Rh2AG405100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001688
Physical Location & Seq
Reverse (-)
60375339 .. 60375875
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG405100.1

Sequence Viewer

Length: 537 bp
ATGGAAGCAGTTACAGAATTGGATGAAGCAACTCTAAGAGGCCAAGCAGATGTATGGAAGTACATGCTCGGCTTTGCAGATTCCATGGCTTTGAAATCTGTTGTGGAGCTCCGCATACCAGATATCATACACTCTCATGGTCATGCATTGACTTTGTCTGAAATAGCCTCATCCATCGATTTTGCATCACCCTCTCCAGACATCACATGCCTCTCTCGGATCATGCGGTTGCTGGTCCGCAGAAACATCTTCACCGCACATCATGGCGGCGGAGATTCCGAAGAAACCCTCTATGGGTTGACTCCTTCATCTAGATGGCTCTTGTATGACTCGGAGCTCACTCTCGCTCCAATGGTTCTCTCGGAGACAAATCCTATTCTAATGGCTCCAATGCACTATTTCAGCCAATGCATCAAGCAAGGTGGCCGATGTGCCTTTGAGAAAGCGCATGGGTGTGAAATTGTGCAATTCTTCTCCGAAAACCTTGAGATCAACCGATTGTTCAATGATGCCATGGCGTGTACCCTAAGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

178

Amino Acids

19.88

Weight (kDa)

