Rorug05G0171200

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000945
Physical Location & Seq
Reverse (-)
15948250 .. 15954202
5953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0171200.1

Sequence Viewer

Length: 2184 bp
ATGATTCTCTCTCTAACTCTCGCAAGAGAGGTAGCTTGGCGGCGGCTAGGTTTTCCTGGTGACGGCTCTGTACTCTCACCCAAGAAGGGAGTCTCTTCACCTCCCTCTCGGTTGTTCCTTGGTAACGGCGGGCGTGCTCGACGATGCCTGTATTCTGTAGGCTTCGGCCTTATTTTCTTTGCTGCGTTCGTGGTTTGCTTTCGCGGCAATGGTGGTGTGGCTGATAAGGAGGTGATCTGGGGTCATCCTGATGGGTCTCAATGGATGGTTTTTGCAAAGGTCACCGCTAGTGATTGGGTTGCTGCTTGGATGACAGATCCAGATCAGAGTGCTCTGGGGATGCTGGAGGCAAGGTCTGGCATTGGTGTATGCTCAGATTGCGGCGAAGAACGTTGGCAGAATTGGGGTGATCTCGGACTTATGGGGATCTGCTCGTCGTCGGAGATGTTTCGCCAAGAATCGGGTCCGCGAGCTACCAGTGGTGGTGGATGCACGGGCGTGGGTCTCTGGCGAGTGGTGCATGGTGGCGGTGGCAGGGTTGTCTTCTCACTTGGTGTTTGTCCTAGGTGGGTTGCCAGGGACTGGTATTGGGATGTCCTCATCTCAGGTGACGGAATCTGGAGAACAGTGGTGAAGGTTTTCCAAAGCGGTGGTTGCGCAGTCGGTGTCCTCCGGCGGGTGTTTGGTGGCGGCGGCAGGGCTGCTTGCTCACTTAGGGTTTGTCCTAGGTGGTTGGGTTGGGCCTGGAATGTGACTGTTAGGGTGCCAATTTGGGCCCTGGGACAGCCGGACAGGTTTAGGGTTTATGAATCCGACCAATTACTATTTGGGAAAACCGGGTTTGCCCCATACCCGGTAGCAACCTTAGAAGACGCCCGCCTGATAAAGTCTCTGTCTACTTCTACTTCCTTACCATGCACCACCACTACCCTTCAAGAACTTGCTTCTGCTCACCCCAAAGGTGTTGCGAGGGTTGTACTCAAGAAGGGGAAGACACAACTATTCAAGGATGGAAGTCCAATGGTTTATAGTGGAGCAGTTGATAGAATAATTGGGAGACCGCCACCAAGAACTGGAGATGTTGTGCTAGTAGCTGATGGAGCAGAAAAGCCAATAGGGTGGGGCATGTATAATTCAGTCTCCATGTTCTGTGTCCGGCTTATGCAGCTGGATGAGGAAGCAATAAGGGATTGTTCATGTGCATTGAATATGGAGAAACTGCTTGAGACAAGAATTAATGAAGCTATAGAGCTACGTAAGAGTTTGGGGCTTCCATCAGCTAGTACAAATGCATTTCGTCTTGTCAATAGTGAAGGAGACAGATTATCAGGCCTAATAATTGACATATTTGGAGATGTAGCAGTAATAGCATCATCTGCCGCTTGGGTTGAGAAGTACAAAGCAGAAATAGAGGCTTGTGTTGGTAGAATCAATGAAATTAATCACATCAACTGGAGACCGTCTGTGGAAATTTTAAAAGAAGAAGGATTGGATGTGTCAAATTTGAAGGAAATGGATCCATCTATTTGCCCTCAGAGAACAAAGGTTATGGAAAACGGGATTTTTTATGCCATTTCACTTGAAGGGCAGAAGACAGGGTTCTATGCTGATCAGCGTGAAAACCGCAAATTCATATCAACAATTTCAGACAATCAGAAAGTTCTTGATATCTGCTGCTATAGTGGTGGTTTTGCGCTAAATGCTGCACGAGGTGGTGCTGTGAATGTCACAGGTGTTGACTCATCCTTGGGTGCTATCGAACTAGCTAAAGAAAATATTGCTCTTAACAACATGGATCCAGGAATAATATCATTTGTGAAAGAAGATGCAAGTGAGTTTATGAAGGGTGCTCTTTCTAGGAATGAATCATTTGATATTGTCATCTTAGATCCTCCTAAACTAGCACCACGAAAGAAGGTTTTACAAAATGCATCAGGCATGTATAGAAATTTAAATTCATTAGCAATGCAATTGACAAGGAGAGGTGGCCTTCTCATGACTTGTTCGTGTTCAGGAGCGATGACCCAAAGTGGGACGTTCTTGCGCATTCTTCAGAGTGCAGCATCAGCAGCAGGTAGAAAAATAACCGTTCTCAGGGATGCTGGAGCAGCATGTGACCATACCATTGACCCATCCTACCCAGAAGGCAAATACCTTTCCAACATTCTCCTGAGGGTGCTTTGA

