RchiOBHm_Chr6g0247681

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001302
Physical Location & Seq
Forward (+)
3645120 .. 3645640
521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGAATTGTCTAAGATTTCATTATCCCTCATAGCCTTCGCCCTACTCCATTCCACTCATGCACAAAACTCACCCCAGGACTACCTCAATGCCCACAACAGTGCTCGAGCTGCTGTGGGCGTGGGAGGCTTGACATGGGACACTAATCTAGTTGCTTATGCGCAAAGATATGCTAATTCACATATTGGTGATTGCAACATGGTGCATTCTGGTGGGCCTTATGGCGAAAACCTTGCAAAGAGCACCGGCGACTTATCAGGCACATCTGCTGTTAACTTGTGGGTGAGAGAGAAGGCATACTATGATTACAACCCTAACACATGTGCTTCTGGCAAGGTCTGTGGGCATTATACGCAGGTCGTTTGGCGCAACTCGGTACGTGTGGGGTGTGCCAAAGTGAGGTGCAACAATGGAGGTACTTTCATCGGGTGCAATTATGATCCCCCGGGAAACTACGTTGGTCAGAAGCCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

158

Amino Acids

17.05

Weight (kDa)

8.52

Isoelectric Point (pI)

20.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 29 - 146 2.6e-18 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 346
AclWI GGATC 1 cut(s) 434
AfaI GTAC 2 cut(s) 378, 418
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 2 cut(s) 320, 379
AjnI CCWGG 1 cut(s) 75
AleI CACNNNNGTG 1 cut(s) 99
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw21I GWGCWC 2 cut(s) 106, 245
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 2 cut(s) 105, 445
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 163, 369
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 2 cut(s) 446, 447
AsuHPI GGTGA 3 cut(s) 63, 200, 295
AvaI CYCGRG 2 cut(s) 105, 445
Bbv12I GWGCWC 2 cut(s) 106, 245
BbvI GCAGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 77
BcnI CCSGG 2 cut(s) 446, 447
BfaI CTAG 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 3 cut(s) 77, 446, 447
BmeT110I CYCGRG 2 cut(s) 105, 445
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 3 cut(s) 77, 446, 447
BpuMI CCSGG 2 cut(s) 446, 447
BsaAI YACGTR 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 75, 444, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse118I RCCGGY 1 cut(s) 245
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 3 cut(s) 75, 444, 445
BseLI CCNNNNNNNGG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 97
BshFI GGCC 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 106, 245
BsiHKCI CYCGRG 2 cut(s) 105, 445
BsiSI CCGG 2 cut(s) 246, 446
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 399
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 1 cut(s) 217
BsoBI CYCGRG 2 cut(s) 105, 445
Bsp1286I GDGCHC 2 cut(s) 106, 245
Bsp143I GATC 1 cut(s) 439
BspANI GGCC 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 245
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 75, 444, 445
BssMI GATC 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 101
BstBAI YACGTR 1 cut(s) 380
BstDEI CTNAG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 163, 369
BstKTI GATC 1 cut(s) 442
BstMBI GATC 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 110, 126, 352
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 3 cut(s) 75, 444, 445
BstV1I GCAGC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 346
CfoI GCGC 2 cut(s) 163, 369
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 215
Cfr9I CCCGGG 1 cut(s) 445
Csp6I GTAC 2 cut(s) 377, 417
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 4 cut(s) 59, 135, 199, 321
CviJI RGCY 5 cut(s) 35, 110, 129, 217, 469
CviKI_1 RGCY 5 cut(s) 35, 110, 129, 217, 469
CviQI GTAC 2 cut(s) 377, 417
DdeI CTNAG 1 cut(s) 12
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
Eco88I CYCGRG 2 cut(s) 105, 445
EcoRII CCWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 62, 138, 202, 324
FaqI GGGAC 1 cut(s) 152
FatI CATG 4 cut(s) 58, 134, 198, 320
Fnu4HI GCNGC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 149
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 2 cut(s) 162, 368
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 217
HapII CCGG 2 cut(s) 246, 446
HhaI GCGC 2 cut(s) 163, 369
Hin1II CATG 4 cut(s) 62, 138, 202, 324
Hin6I GCGC 2 cut(s) 161, 367
HinP1I GCGC 2 cut(s) 161, 367
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 2 cut(s) 246, 446
HphI GGTGA 3 cut(s) 63, 200, 295
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 465
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 2 cut(s) 46, 286
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 379, 456
HpyCH4V TGCA 6 cut(s) 62, 195, 205, 236, 405, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 126, 352
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 379, 456
Hsp92II CATG 4 cut(s) 62, 138, 202, 324
HspAI GCGC 2 cut(s) 161, 367
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 1 cut(s) 439
LpnPI CCDG 8 cut(s) 62, 89, 195, 243, 259, 315, 341, 459
Lsp1109I GCAGC 1 cut(s) 97
MaeI CTAG 1 cut(s) 149
MaeII ACGT 2 cut(s) 379, 456
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 106, 245
MluCI AATT 3 cut(s) 5, 175, 433
MnlI CCTC 5 cut(s) 38, 95, 119, 393, 407
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 3 cut(s) 99, 186, 210
MspI CCGG 2 cut(s) 246, 446
MspR9I CCNGG 3 cut(s) 77, 446, 447
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 110, 126, 352
NciI CCSGG 2 cut(s) 446, 447
NdeII GATC 1 cut(s) 439
NlaIII CATG 4 cut(s) 62, 138, 202, 324
NsbI TGCGCA 1 cut(s) 162
NspI RCATGY 1 cut(s) 324
OliI CACNNNNGTG 1 cut(s) 99
PaeR7I CTCGAG 1 cut(s) 105
PciI ACATGT 1 cut(s) 320
PctI GAATGC 1 cut(s) 205
PkrI GCNGC 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 380
PscI ACATGT 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspPI GGNCC 1 cut(s) 215
PspXI VCTCGAGB 1 cut(s) 105
RsaI GTAC 2 cut(s) 378, 418
RsaNI GTAC 2 cut(s) 377, 417
RseI CAYNNNNRTG 3 cut(s) 99, 186, 210
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 1 cut(s) 215
ScrFI CCNGG 3 cut(s) 77, 446, 447
SduI GDGCHC 2 cut(s) 106, 245
SetI ASST 9 cut(s) 87, 112, 234, 339, 360, 382, 404, 418, 459
Sfr274I CTCGAG 1 cut(s) 105
SgrAI CRCCGGYG 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 105
SmaI CCCGGG 1 cut(s) 447
SmiMI CAYNNNNRTG 3 cut(s) 99, 186, 210
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
Sse9I AATT 3 cut(s) 5, 175, 433
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 3 cut(s) 75, 444, 445
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 382, 459
TaqI TCGA 1 cut(s) 106
TasI AATT 3 cut(s) 5, 175, 433
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 106
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 9, 412
TspMI CCCGGG 1 cut(s) 445
TspRI CASTG 1 cut(s) 106
XceI RCATGY 1 cut(s) 324
XhoI CTCGAG 1 cut(s) 105
XmaI CCCGGG 1 cut(s) 445
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.