RchiOBHm_Chr6g0254051

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001292
Physical Location & Seq
Forward (+)
9113273 .. 9114913
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1572 bp
ATGTTGTGGGTCCACAAAAACCATCCTTTAACCCTAGCTTTGAATCTCAGGGTTTTTGGGGATTTAGGGCTGTTCAAAGATCTGCTGGGAATTCTCCATAAGGTATTGGAGGAATCCATATGTGGACACTATCTGTGGACTCCATATGGGTACTGGTGGACTCCAAATGGGAGAAATAAAGTTGAAGAGGTAGTTGCAGCATGGAGAAGAGCAGCTGACATTTCAAAGGCTAAAATTGCAGTTGAGAGGTACCAAAATGACCAAAGGTATCAAAACTTGCATAATCAAGTAGCGGATTTGTTTTCAGAGTTCTTGAAATCAGATGTCAAATTTTTGGAATCAGGTGAGATTGAAAACATCAGTTTTGCTGCCAAATTTTGCCCTTCAATTGATTCCAAATATGATAAAGCAACCCTGATATGTGAAGTCATTGCAAAGAGAATTTTCCCACGCGTAGATTATGAAGAATACAAAGATATTTCGGAACCTCATTATGCTTACAGGGTTCGTAATCGATTGCAGAAACAAGTGCTTGTTCCTCTGCACAAAGTGCTACAGTCATCATCAGTGAACAATGCTGTTGAGAGAAAGAAGTCATGGTCCCAAAACCTTAATGCCCTGAATTGTTTGGAAACTTATTGGGAAATCTACAATTATAGGTGCTCCATCATTGATAGGGATTTAAGCCAAAGCAGATTAGAACTATTTAAGCTTTACATGAAGATTATGGACAGGTTGGGCATAAAAATTGATGACAGGCTAAAACTTCCTCACCAAGTAATAGCTTTTTTGAACAATACACAGGGTAGTGATGAAATTGCAGAGCTTCAGTGGCAGAAACTAGTCCAAGGGCTGTCAAACAAAGGGAAGCTCAGGATTTGTCTTGCTGTGTGTGACAGCTCGTTGAGCATGATAGGAACAGACAAGGAAATGGCGTGCATTGCAATGGGATTATTGATTTCAGAACTGAGTGAGAGTCCATGGAATGGAGTGGTTTTCTCATTCACTGATTTTCCCAAAATTCACAAGATTGTAGGAGAAAGTCTTCGATCGAAGTGCGAGTTCATGAGGCAGATGGAGTGTGATGAGAAAGTGGACTGCTTAGAGATTTACAACCGAGTGTTGGAGATTGCCACCACACAAAAGCTAAACCACATAAAGATGCCAAAGAGGGTTTTTGTGTTCACCTACAAGGATTTTGTTGAGGCCTTCAAGTATGATTGGCCGGATGATTACACAGAAGCATATAACAATTTCAGAAAAAGAGGGTTTGAGTTTAATGTGCCAGAGCTAGAGTTTTGGAATTTGAAGGATTCCATTGCCAAGCCAGAGGTGATTTATAGCCCAGTGAAGGAGAACCAGAAAGTGGGGATCATAATAACAGGGTTCTCTAGCAATCTACTCACGTTGTTCTTGAAAGGGGAGGCAGATTCAAGATCATATGCAGCTCAATTCCAGGCACCTTACGGTATTGATTTACAGTCAGTACCAAAACTGCTTCCTAGAGTAGAGGATGTGTTTGATAGTGCAATTTCAAGAGAAGAATTAGAGAGCCTGGTATTGTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

523

Amino Acids

60.82

Weight (kDa)