Protein Analysis

727

Amino Acids

78.83

Weight (kDa)

8.76

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 325 - 388 8.2e-10 PUA-like domain
Methyltrans_SAM PF10672 515 - 675 1.9e-15 S-adenosylmethionine-dependent methyltransferase
TRM5-TYW2_MTfase PF02475 516 - 633 5.2e-06 TRM5/TYW2 methyltransferase domain
MTS PF05175 546 - 643 9.3e-08 Methyltransferase small domain
PrmA PF06325 548 - 628 1.2e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 549 - 677 2.4e-10 Methyltransferase domain
Cons_hypoth95 PF03602 550 - 632 2.4e-08 Conserved hypothetical protein 95
Methyltransf_25 PF13649 554 - 638 3.3e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 658, 2045
Acc36I ACCTGC 1 cut(s) 2063
AccB1I GGYRCC 1 cut(s) 763
AccB7I CCANNNNNTGG 2 cut(s) 582, 1073
AccI GTMKAC 1 cut(s) 896
AccII CGCG 2 cut(s) 204, 469
AclI AACGTT 1 cut(s) 391
AclWI GGATC 7 cut(s) 311, 434, 1511, 1524, 1790, 1803, 1883
AcsI RAATTY 5 cut(s) 1470, 1501, 1628, 1948, 1954
AcuI CTGAAG 1 cut(s) 2036
AcyI GRCGYC 1 cut(s) 873
AdeI CACNNNGTG 2 cut(s) 554, 1712
AfaI GTAC 4 cut(s) 72, 978, 1285, 1397
AfiI CCNNNNNNNGG 9 cut(s) 62, 86, 460, 582, 676, 853, 1073, 2031, 2094
AgsI TTSAA 5 cut(s) 935, 1006, 1207, 1507, 1583
AjnI CCWGG 5 cut(s) 55, 575, 743, 777, 1798
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 8 cut(s) 35, 473, 1094, 1168, 1244, 1252, 1280, 1766
AluI AGCT 8 cut(s) 35, 473, 1094, 1168, 1244, 1252, 1280, 1766
Alw21I GWGCWC 3 cut(s) 139, 334, 1852
Alw26I GTCTC 9 cut(s) 97, 261, 509, 894, 1051, 1144, 1220, 1311, 1450
AlwI GGATC 7 cut(s) 311, 434, 1511, 1524, 1790, 1803, 1883
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 5 cut(s) 166, 741, 774, 1330, 1987
ApaI GGGCCC 1 cut(s) 778
ApeKI GCWGC 9 cut(s) 182, 302, 701, 1165, 1674, 1703, 2060, 2069, 2108
ApoI RAATTY 5 cut(s) 1470, 1501, 1628, 1948, 1954
AseI ATTAAT 2 cut(s) 1236, 1440
Asp700I GAANNNNTTC 1 cut(s) 638
AspA2I CCTAGG 2 cut(s) 563, 725
AspLEI GCGC 3 cut(s) 659, 1696, 2046
AspS9I GGNCC 4 cut(s) 464, 741, 774, 775
AsuC2I CCSGG 2 cut(s) 838, 854
AsuHPI GGTGA 9 cut(s) 69, 71, 90, 244, 274, 419, 620, 643, 944
AvaII GGWCC 1 cut(s) 464
AvrII CCTAGG 2 cut(s) 563, 725
AxyI CCTNAGG 1 cut(s) 2171
BaeGI GKGCMC 1 cut(s) 778
BamHI GGATCC 2 cut(s) 1516, 1795
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 778
BauI CACGAG 1 cut(s) 1707
BbsI GAAGAC 4 cut(s) 535, 876, 998, 1598
