pycom17g07830
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001688
Physical Location & Seq
Forward (+)
5790961 .. 5791683
723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGGAAATGCAGGAAGCAACAGAAGTTGAGGCAAGCCTAAGAGGCCAAGCAGATAAATGGAAGTACATGTTTGGTATTGCTGATTCCATGGCCTTAAAAATTGCTGGTGCCTCAAACTCAAAGCCTATGATCACTTTGTCCCAATTAGCCTCATGCATAGCACCCTCCCCAGCAATCACATACCTCACACGCATCATGAGACTTCTTGTCCGCCGAAATATATTCACTGTTCATCACCCATCCGATGGCGGGGAACCTATCTATGGGTTGACACACTTATCAAGATGGTTGCTATATGATGATGAGCTGAGCCTAGCTCCAATGCTTGTGATGGAGAATCACCCTTGCCTAATGGCTCCTTGGCACTATTTCGGCCGATGCATCAAAGGAGGAGGTCCATGTGCCTTTAAGATGGCCCATGGGCTTGAGATTTGGGACTACGCGTCTCAAAATCCAGAGTTCAACAAATTATTCAATGGTGGCATGGCCTGTACGGCTAGGGGTTTCAATTTTGATCTGCCGCATGTCGTAGCCATGGCGCCGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

183

Amino Acids

20.27

Weight (kDa)