6.42

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 65 - 291 4e-38 Domain of unknown function (DUF2828)
DUF7788 PF25043 294 - 476 3.6e-40 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 249, 1459
AccB7I CCANNNNNTGG 2 cut(s) 986, 1366
AccII CGCG 1 cut(s) 453
AciI CCGC 1 cut(s) 293
AclWI GGATC 1 cut(s) 1379
AcoI YGGCCR 1 cut(s) 1223
AcsI RAATTY 6 cut(s) 90, 329, 374, 441, 1020, 1303
AcuI CTGAAG 1 cut(s) 812
AdeI CACNNNGTG 1 cut(s) 550
AfaI GTAC 3 cut(s) 152, 251, 1488
AfiI CCNNNNNNNGG 4 cut(s) 986, 1123, 1351, 1366
AflIII ACRYGT 1 cut(s) 451
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 2 cut(s) 1455, 1554
Alw21I GWGCWC 1 cut(s) 665
AlwI GGATC 1 cut(s) 1379
AoxI GGCC 2 cut(s) 1206, 1223
ApeKI GCWGC 4 cut(s) 197, 212, 368, 1445
ApoI RAATTY 6 cut(s) 90, 329, 374, 441, 1020, 1303
Asp700I GAANNNNTTC 1 cut(s) 1044
Asp718I GGTACC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 10, 600
AsuHPI GGTGA 4 cut(s) 356, 764, 1177, 1345
AvaII GGWCC 2 cut(s) 10, 600
BanI GGYRCC 2 cut(s) 249, 1459
BbsI GAAGAC 1 cut(s) 1037
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 4 cut(s) 209, 224, 355, 1457
BccI CCATC 3 cut(s) 30, 674, 1069
BcgI CGANNNNNNTGC 2 cut(s) 1038, 1072
BciT130I CCWGG 2 cut(s) 1457, 1556
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 5 cut(s) 35, 842, 1292, 1392, 1503
BfmI CTRYAG 1 cut(s) 554
BglII AGATCT 1 cut(s) 79
BisI GCNGC 4 cut(s) 198, 213, 369, 1446
BlsI GCNGC 4 cut(s) 199, 214, 370, 1447
Bme1390I CCNGG 2 cut(s) 1457, 1556
Bme18I GGWCC 2 cut(s) 10, 600
BmgT120I GGNCC 2 cut(s) 10, 600
BmiI GGNNCC 5 cut(s) 11, 251, 486, 602, 1461
BmrFI CCNGG 2 cut(s) 1457, 1556
BmrI ACTGGG 1 cut(s) 1340
BmsI GCATC 1 cut(s) 1152
BmuI ACTGGG 1 cut(s) 1340
BpiI GAAGAC 1 cut(s) 1037
Bpu10I CCTNAGC 1 cut(s) 872
Bsa29I ATCGAT 1 cut(s) 514
BsaJI CCNNGG 2 cut(s) 847, 980
Bsc4I CCNNNNNNNGG 4 cut(s) 986, 1123, 1351, 1366
Bse1I ACTGG 2 cut(s) 158, 1346
Bse3DI GCAATG 4 cut(s) 429, 939, 951, 1317
BseBI CCWGG 2 cut(s) 1457, 1556
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 847, 980
BseGI GGATG 3 cut(s) 22, 1234, 1519
BseLI CCNNNNNNNGG 4 cut(s) 986, 1123, 1351, 1366
BseMI GCAATG 4 cut(s) 429, 939, 951, 1317
BseMII CTCAG 3 cut(s) 61, 886, 959
BseNI ACTGG 2 cut(s) 158, 1346
BseXI GCAGC 4 cut(s) 209, 224, 355, 1457
BseYI CCCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 527
Bsh1236I CGCG 1 cut(s) 453
Bsh1285I CGRYCG 1 cut(s) 1052
BshFI GGCC 2 cut(s) 1208, 1225
BshNI GGYRCC 2 cut(s) 249, 1459
BshVI ATCGAT 1 cut(s) 514
BsiEI CGRYCG 1 cut(s) 1052
BsiHKAI GWGCWC 1 cut(s) 665
BsiSI CCGG 1 cut(s) 1226
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 4 cut(s) 986, 1123, 1351, 1366
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 2 cut(s) 1208, 1225
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 4 cut(s) 79, 1049, 1371, 1436
Bsp19I CCATGG 1 cut(s) 980
BspACI CCGC 1 cut(s) 293
BspANI GGCC 2 cut(s) 1208, 1225
BspCNI CTCAG 3 cut(s) 60, 885, 960
BspDI ATCGAT 1 cut(s) 514
BspFNI CGCG 1 cut(s) 453
BspHI TCATGA 1 cut(s) 1065
BspLI GGNNCC 5 cut(s) 11, 251, 486, 602, 1461
BspPI GGATC 1 cut(s) 1379
BspQI GCTCTTC 1 cut(s) 202
BspT107I GGYRCC 2 cut(s) 249, 1459
