pycom16g03430

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001447
Physical Location & Seq
Reverse (-)
2111402 .. 2113215
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGAAGGTACTAAATCAAAACCCTTTAAACTTCTTCTCAAAGCTCTTCAATTTCCAGCTCAACCTCCTCAATCTCCTCTTCTACTTCCTCCTCTTTGGCGGCGGTCTGACCCTCGGAATGGTGCTCAGCTTCTACATGAAAGACTTTTCCTTCAGTCTGCAATTAGCACAATTCTCCTTCTCGACCTCAACATCTGCATCCAATCCGCACAACCTAATGCCGAACGTAAAAGCAGTCCAACCGAACGAGAGTTATCGTCCTCCTCGTATAGGATTGAAGGAGTATCTAAAGCCGCCGGAGGTTTTCCACGACATGGACGATGTGGAGTTGCTGTGGAGAGCTTCGATGAGTCCTCGGATTTCCGAGTATCCGTTTCCCCGTGTTCCCAAGGTTGCTTTCTTGTTCTTGGTAAAGGGTCCTGTTCTTTTGGCTCCGTTGTGGGAGAAGTTCTTCAAGGGACATCAAGGCTTGTACTCGATTTATGTGCACTCGAATCCATCTTACAATGGATCGCACCCGGAAACTCCAGTTTTTCATGGCAGAAGAATTCCTAGCCAGGAAGTAGAATGGGGAAATGTGAACATGATTGAGGCCGAGCGCCGCCTGTTGGCGAATGCTCTTCTCGACATCTCAAACGAACGGTTCGTTCTGCTCTCAGAGTCATGCATTCCCCTCCACAACTTCTCCACTGTCTACAATTACGTCATCCGCTCCAACGAAACTTTTGTGCAAGTCTATGATGATCCAAGCTCGGTAGGGCGTGGCAGGTACAGTTTTAATATGTATCCACAAGTCTGGTTGAATCAATGGCGAAAAGGGTCGCAATGGTTTGAAATGGACAGAAGCCTTGCCATTGAAGTTGTCTCGGATAGAAAATACTTCCCGGTGTTCCAAAGTTACTGCAAGGGATCATGCTACGCAGACGAGCATTACTTACCAACATTTGTGAACATGAAATTTGGGAAGAAGAATTCGAATAGGAGTTTGACCTGGGTTGACTGGTCGAGGGGCGGCCCACACCCTGCCAAGTTTACTAGAACAGATGTGACGGTTGATGTTTTGAATAGCTTGAGGAATGGGACTTCTTGTATATACAATGGAAGAGTATCCAACGTTTGTTACTTGTTTGCTAGAAAGTTTGGGCCTGAAACACTAGAGAGACTGCTGAAGTTTGCGCCGAAGCTCATGGGATTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

401

Amino Acids

46.36

Weight (kDa)