Bbv12I GWGCWC 3 cut(s) 139, 334, 1852
BbvI GCAGC 9 cut(s) 169, 289, 688, 1177, 1661, 1690, 2072, 2081, 2120
BccI CCATC 7 cut(s) 245, 259, 1004, 1091, 1282, 1528, 2140
BceAI ACGGC 2 cut(s) 79, 142
BciT130I CCWGG 5 cut(s) 57, 577, 745, 779, 1800
BclI TGATCA 1 cut(s) 1609
BcnI CCSGG 2 cut(s) 838, 854
BcoDI GTCTC 9 cut(s) 97, 261, 509, 894, 1051, 1144, 1220, 1311, 1450
BfaI CTAG 9 cut(s) 47, 288, 564, 726, 1088, 1281, 1763, 1857, 1901
BfmI CTRYAG 3 cut(s) 156, 1245, 1678
BfuAI ACCTGC 1 cut(s) 2063
BlnI CCTAGG 2 cut(s) 563, 725
Bme1390I CCNGG 7 cut(s) 57, 577, 745, 779, 838, 854, 1800
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 4 cut(s) 464, 741, 774, 775
BmiI GGNNCC 5 cut(s) 465, 765, 776, 1518, 1797
BmrFI CCNGG 7 cut(s) 57, 577, 745, 779, 838, 854, 1800
BmsI GCATC 8 cut(s) 134, 330, 479, 1379, 1816, 1940, 2072, 2089
BpiI GAAGAC 4 cut(s) 535, 876, 998, 1598
BpmI CTGGAG 5 cut(s) 365, 640, 1095, 1474, 2124
BpuEI CTTGAG 2 cut(s) 965, 1244
BpuMI CCSGG 2 cut(s) 838, 854
BsaAI YACGTR 1 cut(s) 1256
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 873
BsaI GGTCTC 4 cut(s) 261, 509, 1051, 1450
BsaJI CCNNGG 7 cut(s) 118, 563, 576, 725, 777, 778, 1746
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 1068, 1098
Bsc4I CCNNNNNNNGG 9 cut(s) 62, 86, 460, 582, 676, 853, 1073, 2031, 2094
Bse1I ACTGG 4 cut(s) 477, 587, 1078, 1457
Bse21I CCTNAGG 1 cut(s) 2171
Bse3DI GCAATG 2 cut(s) 214, 1971
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 5 cut(s) 57, 577, 745, 779, 1800
BseDI CCNNGG 7 cut(s) 118, 563, 576, 725, 777, 778, 1746
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 9 cut(s) 62, 86, 460, 582, 676, 853, 1073, 2031, 2094
BseMI GCAATG 2 cut(s) 214, 1971
BseMII CTCAG 5 cut(s) 387, 618, 1547, 2107, 2162
BseNI ACTGG 4 cut(s) 477, 587, 1078, 1457
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 9 cut(s) 169, 289, 688, 1177, 1661, 1690, 2072, 2081, 2120
BsgI GTGCAG 2 cut(s) 1689, 2079
Bsh1236I CGCG 2 cut(s) 204, 469
BshFI GGCC 5 cut(s) 168, 743, 776, 1332, 1989
BshNI GGYRCC 1 cut(s) 763
BsiHKAI GWGCWC 3 cut(s) 139, 334, 1852
BsiSI CCGG 5 cut(s) 673, 788, 837, 854, 1156
BslFI GGGAC 3 cut(s) 593, 795, 2047
BslI CCNNNNNNNGG 9 cut(s) 62, 86, 460, 582, 676, 853, 1073, 2031, 2094
BsmAI GTCTC 9 cut(s) 97, 261, 509, 894, 1051, 1144, 1220, 1311, 1450
BsmFI GGGAC 3 cut(s) 593, 795, 2047
BsmI GAATGC 1 cut(s) 2046
BsnI GGCC 5 cut(s) 168, 743, 776, 1332, 1989