5.28

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 19 - 108 3.2e-21 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 535
AciI CCGC 6 cut(s) 112, 226, 238, 255, 267, 270
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 424
AfaI GTAC 2 cut(s) 62, 523
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 2 cut(s) 94, 505
AluBI AGCT 2 cut(s) 109, 337
AluI AGCT 2 cut(s) 109, 337
Alw21I GWGCWC 2 cut(s) 111, 339
Alw26I GTCTC 1 cut(s) 359
AlwI GGATC 1 cut(s) 227
AoxI GGCC 2 cut(s) 40, 424
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 180, 244
AvaII GGWCC 1 cut(s) 235
BanII GRGCYC 2 cut(s) 111, 339
Bbv12I GWGCWC 2 cut(s) 111, 339
BccI CCATC 2 cut(s) 182, 309
BcoDI GTCTC 1 cut(s) 359
BfaI CTAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 387
BmsI GCATC 3 cut(s) 194, 420, 499
BpmI CTGGAG 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 84, 513
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 294
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 2 cut(s) 84, 513
BseGI GGATG 2 cut(s) 28, 170
BseLI CCNNNNNNNGG 1 cut(s) 294
BshFI GGCC 2 cut(s) 42, 426
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 111, 339
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 359
BsnI GGCC 2 cut(s) 42, 426
Bsp1286I GDGCHC 2 cut(s) 111, 339
Bsp143I GATC 2 cut(s) 219, 489
Bsp19I CCATGG 2 cut(s) 84, 513
BspACI CCGC 6 cut(s) 112, 226, 238, 255, 267, 270
BspANI GGCC 2 cut(s) 42, 426
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 387
BspPI GGATC 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 84, 513
BssMI GATC 2 cut(s) 219, 489
BssT1I CCWWGG 2 cut(s) 84, 513
BstDEI CTNAG 2 cut(s) 35, 527
BstDSI CCRYGG 2 cut(s) 84, 513
BstF5I GGATG 2 cut(s) 28, 170
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 2 cut(s) 222, 492
BstMAI GTCTC 1 cut(s) 359
BstMBI GATC 2 cut(s) 219, 489
BstNSI RCATGY 2 cut(s) 67, 210
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 2 cut(s) 42, 426
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 84, 513
BtsCI GGATG 2 cut(s) 28, 170
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 2 cut(s) 61, 522
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 9 cut(s) 64, 85, 137, 143, 207, 223, 263, 449, 514
CviQI GTAC 2 cut(s) 61, 522
DdeI CTNAG 2 cut(s) 35, 527
DpnI GATC 2 cut(s) 221, 491
DpnII GATC 2 cut(s) 219, 489
EaeI YGGCCR 1 cut(s) 424
EciI GGCGGA 1 cut(s) 285
Ecl136II GAGCTC 2 cut(s) 109, 337
Eco130I CCWWGG 2 cut(s) 84, 513
Eco24I GRGCYC 2 cut(s) 111, 339
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 2 cut(s) 109, 337
EcoICRI GAGCTC 2 cut(s) 109, 337
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 84, 513
EcoT22I ATGCAT 2 cut(s) 148, 413
EcoT38I GRGCYC 2 cut(s) 111, 339
ErhI CCWWGG 2 cut(s) 84, 513
FaeI CATG 9 cut(s) 67, 88, 140, 146, 210, 226, 266, 452, 517
FatI CATG 9 cut(s) 63, 84, 136, 142, 206, 222, 262, 448, 513
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 2 cut(s) 35, 157
FriOI GRGCYC 2 cut(s) 111, 339
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 1 cut(s) 312
GlaI GCGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 268
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 42, 426
HhaI GCGC 1 cut(s) 448
Hin1II CATG 9 cut(s) 67, 88, 140, 146, 210, 226, 266, 452, 517
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 4 cut(s) 80, 275, 301, 329
HphI GGTGA 2 cut(s) 180, 244
Hpy166II GTNNAC 2 cut(s) 300, 522
Hpy188I TCNGA 6 cut(s) 160, 219, 280, 334, 364, 478
Hpy188III TCNNGA 2 cut(s) 197, 312
Hpy8I GTNNAC 2 cut(s) 300, 522
HpyAV CCTTC 1 cut(s) 315
HpyCH4V TGCA 6 cut(s) 77, 146, 185, 394, 411, 466
HpyF3I CTNAG 2 cut(s) 35, 527
Hsp92II CATG 9 cut(s) 67, 88, 140, 146, 210, 226, 266, 452, 517
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 2 cut(s) 219, 489
LmnI GCTCC 5 cut(s) 106, 114, 334, 352, 391
LpnPI CCDG 3 cut(s) 132, 210, 218
LweI GCATC 3 cut(s) 194, 420, 499
MaeI CTAG 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 221, 491
MboI GATC 2 cut(s) 219, 489
MboII GAAGA 3 cut(s) 241, 293, 463
MhlI GDGCHC 2 cut(s) 111, 339
MluCI AATT 3 cut(s) 17, 459, 467
MlyI GAGTC 2 cut(s) 295, 323
MnlI CCTC 5 cut(s) 32, 178, 202, 221, 299
Mph1103I ATGCAT 2 cut(s) 148, 413
MslI CAYNNNNRTG 4 cut(s) 135, 141, 313, 453
NcoI CCATGG 2 cut(s) 84, 513
NdeII GATC 2 cut(s) 219, 489
NlaIII CATG 9 cut(s) 67, 88, 140, 146, 210, 226, 266, 452, 517
NlaIV GGNNCC 1 cut(s) 387
NmeAIII GCCGAG 1 cut(s) 48
NsiI ATGCAT 2 cut(s) 148, 413
NspI RCATGY 2 cut(s) 67, 210
PfeI GAWTC 2 cut(s) 80, 275
PflFI GACNNNGTC 1 cut(s) 154
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 2 cut(s) 295, 323
PpsI GAGTC 2 cut(s) 295, 323
PsiI TTATAA 1 cut(s) 535
Psp124BI GAGCTC 2 cut(s) 111, 339
PspN4I GGNNCC 1 cut(s) 387
PspPI GGNCC 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 154
RsaI GTAC 2 cut(s) 62, 523
RsaNI GTAC 2 cut(s) 61, 522
RseI CAYNNNNRTG 4 cut(s) 135, 141, 313, 453
SacI GAGCTC 2 cut(s) 111, 339
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 219, 489
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 295, 323
SduI GDGCHC 2 cut(s) 111, 339
SetI ASST 4 cut(s) 111, 339, 424, 486
SfaNI GCATC 3 cut(s) 194, 420, 499
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 4 cut(s) 135, 141, 313, 453
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 17, 459, 467
SsiI CCGC 6 cut(s) 112, 226, 238, 255, 267, 270
SspMI CTAG 1 cut(s) 312
SstI GAGCTC 2 cut(s) 111, 339
StyI CCWWGG 2 cut(s) 84, 513
TaqI TCGA 1 cut(s) 177
TasI AATT 3 cut(s) 17, 459, 467
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 2 cut(s) 80, 275
TspDTI ATGAA 2 cut(s) 39, 297
Tth111I GACNNNGTC 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 235
XbaI TCTAGA 1 cut(s) 311
XceI RCATGY 2 cut(s) 67, 210
XspI CTAG 1 cut(s) 312
Zsp2I ATGCAT 2 cut(s) 148, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.