RLG00000021347
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001688
Physical Location & Seq
Reverse (-)
74352464 .. 74354223
1760 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021347

Sequence Viewer

Length: 780 bp
ATGCCGTTGCTGGTCCGCTGGAACATCTTCACCGCACATCATGATTATGGTGATTCCGGAAAAACCCTCTATTGGTTGACTCACTCGTCTAGATGGCTCTTACATGACTCGGAGCTTACTCTATCTCCGATGAAAGCGCACGGGCGTGACATTTGGCAGTTCTTCTCCGAAAACACAAAGTACAACCAATTGTTCAACGATGGCATGGTGTGCACTGCTAAGATTACAATGAAAGCGATACTAGCGGGGTATAAGGGTGCGTTTGATGACGTGGCAACACTGATGGATATTGGTGGTGGGATTGGAAGCTCAGTGGCGGAGATTGTGAAGTTGTATCCCAGCATCAAGGGCATCAACTTTGATCTACCACATGTAGTTGCAACAGCGTCGGTGTACCATGGGGTATCACACGTTGGAGGTGACATGTTTGATGAGGGAAATATTCCAAACGCTGATGCGATTTTCATGAAGAGAAGTGGAAAGGTGATTATTGTGGACATTGTTCTAGAGCTAAATGGTAATGGCATGTTCGACGACACAAGCTTGGAGAGGACTGAGATTGAATGGAAGAACATGTTGGAACAAGCAGGCTTCCCTCGCTACAAAATCATCAAAATTCCAGCTTTACTGTCCATTATTCAGGCATACCCTATGTCAAAAGTTAAATCTTATTATAGCCAAGTATTAATAAAATGTGTGCATGGTTATGTTACAATGTCTGCTCAAGATCAATTAGGTAGTAAAATAGTGGCAACATGGGGCACTTGTTCTTCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

260

Amino Acids

28.79

Weight (kDa)