6.64

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 20 - 98 6.2e-09 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 107, 538
AccB7I CCANNNNNTGG 1 cut(s) 245
AccII CGCG 1 cut(s) 443
AciI CCGC 3 cut(s) 211, 249, 521
AcoI YGGCCR 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 2 cut(s) 65, 493
AfiI CCNNNNNNNGG 3 cut(s) 245, 249, 263
AflIII ACRYGT 2 cut(s) 66, 441
AgsI TTSAA 3 cut(s) 463, 475, 508
AhdI GACNNNNNGTC 2 cut(s) 206, 442
AluBI AGCT 2 cut(s) 307, 317
AluI AGCT 2 cut(s) 307, 317
Alw26I GTCTC 2 cut(s) 193, 450
AoxI GGCC 5 cut(s) 43, 90, 373, 414, 486
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 2 cut(s) 395, 415
AsuHPI GGTGA 2 cut(s) 227, 332
AvaII GGWCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 107, 538
BccI CCATC 5 cut(s) 239, 247, 279, 325, 406
BceAI ACGGC 1 cut(s) 510
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 193, 450
BfaI CTAG 2 cut(s) 314, 498
BfoI RGCGCY 1 cut(s) 542
BglI GCCNNNNNGGC 2 cut(s) 42, 494
BisI GCNGC 1 cut(s) 521
BlpI GCTNAGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 522
Bme18I GGWCC 1 cut(s) 395
BmeRI GACNNNNNGTC 2 cut(s) 206, 442
BmgT120I GGNCC 2 cut(s) 395, 415
BmiI GGNNCC 4 cut(s) 109, 255, 357, 540
BmsI GCATC 3 cut(s) 201, 368, 390
BplI GAGNNNNNCTC 2 cut(s) 301, 333
Bpu1102I GCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 4 cut(s) 87, 359, 418, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 249, 263
BseDI CCNNGG 4 cut(s) 87, 359, 418, 534
BseGI GGATG 1 cut(s) 239
BseLI CCNNNNNNNGG 3 cut(s) 245, 249, 263
BseMII CTCAG 1 cut(s) 299
BseRI GAGGAG 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 373
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 443
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 5 cut(s) 45, 92, 375, 416, 488
BshNI GGYRCC 2 cut(s) 107, 538
BsiEI CGRYCG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 124, 449
BslI CCNNNNNNNGG 3 cut(s) 245, 249, 263
BsmAI GTCTC 2 cut(s) 193, 450
BsmBI CGTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 124, 449
BsnI GGCC 5 cut(s) 45, 92, 375, 416, 488
Bsp143I GATC 2 cut(s) 129, 514
Bsp1720I GCTNAGC 1 cut(s) 308
Bsp19I CCATGG 3 cut(s) 87, 418, 534
BspACI CCGC 3 cut(s) 211, 249, 521
BspANI GGCC 5 cut(s) 45, 92, 375, 416, 488
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 1 cut(s) 443
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 4 cut(s) 109, 255, 357, 540
BspT107I GGYRCC 2 cut(s) 107, 538
BssECI CCNNGG 4 cut(s) 87, 359, 418, 534
BssMI GATC 2 cut(s) 129, 514
BssNI GRCGYC 1 cut(s) 539
BssT1I CCWWGG 4 cut(s) 87, 359, 418, 534
Bst4CI ACNGT 1 cut(s) 229
BstACI GRCGYC 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 38, 308
BstDSI CCRYGG 3 cut(s) 87, 418, 534
BstF5I GGATG 1 cut(s) 239
BstFNI CGCG 1 cut(s) 443
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 2 cut(s) 132, 517
BstMAI GTCTC 2 cut(s) 193, 450
BstMBI GATC 2 cut(s) 129, 514
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 2 cut(s) 42, 494
BstNSI RCATGY 2 cut(s) 70, 527
BstUI CGCG 1 cut(s) 443
BstZI CGGCCG 1 cut(s) 373
BsuRI GGCC 5 cut(s) 45, 92, 375, 416, 488
BtgI CCRYGG 3 cut(s) 87, 418, 534
BtsCI GGATG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 34
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 2 cut(s) 395, 415
CseI GACGC 1 cut(s) 432
Csp6I GTAC 2 cut(s) 64, 492
CviAII CATG 9 cut(s) 67, 88, 153, 196, 399, 419, 484, 524, 535
CviQI GTAC 2 cut(s) 64, 492
DdeI CTNAG 2 cut(s) 38, 308
DinI GGCGCC 1 cut(s) 540
DpnI GATC 2 cut(s) 131, 516
DpnII GATC 2 cut(s) 129, 514
DriI GACNNNNNGTC 2 cut(s) 206, 442
EaeI YGGCCR 1 cut(s) 373
EagI CGGCCG 1 cut(s) 373
Eam1105I GACNNNNNGTC 2 cut(s) 206, 442
EciI GGCGGA 1 cut(s) 200
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 4 cut(s) 87, 359, 418, 534
Eco47I GGWCC 1 cut(s) 395
Eco52I CGGCCG 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 87, 359, 418, 534
EcoT22I ATGCAT 2 cut(s) 158, 383
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
ErhI CCWWGG 4 cut(s) 87, 359, 418, 534
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 9 cut(s) 70, 91, 156, 199, 402, 422, 487, 527, 538
FaqI GGGAC 2 cut(s) 124, 449
FatI CATG 9 cut(s) 66, 87, 152, 195, 398, 418, 483, 523, 534
FauI CCCGC 1 cut(s) 242
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 521
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 1 cut(s) 521
FspBI CTAG 2 cut(s) 314, 498
GlaI GCGC 1 cut(s) 540
GluI GCNGC 1 cut(s) 521
GsaI CCCAGC 1 cut(s) 173
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 5 cut(s) 45, 92, 375, 416, 488
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 9 cut(s) 70, 91, 156, 199, 402, 422, 487, 527, 538
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 2 cut(s) 83, 337
HphI GGTGA 2 cut(s) 227, 332
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 3 cut(s) 196, 282, 455
Hpy8I GTNNAC 1 cut(s) 270
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 10, 156, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 494
HpyF3I CTNAG 2 cut(s) 38, 308
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 9 cut(s) 70, 91, 156, 199, 402, 422, 487, 527, 538
HspAI GCGC 1 cut(s) 539
KasI GGCGCC 1 cut(s) 538
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 2 cut(s) 129, 514
LmnI GCTCC 2 cut(s) 322, 361
LpnPI CCDG 4 cut(s) 90, 183, 468, 502
LweI GCATC 3 cut(s) 201, 368, 390
MaeI CTAG 2 cut(s) 314, 498
MalI GATC 2 cut(s) 131, 516
MboI GATC 2 cut(s) 129, 514
MluCI AATT 4 cut(s) 99, 143, 467, 508
MluI ACGCGT 1 cut(s) 441
Mly113I GGCGCC 1 cut(s) 539
MnlI CCTC 8 cut(s) 22, 35, 121, 160, 175, 194, 383, 386
Mph1103I ATGCAT 2 cut(s) 158, 383
MseI TTAA 2 cut(s) 95, 408
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 2 cut(s) 42, 494
NarI GGCGCC 1 cut(s) 539
NcoI CCATGG 3 cut(s) 87, 418, 534
NdeII GATC 2 cut(s) 129, 514
NlaIII CATG 9 cut(s) 70, 91, 156, 199, 402, 422, 487, 527, 538
NlaIV GGNNCC 4 cut(s) 109, 255, 357, 540
NsiI ATGCAT 2 cut(s) 158, 383
NspI RCATGY 2 cut(s) 70, 527
PagI TCATGA 1 cut(s) 195
PciI ACATGT 1 cut(s) 66
PfeI GAWTC 2 cut(s) 83, 337
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 1 cut(s) 522
PluTI GGCGCC 1 cut(s) 542
PscI ACATGT 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 169
PspN4I GGNNCC 4 cut(s) 109, 255, 357, 540
PspPI GGNCC 2 cut(s) 395, 415
RsaI GTAC 2 cut(s) 65, 493
RsaNI GTAC 2 cut(s) 64, 492
SaqAI TTAA 2 cut(s) 95, 408
SatI GCNGC 1 cut(s) 521
Sau3AI GATC 2 cut(s) 129, 514
Sau96I GGNCC 2 cut(s) 395, 415
SetI ASST 5 cut(s) 186, 259, 309, 319, 397
SfaNI GCATC 3 cut(s) 201, 368, 390
SfoI GGCGCC 1 cut(s) 540
SinI GGWCC 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 4 cut(s) 99, 143, 467, 508
SsiI CCGC 3 cut(s) 211, 249, 521
SspDI GGCGCC 1 cut(s) 538
SspMI CTAG 2 cut(s) 314, 498
StyI CCWWGG 4 cut(s) 87, 359, 418, 534
TaaI ACNGT 1 cut(s) 229
TasI AATT 4 cut(s) 99, 143, 467, 508
TatI WGTACW 1 cut(s) 63
TauI GCSGC 1 cut(s) 523
TfiI GAWTC 2 cut(s) 83, 337
Tru1I TTAA 2 cut(s) 95, 408
Tru9I TTAA 2 cut(s) 95, 408
TscAI CASTG 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 395
XceI RCATGY 2 cut(s) 70, 527
XspI CTAG 2 cut(s) 314, 498
Zsp2I ATGCAT 2 cut(s) 158, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.