 253, 273, 571, 2118, 4237 |
| KasI | GGCGCC | 3 cut(s) | 113, 272, 2117 |
| KflI | GGGWCCC | 2 cut(s) | 4592, 4855 |
| Ksp22I | TGATCA | 2 cut(s) | 4120, 4810 |
| KspAI | GTTAAC | 1 cut(s) | 1654 |
| KspI | CCGCGG | 1 cut(s) | 3768 |
| MaeII | ACGT | 5 cut(s) | 3134, 3256, 4909, 4963, 5335 |
| MbiI | CCGCTC | 1 cut(s) | 4732 |
| MfeI | CAATTG | 3 cut(s) | 3399, 4104, 4227 |
| MflI | RGATCY | 7 cut(s) | 229, 786, 3384, 4756, 4886, 5125, 5494 |
| MlsI | TGGCCA | 1 cut(s) | 2042 |
| MluNI | TGGCCA | 1 cut(s) | 2042 |
| Mly113I | GGCGCC | 3 cut(s) | 114, 273, 2118 |
| MmeI | TCCRAC | 6 cut(s) | 174, 2235, 4935, 5093, 5454, 5487 |
| Mox20I | TGGCCA | 1 cut(s) | 2042 |
| Mph1103I | ATGCAT | 3 cut(s) | 993, 4153, 5039 |
| MroXI | GAANNNNTTC | 4 cut(s) | 241, 2106, 5028, 5211 |
| MscI | TGGCCA | 1 cut(s) | 2042 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1224, 4146, 4302, 4527 |
| Msp20I | TGGCCA | 1 cut(s) | 2042 |
| MspA1I | CMGCKG | 9 cut(s) | 125, 1036, 1529, 1598, 1772, 3767, 4649, 4700, 4802 |
| MspI | CCGG | 5 cut(s) | 117, 3681, 4466, 5272, 5485 |
| MunI | CAATTG | 3 cut(s) | 3399, 4104, 4227 |
| Mva1269I | GAATGC | 1 cut(s) | 3175 |
| MvnI | CGCG | 5 cut(s) | 65, 253, 255, 573, 3767 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 122, 131, 689, 1591, 2777, 3881, 4376, 5252 |
| NarI | GGCGCC | 3 cut(s) | 114, 273, 2118 |
| NciI | CCSGG | 2 cut(s) | 3682, 5272 |
| NcoI | CCATGG | 1 cut(s) | 4386 |
| NheI | GCTAGC | 1 cut(s) | 746 |
| NmeAIII | GCCGAG | 1 cut(s) | 521 |
| NmuCI | GTSAC | 5 cut(s) | 858, 1349, 2071, 3032, 5089 |
| NsbI | TGCGCA | 1 cut(s) | 4238 |
| NsiI | ATGCAT | 3 cut(s) | 993, 4153, 5039 |
| NspI | RCATGY | 5 cut(s) | 1357, 1463, 1955, 2441, 3377 |
| NspV | TTCGAA | 1 cut(s) | 4881 |
| PaeR7I | CTCGAG | 1 cut(s) | 369 |
| PagI | TCATGA | 1 cut(s) | 1425 |
| PaqCI | CACCTGC | 1 cut(s) | 1679 |
| PasI | CCCWGGG | 1 cut(s) | 4597 |
| PctI | GAATGC | 1 cut(s) | 3175 |
| PdmI | GAANNNNTTC | 4 cut(s) | 241, 2106, 5028, 5211 |
| PflFI | GACNNNGTC | 1 cut(s) | 4543 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 782, 1006, 1196, 3209 |
| PfoI | TCCNGGA | 5 cut(s) | 232, 979, 3208, 4675, 4685 |
| PluTI | GGCGCC | 3 cut(s) | 117, 276, 2121 |
| Ppu21I | YACGTR | 2 cut(s) | 4910, 5336 |
| PpuMI | RGGWCCY | 5 cut(s) | 448, 1028, 4539, 4592, 4855 |
| PshBI | ATTAAT | 2 cut(s) | 3974, 5330 |
| PsiI | TTATAA | 1 cut(s) | 2811 |
| Psp124BI | GAGCTC | 1 cut(s) | 71 |
| Psp5II | RGGWCCY | 5 cut(s) | 448, 1028, 4539, 4592, 4855 |
| PspEI | GGTNACC | 1 cut(s) | 2785 |
| PspFI | CCCAGC | 6 cut(s) | 125, 1239, 1768, 2780, 4339, 5187 |