6.49

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 45 - 158 4.9e-29 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 4 cut(s) 16, 33, 245, 317
AcsI RAATTY 1 cut(s) 615
AfaI GTAC 2 cut(s) 182, 395
AfiI CCNNNNNNNGG 1 cut(s) 72
AflIII ACRYGT 4 cut(s) 370, 409, 423, 573
AgsI TTSAA 2 cut(s) 196, 563
AjiI CACGTC 1 cut(s) 271
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 5 cut(s) 115, 309, 511, 543, 623
AluI AGCT 5 cut(s) 115, 309, 511, 543, 623
Alw21I GWGCWC 1 cut(s) 215
Alw44I GTGCAC 1 cut(s) 211
Aor13HI TCCGGA 1 cut(s) 56
ApaLI GTGCAC 1 cut(s) 211
ApoI RAATTY 1 cut(s) 615
AseI ATTAAT 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 4 cut(s) 22, 62, 431, 496
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 2 cut(s) 215, 764
Bbv12I GWGCWC 1 cut(s) 215
BccI CCATC 3 cut(s) 87, 194, 277
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciVI GTATCC 1 cut(s) 345
BfaI CTAG 3 cut(s) 90, 242, 506
BfuI GTATCC 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 13
BmsI GCATC 3 cut(s) 351, 360, 445
BpuEI CTTGAG 1 cut(s) 708
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 397
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 2 cut(s) 324, 546
BseSI GKGCMC 2 cut(s) 215, 764
BseYI CCCAGC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 215
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 215, 764
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 2 cut(s) 361, 727
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 4 cut(s) 16, 33, 245, 317
BspCNI CTCAG 2 cut(s) 323, 547
BspEI TCCGGA 1 cut(s) 56
BspHI TCATGA 2 cut(s) 40, 465
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 2 cut(s) 361, 727
BssT1I CCWWGG 1 cut(s) 397
Bst4CI ACNGT 1 cut(s) 630
Bst6I CTCTTC 1 cut(s) 464
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 219, 310, 555
BstDSI CCRYGG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 2 cut(s) 364, 730
BstMBI GATC 2 cut(s) 361, 727
BstMWI GCNNNNNNNGC 4 cut(s) 210, 242, 348, 597
BstNSI RCATGY 4 cut(s) 374, 427, 529, 577
BstSLI GKGCMC 2 cut(s) 215, 764
BsuI GTATCC 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 397
BtrI CACGTC 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 3 cut(s) 213, 278, 318
Cac8I GCNNGC 1 cut(s) 589
CciI TCATGA 2 cut(s) 40, 465
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 13
CseI GACGC 1 cut(s) 375
Csp6I GTAC 2 cut(s) 181, 394
CviJI RGCY 8 cut(s) 97, 115, 309, 511, 543, 591, 623, 678
CviKI_1 RGCY 8 cut(s) 97, 115, 309, 511, 543, 591, 623, 678
CviQI GTAC 2 cut(s) 181, 394
DdeI CTNAG 3 cut(s) 219, 310, 555
DpnI GATC 2 cut(s) 363, 729
DpnII GATC 2 cut(s) 361, 727
DrdI GACNNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 464
EciI GGCGGA 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FauI CCCGC 1 cut(s) 238
FspBI CTAG 3 cut(s) 90, 242, 506
GlaI GCGC 1 cut(s) 138
GsaI CCCAGC 1 cut(s) 342
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 139
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 3 cut(s) 53, 79, 107
HpaII CCGG 1 cut(s) 57
HphI GGTGA 4 cut(s) 22, 62, 431, 496
Hpy166II GTNNAC 4 cut(s) 78, 213, 394, 496
Hpy188I TCNGA 3 cut(s) 112, 129, 169
Hpy188III TCNNGA 6 cut(s) 41, 57, 90, 466, 506, 725
Hpy8I GTNNAC 4 cut(s) 78, 213, 394, 496
Hpy99I CGWCG 2 cut(s) 391, 536
HpyCH4III ACNGT 1 cut(s) 630
HpyCH4IV ACGT 2 cut(s) 270, 411
HpyCH4V TGCA 3 cut(s) 213, 380, 700
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 242, 348, 597
HpyF3I CTNAG 3 cut(s) 219, 310, 555
HpySE526I ACGT 2 cut(s) 270, 411
HspAI GCGC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 56
Kzo9I GATC 2 cut(s) 361, 727
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 6 cut(s) 4, 70, 352, 573, 626, 633
LweI GCATC 3 cut(s) 351, 360, 445
MaeI CTAG 3 cut(s) 90, 242, 506
MaeII ACGT 2 cut(s) 270, 411
MaeIII GTNAC 3 cut(s) 146, 419, 709
MalI GATC 2 cut(s) 363, 729
MboI GATC 2 cut(s) 361, 727
MboII GAAGA 5 cut(s) 19, 154, 481, 580, 762
MfeI CAATTG 1 cut(s) 188
MhlI GDGCHC 2 cut(s) 215, 764
MluCI AATT 4 cut(s) 188, 615, 731, 775
MlyI GAGTC 2 cut(s) 73, 101
MmeI TCCRAC 2 cut(s) 394, 558
MnlI CCTC 5 cut(s) 77, 410, 427, 543, 606
MroI TCCGGA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 2 cut(s) 663, 686
MslI CAYNNNNRTG 3 cut(s) 45, 144, 705
MspA1I CMGCKG 1 cut(s) 18
MspI CCGG 1 cut(s) 57
MunI CAATTG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 210, 242, 348, 597
NcoI CCATGG 1 cut(s) 397
NdeII GATC 2 cut(s) 361, 727
NmuCI GTSAC 2 cut(s) 146, 419
NspI RCATGY 4 cut(s) 374, 427, 529, 577
OliI CACNNNNGTG 1 cut(s) 144
PagI TCATGA 2 cut(s) 40, 465
PciI ACATGT 3 cut(s) 370, 423, 573
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 53
PleI GAGTC 2 cut(s) 73, 101
PpsI GAGTC 2 cut(s) 73, 101
PscI ACATGT 3 cut(s) 370, 423, 573
PshBI ATTAAT 1 cut(s) 686
PspFI CCCAGC 1 cut(s) 338
PspPI GGNCC 1 cut(s) 13
RsaI GTAC 2 cut(s) 182, 395
RsaNI GTAC 2 cut(s) 181, 394
RseI CAYNNNNRTG 3 cut(s) 45, 144, 705
SaqAI TTAA 2 cut(s) 663, 686
Sau3AI GATC 2 cut(s) 361, 727
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 2 cut(s) 73, 101
SduI GDGCHC 2 cut(s) 215, 764
SfaNI GCATC 3 cut(s) 351, 360, 445
SinI GGWCC 1 cut(s) 13
SmiMI CAYNNNNRTG 3 cut(s) 45, 144, 705
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
Sse9I AATT 4 cut(s) 188, 615, 731, 775
SsiI CCGC 4 cut(s) 16, 33, 245, 317
SspI AATATT 1 cut(s) 442
SspMI CTAG 3 cut(s) 90, 242, 506
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 630
TaiI ACGT 2 cut(s) 273, 414
TaqI TCGA 1 cut(s) 531
TasI AATT 4 cut(s) 188, 615, 731, 775
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 2 cut(s) 663, 686
Tru9I TTAA 2 cut(s) 663, 686
TscAI CASTG 3 cut(s) 220, 285, 318
TseFI GTSAC 2 cut(s) 146, 419
Tsp45I GTSAC 2 cut(s) 146, 419
TspDTI ATGAA 4 cut(s) 146, 245, 454, 482
TspRI CASTG 3 cut(s) 220, 285, 318
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 13
VspI ATTAAT 1 cut(s) 686
XapI RAATTY 1 cut(s) 615
XbaI TCTAGA 2 cut(s) 89, 505
XceI RCATGY 4 cut(s) 374, 427, 529, 577
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 3 cut(s) 90, 242, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.