9.37

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 131 - 357 1.5e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 756
AccB7I CCANNNNNTGG 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 711
AccI GTMKAC 1 cut(s) 693
AciI CCGC 7 cut(s) 99, 102, 206, 293, 601, 709, 1011
AclI AACGTT 1 cut(s) 1113
AclWI GGATC 3 cut(s) 517, 737, 916
AcsI RAATTY 3 cut(s) 546, 956, 970
AcuI CTGAAG 2 cut(s) 136, 1187
AfaI GTAC 3 cut(s) 9, 473, 770
AfiI CCNNNNNNNGG 4 cut(s) 118, 269, 313, 607
AgsI TTSAA 8 cut(s) 49, 277, 454, 802, 833, 857, 1063, 1195
AjnI CCWGG 2 cut(s) 555, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 7 cut(s) 43, 58, 129, 341, 750, 1068, 1183
AluI AGCT 7 cut(s) 43, 58, 129, 341, 750, 1068, 1183
Alw21I GWGCWC 2 cut(s) 126, 489
Alw26I GTCTC 2 cut(s) 868, 1153
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 3 cut(s) 517, 737, 916
AoxI GGCC 3 cut(s) 591, 1012, 1142
ApaLI GTGCAC 1 cut(s) 485
ApoI RAATTY 3 cut(s) 546, 956, 970
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 2 cut(s) 600, 1177
AspS9I GGNCC 3 cut(s) 416, 1013, 1142
AsuC2I CCSGG 2 cut(s) 518, 884
AsuII TTCGAA 1 cut(s) 974
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 126, 489
BccI CCATC 1 cut(s) 505
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BciT130I CCWGG 2 cut(s) 557, 991
BciVI GTATCC 3 cut(s) 378, 795, 1117
BcnI CCSGG 2 cut(s) 518, 884
BcoDI GTCTC 2 cut(s) 868, 1153
BfaI CTAG 4 cut(s) 552, 1035, 1131, 1154
BfoI RGCGCY 1 cut(s) 601
BfuAI ACCTGC 1 cut(s) 756
BfuI GTATCC 3 cut(s) 378, 795, 1117
BisI GCNGC 4 cut(s) 100, 293, 601, 1012
BlpI GCTNAGC 1 cut(s) 125
BlsI GCNGC 4 cut(s) 101, 294, 602, 1013
Bme1390I CCNGG 4 cut(s) 518, 557, 884, 991
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 416, 1013, 1142
BmiI GGNNCC 2 cut(s) 417, 432
BmrFI CCNGG 4 cut(s) 518, 557, 884, 991
BmsI GCATC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 974
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 2 cut(s) 518, 884
BsaJI CCNNGG 4 cut(s) 112, 353, 387, 990
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 668, 698
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 269, 313, 607
Bse1I ACTGG 2 cut(s) 527, 1004
Bse3DI GCAATG 1 cut(s) 830
BseBI CCWGG 2 cut(s) 557, 991
BseDI CCNNGG 4 cut(s) 112, 353, 387, 990
BseGI GGATG 2 cut(s) 197, 705
BseLI CCNNNNNNNGG 4 cut(s) 118, 269, 313, 607
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 2 cut(s) 139, 669
BseNI ACTGG 2 cut(s) 527, 1004
BseRI GAGGAG 4 cut(s) 56, 65, 80, 252
BseSI GKGCMC 1 cut(s) 489
BshFI GGCC 3 cut(s) 593, 1014, 1144
BsiHKAI GWGCWC 2 cut(s) 126, 489
BsiSI CCGG 3 cut(s) 296, 518, 884
BslFI GGGAC 2 cut(s) 471, 1093
BslI CCNNNNNNNGG 4 cut(s) 118, 269, 313, 607
BsmAI GTCTC 2 cut(s) 868, 1153
BsmFI GGGAC 2 cut(s) 471, 1093
BsmI GAATGC 2 cut(s) 619, 666
BsnI GGCC 3 cut(s) 593, 1014, 1144
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 2 cut(s) 126, 489
Bsp143I GATC 3 cut(s) 509, 742, 908
Bsp1720I GCTNAGC 1 cut(s) 125
BspACI CCGC 7 cut(s) 99, 102, 206, 293, 601, 709, 1011
BspANI GGCC 3 cut(s) 593, 1014, 1144
BspCNI CTCAG 2 cut(s) 138, 668
BspLI GGNNCC 2 cut(s) 417, 432