Bso31I GGTCTC 4 cut(s) 261, 509, 1051, 1450
Bsp120I GGGCCC 1 cut(s) 774
Bsp1286I GDGCHC 4 cut(s) 139, 334, 778, 1852
Bsp143I GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
BspANI GGCC 5 cut(s) 168, 743, 776, 1332, 1989
BspCNI CTCAG 5 cut(s) 386, 617, 1546, 2106, 2163
BspFNI CGCG 2 cut(s) 204, 469
BspHI TCATGA 1 cut(s) 1995
BspLI GGNNCC 5 cut(s) 465, 765, 776, 1518, 1797
BspMI ACCTGC 1 cut(s) 2063
BspPI GGATC 7 cut(s) 311, 434, 1511, 1524, 1790, 1803, 1883
BspT107I GGYRCC 1 cut(s) 763
BspTNI GGTCTC 4 cut(s) 261, 509, 1051, 1450
BsrDI GCAATG 2 cut(s) 214, 1971
BsrI ACTGG 4 cut(s) 477, 587, 1078, 1457
BssECI CCNNGG 7 cut(s) 118, 563, 576, 725, 777, 778, 1746
BssMI GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
BssNI GRCGYC 1 cut(s) 873
BssSI CACGAG 1 cut(s) 1707
BssT1I CCWWGG 4 cut(s) 118, 563, 725, 1746
Bst2BI CACGAG 1 cut(s) 1707
Bst2UI CCWGG 5 cut(s) 57, 577, 745, 779, 1800
Bst4CI ACNGT 4 cut(s) 628, 757, 1461, 2089
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 873
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstBAI YACGTR 1 cut(s) 1256
BstC8I GCNNGC 5 cut(s) 131, 135, 471, 706, 877
BstDEI CTNAG 8 cut(s) 373, 604, 713, 865, 1533, 1885, 2093, 2171
BstEII GGTNACC 1 cut(s) 280
BstFNI CGCG 2 cut(s) 204, 469
BstHHI GCGC 3 cut(s) 659, 1696, 2046
BstKTI GATC 9 cut(s) 237, 319, 325, 412, 429, 1519, 1612, 1798, 1891
BstMAI GTCTC 9 cut(s) 97, 261, 509, 894, 1051, 1144, 1220, 1311, 1450
BstMBI GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
BstNI CCWGG 5 cut(s) 57, 577, 745, 779, 1800
BstNSI RCATGY 3 cut(s) 1129, 1942, 2115
BstPI GGTNACC 1 cut(s) 280
BstSCI CCNGG 7 cut(s) 55, 575, 743, 777, 836, 852, 1798
BstSFI CTRYAG 3 cut(s) 156, 1245, 1678
BstSLI GKGCMC 1 cut(s) 778
BstSNI TACGTA 1 cut(s) 1256
BstUI CGCG 2 cut(s) 204, 469
BstV1I GCAGC 9 cut(s) 169, 289, 688, 1177, 1661, 1690, 2072, 2081, 2120
BstV2I GAAGAC 4 cut(s) 535, 876, 998, 1598
BstX2I RGATCY 5 cut(s) 316, 426, 1516, 1795, 1888
BstXI CCANNNNNNTGG 2 cut(s) 650, 1119
BstYI RGATCY 5 cut(s) 316, 426, 1516, 1795, 1888
Bsu36I CCTNAGG 1 cut(s) 2171
BsuRI GGCC 5 cut(s) 168, 743, 776, 1332, 1989
BtgZI GCGATG 1 cut(s) 2033
BtsIMutI CAGTG 2 cut(s) 484, 633
BveI ACCTGC 1 cut(s) 2063
Cac8I GCNNGC 5 cut(s) 131, 135, 471, 706, 877
CaiI CAGNNNCTG 1 cut(s) 582
CciI TCATGA 1 cut(s) 1995
CfoI GCGC 3 cut(s) 659, 1696, 2046
Cfr13I GGNCC 4 cut(s) 464, 741, 774, 775