| PspOMI | GGGCCC | 3 cut(s) | 128, 428, 4377 |
| PspPPI | RGGWCCY | 5 cut(s) | 448, 1028, 4539, 4592, 4855 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 74, 106, 2704, 2736 |
| PstI | CTGCAG | 3 cut(s) | 831, 1690, 2923 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1006, 1559, 2006 |
| PsuI | RGATCY | 7 cut(s) | 229, 786, 3384, 4756, 4886, 5125, 5494 |
| PsyI | GACNNNGTC | 1 cut(s) | 4543 |
| PvuII | CAGCTG | 7 cut(s) | 1036, 1529, 1598, 1772, 4649, 4700, 4802 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1224, 4146, 4302, 4527 |
| SacI | GAGCTC | 1 cut(s) | 71 |
| SacII | CCGCGG | 1 cut(s) | 3768 |
| SalI | GTCGAC | 3 cut(s) | 668, 1532, 4408 |
| ScaI | AGTACT | 3 cut(s) | 1918, 2146, 2722 |
| SfcI | CTRYAG | 4 cut(s) | 827, 1686, 1986, 2919 |
| SfoI | GGCGCC | 3 cut(s) | 115, 274, 2119 |
| Sfr274I | CTCGAG | 1 cut(s) | 369 |
| Sfr303I | CCGCGG | 1 cut(s) | 3768 |
| SfuI | TTCGAA | 1 cut(s) | 4881 |
| SgrAI | CRCCGGYG | 1 cut(s) | 116 |
| SgrBI | CCGCGG | 1 cut(s) | 3768 |
| SlaI | CTCGAG | 1 cut(s) | 369 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1224, 4146, 4302, 4527 |
| SmlI | CTYRAG | 3 cut(s) | 369, 421, 924 |
| SmoI | CTYRAG | 3 cut(s) | 369, 421, 924 |
| SnaBI | TACGTA | 1 cut(s) | 4910 |
| SspDI | GGCGCC | 3 cut(s) | 113, 272, 2117 |
| SspI | AATATT | 3 cut(s) | 2635, 2644, 3658 |
| SstI | GAGCTC | 1 cut(s) | 71 |
| TaaI | ACNGT | 9 cut(s) | 217, 609, 1519, 2253, 2620, 2671, 3633, 3909, 5006 |
| TaiI | ACGT | 5 cut(s) | 3137, 3259, 4912, 4966, 5338 |
| TaqII | GACCGA | 3 cut(s) | 1821, 2059, 4022 |
| TatI | WGTACW | 5 cut(s) | 928, 1471, 1916, 2144, 2720 |
| TauI | GCSGC | 6 cut(s) | 125, 289, 1211, 1699, 3767, 4780 |
| TseFI | GTSAC | 5 cut(s) | 858, 1349, 2071, 3032, 5089 |
| Tsp45I | GTSAC | 5 cut(s) | 858, 1349, 2071, 3032, 5089 |
| TspGWI | ACGGA | 4 cut(s) | 312, 3961, 4439, 4496 |
| Tth111I | GACNNNGTC | 1 cut(s) | 4543 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 782, 1006, 1196, 3209 |
| VneI | GTGCAC | 3 cut(s) | 1071, 2937, 4295 |
| VspI | ATTAAT | 2 cut(s) | 3974, 5330 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 3116, 3321, 3811 |
| XceI | RCATGY | 5 cut(s) | 1357, 1463, 1955, 2441, 3377 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 1091, 3329, 4141, 5470 |
| XhoI | CTCGAG | 1 cut(s) | 369 |
| XmaJI | CCTAGG | 2 cut(s) | 197, 4748 |
| XmiI | GTMKAC | 4 cut(s) | 669, 1533, 2533, 4409 |
| XmnI | GAANNNNTTC | 4 cut(s) | 241, 2106, 5028, 5211 |
| ZrmI | AGTACT | 3 cut(s) | 1918, 2146, 2722 |
| Zsp2I | ATGCAT | 3 cut(s) | 993, 4153, 5039 |