BspMI ACCTGC 1 cut(s) 756
BspPI GGATC 3 cut(s) 517, 737, 916
BspQI GCTCTTC 2 cut(s) 50, 624
BspT104I TTCGAA 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 711
BsrDI GCAATG 1 cut(s) 830
BsrI ACTGG 2 cut(s) 527, 1004
BssECI CCNNGG 4 cut(s) 112, 353, 387, 990
BssMI GATC 3 cut(s) 509, 742, 908
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 557, 991
Bst4CI ACNGT 4 cut(s) 642, 691, 773, 1051
Bst6I CTCTTC 4 cut(s) 50, 83, 624, 1096
BstBI TTCGAA 1 cut(s) 974
BstDEI CTNAG 2 cut(s) 125, 655
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 197, 705
BstH2I RGCGCY 1 cut(s) 601
BstHHI GCGC 2 cut(s) 600, 1177
BstKTI GATC 3 cut(s) 512, 745, 911
BstMAI GTCTC 2 cut(s) 868, 1153
BstMBI GATC 3 cut(s) 509, 742, 908
BstNI CCWGG 2 cut(s) 557, 991
BstSCI CCNGG 4 cut(s) 516, 555, 882, 989
BstSLI GKGCMC 1 cut(s) 489
BstXI CCANNNNNNTGG 1 cut(s) 795
BsuI GTATCC 3 cut(s) 378, 795, 1117
BsuRI GGCC 3 cut(s) 593, 1014, 1144
BtsCI GGATG 2 cut(s) 197, 705
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 1 cut(s) 756
CfoI GCGC 2 cut(s) 600, 1177
Cfr13I GGNCC 3 cut(s) 416, 1013, 1142
Csp6I GTAC 3 cut(s) 8, 472, 769
CviAII CATG 8 cut(s) 136, 313, 536, 583, 663, 912, 952, 1186
CviQI GTAC 3 cut(s) 8, 472, 769
DdeI CTNAG 2 cut(s) 125, 655
DpnI GATC 3 cut(s) 511, 744, 910
DpnII GATC 3 cut(s) 509, 742, 908
DraI TTTAAA 1 cut(s) 27
Eam1104I CTCTTC 4 cut(s) 50, 83, 624, 1096
EarI CTCTTC 4 cut(s) 50, 83, 624, 1096
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 136, 1187
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 416
EcoRI GAATTC 2 cut(s) 546, 970
EcoRII CCWGG 2 cut(s) 555, 989
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 668
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 8 cut(s) 139, 316, 539, 586, 666, 915, 955, 1189
FaqI GGGAC 2 cut(s) 471, 1093
FatI CATG 8 cut(s) 135, 312, 535, 582, 662, 911, 951, 1185
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 4 cut(s) 100, 293, 601, 1012
FokI GGATG 2 cut(s) 184, 692
Fsp4HI GCNGC 4 cut(s) 100, 293, 601, 1012
FspBI CTAG 4 cut(s) 552, 1035, 1131, 1154
GlaI GCGC 2 cut(s) 599, 1176
GluI GCNGC 4 cut(s) 100, 293, 601, 1012
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 1 cut(s) 601
HaeIII GGCC 3 cut(s) 593, 1014, 1144
HapII CCGG 3 cut(s) 296, 518, 884
HhaI GCGC 2 cut(s) 600, 1177
Hin1II CATG 8 cut(s) 139, 316, 539, 586, 666, 915, 955, 1189
Hin6I GCGC 2 cut(s) 598, 1175
HinP1I GCGC 2 cut(s) 598, 1175
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HinfI GANTC 5 cut(s) 349, 493, 659, 802, 1191
HpaII CCGG 3 cut(s) 296, 518, 884
Hpy166II GTNNAC 6 cut(s) 487, 580, 694, 949, 997, 1032
Hpy188I TCNGA 6 cut(s) 108, 116, 357, 364, 658, 868
Hpy188III TCNNGA 2 cut(s) 181, 623
Hpy8I GTNNAC 6 cut(s) 487, 580, 694, 949, 997, 1032
HpyAV CCTTC 3 cut(s) 160, 187, 271
HpyCH4III ACNGT 4 cut(s) 642, 691, 773, 1051
HpyCH4IV ACGT 3 cut(s) 225, 702, 1113
HpyCH4V TGCA 6 cut(s) 160, 197, 487, 666, 730, 903
HpyF3I CTNAG 2 cut(s) 125, 655
HpySE526I ACGT 3 cut(s) 225, 702, 1113
Hsp92II CATG 8 cut(s) 139, 316, 539, 586, 666, 915, 955, 1189
HspAI GCGC 2 cut(s) 598, 1175
Kzo9I GATC 3 cut(s) 509, 742, 908