CseI GACGC 1 cut(s) 881
Csp6I GTAC 4 cut(s) 71, 977, 1284, 1396
CviAII CATG 9 cut(s) 521, 915, 1126, 1144, 1197, 1792, 1939, 1996, 2112
CviQI GTAC 4 cut(s) 71, 977, 1284, 1396
DdeI CTNAG 8 cut(s) 373, 604, 713, 865, 1533, 1885, 2093, 2171
DpnI GATC 9 cut(s) 236, 318, 324, 411, 428, 1518, 1611, 1797, 1890
DpnII GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
DraI TTTAAA 2 cut(s) 1476, 1953
DraIII CACNNNGTG 2 cut(s) 554, 1712
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco105I TACGTA 1 cut(s) 1256
Eco130I CCWWGG 4 cut(s) 118, 563, 725, 1746
Eco147I AGGCCT 1 cut(s) 1332
Eco24I GRGCYC 1 cut(s) 778
Eco31I GGTCTC 4 cut(s) 261, 509, 1051, 1450
Eco32I GATATC 1 cut(s) 1669
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 2036
Eco81I CCTNAGG 1 cut(s) 2171
Eco91I GGTNACC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 775
EcoO65I GGTNACC 1 cut(s) 280
EcoRII CCWGG 5 cut(s) 55, 575, 743, 777, 1798
EcoRV GATATC 1 cut(s) 1669
EcoT14I CCWWGG 4 cut(s) 118, 563, 725, 1746
EcoT22I ATGCAT 2 cut(s) 1294, 1933
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 4 cut(s) 118, 563, 725, 1746
FaeI CATG 9 cut(s) 524, 918, 1129, 1147, 1200, 1795, 1942, 1999, 2115
FaqI GGGAC 3 cut(s) 593, 795, 2047
FatI CATG 9 cut(s) 520, 914, 1125, 1143, 1196, 1791, 1938, 1995, 2111
FauI CCCGC 3 cut(s) 122, 669, 884
FbaI TGATCA 1 cut(s) 1609
FblI GTMKAC 1 cut(s) 896
FriOI GRGCYC 1 cut(s) 778
FspBI CTAG 9 cut(s) 47, 288, 564, 726, 1088, 1281, 1763, 1857, 1901
FspI TGCGCA 2 cut(s) 658, 2045
GlaI GCGC 3 cut(s) 658, 1695, 2045
GsuI CTGGAG 5 cut(s) 365, 640, 1095, 1474, 2124
HaeIII GGCC 5 cut(s) 168, 743, 776, 1332, 1989
HapII CCGG 5 cut(s) 673, 788, 837, 854, 1156
HgaI GACGC 1 cut(s) 881
HhaI GCGC 3 cut(s) 659, 1696, 2046
Hin1I GRCGYC 1 cut(s) 873
Hin1II CATG 9 cut(s) 524, 918, 1129, 1147, 1200, 1795, 1942, 1999, 2115
Hin6I GCGC 3 cut(s) 657, 1694, 2044
HinP1I GCGC 3 cut(s) 657, 1694, 2044
HincII GTYRAC 1 cut(s) 1738
HindII GTYRAC 1 cut(s) 1738
HinfI GANTC 8 cut(s) 4, 90, 458, 615, 809, 1428, 1739, 1865
HpaII CCGG 5 cut(s) 673, 788, 837, 854, 1156
HphI GGTGA 9 cut(s) 69, 71, 90, 244, 274, 419, 620, 643, 944
Hpy166II GTNNAC 2 cut(s) 897, 1738
Hpy188I TCNGA 9 cut(s) 327, 376, 416, 442, 814, 1536, 1648, 1656, 2055
Hpy188III TCNNGA 9 cut(s) 248, 320, 619, 935, 982, 1664, 1996, 2013, 2170
Hpy8I GTNNAC 2 cut(s) 897, 1738
Hpy99I CGWCG 3 cut(s) 144, 439, 442