BsrDI GCAATG 4 cut(s) 429, 939, 951, 1317
BsrI ACTGG 2 cut(s) 158, 1346
BssECI CCNNGG 2 cut(s) 847, 980
BssMI GATC 4 cut(s) 79, 1049, 1371, 1436
BssT1I CCWWGG 2 cut(s) 847, 980
Bst2UI CCWGG 2 cut(s) 1457, 1556
Bst4CI ACNGT 3 cut(s) 558, 1469, 1482
Bst6I CTCTTC 2 cut(s) 180, 202
BstAPI GCANNNNNTGC 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 937
BstDEI CTNAG 4 cut(s) 47, 872, 968, 1102
BstDSI CCRYGG 1 cut(s) 980
BstF5I GGATG 3 cut(s) 22, 1234, 1519
BstFNI CGCG 1 cut(s) 453
BstKTI GATC 4 cut(s) 82, 1052, 1374, 1439
BstMBI GATC 4 cut(s) 79, 1049, 1371, 1436
BstMCI CGRYCG 1 cut(s) 1052
BstMWI GCNNNNNNNGC 5 cut(s) 236, 550, 832, 906, 941
BstNI CCWGG 2 cut(s) 1457, 1556
BstSCI CCNGG 2 cut(s) 1455, 1554
BstSFI CTRYAG 1 cut(s) 554
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 4 cut(s) 209, 224, 355, 1457
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 514
BsuRI GGCC 2 cut(s) 1208, 1225
BsuTUI ATCGAT 1 cut(s) 514
BtgI CCRYGG 1 cut(s) 980
BtsCI GGATG 3 cut(s) 22, 1234, 1519
BtsIMutI CAGTG 4 cut(s) 573, 836, 1005, 1353
Cac8I GCNNGC 1 cut(s) 937
CciI TCATGA 1 cut(s) 1065
Cfr13I GGNCC 2 cut(s) 10, 600
ClaI ATCGAT 1 cut(s) 514
Csp6I GTAC 3 cut(s) 151, 250, 1487
CviAII CATG 6 cut(s) 201, 597, 718, 910, 981, 1066
CviQI GTAC 3 cut(s) 151, 250, 1487
DdeI CTNAG 4 cut(s) 47, 872, 968, 1102
DpnI GATC 4 cut(s) 81, 1051, 1373, 1438
DpnII GATC 4 cut(s) 79, 1049, 1371, 1436
DraIII CACNNNGTG 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 1223
Eam1104I CTCTTC 2 cut(s) 180, 202
EarI CTCTTC 2 cut(s) 180, 202
Eco130I CCWWGG 2 cut(s) 847, 980
Eco147I AGGCCT 1 cut(s) 1208
Eco47I GGWCC 2 cut(s) 10, 600
Eco57I CTGAAG 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 1455, 1554
EcoT14I CCWWGG 2 cut(s) 847, 980
ErhI CCWWGG 2 cut(s) 847, 980
FaeI CATG 6 cut(s) 204, 600, 721, 913, 984, 1069
FaqI GGGAC 1 cut(s) 586
FatI CATG 6 cut(s) 200, 596, 717, 909, 980, 1065
FauNDI CATATG 3 cut(s) 119, 145, 1441
Fnu4HI GCNGC 4 cut(s) 198, 213, 369, 1446
FokI GGATG 3 cut(s) 9, 1241, 1526
Fsp4HI GCNGC 4 cut(s) 198, 213, 369, 1446
FspBI CTAG 5 cut(s) 35, 842, 1292, 1392, 1503
GluI GCNGC 4 cut(s) 198, 213, 369, 1446
GsaI CCCAGC 1 cut(s) 89
HaeIII GGCC 2 cut(s) 1208, 1225
HapII CCGG 1 cut(s) 1226
Hin1II CATG 6 cut(s) 204, 600, 721, 913, 984, 1069
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 9 cut(s) 43, 113, 139, 160, 338, 392, 976, 1313, 1430
HpaII CCGG 1 cut(s) 1226
HphI GGTGA 4 cut(s) 356, 764, 1177, 1345
Hpy166II GTNNAC 7 cut(s) 13, 125, 138, 159, 571, 1096, 1185
Hpy188I TCNGA 5 cut(s) 307, 322, 484, 964, 1259
Hpy188III TCNNGA 6 cut(s) 313, 874, 1066, 1414, 1434, 1536
Hpy8I GTNNAC 7 cut(s) 13, 125, 138, 159, 571, 1096, 1185
HpyAV CCTTC 4 cut(s) 393, 1219, 1303, 1345
HpyCH4III ACNGT 3 cut(s) 558, 1469, 1482
HpyCH4IV ACGT 1 cut(s) 1406
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 550, 832, 906, 941
HpyF3I CTNAG 4 cut(s) 47, 872, 968, 1102
HpySE526I ACGT 1 cut(s) 1406
Hsp92II CATG 6 cut(s) 204, 600, 721, 913, 984, 1069
KpnI GGTACC 1 cut(s) 253
Kzo9I GATC 4 cut(s) 79, 1049, 1371, 1436
LguI GCTCTTC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 668
Lsp1109I GCAGC 4 cut(s) 209, 224, 355, 1457