LguI GCTCTTC 2 cut(s) 50, 624
LmnI GCTCC 2 cut(s) 436, 716
LweI GCATC 1 cut(s) 206
MaeI CTAG 4 cut(s) 552, 1035, 1131, 1154
MaeII ACGT 3 cut(s) 225, 702, 1113
MaeIII GTNAC 3 cut(s) 896, 1045, 1118
MalI GATC 3 cut(s) 511, 744, 910
MbiI CCGCTC 1 cut(s) 711
MboI GATC 3 cut(s) 509, 742, 908
MboII GAAGA 9 cut(s) 25, 37, 70, 442, 555, 611, 976, 979, 1113
MhlI GDGCHC 2 cut(s) 126, 489
MluCI AATT 7 cut(s) 49, 161, 170, 546, 697, 956, 970
MlyI GAGTC 2 cut(s) 358, 668
MmeI TCCRAC 3 cut(s) 262, 738, 1134
Mph1103I ATGCAT 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 2 cut(s) 26, 777
MspI CCGG 3 cut(s) 296, 518, 884
MspR9I CCNGG 4 cut(s) 518, 557, 884, 991
Mva1269I GAATGC 2 cut(s) 619, 666
MvaI CCWGG 2 cut(s) 557, 991
NciI CCSGG 2 cut(s) 518, 884
NdeII GATC 3 cut(s) 509, 742, 908
NlaIII CATG 8 cut(s) 139, 316, 539, 586, 666, 915, 955, 1189
NlaIV GGNNCC 2 cut(s) 417, 432
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 1 cut(s) 1045
NsiI ATGCAT 1 cut(s) 668
NspV TTCGAA 1 cut(s) 974
PciSI GCTCTTC 2 cut(s) 50, 624
PcsI WCGNNNNNNNCGW 3 cut(s) 262, 315, 642
PctI GAATGC 2 cut(s) 619, 666
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 3 cut(s) 493, 802, 1191
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 4 cut(s) 101, 294, 602, 1013
PleI GAGTC 2 cut(s) 357, 667
PpsI GAGTC 2 cut(s) 357, 667
PpuMI RGGWCCY 1 cut(s) 416
Psp1406I AACGTT 1 cut(s) 1113
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 555, 989
PspGI CCWGG 2 cut(s) 555, 989
PspN4I GGNNCC 2 cut(s) 417, 432
PspPI GGNCC 3 cut(s) 416, 1013, 1142
PspPPI RGGWCCY 1 cut(s) 416
RsaI GTAC 3 cut(s) 9, 473, 770
RsaNI GTAC 3 cut(s) 8, 472, 769
SapI GCTCTTC 2 cut(s) 50, 624
SaqAI TTAA 2 cut(s) 26, 777
SatI GCNGC 4 cut(s) 100, 293, 601, 1012
Sau3AI GATC 3 cut(s) 509, 742, 908
Sau96I GGNCC 3 cut(s) 416, 1013, 1142
SchI GAGTC 2 cut(s) 358, 668
ScrFI CCNGG 4 cut(s) 518, 557, 884, 991
SduI GDGCHC 2 cut(s) 126, 489
SfaNI GCATC 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 1069
SmoI CTYRAG 1 cut(s) 1069
Sse9I AATT 7 cut(s) 49, 161, 170, 546, 697, 956, 970
SsiI CCGC 7 cut(s) 99, 102, 206, 293, 601, 709, 1011
SspMI CTAG 4 cut(s) 552, 1035, 1131, 1154
StyD4I CCNGG 4 cut(s) 516, 555, 882, 989
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 4 cut(s) 642, 691, 773, 1051
TaiI ACGT 3 cut(s) 228, 705, 1116
TaqI TCGA 7 cut(s) 182, 344, 476, 491, 624, 974, 1004
TasI AATT 7 cut(s) 49, 161, 170, 546, 697, 956, 970
TatI WGTACW 1 cut(s) 471
TauI GCSGC 4 cut(s) 102, 295, 603, 1014
TfiI GAWTC 3 cut(s) 493, 802, 1191
Tru1I TTAA 2 cut(s) 26, 777
Tru9I TTAA 2 cut(s) 26, 777
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 1 cut(s) 1045
Tsp45I GTSAC 1 cut(s) 1045
TspDTI ATGAA 4 cut(s) 17, 152, 524, 968
TspGWI ACGGA 2 cut(s) 360, 423
TspRI CASTG 1 cut(s) 694
Van91I CCANNNNNTGG 1 cut(s) 313
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 416
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 3 cut(s) 546, 956, 970
XmiI GTMKAC 1 cut(s) 693
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 4 cut(s) 552, 1035, 1131, 1154
Zsp2I ATGCAT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.