HpyCH4III ACNGT 4 cut(s) 628, 757, 1461, 2089
HpyCH4IV ACGT 3 cut(s) 391, 1255, 2036
HpyF3I CTNAG 8 cut(s) 373, 604, 713, 865, 1533, 1885, 2093, 2171
HpySE526I ACGT 3 cut(s) 391, 1255, 2036
Hsp92I GRCGYC 1 cut(s) 873
Hsp92II CATG 9 cut(s) 524, 918, 1129, 1147, 1200, 1795, 1942, 1999, 2115
HspAI GCGC 3 cut(s) 657, 1694, 2044
Ksp22I TGATCA 1 cut(s) 1609
Kzo9I GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
LmnI GCTCC 4 cut(s) 1034, 1100, 2015, 2105
Lsp1109I GCAGC 9 cut(s) 169, 289, 688, 1177, 1661, 1690, 2072, 2081, 2120
LweI GCATC 8 cut(s) 134, 330, 479, 1379, 1816, 1940, 2072, 2089
MaeI CTAG 9 cut(s) 47, 288, 564, 726, 1088, 1281, 1763, 1857, 1901
MaeII ACGT 3 cut(s) 391, 1255, 2036
MaeIII GTNAC 7 cut(s) 59, 122, 280, 608, 751, 1726, 2114
MalI GATC 9 cut(s) 236, 318, 324, 411, 428, 1518, 1611, 1797, 1890
MboI GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
MboII GAAGA 9 cut(s) 87, 398, 535, 881, 1003, 1493, 1603, 1835, 2042
MfeI CAATTG 1 cut(s) 1970
MflI RGATCY 5 cut(s) 316, 426, 1516, 1795, 1888
MhlI GDGCHC 4 cut(s) 139, 334, 778, 1852
MlyI GAGTC 2 cut(s) 99, 1733
MmeI TCCRAC 3 cut(s) 420, 837, 2184
Mph1103I ATGCAT 2 cut(s) 1294, 1933
MroXI GAANNNNTTC 1 cut(s) 638
MseI TTAA 5 cut(s) 1236, 1440, 1475, 1785, 1952
MslI CAYNNNNRTG 2 cut(s) 249, 497
MspA1I CMGCKG 1 cut(s) 1168
MspI CCGG 5 cut(s) 673, 788, 837, 854, 1156
MspR9I CCNGG 7 cut(s) 57, 577, 745, 779, 838, 854, 1800
MunI CAATTG 1 cut(s) 1970
Mva1269I GAATGC 1 cut(s) 2046
MvaI CCWGG 5 cut(s) 57, 577, 745, 779, 1800
MvnI CGCG 2 cut(s) 204, 469
NciI CCSGG 2 cut(s) 838, 854
NdeII GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
NlaIII CATG 9 cut(s) 524, 918, 1129, 1147, 1200, 1795, 1942, 1999, 2115
NlaIV GGNNCC 5 cut(s) 465, 765, 776, 1518, 1797
NmuCI GTSAC 6 cut(s) 59, 280, 608, 751, 1726, 2114
NsbI TGCGCA 2 cut(s) 658, 2045
NsiI ATGCAT 2 cut(s) 1294, 1933
NspI RCATGY 3 cut(s) 1129, 1942, 2115
OliI CACNNNNGTG 1 cut(s) 497
PagI TCATGA 1 cut(s) 1995
PasI CCCWGGG 1 cut(s) 778
PceI AGGCCT 1 cut(s) 1332
PctI GAATGC 1 cut(s) 2046
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 6 cut(s) 4, 458, 615, 809, 1428, 1865
PflMI CCANNNNNTGG 2 cut(s) 582, 1073
PfoI TCCNGGA 1 cut(s) 1798
PleI GAGTC 2 cut(s) 98, 1733
PpsI GAGTC 2 cut(s) 98, 1733
Ppu21I YACGTR 1 cut(s) 1256
PshBI ATTAAT 2 cut(s) 1236, 1440
Psp1406I AACGTT 1 cut(s) 391
Psp6I CCWGG 5 cut(s) 55, 575, 743, 777, 1798
PspEI GGTNACC 1 cut(s) 280