LweI GCATC 1 cut(s) 1152
MaeI CTAG 5 cut(s) 35, 842, 1292, 1392, 1503
MaeII ACGT 1 cut(s) 1406
MaeIII GTNAC 1 cut(s) 893
MalI GATC 4 cut(s) 81, 1051, 1373, 1438
MboI GATC 4 cut(s) 79, 1049, 1371, 1436
MboII GAAGA 6 cut(s) 197, 219, 476, 733, 1037, 1553
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 1 cut(s) 665
MluI ACGCGT 1 cut(s) 451
MlyI GAGTC 3 cut(s) 133, 154, 985
MmeI TCCRAC 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 1044
MseI TTAA 5 cut(s) 29, 612, 683, 708, 1278
MslI CAYNNNNRTG 2 cut(s) 944, 1160
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 1226
MspR9I CCNGG 2 cut(s) 1457, 1556
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 2 cut(s) 1457, 1556
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 5 cut(s) 236, 550, 832, 906, 941
NcoI CCATGG 1 cut(s) 980
NdeI CATATG 3 cut(s) 119, 145, 1441
NdeII GATC 4 cut(s) 79, 1049, 1371, 1436
NlaIII CATG 6 cut(s) 204, 600, 721, 913, 984, 1069
NlaIV GGNNCC 5 cut(s) 11, 251, 486, 602, 1461
NmuCI GTSAC 1 cut(s) 893
PagI TCATGA 1 cut(s) 1065
PceI AGGCCT 1 cut(s) 1208
PciSI GCTCTTC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 6 cut(s) 43, 113, 338, 392, 1313, 1430
PflMI CCANNNNNTGG 2 cut(s) 986, 1366
PkrI GCNGC 4 cut(s) 199, 214, 370, 1447
Ple19I CGATCG 1 cut(s) 1052
PleI GAGTC 3 cut(s) 133, 154, 984
PpsI GAGTC 3 cut(s) 133, 154, 984
Psp6I CCWGG 2 cut(s) 1455, 1554
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 2 cut(s) 1455, 1554
PspN4I GGNNCC 5 cut(s) 11, 251, 486, 602, 1461
PspPI GGNCC 2 cut(s) 10, 600
PsuI RGATCY 1 cut(s) 79
PvuI CGATCG 1 cut(s) 1052
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 3 cut(s) 152, 251, 1488
RsaNI GTAC 3 cut(s) 151, 250, 1487
RseI CAYNNNNRTG 2 cut(s) 944, 1160
SapI GCTCTTC 1 cut(s) 202
SaqAI TTAA 5 cut(s) 29, 612, 683, 708, 1278
SatI GCNGC 4 cut(s) 198, 213, 369, 1446
Sau3AI GATC 4 cut(s) 79, 1049, 1371, 1436
Sau96I GGNCC 2 cut(s) 10, 600
SchI GAGTC 3 cut(s) 133, 154, 985
ScrFI CCNGG 2 cut(s) 1457, 1556
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 1 cut(s) 1152
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 2 cut(s) 10, 600
SmiMI CAYNNNNRTG 2 cut(s) 944, 1160
SpeI ACTAGT 1 cut(s) 841
SseBI AGGCCT 1 cut(s) 1208
SsiI CCGC 1 cut(s) 293
SspMI CTAG 5 cut(s) 35, 842, 1292, 1392, 1503
StuI AGGCCT 1 cut(s) 1208
StyD4I CCNGG 2 cut(s) 1455, 1554
StyI CCWWGG 2 cut(s) 847, 980
TaaI ACNGT 3 cut(s) 558, 1469, 1482
TaiI ACGT 1 cut(s) 1409
TaqI TCGA 3 cut(s) 514, 1048, 1052
TfiI GAWTC 6 cut(s) 43, 113, 338, 392, 1313, 1430
Tru1I TTAA 5 cut(s) 29, 612, 683, 708, 1278
Tru9I TTAA 5 cut(s) 29, 612, 683, 708, 1278
TscAI CASTG 4 cut(s) 573, 836, 1012, 1353
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 4 cut(s) 197, 212, 368, 1445
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 4 cut(s) 477, 734, 828, 1054
TspRI CASTG 4 cut(s) 573, 836, 1012, 1353
Van91I CCANNNNNTGG 2 cut(s) 986, 1366
VpaK11BI GGWCC 2 cut(s) 10, 600
XapI RAATTY 6 cut(s) 90, 329, 374, 441, 1020, 1303
XcmI CCANNNNNNNNNTGG 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 5 cut(s) 35, 842, 1292, 1392, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.