PspGI CCWGG 5 cut(s) 55, 575, 743, 777, 1798
PspN4I GGNNCC 5 cut(s) 465, 765, 776, 1518, 1797
PspOMI GGGCCC 1 cut(s) 774
PspPI GGNCC 4 cut(s) 464, 741, 774, 775
PstNI CAGNNNCTG 1 cut(s) 582
PsuI RGATCY 5 cut(s) 316, 426, 1516, 1795, 1888
PvuII CAGCTG 1 cut(s) 1168
RsaI GTAC 4 cut(s) 72, 978, 1285, 1397
RsaNI GTAC 4 cut(s) 71, 977, 1284, 1396
RseI CAYNNNNRTG 2 cut(s) 249, 497
SaqAI TTAA 5 cut(s) 1236, 1440, 1475, 1785, 1952
Sau3AI GATC 9 cut(s) 234, 316, 322, 409, 426, 1516, 1609, 1795, 1888
Sau96I GGNCC 4 cut(s) 464, 741, 774, 775
SchI GAGTC 2 cut(s) 99, 1733
ScrFI CCNGG 7 cut(s) 57, 577, 745, 779, 838, 854, 1800
SduI GDGCHC 4 cut(s) 139, 334, 778, 1852
SfaNI GCATC 8 cut(s) 134, 330, 479, 1379, 1816, 1940, 2072, 2089
SfcI CTRYAG 3 cut(s) 156, 1245, 1678
SinI GGWCC 1 cut(s) 464
SmiI ATTTAAAT 1 cut(s) 1953
SmiMI CAYNNNNRTG 2 cut(s) 249, 497
SmlI CTYRAG 2 cut(s) 980, 1223
SmoI CTYRAG 2 cut(s) 980, 1223
SnaBI TACGTA 1 cut(s) 1256
SseBI AGGCCT 1 cut(s) 1332
SspI AATATT 1 cut(s) 1777
SspMI CTAG 9 cut(s) 47, 288, 564, 726, 1088, 1281, 1763, 1857, 1901
StuI AGGCCT 1 cut(s) 1332
StyD4I CCNGG 7 cut(s) 55, 575, 743, 777, 836, 852, 1798
StyI CCWWGG 4 cut(s) 118, 563, 725, 1746
SwaI ATTTAAAT 1 cut(s) 1953
TaaI ACNGT 4 cut(s) 628, 757, 1461, 2089
TaiI ACGT 3 cut(s) 394, 1258, 2039
TaqI TCGA 2 cut(s) 139, 1758
TatI WGTACW 4 cut(s) 70, 976, 1283, 1395
TauI GCSGC 7 cut(s) 43, 46, 207, 384, 693, 696, 1382
TfiI GAWTC 6 cut(s) 4, 458, 615, 809, 1428, 1865
Tru1I TTAA 5 cut(s) 1236, 1440, 1475, 1785, 1952
Tru9I TTAA 5 cut(s) 1236, 1440, 1475, 1785, 1952
TscAI CASTG 2 cut(s) 484, 633
TseFI GTSAC 6 cut(s) 59, 280, 608, 751, 1726, 2114
TseI GCWGC 9 cut(s) 182, 302, 701, 1165, 1674, 1703, 2060, 2069, 2108
Tsp45I GTSAC 6 cut(s) 59, 280, 608, 751, 1726, 2114
TspDTI ATGAA 8 cut(s) 822, 1185, 1254, 1449, 1621, 1856, 1878, 1947
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 2 cut(s) 484, 633
Van91I CCANNNNNTGG 2 cut(s) 582, 1073
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 2 cut(s) 1236, 1440
XapI RAATTY 5 cut(s) 1470, 1501, 1628, 1948, 1954
XceI RCATGY 3 cut(s) 1129, 1942, 2115
XcmI CCANNNNNNNNNTGG 1 cut(s) 824
XmaJI CCTAGG 2 cut(s) 563, 725
XmiI GTMKAC 1 cut(s) 896
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 9 cut(s) 47, 288, 564, 726, 1088, 1281, 1763, 1857, 1901
Zsp2I ATGCAT 2 cut(s) 1294, 1933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.